MD08G1009200.v1.1

cinnamyl alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012582
Physical Location & Seq
Reverse (-)
678733 .. 684320
5588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1009200.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGGCTCAGACGACTCCAAATCACACACAGACGGTTTCTGGGTGGGCAGCTTACGATTCCTCGGGCAAGATTACTCCTTTCGTCTTCAAACGAAGAGAAAATGGGATCAACGATGTGACCATAAAGATCTTGTATTGTGGGATATGCCACACCGATCTCCACCATGCTAAGAATGACTGGGGCATCACCATGTATCCAGTAGTTCCCGGGCATGAGATTACAGGACTGGTCACTAAAGTTGGGAGCAACGTGAAGGAGTTCAAGGAAGGAGACAGAGTCGGGGTTGGGTGTTTGGCGGCGACATGTCTGGAGTGTGAGTTCTGCAAGTCCTCCCAAGAGAACTACTGCGACCAGGTCCAGTTTACCTACAATGGCATCTTCTGGGACGGCAGCATCACCTACGGTGGCTACTCCAAAATGTTGGTCGCAGATCACAGGTACGTGGTACGCATACCAGACAATCTGCCGTTGGATGCAGCAGCGCCGCTATTGTGTGCGGGAGTGACGGTGTTCGCCCCCCTGAAAGACCACAACCTACACAAAACAACGGGGACGAAGATAGGGGTGGTTGGCCTCGGAGGTCTCGGGCACGTGGCGGTGAAGTTTGGGAAAGCTTTTGGTCACCATGTAACTGTCATCAGCACCTCTCCAGCCAAAGAGAAGGAGGCTAAAGAGCATTTGGGTGCCGACGACTTCCTCGTCAGCACTGATCCTCAACAATTGCAGAAAGGGAAGAGGACGCTCGACTTCATATTAAACACAGTGTCCGCTAAGCACTCGCTTGGGCCCATCTTAGAACTGCTCAAAGTGAATGGAACTATGGTGGTTGTGGGTGCACCGGACCAGCCTTTTGAGTTGCCTTCATTTCCTCTGATATTTGGCAAGAGAGCAGTGAAGGGAAGCGTAATAGGAGGAATAAGTGAGACAGCGGAGATGATGGATTTATGCGGGAAGCACAACATAACATGCGACATTGAGCTTACAACGCCGGACAAAATCAACGAGGCCTTGGACCGTGTCGCCAAGAATGATGTTAGGTATCGATTTGTCATTGACATTGCTGCTGGTGATGCTGCTAATGATCAAATACCTTCCAAACTTGCGAGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

371

Amino Acids

40.04

Weight (kDa)

6.53

Isoelectric Point (pI)

25.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 37 - 153 8.3e-27 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 194 - 316 3.2e-21 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 683
AciI CCGC 7 cut(s) 296, 485, 497, 596, 768, 929, 948
AclWI GGATC 2 cut(s) 113, 704
AcvI CACGTG 1 cut(s) 592
AfaI GTAC 2 cut(s) 440, 447
AflIII ACRYGT 1 cut(s) 302
AgsI TTSAA 2 cut(s) 88, 262
AjnI CCWGG 1 cut(s) 351
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
AluBI AGCT 3 cut(s) 50, 614, 979
AluI AGCT 3 cut(s) 50, 614, 979
Alw21I GWGCWC 1 cut(s) 838
Alw26I GTCTC 3 cut(s) 264, 587, 917
Alw44I GTGCAC 1 cut(s) 834
AlwI GGATC 2 cut(s) 113, 704
Ama87I CYCGRG 3 cut(s) 61, 206, 584
AoxI GGCC 3 cut(s) 571, 785, 1005
ApaI GGGCCC 1 cut(s) 789
ApaLI GTGCAC 1 cut(s) 834
ApeKI GCWGC 6 cut(s) 47, 390, 476, 479, 1061, 1073
ArsI GACNNNNNNTTYG 4 cut(s) 408, 440, 833, 865
Asp700I GAANNNNTTC 1 cut(s) 257
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 5 cut(s) 355, 785, 786, 841, 1012
AsuC2I CCSGG 2 cut(s) 207, 208
AsuHPI GGTGA 5 cut(s) 178, 388, 610, 614, 1079
AvaI CYCGRG 3 cut(s) 61, 206, 584
AvaII GGWCC 3 cut(s) 355, 841, 1012
BaeGI GKGCMC 3 cut(s) 591, 789, 838
BanI GGYRCC 1 cut(s) 683
BanII GRGCYC 1 cut(s) 789
BbrPI CACGTG 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 838
BbvI GCAGC 6 cut(s) 59, 402, 488, 491, 1048, 1060
BccI CCATC 2 cut(s) 797, 931
BceAI ACGGC 2 cut(s) 403, 451
BciT130I CCWGG 1 cut(s) 353
BciVI GTATCC 1 cut(s) 204
BclI TGATCA 1 cut(s) 1081
BcnI CCSGG 2 cut(s) 207, 208
BcoDI GTCTC 3 cut(s) 264, 587, 917
BfoI RGCGCY 1 cut(s) 485
BfuI GTATCC 1 cut(s) 204
BglII AGATCT 1 cut(s) 126
BisI GCNGC 8 cut(s) 48, 297, 391, 477, 480, 485, 1062, 1074
BlpI GCTNAGC 1 cut(s) 771
BlsI GCNGC 8 cut(s) 49, 298, 392, 478, 481, 486, 1063, 1075
Bme1390I CCNGG 3 cut(s) 207, 208, 353
Bme18I GGWCC 3 cut(s) 355, 841, 1012
BmeT110I CYCGRG 3 cut(s) 61, 206, 584
BmgT120I GGNCC 5 cut(s) 355, 785, 786, 841, 1012
BmiI GGNNCC 2 cut(s) 685, 787
BmrFI CCNGG 3 cut(s) 207, 208, 353
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 5 cut(s) 192, 384, 402, 463, 1060
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 76
BpmI CTGGAG 2 cut(s) 329, 633
Bpu1102I GCTNAGC 1 cut(s) 771
BpuMI CCSGG 2 cut(s) 207, 208
Bsa29I ATCGAT 1 cut(s) 1042
BsaAI YACGTR 2 cut(s) 442, 592
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 4 cut(s) 60, 206, 574, 1008
BsaWI WCCGGW 1 cut(s) 838
BsaXI ACNNNNNCTCC 6 cut(s) 261, 291, 302, 332, 492, 522
Bse1I ACTGG 4 cut(s) 182, 197, 231, 358
Bse3DI GCAATG 1 cut(s) 1056
BseBI CCWGG 1 cut(s) 353
BseCI ATCGAT 1 cut(s) 1042
BseDI CCNNGG 4 cut(s) 60, 206, 574, 1008
BseGI GGATG 1 cut(s) 478
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 4 cut(s) 182, 197, 231, 358
BseSI GKGCMC 3 cut(s) 591, 789, 838
BseXI GCAGC 6 cut(s) 59, 402, 488, 491, 1048, 1060
BshFI GGCC 3 cut(s) 573, 787, 1007
BshNI GGYRCC 1 cut(s) 683
BshVI ATCGAT 1 cut(s) 1042
BsiHKAI GWGCWC 1 cut(s) 838
BsiHKCI CYCGRG 3 cut(s) 61, 206, 584
BsiSI CCGG 3 cut(s) 207, 839, 989
BslFI GGGAC 2 cut(s) 398, 565
BsmAI GTCTC 3 cut(s) 264, 587, 917
BsmFI GGGAC 2 cut(s) 398, 565
BsnI GGCC 3 cut(s) 573, 787, 1007
Bso31I GGTCTC 1 cut(s) 587
BsoBI CYCGRG 3 cut(s) 61, 206, 584
Bsp120I GGGCCC 1 cut(s) 785
Bsp1286I GDGCHC 3 cut(s) 591, 789, 838
Bsp143I GATC 6 cut(s) 105, 126, 154, 430, 709, 1081
Bsp1720I GCTNAGC 1 cut(s) 771
BspACI CCGC 7 cut(s) 296, 485, 497, 596, 768, 929, 948
BspANI GGCC 3 cut(s) 573, 787, 1007
BspCNI CTCAG 1 cut(s) 19
BspDI ATCGAT 1 cut(s) 1042
BspLI GGNNCC 2 cut(s) 685, 787
BspPI GGATC 2 cut(s) 113, 704
BspT107I GGYRCC 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 1056
BsrI ACTGG 4 cut(s) 182, 197, 231, 358
BssECI CCNNGG 4 cut(s) 60, 206, 574, 1008
BssMI GATC 6 cut(s) 105, 126, 154, 430, 709, 1081
BssT1I CCWWGG 1 cut(s) 1008
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 6 cut(s) 34, 404, 508, 634, 763, 1016
Bst6I CTCTTC 2 cut(s) 88, 728
BstBAI YACGTR 2 cut(s) 442, 592
BstC8I GCNNGC 1 cut(s) 1105
BstDEI CTNAG 4 cut(s) 6, 168, 771, 793
BstEII GGTNACC 1 cut(s) 620
BstF5I GGATG 1 cut(s) 478
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 6 cut(s) 108, 129, 157, 433, 712, 1084
BstMAI GTCTC 3 cut(s) 264, 587, 917
BstMBI GATC 6 cut(s) 105, 126, 154, 430, 709, 1081
BstMWI GCNNNNNNNGC 2 cut(s) 985, 1070
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 2 cut(s) 306, 969
BstPI GGTNACC 1 cut(s) 620
BstSCI CCNGG 3 cut(s) 205, 206, 351
BstSLI GKGCMC 3 cut(s) 591, 789, 838
BstV1I GCAGC 6 cut(s) 59, 402, 488, 491, 1048, 1060
BstV2I GAAGAC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 421
BstYI RGATCY 1 cut(s) 126
Bsu15I ATCGAT 1 cut(s) 1042
BsuI GTATCC 1 cut(s) 204
BsuRI GGCC 3 cut(s) 573, 787, 1007
BsuTUI ATCGAT 1 cut(s) 1042
BtsCI GGATG 1 cut(s) 478
BtsI GCAGTG 1 cut(s) 897
BtsIMutI CAGTG 3 cut(s) 705, 768, 897
Cac8I GCNNGC 1 cut(s) 1105
CfoI GCGC 1 cut(s) 484
Cfr13I GGNCC 5 cut(s) 355, 785, 786, 841, 1012
Cfr9I CCCGGG 1 cut(s) 206
ClaI ATCGAT 1 cut(s) 1042
CseI GACGC 1 cut(s) 748
CsiI ACCWGGT 1 cut(s) 351
Csp6I GTAC 2 cut(s) 439, 446
CviAII CATG 6 cut(s) 164, 190, 212, 303, 626, 966
CviQI GTAC 2 cut(s) 439, 446
DdeI CTNAG 4 cut(s) 6, 168, 771, 793
DpnI GATC 6 cut(s) 107, 128, 156, 432, 711, 1083
DpnII GATC 6 cut(s) 105, 126, 154, 430, 709, 1081
DrdI GACNNNNNNGTC 1 cut(s) 698
DseDI GACNNNNNNGTC 1 cut(s) 698
Eam1104I CTCTTC 2 cut(s) 88, 728
EarI CTCTTC 2 cut(s) 88, 728
Eco130I CCWWGG 1 cut(s) 1008
Eco147I AGGCCT 1 cut(s) 1007
Eco24I GRGCYC 1 cut(s) 789
Eco31I GGTCTC 1 cut(s) 587
Eco47I GGWCC 3 cut(s) 355, 841, 1012
Eco72I CACGTG 1 cut(s) 592
Eco88I CYCGRG 3 cut(s) 61, 206, 584
Eco91I GGTNACC 1 cut(s) 620
EcoO65I GGTNACC 1 cut(s) 620
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 1008
EcoT38I GRGCYC 1 cut(s) 789
ErhI CCWWGG 1 cut(s) 1008
FaeI CATG 6 cut(s) 167, 193, 215, 306, 629, 969
FaqI GGGAC 2 cut(s) 398, 565
FatI CATG 6 cut(s) 163, 189, 211, 302, 625, 965
FauI CCCGC 2 cut(s) 490, 941
FbaI TGATCA 1 cut(s) 1081
Fnu4HI GCNGC 8 cut(s) 48, 297, 391, 477, 480, 485, 1062, 1074
FokI GGATG 1 cut(s) 485
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 8 cut(s) 48, 297, 391, 477, 480, 485, 1062, 1074
GlaI GCGC 1 cut(s) 483
GluI GCNGC 8 cut(s) 48, 297, 391, 477, 480, 485, 1062, 1074
GsuI CTGGAG 2 cut(s) 329, 633
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 3 cut(s) 573, 787, 1007
HapII CCGG 3 cut(s) 207, 839, 989
HgaI GACGC 1 cut(s) 748
HhaI GCGC 1 cut(s) 484
Hin1II CATG 6 cut(s) 167, 193, 215, 306, 629, 969
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HindIII AAGCTT 1 cut(s) 612
HinfI GANTC 3 cut(s) 13, 56, 276
HpaII CCGG 3 cut(s) 207, 839, 989
HphI GGTGA 5 cut(s) 178, 388, 610, 614, 1079
Hpy166II GTNNAC 2 cut(s) 363, 836
Hpy188I TCNGA 3 cut(s) 9, 578, 873
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 2 cut(s) 363, 836
Hpy99I CGWCG 1 cut(s) 692
HpyAV CCTTC 6 cut(s) 247, 260, 655, 870, 889, 1101
HpyCH4III ACNGT 6 cut(s) 34, 404, 508, 634, 763, 1016
HpyCH4IV ACGT 3 cut(s) 249, 441, 591
HpyCH4V TGCA 4 cut(s) 324, 476, 724, 836
HpyF10VI GCNNNNNNNGC 2 cut(s) 985, 1070
HpyF3I CTNAG 4 cut(s) 6, 168, 771, 793
HpySE526I ACGT 3 cut(s) 249, 441, 591
Hsp92II CATG 6 cut(s) 167, 193, 215, 306, 629, 969
HspAI GCGC 1 cut(s) 482
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 6 cut(s) 105, 126, 154, 430, 709, 1081
LmnI GCTCC 1 cut(s) 243
Lsp1109I GCAGC 6 cut(s) 59, 402, 488, 491, 1048, 1060
LweI GCATC 5 cut(s) 192, 384, 402, 463, 1060
MabI ACCWGGT 1 cut(s) 351
MaeII ACGT 3 cut(s) 249, 441, 591
MaeIII GTNAC 5 cut(s) 115, 229, 502, 620, 628
MalI GATC 6 cut(s) 107, 128, 156, 432, 711, 1083
MboI GATC 6 cut(s) 105, 126, 154, 430, 709, 1081
MboII GAAGA 5 cut(s) 76, 105, 370, 568, 745
MfeI CAATTG 1 cut(s) 719
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 3 cut(s) 591, 789, 838
MluCI AATT 1 cut(s) 719
MlyI GAGTC 2 cut(s) 7, 285
MmeI TCCRAC 1 cut(s) 450
MroXI GAANNNNTTC 1 cut(s) 257
MseI TTAA 1 cut(s) 755
MslI CAYNNNNRTG 2 cut(s) 188, 681
MspA1I CMGCKG 1 cut(s) 929
MspI CCGG 3 cut(s) 207, 839, 989
MspR9I CCNGG 3 cut(s) 207, 208, 353
MunI CAATTG 1 cut(s) 719
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 985, 1070
NciI CCSGG 2 cut(s) 207, 208
NdeII GATC 6 cut(s) 105, 126, 154, 430, 709, 1081
NlaIII CATG 6 cut(s) 167, 193, 215, 306, 629, 969
NlaIV GGNNCC 2 cut(s) 685, 787
NmuCI GTSAC 4 cut(s) 115, 229, 502, 620
NspI RCATGY 2 cut(s) 306, 969
PceI AGGCCT 1 cut(s) 1007
PciI ACATGT 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 56
PflFI GACNNNGTC 3 cut(s) 275, 353, 1016
PkrI GCNGC 8 cut(s) 49, 298, 392, 478, 481, 486, 1063, 1075
PleI GAGTC 2 cut(s) 7, 284
PmaCI CACGTG 1 cut(s) 592
PmlI CACGTG 1 cut(s) 592
PpsI GAGTC 2 cut(s) 7, 284
Ppu21I YACGTR 2 cut(s) 442, 592
PscI ACATGT 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 351
PspCI CACGTG 1 cut(s) 592
PspEI GGTNACC 1 cut(s) 620
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 685, 787
PspOMI GGGCCC 1 cut(s) 785
PspPI GGNCC 5 cut(s) 355, 785, 786, 841, 1012
PsuI RGATCY 1 cut(s) 126
PsyI GACNNNGTC 3 cut(s) 275, 353, 1016
RsaI GTAC 2 cut(s) 440, 447
RsaNI GTAC 2 cut(s) 439, 446
RseI CAYNNNNRTG 2 cut(s) 188, 681
SaqAI TTAA 1 cut(s) 755
SatI GCNGC 8 cut(s) 48, 297, 391, 477, 480, 485, 1062, 1074
Sau3AI GATC 6 cut(s) 105, 126, 154, 430, 709, 1081
Sau96I GGNCC 5 cut(s) 355, 785, 786, 841, 1012
SchI GAGTC 2 cut(s) 7, 285
ScrFI CCNGG 3 cut(s) 207, 208, 353
SduI GDGCHC 3 cut(s) 591, 789, 838
SexAI ACCWGGT 1 cut(s) 351
SfaNI GCATC 5 cut(s) 192, 384, 402, 463, 1060
SinI GGWCC 3 cut(s) 355, 841, 1012
SmaI CCCGGG 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 188, 681
Sse9I AATT 1 cut(s) 719
SseBI AGGCCT 1 cut(s) 1007
SsiI CCGC 7 cut(s) 296, 485, 497, 596, 768, 929, 948
StuI AGGCCT 1 cut(s) 1007
StyD4I CCNGG 3 cut(s) 205, 206, 351
StyI CCWWGG 1 cut(s) 1008
TaaI ACNGT 6 cut(s) 34, 404, 508, 634, 763, 1016
TaiI ACGT 3 cut(s) 252, 444, 594
TaqI TCGA 2 cut(s) 744, 1042
TasI AATT 1 cut(s) 719
TauI GCSGC 2 cut(s) 299, 487
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 1 cut(s) 755
Tru9I TTAA 1 cut(s) 755
TscAI CASTG 3 cut(s) 712, 768, 897
TseFI GTSAC 4 cut(s) 115, 229, 502, 620
TseI GCWGC 6 cut(s) 47, 390, 476, 479, 1061, 1073
Tsp45I GTSAC 4 cut(s) 115, 229, 502, 620
TspDTI ATGAA 2 cut(s) 739, 852
TspMI CCCGGG 1 cut(s) 206
TspRI CASTG 3 cut(s) 712, 768, 897
Tth111I GACNNNGTC 3 cut(s) 275, 353, 1016
VneI GTGCAC 1 cut(s) 834
VpaK11BI GGWCC 3 cut(s) 355, 841, 1012
XceI RCATGY 2 cut(s) 306, 969
XmaI CCCGGG 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.