RchiOBHm_Chr6g0302191

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011850
Physical Location & Seq
Forward (+)
62334387 .. 62338156
3770 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27145

Sequence Viewer

Length: 3333 bp
ATGGCAACGGCTAGTTTCTTCTTTCTGATCATCCCAATGCTGTACTTCACCGCCGCATTGACCGCCGGTCAACTATTACTCTCCGAGATCCAAGCCTTAACCACCTTCAAGCTCAACCTCCACGACCCGCTCGGCGCCCTCAACGGCTGGGACTCCTCCACGCCATCCGCCCCATGCGACTGGCGCGGCGTCGTTTGCTTCAACAACCGAGTCCGGGAACTCAGCCTGCCTCGCCTCCACCTCGCGGGTCGACTCGGCGACCAACTCGCCAGCCTCCGTGACCTCCGCAAGCTCAACCTCCACACCAACAACTTCAACGGCTCCATTCCTCCCTCCCTCTCCCAATGCGCCTTGCTACGCACCGTTTACTTGCATGGCAACTCACTCTCCGGCGACCTCCCGCCGGCGCTTCTCAACCTCACCAACCTCCAAATCCTCAACCTCGCTCACAACTCCCTCTCCGGCAGAATCTCCGGCGACATTCCGGCCAGCCTCCGATACCTTGATCTTTCCTCCAATGCATTCACCGGGGACATTCCGGTGAACTTCTCCGCCAACTCGCAGCTCCAGCTCGTCAATTTTTCCTTCAATCAGTTCACCGGCGGCGTTCCGCCGACAATCGGACGGCTCCAGATGCTTGAGTATCTCTGGCTGGACTCGAACCAGCTGACGGGCACGCTTCCTTCGGCTATTTCCAACTGCTCCTCCCTCATACATTTCAGCGCCGACGACAACGCGCTCCGAGGACTAATTCCGGCGACTATCGGCGCCATTCCGAAGCTTCAGGTGGTTTCTTTAGCTCGGAACAGCTTATCAGGCTCAGTCCCGATAGCTTTATTGTGCAATGGGAATGTTGACTCTTCTTCACTGAGGATAGTAGATCTAGGGTTCAATGCGCTCACGGGAATTGTGCAGCCGCAAAATGGAAGGTGTTCGAGTATTCTGGAGGGTTTTCGATGTCGCTGGAAACTCGTTTTCGGGGGATTGCCGACCGAAATCGGCAATCTTTTCCGGTTGGAAGAACTCCGGGTGGGTAATAATTCCCTGAGCGGTGAAATTCCTACTGAGATTGCAAAATGCAGTTTGTTACAGGCTCTTGATCTTGAAGGGAACCGCTTTGAGGGCCAGTTTCCGTTGTTTCTGGGAGGAATGAGGAGCTTAAAGGTGTTGTCCTTGGGTGGGAATTTAATCTCGGGTACCATCCCGTCGAGTTTGGGAGCACTGTCGGGGCTAGAATCCTTGAACCTGAGCGGTAATAATCTGACAGGGGAAGTTCCAGTTGAGCTCATGCAGCTAAGCAACTTGACTAGCCTGAACCTCAGTAATAACAAGTTTTCCGGTGAGGTTCTTGGGAATGTAGGAGCATTGAGGAGCTTGCAGGGTTTGAATTTGAGCAATTGTGGGCTTTCAGGTAGCGTCCCTGCGAGTGTTGGAAGTTTGATGAGGCTCGCTACACTGGATTTGAGTAAACAAAAGCTCTCTGGTGAGTTGCCTACTGAGATTTTTGGGCTGCCAAATTTACAAGTGGTTGCTTTGCAAGAGAATCAGTTGTCTGGGGCTGTCCCTCAAGGTTTTAGTAGCTTGACTGGTTTAGTGTACCTAAATCTCTCCTCCAATGCCTTTACTGGTGTTATTCCAGCCACGTATGGGTATCTACGGTCACTAACTGTTCTATCTTTGTCAAAGAATCGCATTTCCGGTGTGATCCCACCAGAGCTTGGAGATTGTTTGAACCTTCAAGTACTTGAGCTTAGTTCTAATCAATTGGGGGACAAAATTCCAGTTGATATCTCTCGGCTATCTAGTTTGGAAGAGCTGGATTTGGGTCATAACAAATTGACAGGTGAAATACCAGAGGAAATTTCCAAATGCTCTGCTCTGACTTCATTATTGTTAGAGGAAAATCAACTTTCAGGGCCAATACCAAACTCCTTGCCTTTGTTAGTGAACCTTGTGGTGTTAGATCTTTCCTCAAACCAGTTGATTGGAGCAATCCCGGCAAATATTTCACTCATTCCTCACTTGAAGTCCTTGAATTTGTCTAACAACAACCTATCTGGTAAGATTCCAGAGCTACTGGGTTCTCAATTCAATGGGTCTGTCTTTGCAATGAACCCGAAATTATGTGGGAAACCATTGGACAGAGAATGTGGAGATGCGAAAAGGAGAAGAAGAAACAGGTTGCTTTTATTAATTGGGGTGGCTGCTGGTGGAGCTTTGCTTCTGTTAATGTGTTGTTGTGGCTATGTCTACAGCCTCTTCCGCTGGCGTAAGAAGCTTAGAGAAATGGTAACTGGGGAGAAGAAGGGTCCCAGTCCAGGCCGTGCAAGCTCAGGCGGAGAGAGATCTGGTCGTGGAAGTGGAGACAATGGAGGGCCAAAGCTTGTTATGTTCAATACTAAGATTACATATGCAGAAGCATTAGAAGCAACAAGACAATTTGATGAGGAAAATGTGTTAAGCAGGGGAAAGTACGGACTGGTATTCAAGGCTTCATTCCCAGATGGAATGGTGCTCTCAATTCGACGCCTTCCTGCTTCTTCTCTTGGTGTGCTCGATGAAGAGACGTTCCACAAAGAAGCCGAAGCTCTTGGCAAGGTGAAGCATCGGAATTTAACAGTTCTGCGTGGCTACTATGCCGGTCCTGATGTGAGGCTACTTGTATACGACTACATGCCTAATGGAAACTTAGCCACTCTGCTCCAAGAGGCTTCTCACCAAGATGGTCATGTACTAAATTGGCCAATGCGGCATCTCATTGCACTTGGCATTGCTAGAGGCATAGCTTTCCTGCACTCAGTCTCAATGGTTCATGGTGATGTGAAGCCGCAAAATGTTCTCTTCGACGCTGATTTTGAAGCCCACTTATCGGAATTTGGATTAGACCGGTTAACCATAGTCTCACCAAAAGCCTCCTCATCATCATCTGCCGCAATTGGTACATTAGGCTATGTGTCACCCGAAGCAGCACTAACCGGGCAGGCAACAAAAGAAGCCGATGTGTATAGTTTCGGAATTGTCCTGTTGGAGATCCTAACCGGAAGGAAGCCAGTGATGTTTACTGAAGAGGAAGACATAGTGAAATGGGTGAAGAAGCAACTACAAAGAGGTCAAATCACAGAGCTGCTAGAGCCTGGACTACTTGAGCTAGACCCGGAGTCATCAGAATCAGAAGAGTTCTTATTGGGAGTAAAAGTTGGACTACTCTGCACCGCACCTGATCCACTTGATAGACCCCTCATGGCTGACATTGTGTTCATGCTTGAAGGTTGCCGCGTCGGACCGGACATACCATCGTCGGCTGATCCAACTTCACTACCTTCACCGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1110

Amino Acids

119.04

Weight (kDa)

5.98

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 66 6.5e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 332 - 442 7.2e-09 Leucine-rich repeat region
LRR_8 PF13855 382 - 421 4.3e-06 Leucine rich repeat
LRR_14 PF23598 457 - 587 5.5e-11 Leucine-rich repeat region
LRR_8 PF13855 482 - 540 5.6e-08 Leucine rich repeat
LRR_8 PF13855 535 - 589 8.8e-07 Leucine rich repeat
LRR_4 PF12799 554 - 591 9e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 598 - 637 7.4e-06 Leucine rich repeat
Pkinase PF00069 816 - 1036 4.1e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 818 - 1035 5.1e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75640
fragaria_vesca FvH4_2g39080
malus_domestica MD08G1017600.v1.1 MD08G1017800.v1.1 MD15G1016500.v1.1
prunus_persica Prupe.1G370300_v2.0.a1
pyrus_communis pycom08g01540 pycom15g01450 pycom15g01460
rosa_chinensis RchiOBHm_Chr6g0302191
rosa_laevigata RLG00000011188
rosa_multiflora Rmu_co8058424.1_g000001
rosa_roxburghii Rroxscaffold_7G00166010
rosa_rugosa Rorug06G0313000
rosa_samantha Rh6AG425500 Rh6BG477000 Rh6CG438300 Rh6DG425300
rosa_wichuraiana Rw6G036850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1196
AccB1I GGYRCC 3 cut(s) 134, 767, 1196
AccB7I CCANNNNNTGG 1 cut(s) 1718
AccBSI CCGCTC 3 cut(s) 130, 1050, 1251
AccI GTMKAC 3 cut(s) 250, 2250, 2664
AccII CGCG 4 cut(s) 186, 245, 737, 3276
AclWI GGATC 5 cut(s) 82, 1699, 3025, 3215, 3299
AcoI YGGCCR 2 cut(s) 486, 2741
AcsI RAATTY 9 cut(s) 1056, 1183, 1387, 1516, 1776, 1860, 2035, 2611, 2873
AcuI CTGAAG 2 cut(s) 767, 3084
AcyI GRCGYC 4 cut(s) 135, 189, 768, 2527
AfaI GTAC 7 cut(s) 44, 1198, 1598, 1743, 2474, 2733, 2941
AgeI ACCGGT 1 cut(s) 2886
AhdI GACNNNNNGTC 2 cut(s) 66, 3157
AjnI CCWGG 2 cut(s) 2317, 3133
Alw21I GWGCWC 4 cut(s) 1222, 1287, 2517, 2556
Alw26I GTCTC 4 cut(s) 2358, 2558, 2806, 2905
AlwI GGATC 5 cut(s) 82, 1699, 3025, 3215, 3299
Ama87I CYCGRG 1 cut(s) 1192
AoxI GGCC 7 cut(s) 486, 1123, 1916, 2320, 2375, 2741, 3327
ApeKI GCWGC 7 cut(s) 562, 913, 1291, 1510, 2204, 2966, 3124
ApoI RAATTY 9 cut(s) 1056, 1183, 1387, 1516, 1776, 1860, 2035, 2611, 2873
ArsI GACNNNNNNTTYG 2 cut(s) 2837, 2869
AseI ATTAAT 1 cut(s) 2192
AsiGI ACCGGT 1 cut(s) 2886
Asp700I GAANNNNTTC 1 cut(s) 931
Asp718I GGTACC 1 cut(s) 1196
AspLEI GCGC 8 cut(s) 137, 186, 350, 409, 725, 739, 770, 898
AspS9I GGNCC 6 cut(s) 1123, 1916, 2309, 2375, 2642, 3281
AsuC2I CCSGG 6 cut(s) 215, 529, 1028, 1997, 2977, 3155
AvaI CYCGRG 1 cut(s) 1192
AvaII GGWCC 3 cut(s) 2309, 2642, 3281
BaeGI GKGCMC 1 cut(s) 677
BalI TGGCCA 1 cut(s) 2743
BanI GGYRCC 3 cut(s) 134, 767, 1196
BanII GRGCYC 1 cut(s) 1287
BbsI GAAGAC 1 cut(s) 3078
Bbv12I GWGCWC 4 cut(s) 1222, 1287, 2517, 2556
BbvI GCAGC 7 cut(s) 574, 925, 1303, 1497, 2191, 2978, 3111
BccI CCATC 5 cut(s) 172, 1208, 2498, 2717, 3301
BceAI ACGGC 5 cut(s) 24, 160, 334, 641, 2307
BciT130I CCWGG 2 cut(s) 2319, 3135
BclI TGATCA 1 cut(s) 27
BcnI CCSGG 6 cut(s) 215, 529, 1028, 1997, 2977, 3155
BcoDI GTCTC 4 cut(s) 2358, 2558, 2806, 2905
BfaI CTAG 8 cut(s) 12, 884, 1232, 1308, 1803, 2775, 3128, 3149
BfmI CTRYAG 1 cut(s) 2251
BfoI RGCGCY 4 cut(s) 138, 410, 726, 771
BglI GCCNNNNNGGC 1 cut(s) 2749
BglII AGATCT 3 cut(s) 880, 1963, 2345
BlpI GCTNAGC 1 cut(s) 1295
BmcAI AGTACT 1 cut(s) 1743
Bme1390I CCNGG 8 cut(s) 215, 529, 1028, 1997, 2319, 2977, 3135, 3155
Bme18I GGWCC 3 cut(s) 2309, 2642, 3281
BmeRI GACNNNNNGTC 2 cut(s) 66, 3157
BmeT110I CYCGRG 1 cut(s) 1192
BmgT120I GGNCC 6 cut(s) 1123, 1916, 2309, 2375, 2642, 3281
BmiI GGNNCC 8 cut(s) 136, 322, 629, 769, 1112, 1198, 2310, 2311
BmrFI CCNGG 8 cut(s) 215, 529, 1028, 1997, 2319, 2977, 3135, 3155
BmrI ACTGGG 3 cut(s) 2087, 2304, 2307
BmsI GCATC 4 cut(s) 624, 2146, 2614, 2761
BmuI ACTGGG 3 cut(s) 2087, 2304, 2307
BpiI GAAGAC 1 cut(s) 3078
BpmI CTGGAG 3 cut(s) 551, 614, 965
Bpu10I CCTNAGC 3 cut(s) 1046, 1247, 2332
Bpu1102I GCTNAGC 1 cut(s) 1295
BpuEI CTTGAG 4 cut(s) 659, 1551, 1766, 3164
BpuMI CCSGG 6 cut(s) 215, 529, 1028, 1997, 2977, 3155
BsaAI YACGTR 1 cut(s) 1644
BsaHI GRCGYC 4 cut(s) 135, 189, 768, 2527
BsaJI CCNNGG 3 cut(s) 528, 742, 1173
BsaWI WCCGGW 7 cut(s) 538, 1011, 1337, 1697, 2886, 3038, 3283
BsaXI ACNNNNNCTCC 8 cut(s) 371, 401, 443, 473, 689, 719, 3180, 3210
Bse118I RCCGGY 6 cut(s) 65, 403, 599, 2639, 2886, 3325
Bse3DI GCAATG 4 cut(s) 850, 2115, 2757, 2769
BseBI CCWGG 2 cut(s) 2319, 3135
BseDI CCNNGG 3 cut(s) 528, 742, 1173
BseGI GGATG 3 cut(s) 30, 164, 1200
BseMI GCAATG 4 cut(s) 850, 2115, 2757, 2769
BseRI GAGGAG 6 cut(s) 145, 694, 1168, 1384, 1600, 2905
BseSI GKGCMC 1 cut(s) 677
BseXI GCAGC 7 cut(s) 574, 925, 1303, 1497, 2191, 2978, 3111
BseYI CCCAGC 1 cut(s) 147
BsgI GTGCAG 3 cut(s) 932, 2777, 3193
Bsh1236I CGCG 4 cut(s) 186, 245, 737, 3276
Bsh1285I CGRYCG 1 cut(s) 993
BshFI GGCC 7 cut(s) 488, 1125, 1918, 2322, 2377, 2743, 3329
BshNI GGYRCC 3 cut(s) 134, 767, 1196
BshTI ACCGGT 1 cut(s) 2886
BsiEI CGRYCG 1 cut(s) 993
BsiHKAI GWGCWC 4 cut(s) 1222, 1287, 2517, 2556
BsiHKCI CYCGRG 1 cut(s) 1192
BslFI GGGAC 7 cut(s) 164, 545, 809, 1403, 1547, 1784, 2295
BsmAI GTCTC 4 cut(s) 2358, 2558, 2806, 2905
BsmBI CGTCTC 1 cut(s) 2558
BsmFI GGGAC 7 cut(s) 164, 545, 809, 1403, 1547, 1784, 2295
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 7 cut(s) 488, 1125, 1918, 2322, 2377, 2743, 3329
BsoBI CYCGRG 1 cut(s) 1192
Bsp1286I GDGCHC 5 cut(s) 677, 1222, 1287, 2517, 2556
Bsp1720I GCTNAGC 1 cut(s) 1295
BspANI GGCC 7 cut(s) 488, 1125, 1918, 2322, 2377, 2743, 3329
BspFNI CGCG 4 cut(s) 186, 245, 737, 3276
BspLI GGNNCC 8 cut(s) 136, 322, 629, 769, 1112, 1198, 2310, 2311
BspPI GGATC 5 cut(s) 82, 1699, 3025, 3215, 3299
BspQI GCTCTTC 1 cut(s) 1806
BspT107I GGYRCC 3 cut(s) 134, 767, 1196
BsrBI CCGCTC 3 cut(s) 130, 1050, 1251
BsrDI GCAATG 4 cut(s) 850, 2115, 2757, 2769
BsrFI RCCGGY 6 cut(s) 65, 403, 599, 2639, 2886, 3325
BssAI RCCGGY 6 cut(s) 65, 403, 599, 2639, 2886, 3325
BssECI CCNNGG 3 cut(s) 528, 742, 1173
BssNAI GTATAC 1 cut(s) 2665
BssNI GRCGYC 4 cut(s) 135, 189, 768, 2527
BssT1I CCWWGG 1 cut(s) 1173
Bst1107I GTATAC 1 cut(s) 2665
Bst2UI CCWGG 2 cut(s) 2319, 3135
Bst4CI ACNGT 5 cut(s) 364, 1224, 1659, 1669, 2620
Bst6I CTCTTC 7 cut(s) 865, 1806, 2264, 2556, 2846, 3060, 3168
BstACI GRCGYC 4 cut(s) 135, 189, 768, 2527
BstBAI YACGTR 1 cut(s) 1644
BstF5I GGATG 3 cut(s) 30, 164, 1200
BstFNI CGCG 4 cut(s) 186, 245, 737, 3276
BstH2I RGCGCY 4 cut(s) 138, 410, 726, 771
BstHHI GCGC 8 cut(s) 137, 186, 350, 409, 725, 739, 770, 898
BstMAI GTCTC 4 cut(s) 2358, 2558, 2806, 2905
BstMCI CGRYCG 1 cut(s) 993
BstNI CCWGG 2 cut(s) 2319, 3135
BstNSI RCATGY 1 cut(s) 2677
BstSCI CCNGG 8 cut(s) 213, 527, 1026, 1995, 2317, 2975, 3133, 3153
BstSFI CTRYAG 1 cut(s) 2251
BstSLI GKGCMC 1 cut(s) 677
BstUI CGCG 4 cut(s) 186, 245, 737, 3276
BstV1I GCAGC 7 cut(s) 574, 925, 1303, 1497, 2191, 2978, 3111
BstV2I GAAGAC 1 cut(s) 3078
BstX2I RGATCY 5 cut(s) 87, 880, 1963, 2345, 3030
BstXI CCANNNNNNTGG 2 cut(s) 180, 1985
BstYI RGATCY 5 cut(s) 87, 880, 1963, 2345, 3030
BstZ17I GTATAC 1 cut(s) 2665
BsuRI GGCC 7 cut(s) 488, 1125, 1918, 2322, 2377, 2743, 3329
BtsCI GGATG 3 cut(s) 30, 164, 1200
BtsIMutI CAGTG 4 cut(s) 866, 1220, 1454, 3057
CfoI GCGC 8 cut(s) 137, 186, 350, 409, 725, 739, 770, 898
Cfr10I RCCGGY 6 cut(s) 65, 403, 599, 2639, 2886, 3325
Cfr13I GGNCC 6 cut(s) 1123, 1916, 2309, 2375, 2642, 3281
CpoI CGGWCCG 1 cut(s) 3281
CseI GACGC 5 cut(s) 178, 1405, 2535, 2855, 3265
Csp6I GTAC 7 cut(s) 43, 1197, 1597, 1742, 2473, 2732, 2940
CspAI ACCGGT 1 cut(s) 2886
CspI CGGWCCG 1 cut(s) 3281
CviAII CATG 8 cut(s) 174, 374, 1288, 2674, 2729, 2813, 3241, 3259
CviQI GTAC 7 cut(s) 43, 1197, 1597, 1742, 2473, 2732, 2940
DinI GGCGCC 2 cut(s) 136, 769
DriI GACNNNNNGTC 2 cut(s) 66, 3157
EaeI YGGCCR 2 cut(s) 486, 2741
Eam1104I CTCTTC 7 cut(s) 865, 1806, 2264, 2556, 2846, 3060, 3168
Eam1105I GACNNNNNGTC 2 cut(s) 66, 3157
EarI CTCTTC 7 cut(s) 865, 1806, 2264, 2556, 2846, 3060, 3168
EciI GGCGGA 4 cut(s) 157, 541, 600, 2352
Ecl136II GAGCTC 1 cut(s) 1285
Eco130I CCWWGG 1 cut(s) 1173
Eco24I GRGCYC 1 cut(s) 1287
Eco32I GATATC 1 cut(s) 1789
Eco47I GGWCC 3 cut(s) 2309, 2642, 3281
Eco53kI GAGCTC 1 cut(s) 1285
Eco57I CTGAAG 2 cut(s) 767, 3084
Eco88I CYCGRG 1 cut(s) 1192
EcoICRI GAGCTC 1 cut(s) 1285
EcoO109I RGGNCCY 1 cut(s) 2309
EcoRII CCWGG 2 cut(s) 2317, 3133
EcoRV GATATC 1 cut(s) 1789
EcoT14I CCWWGG 1 cut(s) 1173
EcoT22I ATGCAT 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 1287
EgeI GGCGCC 2 cut(s) 136, 769
EheI GGCGCC 2 cut(s) 136, 769
ErhI CCWWGG 1 cut(s) 1173
Esp3I CGTCTC 1 cut(s) 2558
FaeI CATG 8 cut(s) 177, 377, 1291, 2677, 2732, 2816, 3244, 3262
FaqI GGGAC 7 cut(s) 164, 545, 809, 1403, 1547, 1784, 2295
FatI CATG 8 cut(s) 173, 373, 1287, 2673, 2728, 2812, 3240, 3258
FauI CCCGC 3 cut(s) 135, 238, 408
FauNDI CATATG 1 cut(s) 2410
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 3 cut(s) 250, 2250, 2664
FokI GGATG 3 cut(s) 17, 151, 1187
FriOI GRGCYC 1 cut(s) 1287
FspBI CTAG 8 cut(s) 12, 884, 1232, 1308, 1803, 2775, 3128, 3149
GlaI GCGC 8 cut(s) 136, 185, 349, 408, 724, 738, 769, 897
GsaI CCCAGC 1 cut(s) 151
GsuI CTGGAG 3 cut(s) 551, 614, 965
HaeII RGCGCY 4 cut(s) 138, 410, 726, 771
HaeIII GGCC 7 cut(s) 488, 1125, 1918, 2322, 2377, 2743, 3329
HgaI GACGC 5 cut(s) 178, 1405, 2535, 2855, 3265
HhaI GCGC 8 cut(s) 137, 186, 350, 409, 725, 739, 770, 898
Hin1I GRCGYC 4 cut(s) 135, 189, 768, 2527
Hin1II CATG 8 cut(s) 177, 377, 1291, 2677, 2732, 2816, 3244, 3262
Hin6I GCGC 8 cut(s) 135, 184, 348, 407, 723, 737, 768, 896
HinP1I GCGC 8 cut(s) 135, 184, 348, 407, 723, 737, 768, 896
HincII GTYRAC 4 cut(s) 71, 251, 856, 2892
HindII GTYRAC 4 cut(s) 71, 251, 856, 2892
HindIII AAGCTT 3 cut(s) 779, 2276, 2381
HpaI GTTAAC 1 cut(s) 2892
Hpy188III TCNNGA 7 cut(s) 631, 826, 944, 1097, 1103, 2069, 2645
Hpy99I CGWCG 7 cut(s) 194, 731, 1210, 2529, 2849, 3281, 3301
HpyCH4III ACNGT 5 cut(s) 364, 1224, 1659, 1669, 2620
HpyCH4IV ACGT 2 cut(s) 1643, 2567
HpySE526I ACGT 2 cut(s) 1643, 2567
Hsp92I GRCGYC 4 cut(s) 135, 189, 768, 2527
Hsp92II CATG 8 cut(s) 177, 377, 1291, 2677, 2732, 2816, 3244, 3262
HspAI GCGC 8 cut(s) 135, 184, 348, 407, 723, 737, 768, 896
KasI GGCGCC 2 cut(s) 134, 767
KflI GGGWCCC 1 cut(s) 2309
KpnI GGTACC 1 cut(s) 1200
KroI GCCGGC 1 cut(s) 403
KroNI GCCGGC 1 cut(s) 405
Ksp22I TGATCA 1 cut(s) 27
KspAI GTTAAC 1 cut(s) 2892
LguI GCTCTTC 1 cut(s) 1806
Lsp1109I GCAGC 7 cut(s) 574, 925, 1303, 1497, 2191, 2978, 3111
LweI GCATC 4 cut(s) 624, 2146, 2614, 2761
MaeI CTAG 8 cut(s) 12, 884, 1232, 1308, 1803, 2775, 3128, 3149
MaeII ACGT 2 cut(s) 1643, 2567
MaeIII GTNAC 5 cut(s) 278, 1086, 1659, 2290, 2955
MbiI CCGCTC 3 cut(s) 130, 1050, 1251
MfeI CAATTG 3 cut(s) 1396, 1763, 2934
MflI RGATCY 5 cut(s) 87, 880, 1963, 2345, 3030
MhlI GDGCHC 5 cut(s) 677, 1222, 1287, 2517, 2556
MlsI TGGCCA 1 cut(s) 2743
MluNI TGGCCA 1 cut(s) 2743
Mly113I GGCGCC 2 cut(s) 135, 768
MlyI GAGTC 6 cut(s) 146, 219, 246, 650, 851, 3167
MmeI TCCRAC 7 cut(s) 720, 996, 1411, 3006, 3178, 3259, 3332
Mox20I TGGCCA 1 cut(s) 2743
Mph1103I ATGCAT 1 cut(s) 523
MreI CGCCGGCG 1 cut(s) 403
MroNI GCCGGC 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 931
MscI TGGCCA 1 cut(s) 2743
MseI TTAA 8 cut(s) 98, 1160, 1187, 2192, 2228, 2459, 2615, 2891
MslI CAYNNNNRTG 4 cut(s) 35, 539, 2721, 2817
Msp20I TGGCCA 1 cut(s) 2743
MspA1I CMGCKG 2 cut(s) 667, 2265
MspR9I CCNGG 8 cut(s) 215, 529, 1028, 1997, 2319, 2977, 3135, 3155
MunI CAATTG 3 cut(s) 1396, 1763, 2934
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 2 cut(s) 2319, 3135
MvnI CGCG 4 cut(s) 186, 245, 737, 3276
NaeI GCCGGC 1 cut(s) 405
NarI GGCGCC 2 cut(s) 135, 768
NciI CCSGG 6 cut(s) 215, 529, 1028, 1997, 2977, 3155
NdeI CATATG 1 cut(s) 2410
NgoMIV GCCGGC 1 cut(s) 403
NlaIII CATG 8 cut(s) 177, 377, 1291, 2677, 2732, 2816, 3244, 3262
NlaIV GGNNCC 8 cut(s) 136, 322, 629, 769, 1112, 1198, 2310, 2311
NmeAIII GCCGAG 3 cut(s) 111, 234, 1774
NmuCI GTSAC 3 cut(s) 278, 1659, 2955
NsiI ATGCAT 1 cut(s) 523
NspI RCATGY 1 cut(s) 2677
PciSI GCTCTTC 1 cut(s) 1806
PctI GAATGC 1 cut(s) 521
PdiI GCCGGC 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 931
PfeI GAWTC 6 cut(s) 468, 1235, 1543, 1687, 2065, 3167
PflMI CCANNNNNTGG 1 cut(s) 1718
PfoI TCCNGGA 1 cut(s) 213
PinAI ACCGGT 1 cut(s) 2886
PleI GAGTC 6 cut(s) 146, 218, 246, 650, 851, 3166
PluTI GGCGCC 2 cut(s) 138, 771
PpsI GAGTC 6 cut(s) 146, 218, 246, 650, 851, 3166
Ppu21I YACGTR 1 cut(s) 1644
PpuMI RGGWCCY 1 cut(s) 2309
PshBI ATTAAT 1 cut(s) 2192
Psp124BI GAGCTC 1 cut(s) 1287
Psp5II RGGWCCY 1 cut(s) 2309
Psp6I CCWGG 2 cut(s) 2317, 3133
PspFI CCCAGC 1 cut(s) 147
PspGI CCWGG 2 cut(s) 2317, 3133
PspN4I GGNNCC 8 cut(s) 136, 322, 629, 769, 1112, 1198, 2310, 2311
PspPI GGNCC 6 cut(s) 1123, 1916, 2309, 2375, 2642, 3281
PspPPI RGGWCCY 1 cut(s) 2309
PsuI RGATCY 5 cut(s) 87, 880, 1963, 2345, 3030
PvuII CAGCTG 1 cut(s) 667
RsaI GTAC 7 cut(s) 44, 1198, 1598, 1743, 2474, 2733, 2941
RsaNI GTAC 7 cut(s) 43, 1197, 1597, 1742, 2473, 2732, 2940
RseI CAYNNNNRTG 4 cut(s) 35, 539, 2721, 2817
Rsr2I CGGWCCG 1 cut(s) 3281
RsrII CGGWCCG 1 cut(s) 3281
SacI GAGCTC 1 cut(s) 1287
SalI GTCGAC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 1806
SaqAI TTAA 8 cut(s) 98, 1160, 1187, 2192, 2228, 2459, 2615, 2891
Sau96I GGNCC 6 cut(s) 1123, 1916, 2309, 2375, 2642, 3281
ScaI AGTACT 1 cut(s) 1743
SchI GAGTC 6 cut(s) 146, 219, 246, 650, 851, 3167
ScrFI CCNGG 8 cut(s) 215, 529, 1028, 1997, 2319, 2977, 3135, 3155
SduI GDGCHC 5 cut(s) 677, 1222, 1287, 2517, 2556
SfaNI GCATC 4 cut(s) 624, 2146, 2614, 2761
SfcI CTRYAG 1 cut(s) 2251
SfoI GGCGCC 2 cut(s) 136, 769
SgrAI CRCCGGYG 2 cut(s) 403, 599
SinI GGWCC 3 cut(s) 2309, 2642, 3281
SmiMI CAYNNNNRTG 4 cut(s) 35, 539, 2721, 2817
SmlI CTYRAG 4 cut(s) 638, 1566, 1745, 3143
SmoI CTYRAG 4 cut(s) 638, 1566, 1745, 3143
SspDI GGCGCC 2 cut(s) 134, 767
SspI AATATT 1 cut(s) 2005
SspMI CTAG 8 cut(s) 12, 884, 1232, 1308, 1803, 2775, 3128, 3149
SstI GAGCTC 1 cut(s) 1287
StyD4I CCNGG 8 cut(s) 213, 527, 1026, 1995, 2317, 2975, 3133, 3153
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 5 cut(s) 364, 1224, 1659, 1669, 2620
TaiI ACGT 2 cut(s) 1646, 2570
TaqI TCGA 8 cut(s) 250, 659, 935, 955, 1208, 2524, 2556, 2844
TaqII GACCGA 1 cut(s) 1007
TatI WGTACW 3 cut(s) 42, 1741, 2731
TauI GCSGC 8 cut(s) 56, 189, 606, 919, 2752, 2830, 2933, 3276
TfiI GAWTC 6 cut(s) 468, 1235, 1543, 1687, 2065, 3167
Tru1I TTAA 8 cut(s) 98, 1160, 1187, 2192, 2228, 2459, 2615, 2891
Tru9I TTAA 8 cut(s) 98, 1160, 1187, 2192, 2228, 2459, 2615, 2891
TscAI CASTG 4 cut(s) 873, 1227, 1461, 3057
TseFI GTSAC 3 cut(s) 278, 1659, 2955
TseI GCWGC 7 cut(s) 562, 913, 1291, 1510, 2204, 2966, 3124
Tsp45I GTSAC 3 cut(s) 278, 1659, 2955
TspDTI ATGAA 6 cut(s) 1875, 2126, 2484, 2574, 2801, 3247
TspGWI ACGGA 3 cut(s) 266, 1122, 2490
TspRI CASTG 4 cut(s) 873, 1227, 1461, 3057
Van91I CCANNNNNTGG 1 cut(s) 1718
VpaK11BI GGWCC 3 cut(s) 2309, 2642, 3281
VspI ATTAAT 1 cut(s) 2192
XapI RAATTY 9 cut(s) 1056, 1183, 1387, 1516, 1776, 1860, 2035, 2611, 2873
XceI RCATGY 1 cut(s) 2677
XmiI GTMKAC 3 cut(s) 250, 2250, 2664
XmnI GAANNNNTTC 1 cut(s) 931
XspI CTAG 8 cut(s) 12, 884, 1232, 1308, 1803, 2775, 3128, 3149
ZrmI AGTACT 1 cut(s) 1743
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.