Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006249

Physical Location & Seq

Genomic Coordinates Forward (+)

7281842 .. 7289211

Gene Structure 7370 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1103100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2970 bp

ATGAGGATTGACGAAGAGATCGAAGGAGTGTCGGCGTCGAGGAAACACCTGGTTTTCGCTTACTACGTCACTGGCCACGGCTTCGGCCACGCCACTCGCGTCGTCGAGGTTGCGCGGCACCTGATTCTCGCCGGCCACGATGTCCACGTCGTCACCGGAGCTCCAGATTTCGTCTTCACTTCCGAGATTCAATCGCATCGCCTCTTCATTCGAAAGGTTCTTCTGGACTGTGGAGCTGTTCAGGCAGATGCTTTAACAGTTGATCGTCTTGCTTCCTTGGCGAAGTATTTGGAGACGGCTGTGGCACCTCGGGCTTCAATCTTGAAAACGGAAGTGCAGTGGCTCACCTCCATCAAAGCTGATTTAGTGGTCTCTGATGTTGTTCCAGTTGCGTGCCGTGCAGCGGCTGATGCAGGAATTCGATCTGTTTGTGTCACCAACTTCAGTTGGGACTTTATCTACGCAGAGTATGTTATGGCTGCTGGAGATAATCATCGTTCAATCGTTTGGCAGATAGCAGAAGATTATTCCCACTGTGAGTTCCTCATCCGCCTCCCAGGATACTGCCCAATGCCTGCTTTCCGTGATGTAGTTGATGTACCTCTAGTTGTGAGGAGGTTTCGCAGATCCCGCAAAGAGGTGAGGGAGGAACTTGGAATTGGAGACGATGTAACTATAGTCATCCTCAACTTCGGTGGACAGCCAGCAGGTTGGAAGTTGAAGGAAGAGTTTTTACCCCCTGGATGGCTGTGCCTGGTTTGTGGTGGTTCTGACACCCAGGAACTTCCGTCAAATTTCATAAAGCTTGCGAAAGATGCTTATACACCTGATTTTATGGCAGCATCTGACTGTATGCTTGGAAAAATTGGATATGGCACTGTGAGTGAAGCTCTTGCGTACAAGTTACCTTTTGTCTTTGTACGCAGAGATTATTTCAATGAAGAACCTTTCTTGAGAAATATGCTTGAGTATTATCAAGGTGGAGTTGAGATGATTAGGAGGGACTTGCTCACTGGTCACTGGAAACCTTACCTTGAGCGTGCAGTCAGTTTGAAGCCATGCTATGAAGGAGGTATCAATGGTGGTGAGGTGGCAGCTCACATCCTGCAGGAGACTGCTATTGGGAAAAACTATGCATCAGATAAGCTAAGTGGGGCAAGAAGATTGCGTGATGCCATAATTCTTGGGTATCAACTACAAAGGGTACCTGGACGAGATATGGCAATTCCTGAATGGTATGCAAATGCGGAAAGTGAACTTGGAATTGGATCACCTACTTGCGAAGTGAGCGAGAAGAGCTCCCTAATGAACTCATGCACTGAAGACTTCGAGATTCTTCATGGAGATCTTCATGGTATTTCAGATACAATGACTTTTTTGAAGAGCTTGGCAGAACTAGATTCTGTTTATTACTCTGAAAAAACTACTGAGAAGCGCCGGGAGTGCAAGGCTGCAGCTGGACTCTTCAACTGGGAGGAAGAAATTTTTGTGGCAAGAGCACCTGGACGGTTGGACGTAATGGGAGGGATTGCTGACTATTCAGGAAGCCTGGTCTTGCAGATGCCGATAAAGGAAGCCTGCCATGTTGCTATACAACAAAATCATCCAAGTAAACATAGGCTCTGGAAACATGTCCTGGCCCGGCAGCAGGCAGAAGGACGAAACCCAACACCTGTCCTGCAAATTGTCTCATATGGTTCAGAGTTAAGCAACCGTAGCCCAACGTTTGACATGGATCTTTCTGATTTTATGGACGGAGATAAACCAATGTCATATGAGAAGGCAAAGAAATACTTTTCACAAGATCCATCCCAAAAGTGGGCAGCATATGTTGCAGGTGTCATTCTGGTTTTGATGATAGAACTAGGTGTACGTTTTGAGGACAGCATCAGTTTGCTGGTTTCCTCCTCAGTCCCAGAAGGCAAAGGTGTCTCTTCTTCTGCTTCTGTGGAAGTCGCTACCATGTCTGCCATAGCTGCTGCCCATGGATTAAGCATCAGTCCCAGAGATCTTGCGTTGCTTTGCCAAAAGGTGGAGAATCATATTGTTGGAGCACCATGTGGTGTGATGGACCAGATGACATCTGCATGTGGTGAAGCAAACAAGCTTCTTGCGATGGTGTGCCAGCCTGCTGAGGTTCTTGGACTTGTAGAAATTCCTGGCCATGTTTGCTTTTGGGGAATTGATTCAGGAATAAGACACAGTGTTGGAGGTGCGGATTATGGATCTGTTAGAATAGGTGCCTTTATGGGTCGGAAGATGATAAAGTGCGCAGCATCTATGATTTTGTCTCGATCATCATCAACTGCATATGGGACAATCGATGAGTTGGAGGATGATGGTTATGAACTACTTGAAGCTGAGGCTTCGTTAAATTACTTGTGCAACCTGCCACCTCATAGATATGAGGCTCTTTATGTTAAGGTCCTTCCTGAAACTATACTTGGTGAAACTTTTCTTGAGAAGTATGATGATCACAACGATCCAGTTACAGTAATTGATCCAAATTGTAGCTACGTAGTGAGATCACCTGCTAGACATCCTATATATGAAAATTTCCGGGTTAAGACCTTCAAAGCACTGCTAACATCTGCAAATTCAGATGATCAGCTTACAGCTCTTGGAGAATTACTGTATCAGTGTCACTACAGCTATAGTGCTTGCGGACTTGGCTCCGATGGAACAAATCGGCTTGTACGGTTGGTACAAGAGATGCAATATGCTAAATCATCTAAATCCGACGATGGGACGTTGTATGGAGCCAAGATTACAGGTGGAGGTTCCGGTGGAACGGTTTGTGTTGTCGGAAGGAACTGTCTGCAGAGCAGCCAACAAATTTTGGAGATTCAGCAGAGGTACAAAGATGCAACAGGGTATCTGCCATTTATTTTCGAGGGCTCGTCGCCTGGTGCCGGCAAGTTCGGGTATCTAAGAATACGCCGCCGTGTCTCCCTCGATCCAAATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0005975 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009506 GO:0009702 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0030054 GO:0044237 GO:0044238 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046835 GO:0055044 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

GHMP_kinases_C GHMP_kinases_N GalKase_gal_bdg Glyco_trans_1_3

Protein Analysis

990

Amino Acids

108.99

Weight (kDa)

5.62

Isoelectric Point (pI)

44.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GalKase_gal_bdg	PF10509	492 - 532	7.6e-07	Galactokinase galactose-binding signature
GHMP_kinases_N	PF00288	610 - 698	2.4e-17	GHMP kinases N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G42850 AT3G42850 AT4G16130
fragaria_vesca	FvH4_2g36460 FvH4_2g36460 FvH4_2g36460
malus_domestica	MD08G1124400.v1.1 MD15G1103100.v1.1
prunus_persica	Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1
pyrus_communis	pycom08g10310 pycom15g09400
rosa_chinensis	RchiOBHm_Chr6g0305421
rosa_laevigata	RLG00000010892
rosa_multiflora	Rmu_sc0002782.1_g000020
rosa_roxburghii	Rroxscaffold_7G00162820
rosa_rugosa	Rorug06G0340200
rosa_samantha	Rh6AG452200 Rh6BG449500 Rh6CG466000 Rh6DG452700
rosa_wichuraiana	Rw6G039400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1826, 2539
Acc16I	TGCGCA	1 cut(s)	2272
Acc36I	ACCTGC	4 cut(s)	698, 1826, 2397, 2539
Acc65I	GGTACC	1 cut(s)	1204
AccB1I	GGYRCC	5 cut(s)	117, 304, 1204, 2240, 2909
AccB7I	CCANNNNNTGG	1 cut(s)	2032
AccII	CGCG	2 cut(s)	99, 115
AciI	CCGC	8 cut(s)	115, 404, 550, 631, 1247, 2216, 2664, 2941
AclI	AACGTT	1 cut(s)	1724
AclWI	GGATC	8 cut(s)	621, 1276, 1743, 1799, 2233, 2477, 2495, 2951
AcoI	YGGCCR	4 cut(s)	73, 85, 133, 2161
AcsI	RAATTY	7 cut(s)	417, 793, 1482, 2154, 2554, 2596, 2835
AcuI	CTGAAG	2 cut(s)	427, 1341
AcyI	GRCGYC	1 cut(s)	35
AdeI	CACNNNGTG	1 cut(s)	2060
AfaI	GTAC	8 cut(s)	600, 899, 921, 1206, 1872, 2697, 2706, 2858
AfiI	CCNNNNNNNGG	9 cut(s)	403, 637, 744, 1648, 1818, 1819, 2032, 2745, 2912
AflIII	ACRYGT	1 cut(s)	1630
AjiI	CACGTC	1 cut(s)	148
AjuI	GAANNNNNNNTTGG	8 cut(s)	642, 674, 1242, 1248, 1274, 1280, 2427, 2459
Alw21I	GWGCWC	4 cut(s)	163, 1301, 1501, 2056
Alw26I	GTCTC	8 cut(s)	287, 376, 657, 1106, 1693, 1936, 2295, 2953
AlwI	GGATC	8 cut(s)	621, 1276, 1743, 1799, 2233, 2477, 2495, 2951
AlwNI	CAGNNNCTG	2 cut(s)	407, 845
Ama87I	CYCGRG	1 cut(s)	309
AoxI	GGCC	5 cut(s)	73, 85, 133, 1638, 2161
ApoI	RAATTY	7 cut(s)	417, 793, 1482, 2154, 2554, 2596, 2835
Asp700I	GAANNNNTTC	2 cut(s)	2185, 2454
Asp718I	GGTACC	1 cut(s)	1204
AspLEI	GCGC	3 cut(s)	115, 1437, 2273
AspS9I	GGNCC	3 cut(s)	1639, 2071, 2425
AsuC2I	CCSGG	3 cut(s)	1439, 1642, 2561
AsuHPI	GGTGA	9 cut(s)	145, 337, 427, 652, 1097, 1263, 2105, 2459, 2520
AsuII	TTCGAA	1 cut(s)	211
AvaI	CYCGRG	1 cut(s)	309
AvaII	GGWCC	2 cut(s)	2071, 2425
BalI	TGGCCA	2 cut(s)	75, 2163
BanI	GGYRCC	5 cut(s)	117, 304, 1204, 2240, 2909
BanII	GRGCYC	3 cut(s)	163, 1301, 2900
BarI	GAAGNNNNNNTAC	2 cut(s)	717, 749
BbsI	GAAGAC	2 cut(s)	166, 1329
Bbv12I	GWGCWC	4 cut(s)	163, 1301, 1501, 2056
BbvCI	CCTCAGC	2 cut(s)	2133, 2361
BccI	CCATC	8 cut(s)	359, 738, 1816, 2062, 2110, 2333, 2672, 2738
BceAI	ACGGC	4 cut(s)	94, 312, 381, 2928
BciVI	GTATCC	1 cut(s)	554
BclI	TGATCA	2 cut(s)	2473, 2605
BcnI	CCSGG	3 cut(s)	1439, 1642, 2561
BcoDI	GTCTC	8 cut(s)	287, 376, 657, 1106, 1693, 1936, 2295, 2953
BfaI	CTAG	4 cut(s)	605, 1397, 1865, 2535
BfmI	CTRYAG	6 cut(s)	675, 1106, 1452, 2647, 2653, 2819
BfoI	RGCGCY	1 cut(s)	1438
BfuAI	ACCTGC	4 cut(s)	698, 1826, 2397, 2539
BfuI	GTATCC	1 cut(s)	554
BglII	AGATCT	2 cut(s)	1345, 2008
Bme18I	GGWCC	2 cut(s)	2071, 2425
BmeT110I	CYCGRG	1 cut(s)	309
BmgBI	CACGTC	1 cut(s)	148
BmgT120I	GGNCC	3 cut(s)	1639, 2071, 2425
BmiI	GGNNCC	8 cut(s)	119, 306, 1206, 2242, 2674, 2761, 2782, 2911
BmrI	ACTGGG	1 cut(s)	1480
BmuI	ACTGGG	1 cut(s)	1480
BpiI	GAAGAC	2 cut(s)	166, 1329
BplI	GAGNNNNNCTC	4 cut(s)	874, 906, 1283, 1315
BpmI	CTGGAG	2 cut(s)	147, 504
Bpu10I	CCTNAGC	2 cut(s)	2133, 2361
Bpu14I	TTCGAA	1 cut(s)	211
BpuEI	CTTGAG	4 cut(s)	973, 986, 1055, 2480
BpuMI	CCSGG	3 cut(s)	1439, 1642, 2561
Bsa29I	ATCGAT	1 cut(s)	2322
BsaAI	YACGTR	1 cut(s)	2518
BsaBI	GATNNNNATC	2 cut(s)	492, 2299
BsaHI	GRCGYC	1 cut(s)	35
BsaI	GGTCTC	1 cut(s)	376
BsaJI	CCNNGG	7 cut(s)	76, 276, 308, 556, 739, 777, 1984
BsaWI	WCCGGW	2 cut(s)	155, 2783
BsaXI	ACNNNNNCTCC	4 cut(s)	145, 175, 654, 684
Bsc4I	CCNNNNNNNGG	9 cut(s)	403, 637, 744, 1648, 1818, 1819, 2032, 2745, 2912
Bse118I	RCCGGY	2 cut(s)	131, 2912
Bse1I	ACTGG	6 cut(s)	76, 386, 1018, 1025, 1475, 2486
Bse8I	GATNNNNATC	2 cut(s)	492, 2299
BseCI	ATCGAT	1 cut(s)	2322
BseDI	CCNNGG	7 cut(s)	76, 276, 308, 556, 739, 777, 1984
BseGI	GGATG	8 cut(s)	546, 681, 749, 1101, 1603, 1808, 2341, 2539
BseJI	GATNNNNATC	2 cut(s)	492, 2299
BseLI	CCNNNNNNNGG	9 cut(s)	403, 637, 744, 1648, 1818, 1819, 2032, 2745, 2912
BseMII	CTCAG	4 cut(s)	1419, 1923, 2124, 2352
BseNI	ACTGG	6 cut(s)	76, 386, 1018, 1025, 1475, 2486
BseRI	GAGGAG	2 cut(s)	628, 1897
BsgI	GTGCAG	3 cut(s)	356, 420, 1062
Bsh1236I	CGCG	2 cut(s)	99, 115
BshFI	GGCC	5 cut(s)	75, 87, 135, 1640, 2163
BshNI	GGYRCC	5 cut(s)	117, 304, 1204, 2240, 2909
BshVI	ATCGAT	1 cut(s)	2322
BsiHKAI	GWGCWC	4 cut(s)	163, 1301, 1501, 2056
BsiHKCI	CYCGRG	1 cut(s)	309
BsiSI	CCGG	7 cut(s)	132, 156, 1438, 1642, 2560, 2784, 2913
BslFI	GGGAC	6 cut(s)	464, 1016, 1898, 1986, 2329, 2761
BslI	CCNNNNNNNGG	9 cut(s)	403, 637, 744, 1648, 1818, 1819, 2032, 2745, 2912
BsmAI	GTCTC	8 cut(s)	287, 376, 657, 1106, 1693, 1936, 2295, 2953
BsmBI	CGTCTC	2 cut(s)	287, 657
BsmFI	GGGAC	6 cut(s)	464, 1016, 1898, 1986, 2329, 2761
BsnI	GGCC	5 cut(s)	75, 87, 135, 1640, 2163
Bso31I	GGTCTC	1 cut(s)	376
BsoBI	CYCGRG	1 cut(s)	309
Bsp119I	TTCGAA	1 cut(s)	211
Bsp1286I	GDGCHC	5 cut(s)	163, 1301, 1501, 2056, 2900
Bsp19I	CCATGG	1 cut(s)	1984
BspACI	CCGC	8 cut(s)	115, 404, 550, 631, 1247, 2216, 2664, 2941
BspANI	GGCC	5 cut(s)	75, 87, 135, 1640, 2163
BspCNI	CTCAG	4 cut(s)	1420, 1922, 2125, 2353
BspDI	ATCGAT	1 cut(s)	2322
BspFNI	CGCG	2 cut(s)	99, 115
BspLI	GGNNCC	8 cut(s)	119, 306, 1206, 2242, 2674, 2761, 2782, 2911
BspMAI	CTGCAG	3 cut(s)	1110, 1456, 2823
BspMI	ACCTGC	4 cut(s)	698, 1826, 2397, 2539
BspPI	GGATC	8 cut(s)	621, 1276, 1743, 1799, 2233, 2477, 2495, 2951
BspQI	GCTCTTC	2 cut(s)	1289, 1376
BspT104I	TTCGAA	1 cut(s)	211
BspT107I	GGYRCC	5 cut(s)	117, 304, 1204, 2240, 2909
BspTNI	GGTCTC	1 cut(s)	376
BsrFI	RCCGGY	2 cut(s)	131, 2912
BsrI	ACTGG	6 cut(s)	76, 386, 1018, 1025, 1475, 2486
BssAI	RCCGGY	2 cut(s)	131, 2912
BssECI	CCNNGG	7 cut(s)	76, 276, 308, 556, 739, 777, 1984
BssNI	GRCGYC	1 cut(s)	35
BssT1I	CCWWGG	2 cut(s)	276, 1984
Bst6I	CTCTTC	7 cut(s)	9, 209, 720, 1289, 1376, 1469, 1939
BstACI	GRCGYC	1 cut(s)	35
BstAPI	GCANNNNNTGC	1 cut(s)	1832
BstBAI	YACGTR	1 cut(s)	2518
BstBI	TTCGAA	1 cut(s)	211
BstDEI	CTNAG	6 cut(s)	1148, 1428, 1909, 2133, 2361, 2930
BstDSI	CCRYGG	2 cut(s)	76, 1984
BstF5I	GGATG	8 cut(s)	546, 681, 749, 1101, 1603, 1808, 2341, 2539
BstFNI	CGCG	2 cut(s)	99, 115
BstH2I	RGCGCY	1 cut(s)	1438
BstHHI	GCGC	3 cut(s)	115, 1437, 2273
BstMAI	GTCTC	8 cut(s)	287, 376, 657, 1106, 1693, 1936, 2295, 2953
BstNSI	RCATGY	2 cut(s)	1634, 2091
BstSFI	CTRYAG	6 cut(s)	675, 1106, 1452, 2647, 2653, 2819
BstSNI	TACGTA	1 cut(s)	2518
BstUI	CGCG	2 cut(s)	99, 115
BstV2I	GAAGAC	2 cut(s)	166, 1329
BstX2I	RGATCY	6 cut(s)	626, 1345, 1735, 1804, 2008, 2225
BstXI	CCANNNNNNTGG	1 cut(s)	711
BstYI	RGATCY	6 cut(s)	626, 1345, 1735, 1804, 2008, 2225
Bsu15I	ATCGAT	1 cut(s)	2322
BsuI	GTATCC	1 cut(s)	554
BsuRI	GGCC	5 cut(s)	75, 87, 135, 1640, 2163
BsuTUI	ATCGAT	1 cut(s)	2322
BtgI	CCRYGG	2 cut(s)	76, 1984
BtgZI	GCGATG	2 cut(s)	182, 2129
BtrI	CACGTC	1 cut(s)	148
BtsCI	GGATG	8 cut(s)	546, 681, 749, 1101, 1603, 1808, 2341, 2539
BtsI	GCAGTG	2 cut(s)	344, 2579
BveI	ACCTGC	4 cut(s)	698, 1826, 2397, 2539
CaiI	CAGNNNCTG	2 cut(s)	407, 845
CfoI	GCGC	3 cut(s)	115, 1437, 2273
Cfr10I	RCCGGY	2 cut(s)	131, 2912
Cfr13I	GGNCC	3 cut(s)	1639, 2071, 2425
ClaI	ATCGAT	1 cut(s)	2322
CseI	GACGC	2 cut(s)	24, 88
CsiI	ACCWGGT	1 cut(s)	48
Csp6I	GTAC	8 cut(s)	599, 898, 920, 1205, 1871, 2696, 2705, 2857
CspCI	CAANNNNNGTGG	2 cut(s)	676, 711
CviQI	GTAC	8 cut(s)	599, 898, 920, 1205, 1871, 2696, 2705, 2857
DdeI	CTNAG	6 cut(s)	1148, 1428, 1909, 2133, 2361, 2930
DraIII	CACNNNGTG	1 cut(s)	2060
EaeI	YGGCCR	4 cut(s)	73, 85, 133, 2161
Eam1104I	CTCTTC	7 cut(s)	9, 209, 720, 1289, 1376, 1469, 1939
EarI	CTCTTC	7 cut(s)	9, 209, 720, 1289, 1376, 1469, 1939
EciI	GGCGGA	1 cut(s)	539
Ecl136II	GAGCTC	2 cut(s)	161, 1299
Eco105I	TACGTA	1 cut(s)	2518
Eco130I	CCWWGG	2 cut(s)	276, 1984
Eco24I	GRGCYC	3 cut(s)	163, 1301, 2900
Eco31I	GGTCTC	1 cut(s)	376
Eco47I	GGWCC	2 cut(s)	2071, 2425
Eco53kI	GAGCTC	2 cut(s)	161, 1299
Eco57I	CTGAAG	2 cut(s)	427, 1341
Eco88I	CYCGRG	1 cut(s)	309
EcoICRI	GAGCTC	2 cut(s)	161, 1299
EcoO109I	RGGNCCY	1 cut(s)	2425
EcoRI	GAATTC	1 cut(s)	417
EcoT14I	CCWWGG	2 cut(s)	276, 1984
EcoT22I	ATGCAT	1 cut(s)	1138
EcoT38I	GRGCYC	3 cut(s)	163, 1301, 2900
ErhI	CCWWGG	2 cut(s)	276, 1984
Esp3I	CGTCTC	2 cut(s)	287, 657
FalI	AAGNNNNNCTT	2 cut(s)	1778, 1810
FaqI	GGGAC	6 cut(s)	464, 1016, 1898, 1986, 2329, 2761
FauI	CCCGC	1 cut(s)	638
FauNDI	CATATG	4 cut(s)	1693, 1774, 1828, 2311
FbaI	TGATCA	2 cut(s)	2473, 2605
FokI	GGATG	8 cut(s)	533, 668, 756, 1088, 1590, 1795, 2348, 2526
FriOI	GRGCYC	3 cut(s)	163, 1301, 2900
FspBI	CTAG	4 cut(s)	605, 1397, 1865, 2535
FspI	TGCGCA	1 cut(s)	2272
GlaI	GCGC	3 cut(s)	114, 1436, 2272
GsuI	CTGGAG	2 cut(s)	147, 504
HaeII	RGCGCY	1 cut(s)	1438
HaeIII	GGCC	5 cut(s)	75, 87, 135, 1640, 2163
HapII	CCGG	7 cut(s)	132, 156, 1438, 1642, 2560, 2784, 2913
HgaI	GACGC	2 cut(s)	24, 88
HhaI	GCGC	3 cut(s)	115, 1437, 2273
Hin1I	GRCGYC	1 cut(s)	35
Hin6I	GCGC	3 cut(s)	113, 1435, 2271
HinP1I	GCGC	3 cut(s)	113, 1435, 2271
HindIII	AAGCTT	2 cut(s)	803, 2105
HinfI	GANTC	8 cut(s)	124, 187, 1333, 1400, 1461, 2038, 2186, 2845
HpaII	CCGG	7 cut(s)	132, 156, 1438, 1642, 2560, 2784, 2913
HphI	GGTGA	9 cut(s)	145, 337, 427, 652, 1097, 1263, 2105, 2459, 2520
Hpy166II	GTNNAC	5 cut(s)	145, 698, 1256, 1613, 1871
Hpy8I	GTNNAC	5 cut(s)	145, 698, 1256, 1613, 1871
Hpy99I	CGWCG	6 cut(s)	40, 104, 107, 152, 2744, 2905
HpyAV	CCTTC	9 cut(s)	17, 715, 1061, 1649, 1774, 1913, 2438, 2581, 2802
HpyCH4IV	ACGT	7 cut(s)	66, 147, 1515, 1724, 1873, 2517, 2750
HpyF3I	CTNAG	6 cut(s)	1148, 1428, 1909, 2133, 2361, 2930
HpySE526I	ACGT	7 cut(s)	66, 147, 1515, 1724, 1873, 2517, 2750
Hsp92I	GRCGYC	1 cut(s)	35
HspAI	GCGC	3 cut(s)	113, 1435, 2271
KpnI	GGTACC	1 cut(s)	1208
KroI	GCCGGC	2 cut(s)	131, 2912
KroNI	GCCGGC	2 cut(s)	133, 2914
Ksp22I	TGATCA	2 cut(s)	2473, 2605
LguI	GCTCTTC	2 cut(s)	1289, 1376
LmnI	GCTCC	7 cut(s)	158, 166, 233, 1304, 2051, 2678, 2759
MabI	ACCWGGT	1 cut(s)	48
MaeI	CTAG	4 cut(s)	605, 1397, 1865, 2535
MaeII	ACGT	7 cut(s)	66, 147, 1515, 1724, 1873, 2517, 2750
MaeIII	GTNAC	8 cut(s)	67, 151, 433, 670, 903, 1016, 2488, 2642
MflI	RGATCY	6 cut(s)	626, 1345, 1735, 1804, 2008, 2225
MhlI	GDGCHC	5 cut(s)	163, 1301, 1501, 2056, 2900
MlsI	TGGCCA	2 cut(s)	75, 2163
MluNI	TGGCCA	2 cut(s)	75, 2163
MlyI	GAGTC	1 cut(s)	1455
MmeI	TCCRAC	8 cut(s)	692, 1491, 2029, 2188, 2234, 2310, 2763, 2785
Mox20I	TGGCCA	2 cut(s)	75, 2163
Mph1103I	ATGCAT	1 cut(s)	1138
MroNI	GCCGGC	2 cut(s)	131, 2912
MroXI	GAANNNNTTC	2 cut(s)	2185, 2454
MscI	TGGCCA	2 cut(s)	75, 2163
MseI	TTAA	6 cut(s)	254, 1706, 1991, 2372, 2421, 2565
MslI	CAYNNNNRTG	2 cut(s)	2086, 2403
Msp20I	TGGCCA	2 cut(s)	75, 2163
MspA1I	CMGCKG	2 cut(s)	404, 1457
MspI	CCGG	7 cut(s)	132, 156, 1438, 1642, 2560, 2784, 2913
MvnI	CGCG	2 cut(s)	99, 115
NaeI	GCCGGC	2 cut(s)	133, 2914
NciI	CCSGG	3 cut(s)	1439, 1642, 2561
NcoI	CCATGG	1 cut(s)	1984
NdeI	CATATG	4 cut(s)	1693, 1774, 1828, 2311
NgoMIV	GCCGGC	2 cut(s)	131, 2912
NlaIV	GGNNCC	8 cut(s)	119, 306, 1206, 2242, 2674, 2761, 2782, 2911
NmuCI	GTSAC	5 cut(s)	67, 151, 433, 1016, 2642
NsbI	TGCGCA	1 cut(s)	2272
NsiI	ATGCAT	1 cut(s)	1138
NspI	RCATGY	2 cut(s)	1634, 2091
NspV	TTCGAA	1 cut(s)	211
PaqCI	CACCTGC	2 cut(s)	1826, 2539
PciI	ACATGT	1 cut(s)	1630
PciSI	GCTCTTC	2 cut(s)	1289, 1376
PcsI	WCGNNNNNNNCGW	5 cut(s)	18, 63, 96, 135, 144
PdiI	GCCGGC	2 cut(s)	133, 2914
PdmI	GAANNNNTTC	2 cut(s)	2185, 2454
PfeI	GAWTC	7 cut(s)	124, 187, 1333, 1400, 2038, 2186, 2845
PflMI	CCANNNNNTGG	1 cut(s)	2032
PleI	GAGTC	1 cut(s)	1455
PpsI	GAGTC	1 cut(s)	1455
Ppu21I	YACGTR	1 cut(s)	2518
PpuMI	RGGWCCY	1 cut(s)	2425
PscI	ACATGT	1 cut(s)	1630
Psp124BI	GAGCTC	2 cut(s)	163, 1301
Psp1406I	AACGTT	1 cut(s)	1724
Psp5II	RGGWCCY	1 cut(s)	2425
PspN4I	GGNNCC	8 cut(s)	119, 306, 1206, 2242, 2674, 2761, 2782, 2911
PspPI	GGNCC	3 cut(s)	1639, 2071, 2425
PspPPI	RGGWCCY	1 cut(s)	2425
PsrI	GAACNNNNNNTAC	2 cut(s)	1854, 1886
PstI	CTGCAG	3 cut(s)	1110, 1456, 2823
PstNI	CAGNNNCTG	2 cut(s)	407, 845
PsuI	RGATCY	6 cut(s)	626, 1345, 1735, 1804, 2008, 2225
PvuII	CAGCTG	1 cut(s)	1457
RsaI	GTAC	8 cut(s)	600, 899, 921, 1206, 1872, 2697, 2706, 2858
RsaNI	GTAC	8 cut(s)	599, 898, 920, 1205, 1871, 2696, 2705, 2857
RseI	CAYNNNNRTG	2 cut(s)	2086, 2403
SacI	GAGCTC	2 cut(s)	163, 1301
SapI	GCTCTTC	2 cut(s)	1289, 1376
SaqAI	TTAA	6 cut(s)	254, 1706, 1991, 2372, 2421, 2565
Sau96I	GGNCC	3 cut(s)	1639, 2071, 2425
SbfI	CCTGCAGG	1 cut(s)	1110
SchI	GAGTC	1 cut(s)	1455
SdaI	CCTGCAGG	1 cut(s)	1110
SduI	GDGCHC	5 cut(s)	163, 1301, 1501, 2056, 2900
SexAI	ACCWGGT	1 cut(s)	48
SfcI	CTRYAG	6 cut(s)	675, 1106, 1452, 2647, 2653, 2819
SfuI	TTCGAA	1 cut(s)	211
SinI	GGWCC	2 cut(s)	2071, 2425
SmiMI	CAYNNNNRTG	2 cut(s)	2086, 2403
SmlI	CTYRAG	4 cut(s)	952, 965, 1034, 2459
SmoI	CTYRAG	4 cut(s)	952, 965, 1034, 2459
SnaBI	TACGTA	1 cut(s)	2518
Sse8387I	CCTGCAGG	1 cut(s)	1110
SsiI	CCGC	8 cut(s)	115, 404, 550, 631, 1247, 2216, 2664, 2941
SspMI	CTAG	4 cut(s)	605, 1397, 1865, 2535
SstI	GAGCTC	2 cut(s)	163, 1301
StyI	CCWWGG	2 cut(s)	276, 1984
TaiI	ACGT	7 cut(s)	69, 150, 1518, 1727, 1876, 2520, 2753
TauI	GCSGC	3 cut(s)	118, 407, 2943
TfiI	GAWTC	7 cut(s)	124, 187, 1333, 1400, 2038, 2186, 2845
Tru1I	TTAA	6 cut(s)	254, 1706, 1991, 2372, 2421, 2565
Tru9I	TTAA	6 cut(s)	254, 1706, 1991, 2372, 2421, 2565
TseFI	GTSAC	5 cut(s)	67, 151, 433, 1016, 2642
Tsp45I	GTSAC	5 cut(s)	67, 151, 433, 1016, 2642
TspDTI	ATGAA	9 cut(s)	196, 787, 954, 1080, 1322, 1328, 1340, 2361, 2565
TspGWI	ACGGA	4 cut(s)	344, 572, 777, 1770
Van91I	CCANNNNNTGG	1 cut(s)	2032
VpaK11BI	GGWCC	2 cut(s)	2071, 2425
XapI	RAATTY	7 cut(s)	417, 793, 1482, 2154, 2554, 2596, 2835
XceI	RCATGY	2 cut(s)	1634, 2091
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1815
XmnI	GAANNNNTTC	2 cut(s)	2185, 2454
XspI	CTAG	4 cut(s)	605, 1397, 1865, 2535
Zsp2I	ATGCAT	1 cut(s)	1138

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.