MD08G1017600.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011850
Physical Location & Seq
Forward (+)
1338714 .. 1342142
3429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1017600.v1.1.491

Sequence Viewer

Length: 3429 bp
ATGGCGACGCCCGCTTTCCTCTTCCTCATAACAATTGCTATCCCAACTATTTATTTCCCCGCCACCTTAACGGCGGCTCAACGGCCGAGTGCGGAAACGGTACTCTCTGAGATCCAAGCTTTAACGGCGTTTAAGCGTAACCTCCACGACCCTCTTGGCGCTCTCGACGGCTGGGATACGTCCACGCCGTCGGCCCCGTGTGACTGGCGCGGCGTCGTCTGTTACAACAACCGAGTGCGGGAGCTCCGCCTGCCTCGTCTCCACCTTGGCGGCCGAATTACTGACCACCTCGCCAGCCTCCGCGAACTCCGCAAGCTCAGCCTCTACAGCAACAACTTCAACGGCGCCATACCTCCTTCTCTCTCCCTATGCGCTCTCTTACGGGCTGTTTACCTGCACGGCAACTCGCTCACCGGAACTCTTCCACCGGCGATTTTCAACCTCACCAACCTCCAAATCCTCAACGTCGCTCACAATTTCCTCTCTGGAAAAATCTCCGGCGACATTCCGGTGAGGCTGCGTTACCTCGACCTCTCGTCGAACACATTCTCTGGTGAGATTCCGACGAACTTCTCCGCCGATTCAAGCCTCCAGCTTATCAATCTCTCGTTCAATCGGTTCTCTGGCAGTGTCCCGGTGACTATCGGACGTCTCAAGAATCTTGAGTATCTGTGGTTGGACTCGAACCAGCTGCACGGTACTCTGCCTTCGGCGATTTCCAACTGCTCCTCACTCGTACATTTGAGCGCCGACGACAATGCGCTGAGAGGTCTGATTCCGACGACGATAGGAGCGATGCCGAAGCTTCAGGTGGTGTCGCTGTCGCGGAACGAGCTGTCAGGGGCGGTCCCCACGTCGTTGTTGTGCAGTACTGCAAACAACGACTCGTCGTTGAGGATTGTGGAGCTCGGATTCAATGCGCTCACGAGCATCGCTGAGCCACCAGATATCTCAAAATGTTCGAGTGCATTGGGGGTTTTGGGCTTAAAATCCAATCAGATAAGTGGCTCCTTCCCATATTGGTTGACAAACGTGACGACAATTATGAAAGTTCTAGATCTTTCTGGAAACTTGTTTTCGGGTCAATTGCCGCCGGAGATTGACAATCTTCTCCGGTTGGAGGAGTTTCGGGTGGCAAATAACTCGCTGTCCGGTGAAATTCCTGGTGAGATCGTGAAATGTAGCTTGTTGCAGGTCCTTGATGTTGAAGGAAATAGGTTTGCAGGTCAAGTTCCACTTTTTTTAGCAGGAATGAGGAGCTTGAAGGTGTTGTCTTTGGGTGGGAATTTATTCTGCGGTCCCATTCCGGCGAGTTTAGGAGCGCTTTCGCAGCTCGAATCTTTGGATTTGAGCAACAATAATCTGACCGGGAAAGTTCCTGATGAGCTAATGATGCAGCTAAGCAACTTGACTAGTTTGAATCTGAGTAATAACAAGTTTTCTGGTGAACTTATTAATGTTGGAGAGTTGAAGAGCTTGCAGGTTTTGAACTTGAGCAATTGTGGGTTTTCGGGGAGTGTTCCTCCGAGCATTGGTGGTTTGATGAAGCTTGAAACACTTGATTTGAGTAAACAGAAGCTTTCGGGGGAGTTGCCGGTTCAGATTTTCGGGTTGCCAAATTTACAACTGGTTGCTGTGCAGGAAAATCACTTATCAGGAGATGTCCCTCAAGGTTTTAGTAGTTTGATTAGTTTGAACTATCTTAACCTATCTTACAATGCTTTTACTGGTGATATTCCTACAACTTATGGATTTCTCAACTCATTAGTTGTTCTATCATTGTCTGGAAATCGCATTTCGGGTACGATCCCACAAGAGCTTGGAGATAGTTTGAATCTTGGAGTGCTCGAGCTTCGTTCTAATCAGTTAGGTGGCAAAATTCCAGGCGATATCTCTCGCCTATCCAATTTGAAAGAGCTCAATCTAGGTCATAATAAGTTGACGGGTGAAATCCCAAAGGAGATTTCCAATTGCTCTGCTTTGACTTCTCTGTTGTTAGGTGGGAATCGACTTTCAGGTCAAATACCACACTCATTGCCTAAGTTATTGAACATCACAGTGTTGGATCTTTCCTCTAACAATTTGAGTGGCAGCATCCCGGAAGATCTTTCGCTCATCATCCCTCACTTGAAATACCTTAACTTATCAAACAACAACCTTGCTGGAGAGATTCCGAAGCCACTGGGTTCTCAGTTCAACGATCCTTCTGTGTTTGCAAGGAATGGAGATTTATGTGGGAAACCATTGGATAGAGAATGTGCAGACGTGAGAAGGAGAAAAAGAAACAGGTTGACCCTATTGATTGCTGTGGCTGTGGGAGGAGCTTTTCTTCTGGCGTTGTGTTGCTGTGGCTATATTTACAGCCTACTGCGATGGCGGACAAAACTTAGAGAAACAGTAATCGGGGGGCAGAAGAAGCGAACTAGCCCTCGAACAAGCTCAGGGGATAAAAGCAGCCGGGGAAGTGGCGATGGAGGGCCAAAACTTGTGATGTTCAATAGCAAGATCACGTATGCAGAGGCATTGGAAGCAACGAGACAATTCGAAGAGGAAAATGTGTTAAGCAGGGGAAGATATGGACTGGTATTCAAGGCCACATTTCAAGATGGAATGGTGCTCGCAATTCGACGGCTTCCCGATGGTTTTCTTGATGAAGGCAGCTTCCGCAAAGAAGCTGAAGATCTTGGCAAGGTGAAGCATCGAAACCTAACAGTTTTACGAGGCTATTATGCCGGTCCTCCTGACGTGAGGCTCCTTGTGTACGATTACATGCCCAATGGAAACTTAAGCGCTCTTCTACAAGAGGCCTCTCACCAAGACGGGCATGTACTAAATTGGCCAATGAGGCACCTCATAGCACTCGGCATTGCTCGTGGATTAGCCTACCTGCACTCTGCCTCAATGGTACACGGCGATGTGAAGCCGCAGAACGTTCTTTTTGACGCTGATTTCGAAGCCCATTTATCCGAATTTGGTCTAGACCGGGTAACCATAGCCACACCAGCAAAAGCTTCCTCGTCTTCAACCACGATCGGCTCCTTAGGCTACGTGTCCCCCGAAGCTGCATTAACAGGGCAAGCAACGAAAGAAGCCGATGTGTATAGTTTTGGGATTGTATTGATGGAGATACTGACCGGGAGGAATCCGGTGACGTTTACTCAAGACGAAGACATTGTGAAGTGGGTGAAGAAGCAACTTCAGAACGGTCAAGTTTCCGAGCTGCTCGAGCCCGGGCTGCTCGAACTTGACCCGGAATCGTCAGACTGGGAAGAGTTCTTGCTGGGAGTGAAAGTGGGGCTTCTTTGCACGGCTCCTGATCCGCTTGATCGACCATCCATTGCCGACATTGTGTTCATGCTTGAAGGTTGCAGAGTCGGACCCGACATCCCGTCCTCGGCTGATCCGACCTCTCTGCCTTCGCCAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1143

Amino Acids

123.22

Weight (kDa)

6.12

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 74 1.5e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 172 - 231 1.9e-06 Leucine rich repeat
LRR_14 PF23598 172 - 301 5.2e-07 Leucine-rich repeat region
LRR_14 PF23598 348 - 474 1.3e-08 Leucine-rich repeat region
LRR_8 PF13855 419 - 480 4.3e-06 Leucine rich repeat
LRR_14 PF23598 482 - 571 1.9e-09 Leucine-rich repeat region
LRR_8 PF13855 539 - 599 6.4e-08 Leucine rich repeat
LRR_8 PF13855 612 - 671 5.3e-08 Leucine rich repeat
Pkinase PF00069 848 - 1068 5.6e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 851 - 1068 1.3e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75640
fragaria_vesca FvH4_2g39080
malus_domestica MD08G1017600.v1.1 MD08G1017800.v1.1 MD15G1016500.v1.1
prunus_persica Prupe.1G370300_v2.0.a1
pyrus_communis pycom08g01540 pycom15g01450 pycom15g01460
rosa_chinensis RchiOBHm_Chr6g0302191
rosa_laevigata RLG00000011188
rosa_multiflora Rmu_co8058424.1_g000001
rosa_roxburghii Rroxscaffold_7G00166010
rosa_rugosa Rorug06G0313000
rosa_samantha Rh6AG425500 Rh6BG477000 Rh6CG438300 Rh6DG425300
rosa_wichuraiana Rw6G036850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2016
AatII GACGTC 1 cut(s) 652
Acc36I ACCTGC 5 cut(s) 402, 1183, 1214, 1471, 2895
AccB1I GGYRCC 2 cut(s) 344, 2847
AccII CGCG 3 cut(s) 210, 303, 826
AclI AACGTT 1 cut(s) 2931
AclWI GGATC 6 cut(s) 106, 1801, 2073, 2195, 3311, 3395
AcoI YGGCCR 3 cut(s) 83, 271, 2837
AcsI RAATTY 5 cut(s) 1158, 1285, 1618, 1878, 2969
AcuI CTGAAG 3 cut(s) 791, 2697, 3182
AcyI GRCGYC 4 cut(s) 8, 213, 345, 649
AfaI GTAC 8 cut(s) 102, 700, 738, 871, 1804, 2762, 2829, 2907
AfeI AGCGCT 2 cut(s) 1323, 2791
AfiI CCNNNNNNNGG 4 cut(s) 238, 1120, 1532, 3394
AflII CTTAAG 1 cut(s) 2785
AflIII ACRYGT 1 cut(s) 3048
AhdI GACNNNNNGTC 2 cut(s) 535, 3388
AhlI ACTAGT 1 cut(s) 1412
AjiI CACGTC 3 cut(s) 855, 2266, 2746
AjnI CCWGG 2 cut(s) 1162, 1882
AjuI GAANNNNNNNTTGG 2 cut(s) 1948, 1980
Alw21I GWGCWC 5 cut(s) 246, 909, 1848, 1920, 2619
Alw26I GTCTC 3 cut(s) 263, 656, 2530
AlwI GGATC 6 cut(s) 106, 1801, 2073, 2195, 3311, 3395
Ama87I CYCGRG 3 cut(s) 1847, 3224, 3230
Aor51HI AGCGCT 2 cut(s) 1323, 2791
AoxI GGCC 7 cut(s) 83, 192, 271, 2477, 2592, 2805, 2837
ApoI RAATTY 5 cut(s) 1158, 1285, 1618, 1878, 2969
ArsI GACNNNNNNTTYG 2 cut(s) 2933, 2965
AseI ATTAAT 1 cut(s) 1455
Asp700I GAANNNNTTC 2 cut(s) 545, 1289
AspLEI GCGC 9 cut(s) 161, 210, 347, 374, 749, 763, 922, 1324, 2792
AspS9I GGNCC 7 cut(s) 193, 847, 1195, 1298, 2477, 2735, 3377
AsuC2I CCSGG 9 cut(s) 635, 1369, 2099, 2459, 2984, 3136, 3231, 3232, 3251
AsuII TTCGAA 2 cut(s) 2544, 2952
AvaI CYCGRG 3 cut(s) 1847, 3224, 3230
AvaII GGWCC 5 cut(s) 847, 1195, 1298, 2735, 3377
AxyI CCTNAGG 1 cut(s) 3040
BalI TGGCCA 1 cut(s) 2839
BanI GGYRCC 2 cut(s) 344, 2847
BanII GRGCYC 4 cut(s) 246, 909, 1920, 3231
BarI GAAGNNNNNNTAC 2 cut(s) 691, 723
BauI CACGAG 2 cut(s) 925, 2871
BbsI GAAGAC 2 cut(s) 3012, 3174
Bbv12I GWGCWC 5 cut(s) 246, 909, 1848, 1920, 2619
BccI CCATC 6 cut(s) 2367, 2465, 2600, 2633, 3115, 3340
BcgI CGANNNNNNTGC 8 cut(s) 673, 707, 740, 774, 1125, 1159, 3394, 3428
BciT130I CCWGG 2 cut(s) 1164, 1884
BciVI GTATCC 1 cut(s) 169
BcnI CCSGG 9 cut(s) 635, 1369, 2099, 2459, 2984, 3136, 3231, 3232, 3251
BcoDI GTCTC 3 cut(s) 263, 656, 2530
BcuI ACTAGT 1 cut(s) 1412
BfaI CTAG 5 cut(s) 1055, 1413, 1925, 2424, 2978
BfmI CTRYAG 1 cut(s) 325
BfoI RGCGCY 5 cut(s) 162, 348, 750, 1325, 2793
BfrI CTTAAG 1 cut(s) 2785
BfuAI ACCTGC 5 cut(s) 402, 1183, 1214, 1471, 2895
BfuI GTATCC 1 cut(s) 169
BglI GCCNNNNNGGC 1 cut(s) 2845
BglII AGATCT 3 cut(s) 1057, 2104, 2680
BlpI GCTNAGC 3 cut(s) 317, 936, 1400
BmcAI AGTACT 1 cut(s) 871
Bme18I GGWCC 5 cut(s) 847, 1195, 1298, 2735, 3377
BmeRI GACNNNNNGTC 2 cut(s) 535, 3388
BmeT110I CYCGRG 3 cut(s) 1847, 3224, 3230
BmgBI CACGTC 3 cut(s) 855, 2266, 2746
BmgT120I GGNCC 7 cut(s) 193, 847, 1195, 1298, 2477, 2735, 3377
BmrI ACTGGG 2 cut(s) 2192, 3274
BmsI GCATC 5 cut(s) 786, 939, 1383, 2103, 2707
BmuI ACTGGG 2 cut(s) 2192, 3274
BpiI GAAGAC 2 cut(s) 3012, 3174
BplI GAGNNNNNCTC 2 cut(s) 1507, 1539
BpmI CTGGAG 2 cut(s) 575, 2184
Bpu10I CCTNAGC 1 cut(s) 2440
Bpu1102I GCTNAGC 3 cut(s) 317, 936, 1400
Bpu14I TTCGAA 2 cut(s) 2544, 2952
BpuEI CTTGAG 5 cut(s) 638, 683, 1513, 1653, 3144
BpuMI CCSGG 9 cut(s) 635, 1369, 2099, 2459, 2984, 3136, 3231, 3232, 3251
BsaAI YACGTR 2 cut(s) 2511, 3049
BsaHI GRCGYC 4 cut(s) 8, 213, 345, 649
BsaJI CCNNGG 4 cut(s) 265, 2458, 3230, 3393
BsaWI WCCGGW 5 cut(s) 413, 508, 1113, 1151, 3145
BsaXI ACNNNNNCTCC 2 cut(s) 3276, 3306
Bsc4I CCNNNNNNNGG 4 cut(s) 238, 1120, 1532, 3394
Bse118I RCCGGY 3 cut(s) 427, 1594, 2732
Bse1I ACTGG 6 cut(s) 209, 1632, 1732, 2187, 2586, 3269
Bse21I CCTNAGG 1 cut(s) 3040
Bse3DI GCAATG 3 cut(s) 2033, 2865, 3336
BseBI CCWGG 2 cut(s) 1164, 1884
BseDI CCNNGG 4 cut(s) 265, 2458, 3230, 3393
BseGI GGATG 4 cut(s) 2094, 2118, 3332, 3384
BseLI CCNNNNNNNGG 4 cut(s) 238, 1120, 1532, 3394
BseMI GCAATG 3 cut(s) 2033, 2865, 3336
BseMII CTCAG 7 cut(s) 99, 331, 755, 927, 1415, 2204, 2454
BseNI ACTGG 6 cut(s) 209, 1632, 1732, 2187, 2586, 3269
BseRI GAGGAG 4 cut(s) 718, 1136, 1270, 2334
BseX3I CGGCCG 2 cut(s) 83, 271
BseYI CCCAGC 2 cut(s) 171, 3280
BsgI GTGCAG 6 cut(s) 380, 677, 886, 1658, 2280, 2873
Bsh1236I CGCG 3 cut(s) 210, 303, 826
Bsh1285I CGRYCG 3 cut(s) 86, 274, 3033
BshFI GGCC 7 cut(s) 85, 194, 273, 2479, 2594, 2807, 2839
BshNI GGYRCC 2 cut(s) 344, 2847
BsiEI CGRYCG 3 cut(s) 86, 274, 3033
BsiHKAI GWGCWC 5 cut(s) 246, 909, 1848, 1920, 2619
BsiHKCI CYCGRG 3 cut(s) 1847, 3224, 3230
BslFI GGGAC 5 cut(s) 617, 833, 1284, 1649, 3037
BslI CCNNNNNNNGG 4 cut(s) 238, 1120, 1532, 3394
BsmAI GTCTC 3 cut(s) 263, 656, 2530
BsmBI CGTCTC 2 cut(s) 263, 656
BsmFI GGGAC 5 cut(s) 617, 833, 1284, 1649, 3037
BsnI GGCC 7 cut(s) 85, 194, 273, 2479, 2594, 2807, 2839
BsoBI CYCGRG 3 cut(s) 1847, 3224, 3230
Bsp119I TTCGAA 2 cut(s) 2544, 2952
Bsp1286I GDGCHC 6 cut(s) 246, 909, 1848, 1920, 2619, 3231
Bsp1720I GCTNAGC 3 cut(s) 317, 936, 1400
BspANI GGCC 7 cut(s) 85, 194, 273, 2479, 2594, 2807, 2839
BspCNI CTCAG 7 cut(s) 100, 330, 756, 928, 1416, 2203, 2453
BspFNI CGCG 3 cut(s) 210, 303, 826
BspMI ACCTGC 5 cut(s) 402, 1183, 1214, 1471, 2895
BspPI GGATC 6 cut(s) 106, 1801, 2073, 2195, 3311, 3395
BspQI GCTCTTC 2 cut(s) 1466, 2799
BspT104I TTCGAA 2 cut(s) 2544, 2952
BspT107I GGYRCC 2 cut(s) 344, 2847
BspTI CTTAAG 1 cut(s) 2785
BsrDI GCAATG 3 cut(s) 2033, 2865, 3336
BsrFI RCCGGY 3 cut(s) 427, 1594, 2732
BsrI ACTGG 6 cut(s) 209, 1632, 1732, 2187, 2586, 3269
BssAI RCCGGY 3 cut(s) 427, 1594, 2732
BssECI CCNNGG 4 cut(s) 265, 2458, 3230, 3393
BssNI GRCGYC 4 cut(s) 8, 213, 345, 649
BssSI CACGAG 2 cut(s) 925, 2871
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 2 cut(s) 925, 2871
Bst2UI CCWGG 2 cut(s) 1164, 1884
Bst4CI ACNGT 6 cut(s) 100, 698, 2059, 2398, 2713, 3206
Bst6I CTCTTC 6 cut(s) 26, 426, 1466, 2541, 2799, 3264
BstACI GRCGYC 4 cut(s) 8, 213, 345, 649
BstAFI CTTAAG 1 cut(s) 2785
BstBAI YACGTR 2 cut(s) 2511, 3049
BstBI TTCGAA 2 cut(s) 2544, 2952
BstC8I GCNNGC 8 cut(s) 12, 251, 295, 314, 1478, 2619, 3078, 3423
BstEII GGTNACC 1 cut(s) 2986
BstF5I GGATG 4 cut(s) 2094, 2118, 3332, 3384
BstFNI CGCG 3 cut(s) 210, 303, 826
BstH2I RGCGCY 5 cut(s) 162, 348, 750, 1325, 2793
BstHHI GCGC 9 cut(s) 161, 210, 347, 374, 749, 763, 922, 1324, 2792
BstMAI GTCTC 3 cut(s) 263, 656, 2530
BstMCI CGRYCG 3 cut(s) 86, 274, 3033
BstNI CCWGG 2 cut(s) 1164, 1884
BstNSI RCATGY 2 cut(s) 2773, 2828
BstPI GGTNACC 1 cut(s) 2986
BstSFI CTRYAG 1 cut(s) 325
BstUI CGCG 3 cut(s) 210, 303, 826
BstV2I GAAGAC 2 cut(s) 3012, 3174
BstX2I RGATCY 5 cut(s) 111, 1057, 2065, 2104, 2680
BstYI RGATCY 5 cut(s) 111, 1057, 2065, 2104, 2680
BstZI CGGCCG 2 cut(s) 83, 271
Bsu36I CCTNAGG 1 cut(s) 3040
BsuI GTATCC 1 cut(s) 169
BsuRI GGCC 7 cut(s) 85, 194, 273, 2479, 2594, 2807, 2839
BtgZI GCGATG 5 cut(s) 809, 916, 2386, 2484, 2928
BtrI CACGTC 3 cut(s) 855, 2266, 2746
BtsCI GGATG 4 cut(s) 2094, 2118, 3332, 3384
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 3 cut(s) 634, 2064, 2180
BveI ACCTGC 5 cut(s) 402, 1183, 1214, 1471, 2895
Cac8I GCNNGC 8 cut(s) 12, 251, 295, 314, 1478, 2619, 3078, 3423
CfoI GCGC 9 cut(s) 161, 210, 347, 374, 749, 763, 922, 1324, 2792
Cfr10I RCCGGY 3 cut(s) 427, 1594, 2732
Cfr13I GGNCC 7 cut(s) 193, 847, 1195, 1298, 2477, 2735, 3377
Cfr9I CCCGGG 1 cut(s) 3230
CseI GACGC 3 cut(s) 16, 202, 2951
Csp6I GTAC 8 cut(s) 101, 699, 737, 870, 1803, 2761, 2828, 2906
CspCI CAANNNNNGTGG 4 cut(s) 2016, 2051, 2068, 2103
CviAII CATG 3 cut(s) 2770, 2825, 3355
CviQI GTAC 8 cut(s) 101, 699, 737, 870, 1803, 2761, 2828, 2906
DinI GGCGCC 1 cut(s) 346
DrdI GACNNNNNNGTC 1 cut(s) 2016
DriI GACNNNNNGTC 2 cut(s) 535, 3388
DseDI GACNNNNNNGTC 1 cut(s) 2016
EaeI YGGCCR 3 cut(s) 83, 271, 2837
EagI CGGCCG 2 cut(s) 83, 271
Eam1104I CTCTTC 6 cut(s) 26, 426, 1466, 2541, 2799, 3264
Eam1105I GACNNNNNGTC 2 cut(s) 535, 3388
EarI CTCTTC 6 cut(s) 26, 426, 1466, 2541, 2799, 3264
EciI GGCGGA 3 cut(s) 236, 565, 2392
Ecl136II GAGCTC 3 cut(s) 244, 907, 1918
EclXI CGGCCG 2 cut(s) 83, 271
Eco130I CCWWGG 1 cut(s) 265
Eco147I AGGCCT 1 cut(s) 2807
Eco24I GRGCYC 4 cut(s) 246, 909, 1920, 3231
Eco32I GATATC 2 cut(s) 949, 1891
Eco47I GGWCC 5 cut(s) 847, 1195, 1298, 2735, 3377
Eco47III AGCGCT 2 cut(s) 1323, 2791
Eco52I CGGCCG 2 cut(s) 83, 271
Eco53kI GAGCTC 3 cut(s) 244, 907, 1918
Eco57I CTGAAG 3 cut(s) 791, 2697, 3182
Eco81I CCTNAGG 1 cut(s) 3040
Eco88I CYCGRG 3 cut(s) 1847, 3224, 3230
Eco91I GGTNACC 1 cut(s) 2986
EcoICRI GAGCTC 3 cut(s) 244, 907, 1918
EcoO109I RGGNCCY 1 cut(s) 1195
EcoO65I GGTNACC 1 cut(s) 2986
EcoRII CCWGG 2 cut(s) 1162, 1882
EcoRV GATATC 2 cut(s) 949, 1891
EcoT14I CCWWGG 1 cut(s) 265
EcoT38I GRGCYC 4 cut(s) 246, 909, 1920, 3231
EgeI GGCGCC 1 cut(s) 346
EheI GGCGCC 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 265
Esp3I CGTCTC 2 cut(s) 263, 656
FaeI CATG 3 cut(s) 2773, 2828, 3358
FalI AAGNNNNNCTT 4 cut(s) 1221, 1253, 3282, 3314
FaqI GGGAC 5 cut(s) 617, 833, 1284, 1649, 3037
FatI CATG 3 cut(s) 2769, 2824, 3354
FauI CCCGC 3 cut(s) 19, 67, 231
FokI GGATG 4 cut(s) 2081, 2105, 3319, 3371
FriOI GRGCYC 4 cut(s) 246, 909, 1920, 3231
FspBI CTAG 5 cut(s) 1055, 1413, 1925, 2424, 2978
GlaI GCGC 9 cut(s) 160, 209, 346, 373, 748, 762, 921, 1323, 2791
GsaI CCCAGC 2 cut(s) 175, 3284
GsuI CTGGAG 2 cut(s) 575, 2184
HaeII RGCGCY 5 cut(s) 162, 348, 750, 1325, 2793
HaeIII GGCC 7 cut(s) 85, 194, 273, 2479, 2594, 2807, 2839
HgaI GACGC 3 cut(s) 16, 202, 2951
HhaI GCGC 9 cut(s) 161, 210, 347, 374, 749, 763, 922, 1324, 2792
Hin1I GRCGYC 4 cut(s) 8, 213, 345, 649
Hin1II CATG 3 cut(s) 2773, 2828, 3358
Hin6I GCGC 9 cut(s) 159, 208, 345, 372, 747, 761, 920, 1322, 2790
HinP1I GCGC 9 cut(s) 159, 208, 345, 372, 747, 761, 920, 1322, 2790
HincII GTYRAC 3 cut(s) 1026, 1941, 2292
HindII GTYRAC 3 cut(s) 1026, 1941, 2292
HindIII AAGCTT 5 cut(s) 117, 803, 1547, 1577, 3009
HpyCH4III ACNGT 6 cut(s) 100, 698, 2059, 2398, 2713, 3206
Hsp92I GRCGYC 4 cut(s) 8, 213, 345, 649
Hsp92II CATG 3 cut(s) 2773, 2828, 3358
HspAI GCGC 9 cut(s) 159, 208, 345, 372, 747, 761, 920, 1322, 2790
KasI GGCGCC 1 cut(s) 344
LguI GCTCTTC 2 cut(s) 1466, 2799
LweI GCATC 5 cut(s) 786, 939, 1383, 2103, 2707
MaeI CTAG 5 cut(s) 1055, 1413, 1925, 2424, 2978
MaeIII GTNAC 8 cut(s) 137, 200, 221, 521, 637, 1033, 2986, 3148
MfeI CAATTG 4 cut(s) 33, 1085, 1498, 1969
MflI RGATCY 5 cut(s) 111, 1057, 2065, 2104, 2680
MhlI GDGCHC 6 cut(s) 246, 909, 1848, 1920, 2619, 3231
MlsI TGGCCA 1 cut(s) 2839
MluNI TGGCCA 1 cut(s) 2839
Mly113I GGCGCC 1 cut(s) 345
MlyI GAGTC 3 cut(s) 674, 878, 3381
MmeI TCCRAC 9 cut(s) 587, 657, 744, 803, 1098, 1441, 2043, 3355, 3428
Mox20I TGGCCA 1 cut(s) 2839
MroXI GAANNNNTTC 2 cut(s) 545, 1289
MscI TGGCCA 1 cut(s) 2839
MslI CAYNNNNRTG 3 cut(s) 509, 2057, 2913
Msp20I TGGCCA 1 cut(s) 2839
MspA1I CMGCKG 1 cut(s) 691
MspCI CTTAAG 1 cut(s) 2785
MunI CAATTG 4 cut(s) 33, 1085, 1498, 1969
MvaI CCWGG 2 cut(s) 1164, 1884
MvnI CGCG 3 cut(s) 210, 303, 826
NarI GGCGCC 1 cut(s) 345
NciI CCSGG 9 cut(s) 635, 1369, 2099, 2459, 2984, 3136, 3231, 3232, 3251
NlaIII CATG 3 cut(s) 2773, 2828, 3358
NmeAIII GCCGAG 3 cut(s) 111, 2841, 3374
NmuCI GTSAC 4 cut(s) 200, 637, 1033, 3148
NspI RCATGY 2 cut(s) 2773, 2828
NspV TTCGAA 2 cut(s) 2544, 2952
PaeR7I CTCGAG 2 cut(s) 1847, 3224
PceI AGGCCT 1 cut(s) 2807
PciSI GCTCTTC 2 cut(s) 1466, 2799
PcsI WCGNNNNNNNCGW 5 cut(s) 185, 791, 2949, 3054, 3401
PdmI GAANNNNTTC 2 cut(s) 545, 1289
PfoI TCCNGGA 1 cut(s) 2097
Ple19I CGATCG 1 cut(s) 3033
PleI GAGTC 3 cut(s) 674, 878, 3380
PluTI GGCGCC 1 cut(s) 348
PpsI GAGTC 3 cut(s) 674, 878, 3380
Ppu21I YACGTR 2 cut(s) 2511, 3049
PpuMI RGGWCCY 1 cut(s) 1195
PshBI ATTAAT 1 cut(s) 1455
Psp124BI GAGCTC 3 cut(s) 246, 909, 1920
Psp1406I AACGTT 1 cut(s) 2931
Psp5II RGGWCCY 1 cut(s) 1195
Psp6I CCWGG 2 cut(s) 1162, 1882
PspEI GGTNACC 1 cut(s) 2986
PspFI CCCAGC 2 cut(s) 171, 3280
PspGI CCWGG 2 cut(s) 1162, 1882
PspPI GGNCC 7 cut(s) 193, 847, 1195, 1298, 2477, 2735, 3377
PspPPI RGGWCCY 1 cut(s) 1195
PspXI VCTCGAGB 2 cut(s) 1847, 3224
PsuI RGATCY 5 cut(s) 111, 1057, 2065, 2104, 2680
PvuI CGATCG 1 cut(s) 3033
PvuII CAGCTG 1 cut(s) 691
RsaI GTAC 8 cut(s) 102, 700, 738, 871, 1804, 2762, 2829, 2907
RsaNI GTAC 8 cut(s) 101, 699, 737, 870, 1803, 2761, 2828, 2906
RseI CAYNNNNRTG 3 cut(s) 509, 2057, 2913
SacI GAGCTC 3 cut(s) 246, 909, 1920
SapI GCTCTTC 2 cut(s) 1466, 2799
Sau96I GGNCC 7 cut(s) 193, 847, 1195, 1298, 2477, 2735, 3377
ScaI AGTACT 1 cut(s) 871
SchI GAGTC 3 cut(s) 674, 878, 3381
SduI GDGCHC 6 cut(s) 246, 909, 1848, 1920, 2619, 3231
SfaNI GCATC 5 cut(s) 786, 939, 1383, 2103, 2707
SfcI CTRYAG 1 cut(s) 325
SfoI GGCGCC 1 cut(s) 346
Sfr274I CTCGAG 2 cut(s) 1847, 3224
SfuI TTCGAA 2 cut(s) 2544, 2952
SgrAI CRCCGGYG 1 cut(s) 427
SinI GGWCC 5 cut(s) 847, 1195, 1298, 2735, 3377
SlaI CTCGAG 2 cut(s) 1847, 3224
SmaI CCCGGG 1 cut(s) 3232
SmiMI CAYNNNNRTG 3 cut(s) 509, 2057, 2913
SmlI CTYRAG 8 cut(s) 653, 662, 1492, 1668, 1847, 2785, 3159, 3224
SmoI CTYRAG 8 cut(s) 653, 662, 1492, 1668, 1847, 2785, 3159, 3224
SpeI ACTAGT 1 cut(s) 1412
SrfI GCCCGGGC 1 cut(s) 3232
SseBI AGGCCT 1 cut(s) 2807
SspDI GGCGCC 1 cut(s) 344
SspMI CTAG 5 cut(s) 1055, 1413, 1925, 2424, 2978
SstI GAGCTC 3 cut(s) 246, 909, 1920
StuI AGGCCT 1 cut(s) 2807
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 6 cut(s) 100, 698, 2059, 2398, 2713, 3206
TatI WGTACW 2 cut(s) 869, 2827
TauI GCSGC 5 cut(s) 77, 213, 273, 1093, 2926
TscAI CASTG 3 cut(s) 634, 2064, 2187
TseFI GTSAC 4 cut(s) 200, 637, 1033, 3148
Tsp45I GTSAC 4 cut(s) 200, 637, 1033, 3148
TspDTI ATGAA 4 cut(s) 1061, 1559, 2667, 3343
TspMI CCCGGG 1 cut(s) 3230
TspRI CASTG 3 cut(s) 634, 2064, 2187
Vha464I CTTAAG 1 cut(s) 2785
VpaK11BI GGWCC 5 cut(s) 847, 1195, 1298, 2735, 3377
VspI ATTAAT 1 cut(s) 1455
XapI RAATTY 5 cut(s) 1158, 1285, 1618, 1878, 2969
XbaI TCTAGA 2 cut(s) 1054, 2977
XceI RCATGY 2 cut(s) 2773, 2828
XcmI CCANNNNNNNNNTGG 1 cut(s) 152
XhoI CTCGAG 2 cut(s) 1847, 3224
XmaI CCCGGG 1 cut(s) 3230
XmnI GAANNNNTTC 2 cut(s) 545, 1289
XspI CTAG 5 cut(s) 1055, 1413, 1925, 2424, 2978
ZraI GACGTC 1 cut(s) 650
ZrmI AGTACT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.