FvH4_2g39730

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010704
Physical Location & Seq
Reverse (-)
28451578 .. 28453119
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39730.t1

Sequence Viewer

Length: 1542 bp
ATGGACCAAGGCGGCCCGGCCCACGCCGCCGGCGCCGGAGGCCATGTCGGAGTCCGGATCCACCACACACGACGCCTCCCGGATTTCCTCCAGAGCGTGAATCTCAAGTACGTCAAACTCGGCTACCACTATCTCATCTCCAATCTCTTAACCCTCTGCTTCATCCCCTTAATCATCGTCACGTTAATCGAGGCCTCCCAAATGGACCTCCGCGACCTCCGCCACCTCTGGCTCCACCTCCAGTACAACCTCGTCAGCGTCATCATCTGCTCCGCCGTCCTCGTCTTCGGGTTGACCGTTTACATCATGACCCGGCCCAGGCCCATTTACCTAGTGGACTACTCCTGCTACCGCCCCCCTGACCACCTCAAGGCGCCCTTCCGCCGCTTCATGGACCACTCCCGCCAAACCAACGACTTCGACGACTCGTCGTTGGAGTTCCAGCGCAAGATTCTCCAACGCTCCGGCCTCGGAGAAGAGACCTACGTCCCGGAGGCGATGCACCACATCCCTCCCCGGCCGTCGATGGCGGCGGCGAGGGAGGAGGCCGAGGAGGTCATGTATGGAGCGCTGGATAATCTGTTTGCAAACACTCATGTAAAACCGAAGGACATTGGGATTCTCGTTGTGAATTGCAGCTTGTTTAATCCGACGCCGTCGCTTTCGGCGATGATTGTGAACAGGTATAAGCTCCGGGGGAATATTCGGAGTTTCAATTTGGGAGGGATGGGGTGCAGCGCTGGAGTTATAGCTGTTGATCTTGCTAAGGACTTGTTGCAGATTCATAGGAATACTTGTGCTGTAGTGGTCAGTACTGAGAACATTACGCAGAATTGGTATTTTGGGAATAAGAAGTCAATGTTGATACCGAATTGCTTGTTTCGAGTTGGGGGATCCGCGGTTTTGCTGTCGAATAAGGCTGTGGATAGGAGGCGGGCGAAGTACAGGCTTGTTCATGTTGTGAGGACTCATAAGGGCGCGGATGACAAGGCGTTTCGGTGTGTTTATCAGGAGCAGGATGATAAGGGGAAGACTGGTGTGTCTCTGTCGAAAGATTTGATGGCCATTGCTGGTGGGGCGCTTAAGACGAATATAACTACTCTGGGGCCTATTGTGCTTCCGATAAGCGAGCAGCTGCTTTTCTTCTCTTCTCTTGTGGTTAAGAAGCTGTTGAATGGGGATGTCAAGCCGTATATACCGGATTTCAAGCTGGCCTTTGATCATTTCTGTATACATGCTGGTGGAAGGGCTGTGATTGATGAGCTGGAGAAGAACTTGCAGTTGTTGCCGATACATGTGGAGGCTTCTCGGATGACTCTACACCGGTTTGGGAATACTTCGTCGAGCTCCATTTGGTATGAGTTGGCCTATACGGAGGCCAAAGGGAGGATGCGCAAGGGCAACCGAGTGTGGCAGATTGCCTTTGGGAGCGGTTTCAAATGTAACAGTGCAGTCTGGCAGGCTCTGCGCAATGTGCAGCCATCTTGTAATGGTCCTTGGGAGGACAGCATTGACAAGTATCCCATGAAAGTAGTTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

514

Amino Acids

57.57

Weight (kDa)

9.35

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 101 - 390 8e-146 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 407 - 487 3.5e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 409 - 482 8.9e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1039
Acc16I TGCGCA 2 cut(s) 1394, 1469
AccB1I GGYRCC 2 cut(s) 32, 373
AccBSI CCGCTC 1 cut(s) 1431
AccI GTMKAC 1 cut(s) 1231
AccII CGCG 3 cut(s) 213, 899, 980
AccIII TCCGGA 1 cut(s) 54
AclWI GGATC 4 cut(s) 52, 65, 888, 901
AcoI YGGCCR 2 cut(s) 518, 1062
AcyI GRCGYC 4 cut(s) 33, 73, 374, 653
AfaI GTAC 4 cut(s) 110, 245, 814, 944
AfeI AGCGCT 2 cut(s) 570, 739
AfiI CCNNNNNNNGG 4 cut(s) 318, 370, 391, 1386
AflII CTTAAG 1 cut(s) 1082
AflIII ACRYGT 1 cut(s) 1294
AgeI ACCGGT 1 cut(s) 1323
AgsI TTSAA 4 cut(s) 715, 1174, 1207, 1438
AhdI GACNNNNNGTC 1 cut(s) 427
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 8 cut(s) 639, 691, 752, 1135, 1168, 1210, 1264, 1347
AluI AGCT 8 cut(s) 639, 691, 752, 1135, 1168, 1210, 1264, 1347
Alw21I GWGCWC 1 cut(s) 1349
Alw26I GTCTC 2 cut(s) 473, 1047
AlwI GGATC 4 cut(s) 52, 65, 888, 901
AlwNI CAGNNNCTG 1 cut(s) 1465
Aor13HI TCCGGA 1 cut(s) 54
Aor51HI AGCGCT 2 cut(s) 570, 739
ApeKI GCWGC 5 cut(s) 636, 735, 1132, 1135, 1477
AsiGI ACCGGT 1 cut(s) 1323
AspLEI GCGC 9 cut(s) 35, 376, 447, 571, 740, 980, 1081, 1395, 1470
AspS9I GGNCC 9 cut(s) 4, 14, 19, 205, 315, 321, 394, 1106, 1493
AsuC2I CCSGG 6 cut(s) 17, 80, 313, 491, 517, 695
AvaII GGWCC 4 cut(s) 4, 205, 394, 1493
BalI TGGCCA 1 cut(s) 1064
BamHI GGATCC 2 cut(s) 57, 893
BanI GGYRCC 2 cut(s) 32, 373
BanII GRGCYC 1 cut(s) 1349
BbsI GAAGAC 2 cut(s) 277, 1037
Bbv12I GWGCWC 1 cut(s) 1349
BbvI GCAGC 5 cut(s) 648, 747, 1122, 1144, 1489
BccI CCATC 4 cut(s) 520, 721, 1054, 1489
BceAI ACGGC 4 cut(s) 260, 505, 640, 1174
BciT130I CCWGG 1 cut(s) 319
BciVI GTATCC 1 cut(s) 1530
BclI TGATCA 1 cut(s) 1219
BcnI CCSGG 6 cut(s) 17, 80, 313, 491, 517, 695
BcoDI GTCTC 2 cut(s) 473, 1047
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 801
BfoI RGCGCY 5 cut(s) 36, 377, 572, 741, 1082
BfrI CTTAAG 1 cut(s) 1082
BfuI GTATCC 1 cut(s) 1530
BmcAI AGTACT 1 cut(s) 814
Bme1390I CCNGG 7 cut(s) 17, 80, 313, 319, 491, 517, 695
Bme18I GGWCC 4 cut(s) 4, 205, 394, 1493
BmeRI GACNNNNNGTC 1 cut(s) 427
BmgT120I GGNCC 9 cut(s) 4, 14, 19, 205, 315, 321, 394, 1106, 1493
BmiI GGNNCC 6 cut(s) 34, 59, 233, 375, 895, 1107
BmrFI CCNGG 7 cut(s) 17, 80, 313, 319, 491, 517, 695
BmsI GCATC 2 cut(s) 489, 1380
BoxI GACNNNNGTC 1 cut(s) 485
BpiI GAAGAC 2 cut(s) 277, 1037
BpmI CTGGAG 4 cut(s) 74, 224, 762, 1286
Bpu10I CCTNAGC 1 cut(s) 765
BpuEI CTTGAG 2 cut(s) 89, 353
BpuMI CCSGG 6 cut(s) 17, 80, 313, 491, 517, 695
BsaHI GRCGYC 4 cut(s) 33, 73, 374, 653
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 8 cut(s) 7, 317, 469, 515, 549, 694, 897, 1496
BsaWI WCCGGW 3 cut(s) 54, 1198, 1323
BsaXI ACNNNNNCTCC 6 cut(s) 30, 60, 60, 90, 545, 575
Bsc4I CCNNNNNNNGG 4 cut(s) 318, 370, 391, 1386
Bse118I RCCGGY 2 cut(s) 29, 1323
Bse1I ACTGG 2 cut(s) 241, 1039
Bse3DI GCAATG 2 cut(s) 1065, 1477
BseAI TCCGGA 1 cut(s) 54
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 8 cut(s) 7, 317, 469, 515, 549, 694, 897, 1496
BseGI GGATG 8 cut(s) 162, 507, 732, 988, 1024, 1186, 1317, 1395
BseLI CCNNNNNNNGG 4 cut(s) 318, 370, 391, 1386
BseMI GCAATG 2 cut(s) 1065, 1477
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 2 cut(s) 241, 1039
BseRI GAGGAG 2 cut(s) 557, 566
BseX3I CGGCCG 1 cut(s) 518
BseXI GCAGC 5 cut(s) 648, 747, 1122, 1144, 1489
BsgI GTGCAG 3 cut(s) 754, 1470, 1496
Bsh1236I CGCG 3 cut(s) 213, 899, 980
Bsh1285I CGRYCG 1 cut(s) 521
BshNI GGYRCC 2 cut(s) 32, 373
BshTI ACCGGT 1 cut(s) 1323
BsiEI CGRYCG 1 cut(s) 521
BsiHKAI GWGCWC 1 cut(s) 1349
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 4 cut(s) 318, 370, 391, 1386
BsmAI GTCTC 2 cut(s) 473, 1047
BsmFI GGGAC 1 cut(s) 473
Bso31I GGTCTC 1 cut(s) 473
Bsp1286I GDGCHC 1 cut(s) 1349
Bsp13I TCCGGA 1 cut(s) 54
Bsp143I GATC 4 cut(s) 57, 757, 893, 1219
BspCNI CTCAG 1 cut(s) 808
BspEI TCCGGA 1 cut(s) 54
BspFNI CGCG 3 cut(s) 213, 899, 980
BspHI TCATGA 1 cut(s) 306
BspLI GGNNCC 6 cut(s) 34, 59, 233, 375, 895, 1107
BspPI GGATC 4 cut(s) 52, 65, 888, 901
BspT107I GGYRCC 2 cut(s) 32, 373
BspTI CTTAAG 1 cut(s) 1082
BspTNI GGTCTC 1 cut(s) 473
BsrBI CCGCTC 1 cut(s) 1431
BsrDI GCAATG 2 cut(s) 1065, 1477
BsrFI RCCGGY 2 cut(s) 29, 1323
BsrI ACTGG 2 cut(s) 241, 1039
BssAI RCCGGY 2 cut(s) 29, 1323
BssECI CCNNGG 8 cut(s) 7, 317, 469, 515, 549, 694, 897, 1496
BssMI GATC 4 cut(s) 57, 757, 893, 1219
BssNAI GTATAC 1 cut(s) 1232
BssNI GRCGYC 4 cut(s) 33, 73, 374, 653
BssT1I CCWWGG 2 cut(s) 7, 1496
Bst1107I GTATAC 1 cut(s) 1232
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 2 cut(s) 298, 1448
Bst6I CTCTTC 2 cut(s) 471, 1153
BstACI GRCGYC 4 cut(s) 33, 73, 374, 653
BstAFI CTTAAG 1 cut(s) 1082
BstAPI GCANNNNNTGC 2 cut(s) 1285, 1465
BstC8I GCNNGC 5 cut(s) 31, 936, 1130, 1212, 1461
BstDEI CTNAG 2 cut(s) 765, 816
BstDSI CCRYGG 1 cut(s) 897
BstF5I GGATG 8 cut(s) 162, 507, 732, 988, 1024, 1186, 1317, 1395
BstFNI CGCG 3 cut(s) 213, 899, 980
BstH2I RGCGCY 5 cut(s) 36, 377, 572, 741, 1082
BstHHI GCGC 9 cut(s) 35, 376, 447, 571, 740, 980, 1081, 1395, 1470
BstKTI GATC 4 cut(s) 60, 760, 896, 1222
BstMAI GTCTC 2 cut(s) 473, 1047
BstMBI GATC 4 cut(s) 57, 757, 893, 1219
BstMCI CGRYCG 1 cut(s) 521
BstMWI GCNNNNNNNGC 9 cut(s) 26, 32, 39, 219, 1076, 1114, 1285, 1465, 1474
BstNI CCWGG 1 cut(s) 319
BstNSI RCATGY 2 cut(s) 1238, 1298
BstPAI GACNNNNGTC 1 cut(s) 485
BstSCI CCNGG 7 cut(s) 15, 78, 311, 317, 489, 515, 693
BstSFI CTRYAG 1 cut(s) 801
BstUI CGCG 3 cut(s) 213, 899, 980
BstV1I GCAGC 5 cut(s) 648, 747, 1122, 1144, 1489
BstV2I GAAGAC 2 cut(s) 277, 1037
BstX2I RGATCY 2 cut(s) 57, 893
BstYI RGATCY 2 cut(s) 57, 893
BstZ17I GTATAC 1 cut(s) 1232
BstZI CGGCCG 1 cut(s) 518
BsuI GTATCC 1 cut(s) 1530
BtgI CCRYGG 1 cut(s) 897
BtgZI GCGATG 2 cut(s) 512, 683
BtsCI GGATG 8 cut(s) 162, 507, 732, 988, 1024, 1186, 1317, 1395
BtsIMutI CAGTG 1 cut(s) 1453
Cac8I GCNNGC 5 cut(s) 31, 936, 1130, 1212, 1461
CaiI CAGNNNCTG 1 cut(s) 1465
CciI TCATGA 1 cut(s) 306
CfoI GCGC 9 cut(s) 35, 376, 447, 571, 740, 980, 1081, 1395, 1470
Cfr10I RCCGGY 2 cut(s) 29, 1323
Cfr13I GGNCC 9 cut(s) 4, 14, 19, 205, 315, 321, 394, 1106, 1493
Cfr42I CCGCGG 1 cut(s) 900
CseI GACGC 3 cut(s) 81, 247, 661
Csp6I GTAC 4 cut(s) 109, 244, 813, 943
CspAI ACCGGT 1 cut(s) 1323
CviAII CATG 9 cut(s) 44, 307, 391, 559, 596, 956, 1235, 1295, 1525
CviQI GTAC 4 cut(s) 109, 244, 813, 943
DdeI CTNAG 2 cut(s) 765, 816
DinI GGCGCC 2 cut(s) 34, 375
DpnI GATC 4 cut(s) 59, 759, 895, 1221
DpnII GATC 4 cut(s) 57, 757, 893, 1219
DrdI GACNNNNNNGTC 1 cut(s) 1039
DriI GACNNNNNGTC 1 cut(s) 427
DseDI GACNNNNNNGTC 1 cut(s) 1039
EaeI YGGCCR 2 cut(s) 518, 1062
EagI CGGCCG 1 cut(s) 518
Eam1104I CTCTTC 2 cut(s) 471, 1153
Eam1105I GACNNNNNGTC 1 cut(s) 427
EarI CTCTTC 2 cut(s) 471, 1153
EciI GGCGGA 3 cut(s) 209, 262, 371
Ecl136II GAGCTC 1 cut(s) 1347
EclXI CGGCCG 1 cut(s) 518
Eco130I CCWWGG 2 cut(s) 7, 1496
Eco147I AGGCCT 1 cut(s) 194
Eco24I GRGCYC 1 cut(s) 1349
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 4 cut(s) 4, 205, 394, 1493
Eco47III AGCGCT 2 cut(s) 570, 739
Eco52I CGGCCG 1 cut(s) 518
Eco53kI GAGCTC 1 cut(s) 1347
EcoICRI GAGCTC 1 cut(s) 1347
EcoO109I RGGNCCY 1 cut(s) 1106
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 2 cut(s) 7, 1496
EcoT38I GRGCYC 1 cut(s) 1349
EgeI GGCGCC 2 cut(s) 34, 375
EheI GGCGCC 2 cut(s) 34, 375
ErhI CCWWGG 2 cut(s) 7, 1496
FaeI CATG 9 cut(s) 47, 310, 394, 562, 599, 959, 1238, 1298, 1528
FalI AAGNNNNNCTT 4 cut(s) 362, 394, 1199, 1231
FaqI GGGAC 1 cut(s) 473
FatI CATG 9 cut(s) 43, 306, 390, 558, 595, 955, 1234, 1294, 1524
FauI CCCGC 2 cut(s) 410, 927
FbaI TGATCA 1 cut(s) 1219
FblI GTMKAC 1 cut(s) 1231
FokI GGATG 8 cut(s) 149, 494, 739, 995, 1031, 1193, 1324, 1402
FriOI GRGCYC 1 cut(s) 1349
FspBI CTAG 1 cut(s) 332
FspI TGCGCA 2 cut(s) 1394, 1469
GlaI GCGC 9 cut(s) 34, 375, 446, 570, 739, 979, 1080, 1394, 1469
GsuI CTGGAG 4 cut(s) 74, 224, 762, 1286
HaeII RGCGCY 5 cut(s) 36, 377, 572, 741, 1082
HgaI GACGC 3 cut(s) 81, 247, 661
HhaI GCGC 9 cut(s) 35, 376, 447, 571, 740, 980, 1081, 1395, 1470
Hin1I GRCGYC 4 cut(s) 33, 73, 374, 653
Hin1II CATG 9 cut(s) 47, 310, 394, 562, 599, 959, 1238, 1298, 1528
Hin6I GCGC 9 cut(s) 33, 374, 445, 569, 738, 978, 1079, 1393, 1468
HinP1I GCGC 9 cut(s) 33, 374, 445, 569, 738, 978, 1079, 1393, 1468
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 8 cut(s) 51, 100, 425, 451, 619, 781, 967, 1315
Hpy166II GTNNAC 5 cut(s) 294, 301, 337, 679, 1232
Hpy188I TCNGA 6 cut(s) 50, 473, 651, 708, 1122, 1311
Hpy188III TCNNGA 4 cut(s) 55, 91, 307, 1010
Hpy8I GTNNAC 5 cut(s) 294, 301, 337, 679, 1232
Hpy99I CGWCG 7 cut(s) 75, 425, 433, 526, 655, 661, 1345
HpyAV CCTTC 3 cut(s) 388, 601, 1239
HpyCH4III ACNGT 2 cut(s) 298, 1448
HpyCH4IV ACGT 3 cut(s) 111, 182, 486
HpyCH4V TGCA 8 cut(s) 502, 587, 636, 735, 778, 1279, 1451, 1477
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 32, 39, 219, 1076, 1114, 1285, 1465, 1474
HpyF3I CTNAG 2 cut(s) 765, 816
HpySE526I ACGT 3 cut(s) 111, 182, 486
Hsp92I GRCGYC 4 cut(s) 33, 73, 374, 653
Hsp92II CATG 9 cut(s) 47, 310, 394, 562, 599, 959, 1238, 1298, 1528
HspAI GCGC 9 cut(s) 33, 374, 445, 569, 738, 978, 1079, 1393, 1468
KasI GGCGCC 2 cut(s) 32, 373
Kpn2I TCCGGA 1 cut(s) 54
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 1219
KspI CCGCGG 1 cut(s) 900
Kzo9I GATC 4 cut(s) 57, 757, 893, 1219
LmnI GCTCC 8 cut(s) 237, 275, 467, 566, 696, 1012, 1352, 1428
Lsp1109I GCAGC 5 cut(s) 648, 747, 1122, 1144, 1489
LweI GCATC 2 cut(s) 489, 1380
MaeI CTAG 1 cut(s) 332
MaeII ACGT 3 cut(s) 111, 182, 486
MaeIII GTNAC 2 cut(s) 178, 1442
MalI GATC 4 cut(s) 59, 759, 895, 1221
MbiI CCGCTC 1 cut(s) 1431
MboI GATC 4 cut(s) 57, 757, 893, 1219
MboII GAAGA 6 cut(s) 277, 488, 1042, 1135, 1140, 1282
MflI RGATCY 2 cut(s) 57, 893
MhlI GDGCHC 1 cut(s) 1349
MlsI TGGCCA 1 cut(s) 1064
MluCI AATT 4 cut(s) 631, 715, 832, 871
MluNI TGGCCA 1 cut(s) 1064
Mly113I GGCGCC 2 cut(s) 33, 374
MlyI GAGTC 4 cut(s) 60, 419, 961, 1309
MmeI TCCRAC 4 cut(s) 28, 414, 481, 674
Mox20I TGGCCA 1 cut(s) 1064
MreI CGCCGGCG 1 cut(s) 29
MroI TCCGGA 1 cut(s) 54
MroNI GCCGGC 1 cut(s) 29
MscI TGGCCA 1 cut(s) 1064
MseI TTAA 6 cut(s) 149, 170, 185, 645, 1083, 1161
MslI CAYNNNNRTG 1 cut(s) 1239
Msp20I TGGCCA 1 cut(s) 1064
MspA1I CMGCKG 2 cut(s) 899, 1135
MspCI CTTAAG 1 cut(s) 1082
MspR9I CCNGG 7 cut(s) 17, 80, 313, 319, 491, 517, 695
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 3 cut(s) 213, 899, 980
MwoI GCNNNNNNNGC 9 cut(s) 26, 32, 39, 219, 1076, 1114, 1285, 1465, 1474
NaeI GCCGGC 1 cut(s) 31
NarI GGCGCC 2 cut(s) 33, 374
NciI CCSGG 6 cut(s) 17, 80, 313, 491, 517, 695
NdeII GATC 4 cut(s) 57, 757, 893, 1219
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 9 cut(s) 47, 310, 394, 562, 599, 959, 1238, 1298, 1528
NlaIV GGNNCC 6 cut(s) 34, 59, 233, 375, 895, 1107
NmeAIII GCCGAG 2 cut(s) 99, 574
NmuCI GTSAC 1 cut(s) 178
NsbI TGCGCA 2 cut(s) 1394, 1469
NspI RCATGY 2 cut(s) 1238, 1298
PagI TCATGA 1 cut(s) 306
PceI AGGCCT 1 cut(s) 194
PciI ACATGT 1 cut(s) 1294
PcsI WCGNNNNNNNCGW 3 cut(s) 294, 420, 665
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 4 cut(s) 100, 451, 619, 781
PflFI GACNNNGTC 1 cut(s) 655
PfoI TCCNGGA 2 cut(s) 78, 489
PinAI ACCGGT 1 cut(s) 1323
PleI GAGTC 4 cut(s) 59, 419, 961, 1309
PluTI GGCGCC 2 cut(s) 36, 377
PpsI GAGTC 4 cut(s) 59, 419, 961, 1309
PscI ACATGT 1 cut(s) 1294
PshAI GACNNNNGTC 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 1349
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 6 cut(s) 34, 59, 233, 375, 895, 1107
PspPI GGNCC 9 cut(s) 4, 14, 19, 205, 315, 321, 394, 1106, 1493
PstNI CAGNNNCTG 1 cut(s) 1465
PsuI RGATCY 2 cut(s) 57, 893
PsyI GACNNNGTC 1 cut(s) 655
PvuII CAGCTG 1 cut(s) 1135
RsaI GTAC 4 cut(s) 110, 245, 814, 944
RsaNI GTAC 4 cut(s) 109, 244, 813, 943
RseI CAYNNNNRTG 1 cut(s) 1239
SacI GAGCTC 1 cut(s) 1349
SacII CCGCGG 1 cut(s) 900
SaqAI TTAA 6 cut(s) 149, 170, 185, 645, 1083, 1161
Sau3AI GATC 4 cut(s) 57, 757, 893, 1219
Sau96I GGNCC 9 cut(s) 4, 14, 19, 205, 315, 321, 394, 1106, 1493
ScaI AGTACT 1 cut(s) 814
SchI GAGTC 4 cut(s) 60, 419, 961, 1309
ScrFI CCNGG 7 cut(s) 17, 80, 313, 319, 491, 517, 695
SduI GDGCHC 1 cut(s) 1349
SfaNI GCATC 2 cut(s) 489, 1380
SfcI CTRYAG 1 cut(s) 801
SfoI GGCGCC 2 cut(s) 34, 375
Sfr303I CCGCGG 1 cut(s) 900
SgrAI CRCCGGYG 1 cut(s) 29
SgrBI CCGCGG 1 cut(s) 900
SinI GGWCC 4 cut(s) 4, 205, 394, 1493
SmiMI CAYNNNNRTG 1 cut(s) 1239
SmlI CTYRAG 3 cut(s) 104, 368, 1082
SmoI CTYRAG 3 cut(s) 104, 368, 1082
Sse9I AATT 4 cut(s) 631, 715, 832, 871
SseBI AGGCCT 1 cut(s) 194
SspDI GGCGCC 2 cut(s) 32, 373
SspI AATATT 1 cut(s) 703
SspMI CTAG 1 cut(s) 332
SstI GAGCTC 1 cut(s) 1349
StuI AGGCCT 1 cut(s) 194
StyD4I CCNGG 7 cut(s) 15, 78, 311, 317, 489, 515, 693
StyI CCWWGG 2 cut(s) 7, 1496
TaaI ACNGT 2 cut(s) 298, 1448
TaiI ACGT 3 cut(s) 114, 185, 489
TaqI TCGA 7 cut(s) 189, 420, 524, 883, 911, 1049, 1343
TasI AATT 4 cut(s) 631, 715, 832, 871
TatI WGTACW 3 cut(s) 243, 812, 942
TauI GCSGC 5 cut(s) 15, 29, 387, 533, 536
TfiI GAWTC 4 cut(s) 100, 451, 619, 781
Tru1I TTAA 6 cut(s) 149, 170, 185, 645, 1083, 1161
Tru9I TTAA 6 cut(s) 149, 170, 185, 645, 1083, 1161
TscAI CASTG 1 cut(s) 1453
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 5 cut(s) 636, 735, 1132, 1135, 1477
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 5 cut(s) 151, 379, 773, 944, 1541
TspGWI ACGGA 1 cut(s) 1388
TspRI CASTG 1 cut(s) 1453
Tth111I GACNNNGTC 1 cut(s) 655
Vha464I CTTAAG 1 cut(s) 1082
VpaK11BI GGWCC 4 cut(s) 4, 205, 394, 1493
XceI RCATGY 2 cut(s) 1238, 1298
XcmI CCANNNNNNNNNTGG 1 cut(s) 331
XmiI GTMKAC 1 cut(s) 1231
XspI CTAG 1 cut(s) 332
ZrmI AGTACT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.