MD15G1016500.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011850
Physical Location & Seq
Forward (+)
968934 .. 972371
3438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1016500.v1.1.491

Sequence Viewer

Length: 3438 bp
ATGGCGACGGCCACTTACTTCTTCCTTTTCCTCATAACAATTGTTATTCCTACAATTTATTTTCCCGCCACCTTAACGGCGGCTCAACGGCCGAGTCCAGAAACGTCACTCTCCGAGATCCAAGCGTTAACGGCGTTTAAGCGTAACCTCCACGACCCTCTTGGCGCTCTCGACGGCTGGGATTCGTCCACGCCGTCAGCCCCGTGCGACTGGCGCGGCGTCGTCTGTTACAGCAACCGAGTTCAGGAACTCCGCCTGCCTCGTCTCCACCTCGGCGGAAGAATCACTGACCACCTCGCCAGCCTCCGCGACCTCCGCAAGCTCAGCCTCTACAGCAACAACTTCAACGGCTCTATACCTCCCTCTCTCTCGCAATGCGCTCTGTTACGGGCCATGTACCTGCATGGCAACTCGCTCACCGGGACTCTTCCGCCGGCGATTTTGAACCTCACCAACCTCCAAATCCTTAATGTCGCCCATAATTTCCTCTCCGGAAAAATATCCGGCGACATTCCGGTGAGCCTCTGGTACCTTGACCTCTCCTCGAACACATTCTCCGGTGAAATTCCGGCGAACTTATCTGCCGAATCAAGCCTCCAGCTGATCAATCTCTCGTTCAATCGGTTCTCCGGCGGGGTTCCGGCGACCATCGGAGGTCTCAAGAATCTTGAGTATCTGTGGCTGGACTCGAACCAGCTGCACGGTACTCTGCCGTCGGCGATTTCCAATTGCTCGTCGCTAGTGCATTTGAGCGCGGACGACAACGAGCTGAGAGGTCTGATTCCGACGACGATCGGAGCGATGCCAAAGCTTCAGGTGTTGTCGCTGTCGCGGAACAACCTGTCAGGAGCGGTCCCCGCGTCGTTGTTGTGCAGTACTGCAAACAACGACTCGTCGTTGAGGATTGTGGAGCTCGGATTCAATGCACTCACGAGCATGGCTCAGCCGCCAGATATCTCAAAATGTTCGAGTGCAATGGGGGTTTTGGACTTAAAATCCAATCGGATAAGTGGCCCCTTCCCATCTTGGTTGACAAACGTGACAACAATTATGAGAATTCTAGATCTTTCTGGAAACATGTTTTCGGGTCAATTGCCGGCGAAGATTGGCAATCTTCTCCAGTTGGAGGAGTTTCGGGTGGCGAATAATTCGCTGAGTGGTGAAATTCCCGGTGAGATTGTGAAATGTAGTTTGTTACAGGTCCTTGATGTTGAAGGAAACAGATTTTTGGGTCAAGTCCCTTCTTTTTTAGGAGGAATGAGGAGCTTGAAGGTGTTGTCTTTGGGTGGGAATTTATTCTCTGGTGCCATTCCGTCAAATTTAGGGGCACTTTCAAAGCTCGAATCTTTGGATTTGAGCAACAATAATCTGACTGGGAAAGTTCCAGATGAGCTCACGATGCAGCTAAGCAACTTGACTAGTTTGAATCTGAGTAATAACAAGTTTTCCGGTGAGATTATTAATGTTGGAGAGCTGAAAAGCTTGCAGGTTTTGAATTTGAGCAATTGTGAGTTTTCTGGAAGTGTTCCTGCAAGCATTGGCGGTTTGATGAAGCTTGAAACACTGGATTTGAGTAAACAGAAGCTTTCTGGGGAGTTGCCGATTCAGATTTTCGGGTTGCCAAATTTACAAGTTGTTGCTGTGCAGGAAAATCACTTATCAGGAGATGTCCCTCAAGGTTTTAGTAGCTTGATTAGTTTGATCTATCTTAACCTATCTTCCAATGCTTTTACTGGTGATATTCCTACCACTTACGGATTTCTCAACTCTTTACTTGTTCTATCTTTGTCTGGAAATCGTATTTCGGGTACAATCCCACAAGAGCTTGGAGATTGTTTGAATCTTCAGGTACTTGAGCTTCGCTCTAATCAATTAGAAGGCAAAATTCCAGGTGATATATCTCACCTGTCCAATTTGAAAGAACTCAATGTAGGTCAGAATAAGCTGACCGGCAAAATCCCAGAGGAGATTTCAAAATGCTCTTCTCTGACTTCTCTGTTGTTAGATGCGAATCAACTTTCAGGTCACATACCAAACTCATTGCCTAAGTTATTGAACCTCACAGCGTTAGATCTTTCTTCTAACAATCTGAGTGGCACCATCCCTGAAAGTCTGTCGCTCCTCGTCCCTAACTTGAAATACCTGAATTTGTCATACAACAACCTTGCTGGAGAGATACCGAAGCCACTCGGTTCTCAGTTCAATGATCCTTCTGTGTTTGCAAGGAATGGAGATTTATGTGGGAAGCCATTGGATAGAGAATGTGCAGATGTGAGAAGGAGAAAAAGAAACAGGTTGACTTTATTAATTGCGGTGGCTGTGGGTGGAGCTTTCCTGCTGGCATTGTGTTGTTGTGGCTATATTTACAGCCTACTGCGATGGCGGAAAAAGCTCAGAGAAACAGTAATTGGGGTGCAGAAGAAGCGAACTAGCCCTCGAACAAGCTCAGGGGATAAAAGCAGCCGGGGAAGTGGCGCTAATGGAGGGCCAAAACTCGTGATGTTCAATAGCAAGATCACGTATGCAGAGGCATTAGAAGCTACAAGACAATTCGATGAGGAAAATGTGCTTAGCAGAGGAAGATATGGATTGGTATTCAAGGCTATGTTTCAAGATGGAATGGTGCTCGCAATTCGACGTCTTACTGATGGTTTTCTTGATGAAGGTGGCTTCCGCAAAGAAGCTGAAGATCTTGGCAAGGTGAAGCATCGAAACCTGACAGTTCTACGAGGCTATTATGCCGGTGCTCCTGACGTGAGGCTCCTTGTGTACGATTACATGCCGAATGGAAACTTAGGCGCCCTTCTACAAGAGGCATCTCACCAAGACGGGCATGTATTAAATTGGCCAATGCGGCACCTCATAGCACTCGGCATTGCTCGTGGATTAGCCTACCTGCACTCTGCCTCAATGGTACACGGCGATGTAAAGCCTCAAAATGTTCTTTTCGATGCTGATTTCGAAGCCCATTTATCCGAATTTGGTCTAGACCGGCTAACCATAGCCACACCAGCAAAAGCATCCTCGTCTTCAACCGCTATTGGCTCCCTAGGCTATGTGTCCCCCGAAGCTGCATTAACAGGGCAAGCAACGAAAGAAGCCGATGTGTATAGTTTTGGGATCGTATTGCTGGAGATATTGACCGGGAGGAATCCGGTGACATTTACTCAAGACGAAGACATTGTGAAGTGGGTGAAGAAGCAACTTCAGAGAGGTCAAGTTTCTGAGCTGCTGGAGCCCGGGCTGCTGGAACTAGACCCGGAATCTTCGGATTGGGAAGAGTTCTTGTTGGGAGTGAAAGTGGGGCTTCTTTGCACGGCTCCTGATCCGCTTGATCGGCCATCCATTGCCGACATTGTGTTCATGCTTGAAGGTTGCAGAGTCGGACCCGACATCCCGTCCTCAGCTGATCCGACCTCTCTGCCTTCACCAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1146

Amino Acids

123.49

Weight (kDa)

6.08

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 38 - 76 1.8e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 198 - 257 2.1e-06 Leucine rich repeat
LRR_14 PF23598 211 - 279 8.2e-06 Leucine-rich repeat region
LRR_14 PF23598 350 - 476 6.9e-11 Leucine-rich repeat region
LRR_8 PF13855 399 - 457 6.2e-07 Leucine rich repeat
LRR_8 PF13855 421 - 482 4.5e-07 Leucine rich repeat
LRR_14 PF23598 486 - 575 6.4e-09 Leucine-rich repeat region
LRR_8 PF13855 541 - 601 1.4e-07 Leucine rich repeat
LRR_14 PF23598 590 - 716 4.4e-06 Leucine-rich repeat region
LRR_8 PF13855 614 - 673 2.8e-08 Leucine rich repeat
Pkinase PF00069 853 - 1071 4.3e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 854 - 1070 2e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75640
fragaria_vesca FvH4_2g39080
malus_domestica MD08G1017600.v1.1 MD08G1017800.v1.1 MD15G1016500.v1.1
prunus_persica Prupe.1G370300_v2.0.a1
pyrus_communis pycom08g01540 pycom15g01450 pycom15g01460
rosa_chinensis RchiOBHm_Chr6g0302191
rosa_laevigata RLG00000011188
rosa_multiflora Rmu_co8058424.1_g000001
rosa_roxburghii Rroxscaffold_7G00166010
rosa_rugosa Rorug06G0313000
rosa_samantha Rh6AG425500 Rh6BG477000 Rh6CG438300 Rh6DG425300
rosa_wichuraiana Rw6G036850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2641
Acc36I ACCTGC 3 cut(s) 408, 1477, 2904
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 5 cut(s) 528, 1304, 2096, 2798, 2856
AccBSI CCGCTC 1 cut(s) 851
AccII CGCG 5 cut(s) 216, 309, 755, 832, 860
AccIII TCCGGA 1 cut(s) 491
AclWI GGATC 5 cut(s) 112, 2201, 3128, 3320, 3404
AcoI YGGCCR 4 cut(s) 9, 89, 2846, 3338
AcuI CTGAAG 4 cut(s) 797, 1829, 2706, 3191
AcyI GRCGYC 3 cut(s) 219, 2638, 2799
AfaI GTAC 8 cut(s) 398, 530, 706, 877, 1810, 1851, 2771, 2916
AfiI CCNNNNNNNGG 2 cut(s) 244, 1126
AflIII ACRYGT 1 cut(s) 1077
AhdI GACNNNNNGTC 1 cut(s) 3397
AhlI ACTAGT 1 cut(s) 1418
AjiI CACGTC 1 cut(s) 2755
AjnI CCWGG 1 cut(s) 1888
AjuI GAANNNNNNNTTGG 2 cut(s) 2391, 2423
AloI GAACNNNNNNTCC 2 cut(s) 539, 571
Alw21I GWGCWC 4 cut(s) 915, 1395, 2628, 2749
Alw26I GTCTC 2 cut(s) 269, 662
AlwI GGATC 5 cut(s) 112, 2201, 3128, 3320, 3404
Ama87I CYCGRG 1 cut(s) 3239
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 7 cut(s) 9, 89, 390, 1012, 2486, 2846, 3338
ApeKI GCWGC 6 cut(s) 697, 1402, 2460, 3071, 3229, 3244
AseI ATTAAT 2 cut(s) 1461, 2306
Asp700I GAANNNNTTC 2 cut(s) 551, 1295
Asp718I GGTACC 1 cut(s) 528
AspA2I CCTAGG 1 cut(s) 3049
AspLEI GCGC 6 cut(s) 167, 216, 380, 755, 2477, 2801
AspS9I GGNCC 6 cut(s) 390, 853, 1013, 1201, 2486, 3386
AsuC2I CCSGG 7 cut(s) 421, 1170, 2465, 3145, 3240, 3241, 3260
AsuII TTCGAA 1 cut(s) 2961
AvaI CYCGRG 1 cut(s) 3239
AvaII GGWCC 3 cut(s) 853, 1201, 3386
AvrII CCTAGG 1 cut(s) 3049
BaeGI GKGCMC 1 cut(s) 1330
BalI TGGCCA 1 cut(s) 2848
BanI GGYRCC 5 cut(s) 528, 1304, 2096, 2798, 2856
BanII GRGCYC 3 cut(s) 915, 1395, 3240
BarI GAAGNNNNNNTAC 2 cut(s) 1842, 1874
BauI CACGAG 3 cut(s) 931, 2495, 2880
BbsI GAAGAC 2 cut(s) 3021, 3183
Bbv12I GWGCWC 4 cut(s) 915, 1395, 2628, 2749
BbvCI CCTCAGC 1 cut(s) 3403
BbvI GCAGC 6 cut(s) 684, 1414, 2472, 3058, 3216, 3231
BccI CCATC 7 cut(s) 656, 1030, 2108, 2373, 2609, 2642, 3349
BcgI CGANNNNNNTGC 4 cut(s) 679, 713, 3403, 3437
BciT130I CCWGG 1 cut(s) 1890
BclI TGATCA 1 cut(s) 603
BcnI CCSGG 7 cut(s) 421, 1170, 2465, 3145, 3240, 3241, 3260
BcoDI GTCTC 2 cut(s) 269, 662
BcuI ACTAGT 1 cut(s) 1418
BfaI CTAG 7 cut(s) 740, 1061, 1419, 2430, 2987, 3050, 3254
BfmI CTRYAG 1 cut(s) 331
BfoI RGCGCY 3 cut(s) 168, 2478, 2802
BfuAI ACCTGC 3 cut(s) 408, 1477, 2904
BglI GCCNNNNNGGC 1 cut(s) 2854
BglII AGATCT 3 cut(s) 1063, 2071, 2689
BlnI CCTAGG 1 cut(s) 3049
BlpI GCTNAGC 4 cut(s) 323, 942, 1406, 2570
BmcAI AGTACT 1 cut(s) 877
Bme1390I CCNGG 8 cut(s) 421, 1170, 1890, 2465, 3145, 3240, 3241, 3260
Bme18I GGWCC 3 cut(s) 853, 1201, 3386
BmeRI GACNNNNNGTC 1 cut(s) 3397
BmeT110I CYCGRG 1 cut(s) 3239
BmgBI CACGTC 1 cut(s) 2755
BmgT120I GGNCC 6 cut(s) 390, 853, 1013, 1201, 2486, 3386
BmrFI CCNGG 8 cut(s) 421, 1170, 1890, 2465, 3145, 3240, 3241, 3260
BmrI ACTGGG 1 cut(s) 1383
BmsI GCATC 7 cut(s) 792, 1389, 1996, 2716, 2825, 2941, 3029
BmuI ACTGGG 1 cut(s) 1383
BpiI GAAGAC 2 cut(s) 3021, 3183
BplI GAGNNNNNCTC 4 cut(s) 925, 957, 1847, 1879
BpmI CTGGAG 5 cut(s) 581, 1103, 2190, 3152, 3254
Bpu10I CCTNAGC 2 cut(s) 2446, 3403
Bpu1102I GCTNAGC 4 cut(s) 323, 942, 1406, 2570
Bpu14I TTCGAA 1 cut(s) 2961
BpuEI CTTGAG 5 cut(s) 644, 689, 1659, 1874, 3153
BpuMI CCSGG 7 cut(s) 421, 1170, 2465, 3145, 3240, 3241, 3260
BsaAI YACGTR 1 cut(s) 2520
BsaBI GATNNNNATC 1 cut(s) 2010
BsaHI GRCGYC 3 cut(s) 219, 2638, 2799
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 4 cut(s) 271, 2464, 3049, 3239
BsaWI WCCGGW 5 cut(s) 491, 514, 557, 1448, 3154
BsaXI ACNNNNNCTCC 4 cut(s) 539, 569, 3285, 3315
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 1126
Bse118I RCCGGY 5 cut(s) 433, 1096, 1949, 2741, 2991
Bse1I ACTGG 5 cut(s) 215, 1120, 1378, 1569, 1738
Bse3DI GCAATG 5 cut(s) 380, 981, 2039, 2874, 3345
Bse8I GATNNNNATC 1 cut(s) 2010
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 1 cut(s) 1890
BseDI CCNNGG 4 cut(s) 271, 2464, 3049, 3239
BseGI GGATG 4 cut(s) 2100, 3020, 3341, 3393
BseJI GATNNNNATC 1 cut(s) 2010
BseLI CCNNNNNNNGG 2 cut(s) 244, 1126
BseMI GCAATG 5 cut(s) 380, 981, 2039, 2874, 3345
BseNI ACTGG 5 cut(s) 215, 1120, 1378, 1569, 1738
BseRI GAGGAG 5 cut(s) 532, 1142, 1276, 1979, 2111
BseSI GKGCMC 1 cut(s) 1330
BseX3I CGGCCG 1 cut(s) 89
BseXI GCAGC 6 cut(s) 684, 1414, 2472, 3058, 3216, 3231
BseYI CCCAGC 1 cut(s) 177
BsgI GTGCAG 6 cut(s) 683, 892, 1664, 2286, 2435, 2882
Bsh1236I CGCG 5 cut(s) 216, 309, 755, 832, 860
Bsh1285I CGRYCG 2 cut(s) 92, 795
BshFI GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3340
BshNI GGYRCC 5 cut(s) 528, 1304, 2096, 2798, 2856
BsiEI CGRYCG 2 cut(s) 92, 795
BsiHKAI GWGCWC 4 cut(s) 915, 1395, 2628, 2749
BsiHKCI CYCGRG 1 cut(s) 3239
BslFI GGGAC 6 cut(s) 436, 839, 1223, 1655, 2111, 3046
BslI CCNNNNNNNGG 2 cut(s) 244, 1126
BsmAI GTCTC 2 cut(s) 269, 662
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 6 cut(s) 436, 839, 1223, 1655, 2111, 3046
BsnI GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3340
Bso31I GGTCTC 1 cut(s) 662
BsoBI CYCGRG 1 cut(s) 3239
Bsp119I TTCGAA 1 cut(s) 2961
Bsp1286I GDGCHC 6 cut(s) 915, 1330, 1395, 2628, 2749, 3240
Bsp13I TCCGGA 1 cut(s) 491
Bsp1720I GCTNAGC 4 cut(s) 323, 942, 1406, 2570
BspANI GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3340
BspEI TCCGGA 1 cut(s) 491
BspFNI CGCG 5 cut(s) 216, 309, 755, 832, 860
BspMI ACCTGC 3 cut(s) 408, 1477, 2904
BspPI GGATC 5 cut(s) 112, 2201, 3128, 3320, 3404
BspQI GCTCTTC 1 cut(s) 1987
BspT104I TTCGAA 1 cut(s) 2961
BspT107I GGYRCC 5 cut(s) 528, 1304, 2096, 2798, 2856
BspTNI GGTCTC 1 cut(s) 662
BsrBI CCGCTC 1 cut(s) 851
BsrDI GCAATG 5 cut(s) 380, 981, 2039, 2874, 3345
BsrFI RCCGGY 5 cut(s) 433, 1096, 1949, 2741, 2991
BsrI ACTGG 5 cut(s) 215, 1120, 1378, 1569, 1738
BssAI RCCGGY 5 cut(s) 433, 1096, 1949, 2741, 2991
BssECI CCNNGG 4 cut(s) 271, 2464, 3049, 3239
BssNI GRCGYC 3 cut(s) 219, 2638, 2799
BssSI CACGAG 3 cut(s) 931, 2495, 2880
BssT1I CCWWGG 1 cut(s) 3049
Bst2BI CACGAG 3 cut(s) 931, 2495, 2880
Bst2UI CCWGG 1 cut(s) 1890
Bst4CI ACNGT 3 cut(s) 704, 2404, 2722
Bst6I CTCTTC 3 cut(s) 432, 1987, 3273
BstACI GRCGYC 3 cut(s) 219, 2638, 2799
BstBAI YACGTR 1 cut(s) 2520
BstBI TTCGAA 1 cut(s) 2961
BstF5I GGATG 4 cut(s) 2100, 3020, 3341, 3393
BstFNI CGCG 5 cut(s) 216, 309, 755, 832, 860
BstH2I RGCGCY 3 cut(s) 168, 2478, 2802
BstHHI GCGC 6 cut(s) 167, 216, 380, 755, 2477, 2801
BstMAI GTCTC 2 cut(s) 269, 662
BstMCI CGRYCG 2 cut(s) 92, 795
BstNI CCWGG 1 cut(s) 1890
BstNSI RCATGY 3 cut(s) 1081, 2782, 2837
BstSCI CCNGG 8 cut(s) 419, 1168, 1888, 2463, 3143, 3238, 3239, 3258
BstSFI CTRYAG 1 cut(s) 331
BstSLI GKGCMC 1 cut(s) 1330
BstUI CGCG 5 cut(s) 216, 309, 755, 832, 860
BstV1I GCAGC 6 cut(s) 684, 1414, 2472, 3058, 3216, 3231
BstV2I GAAGAC 2 cut(s) 3021, 3183
BstX2I RGATCY 4 cut(s) 117, 1063, 2071, 2689
BstYI RGATCY 4 cut(s) 117, 1063, 2071, 2689
BstZI CGGCCG 1 cut(s) 89
BsuRI GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3340
BtgZI GCGATG 3 cut(s) 815, 2392, 2937
BtrI CACGTC 1 cut(s) 2755
BtsCI GGATG 4 cut(s) 2100, 3020, 3341, 3393
BtsIMutI CAGTG 2 cut(s) 285, 1562
BveI ACCTGC 3 cut(s) 408, 1477, 2904
CfoI GCGC 6 cut(s) 167, 216, 380, 755, 2477, 2801
Cfr10I RCCGGY 5 cut(s) 433, 1096, 1949, 2741, 2991
Cfr13I GGNCC 6 cut(s) 390, 853, 1013, 1201, 2486, 3386
Cfr9I CCCGGG 1 cut(s) 3239
CseI GACGC 2 cut(s) 208, 849
Csp6I GTAC 8 cut(s) 397, 529, 705, 876, 1809, 1850, 2770, 2915
CspCI CAANNNNNGTGG 2 cut(s) 2074, 2109
CviAII CATG 7 cut(s) 394, 404, 937, 1078, 2779, 2834, 3364
CviQI GTAC 8 cut(s) 397, 529, 705, 876, 1809, 1850, 2770, 2915
DinI GGCGCC 1 cut(s) 2800
DriI GACNNNNNGTC 1 cut(s) 3397
EaeI YGGCCR 4 cut(s) 9, 89, 2846, 3338
EagI CGGCCG 1 cut(s) 89
Eam1104I CTCTTC 3 cut(s) 432, 1987, 3273
Eam1105I GACNNNNNGTC 1 cut(s) 3397
EarI CTCTTC 3 cut(s) 432, 1987, 3273
EciI GGCGGA 4 cut(s) 242, 291, 420, 2398
Ecl136II GAGCTC 2 cut(s) 913, 1393
EclXI CGGCCG 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 3049
Eco24I GRGCYC 3 cut(s) 915, 1395, 3240
Eco31I GGTCTC 1 cut(s) 662
Eco32I GATATC 1 cut(s) 955
Eco47I GGWCC 3 cut(s) 853, 1201, 3386
Eco52I CGGCCG 1 cut(s) 89
Eco53kI GAGCTC 2 cut(s) 913, 1393
Eco57I CTGAAG 4 cut(s) 797, 1829, 2706, 3191
Eco88I CYCGRG 1 cut(s) 3239
EcoICRI GAGCTC 2 cut(s) 913, 1393
EcoO109I RGGNCCY 1 cut(s) 1201
EcoRI GAATTC 1 cut(s) 1056
EcoRII CCWGG 1 cut(s) 1888
EcoRV GATATC 1 cut(s) 955
EcoT14I CCWWGG 1 cut(s) 3049
EcoT38I GRGCYC 3 cut(s) 915, 1395, 3240
EgeI GGCGCC 1 cut(s) 2800
EheI GGCGCC 1 cut(s) 2800
ErhI CCWWGG 1 cut(s) 3049
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 7 cut(s) 397, 407, 940, 1081, 2782, 2837, 3367
FalI AAGNNNNNCTT 2 cut(s) 3291, 3323
FaqI GGGAC 6 cut(s) 436, 839, 1223, 1655, 2111, 3046
FatI CATG 7 cut(s) 393, 403, 936, 1077, 2778, 2833, 3363
FauI CCCGC 3 cut(s) 73, 626, 865
FbaI TGATCA 1 cut(s) 603
FokI GGATG 4 cut(s) 2087, 3007, 3328, 3380
FriOI GRGCYC 3 cut(s) 915, 1395, 3240
FspBI CTAG 7 cut(s) 740, 1061, 1419, 2430, 2987, 3050, 3254
GlaI GCGC 6 cut(s) 166, 215, 379, 754, 2476, 2800
GsaI CCCAGC 1 cut(s) 181
GsuI CTGGAG 5 cut(s) 581, 1103, 2190, 3152, 3254
HaeII RGCGCY 3 cut(s) 168, 2478, 2802
HaeIII GGCC 7 cut(s) 11, 91, 392, 1014, 2488, 2848, 3340
HgaI GACGC 2 cut(s) 208, 849
HhaI GCGC 6 cut(s) 167, 216, 380, 755, 2477, 2801
Hin1I GRCGYC 3 cut(s) 219, 2638, 2799
Hin1II CATG 7 cut(s) 397, 407, 940, 1081, 2782, 2837, 3367
Hin6I GCGC 6 cut(s) 165, 214, 378, 753, 2475, 2799
HinP1I GCGC 6 cut(s) 165, 214, 378, 753, 2475, 2799
HincII GTYRAC 3 cut(s) 129, 1032, 2298
HindII GTYRAC 3 cut(s) 129, 1032, 2298
HindIII AAGCTT 4 cut(s) 809, 1480, 1553, 1583
HpaI GTTAAC 1 cut(s) 129
Hpy166II GTNNAC 7 cut(s) 129, 189, 1032, 1577, 2298, 2770, 2917
Hpy8I GTNNAC 7 cut(s) 129, 189, 1032, 1577, 2298, 2770, 2917
HpyCH4III ACNGT 3 cut(s) 704, 2404, 2722
HpyCH4IV ACGT 5 cut(s) 104, 1038, 2519, 2638, 2754
HpySE526I ACGT 5 cut(s) 104, 1038, 2519, 2638, 2754
Hsp92I GRCGYC 3 cut(s) 219, 2638, 2799
Hsp92II CATG 7 cut(s) 397, 407, 940, 1081, 2782, 2837, 3367
HspAI GCGC 6 cut(s) 165, 214, 378, 753, 2475, 2799
KasI GGCGCC 1 cut(s) 2798
Kpn2I TCCGGA 1 cut(s) 491
KpnI GGTACC 1 cut(s) 532
KroI GCCGGC 2 cut(s) 433, 1096
KroNI GCCGGC 2 cut(s) 435, 1098
Ksp22I TGATCA 1 cut(s) 603
KspAI GTTAAC 1 cut(s) 129
LguI GCTCTTC 1 cut(s) 1987
Lsp1109I GCAGC 6 cut(s) 684, 1414, 2472, 3058, 3216, 3231
LweI GCATC 7 cut(s) 792, 1389, 1996, 2716, 2825, 2941, 3029
MaeI CTAG 7 cut(s) 740, 1061, 1419, 2430, 2987, 3050, 3254
MaeII ACGT 5 cut(s) 104, 1038, 2519, 2638, 2754
MaeIII GTNAC 8 cut(s) 105, 143, 227, 384, 1039, 1194, 2024, 3157
MbiI CCGCTC 1 cut(s) 851
MfeI CAATTG 4 cut(s) 39, 727, 1091, 1504
MflI RGATCY 4 cut(s) 117, 1063, 2071, 2689
MhlI GDGCHC 6 cut(s) 915, 1330, 1395, 2628, 2749, 3240
MlsI TGGCCA 1 cut(s) 2848
MluNI TGGCCA 1 cut(s) 2848
Mly113I GGCGCC 1 cut(s) 2799
MlyI GAGTC 5 cut(s) 103, 418, 680, 884, 3390
MmeI TCCRAC 5 cut(s) 809, 1104, 1447, 3364, 3437
Mox20I TGGCCA 1 cut(s) 2848
MreI CGCCGGCG 1 cut(s) 433
MroI TCCGGA 1 cut(s) 491
MroNI GCCGGC 2 cut(s) 433, 1096
MroXI GAANNNNTTC 2 cut(s) 551, 1295
MscI TGGCCA 1 cut(s) 2848
MslI CAYNNNNRTG 3 cut(s) 515, 935, 2922
Msp20I TGGCCA 1 cut(s) 2848
MspA1I CMGCKG 3 cut(s) 601, 697, 3407
MspR9I CCNGG 8 cut(s) 421, 1170, 1890, 2465, 3145, 3240, 3241, 3260
MunI CAATTG 4 cut(s) 39, 727, 1091, 1504
MvaI CCWGG 1 cut(s) 1890
MvnI CGCG 5 cut(s) 216, 309, 755, 832, 860
NaeI GCCGGC 2 cut(s) 435, 1098
NarI GGCGCC 1 cut(s) 2799
NciI CCSGG 7 cut(s) 421, 1170, 2465, 3145, 3240, 3241, 3260
NgoMIV GCCGGC 2 cut(s) 433, 1096
NlaIII CATG 7 cut(s) 397, 407, 940, 1081, 2782, 2837, 3367
NmeAIII GCCGAG 3 cut(s) 117, 252, 2850
NmuCI GTSAC 4 cut(s) 105, 1039, 2024, 3157
NspI RCATGY 3 cut(s) 1081, 2782, 2837
NspV TTCGAA 1 cut(s) 2961
PciI ACATGT 1 cut(s) 1077
PciSI GCTCTTC 1 cut(s) 1987
PcsI WCGNNNNNNNCGW 2 cut(s) 191, 797
PdiI GCCGGC 2 cut(s) 435, 1098
PdmI GAANNNNTTC 2 cut(s) 551, 1295
Ple19I CGATCG 1 cut(s) 795
PleI GAGTC 5 cut(s) 102, 418, 680, 884, 3389
PluTI GGCGCC 1 cut(s) 2802
PpsI GAGTC 5 cut(s) 102, 418, 680, 884, 3389
Ppu21I YACGTR 1 cut(s) 2520
PpuMI RGGWCCY 1 cut(s) 1201
PscI ACATGT 1 cut(s) 1077
PshBI ATTAAT 2 cut(s) 1461, 2306
Psp124BI GAGCTC 2 cut(s) 915, 1395
Psp5II RGGWCCY 1 cut(s) 1201
Psp6I CCWGG 1 cut(s) 1888
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 1 cut(s) 1888
PspPI GGNCC 6 cut(s) 390, 853, 1013, 1201, 2486, 3386
PspPPI RGGWCCY 1 cut(s) 1201
PsuI RGATCY 4 cut(s) 117, 1063, 2071, 2689
PvuI CGATCG 1 cut(s) 795
PvuII CAGCTG 3 cut(s) 601, 697, 3407
RsaI GTAC 8 cut(s) 398, 530, 706, 877, 1810, 1851, 2771, 2916
RsaNI GTAC 8 cut(s) 397, 529, 705, 876, 1809, 1850, 2770, 2915
RseI CAYNNNNRTG 3 cut(s) 515, 935, 2922
SacI GAGCTC 2 cut(s) 915, 1395
SapI GCTCTTC 1 cut(s) 1987
Sau96I GGNCC 6 cut(s) 390, 853, 1013, 1201, 2486, 3386
ScaI AGTACT 1 cut(s) 877
SchI GAGTC 5 cut(s) 103, 418, 680, 884, 3390
ScrFI CCNGG 8 cut(s) 421, 1170, 1890, 2465, 3145, 3240, 3241, 3260
SduI GDGCHC 6 cut(s) 915, 1330, 1395, 2628, 2749, 3240
SfaNI GCATC 7 cut(s) 792, 1389, 1996, 2716, 2825, 2941, 3029
SfcI CTRYAG 1 cut(s) 331
SfoI GGCGCC 1 cut(s) 2800
SfuI TTCGAA 1 cut(s) 2961
SgrAI CRCCGGYG 1 cut(s) 433
SinI GGWCC 3 cut(s) 853, 1201, 3386
SmaI CCCGGG 1 cut(s) 3241
SmiMI CAYNNNNRTG 3 cut(s) 515, 935, 2922
SmlI CTYRAG 5 cut(s) 659, 668, 1674, 1853, 3168
SmoI CTYRAG 5 cut(s) 659, 668, 1674, 1853, 3168
SpeI ACTAGT 1 cut(s) 1418
SrfI GCCCGGGC 1 cut(s) 3241
SspDI GGCGCC 1 cut(s) 2798
SspMI CTAG 7 cut(s) 740, 1061, 1419, 2430, 2987, 3050, 3254
SstI GAGCTC 2 cut(s) 915, 1395
StyD4I CCNGG 8 cut(s) 419, 1168, 1888, 2463, 3143, 3238, 3239, 3258
StyI CCWWGG 1 cut(s) 3049
TaaI ACNGT 3 cut(s) 704, 2404, 2722
TaiI ACGT 5 cut(s) 107, 1041, 2522, 2641, 2757
TatI WGTACW 1 cut(s) 875
TauI GCSGC 4 cut(s) 83, 219, 949, 2857
TscAI CASTG 2 cut(s) 292, 1569
TseFI GTSAC 4 cut(s) 105, 1039, 2024, 3157
TseI GCWGC 6 cut(s) 697, 1402, 2460, 3071, 3229, 3244
Tsp45I GTSAC 4 cut(s) 105, 1039, 2024, 3157
TspDTI ATGAA 3 cut(s) 1565, 2676, 3352
TspGWI ACGGA 2 cut(s) 1302, 1770
TspMI CCCGGG 1 cut(s) 3239
TspRI CASTG 2 cut(s) 292, 1569
VpaK11BI GGWCC 3 cut(s) 853, 1201, 3386
VspI ATTAAT 2 cut(s) 1461, 2306
XbaI TCTAGA 2 cut(s) 1060, 2986
XceI RCATGY 3 cut(s) 1081, 2782, 2837
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XmaI CCCGGG 1 cut(s) 3239
XmaJI CCTAGG 1 cut(s) 3049
XmnI GAANNNNTTC 2 cut(s) 551, 1295
XspI CTAG 7 cut(s) 740, 1061, 1419, 2430, 2987, 3050, 3254
ZraI GACGTC 1 cut(s) 2639
ZrmI AGTACT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.