Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0006249

Physical Location & Seq

Genomic Coordinates Forward (+)

5203605 .. 5212846

Gene Structure 9242 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00162820.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2478 bp

ATGAGGATTGAGGAAGAGGCCGACGGAGTCTCCGCGTCCAGGAACCACCTCGTCTTCGCTTACTACGTCACCGGCCACGGCTTCGGCCACGCCACTCGCGTCGTCGAGGTGGTGCGTCACCTGATTCTCGCCGGACATGACGTTCACGTGGTCACCGGAGCTCCGGACTTTGTTTTCACGTCGGAGGTGCAGTCGCCTCGACTCTTCATTCGCAAGGTGCTGCTGGACTGTGGAGCGGTTCAGGCGGATGCCTTAACAGTGGATCGCCTTGCATCTTTGGAGAAGTATTCGGAGACGGCTGTGGTGCCAAGGGAGTCTATATTGAAAACTGAAGTAGAGTGGCTGACCTCCATCAAGGCTGACCTGGTGGTCTCGGATGTTGTTCCAGTTGCGTGCCGAGCTGCAGCTGATGCTGGAATTCGCTCCGTCTGTGTCACTAACTTTAGCTGGGACTTCATCTATGCAGAATATGTCATGGCAGCCGGAAGTCATCATCGTTCAATTGTTTGGCAGATAGCAGAAGATTATTCTCACTGTGAGTTCCTGATCCGCCTCCCAGGATACTGCCCAATGCCTGCATTCCGTGATGTAATTGACGTACCTTTAGTTGTGAGGAGGTTGCACAGATCCCGCAAAGAGGTGAGAAAGGAACTAGGAATTGAAGAAGATGCCAAATTAGTCATTCTCAACTTTGGTGGACAGCCATCAGGCTGGAAGTTGAAAGAGGAGTTTTTACCCCTGGATGGCTTGGCCTGCCAGGAGCTTCCACCAAATTTTAGGAAGCTTGCAAAAGATGCTTATACACCTGATATTATAGCAGCATCTGATTGTATGCTGGCTCAGGTCCTTCAAGAAACAGCTATTGGGAAAAACTGGGCATCAGATAAGCTTAGTGGGGCAAGAAGGTTGCGTGATGCAATAATTCTTGGATGCATTGAAGACTTTGAGATTCTTCATGGAGATCTTCAAGGTCTTTCCGATACAATGACTTTTTTGAAGAGCTTGGCAGAACTAGATTCTGCATATGAATCTGAAAAAGCTACTGAGAAGCGGCAGATAAGAGAGCGGAAGGCTGCAGCTGGACTCTTCAATTGGGAGGAAGAAATTTTTGTGGCAAGAGCACCTGGACGATTAGATGTAATGGGTGGGATTGCTGACTATTCAGGAAGCCTTGTCTTGCAGATGCCCATAAGAGAAGCTTGCCATGTTGCTGTACAGAGACATCAACCTAGTAAACACAGGCTCTGGAAGCATGCCCTGGCTCGGCAGGAAGCCAAAGGACAAAGCTCAACACCTGTCTTGCAAATTGTCTCGTATGGTTCAGAATTGAGCAATCGTAGTCCTACTTTTGACATGGATCTATCTGATTTTATGGACGGAGATCATCCAATGTGGGCAGCATATGTTGCGGGTGTCATTCTGGTTTTAATGACAGAACTAGGTGTACGCTTTAAGGAGAGCATTAGTTTGCTGGTATCCTCTTTAGTTCCGGAAGGCAAAGGTGTCTCTTCTTCTGCTTCGGTGGAGGTTGCTACAATGTCTGCTATAGCTGCTGCCCATGAATTAAGCATCAGTCCCAGAGATCTTGCTTTGCTTTGCCAGAAGGTTGCACCTTTAGCTAACTTGACACATCCAAACCCTTTTCTTTGTCCATTTACTCCTGCTGAGGTACTTGGGCTTGTAGAAATTCCTGGCCACGTCCGATTTTGGGGAATCGATTCAGGCATAAGACACAGTGTTGGTGGTGCGGATTATGGATCTGTTAGAGTAGGTGCCTTTATGGGTCGGACGATCATAAAGTCTACAGCTTCTACGATTATGTCTCAATCATTGCCAAATGGAAATGGGATGAATCTTGACGAGTTAGAGGATGATGGTTTTGAACTGCCTAAAGCTGAAGCTTCATTAGATTATTTATACGATGCTCTTTATGTTAAGATGCTTCCCGAGTCCATACTTGGTGAGGCTTTTCTGGAGAAGTACGCTGATCATGGTGACCCAGTTACAGTTATTGATCCAAAGTGTAACTATGGAGTGAGAGCACCTACTAGACATCCTATATATGAAAATTTCCGAGTTAAGGCATTCAAAGCACTTCTAACTTCTGCAAATTCAGATGATCAGCTTACGGCTCTTGGAGAGTTATTGTATCAGTGCCACTACAGCTACAGTGCCTGTGGACTTGGCTCGGATGGAACAAATAGGCTTGTACAGTTGGTGCAAGAGATGCAGCATAGTAAATCATCTAAAATAGATGGTGGGGCATTATATGGAGCAAAGATTACGGGTGGAGGTTCTGGCGGAACAGTTTGCGCTGTTGGCAGGAATTGCCTGAAGAGCAGCCAACAAATATTTGAGATTCAGCAGAGATACAAAGCCGCAACTGGGTATTTGCCATATATTTTTGAGGGCTCATCACCAGGTGCAGGCAAATTTGGCCATCTAAGAATCCGGCGCCGCTCTGTCAAGCTGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0005975 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009506 GO:0009702 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0030054 GO:0044237 GO:0044238 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046835 GO:0055044 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

GHMP_kinases_C GHMP_kinases_N GalKase_gal_bdg Glyco_trans_1_3

Protein Analysis

825

Amino Acids

90.41

Weight (kDa)

6.0

Isoelectric Point (pI)

42.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GalKase_gal_bdg	PF10509	366 - 407	3.1e-07	Galactokinase galactose-binding signature
GHMP_kinases_N	PF00288	468 - 537	2.5e-09	GHMP kinases N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G42850 AT3G42850 AT4G16130
fragaria_vesca	FvH4_2g36460 FvH4_2g36460 FvH4_2g36460
malus_domestica	MD08G1124400.v1.1 MD15G1103100.v1.1
prunus_persica	Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1
pyrus_communis	pycom08g10310 pycom15g09400
rosa_chinensis	RchiOBHm_Chr6g0305421
rosa_laevigata	RLG00000010892
rosa_multiflora	Rmu_sc0002782.1_g000020
rosa_roxburghii	Rroxscaffold_7G00162820
rosa_rugosa	Rorug06G0340200
rosa_samantha	Rh6AG452200 Rh6BG449500 Rh6CG466000 Rh6DG452700
rosa_wichuraiana	Rw6G039400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	368
AccB1I	GGYRCC	3 cut(s)	304, 1772, 2454
AccBSI	CCGCTC	3 cut(s)	236, 1066, 2460
AccI	GTMKAC	1 cut(s)	1802
AccII	CGCG	2 cut(s)	35, 99
AccIII	TCCGGA	2 cut(s)	163, 1489
AclWI	GGATC	6 cut(s)	270, 541, 621, 1365, 1765, 2009
AcoI	YGGCCR	4 cut(s)	73, 85, 1693, 2437
AcsI	RAATTY	7 cut(s)	417, 772, 1104, 1686, 2068, 2110, 2432
AcuI	CTGAAG	3 cut(s)	351, 1917, 2354
AcvI	CACGTG	1 cut(s)	148
AcyI	GRCGYC	1 cut(s)	2455
AdeI	CACNNNGTG	1 cut(s)	2423
AfaI	GTAC	6 cut(s)	600, 1215, 1446, 1671, 1982, 2211
AfiI	CCNNNNNNNGG	8 cut(s)	39, 637, 743, 1263, 1708, 2080, 2385, 2426
AjiI	CACGTC	2 cut(s)	180, 1699
AjnI	CCWGG	9 cut(s)	38, 363, 556, 738, 756, 1123, 1257, 1690, 2419
AjuI	GAANNNNNNNTTGG	2 cut(s)	846, 878
AloI	GAACNNNNNNTCC	2 cut(s)	126, 158
Alw21I	GWGCWC	3 cut(s)	163, 1123, 2044
Alw26I	GTCTC	7 cut(s)	34, 287, 376, 1213, 1315, 1510, 1827
AlwI	GGATC	6 cut(s)	270, 541, 621, 1365, 1765, 2009
AlwNI	CAGNNNCTG	3 cut(s)	824, 1245, 2175
Ama87I	CYCGRG	1 cut(s)	1946
Aor13HI	TCCGGA	2 cut(s)	163, 1489
AoxI	GGCC	6 cut(s)	18, 73, 85, 750, 1693, 2437
ApoI	RAATTY	7 cut(s)	417, 772, 1104, 1686, 2068, 2110, 2432
ArsI	GACNNNNNNTTYG	2 cut(s)	192, 224
Asp700I	GAANNNNTTC	1 cut(s)	1717
AspLEI	GCGC	2 cut(s)	2315, 2457
AspS9I	GGNCC	1 cut(s)	844
AsuHPI	GGTGA	7 cut(s)	61, 110, 145, 652, 1973, 2006, 2409
AvaI	CYCGRG	1 cut(s)	1946
AvaII	GGWCC	1 cut(s)	844
BalI	TGGCCA	2 cut(s)	1695, 2439
BanI	GGYRCC	3 cut(s)	304, 1772, 2454
BanII	GRGCYC	2 cut(s)	163, 2414
BbrPI	CACGTG	1 cut(s)	148
BbsI	GAAGAC	2 cut(s)	46, 945
Bbv12I	GWGCWC	3 cut(s)	163, 1123, 2044
BbvCI	CCTCAGC	1 cut(s)	1665
BccI	CCATC	7 cut(s)	359, 712, 737, 1868, 2186, 2249, 2448
BceAI	ACGGC	3 cut(s)	94, 312, 2145
BcgI	CGANNNNNNTGC	2 cut(s)	179, 213
BciT130I	CCWGG	9 cut(s)	40, 365, 558, 740, 758, 1125, 1259, 1692, 2421
BciVI	GTATCC	2 cut(s)	554, 1486
BclI	TGATCA	2 cut(s)	1987, 2119
BcoDI	GTCTC	7 cut(s)	34, 287, 376, 1213, 1315, 1510, 1827
BfaI	CTAG	6 cut(s)	653, 1013, 1230, 1439, 2049, 2476
BfmI	CTRYAG	6 cut(s)	402, 1074, 1545, 1803, 2161, 2167
BfoI	RGCGCY	1 cut(s)	2458
BfuI	GTATCC	2 cut(s)	554, 1486
BglII	AGATCT	2 cut(s)	961, 1582
Bme1390I	CCNGG	9 cut(s)	40, 365, 558, 740, 758, 1125, 1259, 1692, 2421
Bme18I	GGWCC	1 cut(s)	844
BmeT110I	CYCGRG	1 cut(s)	1946
BmgBI	CACGTC	2 cut(s)	180, 1699
BmgT120I	GGNCC	1 cut(s)	844
BmiI	GGNNCC	4 cut(s)	44, 306, 1774, 2456
BmrFI	CCNGG	9 cut(s)	40, 365, 558, 740, 758, 1125, 1259, 1692, 2421
BmrI	ACTGGG	3 cut(s)	883, 1994, 2394
BmuI	ACTGGG	3 cut(s)	883, 1994, 2394
BpiI	GAAGAC	2 cut(s)	46, 945
BpmI	CTGGAG	1 cut(s)	1994
Bpu10I	CCTNAGC	2 cut(s)	840, 1665
Bsa29I	ATCGAT	1 cut(s)	1716
BsaAI	YACGTR	1 cut(s)	148
BsaHI	GRCGYC	1 cut(s)	2455
BsaI	GGTCTC	1 cut(s)	376
BsaJI	CCNNGG	5 cut(s)	76, 308, 556, 738, 1257
BsaWI	WCCGGW	3 cut(s)	155, 163, 1489
BsaXI	ACNNNNNCTCC	6 cut(s)	14, 44, 145, 175, 176, 206
Bsc4I	CCNNNNNNNGG	8 cut(s)	39, 637, 743, 1263, 1708, 2080, 2385, 2426
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	4 cut(s)	386, 878, 2000, 2389
Bse3DI	GCAATG	1 cut(s)	1829
BseAI	TCCGGA	2 cut(s)	163, 1489
BseBI	CCWGG	9 cut(s)	40, 365, 558, 740, 758, 1125, 1259, 1692, 2421
BseCI	ATCGAT	1 cut(s)	1716
BseDI	CCNNGG	5 cut(s)	76, 308, 556, 738, 1257
BseLI	CCNNNNNNNGG	8 cut(s)	39, 637, 743, 1263, 1708, 2080, 2385, 2426
BseMI	GCAATG	1 cut(s)	1829
BseMII	CTCAG	3 cut(s)	854, 1035, 1656
BseNI	ACTGG	4 cut(s)	386, 878, 2000, 2389
BseRI	GAGGAG	2 cut(s)	628, 740
BseYI	CCCAGC	1 cut(s)	447
BsgI	GTGCAG	2 cut(s)	209, 2445
Bsh1236I	CGCG	2 cut(s)	35, 99
BshFI	GGCC	6 cut(s)	20, 75, 87, 752, 1695, 2439
BshNI	GGYRCC	3 cut(s)	304, 1772, 2454
BshVI	ATCGAT	1 cut(s)	1716
BsiHKAI	GWGCWC	3 cut(s)	163, 1123, 2044
BsiHKCI	CYCGRG	1 cut(s)	1946
BsiSI	CCGG	7 cut(s)	72, 132, 156, 164, 483, 1490, 2452
BslFI	GGGAC	2 cut(s)	464, 1560
BslI	CCNNNNNNNGG	8 cut(s)	39, 637, 743, 1263, 1708, 2080, 2385, 2426
BsmAI	GTCTC	7 cut(s)	34, 287, 376, 1213, 1315, 1510, 1827
BsmBI	CGTCTC	1 cut(s)	287
BsmFI	GGGAC	2 cut(s)	464, 1560
BsmI	GAATGC	2 cut(s)	578, 2084
BsnI	GGCC	6 cut(s)	20, 75, 87, 752, 1695, 2439
Bso31I	GGTCTC	1 cut(s)	376
BsoBI	CYCGRG	1 cut(s)	1946
Bsp1286I	GDGCHC	4 cut(s)	163, 1123, 2044, 2414
Bsp13I	TCCGGA	2 cut(s)	163, 1489
Bsp1407I	TGTACA	2 cut(s)	1213, 2209
BspANI	GGCC	6 cut(s)	20, 75, 87, 752, 1695, 2439
BspCNI	CTCAG	3 cut(s)	853, 1036, 1657
BspDI	ATCGAT	1 cut(s)	1716
BspEI	TCCGGA	2 cut(s)	163, 1489
BspFNI	CGCG	2 cut(s)	35, 99
BspLI	GGNNCC	4 cut(s)	44, 306, 1774, 2456
BspMAI	CTGCAG	2 cut(s)	406, 1078
BspPI	GGATC	6 cut(s)	270, 541, 621, 1365, 1765, 2009
BspQI	GCTCTTC	2 cut(s)	992, 2330
BspT107I	GGYRCC	3 cut(s)	304, 1772, 2454
BspTNI	GGTCTC	1 cut(s)	376
BsrBI	CCGCTC	3 cut(s)	236, 1066, 2460
BsrDI	GCAATG	1 cut(s)	1829
BsrFI	RCCGGY	1 cut(s)	71
BsrGI	TGTACA	2 cut(s)	1213, 2209
BsrI	ACTGG	4 cut(s)	386, 878, 2000, 2389
BssAI	RCCGGY	1 cut(s)	71
BssECI	CCNNGG	5 cut(s)	76, 308, 556, 738, 1257
BssNI	GRCGYC	1 cut(s)	2455
BssT1I	CCWWGG	1 cut(s)	308
Bst2UI	CCWGG	9 cut(s)	40, 365, 558, 740, 758, 1125, 1259, 1692, 2421
Bst4CI	ACNGT	8 cut(s)	230, 259, 536, 1736, 2008, 2171, 2214, 2308
Bst6I	CTCTTC	6 cut(s)	9, 209, 992, 1091, 1513, 2330
BstACI	GRCGYC	1 cut(s)	2455
BstAPI	GCANNNNNTGC	5 cut(s)	410, 794, 1406, 2227, 2328
BstAUI	TGTACA	2 cut(s)	1213, 2209
BstBAI	YACGTR	1 cut(s)	148
BstC8I	GCNNGC	8 cut(s)	394, 576, 754, 786, 837, 1201, 1254, 2428
BstDEI	CTNAG	5 cut(s)	840, 890, 1044, 1665, 2444
BstDSI	CCRYGG	1 cut(s)	76
BstEII	GGTNACC	2 cut(s)	151, 1994
BstFNI	CGCG	2 cut(s)	35, 99
BstH2I	RGCGCY	1 cut(s)	2458
BstHHI	GCGC	2 cut(s)	2315, 2457
BstMAI	GTCTC	7 cut(s)	34, 287, 376, 1213, 1315, 1510, 1827
BstNI	CCWGG	9 cut(s)	40, 365, 558, 740, 758, 1125, 1259, 1692, 2421
BstNSI	RCATGY	1 cut(s)	1256
BstPI	GGTNACC	2 cut(s)	151, 1994
BstSCI	CCNGG	9 cut(s)	38, 363, 556, 738, 756, 1123, 1257, 1690, 2419
BstSFI	CTRYAG	6 cut(s)	402, 1074, 1545, 1803, 2161, 2167
BstUI	CGCG	2 cut(s)	35, 99
BstV2I	GAAGAC	2 cut(s)	46, 945
BstX2I	RGATCY	5 cut(s)	626, 961, 1357, 1582, 1757
BstXI	CCANNNNNNTGG	1 cut(s)	711
BstYI	RGATCY	5 cut(s)	626, 961, 1357, 1582, 1757
Bsu15I	ATCGAT	1 cut(s)	1716
BsuI	GTATCC	2 cut(s)	554, 1486
BsuRI	GGCC	6 cut(s)	20, 75, 87, 752, 1695, 2439
BsuTUI	ATCGAT	1 cut(s)	1716
BtgI	CCRYGG	1 cut(s)	76
BtrI	CACGTC	2 cut(s)	180, 1699
BtsIMutI	CAGTG	5 cut(s)	264, 532, 1741, 2159, 2176
Cac8I	GCNNGC	8 cut(s)	394, 576, 754, 786, 837, 1201, 1254, 2428
CaiI	CAGNNNCTG	3 cut(s)	824, 1245, 2175
CfoI	GCGC	2 cut(s)	2315, 2457
Cfr10I	RCCGGY	1 cut(s)	71
Cfr13I	GGNCC	1 cut(s)	844
ClaI	ATCGAT	1 cut(s)	1716
CseI	GACGC	3 cut(s)	24, 88, 104
CsiI	ACCWGGT	2 cut(s)	363, 2419
Csp6I	GTAC	6 cut(s)	599, 1214, 1445, 1670, 1981, 2210
CspCI	CAANNNNNGTGG	2 cut(s)	676, 711
CviAII	CATG	8 cut(s)	137, 475, 956, 1205, 1253, 1354, 1559, 1991
CviQI	GTAC	6 cut(s)	599, 1214, 1445, 1670, 1981, 2210
DdeI	CTNAG	5 cut(s)	840, 890, 1044, 1665, 2444
DinI	GGCGCC	1 cut(s)	2456
DraIII	CACNNNGTG	1 cut(s)	2423
DrdI	GACNNNNNNGTC	1 cut(s)	368
DseDI	GACNNNNNNGTC	1 cut(s)	368
EaeI	YGGCCR	4 cut(s)	73, 85, 1693, 2437
Eam1104I	CTCTTC	6 cut(s)	9, 209, 992, 1091, 1513, 2330
EarI	CTCTTC	6 cut(s)	9, 209, 992, 1091, 1513, 2330
EciI	GGCGGA	3 cut(s)	260, 539, 2316
Ecl136II	GAGCTC	1 cut(s)	161
Eco130I	CCWWGG	1 cut(s)	308
Eco24I	GRGCYC	2 cut(s)	163, 2414
Eco31I	GGTCTC	1 cut(s)	376
Eco47I	GGWCC	1 cut(s)	844
Eco53kI	GAGCTC	1 cut(s)	161
Eco57I	CTGAAG	3 cut(s)	351, 1917, 2354
Eco72I	CACGTG	1 cut(s)	148
Eco88I	CYCGRG	1 cut(s)	1946
Eco91I	GGTNACC	2 cut(s)	151, 1994
EcoICRI	GAGCTC	1 cut(s)	161
EcoO109I	RGGNCCY	1 cut(s)	844
EcoO65I	GGTNACC	2 cut(s)	151, 1994
EcoRI	GAATTC	1 cut(s)	417
EcoRII	CCWGG	9 cut(s)	38, 363, 556, 738, 756, 1123, 1257, 1690, 2419
EcoT14I	CCWWGG	1 cut(s)	308
EcoT22I	ATGCAT	1 cut(s)	935
EcoT38I	GRGCYC	2 cut(s)	163, 2414
EgeI	GGCGCC	1 cut(s)	2456
EheI	GGCGCC	1 cut(s)	2456
ErhI	CCWWGG	1 cut(s)	308
Esp3I	CGTCTC	1 cut(s)	287
FaeI	CATG	8 cut(s)	140, 478, 959, 1208, 1256, 1357, 1562, 1994
FalI	AAGNNNNNCTT	2 cut(s)	1183, 1215
FaqI	GGGAC	2 cut(s)	464, 1560
FatI	CATG	8 cut(s)	136, 474, 955, 1204, 1252, 1353, 1558, 1990
FauI	CCCGC	2 cut(s)	638, 1402
FauNDI	CATATG	2 cut(s)	1024, 1402
FbaI	TGATCA	2 cut(s)	1987, 2119
FblI	GTMKAC	1 cut(s)	1802
FriOI	GRGCYC	2 cut(s)	163, 2414
FspBI	CTAG	6 cut(s)	653, 1013, 1230, 1439, 2049, 2476
GlaI	GCGC	2 cut(s)	2314, 2456
GsaI	CCCAGC	1 cut(s)	451
GsuI	CTGGAG	1 cut(s)	1994
HaeII	RGCGCY	1 cut(s)	2458
HaeIII	GGCC	6 cut(s)	20, 75, 87, 752, 1695, 2439
HapII	CCGG	7 cut(s)	72, 132, 156, 164, 483, 1490, 2452
HgaI	GACGC	3 cut(s)	24, 88, 104
HhaI	GCGC	2 cut(s)	2315, 2457
Hin1I	GRCGYC	1 cut(s)	2455
Hin1II	CATG	8 cut(s)	140, 478, 959, 1208, 1256, 1357, 1562, 1994
Hin6I	GCGC	2 cut(s)	2313, 2455
HinP1I	GCGC	2 cut(s)	2313, 2455
HindIII	AAGCTT	4 cut(s)	782, 887, 1197, 1899
HpaII	CCGG	7 cut(s)	72, 132, 156, 164, 483, 1490, 2452
HphI	GGTGA	7 cut(s)	61, 110, 145, 652, 1973, 2006, 2409
Hpy166II	GTNNAC	6 cut(s)	145, 698, 1235, 1445, 1803, 2180
Hpy188III	TCNNGA	9 cut(s)	164, 544, 851, 1164, 1246, 1490, 1856, 1946, 1973
Hpy8I	GTNNAC	6 cut(s)	145, 698, 1235, 1445, 1803, 2180
Hpy99I	CGWCG	4 cut(s)	26, 104, 107, 184
HpyAV	CCTTC	5 cut(s)	857, 897, 1063, 1487, 1597
HpyCH4III	ACNGT	8 cut(s)	230, 259, 536, 1736, 2008, 2171, 2214, 2308
HpyCH4IV	ACGT	6 cut(s)	66, 141, 147, 179, 597, 1698
HpyF3I	CTNAG	5 cut(s)	840, 890, 1044, 1665, 2444
HpySE526I	ACGT	6 cut(s)	66, 141, 147, 179, 597, 1698
Hsp92I	GRCGYC	1 cut(s)	2455
Hsp92II	CATG	8 cut(s)	140, 478, 959, 1208, 1256, 1357, 1562, 1994
HspAI	GCGC	2 cut(s)	2313, 2455
KasI	GGCGCC	1 cut(s)	2454
Kpn2I	TCCGGA	2 cut(s)	163, 1489
Ksp22I	TGATCA	2 cut(s)	1987, 2119
LguI	GCTCTTC	2 cut(s)	992, 2330
LmnI	GCTCC	6 cut(s)	158, 166, 233, 428, 760, 2273
MabI	ACCWGGT	2 cut(s)	363, 2419
MaeI	CTAG	6 cut(s)	653, 1013, 1230, 1439, 2049, 2476
MaeII	ACGT	6 cut(s)	66, 141, 147, 179, 597, 1698
MaeIII	GTNAC	7 cut(s)	67, 116, 151, 433, 1994, 2002, 2024
MbiI	CCGCTC	3 cut(s)	236, 1066, 2460
MfeI	CAATTG	2 cut(s)	501, 1090
MflI	RGATCY	5 cut(s)	626, 961, 1357, 1582, 1757
MhlI	GDGCHC	4 cut(s)	163, 1123, 2044, 2414
MlsI	TGGCCA	2 cut(s)	1695, 2439
MluNI	TGGCCA	2 cut(s)	1695, 2439
Mly113I	GGCGCC	1 cut(s)	2455
MlyI	GAGTC	5 cut(s)	36, 195, 323, 1077, 1958
MmeI	TCCRAC	2 cut(s)	162, 1766
Mox20I	TGGCCA	2 cut(s)	1695, 2439
Mph1103I	ATGCAT	1 cut(s)	935
MroI	TCCGGA	2 cut(s)	163, 1489
MroXI	GAANNNNTTC	1 cut(s)	1717
MscI	TGGCCA	2 cut(s)	1695, 2439
MseI	TTAA	6 cut(s)	254, 1427, 1452, 1565, 1935, 2079
Msp20I	TGGCCA	2 cut(s)	1695, 2439
MspA1I	CMGCKG	2 cut(s)	407, 1079
MspI	CCGG	7 cut(s)	72, 132, 156, 164, 483, 1490, 2452
MspR9I	CCNGG	9 cut(s)	40, 365, 558, 740, 758, 1125, 1259, 1692, 2421
MunI	CAATTG	2 cut(s)	501, 1090
Mva1269I	GAATGC	2 cut(s)	578, 2084
MvaI	CCWGG	9 cut(s)	40, 365, 558, 740, 758, 1125, 1259, 1692, 2421
MvnI	CGCG	2 cut(s)	35, 99
NarI	GGCGCC	1 cut(s)	2455
NdeI	CATATG	2 cut(s)	1024, 1402
NlaIII	CATG	8 cut(s)	140, 478, 959, 1208, 1256, 1357, 1562, 1994
NlaIV	GGNNCC	4 cut(s)	44, 306, 1774, 2456
NmeAIII	GCCGAG	2 cut(s)	422, 1243
NmuCI	GTSAC	5 cut(s)	67, 116, 151, 433, 1994
NsiI	ATGCAT	1 cut(s)	935
NspI	RCATGY	1 cut(s)	1256
PaeI	GCATGC	1 cut(s)	1256
PciSI	GCTCTTC	2 cut(s)	992, 2330
PcsI	WCGNNNNNNNCGW	2 cut(s)	63, 96
PctI	GAATGC	2 cut(s)	578, 2084
PdmI	GAANNNNTTC	1 cut(s)	1717
PfeI	GAWTC	9 cut(s)	124, 949, 1016, 1028, 1713, 1718, 1852, 2359, 2448
PflFI	GACNNNGTC	1 cut(s)	26
PfoI	TCCNGGA	1 cut(s)	38
PleI	GAGTC	5 cut(s)	35, 195, 322, 1077, 1957
PluTI	GGCGCC	1 cut(s)	2458
PmaCI	CACGTG	1 cut(s)	148
PmlI	CACGTG	1 cut(s)	148
PpsI	GAGTC	5 cut(s)	35, 195, 322, 1077, 1957
Ppu21I	YACGTR	1 cut(s)	148
PpuMI	RGGWCCY	1 cut(s)	844
Psp124BI	GAGCTC	1 cut(s)	163
Psp5II	RGGWCCY	1 cut(s)	844
Psp6I	CCWGG	9 cut(s)	38, 363, 556, 738, 756, 1123, 1257, 1690, 2419
PspCI	CACGTG	1 cut(s)	148
PspEI	GGTNACC	2 cut(s)	151, 1994
PspFI	CCCAGC	1 cut(s)	447
PspGI	CCWGG	9 cut(s)	38, 363, 556, 738, 756, 1123, 1257, 1690, 2419
PspN4I	GGNNCC	4 cut(s)	44, 306, 1774, 2456
PspPI	GGNCC	1 cut(s)	844
PspPPI	RGGWCCY	1 cut(s)	844
PsrI	GAACNNNNNNTAC	2 cut(s)	1428, 1460
PstI	CTGCAG	2 cut(s)	406, 1078
PstNI	CAGNNNCTG	3 cut(s)	824, 1245, 2175
PsuI	RGATCY	5 cut(s)	626, 961, 1357, 1582, 1757
PsyI	GACNNNGTC	1 cut(s)	26
PvuII	CAGCTG	2 cut(s)	407, 1079
RsaI	GTAC	6 cut(s)	600, 1215, 1446, 1671, 1982, 2211
RsaNI	GTAC	6 cut(s)	599, 1214, 1445, 1670, 1981, 2210
SacI	GAGCTC	1 cut(s)	163
SapI	GCTCTTC	2 cut(s)	992, 2330
SaqAI	TTAA	6 cut(s)	254, 1427, 1452, 1565, 1935, 2079
Sau96I	GGNCC	1 cut(s)	844
SchI	GAGTC	5 cut(s)	36, 195, 323, 1077, 1958
ScrFI	CCNGG	9 cut(s)	40, 365, 558, 740, 758, 1125, 1259, 1692, 2421
SduI	GDGCHC	4 cut(s)	163, 1123, 2044, 2414
SexAI	ACCWGGT	2 cut(s)	363, 2419
SfcI	CTRYAG	6 cut(s)	402, 1074, 1545, 1803, 2161, 2167
SfoI	GGCGCC	1 cut(s)	2456
SinI	GGWCC	1 cut(s)	844
SphI	GCATGC	1 cut(s)	1256
SspDI	GGCGCC	1 cut(s)	2454
SspI	AATATT	1 cut(s)	2352
SspMI	CTAG	6 cut(s)	653, 1013, 1230, 1439, 2049, 2476
SstI	GAGCTC	1 cut(s)	163
StyD4I	CCNGG	9 cut(s)	38, 363, 556, 738, 756, 1123, 1257, 1690, 2419
StyI	CCWWGG	1 cut(s)	308
TaaI	ACNGT	8 cut(s)	230, 259, 536, 1736, 2008, 2171, 2214, 2308
TaiI	ACGT	6 cut(s)	69, 144, 150, 182, 600, 1701
TaqI	TCGA	3 cut(s)	105, 199, 1716
TatI	WGTACW	2 cut(s)	1213, 2209
TauI	GCSGC	3 cut(s)	1054, 2381, 2460
TfiI	GAWTC	9 cut(s)	124, 949, 1016, 1028, 1713, 1718, 1852, 2359, 2448
Tru1I	TTAA	6 cut(s)	254, 1427, 1452, 1565, 1935, 2079
Tru9I	TTAA	6 cut(s)	254, 1427, 1452, 1565, 1935, 2079
TscAI	CASTG	5 cut(s)	264, 539, 1741, 2159, 2176
TseFI	GTSAC	5 cut(s)	67, 116, 151, 433, 1994
Tsp45I	GTSAC	5 cut(s)	67, 116, 151, 433, 1994
TspDTI	ATGAA	8 cut(s)	196, 445, 944, 1041, 1575, 1865, 1893, 2079
TspGWI	ACGGA	4 cut(s)	39, 415, 572, 1392
TspRI	CASTG	5 cut(s)	264, 539, 1741, 2159, 2176
Tth111I	GACNNNGTC	1 cut(s)	26
VpaK11BI	GGWCC	1 cut(s)	844
XapI	RAATTY	7 cut(s)	417, 772, 1104, 1686, 2068, 2110, 2432
XceI	RCATGY	1 cut(s)	1256
XmiI	GTMKAC	1 cut(s)	1802
XmnI	GAANNNNTTC	1 cut(s)	1717
XspI	CTAG	6 cut(s)	653, 1013, 1230, 1439, 2049, 2476
Zsp2I	ATGCAT	1 cut(s)	935

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.