pycom15g01460

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011850
Physical Location & Seq
Reverse (-)
845660 .. 849601
3942 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g01460.1

Sequence Viewer

Length: 3726 bp
ATGCCTGTTTCTTTTGGGGAAACGGGAAAAACAAAAAATAAAAAATCCAGAGCGACAGTGGTAGGGAACACAAACGCAGACGCACAAATTAGTTGCCCTCCAAGCGCAGCATATGGCGTCGTTTCTCTCAAAACCCCTTTCCCTTCCGTTTCTTCACACGCTCCTCCCACCCCACCACAAACCCAGCACTCCTCCCACTGCCACCAAAAATACCCAACAATTAACATAAAGTTGGAATTGTATACCCAGCACATTCGGCGTTCCACTACTCTCTCTGACCTCACCGCATACCCAGCACTACTCATGGCAACGGCTACTTTCTACTTCCTCTTCATCGTAGCATTTGCTGTTCCTATTTGCTTCGCCGCCACTTTAACGCCCGCTCAACGGCAAGCATCATTACCGGAGATCCAAGCGTTAATGGCGTTTAAGCGCAACCTCTACGACCCGCTTGGCGCGCTGGACGGCTGGGATGCTTCCACGCCGTCAGCCCCGTGCGACTGGCGTGGCGTTGGCTGTGACGATAACCGAGTTCAGGAGCTCCGCCTGCCTCGTCTCCACCTCGGCGGCCGAATTACTGACCACCTCGCCGACCTACGCGACCTCCGCAAGCTCAGCCTCCACAGCAACAACCTCAACGGCTCTATACCTTCCTCTCTCTCCCAATGCGCCTTGTTACGAGTTCTTTACTTGCACGGCAACTCCCTCTCCGGGATTCTCCCGCCGGCGATTTTCAACCTCACCAACCTCCAAATCCTCAACGTCGCTCACAACTTCCTCTCTGGAAAAATCTCCGGCGACATTCCGGTGAGCCTTCGGTTCCTCGACCTTTCATCGAACACATTCGCCGGGGAGATTCCGGCGAATTTCTCCGCCGACTCAAGCCTCCAGCTCGTCAACCTCTCGTTCAATCGGTTCTCCGGCGTGGTTCCGGCAACCATCGGAGGTCTCAAGAATCTTGAATATCTGTGGTTTGACTCGAACCAGCTGTATGGCACTCTGCCCTCAGCGATTTCCAACTGCTCGTCGCTCGTACATTTGAGCGCCGACGACAACTCGCTGAGAGGTCTGGTTCCGACGACGATCGGAGCGATGCCGAAGCTTCAGGTGGTGTCGCTGTCACGGAATGAGCTGTCAGGGTCTGTCCCCACGTCGTTGTTGTGCAATACTGCTAACAACGACTCGTCGATGACGATCGTGGAGCTCGGATTCAATGCGCTCACGAGCATTGCTGAGCCGTCAGATATCTCAAAATGTTCGAGTGTATTGGGGGTTCTGGACTTAAAATCCAATCGGATAAGTGGACCGTTCCCTTCTTGGTTGACAAACGTGACAACAATTATGAGAGTTCTCGATCTTTCTGGAAACATGTTATCGGGTCAACTACCGGCGGAGATTGGCAATCTTCTCCGGTTGGAGGAGTTTCGGGTGGCAAATAATTCGCTGACTGGTGAACTTCCCAGTGAGATTGTGAAATGTAATTTGTTACAGGTCCTTGATGTTGAAGGAAACAGATTTTTGGGTCAAGTTCCTTTGTTTTTGGGAGGAATGAGTAGCTTGAAGGTGTTGTCTTTGGGTAGGAATTTATTCTCCGGCGACATTCCGGCAAGTTTAGGAGCGCTTTCGCAGCTCGAATCCTTGAATTTGAGCAACAATAATCTGACCGGGAAAGTTCCAGATGAGCTCATGATGCAGCTAAGCAACTTGACCAGTTTGAATCTGAGTAACAACAGATTTTCTGGTGAGATTATTAATGTTGGAGATTTGAAGAGCTTGCAGGTTTTGAATTTGAGCAATTGCGGGTTTTCGGGGAGTGTTCCTGCGAGCATTGGTGGTTTGATGAAGCTTGAAACACTGGATTTGAGTAAGCAGAAGCTTTCCGGGGAGTTGCCGGTTCAGATTTTCGGGTTGCCAAATTTACAAGTGGTTGCTCTGCAGGAAAATCACTTATCTGGAGATGTCCCTCAAGGTTTTAGTAGCTTGATTAGTTTGATCTATCTAAACATGTCTTCCAATGCCTTTACTGGTGTTATTCCCACCACTTACGGATTTCTCAACTCATTACTTGTTCTATCATTGTCTGGAAATCGCATTTCGGGCACAATCCCACAAGAGCTTGGAGATGGTTTGAATCTTCGAGTGCTCGAGCTTCGTTCTAATCAATTAGGAGGCGAAATTCCAGCCGATATCTCTCGCCTCTCCAATTTGAAAGAGCTCAATGTAGGTCACAATAAGTTGACAGGTGAAATCCCAGAGGAGATTTCCAACTGCTCCTCCCTGACTTCTCTGTTGTTGGATGGCAATCAACTTTCGGGTCAAATACCAAACTCATTGCCTAAGTTATTGAACCTCACAGTGTTAGATCTTTCCTCTAACAGGCTGAGCGGCGCCATCCCAGATAATCTTTCGCTCCTCGTCCCTCACTTGAAATACCTGAATTTGTCAAACAACAACCTTGCTGGAGAGATTCCAAAGCCGCTGGGTTCCCAGTTCAACGATCCTTCTGTGTTTGCAATGAACAGAGATTTATGTGGGAAGCCGTTGAGTAGAGAATGTGCAGTTGTGAGAAGGAGAAAAAGGAATAGGTTGATTTTATTAGTTGGGGTGGCTGTGGCAGGAGCTTTGCTTCTGGCATTGTGTTGTTGTGGCTACGTTTACAGCCTCTTGCGATGGCGGAAAAAGCTTAGAGAAATGGTAACGGGGCCCCAAAAGAAGCGAACAAGCCCACGAGGAAGCTCAGGAGACAGGAGCCGGGGAAGTGGAGAAAATGGAGGGCCAAAACTGGTAATGTTCACCAGCAAGATCACGTATGCGGAGGCATTAGAAGCAACAAGACAATTTGAGGAGGAAAATGTGCTAAGCAGGGGAAGATATGGACCTGTATTCAAGGCTACATTCCAAGATGGAATGGTGCTCTCAATTCGGCGTCTTCCCGATGGATTCCTCGATGAAGGTGGTTTTCGCAAAGAAGCTGAAGCTCTCGGCAAGGTGAAGCATCGAAACCTAACAGTCCTCCGAGGCTACTACGCAGGTCCTCCCGACGTGAGGCTCCTCGTGTACGATTACATGCCAAATGGAAACTTAGGCACTCTTCTCCAAGAGGCATCTCACCAAGACGGGCACGTACTAAATTGGCCAATGCGGCACCTCATTGCGCTCGGCATTGCCCGCGGATTAGCATTCCTCCACTCCATGTTAATCGTACACGGCGACGTAAAGCCTCAAAACGTTCTTTTCGATGCTGATTTCGAAGCGCATTTGTCCGAATTCGGACTAGACCGGCTAACCATAGCCACACCAGCAGAAGCTTCATCGTCTACTACGCCGATCGGGTCCTTAGGCTACGTGTCCCCTGAAGCTGCATTGACGGGGCAAGCGACGAAAGAAGCCGACGTGTATAGTTTCGGGATTGTATTGTTGGAGATTTTAACCGGGAGGAAGCCGGTGATGTTTACACAAGACGAAGACATTGTCAAGTGGGTGAAGAAGCAGCTGCAGAGTGGTCAAGTTTCCGAGCTGCTCGAGCCCGGGCTGCTCGAAATTGACCCCGAATCGTCGGATTGGGAAGAGTTCTTGCTCGGAGTGAAAGTGGGGCTTCTTTGCACGGCTCCTGATCCGCTTGATCGGCCGCTGATTGCCGACATTGTGTTCATGCTTGAAGGTTGCAGGGTCGCACAGGATATTCCGTCCTCAGCTGATCCCACCTCTCTGCCTTCACCGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1242

Amino Acids

133.97

Weight (kDa)

6.35

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 135 - 174 3e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 295 - 354 1.9e-06 Leucine rich repeat
LRR_14 PF23598 420 - 573 7.1e-13 Leucine-rich repeat region
LRR_8 PF13855 496 - 554 2.8e-07 Leucine rich repeat
LRR_8 PF13855 519 - 579 3.4e-07 Leucine rich repeat
LRR_14 PF23598 584 - 670 3.3e-08 Leucine-rich repeat region
LRR_8 PF13855 615 - 673 6e-06 Leucine rich repeat
LRR_8 PF13855 638 - 698 4.4e-08 Leucine rich repeat
LRR_14 PF23598 688 - 792 3.1e-06 Leucine-rich repeat region
LRR_8 PF13855 711 - 770 8.1e-08 Leucine rich repeat
Pkinase PF00069 947 - 1165 1.4e-29 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 950 - 1167 6.7e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75640
fragaria_vesca FvH4_2g39080
malus_domestica MD08G1017600.v1.1 MD08G1017800.v1.1 MD15G1016500.v1.1
prunus_persica Prupe.1G370300_v2.0.a1
pyrus_communis pycom08g01540 pycom15g01450 pycom15g01460
rosa_chinensis RchiOBHm_Chr6g0302191
rosa_laevigata RLG00000011188
rosa_multiflora Rmu_co8058424.1_g000001
rosa_roxburghii Rroxscaffold_7G00166010
rosa_rugosa Rorug06G0313000
rosa_samantha Rh6AG425500 Rh6BG477000 Rh6CG438300 Rh6DG425300
rosa_wichuraiana Rw6G036850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1768, 3020
AccB1I GGYRCC 2 cut(s) 2387, 3144
AccBSI CCGCTC 2 cut(s) 383, 2385
AccI GTMKAC 2 cut(s) 242, 3317
AccII CGCG 3 cut(s) 458, 600, 3171
AclI AACGTT 1 cut(s) 3228
AclWI GGATC 4 cut(s) 403, 2492, 3608, 3692
AcoI YGGCCR 3 cut(s) 568, 3134, 3626
AcsI RAATTY 8 cut(s) 865, 1582, 1642, 1786, 1915, 2175, 2437, 3266
AcuI CTGAAG 3 cut(s) 1088, 2994, 3375
AcyI GRCGYC 3 cut(s) 117, 2388, 2926
AfaI GTAC 4 cut(s) 1035, 3059, 3126, 3204
AfeI AGCGCT 1 cut(s) 1620
AfiI CCNNNNNNNGG 6 cut(s) 387, 535, 711, 1417, 2376, 3045
AflIII ACRYGT 4 cut(s) 1368, 2004, 3345, 3393
AgeI ACCGGT 1 cut(s) 3718
AjiI CACGTC 3 cut(s) 1152, 3043, 3394
Alw21I GWGCWC 6 cut(s) 543, 1206, 1686, 2145, 2217, 2916
Alw26I GTCTC 3 cut(s) 560, 953, 2736
AlwI GGATC 4 cut(s) 403, 2492, 3608, 3692
AlwNI CAGNNNCTG 1 cut(s) 1142
Ama87I CYCGRG 3 cut(s) 2144, 3521, 3527
Aor51HI AGCGCT 1 cut(s) 1620
AoxI GGCC 5 cut(s) 568, 2702, 2774, 3134, 3626
ApaI GGGCCC 1 cut(s) 2706
ApeKI GCWGC 8 cut(s) 107, 1627, 1693, 3359, 3490, 3493, 3517, 3532
ApoI RAATTY 8 cut(s) 865, 1582, 1642, 1786, 1915, 2175, 2437, 3266
ArsI GACNNNNNNTTYG 2 cut(s) 3456, 3488
AseI ATTAAT 1 cut(s) 1752
AsiGI ACCGGT 1 cut(s) 3718
Asp700I GAANNNNTTC 2 cut(s) 842, 1586
AspS9I GGNCC 8 cut(s) 1304, 1492, 2702, 2703, 2774, 2876, 3032, 3333
AsuC2I CCSGG 8 cut(s) 712, 850, 1666, 1882, 2753, 3433, 3528, 3529
AsuII TTCGAA 1 cut(s) 3249
AvaI CYCGRG 3 cut(s) 2144, 3521, 3527
AvaII GGWCC 5 cut(s) 1304, 1492, 2876, 3032, 3333
AxyI CCTNAGG 1 cut(s) 3337
BaeGI GKGCMC 3 cut(s) 2102, 2706, 3123
BalI TGGCCA 1 cut(s) 3136
BanI GGYRCC 2 cut(s) 2387, 3144
BanII GRGCYC 6 cut(s) 543, 1206, 1686, 2217, 2706, 3528
BauI CACGAG 3 cut(s) 1222, 2727, 3053
BbsI GAAGAC 3 cut(s) 2001, 2921, 3471
Bbv12I GWGCWC 6 cut(s) 543, 1206, 1686, 2145, 2217, 2916
BbvCI CCTCAGC 2 cut(s) 1006, 3691
BbvI GCAGC 8 cut(s) 119, 1639, 1705, 3346, 3480, 3502, 3504, 3519
BccI CCATC 7 cut(s) 947, 2117, 2291, 2399, 2664, 2897, 2930
BcgI CGANNNNNNTGC 2 cut(s) 1422, 1456
BcnI CCSGG 8 cut(s) 712, 850, 1666, 1882, 2753, 3433, 3528, 3529
BcoDI GTCTC 3 cut(s) 560, 953, 2736
BfaI CTAG 1 cut(s) 3275
BfmI CTRYAG 2 cut(s) 1934, 3494
BfoI RGCGCY 3 cut(s) 1047, 1622, 2391
BfuAI ACCTGC 2 cut(s) 1768, 3020
BglI GCCNNNNNGGC 1 cut(s) 3142
BglII AGATCT 1 cut(s) 2362
BlpI GCTNAGC 5 cut(s) 614, 1233, 1697, 2381, 2858
Bme1390I CCNGG 8 cut(s) 712, 850, 1666, 1882, 2753, 3433, 3528, 3529
Bme18I GGWCC 5 cut(s) 1304, 1492, 2876, 3032, 3333
BmeT110I CYCGRG 3 cut(s) 2144, 3521, 3527
BmgBI CACGTC 3 cut(s) 1152, 3043, 3394
BmgT120I GGNCC 8 cut(s) 1304, 1492, 2702, 2703, 2774, 2876, 3032, 3333
BmrFI CCNGG 8 cut(s) 712, 850, 1666, 1882, 2753, 3433, 3528, 3529
BmrI ACTGGG 2 cut(s) 1455, 2482
BmsI GCATC 7 cut(s) 404, 463, 1083, 1680, 3004, 3113, 3229
BmuI ACTGGG 2 cut(s) 1455, 2482
BpiI GAAGAC 3 cut(s) 2001, 2921, 3471
BpmI CTGGAG 3 cut(s) 872, 1974, 2481
Bpu10I CCTNAGC 3 cut(s) 1006, 2737, 3691
Bpu1102I GCTNAGC 5 cut(s) 614, 1233, 1697, 2381, 2858
Bpu14I TTCGAA 1 cut(s) 3249
BpuEI CTTGAG 3 cut(s) 865, 935, 1950
BpuMI CCSGG 8 cut(s) 712, 850, 1666, 1882, 2753, 3433, 3528, 3529
BsaAI YACGTR 3 cut(s) 2808, 3124, 3346
BsaBI GATNNNNATC 2 cut(s) 1193, 2301
BsaHI GRCGYC 3 cut(s) 117, 2388, 2926
BsaI GGTCTC 1 cut(s) 953
BsaJI CCNNGG 7 cut(s) 562, 849, 1881, 2752, 3016, 3169, 3527
BsaWI WCCGGW 4 cut(s) 403, 805, 1410, 3718
Bsc4I CCNNNNNNNGG 6 cut(s) 387, 535, 711, 1417, 2376, 3045
Bse118I RCCGGY 6 cut(s) 724, 1387, 1891, 3279, 3442, 3718
Bse1I ACTGG 8 cut(s) 506, 1453, 1461, 1710, 1860, 2029, 2488, 2787
Bse21I CCTNAGG 1 cut(s) 3337
Bse3DI GCAATG 5 cut(s) 1227, 2330, 2520, 3150, 3162
Bse8I GATNNNNATC 2 cut(s) 1193, 2301
BseDI CCNNGG 7 cut(s) 562, 849, 1881, 2752, 3016, 3169, 3527
BseGI GGATG 3 cut(s) 478, 2302, 2391
BseJI GATNNNNATC 2 cut(s) 1193, 2301
BseLI CCNNNNNNNGG 6 cut(s) 387, 535, 711, 1417, 2376, 3045
BseMI GCAATG 5 cut(s) 1227, 2330, 2520, 3150, 3162
BseMII CTCAG 8 cut(s) 628, 1020, 1052, 1224, 1712, 2372, 2751, 3705
BseNI ACTGG 8 cut(s) 506, 1453, 1461, 1710, 1860, 2029, 2488, 2787
BsePI GCGCGC 1 cut(s) 456
BseRI GAGGAG 8 cut(s) 153, 181, 1433, 2263, 2270, 2402, 2858, 3041
BseSI GKGCMC 3 cut(s) 2102, 2706, 3123
BseX3I CGGCCG 2 cut(s) 568, 3626
BseXI GCAGC 8 cut(s) 119, 1639, 1705, 3346, 3480, 3502, 3504, 3519
BseYI CCCAGC 5 cut(s) 183, 246, 292, 468, 2479
BsgI GTGCAG 1 cut(s) 2577
Bsh1236I CGCG 3 cut(s) 458, 600, 3171
Bsh1285I CGRYCG 5 cut(s) 571, 1086, 1197, 3330, 3629
BshFI GGCC 5 cut(s) 570, 2704, 2776, 3136, 3628
BshNI GGYRCC 2 cut(s) 2387, 3144
BshTI ACCGGT 1 cut(s) 3718
BsiEI CGRYCG 5 cut(s) 571, 1086, 1197, 3330, 3629
BsiHKAI GWGCWC 6 cut(s) 543, 1206, 1686, 2145, 2217, 2916
BsiHKCI CYCGRG 3 cut(s) 2144, 3521, 3527
BslFI GGGAC 4 cut(s) 1130, 1946, 2402, 3334
BslI CCNNNNNNNGG 6 cut(s) 387, 535, 711, 1417, 2376, 3045
BsmAI GTCTC 3 cut(s) 560, 953, 2736
BsmBI CGTCTC 1 cut(s) 560
BsmFI GGGAC 4 cut(s) 1130, 1946, 2402, 3334
BsmI GAATGC 1 cut(s) 3179
BsnI GGCC 5 cut(s) 570, 2704, 2776, 3136, 3628
Bso31I GGTCTC 1 cut(s) 953
BsoBI CYCGRG 3 cut(s) 2144, 3521, 3527
Bsp119I TTCGAA 1 cut(s) 3249
Bsp120I GGGCCC 1 cut(s) 2702
Bsp1720I GCTNAGC 5 cut(s) 614, 1233, 1697, 2381, 2858
BspANI GGCC 5 cut(s) 570, 2704, 2776, 3136, 3628
BspCNI CTCAG 8 cut(s) 627, 1019, 1053, 1225, 1713, 2373, 2750, 3704
BspFNI CGCG 3 cut(s) 458, 600, 3171
BspHI TCATGA 1 cut(s) 1686
BspMAI CTGCAG 2 cut(s) 1938, 3498
BspMI ACCTGC 2 cut(s) 1768, 3020
BspPI GGATC 4 cut(s) 403, 2492, 3608, 3692
BspQI GCTCTTC 1 cut(s) 1763
BspT104I TTCGAA 1 cut(s) 3249
BspT107I GGYRCC 2 cut(s) 2387, 3144
BspTNI GGTCTC 1 cut(s) 953
BsrBI CCGCTC 2 cut(s) 383, 2385
BsrDI GCAATG 5 cut(s) 1227, 2330, 2520, 3150, 3162
BsrFI RCCGGY 6 cut(s) 724, 1387, 1891, 3279, 3442, 3718
BsrI ACTGG 8 cut(s) 506, 1453, 1461, 1710, 1860, 2029, 2488, 2787
BssAI RCCGGY 6 cut(s) 724, 1387, 1891, 3279, 3442, 3718
BssECI CCNNGG 7 cut(s) 562, 849, 1881, 2752, 3016, 3169, 3527
BssHII GCGCGC 1 cut(s) 456
BssNAI GTATAC 1 cut(s) 243
BssNI GRCGYC 3 cut(s) 117, 2388, 2926
BssSI CACGAG 3 cut(s) 1222, 2727, 3053
Bst1107I GTATAC 1 cut(s) 243
Bst2BI CACGAG 3 cut(s) 1222, 2727, 3053
Bst4CI ACNGT 4 cut(s) 58, 1308, 2356, 3010
Bst6I CTCTTC 4 cut(s) 335, 1763, 3096, 3561
BstACI GRCGYC 3 cut(s) 117, 2388, 2926
BstBAI YACGTR 3 cut(s) 2808, 3124, 3346
BstBI TTCGAA 1 cut(s) 3249
BstDSI CCRYGG 1 cut(s) 3169
BstENI CCTNNNNNAGG 1 cut(s) 2374
BstF5I GGATG 3 cut(s) 478, 2302, 2391
BstFNI CGCG 3 cut(s) 458, 600, 3171
BstH2I RGCGCY 3 cut(s) 1047, 1622, 2391
BstMAI GTCTC 3 cut(s) 560, 953, 2736
BstMCI CGRYCG 5 cut(s) 571, 1086, 1197, 3330, 3629
BstNSI RCATGY 3 cut(s) 1372, 2008, 3070
BstSCI CCNGG 8 cut(s) 710, 848, 1664, 1880, 2751, 3431, 3526, 3527
BstSFI CTRYAG 2 cut(s) 1934, 3494
BstSLI GKGCMC 3 cut(s) 2102, 2706, 3123
BstUI CGCG 3 cut(s) 458, 600, 3171
BstV1I GCAGC 8 cut(s) 119, 1639, 1705, 3346, 3480, 3502, 3504, 3519
BstV2I GAAGAC 3 cut(s) 2001, 2921, 3471
BstX2I RGATCY 2 cut(s) 408, 2362
BstXI CCANNNNNNTGG 1 cut(s) 992
BstYI RGATCY 2 cut(s) 408, 2362
BstZ17I GTATAC 1 cut(s) 243
BstZI CGGCCG 2 cut(s) 568, 3626
Bsu36I CCTNAGG 1 cut(s) 3337
BsuRI GGCC 5 cut(s) 570, 2704, 2776, 3136, 3628
BtgI CCRYGG 1 cut(s) 3169
BtgZI GCGATG 2 cut(s) 1106, 2683
BtrI CACGTC 3 cut(s) 1152, 3043, 3394
BtsCI GGATG 3 cut(s) 478, 2302, 2391
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 5 cut(s) 63, 196, 1468, 1853, 2361
BveI ACCTGC 2 cut(s) 1768, 3020
CaiI CAGNNNCTG 1 cut(s) 1142
CciI TCATGA 1 cut(s) 1686
Cfr10I RCCGGY 6 cut(s) 724, 1387, 1891, 3279, 3442, 3718
Cfr13I GGNCC 8 cut(s) 1304, 1492, 2702, 2703, 2774, 2876, 3032, 3333
Cfr42I CCGCGG 1 cut(s) 3172
Cfr9I CCCGGG 1 cut(s) 3527
CseI GACGC 3 cut(s) 89, 106, 2915
Csp6I GTAC 4 cut(s) 1034, 3058, 3125, 3203
CspAI ACCGGT 1 cut(s) 3718
CviAII CATG 7 cut(s) 304, 1369, 1687, 2005, 3067, 3193, 3652
CviQI GTAC 4 cut(s) 1034, 3058, 3125, 3203
DinI GGCGCC 1 cut(s) 2389
EaeI YGGCCR 3 cut(s) 568, 3134, 3626
EagI CGGCCG 2 cut(s) 568, 3626
Eam1104I CTCTTC 4 cut(s) 335, 1763, 3096, 3561
EarI CTCTTC 4 cut(s) 335, 1763, 3096, 3561
EciI GGCGGA 4 cut(s) 533, 862, 1406, 2689
Ecl136II GAGCTC 4 cut(s) 541, 1204, 1684, 2215
EclXI CGGCCG 2 cut(s) 568, 3626
Eco24I GRGCYC 6 cut(s) 543, 1206, 1686, 2217, 2706, 3528
Eco31I GGTCTC 1 cut(s) 953
Eco32I GATATC 2 cut(s) 1246, 2188
Eco47I GGWCC 5 cut(s) 1304, 1492, 2876, 3032, 3333
Eco47III AGCGCT 1 cut(s) 1620
Eco52I CGGCCG 2 cut(s) 568, 3626
Eco53kI GAGCTC 4 cut(s) 541, 1204, 1684, 2215
Eco57I CTGAAG 3 cut(s) 1088, 2994, 3375
Eco81I CCTNAGG 1 cut(s) 3337
Eco88I CYCGRG 3 cut(s) 2144, 3521, 3527
EcoICRI GAGCTC 4 cut(s) 541, 1204, 1684, 2215
EcoNI CCTNNNNNAGG 1 cut(s) 2374
EcoO109I RGGNCCY 5 cut(s) 1492, 2702, 2703, 3032, 3333
EcoRI GAATTC 1 cut(s) 3266
EcoRV GATATC 2 cut(s) 1246, 2188
EcoT38I GRGCYC 6 cut(s) 543, 1206, 1686, 2217, 2706, 3528
EgeI GGCGCC 1 cut(s) 2389
EheI GGCGCC 1 cut(s) 2389
Esp3I CGTCTC 1 cut(s) 560
FaeI CATG 7 cut(s) 307, 1372, 1690, 2008, 3070, 3196, 3655
FalI AAGNNNNNCTT 2 cut(s) 3579, 3611
FaqI GGGAC 4 cut(s) 1130, 1946, 2402, 3334
FatI CATG 7 cut(s) 303, 1368, 1686, 2004, 3066, 3192, 3651
FauI CCCGC 5 cut(s) 388, 456, 729, 1793, 3176
FauNDI CATATG 1 cut(s) 112
FblI GTMKAC 2 cut(s) 242, 3317
FokI GGATG 3 cut(s) 485, 2309, 2378
FriOI GRGCYC 6 cut(s) 543, 1206, 1686, 2217, 2706, 3528
FspBI CTAG 1 cut(s) 3275
GsaI CCCAGC 5 cut(s) 187, 250, 296, 472, 2483
GsuI CTGGAG 3 cut(s) 872, 1974, 2481
HaeII RGCGCY 3 cut(s) 1047, 1622, 2391
HaeIII GGCC 5 cut(s) 570, 2704, 2776, 3136, 3628
HgaI GACGC 3 cut(s) 89, 106, 2915
Hin1I GRCGYC 3 cut(s) 117, 2388, 2926
Hin1II CATG 7 cut(s) 307, 1372, 1690, 2008, 3070, 3196, 3655
HincII GTYRAC 4 cut(s) 898, 1323, 1382, 2238
HindII GTYRAC 4 cut(s) 898, 1323, 1382, 2238
HindIII AAGCTT 5 cut(s) 1100, 1844, 1874, 2681, 3306
HpyCH4III ACNGT 4 cut(s) 58, 1308, 2356, 3010
Hsp92I GRCGYC 3 cut(s) 117, 2388, 2926
Hsp92II CATG 7 cut(s) 307, 1372, 1690, 2008, 3070, 3196, 3655
KasI GGCGCC 1 cut(s) 2387
KroI GCCGGC 1 cut(s) 724
KroNI GCCGGC 1 cut(s) 726
KspI CCGCGG 1 cut(s) 3172
LguI GCTCTTC 1 cut(s) 1763
Lsp1109I GCAGC 8 cut(s) 119, 1639, 1705, 3346, 3480, 3502, 3504, 3519
LweI GCATC 7 cut(s) 404, 463, 1083, 1680, 3004, 3113, 3229
MaeI CTAG 1 cut(s) 3275
MaeIII GTNAC 8 cut(s) 518, 675, 1119, 1330, 1485, 1724, 2225, 2695
MbiI CCGCTC 2 cut(s) 383, 2385
MfeI CAATTG 1 cut(s) 1795
MflI RGATCY 2 cut(s) 408, 2362
MlsI TGGCCA 1 cut(s) 3136
MluNI TGGCCA 1 cut(s) 3136
Mly113I GGCGCC 1 cut(s) 2388
MlyI GAGTC 3 cut(s) 872, 971, 1175
MmeI TCCRAC 9 cut(s) 213, 1041, 1100, 1395, 1738, 2274, 2289, 3399, 3537
Mox20I TGGCCA 1 cut(s) 3136
MreI CGCCGGCG 1 cut(s) 724
MroNI GCCGGC 1 cut(s) 724
MroXI GAANNNNTTC 2 cut(s) 842, 1586
MscI TGGCCA 1 cut(s) 3136
MseI TTAA 8 cut(s) 222, 374, 419, 429, 1283, 1752, 3197, 3428
MslI CAYNNNNRTG 1 cut(s) 806
Msp20I TGGCCA 1 cut(s) 3136
MspA1I CMGCKG 6 cut(s) 988, 2479, 3171, 3493, 3631, 3695
MspR9I CCNGG 8 cut(s) 712, 850, 1666, 1882, 2753, 3433, 3528, 3529
MunI CAATTG 1 cut(s) 1795
Mva1269I GAATGC 1 cut(s) 3179
MvnI CGCG 3 cut(s) 458, 600, 3171
NaeI GCCGGC 1 cut(s) 726
NarI GGCGCC 1 cut(s) 2388
NciI CCSGG 8 cut(s) 712, 850, 1666, 1882, 2753, 3433, 3528, 3529
NdeI CATATG 1 cut(s) 112
NgoMIV GCCGGC 1 cut(s) 724
NlaIII CATG 7 cut(s) 307, 1372, 1690, 2008, 3070, 3196, 3655
NmeAIII GCCGAG 3 cut(s) 543, 2961, 3138
NmuCI GTSAC 4 cut(s) 518, 1119, 1330, 2225
NspI RCATGY 3 cut(s) 1372, 2008, 3070
NspV TTCGAA 1 cut(s) 3249
PaeR7I CTCGAG 2 cut(s) 2144, 3521
PagI TCATGA 1 cut(s) 1686
PauI GCGCGC 1 cut(s) 456
PciI ACATGT 2 cut(s) 1368, 2004
PciSI GCTCTTC 1 cut(s) 1763
PcsI WCGNNNNNNNCGW 5 cut(s) 1088, 1190, 3207, 3234, 3374
PctI GAATGC 1 cut(s) 3179
PdiI GCCGGC 1 cut(s) 726
PdmI GAANNNNTTC 2 cut(s) 842, 1586
PflFI GACNNNGTC 1 cut(s) 3470
PfoI TCCNGGA 1 cut(s) 710
PinAI ACCGGT 1 cut(s) 3718
Ple19I CGATCG 3 cut(s) 1086, 1197, 3330
PleI GAGTC 3 cut(s) 872, 971, 1175
PluTI GGCGCC 1 cut(s) 2391
PpsI GAGTC 3 cut(s) 872, 971, 1175
Ppu21I YACGTR 3 cut(s) 2808, 3124, 3346
PpuMI RGGWCCY 3 cut(s) 1492, 3032, 3333
PscI ACATGT 2 cut(s) 1368, 2004
PshBI ATTAAT 1 cut(s) 1752
Psp124BI GAGCTC 4 cut(s) 543, 1206, 1686, 2217
Psp1406I AACGTT 1 cut(s) 3228
Psp5II RGGWCCY 3 cut(s) 1492, 3032, 3333
PspFI CCCAGC 5 cut(s) 183, 246, 292, 468, 2479
PspOMI GGGCCC 1 cut(s) 2702
PspPI GGNCC 8 cut(s) 1304, 1492, 2702, 2703, 2774, 2876, 3032, 3333
PspPPI RGGWCCY 3 cut(s) 1492, 3032, 3333
PspXI VCTCGAGB 2 cut(s) 2144, 3521
PsrI GAACNNNNNNTAC 2 cut(s) 1257, 1289
PstI CTGCAG 2 cut(s) 1938, 3498
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 2 cut(s) 408, 2362
PsyI GACNNNGTC 1 cut(s) 3470
PteI GCGCGC 1 cut(s) 456
PvuI CGATCG 3 cut(s) 1086, 1197, 3330
PvuII CAGCTG 3 cut(s) 988, 3493, 3695
RsaI GTAC 4 cut(s) 1035, 3059, 3126, 3204
RsaNI GTAC 4 cut(s) 1034, 3058, 3125, 3203
RseI CAYNNNNRTG 1 cut(s) 806
SacI GAGCTC 4 cut(s) 543, 1206, 1686, 2217
SacII CCGCGG 1 cut(s) 3172
SapI GCTCTTC 1 cut(s) 1763
SaqAI TTAA 8 cut(s) 222, 374, 419, 429, 1283, 1752, 3197, 3428
Sau96I GGNCC 8 cut(s) 1304, 1492, 2702, 2703, 2774, 2876, 3032, 3333
SchI GAGTC 3 cut(s) 872, 971, 1175
ScrFI CCNGG 8 cut(s) 712, 850, 1666, 1882, 2753, 3433, 3528, 3529
SfaNI GCATC 7 cut(s) 404, 463, 1083, 1680, 3004, 3113, 3229
SfcI CTRYAG 2 cut(s) 1934, 3494
SfoI GGCGCC 1 cut(s) 2389
Sfr274I CTCGAG 2 cut(s) 2144, 3521
Sfr303I CCGCGG 1 cut(s) 3172
SfuI TTCGAA 1 cut(s) 3249
SgrAI CRCCGGYG 1 cut(s) 724
SgrBI CCGCGG 1 cut(s) 3172
SinI GGWCC 5 cut(s) 1304, 1492, 2876, 3032, 3333
SlaI CTCGAG 2 cut(s) 2144, 3521
SmaI CCCGGG 1 cut(s) 3529
SmiMI CAYNNNNRTG 1 cut(s) 806
SmlI CTYRAG 5 cut(s) 880, 950, 1965, 2144, 3521
SmoI CTYRAG 5 cut(s) 880, 950, 1965, 2144, 3521
SrfI GCCCGGGC 1 cut(s) 3529
SspDI GGCGCC 1 cut(s) 2387
SspMI CTAG 1 cut(s) 3275
SstI GAGCTC 4 cut(s) 543, 1206, 1686, 2217
StyD4I CCNGG 8 cut(s) 710, 848, 1664, 1880, 2751, 3431, 3526, 3527
TaaI ACNGT 4 cut(s) 58, 1308, 2356, 3010
TauI GCSGC 6 cut(s) 368, 570, 2388, 2479, 3145, 3631
Tru1I TTAA 8 cut(s) 222, 374, 419, 429, 1283, 1752, 3197, 3428
Tru9I TTAA 8 cut(s) 222, 374, 419, 429, 1283, 1752, 3197, 3428
TscAI CASTG 5 cut(s) 63, 203, 1468, 1860, 2361
TseFI GTSAC 4 cut(s) 518, 1119, 1330, 2225
TseI GCWGC 8 cut(s) 107, 1627, 1693, 3359, 3490, 3493, 3517, 3532
Tsp45I GTSAC 4 cut(s) 518, 1119, 1330, 2225
TspDTI ATGAA 7 cut(s) 322, 822, 1856, 2531, 2964, 3300, 3640
TspGWI ACGGA 4 cut(s) 136, 1138, 2061, 3675
TspMI CCCGGG 1 cut(s) 3527
TspRI CASTG 5 cut(s) 63, 203, 1468, 1860, 2361
Tth111I GACNNNGTC 1 cut(s) 3470
VpaK11BI GGWCC 5 cut(s) 1304, 1492, 2876, 3032, 3333
VspI ATTAAT 1 cut(s) 1752
XagI CCTNNNNNAGG 1 cut(s) 2374
XapI RAATTY 8 cut(s) 865, 1582, 1642, 1786, 1915, 2175, 2437, 3266
XceI RCATGY 3 cut(s) 1372, 2008, 3070
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XhoI CTCGAG 2 cut(s) 2144, 3521
XmaI CCCGGG 1 cut(s) 3527
XmiI GTMKAC 2 cut(s) 242, 3317
XmnI GAANNNNTTC 2 cut(s) 842, 1586
XspI CTAG 1 cut(s) 3275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.