Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0006249

Physical Location & Seq

Genomic Coordinates Reverse (-)

64437337 .. 64446332

Gene Structure 8996 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ27448

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2982 bp

ATGAGGATTGAGGAAGAGGCCGACGGAGTCTCCGCGTCCAGGAACCACCTCGTCTTCGCTTACTACGTCACCGGCCACGGCTTCGGCCACGCCACTCGCGTCGTCGAGGTGGTGCGTCACCTGATTCTCGCCGGTCATGACGTCCACGTGGTCACCGGAGCTCCGGACTTTGTTTTCACGTCGGAGGTACAGTCGCCTCGACTCTTCATTCGCAAGGTGCTGCTGGACTGTGGAGCGGTTCAGGCGGATGCCTTAACAGTGGATCGCCTTGCATCTTTGGAGAAGTATTCCGAGACGGCTGTGGTGCCAAGGGAGTCTATATTGAAAACTGAAGTAGAGTGGCTGACCTCCATCAAAGCTGACCTGGTGGTCTCGGATGTTGTTCCAGTTGCGTGCCGAGCTGCAGCTGATGCTGGAATTCGCTCCGTCTGTGTCACTAACTTTAGCTGGGACTTCATCTATGCAGAATATGTCATGGCAGCCGGAAGTCATCATCGTTCAATAGTTTGGCAGATAGCAGAAGATTATTCTCACTGTGAGTTCCTGATCCGCCTCCCAGGATACTGCCCAATGCCTGCATTCCGTGATGTAATTGACGTACCTTTAGTTGTGAGGAGGTTGCACAGATCCCGCAAAGAGGTGAGAAAGGAACTAGGAATTGAAGAAGATGCCAAATTAGTCATTCTCAACTTTGGTGGACAGCCATCAGGCTGGAAGTTGAAAGAGGATTTTTTACCCCCTGGATGGCTTGGCCTGGTTTGTGGTGCTTCTGACAGCCAGGAGCTTCCACCAAATTTTAGGAAGCTTGCAAAAGATGCTTATACACCTGATATTATAGCAGCATCTGATTGTATGCTGGGAAAAATTGGATACGGCACTGTGAGTGAAGCCCTTGCATTCAAGTTGCCATTTGTCTTTGTACGCAGAGATTATTTCAATGAAGAACCTTTTTTGAGAAATATGCTTGAGCATTATCAATCTGGCGTTGAGATGATTAGGAGAGATTTGCTCACTGGTCATTGGAGACCTTACCTTGAGCGTGCAATCAGTTTAAAACCTTGCTATGAAGGAGGCATCAATGGTGGTGAGGTGGCAGCTCAGGTCCTTCAAGAAACAGCTATTGGGAAAAACTGGGCATCAGATAAGCTTAGTGGGGCAAGAAGGTTGCGTGATGCTATCATTCTTGGGTATCAACTTCAAAGGGTCCCTGGACGAGATATGGCCATTCCTGAATGGTATGCAAATGCTGAAACTGAACTTCGAATTGGATCACCAACTTGTCAAATGAGTGAGAATGACGAGCAGAGCTCCCTGATGAACTCATGCATTGAAGACTTTGAGATTCTTCATGGAGATCTTCAAGGTCTTTCCGATACAATGACTTTTTTGAAGAGCTTGGCAGAACTAGATTCTGCATATGAATCTGAAAAAGCTACTGAGAAGCGGCAGATAAGAGAGCGGAAGGCTGCAGCCGGACTCTTCAATTGGGAGGAAGAAATTTTTGTGGCAAGAGCACCTGGACGATTAGATGTAATGGGTGGGATTGCTGACTATTCAGGAAGCCTTGTCTTGCAGATGCCCATAAGAGAAGCTTGCCATGTTGCTGTACAGAGACATCAACCTAGTAAACACAGGCTCTGGAAGCATGCCCTGGCTCGGCAGGAAGCCAAAGGACAAAGCTCAACCCCTGTCTTGCAAATTGTCTCGTATGGTTCAGAGTTGAGCAATCGTAGTCCTACTTTTGACATGGATCTATCTGATTTTATGGACGGAGATCATCCAATGTCATATGAGAAAGCAAAAATATATTTTTCACAAGATCCGTCTCAAAAGTGGGCAGCATATGTTGCGGGTGTCATTCTGGTTTTAATGACAGAACTAGGTGTACGCTTTAAGGAGAGCATTAGTTTGCTGGTATCCTCTTTAGTTCCGGAAGGCAAAGGTGTTTCTTCTTCTGCTTCGGTGGAGGTTGCTACAATGTCTGCTATAGCTGCTGCCCATGGATTAAGCATCAGTCCCAGAGATCTTGCTTTGCTTTGCCAGAAGGTGGAGAATCATATTGTTGGAGCTCCGTGTGGTGTGATGGACCAAATGACTTCTGCATGTGGTGAAGCCAACAAGCTTCTTGCAATGGTGTGCCAGCCTGCTGAGGTACTTGGGCTTGTAGAAATTCCTGGCCATGTCCGATTTTGGGGAATCGATTCAGGCATAAGACACAGTGTTGGTGGTGCGGATTATGGATCTGTTAGAGTAGGTGCCTTTATGGGTCGGACGATCATAAAGTCTACAGCTTCTACGATTATGTCTCGATCATTGTCAAATGGAAATGGGATGAATCTTGACGAGTTAGAGGATGATGGTTTTGAACTGCCTAAAGCTGAAGCTTCATTAGATTATTTGTGCAACCTGTCACCTCATAGATACGATGCTCTTTATGTTAAGATACTTCCCGAGTCCATACTTGGTGAGGCTTTTCTGGAGAAGTATGCTGATCATGGTGACCCAGTTACAGTTATTGATCCAAAGTGTATGTATGGAGTGGGAGCACCTACTAGACATCCTATATATGAAAATTTCCGAGTTAAGGCATTCAAAGCACTTCTAACATCTGCAAATTCAGATGATCAGCTTACGGCTCTTGGAGAGTTATTGTATCAGTGCCACTACAGCTACAGTGCCTGTGGACTTGGCTCCGATGGAACCAATAGGCTTGTACAGTTGGTGCAAGAGATGCAGCATAGTAAATCATCTAAATTAGATGGTGGGGCATTATATGGAGCAAAGATTACAGGTGGAGGTTCTGGCGGAACAGTTTGCGCGGTTGGCAGGAATTGCCTGAAGAGCAGCCAACAAATATTTGAGATTCAGCAGAGATACAAAGCTGCAACTGGGTATTTGCCATATATTTTTGAGGGCTCATCACCAGGTGCAGGCAAATTTGGGCATCTAAGAATCCGGCGCCGCTCTGTCAAGCTGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0005975 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009506 GO:0009702 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0030054 GO:0044237 GO:0044238 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046835 GO:0055044 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

GHMP_kinases_C GHMP_kinases_N GalKase_gal_bdg Glyco_trans_1_3

Protein Analysis

993

Amino Acids

109.21

Weight (kDa)

5.95

Isoelectric Point (pI)

44.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GalKase_gal_bdg	PF10509	497 - 538	3.9e-07	Galactokinase galactose-binding signature
GHMP_kinases_N	PF00288	615 - 703	4.7e-17	GHMP kinases N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G42850 AT3G42850 AT4G16130
fragaria_vesca	FvH4_2g36460 FvH4_2g36460 FvH4_2g36460
malus_domestica	MD08G1124400.v1.1 MD15G1103100.v1.1
prunus_persica	Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1
pyrus_communis	pycom08g10310 pycom15g09400
rosa_chinensis	RchiOBHm_Chr6g0305421
rosa_laevigata	RLG00000010892
rosa_multiflora	Rmu_sc0002782.1_g000020
rosa_roxburghii	Rroxscaffold_7G00162820
rosa_rugosa	Rorug06G0340200
rosa_samantha	Rh6AG452200 Rh6BG449500 Rh6CG466000 Rh6DG452700
rosa_wichuraiana	Rw6G039400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	368
AatII	GACGTC	1 cut(s)	144
AccB1I	GGYRCC	3 cut(s)	304, 2255, 2958
AccB7I	CCANNNNNTGG	1 cut(s)	2047
AccBSI	CCGCTC	3 cut(s)	236, 1459, 2964
AccI	GTMKAC	1 cut(s)	2285
AccII	CGCG	3 cut(s)	35, 99, 2819
AccIII	TCCGGA	2 cut(s)	163, 1930
AclWI	GGATC	8 cut(s)	270, 541, 621, 1276, 1758, 1814, 2248, 2513
AcoI	YGGCCR	4 cut(s)	73, 85, 1221, 2176
AcsI	RAATTY	7 cut(s)	417, 793, 1497, 2169, 2572, 2614, 2936
AcuI	CTGAAG	3 cut(s)	351, 2400, 2858
AcvI	CACGTG	1 cut(s)	148
AcyI	GRCGYC	2 cut(s)	141, 2959
AdeI	CACNNNGTG	1 cut(s)	2927
AfaI	GTAC	7 cut(s)	189, 600, 921, 1608, 1887, 2154, 2715
AfiI	CCNNNNNNNGG	8 cut(s)	39, 637, 744, 1656, 2047, 2191, 2584, 2930
AjiI	CACGTC	1 cut(s)	180
AjuI	GAANNNNNNNTTGG	4 cut(s)	1104, 1136, 1248, 1280
Alw21I	GWGCWC	5 cut(s)	163, 1310, 1516, 2071, 2548
Alw26I	GTCTC	8 cut(s)	34, 287, 376, 1018, 1606, 1708, 1830, 2310
AlwI	GGATC	8 cut(s)	270, 541, 621, 1276, 1758, 1814, 2248, 2513
AlwNI	CAGNNNCTG	3 cut(s)	845, 1638, 2679
Ama87I	CYCGRG	1 cut(s)	2450
Aor13HI	TCCGGA	2 cut(s)	163, 1930
AoxI	GGCC	6 cut(s)	18, 73, 85, 751, 1221, 2176
ApoI	RAATTY	7 cut(s)	417, 793, 1497, 2169, 2572, 2614, 2936
ArsI	GACNNNNNNTTYG	2 cut(s)	192, 224
Asp700I	GAANNNNTTC	1 cut(s)	2200
AspLEI	GCGC	2 cut(s)	2819, 2961
AspS9I	GGNCC	3 cut(s)	1102, 1204, 2086
AsuII	TTCGAA	1 cut(s)	1261
AvaI	CYCGRG	1 cut(s)	2450
AvaII	GGWCC	3 cut(s)	1102, 1204, 2086
BalI	TGGCCA	2 cut(s)	1223, 2178
BanI	GGYRCC	3 cut(s)	304, 2255, 2958
BanII	GRGCYC	4 cut(s)	163, 1310, 2071, 2918
BbrPI	CACGTG	1 cut(s)	148
BbsI	GAAGAC	2 cut(s)	46, 1338
Bbv12I	GWGCWC	5 cut(s)	163, 1310, 1516, 2071, 2548
BbvCI	CCTCAGC	1 cut(s)	2148
BccI	CCATC	7 cut(s)	359, 712, 738, 2077, 2351, 2690, 2753
BceAI	ACGGC	4 cut(s)	94, 312, 889, 2649
BciVI	GTATCC	3 cut(s)	554, 863, 1927
BclI	TGATCA	2 cut(s)	2491, 2623
BcoDI	GTCTC	8 cut(s)	34, 287, 376, 1018, 1606, 1708, 1830, 2310
BfaI	CTAG	6 cut(s)	653, 1406, 1623, 1880, 2553, 2980
BfmI	CTRYAG	6 cut(s)	402, 1467, 1986, 2286, 2665, 2671
BfoI	RGCGCY	1 cut(s)	2962
BfuI	GTATCC	3 cut(s)	554, 863, 1927
BglII	AGATCT	2 cut(s)	1354, 2023
Bme18I	GGWCC	3 cut(s)	1102, 1204, 2086
BmeT110I	CYCGRG	1 cut(s)	2450
BmgBI	CACGTC	1 cut(s)	180
BmgT120I	GGNCC	3 cut(s)	1102, 1204, 2086
BmiI	GGNNCC	8 cut(s)	44, 306, 1205, 1206, 2257, 2692, 2701, 2960
BmrI	ACTGGG	3 cut(s)	1141, 2498, 2898
BmuI	ACTGGG	3 cut(s)	1141, 2498, 2898
BpiI	GAAGAC	2 cut(s)	46, 1338
BplI	GAGNNNNNCTC	4 cut(s)	993, 1025, 1292, 1324
BpmI	CTGGAG	1 cut(s)	2498
Bpu10I	CCTNAGC	2 cut(s)	1098, 2148
Bpu14I	TTCGAA	1 cut(s)	1261
BpuEI	CTTGAG	2 cut(s)	986, 1055
Bsa29I	ATCGAT	1 cut(s)	2199
BsaAI	YACGTR	1 cut(s)	148
BsaHI	GRCGYC	2 cut(s)	141, 2959
BsaI	GGTCTC	2 cut(s)	376, 1018
BsaJI	CCNNGG	7 cut(s)	76, 308, 556, 739, 1207, 1650, 1999
BsaWI	WCCGGW	3 cut(s)	155, 163, 1930
BsaXI	ACNNNNNCTCC	6 cut(s)	14, 44, 145, 175, 176, 206
Bsc4I	CCNNNNNNNGG	8 cut(s)	39, 637, 744, 1656, 2047, 2191, 2584, 2930
Bse118I	RCCGGY	2 cut(s)	71, 131
Bse1I	ACTGG	5 cut(s)	386, 1018, 1136, 2504, 2893
Bse3DI	GCAATG	1 cut(s)	2136
BseAI	TCCGGA	2 cut(s)	163, 1930
BseCI	ATCGAT	1 cut(s)	2199
BseDI	CCNNGG	7 cut(s)	76, 308, 556, 739, 1207, 1650, 1999
BseGI	GGATG	7 cut(s)	253, 382, 749, 1777, 2337, 2359, 2557
BseLI	CCNNNNNNNGG	8 cut(s)	39, 637, 744, 1656, 2047, 2191, 2584, 2930
BseMI	GCAATG	1 cut(s)	2136
BseMII	CTCAG	3 cut(s)	1112, 1428, 2139
BseNI	ACTGG	5 cut(s)	386, 1018, 1136, 2504, 2893
BseRI	GAGGAG	1 cut(s)	628
BseYI	CCCAGC	2 cut(s)	447, 856
BsgI	GTGCAG	1 cut(s)	2949
Bsh1236I	CGCG	3 cut(s)	35, 99, 2819
BshFI	GGCC	6 cut(s)	20, 75, 87, 753, 1223, 2178
BshNI	GGYRCC	3 cut(s)	304, 2255, 2958
BshVI	ATCGAT	1 cut(s)	2199
BsiHKAI	GWGCWC	5 cut(s)	163, 1310, 1516, 2071, 2548
BsiHKCI	CYCGRG	1 cut(s)	2450
BsiSI	CCGG	8 cut(s)	72, 132, 156, 164, 483, 1473, 1931, 2956
BslFI	GGGAC	3 cut(s)	464, 1190, 2001
BslI	CCNNNNNNNGG	8 cut(s)	39, 637, 744, 1656, 2047, 2191, 2584, 2930
BsmAI	GTCTC	8 cut(s)	34, 287, 376, 1018, 1606, 1708, 1830, 2310
BsmBI	CGTCTC	2 cut(s)	287, 1830
BsmFI	GGGAC	3 cut(s)	464, 1190, 2001
BsmI	GAATGC	3 cut(s)	578, 896, 2588
BsnI	GGCC	6 cut(s)	20, 75, 87, 753, 1223, 2178
Bso31I	GGTCTC	2 cut(s)	376, 1018
BsoBI	CYCGRG	1 cut(s)	2450
Bsp119I	TTCGAA	1 cut(s)	1261
Bsp1286I	GDGCHC	6 cut(s)	163, 1310, 1516, 2071, 2548, 2918
Bsp13I	TCCGGA	2 cut(s)	163, 1930
Bsp1407I	TGTACA	2 cut(s)	1606, 2713
Bsp19I	CCATGG	1 cut(s)	1999
BspANI	GGCC	6 cut(s)	20, 75, 87, 753, 1223, 2178
BspCNI	CTCAG	3 cut(s)	1111, 1429, 2140
BspDI	ATCGAT	1 cut(s)	2199
BspEI	TCCGGA	2 cut(s)	163, 1930
BspFNI	CGCG	3 cut(s)	35, 99, 2819
BspHI	TCATGA	1 cut(s)	136
BspLI	GGNNCC	8 cut(s)	44, 306, 1205, 1206, 2257, 2692, 2701, 2960
BspMAI	CTGCAG	2 cut(s)	406, 1471
BspPI	GGATC	8 cut(s)	270, 541, 621, 1276, 1758, 1814, 2248, 2513
BspQI	GCTCTTC	2 cut(s)	1385, 2834
BspT104I	TTCGAA	1 cut(s)	1261
BspT107I	GGYRCC	3 cut(s)	304, 2255, 2958
BspTNI	GGTCTC	2 cut(s)	376, 1018
BsrBI	CCGCTC	3 cut(s)	236, 1459, 2964
BsrDI	GCAATG	1 cut(s)	2136
BsrFI	RCCGGY	2 cut(s)	71, 131
BsrGI	TGTACA	2 cut(s)	1606, 2713
BsrI	ACTGG	5 cut(s)	386, 1018, 1136, 2504, 2893
BssAI	RCCGGY	2 cut(s)	71, 131
BssECI	CCNNGG	7 cut(s)	76, 308, 556, 739, 1207, 1650, 1999
BssNI	GRCGYC	2 cut(s)	141, 2959
BssT1I	CCWWGG	2 cut(s)	308, 1999
Bst6I	CTCTTC	5 cut(s)	9, 209, 1385, 1484, 2834
BstACI	GRCGYC	2 cut(s)	141, 2959
BstAPI	GCANNNNNTGC	5 cut(s)	410, 815, 1847, 2731, 2832
BstAUI	TGTACA	2 cut(s)	1606, 2713
BstBAI	YACGTR	1 cut(s)	148
BstBI	TTCGAA	1 cut(s)	1261
BstC8I	GCNNGC	9 cut(s)	394, 576, 807, 1041, 1594, 1647, 2141, 2145, 2932
BstDEI	CTNAG	5 cut(s)	1098, 1148, 1437, 2148, 2948
BstDSI	CCRYGG	2 cut(s)	76, 1999
BstEII	GGTNACC	2 cut(s)	151, 2498
BstF5I	GGATG	7 cut(s)	253, 382, 749, 1777, 2337, 2359, 2557
BstFNI	CGCG	3 cut(s)	35, 99, 2819
BstH2I	RGCGCY	1 cut(s)	2962
BstHHI	GCGC	2 cut(s)	2819, 2961
BstMAI	GTCTC	8 cut(s)	34, 287, 376, 1018, 1606, 1708, 1830, 2310
BstNSI	RCATGY	2 cut(s)	1649, 2106
BstPI	GGTNACC	2 cut(s)	151, 2498
BstSFI	CTRYAG	6 cut(s)	402, 1467, 1986, 2286, 2665, 2671
BstUI	CGCG	3 cut(s)	35, 99, 2819
BstV2I	GAAGAC	2 cut(s)	46, 1338
BstX2I	RGATCY	6 cut(s)	626, 1354, 1750, 1819, 2023, 2240
BstXI	CCANNNNNNTGG	1 cut(s)	711
BstYI	RGATCY	6 cut(s)	626, 1354, 1750, 1819, 2023, 2240
Bsu15I	ATCGAT	1 cut(s)	2199
BsuI	GTATCC	3 cut(s)	554, 863, 1927
BsuRI	GGCC	6 cut(s)	20, 75, 87, 753, 1223, 2178
BsuTUI	ATCGAT	1 cut(s)	2199
BtgI	CCRYGG	2 cut(s)	76, 1999
BtrI	CACGTC	1 cut(s)	180
BtsCI	GGATG	7 cut(s)	253, 382, 749, 1777, 2337, 2359, 2557
BtsIMutI	CAGTG	7 cut(s)	264, 532, 876, 1011, 2224, 2663, 2680
Cac8I	GCNNGC	9 cut(s)	394, 576, 807, 1041, 1594, 1647, 2141, 2145, 2932
CaiI	CAGNNNCTG	3 cut(s)	845, 1638, 2679
CciI	TCATGA	1 cut(s)	136
CfoI	GCGC	2 cut(s)	2819, 2961
Cfr10I	RCCGGY	2 cut(s)	71, 131
Cfr13I	GGNCC	3 cut(s)	1102, 1204, 2086
ClaI	ATCGAT	1 cut(s)	2199
CseI	GACGC	3 cut(s)	24, 88, 104
CsiI	ACCWGGT	2 cut(s)	363, 2923
Csp6I	GTAC	7 cut(s)	188, 599, 920, 1607, 1886, 2153, 2714
CspCI	CAANNNNNGTGG	2 cut(s)	676, 711
CviQI	GTAC	7 cut(s)	188, 599, 920, 1607, 1886, 2153, 2714
DdeI	CTNAG	5 cut(s)	1098, 1148, 1437, 2148, 2948
DinI	GGCGCC	1 cut(s)	2960
DraI	TTTAAA	1 cut(s)	1053
DraIII	CACNNNGTG	1 cut(s)	2927
DrdI	GACNNNNNNGTC	1 cut(s)	368
DseDI	GACNNNNNNGTC	1 cut(s)	368
EaeI	YGGCCR	4 cut(s)	73, 85, 1221, 2176
Eam1104I	CTCTTC	5 cut(s)	9, 209, 1385, 1484, 2834
EarI	CTCTTC	5 cut(s)	9, 209, 1385, 1484, 2834
EciI	GGCGGA	3 cut(s)	260, 539, 2820
Ecl136II	GAGCTC	3 cut(s)	161, 1308, 2069
Eco130I	CCWWGG	2 cut(s)	308, 1999
Eco24I	GRGCYC	4 cut(s)	163, 1310, 2071, 2918
Eco31I	GGTCTC	2 cut(s)	376, 1018
Eco47I	GGWCC	3 cut(s)	1102, 1204, 2086
Eco53kI	GAGCTC	3 cut(s)	161, 1308, 2069
Eco57I	CTGAAG	3 cut(s)	351, 2400, 2858
Eco72I	CACGTG	1 cut(s)	148
Eco88I	CYCGRG	1 cut(s)	2450
Eco91I	GGTNACC	2 cut(s)	151, 2498
EcoICRI	GAGCTC	3 cut(s)	161, 1308, 2069
EcoO109I	RGGNCCY	2 cut(s)	1102, 1204
EcoO65I	GGTNACC	2 cut(s)	151, 2498
EcoRI	GAATTC	1 cut(s)	417
EcoT14I	CCWWGG	2 cut(s)	308, 1999
EcoT22I	ATGCAT	1 cut(s)	1328
EcoT38I	GRGCYC	4 cut(s)	163, 1310, 2071, 2918
EgeI	GGCGCC	1 cut(s)	2960
EheI	GGCGCC	1 cut(s)	2960
ErhI	CCWWGG	2 cut(s)	308, 1999
Esp3I	CGTCTC	2 cut(s)	287, 1830
FalI	AAGNNNNNCTT	2 cut(s)	1576, 1608
FaqI	GGGAC	3 cut(s)	464, 1190, 2001
FauI	CCCGC	2 cut(s)	638, 1843
FauNDI	CATATG	3 cut(s)	1417, 1789, 1843
FbaI	TGATCA	2 cut(s)	2491, 2623
FblI	GTMKAC	1 cut(s)	2285
FokI	GGATG	7 cut(s)	260, 389, 756, 1764, 2344, 2366, 2544
FriOI	GRGCYC	4 cut(s)	163, 1310, 2071, 2918
FspBI	CTAG	6 cut(s)	653, 1406, 1623, 1880, 2553, 2980
GlaI	GCGC	2 cut(s)	2818, 2960
GsaI	CCCAGC	2 cut(s)	451, 860
GsuI	CTGGAG	1 cut(s)	2498
HaeII	RGCGCY	1 cut(s)	2962
HaeIII	GGCC	6 cut(s)	20, 75, 87, 753, 1223, 2178
HapII	CCGG	8 cut(s)	72, 132, 156, 164, 483, 1473, 1931, 2956
HgaI	GACGC	3 cut(s)	24, 88, 104
HhaI	GCGC	2 cut(s)	2819, 2961
Hin1I	GRCGYC	2 cut(s)	141, 2959
Hin6I	GCGC	2 cut(s)	2817, 2959
HinP1I	GCGC	2 cut(s)	2817, 2959
HindIII	AAGCTT	5 cut(s)	803, 1145, 1590, 2120, 2382
HpaII	CCGG	8 cut(s)	72, 132, 156, 164, 483, 1473, 1931, 2956
Hpy166II	GTNNAC	6 cut(s)	145, 698, 1628, 1886, 2286, 2684
Hpy8I	GTNNAC	6 cut(s)	145, 698, 1628, 1886, 2286, 2684
Hpy99I	CGWCG	4 cut(s)	26, 104, 107, 184
HpyAV	CCTTC	6 cut(s)	1061, 1115, 1155, 1456, 1928, 2038
HpyCH4IV	ACGT	5 cut(s)	66, 141, 147, 179, 597
HpyF3I	CTNAG	5 cut(s)	1098, 1148, 1437, 2148, 2948
HpySE526I	ACGT	5 cut(s)	66, 141, 147, 179, 597
Hsp92I	GRCGYC	2 cut(s)	141, 2959
HspAI	GCGC	2 cut(s)	2817, 2959
KasI	GGCGCC	1 cut(s)	2958
KflI	GGGWCCC	1 cut(s)	1204
Kpn2I	TCCGGA	2 cut(s)	163, 1930
Ksp22I	TGATCA	2 cut(s)	2491, 2623
LguI	GCTCTTC	2 cut(s)	1385, 2834
MabI	ACCWGGT	2 cut(s)	363, 2923
MaeI	CTAG	6 cut(s)	653, 1406, 1623, 1880, 2553, 2980
MaeII	ACGT	5 cut(s)	66, 141, 147, 179, 597
MaeIII	GTNAC	7 cut(s)	67, 116, 151, 433, 2409, 2498, 2506
MbiI	CCGCTC	3 cut(s)	236, 1459, 2964
MfeI	CAATTG	1 cut(s)	1483
MflI	RGATCY	6 cut(s)	626, 1354, 1750, 1819, 2023, 2240
MhlI	GDGCHC	6 cut(s)	163, 1310, 1516, 2071, 2548, 2918
MlsI	TGGCCA	2 cut(s)	1223, 2178
MluNI	TGGCCA	2 cut(s)	1223, 2178
Mly113I	GGCGCC	1 cut(s)	2959
MlyI	GAGTC	5 cut(s)	36, 195, 323, 1470, 2462
MmeI	TCCRAC	3 cut(s)	162, 2044, 2249
Mox20I	TGGCCA	2 cut(s)	1223, 2178
Mph1103I	ATGCAT	1 cut(s)	1328
MroI	TCCGGA	2 cut(s)	163, 1930
MroXI	GAANNNNTTC	1 cut(s)	2200
MscI	TGGCCA	2 cut(s)	1223, 2178
MseI	TTAA	7 cut(s)	254, 1052, 1868, 1893, 2006, 2439, 2583
Msp20I	TGGCCA	2 cut(s)	1223, 2178
MspA1I	CMGCKG	1 cut(s)	407
MspI	CCGG	8 cut(s)	72, 132, 156, 164, 483, 1473, 1931, 2956
MunI	CAATTG	1 cut(s)	1483
Mva1269I	GAATGC	3 cut(s)	578, 896, 2588
MvnI	CGCG	3 cut(s)	35, 99, 2819
NarI	GGCGCC	1 cut(s)	2959
NcoI	CCATGG	1 cut(s)	1999
NdeI	CATATG	3 cut(s)	1417, 1789, 1843
NlaIV	GGNNCC	8 cut(s)	44, 306, 1205, 1206, 2257, 2692, 2701, 2960
NmeAIII	GCCGAG	2 cut(s)	422, 1636
NmuCI	GTSAC	6 cut(s)	67, 116, 151, 433, 2409, 2498
NsiI	ATGCAT	1 cut(s)	1328
NspI	RCATGY	2 cut(s)	1649, 2106
NspV	TTCGAA	1 cut(s)	1261
PaeI	GCATGC	1 cut(s)	1649
PagI	TCATGA	1 cut(s)	136
PciSI	GCTCTTC	2 cut(s)	1385, 2834
PcsI	WCGNNNNNNNCGW	2 cut(s)	63, 96
PctI	GAATGC	3 cut(s)	578, 896, 2588
PdmI	GAANNNNTTC	1 cut(s)	2200
PflFI	GACNNNGTC	1 cut(s)	26
PflMI	CCANNNNNTGG	1 cut(s)	2047
PfoI	TCCNGGA	1 cut(s)	38
PleI	GAGTC	5 cut(s)	35, 195, 322, 1470, 2461
PluTI	GGCGCC	1 cut(s)	2962
PmaCI	CACGTG	1 cut(s)	148
PmlI	CACGTG	1 cut(s)	148
PpsI	GAGTC	5 cut(s)	35, 195, 322, 1470, 2461
Ppu21I	YACGTR	1 cut(s)	148
PpuMI	RGGWCCY	2 cut(s)	1102, 1204
Psp124BI	GAGCTC	3 cut(s)	163, 1310, 2071
Psp5II	RGGWCCY	2 cut(s)	1102, 1204
PspCI	CACGTG	1 cut(s)	148
PspEI	GGTNACC	2 cut(s)	151, 2498
PspFI	CCCAGC	2 cut(s)	447, 856
PspN4I	GGNNCC	8 cut(s)	44, 306, 1205, 1206, 2257, 2692, 2701, 2960
PspPI	GGNCC	3 cut(s)	1102, 1204, 2086
PspPPI	RGGWCCY	2 cut(s)	1102, 1204
PsrI	GAACNNNNNNTAC	2 cut(s)	1869, 1901
PstI	CTGCAG	2 cut(s)	406, 1471
PstNI	CAGNNNCTG	3 cut(s)	845, 1638, 2679
PsuI	RGATCY	6 cut(s)	626, 1354, 1750, 1819, 2023, 2240
PsyI	GACNNNGTC	1 cut(s)	26
PvuII	CAGCTG	1 cut(s)	407
RsaI	GTAC	7 cut(s)	189, 600, 921, 1608, 1887, 2154, 2715
RsaNI	GTAC	7 cut(s)	188, 599, 920, 1607, 1886, 2153, 2714
SacI	GAGCTC	3 cut(s)	163, 1310, 2071
SapI	GCTCTTC	2 cut(s)	1385, 2834
SaqAI	TTAA	7 cut(s)	254, 1052, 1868, 1893, 2006, 2439, 2583
Sau96I	GGNCC	3 cut(s)	1102, 1204, 2086
SchI	GAGTC	5 cut(s)	36, 195, 323, 1470, 2462
SduI	GDGCHC	6 cut(s)	163, 1310, 1516, 2071, 2548, 2918
SexAI	ACCWGGT	2 cut(s)	363, 2923
SfcI	CTRYAG	6 cut(s)	402, 1467, 1986, 2286, 2665, 2671
SfoI	GGCGCC	1 cut(s)	2960
SfuI	TTCGAA	1 cut(s)	1261
SinI	GGWCC	3 cut(s)	1102, 1204, 2086
SmlI	CTYRAG	2 cut(s)	965, 1034
SmoI	CTYRAG	2 cut(s)	965, 1034
SphI	GCATGC	1 cut(s)	1649
SspDI	GGCGCC	1 cut(s)	2958
SspI	AATATT	1 cut(s)	2856
SspMI	CTAG	6 cut(s)	653, 1406, 1623, 1880, 2553, 2980
SstI	GAGCTC	3 cut(s)	163, 1310, 2071
StyI	CCWWGG	2 cut(s)	308, 1999
TaiI	ACGT	5 cut(s)	69, 144, 150, 182, 600
TaqI	TCGA	5 cut(s)	105, 199, 1261, 2199, 2308
TatI	WGTACW	2 cut(s)	1606, 2713
TauI	GCSGC	2 cut(s)	1447, 2964
Tru1I	TTAA	7 cut(s)	254, 1052, 1868, 1893, 2006, 2439, 2583
Tru9I	TTAA	7 cut(s)	254, 1052, 1868, 1893, 2006, 2439, 2583
TscAI	CASTG	7 cut(s)	264, 539, 883, 1018, 2224, 2663, 2680
TseFI	GTSAC	6 cut(s)	67, 116, 151, 433, 2409, 2498
Tsp45I	GTSAC	6 cut(s)	67, 116, 151, 433, 2409, 2498
TspGWI	ACGGA	6 cut(s)	39, 415, 572, 1785, 1812, 2061
TspRI	CASTG	7 cut(s)	264, 539, 883, 1018, 2224, 2663, 2680
Tth111I	GACNNNGTC	1 cut(s)	26
Van91I	CCANNNNNTGG	1 cut(s)	2047
VpaK11BI	GGWCC	3 cut(s)	1102, 1204, 2086
XapI	RAATTY	7 cut(s)	417, 793, 1497, 2169, 2572, 2614, 2936
XceI	RCATGY	2 cut(s)	1649, 2106
XmiI	GTMKAC	1 cut(s)	2285
XmnI	GAANNNNTTC	1 cut(s)	2200
XspI	CTAG	6 cut(s)	653, 1406, 1623, 1880, 2553, 2980
ZraI	GACGTC	1 cut(s)	142
Zsp2I	ATGCAT	1 cut(s)	1328

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.