FvH4_2g38060

cinnamyl alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012582
Physical Location & Seq
Reverse (-)
27578887 .. 27581862
2976 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38060.t2

Sequence Viewer

Length: 1113 bp
ATGGCTCAGACAACTCCGAATCACATACAGACCGTTTCTGGGTGGGCAGCTCATGATTCCTCCGGCAAGATTACTCCTTTCATCTTCAAACGAAGAGAAAACGGGATCAACGATGTTACGATAAAGATATTGTACTGTGGCATATGCCACACTGATCTTCACCAAGCTAGGAATGACTGGGGTATCACCATGTATCCTATTGTCCCTGGTCATGAGATTACTGGGTTGGTCATGAAAGTTGGGAGCAACGTCAAGAACTTCTCGGTCGGAGATAGAGTCGGTGTCGGGTGCTTGGCGGCGACGTGTTTGGAGTGTGATTTCTGCAAGGAATCCCAGGAGAACTACTGCGAGGACCTTCAGTTTACCTACAATGGCGTCTTCTGGGATGGTAGTATTACCTACGGTGGATACTCCAAAATGTTGGTCGCCGATCATAGGTACGTGGTGCGCATACCAGAAAACCTACCGATGGATTCTGCAGCGCCGTTATTGTGCGCCGGAGTGACAGTGTTCACCCCCTTCAGAGACCACGACCTACACAAAACACCGGGAAAGAAGATCGGCGTCGTCGGCCTCGGAGGTCTCGGACACGTCGCGGTGAAATTCGGAAAGGCCTTTGGTCATCACGTCACCGTCATCAGCACATCTCCTTCCAAAGAGGAAGAGGCCAAAGGTCACTTGGGTGCAGACGATTTCATAGTCAGCACTGATGAGCAACAAATGCAGAGAGGAAAGAGGAGCCTGGACTTCATACTAAACACGGTGTCAGCTAAGCACTGTCTCGGACCACTGTTAGAGTTGCTCAAAGTGAACGGGACCATGGTGGTTGTGGGTGCACCTGACCAGCCTTTTACCTTGCCTTCATTCCCTCTGATATTTGGGAAGAGAGCAGTGAAGGGCAGTGTGATCGGAGGGATGGAGGAGACGAGGGAGATGATGGAACTTTGCGGCAAACACAACATTACATGCGACATTGAGCTTACAACGCCGGACAAGATCAACCAAGCCTTCGAACGCGTCGCGAAGAATGACGTTAGGTATCGCTTTGTTATCGACATTGCCAGTTCCCCAGCTGTTGTTGTTGATGATGATCAAATCTATTCCAGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

371

Amino Acids

40.54

Weight (kDa)

6.16

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 2 - 90 9.7e-18 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 131 - 252 2e-21 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 449
AccII CGCG 3 cut(s) 596, 1017, 1022
AciI CCGC 3 cut(s) 296, 596, 948
AclWI GGATC 1 cut(s) 113
AcsI RAATTY 1 cut(s) 602
AcuI CTGAAG 2 cut(s) 341, 505
AcyI GRCGYC 2 cut(s) 375, 564
AfaI GTAC 2 cut(s) 134, 440
AfiI CCNNNNNNNGG 3 cut(s) 39, 435, 469
AflIII ACRYGT 3 cut(s) 302, 589, 1015
AgsI TTSAA 1 cut(s) 88
AjiI CACGTC 3 cut(s) 303, 592, 628
AjnI CCWGG 3 cut(s) 205, 333, 741
AleI CACNNNNGTG 1 cut(s) 681
AluBI AGCT 5 cut(s) 50, 167, 770, 979, 1073
AluI AGCT 5 cut(s) 50, 167, 770, 979, 1073
Alw21I GWGCWC 1 cut(s) 838
Alw26I GTCTC 4 cut(s) 519, 587, 785, 917
Alw44I GTGCAC 1 cut(s) 834
AlwI GGATC 1 cut(s) 113
AoxI GGCC 3 cut(s) 571, 612, 666
ApaLI GTGCAC 1 cut(s) 834
ApeKI GCWGC 2 cut(s) 47, 479
ApoI RAATTY 1 cut(s) 602
ArsI GACNNNNNNTTYG 2 cut(s) 408, 440
AspLEI GCGC 3 cut(s) 450, 484, 497
AspS9I GGNCC 3 cut(s) 352, 785, 816
AsuC2I CCSGG 1 cut(s) 549
AsuHPI GGTGA 5 cut(s) 152, 178, 505, 610, 622
AsuII TTCGAA 1 cut(s) 1011
AvaII GGWCC 3 cut(s) 352, 785, 816
BaeGI GKGCMC 1 cut(s) 838
BaeI ACNNNNGTAYC 2 cut(s) 166, 199
BbsI GAAGAC 1 cut(s) 370
Bbv12I GWGCWC 1 cut(s) 838
BbvI GCAGC 2 cut(s) 59, 491
BccI CCATC 4 cut(s) 380, 463, 910, 931
BceAI ACGGC 1 cut(s) 469
BcgI CGANNNNNNTGC 2 cut(s) 889, 923
BciT130I CCWGG 3 cut(s) 207, 335, 743
BciVI GTATCC 2 cut(s) 204, 401
BclI TGATCA 1 cut(s) 1090
BcnI CCSGG 1 cut(s) 549
BcoDI GTCTC 4 cut(s) 519, 587, 785, 917
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 477
BfoI RGCGCY 1 cut(s) 485
BfuI GTATCC 2 cut(s) 204, 401
BisI GCNGC 4 cut(s) 48, 297, 480, 949
BlpI GCTNAGC 1 cut(s) 771
BlsI GCNGC 4 cut(s) 49, 298, 481, 950
Bme1390I CCNGG 4 cut(s) 207, 335, 549, 743
Bme18I GGWCC 3 cut(s) 352, 785, 816
BmgBI CACGTC 3 cut(s) 303, 592, 628
BmgT120I GGNCC 3 cut(s) 352, 785, 816
BmiI GGNNCC 2 cut(s) 740, 817
BmrFI CCNGG 4 cut(s) 207, 335, 549, 743
BmrI ACTGGG 2 cut(s) 187, 231
BmuI ACTGGG 2 cut(s) 187, 231
BpiI GAAGAC 1 cut(s) 370
Bpu1102I GCTNAGC 1 cut(s) 771
Bpu14I TTCGAA 1 cut(s) 1011
BpuMI CCSGG 1 cut(s) 549
BsaAI YACGTR 1 cut(s) 442
BsaBI GATNNNNATC 1 cut(s) 1089
BsaHI GRCGYC 2 cut(s) 375, 564
BsaI GGTCTC 2 cut(s) 519, 587
BsaJI CCNNGG 4 cut(s) 205, 333, 574, 819
BsaXI ACNNNNNCTCC 4 cut(s) 261, 291, 492, 522
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 435, 469
Bse1I ACTGG 4 cut(s) 182, 226, 1062, 1104
Bse3DI GCAATG 1 cut(s) 1056
Bse8I GATNNNNATC 1 cut(s) 1089
BseBI CCWGG 3 cut(s) 207, 335, 743
BseDI CCNNGG 4 cut(s) 205, 333, 574, 819
BseGI GGATG 2 cut(s) 391, 921
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 3 cut(s) 39, 435, 469
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 4 cut(s) 182, 226, 1062, 1104
BseRI GAGGAG 2 cut(s) 751, 935
BseSI GKGCMC 1 cut(s) 838
BseXI GCAGC 2 cut(s) 59, 491
BseYI CCCAGC 1 cut(s) 1069
BsgI GTGCAG 1 cut(s) 705
Bsh1236I CGCG 3 cut(s) 596, 1017, 1022
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 3 cut(s) 573, 614, 668
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 838
BsiSI CCGG 4 cut(s) 63, 498, 548, 989
BslFI GGGAC 2 cut(s) 188, 829
BslI CCNNNNNNNGG 3 cut(s) 39, 435, 469
BsmAI GTCTC 4 cut(s) 519, 587, 785, 917
BsmBI CGTCTC 1 cut(s) 917
BsmFI GGGAC 2 cut(s) 188, 829
BsnI GGCC 3 cut(s) 573, 614, 668
Bso31I GGTCTC 2 cut(s) 519, 587
Bsp119I TTCGAA 1 cut(s) 1011
Bsp1286I GDGCHC 1 cut(s) 838
Bsp143I GATC 7 cut(s) 105, 154, 430, 558, 906, 996, 1090
Bsp1720I GCTNAGC 1 cut(s) 771
Bsp19I CCATGG 1 cut(s) 819
Bsp68I TCGCGA 1 cut(s) 1022
BspACI CCGC 3 cut(s) 296, 596, 948
BspANI GGCC 3 cut(s) 573, 614, 668
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 3 cut(s) 596, 1017, 1022
BspHI TCATGA 3 cut(s) 52, 211, 231
BspLI GGNNCC 2 cut(s) 740, 817
BspMAI CTGCAG 1 cut(s) 481
BspPI GGATC 1 cut(s) 113
BspT104I TTCGAA 1 cut(s) 1011
BspTNI GGTCTC 2 cut(s) 519, 587
BsrDI GCAATG 1 cut(s) 1056
BsrI ACTGG 4 cut(s) 182, 226, 1062, 1104
BssECI CCNNGG 4 cut(s) 205, 333, 574, 819
BssMI GATC 7 cut(s) 105, 154, 430, 558, 906, 996, 1090
BssNI GRCGYC 2 cut(s) 375, 564
BssT1I CCWWGG 1 cut(s) 819
Bst2UI CCWGG 3 cut(s) 207, 335, 743
Bst4CI ACNGT 8 cut(s) 34, 137, 404, 508, 634, 763, 779, 792
Bst6I CTCTTC 3 cut(s) 88, 657, 878
BstACI GRCGYC 2 cut(s) 375, 564
BstAPI GCANNNNNTGC 1 cut(s) 721
BstBAI YACGTR 1 cut(s) 442
BstBI TTCGAA 1 cut(s) 1011
BstDEI CTNAG 2 cut(s) 6, 771
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 2 cut(s) 391, 921
BstFNI CGCG 3 cut(s) 596, 1017, 1022
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 3 cut(s) 450, 484, 497
BstKTI GATC 7 cut(s) 108, 157, 433, 561, 909, 999, 1093
BstMAI GTCTC 4 cut(s) 519, 587, 785, 917
BstMBI GATC 7 cut(s) 105, 154, 430, 558, 906, 996, 1090
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 3 cut(s) 570, 721, 985
BstNI CCWGG 3 cut(s) 207, 335, 743
BstNSI RCATGY 1 cut(s) 969
BstSCI CCNGG 4 cut(s) 205, 333, 547, 741
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 838
BstUI CGCG 3 cut(s) 596, 1017, 1022
BstV1I GCAGC 2 cut(s) 59, 491
BstV2I GAAGAC 1 cut(s) 370
BstXI CCANNNNNNTGG 1 cut(s) 421
BsuI GTATCC 2 cut(s) 204, 401
BsuRI GGCC 3 cut(s) 573, 614, 668
BtgI CCRYGG 1 cut(s) 819
BtrI CACGTC 3 cut(s) 303, 592, 628
BtsCI GGATG 2 cut(s) 391, 921
BtsI GCAGTG 2 cut(s) 897, 907
BtsIMutI CAGTG 7 cut(s) 150, 513, 705, 775, 788, 897, 907
BtuMI TCGCGA 1 cut(s) 1022
CciI TCATGA 3 cut(s) 52, 211, 231
CfoI GCGC 3 cut(s) 450, 484, 497
Cfr13I GGNCC 3 cut(s) 352, 785, 816
CseI GACGC 3 cut(s) 364, 553, 1006
Csp6I GTAC 2 cut(s) 133, 439
CviAII CATG 6 cut(s) 53, 190, 212, 232, 820, 966
CviQI GTAC 2 cut(s) 133, 439
DdeI CTNAG 2 cut(s) 6, 771
DpnI GATC 7 cut(s) 107, 156, 432, 560, 908, 998, 1092
DpnII GATC 7 cut(s) 105, 154, 430, 558, 906, 996, 1090
Eam1104I CTCTTC 3 cut(s) 88, 657, 878
EarI CTCTTC 3 cut(s) 88, 657, 878
Eco130I CCWWGG 1 cut(s) 819
Eco147I AGGCCT 1 cut(s) 614
Eco31I GGTCTC 2 cut(s) 519, 587
Eco47I GGWCC 3 cut(s) 352, 785, 816
Eco57I CTGAAG 2 cut(s) 341, 505
EcoO109I RGGNCCY 1 cut(s) 352
EcoRII CCWGG 3 cut(s) 205, 333, 741
EcoT14I CCWWGG 1 cut(s) 819
ErhI CCWWGG 1 cut(s) 819
Esp3I CGTCTC 1 cut(s) 917
FaeI CATG 6 cut(s) 56, 193, 215, 235, 823, 969
FaqI GGGAC 2 cut(s) 188, 829
FatI CATG 6 cut(s) 52, 189, 211, 231, 819, 965
FauNDI CATATG 1 cut(s) 143
FbaI TGATCA 1 cut(s) 1090
Fnu4HI GCNGC 4 cut(s) 48, 297, 480, 949
FokI GGATG 2 cut(s) 398, 928
Fsp4HI GCNGC 4 cut(s) 48, 297, 480, 949
FspAI RTGCGCAY 1 cut(s) 449
FspBI CTAG 1 cut(s) 168
FspI TGCGCA 1 cut(s) 449
GlaI GCGC 3 cut(s) 449, 483, 496
GluI GCNGC 4 cut(s) 48, 297, 480, 949
GsaI CCCAGC 1 cut(s) 1073
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 3 cut(s) 573, 614, 668
HapII CCGG 4 cut(s) 63, 498, 548, 989
HgaI GACGC 3 cut(s) 364, 553, 1006
HhaI GCGC 3 cut(s) 450, 484, 497
Hin1I GRCGYC 2 cut(s) 375, 564
Hin1II CATG 6 cut(s) 56, 193, 215, 235, 823, 969
Hin6I GCGC 3 cut(s) 448, 482, 495
HinP1I GCGC 3 cut(s) 448, 482, 495
HinfI GANTC 5 cut(s) 19, 56, 276, 329, 473
HpaII CCGG 4 cut(s) 63, 498, 548, 989
HphI GGTGA 5 cut(s) 152, 178, 505, 610, 622
Hpy166II GTNNAC 4 cut(s) 363, 513, 811, 836
Hpy188III TCNNGA 5 cut(s) 53, 212, 232, 253, 1021
Hpy8I GTNNAC 4 cut(s) 363, 513, 811, 836
Hpy99I CGWCG 5 cut(s) 304, 569, 572, 596, 1022
HpyAV CCTTC 6 cut(s) 365, 529, 660, 870, 889, 1018
HpyCH4III ACNGT 8 cut(s) 34, 137, 404, 508, 634, 763, 779, 792
HpyCH4IV ACGT 6 cut(s) 249, 302, 441, 591, 627, 1032
HpyCH4V TGCA 5 cut(s) 324, 479, 686, 724, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 570, 721, 985
HpyF3I CTNAG 2 cut(s) 6, 771
HpySE526I ACGT 6 cut(s) 249, 302, 441, 591, 627, 1032
Hsp92I GRCGYC 2 cut(s) 375, 564
Hsp92II CATG 6 cut(s) 56, 193, 215, 235, 823, 969
HspAI GCGC 3 cut(s) 448, 482, 495
Ksp22I TGATCA 1 cut(s) 1090
Kzo9I GATC 7 cut(s) 105, 154, 430, 558, 906, 996, 1090
LmnI GCTCC 2 cut(s) 243, 738
Lsp1109I GCAGC 2 cut(s) 59, 491
MaeI CTAG 1 cut(s) 168
MaeII ACGT 6 cut(s) 249, 302, 441, 591, 627, 1032
MaeIII GTNAC 4 cut(s) 115, 502, 628, 674
MalI GATC 7 cut(s) 107, 156, 432, 560, 908, 998, 1092
MboI GATC 7 cut(s) 105, 154, 430, 558, 906, 996, 1090
MboII GAAGA 8 cut(s) 76, 105, 149, 370, 568, 674, 895, 1036
MhlI GDGCHC 1 cut(s) 838
MluCI AATT 1 cut(s) 602
MluI ACGCGT 1 cut(s) 1015
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 247
MslI CAYNNNNRTG 1 cut(s) 681
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 4 cut(s) 63, 498, 548, 989
MspR9I CCNGG 4 cut(s) 207, 335, 549, 743
MvaI CCWGG 3 cut(s) 207, 335, 743
MvnI CGCG 3 cut(s) 596, 1017, 1022
MwoI GCNNNNNNNGC 3 cut(s) 570, 721, 985
NciI CCSGG 1 cut(s) 549
NcoI CCATGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 143
NdeII GATC 7 cut(s) 105, 154, 430, 558, 906, 996, 1090
NlaIII CATG 6 cut(s) 56, 193, 215, 235, 823, 969
NlaIV GGNNCC 2 cut(s) 740, 817
NmuCI GTSAC 3 cut(s) 502, 628, 674
NruI TCGCGA 1 cut(s) 1022
NsbI TGCGCA 1 cut(s) 449
NspI RCATGY 1 cut(s) 969
NspV TTCGAA 1 cut(s) 1011
OliI CACNNNNGTG 1 cut(s) 681
PagI TCATGA 3 cut(s) 52, 211, 231
PceI AGGCCT 1 cut(s) 614
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 4 cut(s) 19, 56, 329, 473
PkrI GCNGC 4 cut(s) 49, 298, 481, 950
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
Ppu21I YACGTR 1 cut(s) 442
PpuMI RGGWCCY 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 3 cut(s) 205, 333, 741
PspFI CCCAGC 1 cut(s) 1069
PspGI CCWGG 3 cut(s) 205, 333, 741
PspN4I GGNNCC 2 cut(s) 740, 817
PspPI GGNCC 3 cut(s) 352, 785, 816
PspPPI RGGWCCY 1 cut(s) 352
PstI CTGCAG 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 1073
RruI TCGCGA 1 cut(s) 1022
RsaI GTAC 2 cut(s) 134, 440
RsaNI GTAC 2 cut(s) 133, 439
RseI CAYNNNNRTG 1 cut(s) 681
SatI GCNGC 4 cut(s) 48, 297, 480, 949
Sau3AI GATC 7 cut(s) 105, 154, 430, 558, 906, 996, 1090
Sau96I GGNCC 3 cut(s) 352, 785, 816
SchI GAGTC 1 cut(s) 285
ScrFI CCNGG 4 cut(s) 207, 335, 549, 743
SduI GDGCHC 1 cut(s) 838
SfcI CTRYAG 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 1011
SinI GGWCC 3 cut(s) 352, 785, 816
SmiMI CAYNNNNRTG 1 cut(s) 681
Sse9I AATT 1 cut(s) 602
SseBI AGGCCT 1 cut(s) 614
SsiI CCGC 3 cut(s) 296, 596, 948
SspMI CTAG 1 cut(s) 168
StuI AGGCCT 1 cut(s) 614
StyD4I CCNGG 4 cut(s) 205, 333, 547, 741
StyI CCWWGG 1 cut(s) 819
TaaI ACNGT 8 cut(s) 34, 137, 404, 508, 634, 763, 779, 792
TaiI ACGT 6 cut(s) 252, 305, 444, 594, 630, 1035
TaqI TCGA 2 cut(s) 1011, 1053
TaqII GACCGA 1 cut(s) 253
TasI AATT 1 cut(s) 602
TatI WGTACW 1 cut(s) 132
TauI GCSGC 2 cut(s) 299, 951
TfiI GAWTC 4 cut(s) 19, 56, 329, 473
TscAI CASTG 7 cut(s) 157, 513, 712, 782, 795, 897, 907
TseFI GTSAC 3 cut(s) 502, 628, 674
TseI GCWGC 2 cut(s) 47, 479
Tsp45I GTSAC 3 cut(s) 502, 628, 674
TspDTI ATGAA 5 cut(s) 70, 248, 685, 739, 852
TspRI CASTG 7 cut(s) 157, 513, 712, 782, 795, 897, 907
VneI GTGCAC 1 cut(s) 834
VpaK11BI GGWCC 3 cut(s) 352, 785, 816
XapI RAATTY 1 cut(s) 602
XceI RCATGY 1 cut(s) 969
XcmI CCANNNNNNNNNTGG 2 cut(s) 676, 826
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.