MD08G1017800.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011850
Physical Location & Seq
Reverse (-)
1350078 .. 1353500
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1017800.v1.1.491

Sequence Viewer

Length: 3423 bp
ATGGCAACGGCTAATTTCTCCTTCCTCTTCGTCGTAGCATTTGCGGTTCCTGTTTGCTTCGCCGCCGCCTTAACGGCCGCTCAAAGGCAAACATCACTCTCCGAGATACAAGCGTTAACGGCGTTTAAGCGCAACCTCCACGACCCGCTTGGCGCGCTCGACAGCTGGGATGCGTCCACGCCGTCAGCCCCGTGTGACTGGCGTGGCGTCGGCTGTCACGATAACCGAGTTCGGGAGCTCCGCCTGCCTCGTCTCCACCTCGGCGGCCGAATTACTGACCAGCTCGCCGACCTCCGCGAACTCCAAAAGCTCAGCCTTCACAGCAACAACCTCAACGGCTCCATACCTTCCTCTCTCTCCCAGTGCGCTTTGTTACGCGCTCTTTACTTGCATGGCAACTCGCTCTCCGGGACTCTCCCGACGGCGATTCTCAACCTCAGCAACCTCCAAATCCTCAACGTCGCTCAAAATTTCCTCTCCGGAACAATCTCCGGCAACATTCCGGTAAGCCTTCGGTACCTCGACCTTTCGTCGAACAAATTCTACGGGGAGATTCCGATGAACTTCTCCGCCGACTCTAGCCTCCAGCTCATCAATCTCTCGTTCAATCGGTTCTCCGGCGTGGTTCCGGCGACCATCGCCGATCTCAAGAATCTTGAATATCTGTGGCTGGACTCGAACCAGCTATACGGCACTCTGCCATCGGCGATTTCTAACTGCTCCTTGCTCGTGCATTTGAGCGCGGACGACAACGCGCTGAGAGGTCTGGTTCCGACGACGATCGGAGCGATGCCGAAGCTTCAGGTGGTGTCGCTGTCACGGAACGAACTGTCAGGGTCGGTCCCCACGTCGTTGTTGTGCAATACTGTTAACAACGACTCGTCGTTGAGGATTGTGGAGCTCGGATTCAATGCGCTCACGAGCATGGCTGAGCCGCCAGATATCTCACAATGTTCGAGTGCATTGGGGGTTTTGGACTTAAATTCCAATCAGATAAGTGGCCCCTTCCCATCTTGGTTGACAAACGTGACAACAATAATGAGAGTTCTAAATCTTTCTGGAAACTTGTTTTCGGGTCAATTGCCGCCGGAGATTGGCAATCTTCTCCGGTTGGAGGAGTTTCGGGTGGCGAATAATTCGCTGTCCGGTGAAATTCCTGGTGAGATTGTGAAATGTAGCTTGTTGCAGGTCCTTGATGTTGAAGGAAACAGGTTTTCGGGTCAAGTTCCCTTGTTTTTGGGAGGAATGAGTGGCTTGAAGGTGTTGTCTTTGGGTAGGAATTTATTCTCCAGTGACATTCCGGCGAGTTTAGGAGCGCTTTCGCAGCTCGAATCGTTGAATTTGAGCAACAATAATCTGACTGGGAAAGTTCCTGATGAGCTATTGATGCAGCTAAGCAACTTGACTAGTTTGAATCTGAGTAATAACAAGTTTTCTGGTGAAATGATTAATGTTGGAGAGTTGAAGAGCTTGCAGGTTTTGAATTTGAGCAATTGTGGGTTTTCGGGGAGTATTCCTTCGAGCATTGGGGGTTTGATGAAGCTTGAAACTCTTGATTTGAGTAAACAGAAGCTTTCGGGGGAGTTCCCGGTTCAGATTTTTGGGTTGCCAAACTTACAAGTGGTTGCTGTGCAGGAAAATCACATATCAGGAGATGTCCCTCAAGGTTTTAGTAGTTTGATTAGTTTGGTCTATCTTAATCTATCTTACAATGCTTTTACTGGTGACATTCCTACAACTTACGGATTTCTCAACTCATTAGTTGTTCTATCACTGTCTGGAAATCGCATTTCTGGTACAATCCCACAAGAGCTTGGAGATAGTTTGAATCTTGGAGTGCTCGAGCTTCGTTCTAATCAGTTAGGTGGCGAAATTCCAGGCGGTATCTCTCGCCTATCCAGTTTGAAAGAGCTCAATGTAGGTCATAATAAATTGACAGGTGAAATCCCAAAGGAGATTTCCAATTGCTCTTCTTTGACATCCCTGTTGTTAGATGGCAATCAACTTTCAGGCCAAATACCAGACTCATTGCCTAAGTTATTAAACCTCGCGGTGTTAGATCTTTCCTCTAACAAGCTGAGTGGAGCAATCCCCGAAAGTCTTTCGCTTCTCGTCCCTCACTTGAAATACCTGAATTTGTCAAACAACAACCTTGCTGGAGAGATTCCGAAGACGCTGGGTTCTCAGTTCAATGATTCTTCTGTGTTTGCAATGAATAGAGATTTATGTGGGAAGCCATTGGGTAGAGAATGTGCAGACGTGAGAAGGAGAAAAAGGAATAGGTTGATTTTATTAATTGGGGTGGCCGTGGGAGGAGCTTTGCTTCTGGCATTGTGTTGTTGTGGCTACATTTACAGCATCTTCCGATGGCGAAAAAAGCTTCGGGAAATGGTAACCGGGCCCCAGAAGAAGCGAACAAGCCCTCGAGCAAGCTCAGGAGACAGGAGCCGGGGAAGTGGAGAACATGGAGGGCCAAAACTTGTGATGTTCACTAGCAAGATCACGTATGCAGAGGCATTGGAGGCAACAAGACAATTCGATGAGGAAAATGTGTTGAGCAGGGGAAGATATGGGCTTGTATTCAAGGCTACATTCCAGGATGGAATGGTGCTCTCAATCCGACGCCTTCCCGATGGAATCCTCGATGAGGGCGGTTTTCGCAAAGAAGCTGAAGCTCTCGACAAGGTGAAACATCGAAACCTAACAGTCCTCCGAGGCTATTACGCAGGTCCTCCGGACGTGAGGCTCCTCGTGTACGATTACATGCCAAATGGAAACCTAGGGACTCTGCTCCAAGAGGCTTCTCACCAAGACGGGCACGTACTTAATTGGCCGATGCGGCACCTCATTGTGCTCGGCGTTGCCCGCGGATTAGCATTCCTGCACTCTGTCCCAATAGTACACGGCGATGTGAAGCCGCAGAACGTGCTTTTCGATGCTGATTTCGAAGCACATTTGTCCGAATTTGGACTAGACCGGCTAACCATAGCCACACTGGCAGAAGCAACCTCGTCTACTACTCCGATCGGGTCCTTAGGCTACGTGTCCCCTGAAGCGGCATTGTCGGGGCAAGCGACGAAAGAAGCCGACGTGTATAGCTTCGGGATTGTACTGTTGGAGATACTGACGGGGAGGAAGCCGGTGATGTTTACGGAAGAAGAAGACATTGTGAAATGGGTGAAGAATCAGCTGCAGACGGGTCAAGTTACCGAGCTGCTCGAACCCGGGATGCTCGAGATTGACCCCGAATCGTCGGAGTGGGAAGAGTTCCTGCTCGGAGTGAAAGTGGGGCTTCTTTGTACGGCTCCTGATCCGCTTGATCGTCCATTGATTGCCGACGTTGTGTTCATGCTTGAAGGTTGCAGGGTCGGACAGGATATCCCGTCCTCAGCTGATCCAACCTCTCTGCCTTCGCCGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1141

Amino Acids

123.02

Weight (kDa)

5.61

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 72 4.9e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 170 - 229 1.8e-07 Leucine rich repeat
LRR_8 PF13855 194 - 253 3.6e-06 Leucine rich repeat
LRR_14 PF23598 313 - 472 4.3e-11 Leucine-rich repeat region
LRR_8 PF13855 395 - 453 2.1e-07 Leucine rich repeat
LRR_14 PF23598 481 - 568 1.5e-08 Leucine-rich repeat region
LRR_8 PF13855 537 - 597 2.5e-08 Leucine rich repeat
LRR_14 PF23598 587 - 713 9.6e-06 Leucine-rich repeat region
LRR_8 PF13855 610 - 669 4.8e-08 Leucine rich repeat
Pkinase PF00069 848 - 1065 3.6e-29 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 849 - 1067 1.6e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75640
fragaria_vesca FvH4_2g39080
malus_domestica MD08G1017600.v1.1 MD08G1017800.v1.1 MD15G1016500.v1.1
prunus_persica Prupe.1G370300_v2.0.a1
pyrus_communis pycom08g01540 pycom15g01450 pycom15g01460
rosa_chinensis RchiOBHm_Chr6g0302191
rosa_laevigata RLG00000011188
rosa_multiflora Rmu_co8058424.1_g000001
rosa_roxburghii Rroxscaffold_7G00166010
rosa_rugosa Rorug06G0313000
rosa_samantha Rh6AG425500 Rh6BG477000 Rh6CG438300 Rh6DG425300
rosa_wichuraiana Rw6G036850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1177, 1465, 2717
Acc65I GGTACC 1 cut(s) 516
AccB1I GGYRCC 2 cut(s) 516, 2841
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 3014
AccII CGCG 7 cut(s) 155, 297, 378, 743, 755, 2051, 2868
AccIII TCCGGA 2 cut(s) 479, 2734
AclWI GGATC 2 cut(s) 3305, 3389
AcoI YGGCCR 4 cut(s) 75, 265, 2304, 2831
AcuI CTGAAG 3 cut(s) 785, 2691, 3072
AcyI GRCGYC 2 cut(s) 207, 2623
AfaI GTAC 7 cut(s) 518, 1798, 2756, 2823, 2901, 3111, 3301
AfeI AGCGCT 1 cut(s) 1317
AfiI CCNNNNNNNGG 6 cut(s) 84, 232, 1094, 1114, 2647, 3055
AflIII ACRYGT 2 cut(s) 3042, 3090
AhdI GACNNNNNGTC 1 cut(s) 529
AhlI ACTAGT 1 cut(s) 1406
AjiI CACGTC 4 cut(s) 849, 2260, 2740, 3091
AjnI CCWGG 3 cut(s) 1156, 1876, 2595
AjuI GAANNNNNNNTTGG 2 cut(s) 1942, 1974
Alw21I GWGCWC 6 cut(s) 240, 903, 1842, 1914, 2613, 2856
Alw26I GTCTC 2 cut(s) 257, 2433
AlwI GGATC 2 cut(s) 3305, 3389
Ama87I CYCGRG 4 cut(s) 1841, 2424, 3224, 3233
Aor13HI TCCGGA 2 cut(s) 479, 2734
Aor51HI AGCGCT 1 cut(s) 1317
AoxI GGCC 8 cut(s) 75, 265, 1000, 2011, 2304, 2399, 2471, 2831
ApaI GGGCCC 1 cut(s) 2403
ApeKI GCWGC 4 cut(s) 1324, 1390, 3190, 3214
AseI ATTAAT 2 cut(s) 1449, 2294
Asp700I GAANNNNTTC 2 cut(s) 539, 1283
Asp718I GGTACC 1 cut(s) 516
AspA2I CCTAGG 1 cut(s) 2779
AspLEI GCGC 9 cut(s) 132, 155, 157, 368, 380, 743, 757, 916, 1318
AspS9I GGNCC 8 cut(s) 841, 1001, 1189, 2399, 2400, 2471, 2729, 3030
AsuC2I CCSGG 6 cut(s) 409, 1589, 2398, 2450, 3225, 3226
AsuHPI GGTGA 9 cut(s) 1160, 1172, 1451, 1736, 1952, 2698, 2798, 3154, 3190
AsuII TTCGAA 1 cut(s) 2946
AvaI CYCGRG 4 cut(s) 1841, 2424, 3224, 3233
AvaII GGWCC 4 cut(s) 841, 1189, 2729, 3030
AvrII CCTAGG 1 cut(s) 2779
AxyI CCTNAGG 1 cut(s) 3034
BaeGI GKGCMC 2 cut(s) 2403, 2820
BaeI ACNNNNGTAYC 2 cut(s) 98, 131
BanI GGYRCC 2 cut(s) 516, 2841
BanII GRGCYC 4 cut(s) 240, 903, 1914, 2403
BauI CACGAG 3 cut(s) 728, 919, 2750
BbsI GAAGAC 2 cut(s) 2177, 3168
Bbv12I GWGCWC 6 cut(s) 240, 903, 1842, 1914, 2613, 2856
BbvCI CCTCAGC 2 cut(s) 437, 3388
BbvI GCAGC 4 cut(s) 1336, 1402, 3177, 3201
BccI CCATC 7 cut(s) 644, 709, 1018, 1988, 2361, 2594, 2627
BciT130I CCWGG 3 cut(s) 1158, 1878, 2597
BcnI CCSGG 6 cut(s) 409, 1589, 2398, 2450, 3225, 3226
BcoDI GTCTC 2 cut(s) 257, 2433
BcuI ACTAGT 1 cut(s) 1406
BfaI CTAG 5 cut(s) 579, 1407, 2493, 2780, 2972
BfmI CTRYAG 1 cut(s) 3191
BfoI RGCGCY 1 cut(s) 1319
BfuAI ACCTGC 3 cut(s) 1177, 1465, 2717
BglI GCCNNNNNGGC 3 cut(s) 74, 2839, 2996
BglII AGATCT 1 cut(s) 2059
BlnI CCTAGG 1 cut(s) 2779
BlpI GCTNAGC 3 cut(s) 311, 930, 1394
Bme1390I CCNGG 9 cut(s) 409, 1158, 1589, 1878, 2398, 2450, 2597, 3225, 3226
Bme18I GGWCC 4 cut(s) 841, 1189, 2729, 3030
BmeRI GACNNNNNGTC 1 cut(s) 529
BmeT110I CYCGRG 4 cut(s) 1841, 2424, 3224, 3233
BmgBI CACGTC 4 cut(s) 849, 2260, 2740, 3091
BmgT120I GGNCC 8 cut(s) 841, 1001, 1189, 2399, 2400, 2471, 2729, 3030
BmrFI CCNGG 9 cut(s) 409, 1158, 1589, 1878, 2398, 2450, 2597, 3225, 3226
BmrI ACTGGG 2 cut(s) 355, 1371
BmsI GCATC 7 cut(s) 160, 780, 1377, 2367, 2826, 2926, 3219
BmuI ACTGGG 2 cut(s) 355, 1371
BpiI GAAGAC 2 cut(s) 2177, 3168
BpmI CTGGAG 3 cut(s) 569, 1273, 2178
Bpu10I CCTNAGC 3 cut(s) 437, 2434, 3388
Bpu1102I GCTNAGC 3 cut(s) 311, 930, 1394
Bpu14I TTCGAA 1 cut(s) 2946
BpuEI CTTGAG 2 cut(s) 632, 1647
BpuMI CCSGG 6 cut(s) 409, 1589, 2398, 2450, 3225, 3226
BsaAI YACGTR 3 cut(s) 2505, 2821, 3043
BsaBI GATNNNNATC 1 cut(s) 1998
BsaHI GRCGYC 2 cut(s) 207, 2623
BsaJI CCNNGG 7 cut(s) 259, 2307, 2449, 2713, 2779, 2866, 3224
BsaWI WCCGGW 5 cut(s) 479, 502, 1107, 1145, 2734
BsaXI ACNNNNNCTCC 4 cut(s) 389, 419, 3270, 3300
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 232, 1094, 1114, 2647, 3055
Bse118I RCCGGY 3 cut(s) 2976, 3139, 3415
Bse1I ACTGG 7 cut(s) 203, 361, 1290, 1366, 1726, 1899, 3000
Bse21I CCTNAGG 1 cut(s) 3034
Bse3DI GCAATG 2 cut(s) 2027, 2217
Bse8I GATNNNNATC 1 cut(s) 1998
BseAI TCCGGA 2 cut(s) 479, 2734
BseBI CCWGG 3 cut(s) 1158, 1878, 2597
BseDI CCNNGG 7 cut(s) 259, 2307, 2449, 2713, 2779, 2866, 3224
BseGI GGATG 4 cut(s) 175, 1979, 2605, 3234
BseJI GATNNNNATC 1 cut(s) 1998
BseLI CCNNNNNNNGG 6 cut(s) 84, 232, 1094, 1114, 2647, 3055
BseMI GCAATG 2 cut(s) 2027, 2217
BseMII CTCAG 9 cut(s) 325, 451, 749, 921, 1409, 2069, 2198, 2448, 3402
BseNI ACTGG 7 cut(s) 203, 361, 1290, 1366, 1726, 1899, 3000
BsePI GCGCGC 1 cut(s) 153
BseRI GAGGAG 3 cut(s) 1130, 2328, 2738
BseSI GKGCMC 2 cut(s) 2403, 2820
BseX3I CGGCCG 2 cut(s) 75, 265
BseXI GCAGC 4 cut(s) 1336, 1402, 3177, 3201
BseYI CCCAGC 2 cut(s) 165, 2176
BsgI GTGCAG 3 cut(s) 1652, 2274, 2867
Bsh1236I CGCG 7 cut(s) 155, 297, 378, 743, 755, 2051, 2868
Bsh1285I CGRYCG 4 cut(s) 78, 268, 783, 3027
BshFI GGCC 8 cut(s) 77, 267, 1002, 2013, 2306, 2401, 2473, 2833
BshNI GGYRCC 2 cut(s) 516, 2841
BsiEI CGRYCG 4 cut(s) 78, 268, 783, 3027
BsiHKAI GWGCWC 6 cut(s) 240, 903, 1842, 1914, 2613, 2856
BsiHKCI CYCGRG 4 cut(s) 1841, 2424, 3224, 3233
BslFI GGGAC 7 cut(s) 424, 827, 1643, 2099, 2797, 2876, 3031
BslI CCNNNNNNNGG 6 cut(s) 84, 232, 1094, 1114, 2647, 3055
BsmAI GTCTC 2 cut(s) 257, 2433
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 7 cut(s) 424, 827, 1643, 2099, 2797, 2876, 3031
BsmI GAATGC 1 cut(s) 2876
BsnI GGCC 8 cut(s) 77, 267, 1002, 2013, 2306, 2401, 2473, 2833
BsoBI CYCGRG 4 cut(s) 1841, 2424, 3224, 3233
Bsp119I TTCGAA 1 cut(s) 2946
Bsp120I GGGCCC 1 cut(s) 2399
Bsp1286I GDGCHC 8 cut(s) 240, 903, 1842, 1914, 2403, 2613, 2820, 2856
Bsp13I TCCGGA 2 cut(s) 479, 2734
Bsp143I GATC 8 cut(s) 643, 780, 2059, 2499, 3024, 3310, 3319, 3394
Bsp1720I GCTNAGC 3 cut(s) 311, 930, 1394
BspANI GGCC 8 cut(s) 77, 267, 1002, 2013, 2306, 2401, 2473, 2833
BspCNI CTCAG 9 cut(s) 324, 450, 750, 922, 1410, 2070, 2197, 2447, 3401
BspEI TCCGGA 2 cut(s) 479, 2734
BspFNI CGCG 7 cut(s) 155, 297, 378, 743, 755, 2051, 2868
BspMAI CTGCAG 1 cut(s) 3195
BspMI ACCTGC 3 cut(s) 1177, 1465, 2717
BspPI GGATC 2 cut(s) 3305, 3389
BspQI GCTCTTC 2 cut(s) 1460, 1975
BspT104I TTCGAA 1 cut(s) 2946
BspT107I GGYRCC 2 cut(s) 516, 2841
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 2 cut(s) 2027, 2217
BsrFI RCCGGY 3 cut(s) 2976, 3139, 3415
BsrI ACTGG 7 cut(s) 203, 361, 1290, 1366, 1726, 1899, 3000
BssAI RCCGGY 3 cut(s) 2976, 3139, 3415
BssECI CCNNGG 7 cut(s) 259, 2307, 2449, 2713, 2779, 2866, 3224
BssHII GCGCGC 1 cut(s) 153
BssMI GATC 8 cut(s) 643, 780, 2059, 2499, 3024, 3310, 3319, 3394
BssNI GRCGYC 2 cut(s) 207, 2623
BssSI CACGAG 3 cut(s) 728, 919, 2750
BssT1I CCWWGG 1 cut(s) 2779
Bst2BI CACGAG 3 cut(s) 728, 919, 2750
Bst2UI CCWGG 3 cut(s) 1158, 1878, 2597
Bst4CI ACNGT 5 cut(s) 831, 868, 1776, 2707, 3114
Bst6I CTCTTC 4 cut(s) 32, 1460, 1975, 3258
BstACI GRCGYC 2 cut(s) 207, 2623
BstAPI GCANNNNNTGC 1 cut(s) 2926
BstBAI YACGTR 3 cut(s) 2505, 2821, 3043
BstBI TTCGAA 1 cut(s) 2946
BstC8I GCNNGC 7 cut(s) 155, 245, 285, 1472, 2431, 2866, 3072
BstDSI CCRYGG 2 cut(s) 2307, 2866
BstEII GGTNACC 1 cut(s) 2392
BstENI CCTNNNNNAGG 1 cut(s) 2645
BstF5I GGATG 4 cut(s) 175, 1979, 2605, 3234
BstFNI CGCG 7 cut(s) 155, 297, 378, 743, 755, 2051, 2868
BstH2I RGCGCY 1 cut(s) 1319
BstHHI GCGC 9 cut(s) 132, 155, 157, 368, 380, 743, 757, 916, 1318
BstKTI GATC 8 cut(s) 646, 783, 2062, 2502, 3027, 3313, 3322, 3397
BstMAI GTCTC 2 cut(s) 257, 2433
BstMBI GATC 8 cut(s) 643, 780, 2059, 2499, 3024, 3310, 3319, 3394
BstMCI CGRYCG 4 cut(s) 78, 268, 783, 3027
BstNI CCWGG 3 cut(s) 1158, 1878, 2597
BstNSI RCATGY 1 cut(s) 2767
BstPI GGTNACC 1 cut(s) 2392
BstSCI CCNGG 9 cut(s) 407, 1156, 1587, 1876, 2396, 2448, 2595, 3223, 3224
BstSFI CTRYAG 1 cut(s) 3191
BstSLI GKGCMC 2 cut(s) 2403, 2820
BstUI CGCG 7 cut(s) 155, 297, 378, 743, 755, 2051, 2868
BstV1I GCAGC 4 cut(s) 1336, 1402, 3177, 3201
BstV2I GAAGAC 2 cut(s) 2177, 3168
BstX2I RGATCY 1 cut(s) 2059
BstYI RGATCY 1 cut(s) 2059
BstZI CGGCCG 2 cut(s) 75, 265
Bsu36I CCTNAGG 1 cut(s) 3034
BsuRI GGCC 8 cut(s) 77, 267, 1002, 2013, 2306, 2401, 2473, 2833
BtgI CCRYGG 2 cut(s) 2307, 2866
BtgZI GCGATG 3 cut(s) 622, 803, 2922
BtrI CACGTC 4 cut(s) 849, 2260, 2740, 3091
BtsCI GGATG 4 cut(s) 175, 1979, 2605, 3234
BtsIMutI CAGTG 4 cut(s) 368, 1297, 1772, 2993
BveI ACCTGC 3 cut(s) 1177, 1465, 2717
Cac8I GCNNGC 7 cut(s) 155, 245, 285, 1472, 2431, 2866, 3072
CfoI GCGC 9 cut(s) 132, 155, 157, 368, 380, 743, 757, 916, 1318
Cfr10I RCCGGY 3 cut(s) 2976, 3139, 3415
Cfr13I GGNCC 8 cut(s) 841, 1001, 1189, 2399, 2400, 2471, 2729, 3030
Cfr42I CCGCGG 1 cut(s) 2869
Cfr9I CCCGGG 1 cut(s) 3224
CseI GACGC 4 cut(s) 162, 196, 2182, 2631
Csp6I GTAC 7 cut(s) 517, 1797, 2755, 2822, 2900, 3110, 3300
CspCI CAANNNNNGTGG 2 cut(s) 2062, 2097
CviAII CATG 5 cut(s) 392, 925, 2465, 2764, 3349
CviQI GTAC 7 cut(s) 517, 1797, 2755, 2822, 2900, 3110, 3300
DpnI GATC 8 cut(s) 645, 782, 2061, 2501, 3026, 3312, 3321, 3396
DpnII GATC 8 cut(s) 643, 780, 2059, 2499, 3024, 3310, 3319, 3394
DriI GACNNNNNGTC 1 cut(s) 529
EaeI YGGCCR 4 cut(s) 75, 265, 2304, 2831
EagI CGGCCG 2 cut(s) 75, 265
Eam1104I CTCTTC 4 cut(s) 32, 1460, 1975, 3258
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 4 cut(s) 32, 1460, 1975, 3258
EciI GGCGGA 2 cut(s) 230, 559
Ecl136II GAGCTC 3 cut(s) 238, 901, 1912
EclXI CGGCCG 2 cut(s) 75, 265
Eco130I CCWWGG 1 cut(s) 2779
Eco24I GRGCYC 4 cut(s) 240, 903, 1914, 2403
Eco32I GATATC 2 cut(s) 943, 3379
Eco47I GGWCC 4 cut(s) 841, 1189, 2729, 3030
Eco47III AGCGCT 1 cut(s) 1317
Eco52I CGGCCG 2 cut(s) 75, 265
Eco53kI GAGCTC 3 cut(s) 238, 901, 1912
Eco57I CTGAAG 3 cut(s) 785, 2691, 3072
Eco81I CCTNAGG 1 cut(s) 3034
Eco88I CYCGRG 4 cut(s) 1841, 2424, 3224, 3233
Eco91I GGTNACC 1 cut(s) 2392
EcoICRI GAGCTC 3 cut(s) 238, 901, 1912
EcoNI CCTNNNNNAGG 1 cut(s) 2645
EcoO109I RGGNCCY 4 cut(s) 1189, 2400, 2729, 3030
EcoO65I GGTNACC 1 cut(s) 2392
EcoRII CCWGG 3 cut(s) 1156, 1876, 2595
EcoRV GATATC 2 cut(s) 943, 3379
EcoT14I CCWWGG 1 cut(s) 2779
EcoT38I GRGCYC 4 cut(s) 240, 903, 1914, 2403
ErhI CCWWGG 1 cut(s) 2779
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 5 cut(s) 395, 928, 2468, 2767, 3352
FalI AAGNNNNNCTT 2 cut(s) 3276, 3308
FaqI GGGAC 7 cut(s) 424, 827, 1643, 2099, 2797, 2876, 3031
FatI CATG 5 cut(s) 391, 924, 2464, 2763, 3348
FauI CCCGC 2 cut(s) 153, 2873
FblI GTMKAC 1 cut(s) 3014
FokI GGATG 4 cut(s) 182, 1966, 2612, 3241
FriOI GRGCYC 4 cut(s) 240, 903, 1914, 2403
FspBI CTAG 5 cut(s) 579, 1407, 2493, 2780, 2972
GlaI GCGC 9 cut(s) 131, 154, 156, 367, 379, 742, 756, 915, 1317
GsaI CCCAGC 2 cut(s) 169, 2180
GsuI CTGGAG 3 cut(s) 569, 1273, 2178
HaeII RGCGCY 1 cut(s) 1319
HaeIII GGCC 8 cut(s) 77, 267, 1002, 2013, 2306, 2401, 2473, 2833
HgaI GACGC 4 cut(s) 162, 196, 2182, 2631
HhaI GCGC 9 cut(s) 132, 155, 157, 368, 380, 743, 757, 916, 1318
Hin1I GRCGYC 2 cut(s) 207, 2623
Hin1II CATG 5 cut(s) 395, 928, 2468, 2767, 3352
Hin6I GCGC 9 cut(s) 130, 153, 155, 366, 378, 741, 755, 914, 1316
HinP1I GCGC 9 cut(s) 130, 153, 155, 366, 378, 741, 755, 914, 1316
HincII GTYRAC 3 cut(s) 117, 871, 1020
HindII GTYRAC 3 cut(s) 117, 871, 1020
HindIII AAGCTT 4 cut(s) 797, 1541, 1571, 2378
HpaI GTTAAC 2 cut(s) 117, 871
HphI GGTGA 9 cut(s) 1160, 1172, 1451, 1736, 1952, 2698, 2798, 3154, 3190
HpyCH4III ACNGT 5 cut(s) 831, 868, 1776, 2707, 3114
Hsp92I GRCGYC 2 cut(s) 207, 2623
Hsp92II CATG 5 cut(s) 395, 928, 2468, 2767, 3352
HspAI GCGC 9 cut(s) 130, 153, 155, 366, 378, 741, 755, 914, 1316
Kpn2I TCCGGA 2 cut(s) 479, 2734
KpnI GGTACC 1 cut(s) 520
KspAI GTTAAC 2 cut(s) 117, 871
KspI CCGCGG 1 cut(s) 2869
Kzo9I GATC 8 cut(s) 643, 780, 2059, 2499, 3024, 3310, 3319, 3394
LguI GCTCTTC 2 cut(s) 1460, 1975
Lsp1109I GCAGC 4 cut(s) 1336, 1402, 3177, 3201
LweI GCATC 7 cut(s) 160, 780, 1377, 2367, 2826, 2926, 3219
MaeI CTAG 5 cut(s) 579, 1407, 2493, 2780, 2972
MaeIII GTNAC 9 cut(s) 194, 215, 372, 816, 1027, 1292, 1724, 2392, 3205
MalI GATC 8 cut(s) 645, 782, 2061, 2501, 3026, 3312, 3321, 3396
MbiI CCGCTC 1 cut(s) 80
MboI GATC 8 cut(s) 643, 780, 2059, 2499, 3024, 3310, 3319, 3394
MfeI CAATTG 3 cut(s) 1079, 1492, 1963
MflI RGATCY 1 cut(s) 2059
MhlI GDGCHC 8 cut(s) 240, 903, 1842, 1914, 2403, 2613, 2820, 2856
MlyI GAGTC 6 cut(s) 406, 569, 668, 872, 2018, 2779
MmeI TCCRAC 8 cut(s) 797, 1092, 1435, 2644, 3096, 3234, 3349, 3422
MroI TCCGGA 2 cut(s) 479, 2734
MroXI GAANNNNTTC 2 cut(s) 539, 1283
MslI CAYNNNNRTG 2 cut(s) 923, 2907
MspA1I CMGCKG 4 cut(s) 165, 2868, 3190, 3392
MspR9I CCNGG 9 cut(s) 409, 1158, 1589, 1878, 2398, 2450, 2597, 3225, 3226
MunI CAATTG 3 cut(s) 1079, 1492, 1963
Mva1269I GAATGC 1 cut(s) 2876
MvaI CCWGG 3 cut(s) 1158, 1878, 2597
MvnI CGCG 7 cut(s) 155, 297, 378, 743, 755, 2051, 2868
NciI CCSGG 6 cut(s) 409, 1589, 2398, 2450, 3225, 3226
NdeII GATC 8 cut(s) 643, 780, 2059, 2499, 3024, 3310, 3319, 3394
NlaIII CATG 5 cut(s) 395, 928, 2468, 2767, 3352
NmeAIII GCCGAG 2 cut(s) 240, 2835
NmuCI GTSAC 6 cut(s) 194, 215, 816, 1027, 1292, 1724
NspI RCATGY 1 cut(s) 2767
NspV TTCGAA 1 cut(s) 2946
PaeR7I CTCGAG 3 cut(s) 1841, 2424, 3233
PauI GCGCGC 1 cut(s) 153
PciSI GCTCTTC 2 cut(s) 1460, 1975
PcsI WCGNNNNNNNCGW 5 cut(s) 216, 785, 845, 2931, 3071
PctI GAATGC 1 cut(s) 2876
PdmI GAANNNNTTC 2 cut(s) 539, 1283
PfoI TCCNGGA 2 cut(s) 407, 2595
Ple19I CGATCG 2 cut(s) 783, 3027
PleI GAGTC 6 cut(s) 406, 569, 668, 872, 2018, 2779
PpsI GAGTC 6 cut(s) 406, 569, 668, 872, 2018, 2779
Ppu21I YACGTR 3 cut(s) 2505, 2821, 3043
PpuMI RGGWCCY 3 cut(s) 1189, 2729, 3030
PshBI ATTAAT 2 cut(s) 1449, 2294
Psp124BI GAGCTC 3 cut(s) 240, 903, 1914
Psp5II RGGWCCY 3 cut(s) 1189, 2729, 3030
Psp6I CCWGG 3 cut(s) 1156, 1876, 2595
PspEI GGTNACC 1 cut(s) 2392
PspFI CCCAGC 2 cut(s) 165, 2176
PspGI CCWGG 3 cut(s) 1156, 1876, 2595
PspOMI GGGCCC 1 cut(s) 2399
PspPI GGNCC 8 cut(s) 841, 1001, 1189, 2399, 2400, 2471, 2729, 3030
PspPPI RGGWCCY 3 cut(s) 1189, 2729, 3030
PspXI VCTCGAGB 2 cut(s) 1841, 2424
PsrI GAACNNNNNNTAC 4 cut(s) 527, 559, 671, 703
PstI CTGCAG 1 cut(s) 3195
PsuI RGATCY 1 cut(s) 2059
PteI GCGCGC 1 cut(s) 153
PvuI CGATCG 2 cut(s) 783, 3027
PvuII CAGCTG 3 cut(s) 165, 3190, 3392
RsaI GTAC 7 cut(s) 518, 1798, 2756, 2823, 2901, 3111, 3301
RsaNI GTAC 7 cut(s) 517, 1797, 2755, 2822, 2900, 3110, 3300
RseI CAYNNNNRTG 2 cut(s) 923, 2907
SacI GAGCTC 3 cut(s) 240, 903, 1914
SacII CCGCGG 1 cut(s) 2869
SapI GCTCTTC 2 cut(s) 1460, 1975
Sau3AI GATC 8 cut(s) 643, 780, 2059, 2499, 3024, 3310, 3319, 3394
Sau96I GGNCC 8 cut(s) 841, 1001, 1189, 2399, 2400, 2471, 2729, 3030
SchI GAGTC 6 cut(s) 406, 569, 668, 872, 2018, 2779
ScrFI CCNGG 9 cut(s) 409, 1158, 1589, 1878, 2398, 2450, 2597, 3225, 3226
SduI GDGCHC 8 cut(s) 240, 903, 1842, 1914, 2403, 2613, 2820, 2856
SfaNI GCATC 7 cut(s) 160, 780, 1377, 2367, 2826, 2926, 3219
SfcI CTRYAG 1 cut(s) 3191
Sfr274I CTCGAG 3 cut(s) 1841, 2424, 3233
Sfr303I CCGCGG 1 cut(s) 2869
SfuI TTCGAA 1 cut(s) 2946
SgrBI CCGCGG 1 cut(s) 2869
SinI GGWCC 4 cut(s) 841, 1189, 2729, 3030
SlaI CTCGAG 3 cut(s) 1841, 2424, 3233
SmaI CCCGGG 1 cut(s) 3226
SmiMI CAYNNNNRTG 2 cut(s) 923, 2907
SmlI CTYRAG 5 cut(s) 647, 1662, 1841, 2424, 3233
SmoI CTYRAG 5 cut(s) 647, 1662, 1841, 2424, 3233
SpeI ACTAGT 1 cut(s) 1406
SspMI CTAG 5 cut(s) 579, 1407, 2493, 2780, 2972
SstI GAGCTC 3 cut(s) 240, 903, 1914
StyD4I CCNGG 9 cut(s) 407, 1156, 1587, 1876, 2396, 2448, 2595, 3223, 3224
StyI CCWWGG 1 cut(s) 2779
TaaI ACNGT 5 cut(s) 831, 868, 1776, 2707, 3114
TaqII GACCGA 1 cut(s) 829
TatI WGTACW 2 cut(s) 2899, 3109
TauI GCSGC 9 cut(s) 65, 68, 80, 267, 937, 1087, 2842, 2920, 3059
TscAI CASTG 4 cut(s) 368, 1297, 1779, 3000
TseFI GTSAC 6 cut(s) 194, 215, 816, 1027, 1292, 1724
TseI GCWGC 4 cut(s) 1324, 1390, 3190, 3214
Tsp45I GTSAC 6 cut(s) 194, 215, 816, 1027, 1292, 1724
TspDTI ATGAA 4 cut(s) 575, 1553, 2228, 3337
TspGWI ACGGA 3 cut(s) 835, 1758, 3167
TspMI CCCGGG 1 cut(s) 3224
TspRI CASTG 4 cut(s) 368, 1297, 1779, 3000
VpaK11BI GGWCC 4 cut(s) 841, 1189, 2729, 3030
VspI ATTAAT 2 cut(s) 1449, 2294
XagI CCTNNNNNAGG 1 cut(s) 2645
XceI RCATGY 1 cut(s) 2767
XcmI CCANNNNNNNNNTGG 2 cut(s) 146, 2992
XhoI CTCGAG 3 cut(s) 1841, 2424, 3233
XmaI CCCGGG 1 cut(s) 3224
XmaJI CCTAGG 1 cut(s) 2779
XmiI GTMKAC 1 cut(s) 3014
XmnI GAANNNNTTC 2 cut(s) 539, 1283
XspI CTAG 5 cut(s) 579, 1407, 2493, 2780, 2972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.