pycom15g01450

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011850
Physical Location & Seq
Forward (+)
837929 .. 841615
3687 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g01450.1

Sequence Viewer

Length: 3561 bp
ATGGCGACGGCCACTTACTTCTTCCTTTTCCTCATACCAATTGTTATTCCTACAATTTATTTCCCCGCCGCCTTAACGGCGGCTCAACGGCCGAGTCCAGAGACGGCACTCTCCGAGATCCAAGCGTTAACGGCGTTTAAGCGTAACCTCCACGACCCTCTTGGCGCTCTCGACGGCTGGGATTCGTCCACGCCGTCAGCCCCGTGCGACTGGCGCGGCGTCGTCTGTTTCAACAACGGAGTTCAGGAACTCCGCCTGCCTCGTCTCCACCTCGGCGGAAGAATCACTGACCACCTCGCCAGCCTCCGCGACCTCCGCAAGCTCAGCCTCTACAGCAACAACTTCAACGGCTCTATACCTCCCTCTCTCTCCCAATGCGCTCTGTTACGGGCCATTTACCTGCATGGCAACTCGCTCACCGGGACTCTTCCGCCGGCGATTTTGAACCTCACCAACCTCCAAATCCTTAATGTCGCCCATAATTTCCTCTCCGGAAAAATATCCGGTGACATTCCGGTGAGCCTCTGGTACCTTGACCTTTCCTCGAACACATTCTCCGGTGAAATTCCGGCGAACTTATCTGCCGAATCAAGCCTCCAGCTGATCAATCTCTCGTTCAATCGGTTCTCCGGCAGGGTTCCGGCGACCATCGGAGGGCTCAAGAATCTTGAGTATCTGTGGCTGGACTCGAACCAGCTGCACGGTACTCTGCCGTCGGCGATTTCCAATTGCTCGTCGCTAGTGCATTTGAGCGCGGACGACAACGAGCTGAGAGGTCTGATTCCGACGACGATCGGAGCGATGCCGAAGCTTCAGGTGTTGTCGCTGTCGCGGAACAACCTGTCAGGGGCGGTCCCCGCGTCGTTGTTGTGCAGTACTGCAAACAACGACTCCTCGTTGAGGATTGTGGAGCTCGGATTCAATGCACTCACGAGCATGGCTCAGCCGCCAGATATCTCAAAATGTTCGAGTGCAATGGGGGTTTTGGACTTAAAATCCAATCGGATAAGTGGCCCCTTCCCATCTTGGTTGACAAACGTGACAACAATTATGAGAATTCTAGATCTTTCTGGAAACATGTTTTCGGGTCAATTGCCGGCGAAGATTGGCAATCTTCTCCAGTTGCAGGAGTTTTGGGCGGCGAATAATTCGCTGAGTGGTGAAATTCCCCGTGAGATTGTGAAATGTAGTTTGTTACAGGTCCTTGATGTTGAAGGAAACAGATTTTTGGGTCAAGTTCCTTCTTTTTTAGGAGGAATGAGGAGCTTGAAGGTGTTGTCTTTGGGTGGGAATTTATTCTCTGGTGCCATTCCGTCAAATTTAGGGGCACTTTCAAAGCTCGAATCTTTGGATTTGAGCAACAATAATCTGACTGGGAAAGTTCCAGATGAGCTCATGATGCAGCTAAGCAACTTGACTGGTTTGAATCTGAGTAATAACAAGTTTTCCGGTGAGATTATTAATGTTGGAGAGCTGAAAAGCTTGCAAGTTTTGAATTTGAGCAATTGTGAGTTTTCTGGAAGTGTTCCTGCGAGCATTGGCGGTTTGATGAAGCTTGAAACACTGGATTTGAGTAAACAGAAGCTTTCTGGGGAGTTGCCGATTCAGATTTTCGGGTTGCCAAATTTACAAGTTGTTGCTGTGCAGGAAAATCGCTTATCAGGAGATGTCCCTCAAGGTTTTAGTAGCTTGATTAGATCGATCTATCTTAACCTATCTTCCAATGCTTTTACTGGTGATATTCCTACCACTTATGGATTTCTCAACTCTTTACTTGTTCTATCTTTGTCTGGAAATCGTATTTCGGGTACAATCCCACAAGAGCTTGGAGATTGTTTGAATCTTCAAGTCCTTGAGCTTCGCTCTAATCAATTAGAAGGCAAAATCCCAGGTGATATATCTCGCCTATCCAATTTGAAAGAACTCAATGTAGGTCAGAATAAGCTGACCGGTGAAATCCCAGAGGAGATTTCAAAATGCTCTTCTCTGACTTCTCTGTTGTTAGATGGGAATCGACTTTCAGGTCACATACCAAACTCATTGCCTAAGTTATTGAACCTCACAGCGTTAGATCTTTCTTCTAACAATCTGAGTGGCACCATCCCTGAAAGTCTGTCATTCCTCGTCCCTAACTTGAAATACCTGAATTTGTCATACAACAACCTTGCTGGAGAGATACCGAAGCCACTCGGTTCTCAGTTCAATGATCCTTCCGTGTTTGCAAGGAATGGAGACTTATGTGGGAAGCCATTGGATAGAGAATGTGCAGATGTGAGAAGGAGAAAAAGAAACAGGTTGACTTTATTAATTGCGGTGGCTGTGGGTGGAGCTTTCCTGCTGGCATTGTGTTGTTGTGGCTATATTTACAGCCTACTGCGATGGCGGAAAAAGCTCAGAGAAACAGTAATTGGGGTGCAGAAGAAGCGAACCAGCCCTCGAACAAGCTCAGGGGATAAAAGCAGCCGGGGAAGTGGCGCTAATGGAGGGCCAAAACTCGTGATGTTCAATAGCAAGATCACGTATGCAGAGGCATTAGAAGCTACAAGACAATTCGATGAGGAAAATGTGCTTAGCAGAGGAAGATATGGATTGGTATTCAAGGCTATGTTTCAAGATGGAATGGTGCTCGCAATTCGACGTCTTACTGATGGTTTTCTTGATGAAGGTGGCTTCCGCAAAGAAGCTGAAGATCTTGGCAAGGTGAAGCATCGAAACCTGACAGTTCTACGAGGCTATTATGCCGGTGCTCCTGACGTGAGGCTCCTTGTGTACGATTACATGCCGAATGGAAACTTAGGCGCTCTTCTACAAGAGGCATCTCACCAAGACGGGCATGTATTAAATTGGCCAATGCGGCACCTCATAGCACTCGGCATTGCTCGTGGATTAGCCTACCTGCACTCTGCCTCAATGGTACACGGCGATGTAAAGCCTCAAAACGTTCTTTTTGATGCTGATTTTGAAGCCCATTTATCCGAATTTGGTCTAGACCGGCTAACCATAGCCACACCAGCAAAAGCTTCCTCGTCTTCAACCACGATTGGCTCCCTAGGCTATGTGTCCCCCGAAGCTGCATTAACAGGGCAAGCAACGAAGGAAGCTGATGTGTATAGTTTTGGGATCGTATTGCTGGAGATATTGACCGGGAGGAATCCGGTGACATTTACTCAAGACGAAGACATTGTGAAGTGGGTGAAGAAGCAACTTCAGAGAGGTCAAGTTTCTGAGCTGCTGGAGCCCGGGCTGCTGGAACTAGACCCGGAATCTTCGGATTGGGAAGAGTTCTTGTTGGGAGTGAAAGTGGGGCTTCTTTGCACGGCTCCTGATCCGCTTGATCGATCATCCATTGCCGACATTGTGTTCATGCTTGAAGGTTGCAGAGTCGGACCCGACATCCCACTACATTATCTTCGTTCCAGCAGTTTTAGGTTTTTGATTAATTCATTTTCTACCATTGGTTTGGCCTTTATTCTTGGGACCACTCCATTATTATTATTTTTTTTTTTAAATTCTATTGAGCTTAGATCCAAACGGTCAGATTTCAAATTTTTAAAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1187

Amino Acids

128.79

Weight (kDa)

6.97

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 38 - 76 1.9e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 177 - 281 3.4e-06 Leucine-rich repeat region
LRR_8 PF13855 198 - 257 2.1e-06 Leucine rich repeat
LRR_14 PF23598 350 - 476 5e-11 Leucine-rich repeat region
LRR_8 PF13855 399 - 457 3.7e-07 Leucine rich repeat
LRR_8 PF13855 421 - 482 8.3e-07 Leucine rich repeat
LRR_14 PF23598 485 - 564 7.3e-07 Leucine-rich repeat region
LRR_14 PF23598 590 - 716 3.1e-06 Leucine-rich repeat region
LRR_8 PF13855 614 - 673 9e-08 Leucine rich repeat
LRR_8 PF13855 662 - 722 7e-06 Leucine rich repeat
Pkinase PF00069 853 - 1070 6.4e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 854 - 1070 2e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75640
fragaria_vesca FvH4_2g39080
malus_domestica MD08G1017600.v1.1 MD08G1017800.v1.1 MD15G1016500.v1.1
prunus_persica Prupe.1G370300_v2.0.a1
pyrus_communis pycom08g01540 pycom15g01450 pycom15g01460
rosa_chinensis RchiOBHm_Chr6g0302191
rosa_laevigata RLG00000011188
rosa_multiflora Rmu_co8058424.1_g000001
rosa_roxburghii Rroxscaffold_7G00166010
rosa_rugosa Rorug06G0313000
rosa_samantha Rh6AG425500 Rh6BG477000 Rh6CG438300 Rh6DG425300
rosa_wichuraiana Rw6G036850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2022
AatII GACGTC 1 cut(s) 2641
Acc36I ACCTGC 2 cut(s) 408, 2904
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 4 cut(s) 528, 1304, 2096, 2856
AccII CGCG 5 cut(s) 216, 309, 755, 832, 860
AccIII TCCGGA 1 cut(s) 491
AclI AACGTT 1 cut(s) 2940
AclWI GGATC 5 cut(s) 112, 2201, 3128, 3320, 3519
AcoI YGGCCR 3 cut(s) 9, 89, 2846
AcuI CTGAAG 3 cut(s) 797, 2706, 3191
AcyI GRCGYC 2 cut(s) 219, 2638
AfaI GTAC 6 cut(s) 530, 706, 877, 1810, 2771, 2916
AfiI CCNNNNNNNGG 4 cut(s) 654, 847, 900, 1126
AflIII ACRYGT 1 cut(s) 1077
AgeI ACCGGT 1 cut(s) 1949
AjiI CACGTC 1 cut(s) 2755
AjnI CCWGG 1 cut(s) 1888
AjuI GAANNNNNNNTTGG 2 cut(s) 2391, 2423
AloI GAACNNNNNNTCC 2 cut(s) 539, 571
Alw21I GWGCWC 4 cut(s) 915, 1395, 2628, 2749
Alw26I GTCTC 3 cut(s) 95, 269, 2226
AlwI GGATC 5 cut(s) 112, 2201, 3128, 3320, 3519
Ama87I CYCGRG 1 cut(s) 3239
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 7 cut(s) 9, 89, 390, 1012, 2486, 2846, 3462
ApeKI GCWGC 6 cut(s) 697, 1402, 2460, 3071, 3229, 3244
AseI ATTAAT 3 cut(s) 1461, 2306, 3438
AsiGI ACCGGT 1 cut(s) 1949
Asp700I GAANNNNTTC 2 cut(s) 551, 1295
Asp718I GGTACC 1 cut(s) 528
AspA2I CCTAGG 1 cut(s) 3049
AspLEI GCGC 6 cut(s) 167, 216, 380, 755, 2477, 2801
AspS9I GGNCC 7 cut(s) 390, 853, 1013, 1201, 2486, 3386, 3477
AsuC2I CCSGG 6 cut(s) 421, 2465, 3145, 3240, 3241, 3260
AvaI CYCGRG 1 cut(s) 3239
AvaII GGWCC 4 cut(s) 853, 1201, 3386, 3477
AvrII CCTAGG 1 cut(s) 3049
BaeGI GKGCMC 1 cut(s) 1330
BalI TGGCCA 1 cut(s) 2848
BanI GGYRCC 4 cut(s) 528, 1304, 2096, 2856
BanII GRGCYC 4 cut(s) 660, 915, 1395, 3240
BauI CACGAG 3 cut(s) 931, 2495, 2880
BbsI GAAGAC 2 cut(s) 3021, 3183
Bbv12I GWGCWC 4 cut(s) 915, 1395, 2628, 2749
BbvI GCAGC 6 cut(s) 684, 1414, 2472, 3058, 3216, 3231
BccI CCATC 7 cut(s) 656, 1030, 2000, 2108, 2373, 2609, 2642
BcgI CGANNNNNNTGC 4 cut(s) 679, 713, 1634, 1668
BciT130I CCWGG 1 cut(s) 1890
BclI TGATCA 1 cut(s) 603
BcnI CCSGG 6 cut(s) 421, 2465, 3145, 3240, 3241, 3260
BcoDI GTCTC 3 cut(s) 95, 269, 2226
BfaI CTAG 5 cut(s) 740, 1061, 2987, 3050, 3254
BfmI CTRYAG 1 cut(s) 331
BfoI RGCGCY 3 cut(s) 168, 2478, 2802
BfuAI ACCTGC 2 cut(s) 408, 2904
BglI GCCNNNNNGGC 2 cut(s) 77, 2854
BglII AGATCT 3 cut(s) 1063, 2071, 2689
BlnI CCTAGG 1 cut(s) 3049
BlpI GCTNAGC 4 cut(s) 323, 942, 1406, 2570
BmcAI AGTACT 1 cut(s) 877
Bme1390I CCNGG 7 cut(s) 421, 1890, 2465, 3145, 3240, 3241, 3260
Bme18I GGWCC 4 cut(s) 853, 1201, 3386, 3477
BmeT110I CYCGRG 1 cut(s) 3239
BmgBI CACGTC 1 cut(s) 2755
BmgT120I GGNCC 7 cut(s) 390, 853, 1013, 1201, 2486, 3386, 3477
BmrFI CCNGG 7 cut(s) 421, 1890, 2465, 3145, 3240, 3241, 3260
BmrI ACTGGG 1 cut(s) 1383
BmsI GCATC 5 cut(s) 792, 1389, 2716, 2825, 2941
BmuI ACTGGG 1 cut(s) 1383
BpiI GAAGAC 2 cut(s) 3021, 3183
BplI GAGNNNNNCTC 4 cut(s) 925, 957, 1847, 1879
BpmI CTGGAG 5 cut(s) 581, 1103, 2190, 3152, 3254
Bpu10I CCTNAGC 1 cut(s) 2446
Bpu1102I GCTNAGC 4 cut(s) 323, 942, 1406, 2570
BpuEI CTTGAG 5 cut(s) 644, 689, 1659, 1874, 3153
BpuMI CCSGG 6 cut(s) 421, 2465, 3145, 3240, 3241, 3260
Bsa29I ATCGAT 2 cut(s) 1700, 3337
BsaAI YACGTR 1 cut(s) 2520
BsaBI GATNNNNATC 1 cut(s) 2010
BsaHI GRCGYC 2 cut(s) 219, 2638
BsaJI CCNNGG 5 cut(s) 271, 1888, 2464, 3049, 3239
BsaWI WCCGGW 7 cut(s) 491, 503, 514, 557, 1448, 1949, 3154
BsaXI ACNNNNNCTCC 6 cut(s) 539, 569, 875, 905, 3285, 3315
Bsc4I CCNNNNNNNGG 4 cut(s) 654, 847, 900, 1126
Bse118I RCCGGY 5 cut(s) 433, 1096, 1949, 2741, 2991
Bse1I ACTGG 6 cut(s) 215, 1120, 1378, 1423, 1569, 1738
Bse3DI GCAATG 4 cut(s) 981, 2039, 2874, 3345
Bse8I GATNNNNATC 1 cut(s) 2010
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 1 cut(s) 1890
BseCI ATCGAT 2 cut(s) 1700, 3337
BseDI CCNNGG 5 cut(s) 271, 1888, 2464, 3049, 3239
BseGI GGATG 3 cut(s) 2100, 3341, 3393
BseJI GATNNNNATC 1 cut(s) 2010
BseLI CCNNNNNNNGG 4 cut(s) 654, 847, 900, 1126
BseMI GCAATG 4 cut(s) 981, 2039, 2874, 3345
BseNI ACTGG 6 cut(s) 215, 1120, 1378, 1423, 1569, 1738
BseRI GAGGAG 3 cut(s) 883, 1276, 1979
BseSI GKGCMC 1 cut(s) 1330
BseX3I CGGCCG 1 cut(s) 89
BseXI GCAGC 6 cut(s) 684, 1414, 2472, 3058, 3216, 3231
BseYI CCCAGC 1 cut(s) 177
BsgI GTGCAG 6 cut(s) 683, 892, 1664, 2286, 2435, 2882
Bsh1236I CGCG 5 cut(s) 216, 309, 755, 832, 860
Bsh1285I CGRYCG 2 cut(s) 92, 795
BshFI GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3464
BshNI GGYRCC 4 cut(s) 528, 1304, 2096, 2856
BshTI ACCGGT 1 cut(s) 1949
BshVI ATCGAT 2 cut(s) 1700, 3337
BsiEI CGRYCG 2 cut(s) 92, 795
BsiHKAI GWGCWC 4 cut(s) 915, 1395, 2628, 2749
BsiHKCI CYCGRG 1 cut(s) 3239
BslFI GGGAC 6 cut(s) 436, 839, 1655, 2111, 3046, 3490
BslI CCNNNNNNNGG 4 cut(s) 654, 847, 900, 1126
BsmAI GTCTC 3 cut(s) 95, 269, 2226
BsmBI CGTCTC 2 cut(s) 95, 269
BsmFI GGGAC 6 cut(s) 436, 839, 1655, 2111, 3046, 3490
BsnI GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3464
BsoBI CYCGRG 1 cut(s) 3239
Bsp1286I GDGCHC 7 cut(s) 660, 915, 1330, 1395, 2628, 2749, 3240
Bsp13I TCCGGA 1 cut(s) 491
Bsp1720I GCTNAGC 4 cut(s) 323, 942, 1406, 2570
BspANI GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3464
BspDI ATCGAT 2 cut(s) 1700, 3337
BspEI TCCGGA 1 cut(s) 491
BspFNI CGCG 5 cut(s) 216, 309, 755, 832, 860
BspHI TCATGA 1 cut(s) 1395
BspMI ACCTGC 2 cut(s) 408, 2904
BspPI GGATC 5 cut(s) 112, 2201, 3128, 3320, 3519
BspQI GCTCTTC 2 cut(s) 1987, 2808
BspT107I GGYRCC 4 cut(s) 528, 1304, 2096, 2856
BsrDI GCAATG 4 cut(s) 981, 2039, 2874, 3345
BsrFI RCCGGY 5 cut(s) 433, 1096, 1949, 2741, 2991
BsrI ACTGG 6 cut(s) 215, 1120, 1378, 1423, 1569, 1738
BssAI RCCGGY 5 cut(s) 433, 1096, 1949, 2741, 2991
BssECI CCNNGG 5 cut(s) 271, 1888, 2464, 3049, 3239
BssNI GRCGYC 2 cut(s) 219, 2638
BssSI CACGAG 3 cut(s) 931, 2495, 2880
BssT1I CCWWGG 1 cut(s) 3049
Bst2BI CACGAG 3 cut(s) 931, 2495, 2880
Bst2UI CCWGG 1 cut(s) 1890
Bst4CI ACNGT 4 cut(s) 704, 2404, 2722, 3534
Bst6I CTCTTC 4 cut(s) 432, 1987, 2808, 3273
BstACI GRCGYC 2 cut(s) 219, 2638
BstBAI YACGTR 1 cut(s) 2520
BstENI CCTNNNNNAGG 1 cut(s) 898
BstF5I GGATG 3 cut(s) 2100, 3341, 3393
BstFNI CGCG 5 cut(s) 216, 309, 755, 832, 860
BstH2I RGCGCY 3 cut(s) 168, 2478, 2802
BstHHI GCGC 6 cut(s) 167, 216, 380, 755, 2477, 2801
BstMAI GTCTC 3 cut(s) 95, 269, 2226
BstMCI CGRYCG 2 cut(s) 92, 795
BstNI CCWGG 1 cut(s) 1890
BstNSI RCATGY 3 cut(s) 1081, 2782, 2837
BstSCI CCNGG 7 cut(s) 419, 1888, 2463, 3143, 3238, 3239, 3258
BstSFI CTRYAG 1 cut(s) 331
BstSLI GKGCMC 1 cut(s) 1330
BstUI CGCG 5 cut(s) 216, 309, 755, 832, 860
BstV1I GCAGC 6 cut(s) 684, 1414, 2472, 3058, 3216, 3231
BstV2I GAAGAC 2 cut(s) 3021, 3183
BstX2I RGATCY 5 cut(s) 117, 1063, 2071, 2689, 3524
BstXI CCANNNNNNTGG 1 cut(s) 3460
BstYI RGATCY 5 cut(s) 117, 1063, 2071, 2689, 3524
BstZI CGGCCG 1 cut(s) 89
Bsu15I ATCGAT 2 cut(s) 1700, 3337
BsuRI GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3464
BsuTUI ATCGAT 2 cut(s) 1700, 3337
BtgZI GCGATG 3 cut(s) 815, 2392, 2937
BtrI CACGTC 1 cut(s) 2755
BtsCI GGATG 3 cut(s) 2100, 3341, 3393
BtsIMutI CAGTG 2 cut(s) 285, 1562
BveI ACCTGC 2 cut(s) 408, 2904
CciI TCATGA 1 cut(s) 1395
CfoI GCGC 6 cut(s) 167, 216, 380, 755, 2477, 2801
Cfr10I RCCGGY 5 cut(s) 433, 1096, 1949, 2741, 2991
Cfr13I GGNCC 7 cut(s) 390, 853, 1013, 1201, 2486, 3386, 3477
Cfr9I CCCGGG 1 cut(s) 3239
ClaI ATCGAT 2 cut(s) 1700, 3337
CseI GACGC 2 cut(s) 208, 849
Csp6I GTAC 6 cut(s) 529, 705, 876, 1809, 2770, 2915
CspAI ACCGGT 1 cut(s) 1949
CspCI CAANNNNNGTGG 2 cut(s) 2074, 2109
CviAII CATG 7 cut(s) 404, 937, 1078, 1396, 2779, 2834, 3364
CviQI GTAC 6 cut(s) 529, 705, 876, 1809, 2770, 2915
DraI TTTAAA 2 cut(s) 3507, 3552
DrdI GACNNNNNNGTC 1 cut(s) 2022
DseDI GACNNNNNNGTC 1 cut(s) 2022
EaeI YGGCCR 3 cut(s) 9, 89, 2846
EagI CGGCCG 1 cut(s) 89
Eam1104I CTCTTC 4 cut(s) 432, 1987, 2808, 3273
EarI CTCTTC 4 cut(s) 432, 1987, 2808, 3273
EciI GGCGGA 4 cut(s) 242, 291, 420, 2398
Ecl136II GAGCTC 2 cut(s) 913, 1393
EclXI CGGCCG 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 3049
Eco24I GRGCYC 4 cut(s) 660, 915, 1395, 3240
Eco32I GATATC 1 cut(s) 955
Eco47I GGWCC 4 cut(s) 853, 1201, 3386, 3477
Eco52I CGGCCG 1 cut(s) 89
Eco53kI GAGCTC 2 cut(s) 913, 1393
Eco57I CTGAAG 3 cut(s) 797, 2706, 3191
Eco88I CYCGRG 1 cut(s) 3239
EcoICRI GAGCTC 2 cut(s) 913, 1393
EcoNI CCTNNNNNAGG 1 cut(s) 898
EcoO109I RGGNCCY 1 cut(s) 1201
EcoRI GAATTC 1 cut(s) 1056
EcoRII CCWGG 1 cut(s) 1888
EcoRV GATATC 1 cut(s) 955
EcoT14I CCWWGG 1 cut(s) 3049
EcoT38I GRGCYC 4 cut(s) 660, 915, 1395, 3240
ErhI CCWWGG 1 cut(s) 3049
Esp3I CGTCTC 2 cut(s) 95, 269
FaeI CATG 7 cut(s) 407, 940, 1081, 1399, 2782, 2837, 3367
FalI AAGNNNNNCTT 2 cut(s) 3291, 3323
FaqI GGGAC 6 cut(s) 436, 839, 1655, 2111, 3046, 3490
FatI CATG 7 cut(s) 403, 936, 1077, 1395, 2778, 2833, 3363
FauI CCCGC 2 cut(s) 73, 865
FbaI TGATCA 1 cut(s) 603
FokI GGATG 3 cut(s) 2087, 3328, 3380
FriOI GRGCYC 4 cut(s) 660, 915, 1395, 3240
FspBI CTAG 5 cut(s) 740, 1061, 2987, 3050, 3254
GlaI GCGC 6 cut(s) 166, 215, 379, 754, 2476, 2800
GsaI CCCAGC 1 cut(s) 181
GsuI CTGGAG 5 cut(s) 581, 1103, 2190, 3152, 3254
HaeII RGCGCY 3 cut(s) 168, 2478, 2802
HaeIII GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3464
HgaI GACGC 2 cut(s) 208, 849
HhaI GCGC 6 cut(s) 167, 216, 380, 755, 2477, 2801
Hin1I GRCGYC 2 cut(s) 219, 2638
Hin1II CATG 7 cut(s) 407, 940, 1081, 1399, 2782, 2837, 3367
Hin6I GCGC 6 cut(s) 165, 214, 378, 753, 2475, 2799
HinP1I GCGC 6 cut(s) 165, 214, 378, 753, 2475, 2799
HincII GTYRAC 3 cut(s) 129, 1032, 2298
HindII GTYRAC 3 cut(s) 129, 1032, 2298
HindIII AAGCTT 5 cut(s) 809, 1480, 1553, 1583, 3018
HpaI GTTAAC 1 cut(s) 129
Hpy166II GTNNAC 7 cut(s) 129, 189, 1032, 1577, 2298, 2770, 2917
Hpy8I GTNNAC 7 cut(s) 129, 189, 1032, 1577, 2298, 2770, 2917
Hpy99I CGWCG 9 cut(s) 10, 176, 224, 718, 739, 790, 793, 865, 2640
HpyCH4III ACNGT 4 cut(s) 704, 2404, 2722, 3534
HpyCH4IV ACGT 5 cut(s) 1038, 2519, 2638, 2754, 2940
HpySE526I ACGT 5 cut(s) 1038, 2519, 2638, 2754, 2940
Hsp92I GRCGYC 2 cut(s) 219, 2638
Hsp92II CATG 7 cut(s) 407, 940, 1081, 1399, 2782, 2837, 3367
HspAI GCGC 6 cut(s) 165, 214, 378, 753, 2475, 2799
Kpn2I TCCGGA 1 cut(s) 491
KpnI GGTACC 1 cut(s) 532
KroI GCCGGC 2 cut(s) 433, 1096
KroNI GCCGGC 2 cut(s) 435, 1098
Ksp22I TGATCA 1 cut(s) 603
KspAI GTTAAC 1 cut(s) 129
LguI GCTCTTC 2 cut(s) 1987, 2808
LmnI GCTCC 9 cut(s) 797, 910, 1263, 2327, 2752, 2766, 3050, 3235, 3325
Lsp1109I GCAGC 6 cut(s) 684, 1414, 2472, 3058, 3216, 3231
LweI GCATC 5 cut(s) 792, 1389, 2716, 2825, 2941
MaeI CTAG 5 cut(s) 740, 1061, 2987, 3050, 3254
MaeII ACGT 5 cut(s) 1038, 2519, 2638, 2754, 2940
MaeIII GTNAC 7 cut(s) 143, 384, 506, 1039, 1194, 2024, 3157
MfeI CAATTG 4 cut(s) 39, 727, 1091, 1504
MflI RGATCY 5 cut(s) 117, 1063, 2071, 2689, 3524
MhlI GDGCHC 7 cut(s) 660, 915, 1330, 1395, 2628, 2749, 3240
MlsI TGGCCA 1 cut(s) 2848
MluNI TGGCCA 1 cut(s) 2848
MlyI GAGTC 5 cut(s) 103, 418, 680, 884, 3390
MmeI TCCRAC 3 cut(s) 809, 1447, 3364
Mox20I TGGCCA 1 cut(s) 2848
MreI CGCCGGCG 1 cut(s) 433
MroI TCCGGA 1 cut(s) 491
MroNI GCCGGC 2 cut(s) 433, 1096
MroXI GAANNNNTTC 2 cut(s) 551, 1295
MscI TGGCCA 1 cut(s) 2848
MslI CAYNNNNRTG 3 cut(s) 515, 935, 2922
Msp20I TGGCCA 1 cut(s) 2848
MspA1I CMGCKG 2 cut(s) 601, 697
MspR9I CCNGG 7 cut(s) 421, 1890, 2465, 3145, 3240, 3241, 3260
MunI CAATTG 4 cut(s) 39, 727, 1091, 1504
MvaI CCWGG 1 cut(s) 1890
MvnI CGCG 5 cut(s) 216, 309, 755, 832, 860
NaeI GCCGGC 2 cut(s) 435, 1098
NciI CCSGG 6 cut(s) 421, 2465, 3145, 3240, 3241, 3260
NgoMIV GCCGGC 2 cut(s) 433, 1096
NlaIII CATG 7 cut(s) 407, 940, 1081, 1399, 2782, 2837, 3367
NmeAIII GCCGAG 3 cut(s) 117, 252, 2850
NmuCI GTSAC 4 cut(s) 506, 1039, 2024, 3157
NspI RCATGY 3 cut(s) 1081, 2782, 2837
PagI TCATGA 1 cut(s) 1395
PciI ACATGT 1 cut(s) 1077
PciSI GCTCTTC 2 cut(s) 1987, 2808
PcsI WCGNNNNNNNCGW 2 cut(s) 191, 797
PdiI GCCGGC 2 cut(s) 435, 1098
PdmI GAANNNNTTC 2 cut(s) 551, 1295
PinAI ACCGGT 1 cut(s) 1949
Ple19I CGATCG 1 cut(s) 795
PleI GAGTC 5 cut(s) 102, 418, 680, 884, 3389
PpsI GAGTC 5 cut(s) 102, 418, 680, 884, 3389
Ppu21I YACGTR 1 cut(s) 2520
PpuMI RGGWCCY 1 cut(s) 1201
PscI ACATGT 1 cut(s) 1077
PshBI ATTAAT 3 cut(s) 1461, 2306, 3438
Psp124BI GAGCTC 2 cut(s) 915, 1395
Psp1406I AACGTT 1 cut(s) 2940
Psp5II RGGWCCY 1 cut(s) 1201
Psp6I CCWGG 1 cut(s) 1888
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 1 cut(s) 1888
PspPI GGNCC 7 cut(s) 390, 853, 1013, 1201, 2486, 3386, 3477
PspPPI RGGWCCY 1 cut(s) 1201
PsuI RGATCY 5 cut(s) 117, 1063, 2071, 2689, 3524
PvuI CGATCG 1 cut(s) 795
PvuII CAGCTG 2 cut(s) 601, 697
RsaI GTAC 6 cut(s) 530, 706, 877, 1810, 2771, 2916
RsaNI GTAC 6 cut(s) 529, 705, 876, 1809, 2770, 2915
RseI CAYNNNNRTG 3 cut(s) 515, 935, 2922
SacI GAGCTC 2 cut(s) 915, 1395
SapI GCTCTTC 2 cut(s) 1987, 2808
Sau96I GGNCC 7 cut(s) 390, 853, 1013, 1201, 2486, 3386, 3477
ScaI AGTACT 1 cut(s) 877
SchI GAGTC 5 cut(s) 103, 418, 680, 884, 3390
ScrFI CCNGG 7 cut(s) 421, 1890, 2465, 3145, 3240, 3241, 3260
SduI GDGCHC 7 cut(s) 660, 915, 1330, 1395, 2628, 2749, 3240
SfaNI GCATC 5 cut(s) 792, 1389, 2716, 2825, 2941
SfcI CTRYAG 1 cut(s) 331
SgrAI CRCCGGYG 1 cut(s) 433
SinI GGWCC 4 cut(s) 853, 1201, 3386, 3477
SmaI CCCGGG 1 cut(s) 3241
SmiMI CAYNNNNRTG 3 cut(s) 515, 935, 2922
SmlI CTYRAG 5 cut(s) 659, 668, 1674, 1853, 3168
SmoI CTYRAG 5 cut(s) 659, 668, 1674, 1853, 3168
SrfI GCCCGGGC 1 cut(s) 3241
SspMI CTAG 5 cut(s) 740, 1061, 2987, 3050, 3254
SstI GAGCTC 2 cut(s) 915, 1395
StyD4I CCNGG 7 cut(s) 419, 1888, 2463, 3143, 3238, 3239, 3258
StyI CCWWGG 1 cut(s) 3049
TaaI ACNGT 4 cut(s) 704, 2404, 2722, 3534
TaiI ACGT 5 cut(s) 1041, 2522, 2641, 2757, 2943
TatI WGTACW 1 cut(s) 875
TauI GCSGC 6 cut(s) 71, 83, 219, 949, 1142, 2857
TscAI CASTG 2 cut(s) 292, 1569
TseFI GTSAC 4 cut(s) 506, 1039, 2024, 3157
TseI GCWGC 6 cut(s) 697, 1402, 2460, 3071, 3229, 3244
Tsp45I GTSAC 4 cut(s) 506, 1039, 2024, 3157
TspDTI ATGAA 4 cut(s) 1565, 2676, 3352, 3432
TspGWI ACGGA 3 cut(s) 252, 1302, 2203
TspMI CCCGGG 1 cut(s) 3239
TspRI CASTG 2 cut(s) 292, 1569
VpaK11BI GGWCC 4 cut(s) 853, 1201, 3386, 3477
VspI ATTAAT 3 cut(s) 1461, 2306, 3438
XagI CCTNNNNNAGG 1 cut(s) 898
XbaI TCTAGA 2 cut(s) 1060, 2986
XceI RCATGY 3 cut(s) 1081, 2782, 2837
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XmaI CCCGGG 1 cut(s) 3239
XmaJI CCTAGG 1 cut(s) 3049
XmnI GAANNNNTTC 2 cut(s) 551, 1295
XspI CTAG 5 cut(s) 740, 1061, 2987, 3050, 3254
ZraI GACGTC 1 cut(s) 2639
ZrmI AGTACT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.