pycom08g00740

cinnamyl alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012582
Physical Location & Seq
Reverse (-)
580931 .. 582107
1177 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00740.3

Sequence Viewer

Length: 711 bp
ATGGGTGAGCACATGTGGCTTCGGCTTCACATGTACGTGGTACACATACCAGACAACCTGCCATTGGATGCAGCAGCGCCGCTATTGTGTGCTGGAGTGACGGTGTTCGCCCCCCTGAAAGACCACAACCTACACAAAACAACGGGGAAGAAGATAGGGGTGGTTGGCCTCGGAGGTCTCGGGCACGTGGCGGTGAAGTTTGGGAAAGCTTTTGGTCACCATGTAACTGTCATCAGCACCTCTCCAGCCAAAGAGAAAGAGGCTAAAGAGCATTTGGGTGCCGACTACTTCCTAGTCAGCACTGATCCTCAACGATTGCAGAAAGGGAAGAGGACGCTCGACTTCATATTAAACACCGTGTCCGCTAAGCACTCGCTTGGGCCTATCTTAGAACTGCTCAAAGTGAACGGAACTATGGTGGTTGTGGGTGCACCGGACCAGCCTTTTGAGTTGCCTTCATTTCCTCTGATATTTGGCAAGAGAGCAGTGAAGGGAAGCGTAATAGGAGGAATAAGTGAGACAACGGAGATGATGAATTTATGCGGGAAGCACAACATAACGTGCGACATTGAGCTTACAACGCCGGACAAAATCAACGAGGCCTTGGACCGCGTCGCCAAGAATGATGTTAAGTATCGATTTGTCATTGACATTGCTGCTGGTGATGCTGATGCTAATGATCAAATATCTTCCAAACTTGCGGGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

237

Amino Acids

25.42

Weight (kDa)

7.79

Isoelectric Point (pI)

27.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 66
AccB1I GGYRCC 1 cut(s) 278
AccII CGCG 1 cut(s) 612
AciI CCGC 6 cut(s) 80, 191, 363, 543, 610, 701
AclWI GGATC 1 cut(s) 299
AcsI RAATTY 1 cut(s) 535
AcvI CACGTG 1 cut(s) 187
AfaI GTAC 2 cut(s) 35, 42
AfiI CCNNNNNNNGG 1 cut(s) 64
AflIII ACRYGT 2 cut(s) 12, 30
AluBI AGCT 2 cut(s) 209, 574
AluI AGCT 2 cut(s) 209, 574
Alw21I GWGCWC 2 cut(s) 12, 433
Alw26I GTCTC 2 cut(s) 182, 512
Alw44I GTGCAC 1 cut(s) 429
AlwI GGATC 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 4 cut(s) 166, 380, 600, 703
ApaLI GTGCAC 1 cut(s) 429
ApeKI GCWGC 3 cut(s) 71, 74, 656
ApoI RAATTY 1 cut(s) 535
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 4 cut(s) 380, 436, 607, 703
AsuHPI GGTGA 4 cut(s) 17, 205, 209, 674
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 2 cut(s) 436, 607
BaeGI GKGCMC 2 cut(s) 186, 433
BanI GGYRCC 1 cut(s) 278
BbrPI CACGTG 1 cut(s) 187
Bbv12I GWGCWC 2 cut(s) 12, 433
BbvI GCAGC 3 cut(s) 83, 86, 643
BclI TGATCA 1 cut(s) 679
BcoDI GTCTC 2 cut(s) 182, 512
BfaI CTAG 1 cut(s) 293
BfoI RGCGCY 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 66
BisI GCNGC 4 cut(s) 72, 75, 80, 657
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 4 cut(s) 73, 76, 81, 658
Bme18I GGWCC 2 cut(s) 436, 607
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 4 cut(s) 380, 436, 607, 703
BmiI GGNNCC 1 cut(s) 280
BmsI GCATC 3 cut(s) 58, 655, 661
BpmI CTGGAG 2 cut(s) 114, 228
Bpu1102I GCTNAGC 1 cut(s) 366
Bsa29I ATCGAT 1 cut(s) 637
BsaAI YACGTR 2 cut(s) 37, 187
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 2 cut(s) 169, 603
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 651
BseCI ATCGAT 1 cut(s) 637
BseDI CCNNGG 2 cut(s) 169, 603
BseGI GGATG 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 651
BseSI GKGCMC 2 cut(s) 186, 433
BseXI GCAGC 3 cut(s) 83, 86, 643
Bsh1236I CGCG 1 cut(s) 612
BshFI GGCC 4 cut(s) 168, 382, 602, 705
BshNI GGYRCC 1 cut(s) 278
BshVI ATCGAT 1 cut(s) 637
BsiHKAI GWGCWC 2 cut(s) 12, 433
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 2 cut(s) 434, 584
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 2 cut(s) 182, 512
BsnI GGCC 4 cut(s) 168, 382, 602, 705
Bso31I GGTCTC 1 cut(s) 182
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 12, 186, 433
Bsp143I GATC 2 cut(s) 304, 679
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 6 cut(s) 80, 191, 363, 543, 610, 701
BspANI GGCC 4 cut(s) 168, 382, 602, 705
BspDI ATCGAT 1 cut(s) 637
BspFNI CGCG 1 cut(s) 612
BspLI GGNNCC 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 66
BspPI GGATC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 278
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 651
BssECI CCNNGG 2 cut(s) 169, 603
BssMI GATC 2 cut(s) 304, 679
BssT1I CCWWGG 1 cut(s) 603
Bst4CI ACNGT 3 cut(s) 103, 229, 358
Bst6I CTCTTC 1 cut(s) 323
BstBAI YACGTR 2 cut(s) 37, 187
BstC8I GCNNGC 1 cut(s) 703
BstDEI CTNAG 2 cut(s) 366, 388
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 1 cut(s) 73
BstFNI CGCG 1 cut(s) 612
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 2 cut(s) 307, 682
BstMAI GTCTC 2 cut(s) 182, 512
BstMBI GATC 2 cut(s) 304, 679
BstMWI GCNNNNNNNGC 3 cut(s) 16, 580, 665
BstNSI RCATGY 2 cut(s) 16, 34
BstPI GGTNACC 1 cut(s) 215
BstSLI GKGCMC 2 cut(s) 186, 433
BstUI CGCG 1 cut(s) 612
BstV1I GCAGC 3 cut(s) 83, 86, 643
Bsu15I ATCGAT 1 cut(s) 637
BsuRI GGCC 4 cut(s) 168, 382, 602, 705
BsuTUI ATCGAT 1 cut(s) 637
BtsCI GGATG 1 cut(s) 73
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 2 cut(s) 300, 492
BveI ACCTGC 1 cut(s) 66
Cac8I GCNNGC 1 cut(s) 703
CfoI GCGC 1 cut(s) 79
Cfr13I GGNCC 4 cut(s) 380, 436, 607, 703
ClaI ATCGAT 1 cut(s) 637
CseI GACGC 2 cut(s) 343, 601
Csp6I GTAC 2 cut(s) 34, 41
CviAII CATG 3 cut(s) 13, 31, 221
CviQI GTAC 2 cut(s) 34, 41
DdeI CTNAG 2 cut(s) 366, 388
DpnI GATC 2 cut(s) 306, 681
DpnII GATC 2 cut(s) 304, 679
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
Eco130I CCWWGG 1 cut(s) 603
Eco147I AGGCCT 1 cut(s) 602
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 2 cut(s) 436, 607
Eco72I CACGTG 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 179
Eco91I GGTNACC 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 215
EcoT14I CCWWGG 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 3 cut(s) 16, 34, 224
FaiI YATR 8 cut(s) 14, 32, 47, 222, 347, 416, 541, 557
FatI CATG 3 cut(s) 12, 30, 220
FauI CCCGC 2 cut(s) 536, 694
FbaI TGATCA 1 cut(s) 679
Fnu4HI GCNGC 4 cut(s) 72, 75, 80, 657
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 4 cut(s) 72, 75, 80, 657
FspBI CTAG 1 cut(s) 293
GlaI GCGC 1 cut(s) 78
GluI GCNGC 4 cut(s) 72, 75, 80, 657
GsuI CTGGAG 2 cut(s) 114, 228
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 4 cut(s) 168, 382, 602, 705
HapII CCGG 2 cut(s) 434, 584
HgaI GACGC 2 cut(s) 343, 601
HhaI GCGC 1 cut(s) 79
Hin1II CATG 3 cut(s) 16, 34, 224
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HindIII AAGCTT 1 cut(s) 207
HpaII CCGG 2 cut(s) 434, 584
HphI GGTGA 4 cut(s) 17, 205, 209, 674
Hpy166II GTNNAC 3 cut(s) 43, 406, 431
Hpy188I TCNGA 2 cut(s) 173, 468
Hpy8I GTNNAC 3 cut(s) 43, 406, 431
Hpy99I CGWCG 1 cut(s) 617
HpyAV CCTTC 2 cut(s) 465, 484
HpyCH4III ACNGT 3 cut(s) 103, 229, 358
HpyCH4IV ACGT 3 cut(s) 36, 186, 560
HpyCH4V TGCA 3 cut(s) 71, 319, 431
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 580, 665
HpyF3I CTNAG 2 cut(s) 366, 388
HpySE526I ACGT 3 cut(s) 36, 186, 560
Hsp92II CATG 3 cut(s) 16, 34, 224
HspAI GCGC 1 cut(s) 77
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 2 cut(s) 304, 679
LpnPI CCDG 9 cut(s) 63, 71, 78, 128, 258, 447, 452, 597, 645
Lsp1109I GCAGC 3 cut(s) 83, 86, 643
LweI GCATC 3 cut(s) 58, 655, 661
MaeI CTAG 1 cut(s) 293
MaeII ACGT 3 cut(s) 36, 186, 560
MaeIII GTNAC 3 cut(s) 97, 215, 223
MalI GATC 2 cut(s) 306, 681
MboI GATC 2 cut(s) 304, 679
MboII GAAGA 4 cut(s) 160, 163, 340, 681
MhlI GDGCHC 3 cut(s) 12, 186, 433
MluCI AATT 1 cut(s) 535
MnlI CCTC 9 cut(s) 167, 179, 250, 253, 318, 324, 474, 500, 592
MseI TTAA 2 cut(s) 350, 630
MslI CAYNNNNRTG 2 cut(s) 35, 276
MspI CCGG 2 cut(s) 434, 584
MvnI CGCG 1 cut(s) 612
MwoI GCNNNNNNNGC 3 cut(s) 16, 580, 665
NdeII GATC 2 cut(s) 304, 679
NlaIII CATG 3 cut(s) 16, 34, 224
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 2 cut(s) 97, 215
NspI RCATGY 2 cut(s) 16, 34
PceI AGGCCT 1 cut(s) 602
PciI ACATGT 2 cut(s) 12, 30
PflFI GACNNNGTC 1 cut(s) 611
PkrI GCNGC 4 cut(s) 73, 76, 81, 658
PmaCI CACGTG 1 cut(s) 187
PmlI CACGTG 1 cut(s) 187
Ppu21I YACGTR 2 cut(s) 37, 187
PscI ACATGT 2 cut(s) 12, 30
PspCI CACGTG 1 cut(s) 187
PspEI GGTNACC 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 4 cut(s) 380, 436, 607, 703
PsyI GACNNNGTC 1 cut(s) 611
RsaI GTAC 2 cut(s) 35, 42
RsaNI GTAC 2 cut(s) 34, 41
RseI CAYNNNNRTG 2 cut(s) 35, 276
SaqAI TTAA 2 cut(s) 350, 630
SatI GCNGC 4 cut(s) 72, 75, 80, 657
Sau3AI GATC 2 cut(s) 304, 679
Sau96I GGNCC 4 cut(s) 380, 436, 607, 703
SduI GDGCHC 3 cut(s) 12, 186, 433
SetI ASST 9 cut(s) 39, 60, 132, 178, 189, 211, 242, 563, 576
SfaNI GCATC 3 cut(s) 58, 655, 661
SinI GGWCC 2 cut(s) 436, 607
SmiMI CAYNNNNRTG 2 cut(s) 35, 276
Sse9I AATT 1 cut(s) 535
SseBI AGGCCT 1 cut(s) 602
SsiI CCGC 6 cut(s) 80, 191, 363, 543, 610, 701
SspMI CTAG 1 cut(s) 293
StuI AGGCCT 1 cut(s) 602
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 3 cut(s) 103, 229, 358
TaiI ACGT 3 cut(s) 39, 189, 563
TaqI TCGA 2 cut(s) 339, 637
TasI AATT 1 cut(s) 535
TauI GCSGC 1 cut(s) 82
Tru1I TTAA 2 cut(s) 350, 630
Tru9I TTAA 2 cut(s) 350, 630
TscAI CASTG 2 cut(s) 307, 492
TseFI GTSAC 2 cut(s) 97, 215
TseI GCWGC 3 cut(s) 71, 74, 656
Tsp45I GTSAC 2 cut(s) 97, 215
TspDTI ATGAA 3 cut(s) 334, 447, 548
TspGWI ACGGA 2 cut(s) 423, 539
TspRI CASTG 2 cut(s) 307, 492
Tth111I GACNNNGTC 1 cut(s) 611
VneI GTGCAC 1 cut(s) 429
VpaK11BI GGWCC 2 cut(s) 436, 607
XapI RAATTY 1 cut(s) 535
XceI RCATGY 2 cut(s) 16, 34
XspI CTAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.