FvH4_2g39080

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011850
Physical Location & Seq
Forward (+)
28141997 .. 28145860
3864 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39080.t1

Sequence Viewer

Length: 3408 bp
ATGGCAACGGCTAGTTTCTTCTATGGTCTGATCATCCCAATGCTCTACTTCTCCGCCGCATTTACCGCCGCGCAACTCTCCGAGATCCAAGCCTTAACCTCCTTCAAGCTCAACCTCCACGACCCTCTCGGCGCCCTCACCGGCTGGGACTCCTCCACGCCCTCCGCCCCATGCGACTGGCGCGGCGTCGTCTGCTTCAACAACCGAGTCCGCGAGCTCACCCTCCCTCGCCTCCACCTCGCCGGCCGACTCTCCGACCAACTCGCCGCCCTCCGTGACCTCCGGAAGCTCAACCTCCACACCAACAACTTCAACGGCTCCATCCCTCCCTCTCTCTCCCAATGCGTGTTGCTACGCGCCGTCTACCTCCACGTCAACTCACTCTCCGGCGACCTCCCGCCGGCGATTCTCAACCTCACCAACCTCCAAATCCTCGCCCTTTCTCACAACTCCCTCTCCGGAAAAATCTCCGGCGAAATTCCGGCGAGCCTCCGGTACCTTGATCTCTCGTATAATGCATTCACCGGTGACATTCCGGTCAACTTCTCCGCCGACTCGCGGCTCCAGTTCATCAATTTCTCCTTCAACCAGTTCACCGGCGGCGTTCCGGCGACCATCGGCCGGCTCCAGACGCTTCAGTATCTCTGGCTGGACTCGAACAGGTTAACCGGTACGATCCCTTCTGCTATCTCCAACTGCTCCTCCCTCGTACATTTCAGCGCCGACGACAACGCGCTCCGAGGTCTAATTCCGGCCACTATCGGCGCCATTCCGAACCTTCAGGTGGTTTCTTTAGCTCGAAACAGCTTATCAGGCTCAGTCCCCACAGCTTTATTATGCAATGGGAATGATGACTCTTCTTCTTCTTCACTGAGGATAGTAGATCTAGGGTTCAATGCGCTCACGGGAATTGTGCCGCCGCAAAATGCAAGGTGTTCAAGTATCTTGGAGGTTCTGGTTCTTAAAAGCAATCGCATTCGCGGCGCGTTTCCGAGCTGGTTGAGCAATGTGACTAGGTTGAGGGTTTTCGATGTTTCTGGAAACTTGTTTTCGGGTGGATTGCCTCCTGAAATCGGCAATCTTTTCCGGTTGGAGGAGTTTCGGGTCGGTAATAATTCCCTCATTGGTGAAATTCCTGATCAGATTGCGAAATGCAGCTTGTTAAAGGCTCTTGATCTTGAAGGCAACCGCTTTAGGGGTCAGTTTCCGTTGTTTTTGGGAGGGATGAAGAGCTTGAAGGTGTTGTCTTTGGGTGGGAATTTGATCTCGGGTACCATCCCGTCGAGTTTGGGATCACTTTCGGGGCTGGAGTCGTTGAATCTGAGCGGTAACAATCTGACAGGGGAAGTTCCGGTTGAGGTCATGCAGCTAGGCAACTTGACTAGCTTGAACTTGAGTAATAACAAGTTTTCGGGTGAGGTTCTTGGGAATGTAGGAGCATTGAGGAGCTTGCAGGTTCTGAATGTGAGTAATTGTGGACTTTCGGGTAGAGTCCCTGCGAGTATTGGAGGTTTGATGAGGCTTGCTACGCTGGATTTGAGTAAACAAAAGCTTTCTGGTGAGTTGCCTACTGAGGTTTTTGGGCTGCCAAATTTACAAGCGGTTGCTTTGCAAGAGAATCAGTTATCAGGGGAGGTTCCTCAAGGTTTTAGTAGCTTGACTGGTTTAGTCTACCTAAATCTCTCCTCCAATGCCTTTACTGGTGTTATTCCATCCACTTATGGATATCTACGGTCACTGACTGTTCTATCTTTGTCAAAGAATCGCATTTCCGGTGTGATCCCACCAGAGCTTGGAGATAGTTTGAGCCTTCAAGTACTTGAGCTTAGTTCTAATCAATTGGAGGGCACGATTCCTGGTGATATCTCTCGGCTGTCTAGTTTGGAAGAGCTGGATGTAGGTCATAATAAACTGACAGGTGAAATACCAGAGGAAATTTCCAAATGCTCTGCTCTGACTTCATTATTGTTAGAGGGTAATCAACTTTCAGGTCCAATACCGAACTCCTTGCCTTTGTTATCGAACCTTGTGGTGTTGGATCTTTCCTACAACCAGTTGAGTGGAGCAATCCCGGCAAATATTTCACTCATTCCTCACTTGAAGTCATTAAATTTGTCTAACAACAACCTATCTGGTAAGATTCCAGAGCCATTGGGTTCTAAATTCAGTGGTTCTGCCTTCGCGATGAACCCGAGTTTATGTGGGAAACCATTGGACAGAGAATGTGGAGATGCAAAGAGGAGAAGGAGAAACAGGTTGATTTTATTGATTGCGGTGGCTGCTGGTGGAGCTTTGCTTCTGTTATTGTGTTGTTGTGGCTATGTTTACAGCCTCTACCGGTGGCGTAAGAAGCTTAGAGAAATGGTAACTGGGGAGAAGAAGCGCCCCAGTCCAGGTCGTGCAAGCTCAGGCGGAGAGAGATCTGGTCGCGGAAGTGGAGACAATGGAGGTCCAAAACTCGTTATGTTCAATAACAAGATTACATTTGCAGAGGCACTAGAAGCAACAAGACAATTTGATGAGGAAAATGTGTTGAGCAGGGGAAAGTATGGACTGGTGTTCAAGGCTTCTTTCCAAGATGGAATGGTGCTCTCAATTCGACGCCTTCATGCTTCTTCTCTTGGCGTGCTCGATGAAGAGGCTTTCCACAAAGAAGCCGAAGCTCTTGGCAAGGTGAAGCATCGAAATTTAACAGTTCTGCGAGGCTACTATGCAGGCCCTGATGTGAGGCTACTTGTATACGACTACATGCCTAATGGAAACTTGGCCACTCTGCTCCAAGAGGCTTCTCACCAAGATGGTCATGTACTAAATTGGCCAATGCGGCATCTCATTGCACTCGGCATTGCTAGAGGAATTGCTTTCCTGCACTCAGTCTCAATGGTTCATGGTGATGTGAAGCCGCAAAATGTTCTCTTCGACGCTGATTTTGAAGCCCACTTATCGGAATTTGGATTAGACCGGTTAACCATAATCTCACCAAAACCCTCATCCTCATCATCCACCATAGTTGGTACATTAGGCTATGTGTCACCCGAAGCAACACTAACCGGGCAAGCAACAAGAGAAGCCGATGTGTACAGTTTTGGAATTGTCCTGTTGGAGATACTAACCGGAAAGAAGCCTGTGATGTTCACCGAAGAAGAAGACATTGTGAAATGGGTGAAGAAGCAATTACAAAGAGGCCAAATCACCGAGCTGCTAGAGCCCGGACTACTTGAGCTAGACCCAGAGTCATCAGAATCAGAAGAGTTCTTACTAGGAATAAAAGTCGGACTCCTCTGCACCGCACCCGATCCACTTGACAGACCATCCATGGCTGACATTGTGTTCATGCTTGAAGGTTGCCGCGTCGGACCTGACATACCGTCGTCGGCCGATCCAACTTCATTGCCTTCACCGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1136

Amino Acids

121.88

Weight (kDa)

6.42

Isoelectric Point (pI)

36.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 65 5.5e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 386 - 489 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 388 - 446 2.6e-08 Leucine rich repeat
LRR_8 PF13855 411 - 470 2.7e-08 Leucine rich repeat
LRR_14 PF23598 482 - 713 2.6e-15 Leucine-rich repeat region
LRR_8 PF13855 507 - 565 6.2e-07 Leucine rich repeat
LRR_8 PF13855 530 - 590 2.9e-08 Leucine rich repeat
LRR_8 PF13855 555 - 614 1.2e-08 Leucine rich repeat
LRR_8 PF13855 603 - 662 5.7e-09 Leucine rich repeat
Pkinase PF00069 841 - 1109 2.3e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 842 - 1060 2.1e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75640
fragaria_vesca FvH4_2g39080
malus_domestica MD08G1017600.v1.1 MD08G1017800.v1.1 MD15G1016500.v1.1
prunus_persica Prupe.1G370300_v2.0.a1
pyrus_communis pycom08g01540 pycom15g01450 pycom15g01460
rosa_chinensis RchiOBHm_Chr6g0302191
rosa_laevigata RLG00000011188
rosa_multiflora Rmu_co8058424.1_g000001
rosa_roxburghii Rroxscaffold_7G00166010
rosa_rugosa Rorug06G0313000
rosa_samantha Rh6AG425500 Rh6BG477000 Rh6CG438300 Rh6DG425300
rosa_wichuraiana Rw6G036850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 536
Acc36I ACCTGC 1 cut(s) 1444
Acc65I GGTACC 2 cut(s) 495, 1271
AccB1I GGYRCC 4 cut(s) 131, 495, 764, 1271
AccB7I CCANNNNNTGG 1 cut(s) 1793
AccBSI CCGCTC 1 cut(s) 1326
AccI GTMKAC 3 cut(s) 363, 1671, 2739
AccIII TCCGGA 2 cut(s) 282, 458
AclWI GGATC 7 cut(s) 79, 670, 1300, 1774, 2046, 3290, 3374
AcoI YGGCCR 6 cut(s) 244, 619, 753, 2766, 2816, 3375
AcsI RAATTY 9 cut(s) 477, 1131, 1258, 1591, 1935, 2110, 2162, 2686, 2948
AcuI CTGAAG 2 cut(s) 620, 764
AcyI GRCGYC 4 cut(s) 132, 186, 765, 2602
AfaI GTAC 8 cut(s) 497, 673, 711, 1273, 1818, 2808, 3016, 3080
AgeI ACCGGT 4 cut(s) 524, 668, 2337, 2961
AhdI GACNNNNNGTC 2 cut(s) 3232, 3367
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 2 cut(s) 1855, 2392
Alw21I GWGCWC 3 cut(s) 219, 2592, 2631
Alw26I GTCTC 2 cut(s) 2433, 2881
AlwI GGATC 7 cut(s) 79, 670, 1300, 1774, 2046, 3290, 3374
AlwNI CAGNNNCTG 2 cut(s) 1459, 2720
Ama87I CYCGRG 2 cut(s) 1267, 2191
Aor13HI TCCGGA 2 cut(s) 282, 458
AoxI GGCC 9 cut(s) 244, 619, 753, 2716, 2766, 2816, 3184, 3375, 3402
ApeKI GCWGC 5 cut(s) 1155, 1366, 1585, 2279, 3199
ApoI RAATTY 9 cut(s) 477, 1131, 1258, 1591, 1935, 2110, 2162, 2686, 2948
ArsI GACNNNNNNTTYG 2 cut(s) 2912, 2944
AsiGI ACCGGT 4 cut(s) 524, 668, 2337, 2961
Asp718I GGTACC 2 cut(s) 495, 1271
AspS9I GGNCC 4 cut(s) 1991, 2450, 2717, 3356
AsuC2I CCSGG 3 cut(s) 2072, 3052, 3210
AvaI CYCGRG 2 cut(s) 1267, 2191
AvaII GGWCC 3 cut(s) 1991, 2450, 3356
BaeGI GKGCMC 1 cut(s) 1850
BalI TGGCCA 2 cut(s) 2768, 2818
BanI GGYRCC 4 cut(s) 131, 495, 764, 1271
BanII GRGCYC 2 cut(s) 219, 3210
BarI GAAGNNNNNNTAC 4 cut(s) 664, 696, 3240, 3272
BbsI GAAGAC 1 cut(s) 3153
Bbv12I GWGCWC 3 cut(s) 219, 2592, 2631
BbvI GCAGC 5 cut(s) 1167, 1378, 1572, 2266, 3186
BccI CCATC 7 cut(s) 329, 623, 1283, 1720, 2573, 2792, 3319
BceAI ACGGC 3 cut(s) 24, 331, 344
BcgI CGANNNNNNTGC 2 cut(s) 1990, 2024
BciT130I CCWGG 2 cut(s) 1857, 2394
BclI TGATCA 2 cut(s) 30, 1138
BcnI CCSGG 3 cut(s) 2072, 3052, 3210
BcoDI GTCTC 2 cut(s) 2433, 2881
BfoI RGCGCY 4 cut(s) 135, 723, 768, 2386
BfuAI ACCTGC 1 cut(s) 1444
BglI GCCNNNNNGGC 1 cut(s) 2824
BglII AGATCT 2 cut(s) 883, 2420
BmcAI AGTACT 1 cut(s) 1818
Bme1390I CCNGG 5 cut(s) 1857, 2072, 2394, 3052, 3210
Bme18I GGWCC 3 cut(s) 1991, 2450, 3356
BmeRI GACNNNNNGTC 2 cut(s) 3232, 3367
BmeT110I CYCGRG 2 cut(s) 1267, 2191
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 4 cut(s) 1991, 2450, 2717, 3356
BmiI GGNNCC 8 cut(s) 133, 319, 497, 563, 626, 766, 1273, 1638
BmrFI CCNGG 5 cut(s) 1857, 2072, 2394, 3052, 3210
BmrI ACTGGG 2 cut(s) 2379, 2382
BmsI GCATC 3 cut(s) 2221, 2689, 2836
BmuI ACTGGG 2 cut(s) 2379, 2382
BpiI GAAGAC 1 cut(s) 3153
BpmI CTGGAG 3 cut(s) 548, 611, 1328
Bpu10I CCTNAGC 1 cut(s) 2407
BpuEI CTTGAG 4 cut(s) 1414, 1626, 1841, 3239
BpuMI CCSGG 3 cut(s) 2072, 3052, 3210
BsaHI GRCGYC 4 cut(s) 132, 186, 765, 2602
BsaJI CCNNGG 2 cut(s) 739, 3315
BsaXI ACNNNNNCTCC 6 cut(s) 368, 398, 440, 470, 686, 716
Bse1I ACTGG 9 cut(s) 182, 565, 589, 1666, 1705, 2053, 2374, 2388, 2559
Bse3DI GCAATG 5 cut(s) 847, 1012, 2832, 2844, 3389
BseAI TCCGGA 2 cut(s) 282, 458
BseBI CCWGG 2 cut(s) 1857, 2394
BseDI CCNNGG 2 cut(s) 739, 3315
BseGI GGATG 9 cut(s) 33, 321, 1230, 1275, 1712, 1900, 2990, 2999, 3311
BseMI GCAATG 5 cut(s) 847, 1012, 2832, 2844, 3389
BseMII CTCAG 6 cut(s) 831, 863, 1313, 1563, 2421, 2886
BseNI ACTGG 9 cut(s) 182, 565, 589, 1666, 1705, 2053, 2374, 2388, 2559
BseRI GAGGAG 7 cut(s) 142, 691, 1109, 1459, 1675, 2254, 3269
BseSI GKGCMC 1 cut(s) 1850
BseX3I CGGCCG 3 cut(s) 244, 619, 3375
BseXI GCAGC 5 cut(s) 1167, 1378, 1572, 2266, 3186
BseYI CCCAGC 1 cut(s) 144
BsgI GTGCAG 2 cut(s) 2852, 3268
Bsh1285I CGRYCG 3 cut(s) 247, 622, 3378
BshFI GGCC 9 cut(s) 246, 621, 755, 2718, 2768, 2818, 3186, 3377, 3404
BshNI GGYRCC 4 cut(s) 131, 495, 764, 1271
BshTI ACCGGT 4 cut(s) 524, 668, 2337, 2961
BsiEI CGRYCG 3 cut(s) 247, 622, 3378
BsiHKAI GWGCWC 3 cut(s) 219, 2592, 2631
BsiHKCI CYCGRG 2 cut(s) 1267, 2191
BslFI GGGAC 3 cut(s) 161, 806, 1478
BsmAI GTCTC 2 cut(s) 2433, 2881
BsmFI GGGAC 3 cut(s) 161, 806, 1478
BsmI GAATGC 2 cut(s) 518, 975
BsnI GGCC 9 cut(s) 246, 621, 755, 2718, 2768, 2818, 3186, 3377, 3404
BsoBI CYCGRG 2 cut(s) 1267, 2191
Bsp1286I GDGCHC 5 cut(s) 219, 1850, 2592, 2631, 3210
Bsp13I TCCGGA 2 cut(s) 282, 458
Bsp1407I TGTACA 1 cut(s) 3078
Bsp19I CCATGG 1 cut(s) 3315
Bsp68I TCGCGA 1 cut(s) 2183
BspANI GGCC 9 cut(s) 246, 621, 755, 2718, 2768, 2818, 3186, 3377, 3404
BspCNI CTCAG 6 cut(s) 830, 864, 1314, 1564, 2420, 2885
BspEI TCCGGA 2 cut(s) 282, 458
BspLI GGNNCC 8 cut(s) 133, 319, 497, 563, 626, 766, 1273, 1638
BspMI ACCTGC 1 cut(s) 1444
BspPI GGATC 7 cut(s) 79, 670, 1300, 1774, 2046, 3290, 3374
BspQI GCTCTTC 2 cut(s) 1223, 1881
BspT107I GGYRCC 4 cut(s) 131, 495, 764, 1271
BsrBI CCGCTC 1 cut(s) 1326
BsrDI GCAATG 5 cut(s) 847, 1012, 2832, 2844, 3389
BsrGI TGTACA 1 cut(s) 3078
BsrI ACTGG 9 cut(s) 182, 565, 589, 1666, 1705, 2053, 2374, 2388, 2559
BssECI CCNNGG 2 cut(s) 739, 3315
BssNAI GTATAC 1 cut(s) 2740
BssNI GRCGYC 4 cut(s) 132, 186, 765, 2602
BssT1I CCWWGG 1 cut(s) 3315
Bst1107I GTATAC 1 cut(s) 2740
Bst2UI CCWGG 2 cut(s) 1857, 2394
Bst4CI ACNGT 5 cut(s) 1734, 1744, 2695, 3083, 3369
Bst6I CTCTTC 6 cut(s) 862, 1223, 1881, 2631, 2921, 3243
BstACI GRCGYC 4 cut(s) 132, 186, 765, 2602
BstAUI TGTACA 1 cut(s) 3078
BstDEI CTNAG 8 cut(s) 817, 872, 1322, 1572, 1826, 2354, 2407, 2872
BstDSI CCRYGG 1 cut(s) 3315
BstF5I GGATG 9 cut(s) 33, 321, 1230, 1275, 1712, 1900, 2990, 2999, 3311
BstH2I RGCGCY 4 cut(s) 135, 723, 768, 2386
BstMAI GTCTC 2 cut(s) 2433, 2881
BstMCI CGRYCG 3 cut(s) 247, 622, 3378
BstNI CCWGG 2 cut(s) 1857, 2394
BstNSI RCATGY 1 cut(s) 2752
BstSCI CCNGG 5 cut(s) 1855, 2070, 2392, 3050, 3208
BstSLI GKGCMC 1 cut(s) 1850
BstV1I GCAGC 5 cut(s) 1167, 1378, 1572, 2266, 3186
BstV2I GAAGAC 1 cut(s) 3153
BstX2I RGATCY 4 cut(s) 84, 883, 2038, 2420
BstXI CCANNNNNNTGG 2 cut(s) 177, 2060
BstYI RGATCY 4 cut(s) 84, 883, 2038, 2420
BstZ17I GTATAC 1 cut(s) 2740
BstZI CGGCCG 3 cut(s) 244, 619, 3375
BsuRI GGCC 9 cut(s) 246, 621, 755, 2718, 2768, 2818, 3186, 3377, 3404
BtgI CCRYGG 1 cut(s) 3315
BtgZI GCGATG 1 cut(s) 2198
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 9 cut(s) 33, 321, 1230, 1275, 1712, 1900, 2990, 2999, 3311
BtsIMutI CAGTG 3 cut(s) 869, 1736, 2173
BtuMI TCGCGA 1 cut(s) 2183
BveI ACCTGC 1 cut(s) 1444
CaiI CAGNNNCTG 2 cut(s) 1459, 2720
Cfr13I GGNCC 4 cut(s) 1991, 2450, 2717, 3356
CseI GACGC 5 cut(s) 175, 640, 2610, 2930, 3340
Csp6I GTAC 8 cut(s) 496, 672, 710, 1272, 1817, 2807, 3015, 3079
CspAI ACCGGT 4 cut(s) 524, 668, 2337, 2961
CspCI CAANNNNNGTGG 2 cut(s) 2992, 3027
CviAII CATG 8 cut(s) 171, 1363, 2609, 2749, 2804, 2888, 3316, 3334
CviQI GTAC 8 cut(s) 496, 672, 710, 1272, 1817, 2807, 3015, 3079
DdeI CTNAG 8 cut(s) 817, 872, 1322, 1572, 1826, 2354, 2407, 2872
DinI GGCGCC 2 cut(s) 133, 766
DrdI GACNNNNNNGTC 1 cut(s) 536
DriI GACNNNNNGTC 2 cut(s) 3232, 3367
DseDI GACNNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 6 cut(s) 244, 619, 753, 2766, 2816, 3375
EagI CGGCCG 3 cut(s) 244, 619, 3375
Eam1104I CTCTTC 6 cut(s) 862, 1223, 1881, 2631, 2921, 3243
Eam1105I GACNNNNNGTC 2 cut(s) 3232, 3367
EarI CTCTTC 6 cut(s) 862, 1223, 1881, 2631, 2921, 3243
EciI GGCGGA 4 cut(s) 43, 154, 538, 2427
Ecl136II GAGCTC 1 cut(s) 217
EclXI CGGCCG 3 cut(s) 244, 619, 3375
Eco130I CCWWGG 1 cut(s) 3315
Eco24I GRGCYC 2 cut(s) 219, 3210
Eco32I GATATC 2 cut(s) 1727, 1864
Eco47I GGWCC 3 cut(s) 1991, 2450, 3356
Eco52I CGGCCG 3 cut(s) 244, 619, 3375
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 620, 764
Eco88I CYCGRG 2 cut(s) 1267, 2191
EcoICRI GAGCTC 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 2717
EcoRII CCWGG 2 cut(s) 1855, 2392
EcoRV GATATC 2 cut(s) 1727, 1864
EcoT14I CCWWGG 1 cut(s) 3315
EcoT22I ATGCAT 1 cut(s) 520
EcoT38I GRGCYC 2 cut(s) 219, 3210
EgeI GGCGCC 2 cut(s) 133, 766
EheI GGCGCC 2 cut(s) 133, 766
ErhI CCWWGG 1 cut(s) 3315
FaeI CATG 8 cut(s) 174, 1366, 2612, 2752, 2807, 2891, 3319, 3337
FaqI GGGAC 3 cut(s) 161, 806, 1478
FatI CATG 8 cut(s) 170, 1362, 2608, 2748, 2803, 2887, 3315, 3333
FauI CCCGC 1 cut(s) 405
FbaI TGATCA 2 cut(s) 30, 1138
FblI GTMKAC 3 cut(s) 363, 1671, 2739
FokI GGATG 9 cut(s) 20, 308, 1237, 1262, 1699, 1907, 2977, 2986, 3298
FriOI GRGCYC 2 cut(s) 219, 3210
GsaI CCCAGC 1 cut(s) 148
GsuI CTGGAG 3 cut(s) 548, 611, 1328
HaeII RGCGCY 4 cut(s) 135, 723, 768, 2386
HaeIII GGCC 9 cut(s) 246, 621, 755, 2718, 2768, 2818, 3186, 3377, 3404
HgaI GACGC 5 cut(s) 175, 640, 2610, 2930, 3340
Hin1I GRCGYC 4 cut(s) 132, 186, 765, 2602
Hin1II CATG 8 cut(s) 174, 1366, 2612, 2752, 2807, 2891, 3319, 3337
HincII GTYRAC 4 cut(s) 376, 541, 666, 2967
HindII GTYRAC 4 cut(s) 376, 541, 666, 2967
HindIII AAGCTT 2 cut(s) 1550, 2351
HpaI GTTAAC 2 cut(s) 666, 2967
Hpy99I CGWCG 8 cut(s) 191, 728, 1285, 2604, 2924, 3356, 3373, 3376
HpyCH4III ACNGT 5 cut(s) 1734, 1744, 2695, 3083, 3369
HpyCH4IV ACGT 1 cut(s) 372
HpyF3I CTNAG 8 cut(s) 817, 872, 1322, 1572, 1826, 2354, 2407, 2872
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 4 cut(s) 132, 186, 765, 2602
Hsp92II CATG 8 cut(s) 174, 1366, 2612, 2752, 2807, 2891, 3319, 3337
KasI GGCGCC 2 cut(s) 131, 764
Kpn2I TCCGGA 2 cut(s) 282, 458
KpnI GGTACC 2 cut(s) 499, 1275
KroI GCCGGC 3 cut(s) 242, 400, 621
KroNI GCCGGC 3 cut(s) 244, 402, 623
Ksp22I TGATCA 2 cut(s) 30, 1138
KspAI GTTAAC 2 cut(s) 666, 2967
LguI GCTCTTC 2 cut(s) 1223, 1881
Lsp1109I GCAGC 5 cut(s) 1167, 1378, 1572, 2266, 3186
LweI GCATC 3 cut(s) 2221, 2689, 2836
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 7 cut(s) 275, 527, 1009, 1328, 1734, 2365, 3030
MbiI CCGCTC 1 cut(s) 1326
MfeI CAATTG 1 cut(s) 1838
MflI RGATCY 4 cut(s) 84, 883, 2038, 2420
MhlI GDGCHC 5 cut(s) 219, 1850, 2592, 2631, 3210
MlsI TGGCCA 2 cut(s) 2768, 2818
MluNI TGGCCA 2 cut(s) 2768, 2818
Mly113I GGCGCC 2 cut(s) 132, 765
MmeI TCCRAC 8 cut(s) 279, 717, 1071, 2016, 3081, 3253, 3334, 3407
Mox20I TGGCCA 2 cut(s) 2768, 2818
Mph1103I ATGCAT 1 cut(s) 520
MreI CGCCGGCG 1 cut(s) 400
MroI TCCGGA 2 cut(s) 282, 458
MroNI GCCGGC 3 cut(s) 242, 400, 621
MscI TGGCCA 2 cut(s) 2768, 2818
MseI TTAA 7 cut(s) 95, 665, 963, 1163, 2108, 2690, 2966
MslI CAYNNNNRTG 3 cut(s) 38, 2796, 2892
Msp20I TGGCCA 2 cut(s) 2768, 2818
MspR9I CCNGG 5 cut(s) 1857, 2072, 2394, 3052, 3210
MunI CAATTG 1 cut(s) 1838
Mva1269I GAATGC 2 cut(s) 518, 975
MvaI CCWGG 2 cut(s) 1857, 2394
NaeI GCCGGC 3 cut(s) 244, 402, 623
NarI GGCGCC 2 cut(s) 132, 765
NciI CCSGG 3 cut(s) 2072, 3052, 3210
NcoI CCATGG 1 cut(s) 3315
NgoMIV GCCGGC 3 cut(s) 242, 400, 621
NlaIII CATG 8 cut(s) 174, 1366, 2612, 2752, 2807, 2891, 3319, 3337
NlaIV GGNNCC 8 cut(s) 133, 319, 497, 563, 626, 766, 1273, 1638
NmeAIII GCCGAG 3 cut(s) 108, 1849, 2820
NmuCI GTSAC 5 cut(s) 275, 527, 1009, 1734, 3030
NruI TCGCGA 1 cut(s) 2183
NsiI ATGCAT 1 cut(s) 520
NspI RCATGY 1 cut(s) 2752
PciSI GCTCTTC 2 cut(s) 1223, 1881
PctI GAATGC 2 cut(s) 518, 975
PdiI GCCGGC 3 cut(s) 244, 402, 623
PfeI GAWTC 7 cut(s) 406, 1318, 1618, 1762, 1852, 2140, 3242
PflMI CCANNNNNTGG 1 cut(s) 1793
PinAI ACCGGT 4 cut(s) 524, 668, 2337, 2961
PluTI GGCGCC 2 cut(s) 135, 768
Psp124BI GAGCTC 1 cut(s) 219
Psp6I CCWGG 2 cut(s) 1855, 2392
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 2 cut(s) 1855, 2392
PspN4I GGNNCC 8 cut(s) 133, 319, 497, 563, 626, 766, 1273, 1638
PspPI GGNCC 4 cut(s) 1991, 2450, 2717, 3356
PstNI CAGNNNCTG 2 cut(s) 1459, 2720
PsuI RGATCY 4 cut(s) 84, 883, 2038, 2420
RruI TCGCGA 1 cut(s) 2183
RsaI GTAC 8 cut(s) 497, 673, 711, 1273, 1818, 2808, 3016, 3080
RsaNI GTAC 8 cut(s) 496, 672, 710, 1272, 1817, 2807, 3015, 3079
RseI CAYNNNNRTG 3 cut(s) 38, 2796, 2892
SacI GAGCTC 1 cut(s) 219
SapI GCTCTTC 2 cut(s) 1223, 1881
SaqAI TTAA 7 cut(s) 95, 665, 963, 1163, 2108, 2690, 2966
Sau96I GGNCC 4 cut(s) 1991, 2450, 2717, 3356
ScaI AGTACT 1 cut(s) 1818
ScrFI CCNGG 5 cut(s) 1857, 2072, 2394, 3052, 3210
SduI GDGCHC 5 cut(s) 219, 1850, 2592, 2631, 3210
SfaNI GCATC 3 cut(s) 2221, 2689, 2836
SfoI GGCGCC 2 cut(s) 133, 766
SgrAI CRCCGGYG 3 cut(s) 400, 524, 596
SinI GGWCC 3 cut(s) 1991, 2450, 3356
SmiMI CAYNNNNRTG 3 cut(s) 38, 2796, 2892
SmlI CTYRAG 4 cut(s) 1393, 1641, 1820, 3218
SmoI CTYRAG 4 cut(s) 1393, 1641, 1820, 3218
SspDI GGCGCC 2 cut(s) 131, 764
SspI AATATT 1 cut(s) 2080
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 5 cut(s) 1855, 2070, 2392, 3050, 3208
StyI CCWWGG 1 cut(s) 3315
TaaI ACNGT 5 cut(s) 1734, 1744, 2695, 3083, 3369
TaiI ACGT 1 cut(s) 375
TaqI TCGA 9 cut(s) 656, 799, 1029, 1283, 2021, 2599, 2631, 2683, 2919
TatI WGTACW 3 cut(s) 1816, 2806, 3078
TfiI GAWTC 7 cut(s) 406, 1318, 1618, 1762, 1852, 2140, 3242
Tru1I TTAA 7 cut(s) 95, 665, 963, 1163, 2108, 2690, 2966
Tru9I TTAA 7 cut(s) 95, 665, 963, 1163, 2108, 2690, 2966
TscAI CASTG 3 cut(s) 876, 1743, 2173
TseFI GTSAC 5 cut(s) 275, 527, 1009, 1734, 3030
TseI GCWGC 5 cut(s) 1155, 1366, 1585, 2279, 3199
Tsp45I GTSAC 5 cut(s) 275, 527, 1009, 1734, 3030
TspDTI ATGAA 9 cut(s) 559, 1241, 1950, 2201, 2597, 2649, 2876, 3322, 3378
TspGWI ACGGA 2 cut(s) 263, 1197
TspRI CASTG 3 cut(s) 876, 1743, 2173
Van91I CCANNNNNTGG 1 cut(s) 1793
VpaK11BI GGWCC 3 cut(s) 1991, 2450, 3356
XapI RAATTY 9 cut(s) 477, 1131, 1258, 1591, 1935, 2110, 2162, 2686, 2948
XceI RCATGY 1 cut(s) 2752
XmiI GTMKAC 3 cut(s) 363, 1671, 2739
ZrmI AGTACT 1 cut(s) 1818
Zsp2I ATGCAT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.