Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0006249

Physical Location & Seq

Genomic Coordinates Forward (+)

26689504 .. 26698447

Gene Structure 8944 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g36460.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2982 bp

ATGAGGATTGACGAAGAGGCCGACGGAGTCTCCGCCTCCAGGCACCACCTCGTCTTCGCTTACTACGTCACCGGCCACGGCTTCGGCCACGCCACTCGCGTCGTCGAGGTGGTGCGGCACCTGATCCTCGCCGGCCATGACGTGCATGTCGTCACCGGAGCTCCTGACTTTGTTTTCACCTCGGAGATTCAGTCGCCTCGGCTCTTCATCCGCAAGGTGCTGCTGGACTGTGGAGCGGTTCAGGCGGATGCATTGACAGTCGATCGCCTTGCATCTTTGGAGAAGTATTCGGAGACGGCTGTGGTGCCTAGGGAGTCTATATTGAAAACTGAAGTAGAGTGGCTGACCTCCATCAAAGCTGACCTGGTGGTTTCAGATGTTGTTCCAGTTGCGTGCCGAGCTGCAGCCGATGCTGGGATTCGCTCTGTCTGTGTCACTAACTTCAGCTGGGACTTCATCTATGCTGAGTATGTCATGGCTGCCGGAAGTCATCATCGTACTATAGTTTGGCAGATAGCAGAAGATTATTCTCACTGTGAGTTCCTGATTCGCCTCCCAGGATACTGCCCAATGCCTGCATTCCGTGATGTAATTGACGTACCTTTAGTTGTGAGGAGGTTACACAGATCCCGCAAAGAGGTGAGGAAGGAACTGGAAATTGAAGAAGATGTCAAATTAGTCATTCTGAACTTTGGTGGACAGCCATCAGGCTGGAAGTTGAAAGAGGAGTTTCTACCCCCTGGATGGCTTGGCCTGCTCTGTGGTGCTTCGGAGAGCCAGGAGCTTCCACCAAATTTTAGGAAGCTTGCAAAAGATGCTTATACTCCTGATATCATAGCGGCATCCGATTGTATGCTGGGAAAAATTGGATATGGCACTGTGAGTGAAGCCCTTGCATTCAAATTACCATTTGTCTTTGTACGCAGAGATTATTTCAATGAAGAACCTTTTTTGAGAAATATGCTTGAGTATTATCAATCCGGAGTTGAGATGATTAGGAGAGATCTGCTCACTGGTCATTGGAGACCTTACCTTGAGCGTGCAATCAGTTTAAAACCTTGCTATGAAGGAGGCACCAATGGTGGTGAGGTGGCAGCTCAGGTCCTTCAAGAAACAGCTATTGGGAAAAACTGGGCATCAGATAAGCTTAGTGGGGCAAGAAGGTTGCGAGATGCTATAATTCTTGGGTATCAACTTCAAAGGGTACCTGGACGAGAAATGGCCATCCCCGAGTGGTATGCAAATGCTGAAACTGAACTTCGAATTGGATCACCAACTTGCCAAATGAGTGAGACTGATGAGAAGAGCTCCCTAATGAACTCATGCATTGAAGACTTTGATATTCTTCATGGAGATCTTCAAGGTCTTTCCGATACAATGACTTTTCTGAAGAGCTTGGCAGAATTAGATTCTGCGTATGAATCTGAAAAAGCTACTGAGAAGCGGCGGAAGAGGGAACGGAAGGCTGCAGCTGGACTCTTCAATTGGGAGGAAGATATTTTTGTGGCAAGAGCGCCTGGACGATTAGATGTAATGGGTGGGATTGCTGACTATTCAGGAAGCCTTGTCTTGCAGATGCCCATAAGGGAAGCTTGCCATGTTGCTGTACAGAGACATCAACCAAGTAAACATAGGCTCTGGAAGCATGCCCTGGCTCGTCAGGAAGCCAAAGGACAGAGCTCCACACCTGTCCTGCAAATTGTCTCATATGGTTCAGAGTTGAGCAATCGTAGTCCTACTTTTGACATGGATCTATCTGATTTTATGGACGGAGATCATCCAATCTCATATGAGAAGGCAAAAATATATTTTTCACAAGATCCGTCTCAAAAGTGGGCAGCATATGTTGCGGGTGTCATTCTGGTTTTAATGACAGAACTAGGTGTACGCTTTGAGGATAGCATTAGTTTGTTGGTATCCTCTTTAGTTCCGGAAGGCAAAGGGGTCTCTTCTTCTGCTTCGATAGAGGTTGCTACAATGTCTGCTATAGCGGCTGCCCATGGATTAAACATCAGTCCCAGAGATCTTGCTTTACTTTGCCAGAAGGTGGAGAATCATATTGTTGGAGCTCCGTGCGGTGTGATGGACCAAATGACTTCTGCATGTGGTGAAGCCAACAAACTTCTTGCGATGGTCTGCCAGCCTGCTGAGGTACTTGGGCTTGTAGAAATTCCTAGCCATGTCCGATTTTGGGGAATCGATTCAGGCATTAGACACAGTGTTGGTGGTGCGGATTATGGATCTGTTAGGATAGGTGCCTTTATGGGTCGGACGATCATCAAGTCTACAGCTTCTACAATTATGTCTAAATCATTGTCCAATAGCAATGGGATGAATGCTGACGAGTTAGAGGATGATGGTCTTGAACTGCCTAAAGCTGAAGCTTCATTAGATTATTTGTGCAACCTGTCACCTCATAGATACGAGGATCTTTATGTTAAGATACTTCCCGAGTCCATACTTGGTGAGGCTTTTCTGGATAAGTATGTTGATCATAGTGACCCAGTTACAGTTATTGATCCGAAGCGTAACTATGGAGTGCGAGCGCCTACTAGACATCCTATATATGAAAATTTTCGAGTTACGGCATTCAAAGCACTTTTAACATCTGTAAATTCAGATTATCAGCTTGCGGCTCTTGGAGAGTTATTGTATCAGTGCCACTACAGCTACAGCGCCTGTGGCCTTGGTTCCGATGGAACAGATAGGCTTGTACAGTTGGTACAGGAGATGCAGCATAGTAAATCATCTAAATTAGACGGCGGGGCTTTATACGGAGCAAAGATTACTGGTGGAGGTTCTGGTGGAACAGTTTGCGTGGTTGGCAGGAATTGCCTCAAGAGCAGCCAGCAAATATTTGAGATTCAGCAGAGATACAAAGCTGCAACAGGGTATATGCCATTTATTTTTGAGGGCTCATCACCAGGTGCAGGAAAGTTTGGCCATCTAAGAATCCGGCGTCGCACTGTCAAGCTAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0005975 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009506 GO:0009702 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0030054 GO:0044237 GO:0044238 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046835 GO:0055044 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

GHMP_kinases_C GHMP_kinases_N GalKase_gal_bdg Glyco_trans_1_3

Protein Analysis

994

Amino Acids

109.7

Weight (kDa)

5.96

Isoelectric Point (pI)

43.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GalKase_gal_bdg	PF10509	497 - 538	5e-07	Galactokinase galactose-binding signature
GHMP_kinases_N	PF00288	615 - 703	3.1e-17	GHMP kinases N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G42850 AT3G42850 AT4G16130
fragaria_vesca	FvH4_2g36460 FvH4_2g36460 FvH4_2g36460
malus_domestica	MD08G1124400.v1.1 MD15G1103100.v1.1
prunus_persica	Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1
pyrus_communis	pycom08g10310 pycom15g09400
rosa_chinensis	RchiOBHm_Chr6g0305421
rosa_laevigata	RLG00000010892
rosa_multiflora	Rmu_sc0002782.1_g000020
rosa_roxburghii	Rroxscaffold_7G00162820
rosa_rugosa	Rorug06G0340200
rosa_samantha	Rh6AG452200 Rh6BG449500 Rh6CG466000 Rh6DG452700
rosa_wichuraiana	Rw6G039400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	146
Acc65I	GGTACC	1 cut(s)	1204
AccB1I	GGYRCC	6 cut(s)	42, 117, 304, 1073, 1204, 2255
AccB7I	CCANNNNNTGG	1 cut(s)	2047
AccBSI	CCGCTC	1 cut(s)	236
AccI	GTMKAC	1 cut(s)	2285
AccII	CGCG	1 cut(s)	99
AccIII	TCCGGA	2 cut(s)	980, 1930
AclWI	GGATC	8 cut(s)	118, 621, 1276, 1758, 1814, 2248, 2436, 2513
AcoI	YGGCCR	5 cut(s)	73, 85, 133, 1221, 2941
AcsI	RAATTY	4 cut(s)	793, 2169, 2572, 2614
AcuI	CTGAAG	4 cut(s)	351, 427, 1409, 2400
AcyI	GRCGYC	1 cut(s)	2959
AdeI	CACNNNGTG	1 cut(s)	2927
AfaI	GTAC	9 cut(s)	499, 600, 921, 1206, 1608, 1887, 2154, 2715, 2724
AfiI	CCNNNNNNNGG	7 cut(s)	39, 414, 637, 744, 2047, 2191, 2930
AjiI	CACGTC	1 cut(s)	142
AjnI	CCWGG	9 cut(s)	38, 363, 556, 739, 777, 1207, 1516, 1650, 2923
AjuI	GAANNNNNNNTTGG	4 cut(s)	1104, 1136, 1248, 1280
Alw21I	GWGCWC	4 cut(s)	163, 1310, 1682, 2071
Alw26I	GTCTC	8 cut(s)	34, 287, 1018, 1286, 1606, 1708, 1830, 1951
AlwI	GGATC	8 cut(s)	118, 621, 1276, 1758, 1814, 2248, 2436, 2513
AlwNI	CAGNNNCTG	1 cut(s)	2679
Ama87I	CYCGRG	2 cut(s)	1229, 2450
Aor13HI	TCCGGA	2 cut(s)	980, 1930
AoxI	GGCC	8 cut(s)	18, 73, 85, 133, 751, 1221, 2683, 2941
ApoI	RAATTY	4 cut(s)	793, 2169, 2572, 2614
Asp700I	GAANNNNTTC	2 cut(s)	2200, 2574
Asp718I	GGTACC	1 cut(s)	1204
AspA2I	CCTAGG	1 cut(s)	308
AspLEI	GCGC	3 cut(s)	1516, 2548, 2678
AspS9I	GGNCC	2 cut(s)	1102, 2086
AsuII	TTCGAA	1 cut(s)	1261
AvaI	CYCGRG	2 cut(s)	1229, 2450
AvaII	GGWCC	2 cut(s)	1102, 2086
AvrII	CCTAGG	1 cut(s)	308
BalI	TGGCCA	2 cut(s)	1223, 2943
BanI	GGYRCC	6 cut(s)	42, 117, 304, 1073, 1204, 2255
BanII	GRGCYC	5 cut(s)	163, 1310, 1682, 2071, 2918
BbsI	GAAGAC	2 cut(s)	46, 1338
Bbv12I	GWGCWC	4 cut(s)	163, 1310, 1682, 2071
BbvCI	CCTCAGC	1 cut(s)	2148
BccI	CCATC	9 cut(s)	359, 712, 738, 1232, 2077, 2125, 2351, 2690, 2952
BceAI	ACGGC	4 cut(s)	94, 312, 2601, 2776
BcgI	CGANNNNNNTGC	4 cut(s)	251, 285, 1220, 1254
BciT130I	CCWGG	9 cut(s)	40, 365, 558, 741, 779, 1209, 1518, 1652, 2925
BciVI	GTATCC	2 cut(s)	554, 1927
BclI	TGATCA	1 cut(s)	2491
BcoDI	GTCTC	8 cut(s)	34, 287, 1018, 1286, 1606, 1708, 1830, 1951
BfaI	CTAG	4 cut(s)	309, 1880, 2175, 2553
BfmI	CTRYAG	7 cut(s)	402, 501, 1467, 1986, 2286, 2665, 2671
BfoI	RGCGCY	3 cut(s)	1517, 2549, 2679
BfuI	GTATCC	2 cut(s)	554, 1927
BglII	AGATCT	3 cut(s)	1003, 1354, 2023
BlnI	CCTAGG	1 cut(s)	308
Bme1390I	CCNGG	9 cut(s)	40, 365, 558, 741, 779, 1209, 1518, 1652, 2925
Bme18I	GGWCC	2 cut(s)	1102, 2086
BmeT110I	CYCGRG	2 cut(s)	1229, 2450
BmgBI	CACGTC	1 cut(s)	142
BmgT120I	GGNCC	2 cut(s)	1102, 2086
BmiI	GGNNCC	7 cut(s)	44, 119, 306, 1075, 1206, 2257, 2692
BmrFI	CCNGG	9 cut(s)	40, 365, 558, 741, 779, 1209, 1518, 1652, 2925
BmrI	ACTGGG	2 cut(s)	1141, 2498
BmsI	GCATC	9 cut(s)	238, 281, 400, 805, 851, 1145, 1162, 1566, 2721
BmuI	ACTGGG	2 cut(s)	1141, 2498
BpiI	GAAGAC	2 cut(s)	46, 1338
BplI	GAGNNNNNCTC	4 cut(s)	993, 1025, 1292, 1324
BpmI	CTGGAG	1 cut(s)	22
Bpu10I	CCTNAGC	2 cut(s)	1098, 2148
Bpu14I	TTCGAA	1 cut(s)	1261
BpuEI	CTTGAG	3 cut(s)	986, 1055, 2822
Bsa29I	ATCGAT	1 cut(s)	2199
BsaHI	GRCGYC	1 cut(s)	2959
BsaI	GGTCTC	2 cut(s)	1018, 1951
BsaJI	CCNNGG	9 cut(s)	76, 180, 197, 308, 556, 739, 1650, 1999, 2686
BsaWI	WCCGGW	3 cut(s)	155, 980, 1930
BsaXI	ACNNNNNCTCC	6 cut(s)	14, 44, 145, 175, 176, 206
Bsc4I	CCNNNNNNNGG	7 cut(s)	39, 414, 637, 744, 2047, 2191, 2930
Bse118I	RCCGGY	2 cut(s)	71, 131
Bse1I	ACTGG	6 cut(s)	386, 657, 1018, 1136, 2504, 2794
Bse3DI	GCAATG	1 cut(s)	2332
BseAI	TCCGGA	2 cut(s)	980, 1930
BseBI	CCWGG	9 cut(s)	40, 365, 558, 741, 779, 1209, 1518, 1652, 2925
BseCI	ATCGAT	1 cut(s)	2199
BseDI	CCNNGG	9 cut(s)	76, 180, 197, 308, 556, 739, 1650, 1999, 2686
BseGI	GGATG	9 cut(s)	207, 253, 749, 842, 1224, 1777, 2337, 2359, 2557
BseLI	CCNNNNNNNGG	7 cut(s)	39, 414, 637, 744, 2047, 2191, 2930
BseMI	GCAATG	1 cut(s)	2332
BseMII	CTCAG	4 cut(s)	456, 1112, 1428, 2139
BseNI	ACTGG	6 cut(s)	386, 657, 1018, 1136, 2504, 2794
BseRI	GAGGAG	2 cut(s)	628, 740
BseYI	CCCAGC	3 cut(s)	413, 447, 856
BsgI	GTGCAG	1 cut(s)	2949
Bsh1236I	CGCG	1 cut(s)	99
Bsh1285I	CGRYCG	1 cut(s)	265
BshFI	GGCC	8 cut(s)	20, 75, 87, 135, 753, 1223, 2685, 2943
BshNI	GGYRCC	6 cut(s)	42, 117, 304, 1073, 1204, 2255
BshVI	ATCGAT	1 cut(s)	2199
BsiEI	CGRYCG	1 cut(s)	265
BsiHKAI	GWGCWC	4 cut(s)	163, 1310, 1682, 2071
BsiHKCI	CYCGRG	2 cut(s)	1229, 2450
BsiSI	CCGG	7 cut(s)	72, 132, 156, 483, 981, 1931, 2956
BslFI	GGGAC	2 cut(s)	464, 2001
BslI	CCNNNNNNNGG	7 cut(s)	39, 414, 637, 744, 2047, 2191, 2930
BsmAI	GTCTC	8 cut(s)	34, 287, 1018, 1286, 1606, 1708, 1830, 1951
BsmBI	CGTCTC	2 cut(s)	287, 1830
BsmFI	GGGAC	2 cut(s)	464, 2001
BsmI	GAATGC	4 cut(s)	578, 896, 2341, 2588
BsnI	GGCC	8 cut(s)	20, 75, 87, 135, 753, 1223, 2685, 2943
Bso31I	GGTCTC	2 cut(s)	1018, 1951
BsoBI	CYCGRG	2 cut(s)	1229, 2450
Bsp119I	TTCGAA	1 cut(s)	1261
Bsp1286I	GDGCHC	5 cut(s)	163, 1310, 1682, 2071, 2918
Bsp13I	TCCGGA	2 cut(s)	980, 1930
Bsp1407I	TGTACA	2 cut(s)	1606, 2713
Bsp19I	CCATGG	1 cut(s)	1999
BspANI	GGCC	8 cut(s)	20, 75, 87, 135, 753, 1223, 2685, 2943
BspCNI	CTCAG	4 cut(s)	457, 1111, 1429, 2140
BspDI	ATCGAT	1 cut(s)	2199
BspEI	TCCGGA	2 cut(s)	980, 1930
BspFNI	CGCG	1 cut(s)	99
BspLI	GGNNCC	7 cut(s)	44, 119, 306, 1075, 1206, 2257, 2692
BspMAI	CTGCAG	2 cut(s)	406, 1471
BspPI	GGATC	8 cut(s)	118, 621, 1276, 1758, 1814, 2248, 2436, 2513
BspQI	GCTCTTC	3 cut(s)	209, 1298, 1385
BspT104I	TTCGAA	1 cut(s)	1261
BspT107I	GGYRCC	6 cut(s)	42, 117, 304, 1073, 1204, 2255
BspTNI	GGTCTC	2 cut(s)	1018, 1951
BsrBI	CCGCTC	1 cut(s)	236
BsrDI	GCAATG	1 cut(s)	2332
BsrFI	RCCGGY	2 cut(s)	71, 131
BsrGI	TGTACA	2 cut(s)	1606, 2713
BsrI	ACTGG	6 cut(s)	386, 657, 1018, 1136, 2504, 2794
BssAI	RCCGGY	2 cut(s)	71, 131
BssECI	CCNNGG	9 cut(s)	76, 180, 197, 308, 556, 739, 1650, 1999, 2686
BssNI	GRCGYC	1 cut(s)	2959
BssT1I	CCWWGG	3 cut(s)	308, 1999, 2686
Bst2UI	CCWGG	9 cut(s)	40, 365, 558, 741, 779, 1209, 1518, 1652, 2925
Bst4CI	ACNGT	9 cut(s)	230, 259, 536, 880, 2219, 2512, 2718, 2812, 2968
Bst6I	CTCTTC	7 cut(s)	9, 209, 1298, 1385, 1445, 1484, 1954
BstACI	GRCGYC	1 cut(s)	2959
BstAPI	GCANNNNNTGC	4 cut(s)	410, 815, 1847, 2832
BstAUI	TGTACA	2 cut(s)	1606, 2713
BstBI	TTCGAA	1 cut(s)	1261
BstDEI	CTNAG	6 cut(s)	465, 1098, 1148, 1437, 2148, 2948
BstDSI	CCRYGG	2 cut(s)	76, 1999
BstF5I	GGATG	9 cut(s)	207, 253, 749, 842, 1224, 1777, 2337, 2359, 2557
BstFNI	CGCG	1 cut(s)	99
BstH2I	RGCGCY	3 cut(s)	1517, 2549, 2679
BstHHI	GCGC	3 cut(s)	1516, 2548, 2678
BstMAI	GTCTC	8 cut(s)	34, 287, 1018, 1286, 1606, 1708, 1830, 1951
BstMCI	CGRYCG	1 cut(s)	265
BstNI	CCWGG	9 cut(s)	40, 365, 558, 741, 779, 1209, 1518, 1652, 2925
BstNSI	RCATGY	3 cut(s)	149, 1649, 2106
BstSCI	CCNGG	9 cut(s)	38, 363, 556, 739, 777, 1207, 1516, 1650, 2923
BstSFI	CTRYAG	7 cut(s)	402, 501, 1467, 1986, 2286, 2665, 2671
BstUI	CGCG	1 cut(s)	99
BstV2I	GAAGAC	2 cut(s)	46, 1338
BstX2I	RGATCY	8 cut(s)	626, 1003, 1354, 1750, 1819, 2023, 2240, 2428
BstXI	CCANNNNNNTGG	1 cut(s)	711
BstYI	RGATCY	8 cut(s)	626, 1003, 1354, 1750, 1819, 2023, 2240, 2428
Bsu15I	ATCGAT	1 cut(s)	2199
BsuI	GTATCC	2 cut(s)	554, 1927
BsuRI	GGCC	8 cut(s)	20, 75, 87, 135, 753, 1223, 2685, 2943
BsuTUI	ATCGAT	1 cut(s)	2199
BtgI	CCRYGG	2 cut(s)	76, 1999
BtgZI	GCGATG	1 cut(s)	2144
BtrI	CACGTC	1 cut(s)	142
BtsCI	GGATG	9 cut(s)	207, 253, 749, 842, 1224, 1777, 2337, 2359, 2557
BtsIMutI	CAGTG	6 cut(s)	532, 876, 1011, 2224, 2663, 2964
CaiI	CAGNNNCTG	1 cut(s)	2679
CfoI	GCGC	3 cut(s)	1516, 2548, 2678
Cfr10I	RCCGGY	2 cut(s)	71, 131
Cfr13I	GGNCC	2 cut(s)	1102, 2086
ClaI	ATCGAT	1 cut(s)	2199
CseI	GACGC	2 cut(s)	88, 2948
CsiI	ACCWGGT	2 cut(s)	363, 2923
Csp6I	GTAC	9 cut(s)	498, 599, 920, 1205, 1607, 1886, 2153, 2714, 2723
CviQI	GTAC	9 cut(s)	498, 599, 920, 1205, 1607, 1886, 2153, 2714, 2723
DdeI	CTNAG	6 cut(s)	465, 1098, 1148, 1437, 2148, 2948
DraI	TTTAAA	1 cut(s)	1053
DraIII	CACNNNGTG	1 cut(s)	2927
DrdI	GACNNNNNNGTC	1 cut(s)	146
DseDI	GACNNNNNNGTC	1 cut(s)	146
EaeI	YGGCCR	5 cut(s)	73, 85, 133, 1221, 2941
Eam1104I	CTCTTC	7 cut(s)	9, 209, 1298, 1385, 1445, 1484, 1954
EarI	CTCTTC	7 cut(s)	9, 209, 1298, 1385, 1445, 1484, 1954
EciI	GGCGGA	3 cut(s)	22, 260, 1462
Ecl136II	GAGCTC	4 cut(s)	161, 1308, 1680, 2069
Eco130I	CCWWGG	3 cut(s)	308, 1999, 2686
Eco24I	GRGCYC	5 cut(s)	163, 1310, 1682, 2071, 2918
Eco31I	GGTCTC	2 cut(s)	1018, 1951
Eco32I	GATATC	1 cut(s)	832
Eco47I	GGWCC	2 cut(s)	1102, 2086
Eco53kI	GAGCTC	4 cut(s)	161, 1308, 1680, 2069
Eco57I	CTGAAG	4 cut(s)	351, 427, 1409, 2400
Eco88I	CYCGRG	2 cut(s)	1229, 2450
EcoICRI	GAGCTC	4 cut(s)	161, 1308, 1680, 2069
EcoO109I	RGGNCCY	1 cut(s)	1102
EcoRII	CCWGG	9 cut(s)	38, 363, 556, 739, 777, 1207, 1516, 1650, 2923
EcoRV	GATATC	1 cut(s)	832
EcoT14I	CCWWGG	3 cut(s)	308, 1999, 2686
EcoT22I	ATGCAT	2 cut(s)	253, 1328
EcoT38I	GRGCYC	5 cut(s)	163, 1310, 1682, 2071, 2918
ErhI	CCWWGG	3 cut(s)	308, 1999, 2686
Esp3I	CGTCTC	2 cut(s)	287, 1830
FalI	AAGNNNNNCTT	2 cut(s)	1576, 1608
FaqI	GGGAC	2 cut(s)	464, 2001
FauI	CCCGC	3 cut(s)	638, 1843, 2756
FauNDI	CATATG	3 cut(s)	1708, 1789, 1843
FbaI	TGATCA	1 cut(s)	2491
FblI	GTMKAC	1 cut(s)	2285
FokI	GGATG	9 cut(s)	194, 260, 756, 829, 1211, 1764, 2344, 2366, 2544
FriOI	GRGCYC	5 cut(s)	163, 1310, 1682, 2071, 2918
FspBI	CTAG	4 cut(s)	309, 1880, 2175, 2553
GlaI	GCGC	3 cut(s)	1515, 2547, 2677
GsaI	CCCAGC	3 cut(s)	417, 451, 860
GsuI	CTGGAG	1 cut(s)	22
HaeII	RGCGCY	3 cut(s)	1517, 2549, 2679
HaeIII	GGCC	8 cut(s)	20, 75, 87, 135, 753, 1223, 2685, 2943
HapII	CCGG	7 cut(s)	72, 132, 156, 483, 981, 1931, 2956
HgaI	GACGC	2 cut(s)	88, 2948
HhaI	GCGC	3 cut(s)	1516, 2548, 2678
Hin1I	GRCGYC	1 cut(s)	2959
Hin6I	GCGC	3 cut(s)	1514, 2546, 2676
HinP1I	GCGC	3 cut(s)	1514, 2546, 2676
HindIII	AAGCTT	4 cut(s)	803, 1145, 1590, 2382
HpaII	CCGG	7 cut(s)	72, 132, 156, 483, 981, 1931, 2956
Hpy166II	GTNNAC	4 cut(s)	698, 1628, 1886, 2286
Hpy8I	GTNNAC	4 cut(s)	698, 1628, 1886, 2286
Hpy99I	CGWCG	4 cut(s)	26, 104, 107, 2964
HpyAV	CCTTC	8 cut(s)	640, 1061, 1115, 1155, 1456, 1789, 1928, 2038
HpyCH4III	ACNGT	9 cut(s)	230, 259, 536, 880, 2219, 2512, 2718, 2812, 2968
HpyCH4IV	ACGT	3 cut(s)	66, 141, 597
HpyF3I	CTNAG	6 cut(s)	465, 1098, 1148, 1437, 2148, 2948
HpySE526I	ACGT	3 cut(s)	66, 141, 597
Hsp92I	GRCGYC	1 cut(s)	2959
HspAI	GCGC	3 cut(s)	1514, 2546, 2676
Kpn2I	TCCGGA	2 cut(s)	980, 1930
KpnI	GGTACC	1 cut(s)	1208
KroI	GCCGGC	1 cut(s)	131
KroNI	GCCGGC	1 cut(s)	133
Ksp22I	TGATCA	1 cut(s)	2491
LguI	GCTCTTC	3 cut(s)	209, 1298, 1385
LmnI	GCTCC	9 cut(s)	158, 166, 233, 781, 1313, 1685, 2066, 2074, 2777
LweI	GCATC	9 cut(s)	238, 281, 400, 805, 851, 1145, 1162, 1566, 2721
MabI	ACCWGGT	2 cut(s)	363, 2923
MaeI	CTAG	4 cut(s)	309, 1880, 2175, 2553
MaeII	ACGT	3 cut(s)	66, 141, 597
MaeIII	GTNAC	9 cut(s)	67, 151, 433, 618, 2409, 2498, 2506, 2528, 2581
MbiI	CCGCTC	1 cut(s)	236
MfeI	CAATTG	1 cut(s)	1483
MflI	RGATCY	8 cut(s)	626, 1003, 1354, 1750, 1819, 2023, 2240, 2428
MhlI	GDGCHC	5 cut(s)	163, 1310, 1682, 2071, 2918
MlsI	TGGCCA	2 cut(s)	1223, 2943
MluNI	TGGCCA	2 cut(s)	1223, 2943
MlyI	GAGTC	4 cut(s)	36, 323, 1470, 2462
MmeI	TCCRAC	2 cut(s)	2044, 2249
Mox20I	TGGCCA	2 cut(s)	1223, 2943
Mph1103I	ATGCAT	2 cut(s)	253, 1328
MroI	TCCGGA	2 cut(s)	980, 1930
MroNI	GCCGGC	1 cut(s)	131
MroXI	GAANNNNTTC	2 cut(s)	2200, 2574
MscI	TGGCCA	2 cut(s)	1223, 2943
MseI	TTAA	5 cut(s)	1052, 1868, 2006, 2439, 2603
Msp20I	TGGCCA	2 cut(s)	1223, 2943
MspA1I	CMGCKG	2 cut(s)	447, 1472
MspI	CCGG	7 cut(s)	72, 132, 156, 483, 981, 1931, 2956
MspR9I	CCNGG	9 cut(s)	40, 365, 558, 741, 779, 1209, 1518, 1652, 2925
MunI	CAATTG	1 cut(s)	1483
Mva1269I	GAATGC	4 cut(s)	578, 896, 2341, 2588
MvaI	CCWGG	9 cut(s)	40, 365, 558, 741, 779, 1209, 1518, 1652, 2925
MvnI	CGCG	1 cut(s)	99
NaeI	GCCGGC	1 cut(s)	133
NcoI	CCATGG	1 cut(s)	1999
NdeI	CATATG	3 cut(s)	1708, 1789, 1843
NgoMIV	GCCGGC	1 cut(s)	131
NlaIV	GGNNCC	7 cut(s)	44, 119, 306, 1075, 1206, 2257, 2692
NmeAIII	GCCGAG	2 cut(s)	178, 422
NmuCI	GTSAC	5 cut(s)	67, 151, 433, 2409, 2498
NsiI	ATGCAT	2 cut(s)	253, 1328
NspI	RCATGY	3 cut(s)	149, 1649, 2106
NspV	TTCGAA	1 cut(s)	1261
PaeI	GCATGC	1 cut(s)	1649
PciSI	GCTCTTC	3 cut(s)	209, 1298, 1385
PcsI	WCGNNNNNNNCGW	4 cut(s)	18, 63, 96, 147
PctI	GAATGC	4 cut(s)	578, 896, 2341, 2588
PdiI	GCCGGC	1 cut(s)	133
PdmI	GAANNNNTTC	2 cut(s)	2200, 2574
PflFI	GACNNNGTC	1 cut(s)	26
PflMI	CCANNNNNTGG	1 cut(s)	2047
Ple19I	CGATCG	1 cut(s)	265
PleI	GAGTC	4 cut(s)	35, 322, 1470, 2461
PpsI	GAGTC	4 cut(s)	35, 322, 1470, 2461
PpuMI	RGGWCCY	1 cut(s)	1102
Psp124BI	GAGCTC	4 cut(s)	163, 1310, 1682, 2071
Psp5II	RGGWCCY	1 cut(s)	1102
Psp6I	CCWGG	9 cut(s)	38, 363, 556, 739, 777, 1207, 1516, 1650, 2923
PspFI	CCCAGC	3 cut(s)	413, 447, 856
PspGI	CCWGG	9 cut(s)	38, 363, 556, 739, 777, 1207, 1516, 1650, 2923
PspN4I	GGNNCC	7 cut(s)	44, 119, 306, 1075, 1206, 2257, 2692
PspPI	GGNCC	2 cut(s)	1102, 2086
PspPPI	RGGWCCY	1 cut(s)	1102
PsrI	GAACNNNNNNTAC	2 cut(s)	1869, 1901
PstI	CTGCAG	2 cut(s)	406, 1471
PstNI	CAGNNNCTG	1 cut(s)	2679
PsuI	RGATCY	8 cut(s)	626, 1003, 1354, 1750, 1819, 2023, 2240, 2428
PsyI	GACNNNGTC	1 cut(s)	26
PvuI	CGATCG	1 cut(s)	265
PvuII	CAGCTG	2 cut(s)	447, 1472
RsaI	GTAC	9 cut(s)	499, 600, 921, 1206, 1608, 1887, 2154, 2715, 2724
RsaNI	GTAC	9 cut(s)	498, 599, 920, 1205, 1607, 1886, 2153, 2714, 2723
SacI	GAGCTC	4 cut(s)	163, 1310, 1682, 2071
SapI	GCTCTTC	3 cut(s)	209, 1298, 1385
SaqAI	TTAA	5 cut(s)	1052, 1868, 2006, 2439, 2603
Sau96I	GGNCC	2 cut(s)	1102, 2086
SchI	GAGTC	4 cut(s)	36, 323, 1470, 2462
ScrFI	CCNGG	9 cut(s)	40, 365, 558, 741, 779, 1209, 1518, 1652, 2925
SduI	GDGCHC	5 cut(s)	163, 1310, 1682, 2071, 2918
SexAI	ACCWGGT	2 cut(s)	363, 2923
SfaNI	GCATC	9 cut(s)	238, 281, 400, 805, 851, 1145, 1162, 1566, 2721
SfcI	CTRYAG	7 cut(s)	402, 501, 1467, 1986, 2286, 2665, 2671
SfuI	TTCGAA	1 cut(s)	1261
SinI	GGWCC	2 cut(s)	1102, 2086
SmlI	CTYRAG	3 cut(s)	965, 1034, 2837
SmoI	CTYRAG	3 cut(s)	965, 1034, 2837
SphI	GCATGC	1 cut(s)	1649
SspI	AATATT	1 cut(s)	2856
SspMI	CTAG	4 cut(s)	309, 1880, 2175, 2553
SstI	GAGCTC	4 cut(s)	163, 1310, 1682, 2071
StyD4I	CCNGG	9 cut(s)	38, 363, 556, 739, 777, 1207, 1516, 1650, 2923
StyI	CCWWGG	3 cut(s)	308, 1999, 2686
TaaI	ACNGT	9 cut(s)	230, 259, 536, 880, 2219, 2512, 2718, 2812, 2968
TaiI	ACGT	3 cut(s)	69, 144, 600
TaqI	TCGA	6 cut(s)	105, 261, 1261, 1961, 2199, 2578
TatI	WGTACW	2 cut(s)	1606, 2713
TauI	GCSGC	5 cut(s)	118, 842, 1447, 1994, 2636
Tru1I	TTAA	5 cut(s)	1052, 1868, 2006, 2439, 2603
Tru9I	TTAA	5 cut(s)	1052, 1868, 2006, 2439, 2603
TscAI	CASTG	6 cut(s)	539, 883, 1018, 2224, 2663, 2971
TseFI	GTSAC	5 cut(s)	67, 151, 433, 2409, 2498
Tsp45I	GTSAC	5 cut(s)	67, 151, 433, 2409, 2498
TspGWI	ACGGA	7 cut(s)	39, 572, 1474, 1785, 1812, 2061, 2790
TspRI	CASTG	6 cut(s)	539, 883, 1018, 2224, 2663, 2971
Tth111I	GACNNNGTC	1 cut(s)	26
Van91I	CCANNNNNTGG	1 cut(s)	2047
VpaK11BI	GGWCC	2 cut(s)	1102, 2086
XapI	RAATTY	4 cut(s)	793, 2169, 2572, 2614
XceI	RCATGY	3 cut(s)	149, 1649, 2106
XmaJI	CCTAGG	1 cut(s)	308
XmiI	GTMKAC	1 cut(s)	2285
XmnI	GAANNNNTTC	2 cut(s)	2200, 2574
XspI	CTAG	4 cut(s)	309, 1880, 2175, 2553
Zsp2I	ATGCAT	2 cut(s)	253, 1328

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.