Rorug06G0313000

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011850
Physical Location & Seq
Reverse (-)
46444519 .. 46446328
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0313000.1

Sequence Viewer

Length: 1503 bp
ATGTTTCTCGAGCTACTAGCCTTAGCCCTTTTCTTGCTCCCCTTGCTTTTCCTCATTCTCAAGTTTTCCGGCAACCCGGAAACCGGCAACAGCAAGCTCCCAAAATCATACCCAATAATCGGTTCATTCCCAGCCCTCTACAAGAACCATGCCAACAGACTCGCATGGTTCACTGAGATTCTCCAGAACTCTCCCTCGGCCACTATCTCTATTCACCACTCTTTCGGCCAACACTCCGTCATCACCGCCAACCCCGCTGTGGTTCAGCACATCCTCAAGACAAACTTCCCCACCTACCAAAAAGGCGAGTCATCCCGTCAAATTCTCCAGGAGCTCCTCGGCGACGGCATTTTCAACGCCGACGGTGACAACTGGAAGTTCCAGAGGCAACTCTCCAGCCACGAATTCAACACCAAGTCGCTGAGAAAATTCGTTGAAGACGTGGTTGACATTGAACTCTACGACCGGCTCATACCCATTCTATCCAAATCTTCTAGCGAGCGAACCATTCTTGATTTCCAAGACATTCTCCAAAGGTTTGCCTTCGACAACATTTGCCGAATCGGGTTCGGCTACGACCCTGAGTATCTCCTTCCTTCTCTGCCGCAAGCTAAATTCGCGGTGGCATTTGACGATGCGGTGCAGATCAGCAGCGAGAGGTTCGGCTTCCTGAGACCGGTGTGGAAAATCAAAAGAGCTCTTGGAATCGGCTCGGAAAGCCGTATCAGCAGAGTGGTCTCCGATGTGCGTGAGTTCGCCAACACCATTGTCAAGCAAAAGAAGAGAGAGATGGACGAGATGAAGACCCAGGAGTCTCACGATCTGCTGTCTCGGTTCTTGAGCTCCGGCCACTCCGACGAGAAGTTCGTCACCGACATCGTCATCAGCTTCACCCTCGCCGGAAGAGACACGACCTCAGCGGCTCTGACCTGGTTCTTCTGGCTAATCTCAAACCACCCAAACGTCGAGAGCGAAATTCTCAAGGAGATAGAAGAGAAATCGGAGAACGGAGCCGGATATGATGAAGCGAAGGAGATGGTGTACACACACGCGGCTCTCTGCGAGAGCATGAGGCTGTACCCGCCGGTTCCGATCGACAGCAAAGAGGCGGTGAACGACGACGTTTTGCCGGACGGGACTAAAGTGAAGAAGGGGATGGCAGTGTCGTACCATGTTTACGCGATGGGGAGGATGGAGCAGATATGGGGCAAGGACTGGGCAGAGTTCCGACCAGAGAGATGGTTGGAGCGTGACGTGGCGGCGGACAAGTGGAGGTTCGTGGCGAAGGACTCGTTTAGCTACCCGGTGTTTCAGGCGGGGCCGAGGATCTGTTTGGGGAAGGAGATGGCGTTCTTGCAGATGAAGAGGGTGGTTGTTGGGGTTTTGAAGAGGTTTAAGGTGGTGCCGAAGAAGAGAGAGAATGGATTAGAGCCGGAGTTTGTTGCTTACCTTACCGCAAAAATGAAGGGTGGGTTTCCGGTCACCGTTGTGGAGAGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

500

Amino Acids

57.01

Weight (kDa)

7.71

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 472 8.3e-61 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75640
fragaria_vesca FvH4_2g39080
malus_domestica MD08G1017600.v1.1 MD08G1017800.v1.1 MD15G1016500.v1.1
prunus_persica Prupe.1G370300_v2.0.a1
pyrus_communis pycom08g01540 pycom15g01450 pycom15g01460
rosa_chinensis RchiOBHm_Chr6g0302191
rosa_laevigata RLG00000011188
rosa_multiflora Rmu_co8058424.1_g000001
rosa_roxburghii Rroxscaffold_7G00166010
rosa_rugosa Rorug06G0313000
rosa_samantha Rh6AG425500 Rh6BG477000 Rh6CG438300 Rh6DG425300
rosa_wichuraiana Rw6G036850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 811
AccB1I GGYRCC 1 cut(s) 1402
AccII CGCG 3 cut(s) 620, 1052, 1181
AclWI GGATC 1 cut(s) 1334
AcoI YGGCCR 3 cut(s) 198, 226, 847
AcsI RAATTY 5 cut(s) 321, 404, 428, 614, 975
AfaI GTAC 3 cut(s) 1043, 1079, 1169
AfiI CCNNNNNNNGG 4 cut(s) 83, 119, 259, 676
AgeI ACCGGT 1 cut(s) 676
AgsI TTSAA 5 cut(s) 355, 409, 437, 455, 1387
AjiI CACGTC 2 cut(s) 442, 1255
AjnI CCWGG 3 cut(s) 327, 807, 929
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AleI CACNNNNGTG 1 cut(s) 1487
AloI GAACNNNNNNTCC 2 cut(s) 1334, 1366
AluBI AGCT 8 cut(s) 13, 97, 334, 611, 698, 843, 888, 1299
AluI AGCT 8 cut(s) 13, 97, 334, 611, 698, 843, 888, 1299
Alw21I GWGCWC 3 cut(s) 336, 700, 845
Alw26I GTCTC 5 cut(s) 667, 742, 819, 834, 900
AlwI GGATC 1 cut(s) 1334
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 4 cut(s) 198, 226, 847, 1319
ApeKI GCWGC 1 cut(s) 651
ApoI RAATTY 5 cut(s) 321, 404, 428, 614, 975
ArsI GACNNNNNNTTYG 2 cut(s) 848, 880
AsiGI ACCGGT 1 cut(s) 676
AspS9I GGNCC 1 cut(s) 1319
AsuC2I CCSGG 2 cut(s) 77, 1304
AsuHPI GGTGA 7 cut(s) 206, 235, 377, 862, 883, 1123, 1474
AvaI CYCGRG 1 cut(s) 8
BanI GGYRCC 1 cut(s) 1402
BanII GRGCYC 3 cut(s) 336, 700, 845
BbsI GAAGAC 2 cut(s) 444, 809
Bbv12I GWGCWC 3 cut(s) 336, 700, 845
BbvCI CCTCAGC 1 cut(s) 916
BbvI GCAGC 1 cut(s) 663
BccI CCATC 7 cut(s) 784, 1030, 1150, 1177, 1186, 1233, 1339
BceAI ACGGC 2 cut(s) 361, 705
BciT130I CCWGG 3 cut(s) 329, 809, 931
BcnI CCSGG 2 cut(s) 77, 1304
BcoDI GTCTC 5 cut(s) 667, 742, 819, 834, 900
BfaI CTAG 2 cut(s) 17, 495
BisI GCNGC 5 cut(s) 605, 652, 921, 1053, 1260
BlsI GCNGC 5 cut(s) 606, 653, 922, 1054, 1261
Bme1390I CCNGG 5 cut(s) 77, 329, 809, 931, 1304
BmeT110I CYCGRG 1 cut(s) 8
BmgBI CACGTC 2 cut(s) 442, 1255
BmgT120I GGNCC 1 cut(s) 1319
BmiI GGNNCC 4 cut(s) 1012, 1089, 1320, 1404
BmrFI CCNGG 5 cut(s) 77, 329, 809, 931, 1304
BmrI ACTGGG 1 cut(s) 1225
BmsI GCATC 1 cut(s) 625
BmuI ACTGGG 1 cut(s) 1225
BpiI GAAGAC 2 cut(s) 444, 809
BpmI CTGGAG 3 cut(s) 167, 311, 379
Bpu10I CCTNAGC 2 cut(s) 22, 916
BpuEI CTTGAG 4 cut(s) 44, 260, 859, 965
BpuMI CCSGG 2 cut(s) 77, 1304
BsaI GGTCTC 2 cut(s) 667, 742
BsaJI CCNNGG 4 cut(s) 195, 337, 807, 1322
BsaWI WCCGGW 2 cut(s) 676, 1477
BsaXI ACNNNNNCTCC 4 cut(s) 1025, 1055, 1334, 1364
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 119, 259, 676
Bse118I RCCGGY 4 cut(s) 83, 465, 676, 1084
Bse1I ACTGG 2 cut(s) 377, 1220
BseBI CCWGG 3 cut(s) 329, 809, 931
BseDI CCNNGG 4 cut(s) 195, 337, 807, 1322
BseGI GGATG 4 cut(s) 270, 311, 1161, 1197
BseLI CCNNNNNNNGG 4 cut(s) 83, 119, 259, 676
BseMII CTCAG 5 cut(s) 165, 413, 573, 662, 930
BseNI ACTGG 2 cut(s) 377, 1220
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 1 cut(s) 663
BseYI CCCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 662
Bsh1236I CGCG 3 cut(s) 620, 1052, 1181
Bsh1285I CGRYCG 2 cut(s) 466, 1095
BshFI GGCC 4 cut(s) 200, 228, 849, 1321
BshNI GGYRCC 1 cut(s) 1402
BshTI ACCGGT 1 cut(s) 676
BsiEI CGRYCG 2 cut(s) 466, 1095
BsiHKAI GWGCWC 3 cut(s) 336, 700, 845
BsiHKCI CYCGRG 1 cut(s) 8
BslFI GGGAC 1 cut(s) 1150
BslI CCNNNNNNNGG 4 cut(s) 83, 119, 259, 676
BsmAI GTCTC 5 cut(s) 667, 742, 819, 834, 900
BsmFI GGGAC 1 cut(s) 1150
BsnI GGCC 4 cut(s) 200, 228, 849, 1321
Bso31I GGTCTC 2 cut(s) 667, 742
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 3 cut(s) 336, 700, 845
Bsp1407I TGTACA 1 cut(s) 1041
Bsp143I GATC 4 cut(s) 645, 820, 1092, 1326
BspANI GGCC 4 cut(s) 200, 228, 849, 1321
BspCNI CTCAG 5 cut(s) 166, 414, 574, 663, 929
BspFNI CGCG 3 cut(s) 620, 1052, 1181
BspLI GGNNCC 4 cut(s) 1012, 1089, 1320, 1404
BspPI GGATC 1 cut(s) 1334
BspT107I GGYRCC 1 cut(s) 1402
BspTNI GGTCTC 2 cut(s) 667, 742
BsrFI RCCGGY 4 cut(s) 83, 465, 676, 1084
BsrGI TGTACA 1 cut(s) 1041
BsrI ACTGG 2 cut(s) 377, 1220
BssAI RCCGGY 4 cut(s) 83, 465, 676, 1084
BssECI CCNNGG 4 cut(s) 195, 337, 807, 1322
BssMI GATC 4 cut(s) 645, 820, 1092, 1326
Bst2UI CCWGG 3 cut(s) 329, 809, 931
Bst4CI ACNGT 2 cut(s) 365, 1486
Bst6I CTCTTC 6 cut(s) 776, 898, 987, 1358, 1382, 1406
BstAUI TGTACA 1 cut(s) 1041
BstC8I GCNNGC 3 cut(s) 95, 500, 609
BstDEI CTNAG 6 cut(s) 22, 174, 422, 582, 671, 916
BstEII GGTNACC 1 cut(s) 1480
BstF5I GGATG 4 cut(s) 270, 311, 1161, 1197
BstFNI CGCG 3 cut(s) 620, 1052, 1181
BstKTI GATC 4 cut(s) 648, 823, 1095, 1329
BstMAI GTCTC 5 cut(s) 667, 742, 819, 834, 900
BstMBI GATC 4 cut(s) 645, 820, 1092, 1326
BstMCI CGRYCG 2 cut(s) 466, 1095
BstMWI GCNNNNNNNGC 6 cut(s) 43, 254, 617, 717, 726, 1081
BstNI CCWGG 3 cut(s) 329, 809, 931
BstPI GGTNACC 1 cut(s) 1480
BstSCI CCNGG 5 cut(s) 75, 327, 807, 929, 1302
BstUI CGCG 3 cut(s) 620, 1052, 1181
BstV1I GCAGC 1 cut(s) 663
BstV2I GAAGAC 2 cut(s) 444, 809
BstX2I RGATCY 1 cut(s) 1326
BstXI CCANNNNNNTGG 1 cut(s) 1239
BstYI RGATCY 1 cut(s) 1326
BsuRI GGCC 4 cut(s) 200, 228, 849, 1321
BtgZI GCGATG 1 cut(s) 1196
BtrI CACGTC 2 cut(s) 442, 1255
BtsCI GGATG 4 cut(s) 270, 311, 1161, 1197
BtsI GCAGTG 1 cut(s) 1167
BtsIMutI CAGTG 2 cut(s) 171, 1167
Cac8I GCNNGC 3 cut(s) 95, 500, 609
Cfr10I RCCGGY 4 cut(s) 83, 465, 676, 1084
Cfr13I GGNCC 1 cut(s) 1319
CsiI ACCWGGT 1 cut(s) 929
Csp6I GTAC 3 cut(s) 1042, 1078, 1168
CspAI ACCGGT 1 cut(s) 676
CviAII CATG 4 cut(s) 149, 165, 1069, 1172
CviQI GTAC 3 cut(s) 1042, 1078, 1168
DdeI CTNAG 6 cut(s) 22, 174, 422, 582, 671, 916
DpnI GATC 4 cut(s) 647, 822, 1094, 1328
DpnII GATC 4 cut(s) 645, 820, 1092, 1326
DrdI GACNNNNNNGTC 1 cut(s) 811
DseDI GACNNNNNNGTC 1 cut(s) 811
EaeI YGGCCR 3 cut(s) 198, 226, 847
Eam1104I CTCTTC 6 cut(s) 776, 898, 987, 1358, 1382, 1406
EarI CTCTTC 6 cut(s) 776, 898, 987, 1358, 1382, 1406
EciI GGCGGA 1 cut(s) 1277
Ecl136II GAGCTC 3 cut(s) 334, 698, 843
Eco24I GRGCYC 3 cut(s) 336, 700, 845
Eco31I GGTCTC 2 cut(s) 667, 742
Eco53kI GAGCTC 3 cut(s) 334, 698, 843
Eco88I CYCGRG 1 cut(s) 8
Eco91I GGTNACC 1 cut(s) 1480
EcoICRI GAGCTC 3 cut(s) 334, 698, 843
EcoO65I GGTNACC 1 cut(s) 1480
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 3 cut(s) 327, 807, 929
EcoT38I GRGCYC 3 cut(s) 336, 700, 845
FaeI CATG 4 cut(s) 152, 168, 1072, 1175
FaiI YATR 8 cut(s) 109, 150, 166, 473, 1020, 1070, 1173, 1204
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 1150
FatI CATG 4 cut(s) 148, 164, 1068, 1171
FauI CCCGC 3 cut(s) 262, 1089, 1309
Fnu4HI GCNGC 5 cut(s) 605, 652, 921, 1053, 1260
FokI GGATG 4 cut(s) 257, 298, 1168, 1204
FriOI GRGCYC 3 cut(s) 336, 700, 845
Fsp4HI GCNGC 5 cut(s) 605, 652, 921, 1053, 1260
FspBI CTAG 2 cut(s) 17, 495
GluI GCNGC 5 cut(s) 605, 652, 921, 1053, 1260
GsaI CCCAGC 1 cut(s) 134
GsuI CTGGAG 3 cut(s) 167, 311, 379
HaeIII GGCC 4 cut(s) 200, 228, 849, 1321
Hin1II CATG 4 cut(s) 152, 168, 1072, 1175
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 7 cut(s) 159, 178, 308, 561, 705, 812, 1289
HphI GGTGA 7 cut(s) 206, 235, 377, 862, 883, 1123, 1474
Hpy166II GTNNAC 6 cut(s) 171, 448, 1042, 1044, 1114, 1177
Hpy188I TCNGA 7 cut(s) 715, 742, 856, 927, 1003, 1092, 1229
Hpy188III TCNNGA 9 cut(s) 8, 184, 277, 382, 512, 670, 818, 838, 967
Hpy8I GTNNAC 6 cut(s) 171, 448, 1042, 1044, 1114, 1177
Hpy99I CGWCG 6 cut(s) 347, 365, 860, 968, 1121, 1124
HpyAV CCTTC 8 cut(s) 553, 602, 606, 1024, 1144, 1279, 1333, 1459
HpyCH4III ACNGT 2 cut(s) 365, 1486
HpyCH4IV ACGT 4 cut(s) 441, 963, 1122, 1254
HpyCH4V TGCA 2 cut(s) 643, 1357
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 254, 617, 717, 726, 1081
HpyF3I CTNAG 6 cut(s) 22, 174, 422, 582, 671, 916
HpySE526I ACGT 4 cut(s) 441, 963, 1122, 1254
Hsp92II CATG 4 cut(s) 152, 168, 1072, 1175
Kzo9I GATC 4 cut(s) 645, 820, 1092, 1326
LmnI GCTCC 8 cut(s) 42, 102, 331, 339, 848, 1010, 1195, 1246
Lsp1109I GCAGC 1 cut(s) 663
LweI GCATC 1 cut(s) 625
MabI ACCWGGT 1 cut(s) 929
MaeI CTAG 2 cut(s) 17, 495
MaeII ACGT 4 cut(s) 441, 963, 1122, 1254
MaeIII GTNAC 4 cut(s) 365, 868, 1250, 1480
MalI GATC 4 cut(s) 647, 822, 1094, 1328
MboI GATC 4 cut(s) 645, 820, 1092, 1326
MflI RGATCY 1 cut(s) 1326
MhlI GDGCHC 3 cut(s) 336, 700, 845
MluCI AATT 5 cut(s) 321, 404, 428, 614, 975
MlyI GAGTC 4 cut(s) 153, 317, 821, 1283
MmeI TCCRAC 3 cut(s) 879, 1224, 1252
MseI TTAA 2 cut(s) 1395, 1501
MslI CAYNNNNRTG 1 cut(s) 1487
MspA1I CMGCKG 2 cut(s) 257, 920
MspR9I CCNGG 5 cut(s) 77, 329, 809, 931, 1304
MvaI CCWGG 3 cut(s) 329, 809, 931
MvnI CGCG 3 cut(s) 620, 1052, 1181
MwoI GCNNNNNNNGC 6 cut(s) 43, 254, 617, 717, 726, 1081
NciI CCSGG 2 cut(s) 77, 1304
NdeII GATC 4 cut(s) 645, 820, 1092, 1326
NlaIII CATG 4 cut(s) 152, 168, 1072, 1175
NlaIV GGNNCC 4 cut(s) 1012, 1089, 1320, 1404
NmeAIII GCCGAG 3 cut(s) 176, 318, 1347
NmuCI GTSAC 4 cut(s) 365, 868, 1250, 1480
OliI CACNNNNGTG 1 cut(s) 1487
PaeR7I CTCGAG 1 cut(s) 8
PcsI WCGNNNNNNNCGW 3 cut(s) 438, 864, 969
PfeI GAWTC 3 cut(s) 178, 561, 705
PflFI GACNNNGTC 1 cut(s) 878
PfoI TCCNGGA 1 cut(s) 327
PinAI ACCGGT 1 cut(s) 676
PkrI GCNGC 5 cut(s) 606, 653, 922, 1054, 1261
Ple19I CGATCG 1 cut(s) 1095
PleI GAGTC 4 cut(s) 153, 316, 820, 1283
PpsI GAGTC 4 cut(s) 153, 316, 820, 1283
Psp124BI GAGCTC 3 cut(s) 336, 700, 845
Psp6I CCWGG 3 cut(s) 327, 807, 929
PspEI GGTNACC 1 cut(s) 1480
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 3 cut(s) 327, 807, 929
PspN4I GGNNCC 4 cut(s) 1012, 1089, 1320, 1404
PspPI GGNCC 1 cut(s) 1319
PsuI RGATCY 1 cut(s) 1326
PsyI GACNNNGTC 1 cut(s) 878
PvuI CGATCG 1 cut(s) 1095
RsaI GTAC 3 cut(s) 1043, 1079, 1169
RsaNI GTAC 3 cut(s) 1042, 1078, 1168
RseI CAYNNNNRTG 1 cut(s) 1487
SacI GAGCTC 3 cut(s) 336, 700, 845
SaqAI TTAA 2 cut(s) 1395, 1501
SatI GCNGC 5 cut(s) 605, 652, 921, 1053, 1260
Sau3AI GATC 4 cut(s) 645, 820, 1092, 1326
Sau96I GGNCC 1 cut(s) 1319
SchI GAGTC 4 cut(s) 153, 317, 821, 1283
ScrFI CCNGG 5 cut(s) 77, 329, 809, 931, 1304
SduI GDGCHC 3 cut(s) 336, 700, 845
SexAI ACCWGGT 1 cut(s) 929
SfaNI GCATC 1 cut(s) 625
Sfr274I CTCGAG 1 cut(s) 8
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 1487
SmlI CTYRAG 5 cut(s) 8, 59, 275, 838, 980
SmoI CTYRAG 5 cut(s) 8, 59, 275, 838, 980
Sse9I AATT 5 cut(s) 321, 404, 428, 614, 975
SspMI CTAG 2 cut(s) 17, 495
SstI GAGCTC 3 cut(s) 336, 700, 845
StyD4I CCNGG 5 cut(s) 75, 327, 807, 929, 1302
TaaI ACNGT 2 cut(s) 365, 1486
TaiI ACGT 4 cut(s) 444, 966, 1125, 1257
TaqI TCGA 4 cut(s) 9, 546, 966, 1095
TasI AATT 5 cut(s) 321, 404, 428, 614, 975
TatI WGTACW 1 cut(s) 1041
TauI GCSGC 4 cut(s) 607, 923, 1055, 1262
TfiI GAWTC 3 cut(s) 178, 561, 705
Tru1I TTAA 2 cut(s) 1395, 1501
Tru9I TTAA 2 cut(s) 1395, 1501
TscAI CASTG 2 cut(s) 178, 1167
TseFI GTSAC 4 cut(s) 365, 868, 1250, 1480
TseI GCWGC 1 cut(s) 651
Tsp45I GTSAC 4 cut(s) 365, 868, 1250, 1480
TspDTI ATGAA 5 cut(s) 114, 815, 1038, 1376, 1478
TspGWI ACGGA 2 cut(s) 226, 1023
TspRI CASTG 2 cut(s) 178, 1167
Tth111I GACNNNGTC 1 cut(s) 878
XapI RAATTY 5 cut(s) 321, 404, 428, 614, 975
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 2 cut(s) 17, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.