MD15G1008100.v1.1

cinnamyl alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012582
Physical Location & Seq
Reverse (-)
426690 .. 429407
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1008100.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGGCTCAGACGACTCCAAATCACACACAGACAGTTTCTGGATGGGCAGCTCACGATCCCTCCGGCAAGATTACTCCTTTCGTCTTCAAACGAAGAGAAAATGGGATCAACGATGTGACCATAAAGGTCTTGTACTGTGGGATATGCCACACTGATCTCCACTTTGCTAAGAATGACTGGGGCATGACCATGTATCCCGTAGTTCCCGGGCATGAGATTACAGGGCTGATCACGAAAGTTGGGAGCAACGTGAAGGAGTTCAAGGAAGGAGACAGAGTCGGGGTTGGGTGTTTGGCGGCGACATGTTTGGAGTGTGAGTTCTGCAAATCCTCCCAAGAGAACTACTGCGACCAGCTCCAGTTTACATACAACGGCATCTTCTGGGACGGCAGTATCACCTACGGTGGCTACTCCAAGATGTTGGTCGCAGATCACAGGTACGTGGTACACATACCAGAGAATCTGCCGTTGGATGCAACAGCGCCGCTATTGTGCGCGGGGGTGACGGTGTTCACCCCCATGAAAGACCACAAGCTCCACAAAACAACGGGGAAGAAGATCGGGGTGGTTGGCCTCGGAGGTCTGGGACACGTAGCAGTGAAGTTTGGGAAAGCCTTTGGTCACCATGTCACTGTCATCAGCACCTCTCCGGCCAAAGAGAAAGAGGCTAAAGAGCATTTGGGTGCCGACGACTTCCTTGTCAGCACTGATCCTCAACAACTGCAGAAAGGGAAGAGGACACTCGACTTCATATTAAATACAGTGTCCGCTAAGCACTCGCTTGGGTCCATCTTAGAACTGCTCAAAGTGAATGGAACTATGGTGGTTGTGGGTGCACCGGACCAGCCATTTGAATTGCCTTCATTTCCTCTGATATTTGGGAAGAGAGCAGTGAAGGGAAGTGTGATAGGAGGAATTAGTGAGACAAGGGAGATGATGAATTTATGCGGGAAGCACAACATAACGTGCGACATTGAGCTTACAACGCCGGACAAAATCAACGAGGCGTTGGACCGCGTTGCAAAGAATGATGTTAGGTACCGATTTGTCATTGACATTGCTGCTGCTGATGCTAAGGCTCGAATGCCTTCCAAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

367

Amino Acids

39.94

Weight (kDa)

8.24

Isoelectric Point (pI)

27.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 37 - 153 5.1e-27 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 194 - 315 1e-21 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 698
Acc65I GGTACC 1 cut(s) 1038
AccB1I GGYRCC 2 cut(s) 683, 1038
AccII CGCG 2 cut(s) 497, 1017
AciI CCGC 6 cut(s) 296, 485, 497, 768, 948, 1015
AclWI GGATC 3 cut(s) 50, 113, 704
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 1 cut(s) 940
AfaI GTAC 4 cut(s) 134, 440, 447, 1040
AflIII ACRYGT 2 cut(s) 302, 589
AgsI TTSAA 3 cut(s) 88, 262, 854
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
AluBI AGCT 4 cut(s) 50, 355, 535, 979
AluI AGCT 4 cut(s) 50, 355, 535, 979
Alw21I GWGCWC 1 cut(s) 838
Alw26I GTCTC 2 cut(s) 264, 917
Alw44I GTGCAC 1 cut(s) 834
AlwI GGATC 3 cut(s) 50, 113, 704
AlwNI CAGNNNCTG 1 cut(s) 38
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 2 cut(s) 571, 651
ApaLI GTGCAC 1 cut(s) 834
ApeKI GCWGC 3 cut(s) 47, 1061, 1064
ApoI RAATTY 1 cut(s) 940
ArsI GACNNNNNNTTYG 2 cut(s) 833, 865
Asp700I GAANNNNTTC 2 cut(s) 257, 1087
Asp718I GGTACC 1 cut(s) 1038
AspLEI GCGC 2 cut(s) 484, 497
AspS9I GGNCC 3 cut(s) 786, 841, 1012
AsuC2I CCSGG 2 cut(s) 207, 208
AsuHPI GGTGA 4 cut(s) 388, 505, 514, 614
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 3 cut(s) 786, 841, 1012
BaeGI GKGCMC 1 cut(s) 838
BaeI ACNNNNGTAYC 2 cut(s) 376, 409
BanI GGYRCC 2 cut(s) 683, 1038
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 838
BbvI GCAGC 3 cut(s) 59, 1048, 1051
BccI CCATC 2 cut(s) 36, 797
BceAI ACGGC 3 cut(s) 388, 403, 451
BciVI GTATCC 1 cut(s) 204
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 2 cut(s) 207, 208
BcoDI GTCTC 2 cut(s) 264, 917
BfmI CTRYAG 1 cut(s) 722
BfoI RGCGCY 1 cut(s) 485
BfuI GTATCC 1 cut(s) 204
BisI GCNGC 5 cut(s) 48, 297, 485, 1062, 1065
BlpI GCTNAGC 1 cut(s) 771
BlsI GCNGC 5 cut(s) 49, 298, 486, 1063, 1066
Bme1390I CCNGG 2 cut(s) 207, 208
Bme18I GGWCC 3 cut(s) 786, 841, 1012
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 3 cut(s) 786, 841, 1012
BmiI GGNNCC 3 cut(s) 685, 787, 1040
BmrFI CCNGG 2 cut(s) 207, 208
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 3 cut(s) 384, 463, 1060
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 76
BpmI CTGGAG 1 cut(s) 341
Bpu10I CCTNAGC 1 cut(s) 1074
Bpu1102I GCTNAGC 1 cut(s) 771
BpuMI CCSGG 2 cut(s) 207, 208
BsaAI YACGTR 2 cut(s) 442, 592
BsaJI CCNNGG 2 cut(s) 206, 574
BsaWI WCCGGW 1 cut(s) 838
BsaXI ACNNNNNCTCC 6 cut(s) 44, 74, 261, 291, 302, 332
Bse1I ACTGG 2 cut(s) 182, 358
Bse3DI GCAATG 1 cut(s) 1056
BseDI CCNNGG 2 cut(s) 206, 574
BseGI GGATG 2 cut(s) 47, 478
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 2 cut(s) 182, 358
BseSI GKGCMC 1 cut(s) 838
BseXI GCAGC 3 cut(s) 59, 1048, 1051
Bsh1236I CGCG 2 cut(s) 497, 1017
BshFI GGCC 2 cut(s) 573, 653
BshNI GGYRCC 2 cut(s) 683, 1038
BsiHKAI GWGCWC 1 cut(s) 838
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 5 cut(s) 63, 207, 650, 839, 989
BslFI GGGAC 2 cut(s) 398, 600
BsmAI GTCTC 2 cut(s) 264, 917
BsmFI GGGAC 2 cut(s) 398, 600
BsmI GAATGC 1 cut(s) 1089
BsnI GGCC 2 cut(s) 573, 653
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 838
Bsp143I GATC 7 cut(s) 55, 105, 154, 228, 430, 558, 709
Bsp1720I GCTNAGC 1 cut(s) 771
BspACI CCGC 6 cut(s) 296, 485, 497, 768, 948, 1015
BspANI GGCC 2 cut(s) 573, 653
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 2 cut(s) 497, 1017
BspLI GGNNCC 3 cut(s) 685, 787, 1040
BspMAI CTGCAG 1 cut(s) 726
BspPI GGATC 3 cut(s) 50, 113, 704
BspT107I GGYRCC 2 cut(s) 683, 1038
BsrDI GCAATG 1 cut(s) 1056
BsrI ACTGG 2 cut(s) 182, 358
BssECI CCNNGG 2 cut(s) 206, 574
BssMI GATC 7 cut(s) 55, 105, 154, 228, 430, 558, 709
Bst4CI ACNGT 6 cut(s) 34, 137, 404, 508, 634, 763
Bst6I CTCTTC 3 cut(s) 88, 728, 878
BstBAI YACGTR 2 cut(s) 442, 592
BstDEI CTNAG 5 cut(s) 6, 168, 771, 793, 1074
BstEII GGTNACC 1 cut(s) 620
BstF5I GGATG 2 cut(s) 47, 478
BstFNI CGCG 2 cut(s) 497, 1017
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 2 cut(s) 484, 497
BstKTI GATC 7 cut(s) 58, 108, 157, 231, 433, 561, 712
BstMAI GTCTC 2 cut(s) 264, 917
BstMBI GATC 7 cut(s) 55, 105, 154, 228, 430, 558, 709
BstMWI GCNNNNNNNGC 2 cut(s) 985, 1070
BstNSI RCATGY 1 cut(s) 306
BstPI GGTNACC 1 cut(s) 620
BstSCI CCNGG 2 cut(s) 205, 206
BstSFI CTRYAG 1 cut(s) 722
BstSLI GKGCMC 1 cut(s) 838
BstUI CGCG 2 cut(s) 497, 1017
BstV1I GCAGC 3 cut(s) 59, 1048, 1051
BstV2I GAAGAC 1 cut(s) 76
BstXI CCANNNNNNTGG 1 cut(s) 421
BsuI GTATCC 1 cut(s) 204
BsuRI GGCC 2 cut(s) 573, 653
BtsCI GGATG 2 cut(s) 47, 478
BtsI GCAGTG 2 cut(s) 603, 897
BtsIMutI CAGTG 6 cut(s) 150, 603, 630, 705, 768, 897
CaiI CAGNNNCTG 1 cut(s) 38
CfoI GCGC 2 cut(s) 484, 497
Cfr13I GGNCC 3 cut(s) 786, 841, 1012
Cfr9I CCCGGG 1 cut(s) 206
Csp6I GTAC 4 cut(s) 133, 439, 446, 1039
CviAII CATG 6 cut(s) 184, 190, 212, 303, 520, 626
CviQI GTAC 4 cut(s) 133, 439, 446, 1039
DdeI CTNAG 5 cut(s) 6, 168, 771, 793, 1074
DpnI GATC 7 cut(s) 57, 107, 156, 230, 432, 560, 711
DpnII GATC 7 cut(s) 55, 105, 154, 228, 430, 558, 709
DrdI GACNNNNNNGTC 1 cut(s) 698
DseDI GACNNNNNNGTC 1 cut(s) 698
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 3 cut(s) 88, 728, 878
EarI CTCTTC 3 cut(s) 88, 728, 878
Eco47I GGWCC 3 cut(s) 786, 841, 1012
Eco88I CYCGRG 1 cut(s) 206
Eco91I GGTNACC 1 cut(s) 620
EcoO65I GGTNACC 1 cut(s) 620
FaeI CATG 6 cut(s) 187, 193, 215, 306, 523, 629
FaqI GGGAC 2 cut(s) 398, 600
FatI CATG 6 cut(s) 183, 189, 211, 302, 519, 625
FauI CCCGC 2 cut(s) 490, 941
FbaI TGATCA 1 cut(s) 228
Fnu4HI GCNGC 5 cut(s) 48, 297, 485, 1062, 1065
FokI GGATG 2 cut(s) 54, 485
Fsp4HI GCNGC 5 cut(s) 48, 297, 485, 1062, 1065
GlaI GCGC 2 cut(s) 483, 496
GluI GCNGC 5 cut(s) 48, 297, 485, 1062, 1065
GsuI CTGGAG 1 cut(s) 341
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 2 cut(s) 573, 653
HapII CCGG 5 cut(s) 63, 207, 650, 839, 989
HhaI GCGC 2 cut(s) 484, 497
Hin1II CATG 6 cut(s) 187, 193, 215, 306, 523, 629
Hin6I GCGC 2 cut(s) 482, 495
HinP1I GCGC 2 cut(s) 482, 495
HinfI GANTC 3 cut(s) 13, 276, 460
HpaII CCGG 5 cut(s) 63, 207, 650, 839, 989
HphI GGTGA 4 cut(s) 388, 505, 514, 614
Hpy166II GTNNAC 4 cut(s) 363, 448, 513, 836
Hpy188I TCNGA 3 cut(s) 9, 578, 873
Hpy188III TCNNGA 3 cut(s) 39, 53, 232
Hpy8I GTNNAC 4 cut(s) 363, 448, 513, 836
Hpy99I CGWCG 1 cut(s) 692
HpyAV CCTTC 5 cut(s) 247, 260, 870, 889, 1098
HpyCH4III ACNGT 6 cut(s) 34, 137, 404, 508, 634, 763
HpyCH4IV ACGT 4 cut(s) 249, 441, 591, 965
HpyCH4V TGCA 5 cut(s) 324, 476, 724, 836, 1022
HpyF10VI GCNNNNNNNGC 2 cut(s) 985, 1070
HpyF3I CTNAG 5 cut(s) 6, 168, 771, 793, 1074
HpySE526I ACGT 4 cut(s) 249, 441, 591, 965
Hsp92II CATG 6 cut(s) 187, 193, 215, 306, 523, 629
HspAI GCGC 2 cut(s) 482, 495
KpnI GGTACC 1 cut(s) 1042
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 7 cut(s) 55, 105, 154, 228, 430, 558, 709
LmnI GCTCC 3 cut(s) 243, 360, 540
Lsp1109I GCAGC 3 cut(s) 59, 1048, 1051
LweI GCATC 3 cut(s) 384, 463, 1060
MaeII ACGT 4 cut(s) 249, 441, 591, 965
MaeIII GTNAC 4 cut(s) 115, 502, 620, 628
MalI GATC 7 cut(s) 57, 107, 156, 230, 432, 560, 711
MboI GATC 7 cut(s) 55, 105, 154, 228, 430, 558, 709
MboII GAAGA 7 cut(s) 76, 105, 370, 565, 568, 745, 895
MhlI GDGCHC 1 cut(s) 838
MluCI AATT 3 cut(s) 854, 915, 940
MlyI GAGTC 2 cut(s) 7, 285
MmeI TCCRAC 2 cut(s) 450, 990
MroXI GAANNNNTTC 2 cut(s) 257, 1087
MseI TTAA 1 cut(s) 755
MslI CAYNNNNRTG 3 cut(s) 188, 518, 681
MspI CCGG 5 cut(s) 63, 207, 650, 839, 989
MspR9I CCNGG 2 cut(s) 207, 208
Mva1269I GAATGC 1 cut(s) 1089
MvnI CGCG 2 cut(s) 497, 1017
MwoI GCNNNNNNNGC 2 cut(s) 985, 1070
NciI CCSGG 2 cut(s) 207, 208
NdeII GATC 7 cut(s) 55, 105, 154, 228, 430, 558, 709
NlaIII CATG 6 cut(s) 187, 193, 215, 306, 523, 629
NlaIV GGNNCC 3 cut(s) 685, 787, 1040
NmuCI GTSAC 4 cut(s) 115, 502, 620, 628
NspI RCATGY 1 cut(s) 306
PciI ACATGT 1 cut(s) 302
PctI GAATGC 1 cut(s) 1089
PdmI GAANNNNTTC 2 cut(s) 257, 1087
PfeI GAWTC 1 cut(s) 460
PflFI GACNNNGTC 1 cut(s) 275
PkrI GCNGC 5 cut(s) 49, 298, 486, 1063, 1066
PleI GAGTC 2 cut(s) 7, 284
PpsI GAGTC 2 cut(s) 7, 284
Ppu21I YACGTR 2 cut(s) 442, 592
PscI ACATGT 1 cut(s) 302
PspEI GGTNACC 1 cut(s) 620
PspN4I GGNNCC 3 cut(s) 685, 787, 1040
PspPI GGNCC 3 cut(s) 786, 841, 1012
PstI CTGCAG 1 cut(s) 726
PstNI CAGNNNCTG 1 cut(s) 38
PsyI GACNNNGTC 1 cut(s) 275
RsaI GTAC 4 cut(s) 134, 440, 447, 1040
RsaNI GTAC 4 cut(s) 133, 439, 446, 1039
RseI CAYNNNNRTG 3 cut(s) 188, 518, 681
SaqAI TTAA 1 cut(s) 755
SatI GCNGC 5 cut(s) 48, 297, 485, 1062, 1065
Sau3AI GATC 7 cut(s) 55, 105, 154, 228, 430, 558, 709
Sau96I GGNCC 3 cut(s) 786, 841, 1012
SchI GAGTC 2 cut(s) 7, 285
ScrFI CCNGG 2 cut(s) 207, 208
SduI GDGCHC 1 cut(s) 838
SfaNI GCATC 3 cut(s) 384, 463, 1060
SfcI CTRYAG 1 cut(s) 722
SinI GGWCC 3 cut(s) 786, 841, 1012
SmaI CCCGGG 1 cut(s) 208
SmiMI CAYNNNNRTG 3 cut(s) 188, 518, 681
Sse9I AATT 3 cut(s) 854, 915, 940
SsiI CCGC 6 cut(s) 296, 485, 497, 768, 948, 1015
StyD4I CCNGG 2 cut(s) 205, 206
TaaI ACNGT 6 cut(s) 34, 137, 404, 508, 634, 763
TaiI ACGT 4 cut(s) 252, 444, 594, 968
TaqI TCGA 2 cut(s) 744, 1081
TasI AATT 3 cut(s) 854, 915, 940
TatI WGTACW 1 cut(s) 132
TauI GCSGC 2 cut(s) 299, 487
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 1 cut(s) 755
Tru9I TTAA 1 cut(s) 755
TscAI CASTG 6 cut(s) 157, 603, 637, 712, 768, 897
TseFI GTSAC 4 cut(s) 115, 502, 620, 628
TseI GCWGC 3 cut(s) 47, 1061, 1064
Tsp45I GTSAC 4 cut(s) 115, 502, 620, 628
TspDTI ATGAA 4 cut(s) 536, 739, 852, 953
TspMI CCCGGG 1 cut(s) 206
TspRI CASTG 6 cut(s) 157, 603, 637, 712, 768, 897
Tth111I GACNNNGTC 1 cut(s) 275
VneI GTGCAC 1 cut(s) 834
VpaK11BI GGWCC 3 cut(s) 786, 841, 1012
XapI RAATTY 1 cut(s) 940
XceI RCATGY 1 cut(s) 306
XmaI CCCGGG 1 cut(s) 206
XmnI GAANNNNTTC 2 cut(s) 257, 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.