Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006249

Physical Location & Seq

Genomic Coordinates Forward (+)

11669411 .. 11677488

Gene Structure 8078 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1124400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2973 bp

ATGAGGATTGACGAAGAGGGCGAAGGAGTGTCGGCGTCCAGGAACCACCTGGTTTTTGCCTACTACGTCACCGGTCACGGATTCGGCCACGCCACCCGAGTGGTCGAGGTTGCGCGGCACCTGATTCTCGCCGGCCACGATGTCCACGTCGTCACCGGAGCTCCTGACTTCGTCTTCACTTCCGAGATACAATCGCCTCGCCTCTTCATCCGCAAGGTTCTTCTGGACTGTGGAGCTGTGCAAGCAGATGCTTTAACAGTCGATCGTCTTGCTTCCTTAGCTAAGTATTCCGAGACAGCTGTGGCGCCTCGGGCTTCAATCTTGAAAACGGAAGTGGAGTGGCTAACCTCCATCAAAGCTGACCTAGTGGTCTCAGATGTTGTTCCTGTTGCATGCCGTGCAGCCGCTGATGCTGGAATTCGCTCTGTTTGTGTCACTAACTTTAGTTGGGACTTCATCTATGCAGAATATGTCATGGCTGCTGGAAGTAATCATCGTTCAATCGTCTGGCAGATAGCAGAAGATTATTCTCACTGCGAGTTCCTCATCCGCCTCCCAGGATACTGCCCAATGCCTGCTTTCCGTGATGCAGTTGATGTACCTCTAGTTGTGAGGAGGTTTCGCAGATCCCGCAAAGAGGTGAGGGAGGAGCTTGGAATTGGAGATGATGTAAAACTAGTCATCCTCAACTTTGGTGGACAGCCAGCAGGCTGGAAGTTGAAGGAAGAGTTTTTGCCTCCTGGATGGCTGTGCCTGGTTTGTGGTGGTTCTGACACCCAGGAACTTCCATCAAATTTCATAAAACTGGCAAAAGATGCTTATACACCTGATTTTATGGCAGCATCTGACTGTATGCTTGGAAAAATTGGATACGGCACTGTGAGTGAAGCTCTTTCATACAAGTTACCATTTGTCTTTGTACGCAGAGATTATTTCAATGAAGAACCTTTCTTGAGAAATATGCTTGAGTATTATCAAGGTGGCGTTGAGATGATTAGGAGGGACTTGCTCACTGGTCATTGGAAACCTTACCTTGAGCGTGCAATCAGTTTGAAACCATGCTATGAAGGCGGCATCAATGGTGGTGAGGTGGCAGCTCACATCCTGCAAGAGACGGCTATTGGGAAAAACTACTCTTCAGATAAGCTAAGTGGGGCAAGAAGATTGCGTGATGCCATAATTCTTGGGTATCAACTTCAAAGGGCGCCTGGACGAGATATGGCAATTCCTGAATGGTATGCAAATGCTGAAAGTGAACTTGGAATTGGATCACCTACTTGCGGAATGAGCGAAAATGGCTCCCTAATTTACTCATGCACTGAAGATTTTGAGATTCTTCATGGAGATCTTCATGGTCTTTCAGATACAACGACTTTTTTGAAGAGCTTGGCGGAACTAGATTCTGTTTATGACTCTGAAAAAACTACTGAGAAGCGCCGGGAGCGCAAGGCTGCAGCTGGACTCTTCAACTGGGAGGAAGAAATTTTTGTAGCTAGAGCACCTGGACGGTTAGACGTAATGGGAGGGATTGCTGACTATTCAGGAAGCCTAGTCTTGCAGATGCCCATAAAAGAAGCCTGCCATGTTGCTGTTCAACGGAATCATCCAAGTAAACATAGGCTCTGGAAACATGCCCTGGCCCGGCAGCAGGCCCAAGGACAAAACCCAACGCCTGTCCTGCAAATTGTCTCATATGGTTCAGAGTTAAGCAACCGTAGCCCAACGTTTGACATGGATCTATCTGATTTTATGGATGGAGATAAGCCAATGTCATATGAGAATGCAAAGAAATACTTTTCACAAGATCCATCCCAAAAGTGGGCAGCATTTGTTGCAGGTGTCATTCTGGTTTTAATGACAGAACTAGGTGTACGTTTTGAGGATAGCATCAGTTTGCTGGTTTCCTCCGCCGTCCCAGAAGGCAAAGGTGTCTCTTCTTCTGCATCTGTGGAAGTCGCTACCATGTCTGCCATAGCTGCTGCCCATGGATTAAGCATCAGTCCCAGAGATCTTGCTTTGCTTTGCCAAAAGGTGGAGAATCATATTGTTGGAGCCCCATGCGGTGTGATGGACCAGATGACATCTGCATGTGGCGAAGCAAACAAGCTTCTTGCAATGGTGTGCCAGCCTGCTGAGGTTCTTGGACTTGTAGAAATTCCTGGCCATGTCAGATTTTGGGGAATTGATTCAGGAATAAGACACAGTGTCGGAGGTGCAGATTATGGATCTGTTCGAATAGGTGCCTTTATGGGTCGGAAGATGATAAAGTGTGCAGCTTCTACGATTTTGTGCCGGTCATCATCAACTGCAATTGGGACAAACGGTGATGAGTTGGAGGATGATGGTTTTGAACTACTTGAAGCTGAAGCCTCCTTAAATTACTTGTGCAACCTGTCACCTCACAGATATGAGGCTCTTTATGTTAAGGTGCTTCCTGAGACTATACTTGGTGAAACTTTTCTTGAGAAGTATGATGATCACAACGATCCAGTTACAGTAATTGATCCAAAATGTAACTACGTAGTAAGATCACCTGCTAGACATCCTATATATGAAAATTTCCGGGTTAAGACATTCAAGGCACTGCTAACATCTGCAAATTCAGATGATCAGCTTACAGCTCTTGGAGAATTACTGTATCAGTGCCACTACAGCTATAGTGCTTGCGGACTTGGCTCCGACGGAACAAACCGGCTTGTACGGTTGGTGCAAGCGATGCAAAATGCTAAATCATTTAAATCCGACGATGGGACATTATACGGAGCCAAGATTACAGGCGGAGGTTCTGGCGGAACGGTTTGTGTAGTCGGAAGGAATTCTCTGCAGAGCAGCCAACAAATTTTGGAGATTCAGCAGAGGTACAAAGATGCAACAGGGTATCTGCCATTTATTTTCGAGGGCTCGTCGCCCGGTGCCGGAAAATTCGGGTATCTAAGAATACGCCGCCGCGTCTCCCTCGATCCCAATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0005975 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009506 GO:0009702 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0030054 GO:0044237 GO:0044238 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046835 GO:0055044 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

GHMP_kinases_C GHMP_kinases_N GalKase_gal_bdg Glyco_trans_1_3

Protein Analysis

991

Amino Acids

108.66

Weight (kDa)

5.72

Isoelectric Point (pI)

43.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GalKase_gal_bdg	PF10509	492 - 533	3.5e-07	Galactokinase galactose-binding signature
GHMP_kinases_N	PF00288	610 - 698	3.8e-17	GHMP kinases N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G42850 AT3G42850 AT4G16130
fragaria_vesca	FvH4_2g36460 FvH4_2g36460 FvH4_2g36460
malus_domestica	MD08G1124400.v1.1 MD15G1103100.v1.1
prunus_persica	Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1 Prupe.1G456000_v2.0.a1
pyrus_communis	pycom08g10310 pycom15g09400
rosa_chinensis	RchiOBHm_Chr6g0305421
rosa_laevigata	RLG00000010892
rosa_multiflora	Rmu_sc0002782.1_g000020
rosa_roxburghii	Rroxscaffold_7G00162820
rosa_rugosa	Rorug06G0340200
rosa_samantha	Rh6AG452200 Rh6BG449500 Rh6CG466000 Rh6DG452700
rosa_wichuraiana	Rw6G039400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1826, 2542
AasI	GACNNNNNNGTC	1 cut(s)	368
Acc36I	ACCTGC	2 cut(s)	1826, 2542
AccB1I	GGYRCC	5 cut(s)	117, 304, 1204, 2240, 2912
AccB7I	CCANNNNNTGG	1 cut(s)	2032
AccII	CGCG	2 cut(s)	115, 2949
AclI	AACGTT	1 cut(s)	1724
AclWI	GGATC	8 cut(s)	621, 1276, 1743, 1799, 2233, 2480, 2498, 2954
AcoI	YGGCCR	3 cut(s)	85, 133, 2161
AcsI	RAATTY	9 cut(s)	417, 793, 1482, 2154, 2557, 2599, 2815, 2838, 2921
AcuI	CTGAAG	3 cut(s)	1122, 1341, 2385
AcyI	GRCGYC	3 cut(s)	35, 305, 1205
AfaI	GTAC	5 cut(s)	600, 921, 1872, 2700, 2861
AfiI	CCNNNNNNNGG	9 cut(s)	637, 1280, 1641, 1648, 1818, 1819, 2032, 2748, 2915
AgeI	ACCGGT	1 cut(s)	71
AhdI	GACNNNNNGTC	1 cut(s)	2204
AhlI	ACTAGT	1 cut(s)	676
AjiI	CACGTC	1 cut(s)	148
AjuI	GAANNNNNNNTTGG	4 cut(s)	1242, 1248, 1274, 1280
AleI	CACNNNNGTG	1 cut(s)	98
Alw21I	GWGCWC	2 cut(s)	163, 1501
Alw26I	GTCTC	7 cut(s)	287, 376, 1106, 1693, 1936, 2432, 2956
AlwI	GGATC	8 cut(s)	621, 1276, 1743, 1799, 2233, 2480, 2498, 2954
AlwNI	CAGNNNCTG	2 cut(s)	407, 845
Ama87I	CYCGRG	2 cut(s)	96, 309
AoxI	GGCC	5 cut(s)	85, 133, 1638, 1650, 2161
ApoI	RAATTY	9 cut(s)	417, 793, 1482, 2154, 2557, 2599, 2815, 2838, 2921
AsiGI	ACCGGT	1 cut(s)	71
Asp700I	GAANNNNTTC	3 cut(s)	2185, 2457, 2815
AspLEI	GCGC	5 cut(s)	115, 307, 1207, 1437, 1446
AspS9I	GGNCC	3 cut(s)	1639, 1651, 2071
AsuC2I	CCSGG	4 cut(s)	1439, 1642, 2564, 2910
AsuHPI	GGTGA	9 cut(s)	61, 145, 652, 1097, 1263, 2336, 2388, 2462, 2523
AsuII	TTCGAA	1 cut(s)	2233
AvaI	CYCGRG	2 cut(s)	96, 309
AvaII	GGWCC	1 cut(s)	2071
BalI	TGGCCA	1 cut(s)	2163
BanI	GGYRCC	5 cut(s)	117, 304, 1204, 2240, 2912
BanII	GRGCYC	3 cut(s)	163, 2056, 2903
BbsI	GAAGAC	1 cut(s)	166
Bbv12I	GWGCWC	2 cut(s)	163, 1501
BbvCI	CCTCAGC	1 cut(s)	2133
BccI	CCATC	8 cut(s)	359, 738, 796, 1748, 1816, 2062, 2336, 2741
BceAI	ACGGC	4 cut(s)	381, 889, 1131, 1895
BcgI	CGANNNNNNTGC	2 cut(s)	251, 285
BciVI	GTATCC	2 cut(s)	554, 863
BclI	TGATCA	2 cut(s)	2476, 2608
BcnI	CCSGG	4 cut(s)	1439, 1642, 2564, 2910
BcoDI	GTCTC	7 cut(s)	287, 376, 1106, 1693, 1936, 2432, 2956
BcuI	ACTAGT	1 cut(s)	676
BfaI	CTAG	8 cut(s)	365, 605, 677, 1397, 1494, 1550, 1865, 2538
BfmI	CTRYAG	4 cut(s)	1452, 2650, 2656, 2822
BfoI	RGCGCY	3 cut(s)	308, 1208, 1438
BfuAI	ACCTGC	2 cut(s)	1826, 2542
BfuI	GTATCC	2 cut(s)	554, 863
BglII	AGATCT	2 cut(s)	1345, 2008
Bme18I	GGWCC	1 cut(s)	2071
BmeRI	GACNNNNNGTC	1 cut(s)	2204
BmeT110I	CYCGRG	2 cut(s)	96, 309
BmgBI	CACGTC	1 cut(s)	148
BmgT120I	GGNCC	3 cut(s)	1639, 1651, 2071
BmrI	ACTGGG	1 cut(s)	1480
BmuI	ACTGGG	1 cut(s)	1480
BpiI	GAAGAC	1 cut(s)	166
BplI	GAGNNNNNCTC	2 cut(s)	874, 906
Bpu10I	CCTNAGC	2 cut(s)	277, 2133
Bpu14I	TTCGAA	1 cut(s)	2233
BpuEI	CTTGAG	4 cut(s)	973, 986, 1055, 2483
BpuMI	CCSGG	4 cut(s)	1439, 1642, 2564, 2910
BsaAI	YACGTR	1 cut(s)	2521
BsaHI	GRCGYC	3 cut(s)	35, 305, 1205
BsaI	GGTCTC	1 cut(s)	376
BsaJI	CCNNGG	6 cut(s)	308, 556, 777, 1635, 1654, 1984
BsaWI	WCCGGW	2 cut(s)	71, 155
BsaXI	ACNNNNNCTCC	4 cut(s)	145, 175, 654, 684
Bsc4I	CCNNNNNNNGG	9 cut(s)	637, 1280, 1641, 1648, 1818, 1819, 2032, 2748, 2915
Bse118I	RCCGGY	4 cut(s)	71, 131, 2292, 2691
Bse1I	ACTGG	4 cut(s)	810, 1018, 1475, 2489
Bse3DI	GCAATG	1 cut(s)	2121
BseDI	CCNNGG	6 cut(s)	308, 556, 777, 1635, 1654, 1984
BseLI	CCNNNNNNNGG	9 cut(s)	637, 1280, 1641, 1648, 1818, 1819, 2032, 2748, 2915
BseMI	GCAATG	1 cut(s)	2121
BseMII	CTCAG	4 cut(s)	387, 1419, 2124, 2427
BseNI	ACTGG	4 cut(s)	810, 1018, 1475, 2489
BseRI	GAGGAG	2 cut(s)	628, 662
BsgI	GTGCAG	3 cut(s)	420, 2235, 2292
Bsh1236I	CGCG	2 cut(s)	115, 2949
Bsh1285I	CGRYCG	1 cut(s)	265
BshFI	GGCC	5 cut(s)	87, 135, 1640, 1652, 2163
BshNI	GGYRCC	5 cut(s)	117, 304, 1204, 2240, 2912
BshTI	ACCGGT	1 cut(s)	71
BsiEI	CGRYCG	1 cut(s)	265
BsiHKAI	GWGCWC	2 cut(s)	163, 1501
BsiHKCI	CYCGRG	2 cut(s)	96, 309
BslFI	GGGAC	6 cut(s)	464, 1016, 1898, 1986, 2329, 2764
BslI	CCNNNNNNNGG	9 cut(s)	637, 1280, 1641, 1648, 1818, 1819, 2032, 2748, 2915
BsmAI	GTCTC	7 cut(s)	287, 376, 1106, 1693, 1936, 2432, 2956
BsmBI	CGTCTC	2 cut(s)	1106, 2956
BsmFI	GGGAC	6 cut(s)	464, 1016, 1898, 1986, 2329, 2764
BsmI	GAATGC	1 cut(s)	1786
BsnI	GGCC	5 cut(s)	87, 135, 1640, 1652, 2163
Bso31I	GGTCTC	1 cut(s)	376
BsoBI	CYCGRG	2 cut(s)	96, 309
Bsp119I	TTCGAA	1 cut(s)	2233
Bsp1286I	GDGCHC	4 cut(s)	163, 1501, 2056, 2903
Bsp19I	CCATGG	1 cut(s)	1984
BspANI	GGCC	5 cut(s)	87, 135, 1640, 1652, 2163
BspCNI	CTCAG	4 cut(s)	386, 1420, 2125, 2428
BspFNI	CGCG	2 cut(s)	115, 2949
BspMAI	CTGCAG	2 cut(s)	1456, 2826
BspMI	ACCTGC	2 cut(s)	1826, 2542
BspPI	GGATC	8 cut(s)	621, 1276, 1743, 1799, 2233, 2480, 2498, 2954
BspQI	GCTCTTC	1 cut(s)	1376
BspT104I	TTCGAA	1 cut(s)	2233
BspT107I	GGYRCC	5 cut(s)	117, 304, 1204, 2240, 2912
BspTNI	GGTCTC	1 cut(s)	376
BsrDI	GCAATG	1 cut(s)	2121
BsrFI	RCCGGY	4 cut(s)	71, 131, 2292, 2691
BsrI	ACTGG	4 cut(s)	810, 1018, 1475, 2489
BssAI	RCCGGY	4 cut(s)	71, 131, 2292, 2691
BssECI	CCNNGG	6 cut(s)	308, 556, 777, 1635, 1654, 1984
BssNI	GRCGYC	3 cut(s)	35, 305, 1205
BssT1I	CCWWGG	2 cut(s)	1654, 1984
Bst6I	CTCTTC	7 cut(s)	9, 209, 720, 1141, 1376, 1469, 1939
BstACI	GRCGYC	3 cut(s)	35, 305, 1205
BstAPI	GCANNNNNTGC	4 cut(s)	398, 815, 1832, 2716
BstBAI	YACGTR	1 cut(s)	2521
BstBI	TTCGAA	1 cut(s)	2233
BstDEI	CTNAG	8 cut(s)	277, 282, 373, 1148, 1428, 2133, 2436, 2933
BstDSI	CCRYGG	1 cut(s)	1984
BstFNI	CGCG	2 cut(s)	115, 2949
BstH2I	RGCGCY	3 cut(s)	308, 1208, 1438
BstHHI	GCGC	5 cut(s)	115, 307, 1207, 1437, 1446
BstMAI	GTCTC	7 cut(s)	287, 376, 1106, 1693, 1936, 2432, 2956
BstMCI	CGRYCG	1 cut(s)	265
BstNSI	RCATGY	3 cut(s)	396, 1634, 2091
BstSFI	CTRYAG	4 cut(s)	1452, 2650, 2656, 2822
BstSNI	TACGTA	1 cut(s)	2521
BstUI	CGCG	2 cut(s)	115, 2949
BstV2I	GAAGAC	1 cut(s)	166
BstX2I	RGATCY	6 cut(s)	626, 1345, 1735, 1804, 2008, 2225
BstXI	CCANNNNNNTGG	2 cut(s)	100, 711
BstYI	RGATCY	6 cut(s)	626, 1345, 1735, 1804, 2008, 2225
BsuI	GTATCC	2 cut(s)	554, 863
BsuRI	GGCC	5 cut(s)	87, 135, 1640, 1652, 2163
BtgI	CCRYGG	1 cut(s)	1984
BtgZI	GCGATG	1 cut(s)	2729
BtrI	CACGTC	1 cut(s)	148
BtsI	GCAGTG	2 cut(s)	532, 2582
BtsIMutI	CAGTG	7 cut(s)	532, 876, 1011, 1317, 2209, 2582, 2648
BveI	ACCTGC	2 cut(s)	1826, 2542
CaiI	CAGNNNCTG	2 cut(s)	407, 845
CfoI	GCGC	5 cut(s)	115, 307, 1207, 1437, 1446
Cfr10I	RCCGGY	4 cut(s)	71, 131, 2292, 2691
Cfr13I	GGNCC	3 cut(s)	1639, 1651, 2071
CseI	GACGC	2 cut(s)	24, 2938
CsiI	ACCWGGT	1 cut(s)	48
Csp6I	GTAC	5 cut(s)	599, 920, 1871, 2699, 2860
CspAI	ACCGGT	1 cut(s)	71
CspCI	CAANNNNNGTGG	2 cut(s)	676, 711
CviQI	GTAC	5 cut(s)	599, 920, 1871, 2699, 2860
DdeI	CTNAG	8 cut(s)	277, 282, 373, 1148, 1428, 2133, 2436, 2933
DinI	GGCGCC	2 cut(s)	306, 1206
DraI	TTTAAA	1 cut(s)	2737
DrdI	GACNNNNNNGTC	1 cut(s)	368
DriI	GACNNNNNGTC	1 cut(s)	2204
DseDI	GACNNNNNNGTC	1 cut(s)	368
EaeI	YGGCCR	3 cut(s)	85, 133, 2161
Eam1104I	CTCTTC	7 cut(s)	9, 209, 720, 1141, 1376, 1469, 1939
Eam1105I	GACNNNNNGTC	1 cut(s)	2204
EarI	CTCTTC	7 cut(s)	9, 209, 720, 1141, 1376, 1469, 1939
EciI	GGCGGA	5 cut(s)	539, 1406, 1897, 2793, 2805
Ecl136II	GAGCTC	1 cut(s)	161
Eco105I	TACGTA	1 cut(s)	2521
Eco130I	CCWWGG	2 cut(s)	1654, 1984
Eco24I	GRGCYC	3 cut(s)	163, 2056, 2903
Eco31I	GGTCTC	1 cut(s)	376
Eco47I	GGWCC	1 cut(s)	2071
Eco53kI	GAGCTC	1 cut(s)	161
Eco57I	CTGAAG	3 cut(s)	1122, 1341, 2385
Eco88I	CYCGRG	2 cut(s)	96, 309
EcoICRI	GAGCTC	1 cut(s)	161
EcoRI	GAATTC	2 cut(s)	417, 2815
EcoT14I	CCWWGG	2 cut(s)	1654, 1984
EcoT38I	GRGCYC	3 cut(s)	163, 2056, 2903
EgeI	GGCGCC	2 cut(s)	306, 1206
EheI	GGCGCC	2 cut(s)	306, 1206
ErhI	CCWWGG	2 cut(s)	1654, 1984
Esp3I	CGTCTC	2 cut(s)	1106, 2956
FalI	AAGNNNNNCTT	2 cut(s)	1778, 1810
FaqI	GGGAC	6 cut(s)	464, 1016, 1898, 1986, 2329, 2764
FauI	CCCGC	1 cut(s)	638
FauNDI	CATATG	2 cut(s)	1693, 1774
FbaI	TGATCA	2 cut(s)	2476, 2608
FriOI	GRGCYC	3 cut(s)	163, 2056, 2903
FspBI	CTAG	8 cut(s)	365, 605, 677, 1397, 1494, 1550, 1865, 2538
GlaI	GCGC	5 cut(s)	114, 306, 1206, 1436, 1445
HaeII	RGCGCY	3 cut(s)	308, 1208, 1438
HaeIII	GGCC	5 cut(s)	87, 135, 1640, 1652, 2163
HgaI	GACGC	2 cut(s)	24, 2938
HhaI	GCGC	5 cut(s)	115, 307, 1207, 1437, 1446
Hin1I	GRCGYC	3 cut(s)	35, 305, 1205
Hin6I	GCGC	5 cut(s)	113, 305, 1205, 1435, 1444
HinP1I	GCGC	5 cut(s)	113, 305, 1205, 1435, 1444
HindIII	AAGCTT	1 cut(s)	2105
HphI	GGTGA	9 cut(s)	61, 145, 652, 1097, 1263, 2336, 2388, 2462, 2523
Hpy166II	GTNNAC	5 cut(s)	145, 698, 1256, 1613, 1871
Hpy8I	GTNNAC	5 cut(s)	145, 698, 1256, 1613, 1871
Hpy99I	CGWCG	4 cut(s)	152, 2684, 2747, 2908
HpyAV	CCTTC	5 cut(s)	17, 715, 1061, 1913, 2805
HpyCH4IV	ACGT	6 cut(s)	66, 147, 1515, 1724, 1873, 2520
HpyF3I	CTNAG	8 cut(s)	277, 282, 373, 1148, 1428, 2133, 2436, 2933
HpySE526I	ACGT	6 cut(s)	66, 147, 1515, 1724, 1873, 2520
Hsp92I	GRCGYC	3 cut(s)	35, 305, 1205
HspAI	GCGC	5 cut(s)	113, 305, 1205, 1435, 1444
KasI	GGCGCC	2 cut(s)	304, 1204
KroI	GCCGGC	1 cut(s)	131
KroNI	GCCGGC	1 cut(s)	133
Ksp22I	TGATCA	2 cut(s)	2476, 2608
LguI	GCTCTTC	1 cut(s)	1376
LmnI	GCTCC	9 cut(s)	158, 166, 233, 649, 1304, 1441, 2051, 2681, 2762
MabI	ACCWGGT	1 cut(s)	48
MaeI	CTAG	8 cut(s)	365, 605, 677, 1397, 1494, 1550, 1865, 2538
MaeII	ACGT	6 cut(s)	66, 147, 1515, 1724, 1873, 2520
MaeIII	GTNAC	8 cut(s)	67, 74, 151, 433, 903, 2394, 2491, 2513
MfeI	CAATTG	1 cut(s)	2310
MflI	RGATCY	6 cut(s)	626, 1345, 1735, 1804, 2008, 2225
MhlI	GDGCHC	4 cut(s)	163, 1501, 2056, 2903
MlsI	TGGCCA	1 cut(s)	2163
MluNI	TGGCCA	1 cut(s)	2163
Mly113I	GGCGCC	2 cut(s)	305, 1205
MlyI	GAGTC	2 cut(s)	1406, 1455
MmeI	TCCRAC	7 cut(s)	2029, 2188, 2234, 2313, 2703, 2766, 2788
Mox20I	TGGCCA	1 cut(s)	2163
MroNI	GCCGGC	1 cut(s)	131
MroXI	GAANNNNTTC	3 cut(s)	2185, 2457, 2815
MscI	TGGCCA	1 cut(s)	2163
MseI	TTAA	8 cut(s)	254, 1706, 1853, 1991, 2375, 2424, 2568, 2736
MslI	CAYNNNNRTG	3 cut(s)	98, 2086, 2406
Msp20I	TGGCCA	1 cut(s)	2163
MspA1I	CMGCKG	3 cut(s)	299, 407, 1457
MunI	CAATTG	1 cut(s)	2310
Mva1269I	GAATGC	1 cut(s)	1786
MvnI	CGCG	2 cut(s)	115, 2949
NaeI	GCCGGC	1 cut(s)	133
NarI	GGCGCC	2 cut(s)	305, 1205
NciI	CCSGG	4 cut(s)	1439, 1642, 2564, 2910
NcoI	CCATGG	1 cut(s)	1984
NdeI	CATATG	2 cut(s)	1693, 1774
NgoMIV	GCCGGC	1 cut(s)	131
NmuCI	GTSAC	5 cut(s)	67, 74, 151, 433, 2394
NspI	RCATGY	3 cut(s)	396, 1634, 2091
NspV	TTCGAA	1 cut(s)	2233
OliI	CACNNNNGTG	1 cut(s)	98
PaeI	GCATGC	1 cut(s)	396
PaqCI	CACCTGC	2 cut(s)	1826, 2542
PciSI	GCTCTTC	1 cut(s)	1376
PcsI	WCGNNNNNNNCGW	3 cut(s)	18, 135, 144
PctI	GAATGC	1 cut(s)	1786
PdiI	GCCGGC	1 cut(s)	133
PdmI	GAANNNNTTC	3 cut(s)	2185, 2457, 2815
PfeI	GAWTC	8 cut(s)	81, 124, 1333, 1400, 1600, 2038, 2186, 2848
PflFI	GACNNNGTC	1 cut(s)	170
PflMI	CCANNNNNTGG	1 cut(s)	2032
PfoI	TCCNGGA	2 cut(s)	38, 739
PinAI	ACCGGT	1 cut(s)	71
Ple19I	CGATCG	1 cut(s)	265
PleI	GAGTC	2 cut(s)	1406, 1455
PluTI	GGCGCC	2 cut(s)	308, 1208
PpsI	GAGTC	2 cut(s)	1406, 1455
Ppu21I	YACGTR	1 cut(s)	2521
Psp124BI	GAGCTC	1 cut(s)	163
Psp1406I	AACGTT	1 cut(s)	1724
PspPI	GGNCC	3 cut(s)	1639, 1651, 2071
PsrI	GAACNNNNNNTAC	4 cut(s)	481, 513, 1854, 1886
PstI	CTGCAG	2 cut(s)	1456, 2826
PstNI	CAGNNNCTG	2 cut(s)	407, 845
PsuI	RGATCY	6 cut(s)	626, 1345, 1735, 1804, 2008, 2225
PsyI	GACNNNGTC	1 cut(s)	170
PvuI	CGATCG	1 cut(s)	265
PvuII	CAGCTG	2 cut(s)	299, 1457
RsaI	GTAC	5 cut(s)	600, 921, 1872, 2700, 2861
RsaNI	GTAC	5 cut(s)	599, 920, 1871, 2699, 2860
RseI	CAYNNNNRTG	3 cut(s)	98, 2086, 2406
SacI	GAGCTC	1 cut(s)	163
SapI	GCTCTTC	1 cut(s)	1376
SaqAI	TTAA	8 cut(s)	254, 1706, 1853, 1991, 2375, 2424, 2568, 2736
Sau96I	GGNCC	3 cut(s)	1639, 1651, 2071
SchI	GAGTC	2 cut(s)	1406, 1455
SduI	GDGCHC	4 cut(s)	163, 1501, 2056, 2903
SexAI	ACCWGGT	1 cut(s)	48
SfcI	CTRYAG	4 cut(s)	1452, 2650, 2656, 2822
SfoI	GGCGCC	2 cut(s)	306, 1206
SfuI	TTCGAA	1 cut(s)	2233
SinI	GGWCC	1 cut(s)	2071
SmiI	ATTTAAAT	1 cut(s)	2737
SmiMI	CAYNNNNRTG	3 cut(s)	98, 2086, 2406
SmlI	CTYRAG	4 cut(s)	952, 965, 1034, 2462
SmoI	CTYRAG	4 cut(s)	952, 965, 1034, 2462
SnaBI	TACGTA	1 cut(s)	2521
SpeI	ACTAGT	1 cut(s)	676
SphI	GCATGC	1 cut(s)	396
SspDI	GGCGCC	2 cut(s)	304, 1204
SspMI	CTAG	8 cut(s)	365, 605, 677, 1397, 1494, 1550, 1865, 2538
SstI	GAGCTC	1 cut(s)	163
StyI	CCWWGG	2 cut(s)	1654, 1984
SwaI	ATTTAAAT	1 cut(s)	2737
TaiI	ACGT	6 cut(s)	69, 150, 1518, 1727, 1876, 2523
TaqI	TCGA	5 cut(s)	105, 261, 2233, 2895, 2958
TauI	GCSGC	5 cut(s)	118, 407, 1074, 2946, 2949
TfiI	GAWTC	8 cut(s)	81, 124, 1333, 1400, 1600, 2038, 2186, 2848
Tru1I	TTAA	8 cut(s)	254, 1706, 1853, 1991, 2375, 2424, 2568, 2736
Tru9I	TTAA	8 cut(s)	254, 1706, 1853, 1991, 2375, 2424, 2568, 2736
TscAI	CASTG	7 cut(s)	539, 883, 1018, 1324, 2209, 2589, 2648
TseFI	GTSAC	5 cut(s)	67, 74, 151, 433, 2394
Tsp45I	GTSAC	5 cut(s)	67, 74, 151, 433, 2394
TspDTI	ATGAA	9 cut(s)	196, 445, 787, 885, 954, 1080, 1328, 1340, 2568
TspGWI	ACGGA	6 cut(s)	93, 344, 572, 1612, 2697, 2775
TspRI	CASTG	7 cut(s)	539, 883, 1018, 1324, 2209, 2589, 2648
Tth111I	GACNNNGTC	1 cut(s)	170
Van91I	CCANNNNNTGG	1 cut(s)	2032
VpaK11BI	GGWCC	1 cut(s)	2071
XapI	RAATTY	9 cut(s)	417, 793, 1482, 2154, 2557, 2599, 2815, 2838, 2921
XceI	RCATGY	3 cut(s)	396, 1634, 2091
XcmI	CCANNNNNNNNNTGG	2 cut(s)	46, 1815
XmnI	GAANNNNTTC	3 cut(s)	2185, 2457, 2815
XspI	CTAG	8 cut(s)	365, 605, 677, 1397, 1494, 1550, 1865, 2538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.