MD08G1031000.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010704
Physical Location & Seq
Reverse (-)
2215461 .. 2217848
2388 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1031000.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGGACCCAGGCGGAGCAACCAATGTCGCCACCGCGCGCGGCGGAGGCGGTGAGGTCGGGGTCCGGATTCACCAAACCCGACGGCTCCCCGATTTCCTCCAGAGCGTTAATCTCAAGTACGTGAAATTAGGGTACCATTACTTGATCTCCAATCTCTTAACCCTCTGCTTTATCCCGTTGATTATCGTAACCCTAATCGAAGCCTCCCAAATGGACATTTACGACATTCAACAACTCTGGCTGCACCTCCAGTACAATCTCGTCTCCGTCATCATCTGCTCCGCCGTCCTCGTCTTCGGGTTGACCGTCTACATCATGACCCGACCCAGACCCGTTTACTTGGTCGATTACGCCTGCTACCGCCCGCCCGATCACCTCAAGGCACCGTACCAGCGCTTCATGGAGCACTCCCGCCTCACGCAGGACTTCGACGACTCGTCGCTCGAGTTCCAGCGTAAGATTCTCGAGCGCTCCGGCCTCGGCGAGGAGACTTATGTCCCGGAGGCGATGCATTACATCCCTCCCCGGCCGTCGATGGCCGCCGCCAGGGAGGAGGCGGAGCAGGTGATGTACGGTGCTCTGGATAATCTGTTTGCCAATACCCATGTGAAGCCAAAGGACATTGGGATTCTCGTTGTGAATTGCAGCTTGTTTAATCCGACGCCGTCGCTTTCCGCGATGATTGTTAATAAGTACAAATTGAGGGGTAATATTAGGAGTTTCAATTTGGGGGGAATGGGTTGTAGTGCTGGGGTTATAGCTGTTGATCTTGCCAAGGACTTGTTGCAAATCCACCGGAATACTTATGCTGTTGTTGTTAGTACTGAGAACATTACTCAGAATTGGTATTTTGGGAATAAGAAGTCTATGTTGATACCCAATTGCTTGTTTCGTGTTGGGGGTTCCGCGGTTTTGCTTTCCAATAAGTCCGTTGATAGGAGGCGGGCTAAGTACAAGCTTGTTCATGTTGTGAGGACCCATCGTGGCGCGGATGATAAAGCTTTTCGTTGTGTTTATCAGGAGCAGGATGATAAGGGGAAGACTGGTGTGTCTTTGTCGAAAGATTTGATGGCAATTGCCGGTGGGGCGCTTAAGACCAACATTACCACTTTGGGACCTATTGTGCTTCCTATAAGCGAGCAGCTTCTTTTCTTTTCATCACTTGTGGTTAAGAAGCTGTTCAAGTCCAACGTCAAGCCATATATCCCGGATTTCAAGCTGGCATTTGATCATTTTTGCATACACGCCGGAGGAAGGGCTGTGATTGATGAGCTGGAGAAGAACCTGCAGTTGCTGCCTACACATGTTGAGGCCTCTCGAATGACTCTACACCGGTTTGGGAATACTTCGTCGAGCTCCATTTGGTATGAATTGGCGTATATGGAGGCCAAGGGGAGGATCCGCAGGGGCAATCGTATCTGGCAGATTGCCTTTGGAAGTGGTTTTAAATGTAACAGTGCGGTCTGGGAGGCACTTCGGAATGTGAAGCCATCTTGTAATGGTCCTTGGGAAGACTGCATTGACAAGTATCCTGTGAAAGTAGTATACTCTTGCATATGCTATATTGATATGGTTCACAAAGTTTGCTCCAACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

533

Amino Acids

59.9

Weight (kDa)

9.14

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 104 - 393 1.5e-146 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 410 - 490 9.3e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 412 - 481 1.3e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 553
AasI GACNNNNNNGTC 1 cut(s) 1048
Acc36I ACCTGC 2 cut(s) 553, 1293
Acc65I GGTACC 1 cut(s) 132
AccB1I GGYRCC 2 cut(s) 132, 382
AccI GTMKAC 2 cut(s) 309, 1545
AccII CGCG 6 cut(s) 35, 37, 39, 677, 908, 989
AccIII TCCGGA 1 cut(s) 63
AclWI GGATC 2 cut(s) 1393, 1406
AcoI YGGCCR 2 cut(s) 527, 537
AcyI GRCGYC 1 cut(s) 662
AfaI GTAC 8 cut(s) 119, 134, 254, 389, 572, 695, 823, 953
AfeI AGCGCT 2 cut(s) 395, 470
AfiI CCNNNNNNNGG 6 cut(s) 421, 484, 546, 936, 1254, 1395
AflII CTTAAG 1 cut(s) 1091
AflIII ACRYGT 1 cut(s) 1303
AgeI ACCGGT 1 cut(s) 1332
AgsI TTSAA 4 cut(s) 230, 724, 1183, 1216
AhdI GACNNNNNGTC 1 cut(s) 436
AjnI CCWGG 2 cut(s) 7, 545
AluBI AGCT 9 cut(s) 648, 761, 958, 1001, 1144, 1177, 1219, 1273, 1356
AluI AGCT 9 cut(s) 648, 761, 958, 1001, 1144, 1177, 1219, 1273, 1356
Alw21I GWGCWC 3 cut(s) 408, 580, 1358
Alw26I GTCTC 2 cut(s) 268, 482
AlwI GGATC 2 cut(s) 1393, 1406
AlwNI CAGNNNCTG 1 cut(s) 1294
Ama87I CYCGRG 2 cut(s) 443, 464
Aor13HI TCCGGA 1 cut(s) 63
Aor51HI AGCGCT 2 cut(s) 395, 470
AoxI GGCC 5 cut(s) 475, 527, 537, 1311, 1386
ApeKI GCWGC 4 cut(s) 241, 645, 1141, 1294
AsiGI ACCGGT 1 cut(s) 1332
Asp700I GAANNNNTTC 1 cut(s) 1178
Asp718I GGTACC 1 cut(s) 132
AspLEI GCGC 6 cut(s) 37, 39, 396, 471, 989, 1090
AspS9I GGNCC 5 cut(s) 4, 61, 975, 1115, 1502
AsuC2I CCSGG 3 cut(s) 500, 526, 1208
AsuHPI GGTGA 4 cut(s) 62, 62, 365, 577
AvaI CYCGRG 2 cut(s) 443, 464
AvaII GGWCC 5 cut(s) 4, 61, 975, 1115, 1502
BamHI GGATCC 1 cut(s) 1398
BanI GGYRCC 2 cut(s) 132, 382
BanII GRGCYC 1 cut(s) 1358
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
BbsI GAAGAC 3 cut(s) 286, 1046, 1518
Bbv12I GWGCWC 3 cut(s) 408, 580, 1358
BbvI GCAGC 4 cut(s) 228, 657, 1153, 1281
BccI CCATC 4 cut(s) 529, 987, 1063, 1498
BceAI ACGGC 4 cut(s) 98, 269, 514, 649
BciT130I CCWGG 2 cut(s) 9, 547
BciVI GTATCC 1 cut(s) 1539
BclI TGATCA 1 cut(s) 1228
BcnI CCSGG 3 cut(s) 500, 526, 1208
BcoDI GTCTC 2 cut(s) 268, 482
BfaI CTAG 1 cut(s) 1597
BfmI CTRYAG 1 cut(s) 1286
BfoI RGCGCY 3 cut(s) 397, 472, 1091
BfrI CTTAAG 1 cut(s) 1091
BfuAI ACCTGC 2 cut(s) 553, 1293
BfuI GTATCC 1 cut(s) 1539
BglI GCCNNNNNGGC 1 cut(s) 1085
BisI GCNGC 7 cut(s) 40, 242, 540, 543, 646, 1142, 1295
BlsI GCNGC 7 cut(s) 41, 243, 541, 544, 647, 1143, 1296
BmcAI AGTACT 1 cut(s) 823
Bme1390I CCNGG 5 cut(s) 9, 500, 526, 547, 1208
Bme18I GGWCC 5 cut(s) 4, 61, 975, 1115, 1502
BmeRI GACNNNNNGTC 1 cut(s) 436
BmeT110I CYCGRG 2 cut(s) 443, 464
BmgT120I GGNCC 5 cut(s) 4, 61, 975, 1115, 1502
BmiI GGNNCC 9 cut(s) 6, 62, 86, 134, 384, 904, 977, 1116, 1400
BmrFI CCNGG 5 cut(s) 9, 500, 526, 547, 1208
BmsI GCATC 1 cut(s) 498
BoxI GACNNNNGTC 1 cut(s) 494
BpiI GAAGAC 3 cut(s) 286, 1046, 1518
BpmI CTGGAG 3 cut(s) 83, 233, 1295
BpuEI CTTGAG 2 cut(s) 98, 362
BpuMI CCSGG 3 cut(s) 500, 526, 1208
BsaAI YACGTR 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 662
BsaJI CCNNGG 8 cut(s) 7, 478, 524, 546, 774, 906, 1389, 1505
BsaWI WCCGGW 3 cut(s) 63, 795, 1332
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 6 cut(s) 421, 484, 546, 936, 1254, 1395
Bse118I RCCGGY 2 cut(s) 1079, 1332
Bse1I ACTGG 2 cut(s) 250, 1048
BseAI TCCGGA 1 cut(s) 63
BseBI CCWGG 2 cut(s) 9, 547
BseDI CCNNGG 8 cut(s) 7, 478, 524, 546, 774, 906, 1389, 1505
BseGI GGATG 3 cut(s) 516, 997, 1033
BseLI CCNNNNNNNGG 6 cut(s) 421, 484, 546, 936, 1254, 1395
BseMII CTCAG 2 cut(s) 816, 851
BseNI ACTGG 2 cut(s) 250, 1048
BsePI GCGCGC 1 cut(s) 35
BseRI GAGGAG 2 cut(s) 500, 566
BseX3I CGGCCG 1 cut(s) 527
BseXI GCAGC 4 cut(s) 228, 657, 1153, 1281
BseYI CCCAGC 1 cut(s) 749
BsgI GTGCAG 1 cut(s) 227
Bsh1236I CGCG 6 cut(s) 35, 37, 39, 677, 908, 989
Bsh1285I CGRYCG 1 cut(s) 530
BshFI GGCC 5 cut(s) 477, 529, 539, 1313, 1388
BshNI GGYRCC 2 cut(s) 132, 382
BshTI ACCGGT 1 cut(s) 1332
BsiEI CGRYCG 1 cut(s) 530
BsiHKAI GWGCWC 3 cut(s) 408, 580, 1358
BsiHKCI CYCGRG 2 cut(s) 443, 464
BsiSI CCGG 9 cut(s) 64, 474, 500, 526, 796, 1080, 1208, 1248, 1333
BslFI GGGAC 2 cut(s) 482, 1128
BslI CCNNNNNNNGG 6 cut(s) 421, 484, 546, 936, 1254, 1395
BsmAI GTCTC 2 cut(s) 268, 482
BsmBI CGTCTC 1 cut(s) 268
BsmFI GGGAC 2 cut(s) 482, 1128
BsnI GGCC 5 cut(s) 477, 529, 539, 1313, 1388
BsoBI CYCGRG 2 cut(s) 443, 464
Bsp1286I GDGCHC 3 cut(s) 408, 580, 1358
Bsp13I TCCGGA 1 cut(s) 63
Bsp143I GATC 5 cut(s) 144, 370, 766, 1228, 1398
BspANI GGCC 5 cut(s) 477, 529, 539, 1313, 1388
BspCNI CTCAG 2 cut(s) 817, 850
BspEI TCCGGA 1 cut(s) 63
BspFNI CGCG 6 cut(s) 35, 37, 39, 677, 908, 989
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 9 cut(s) 6, 62, 86, 134, 384, 904, 977, 1116, 1400
BspMAI CTGCAG 1 cut(s) 1290
BspMI ACCTGC 2 cut(s) 553, 1293
BspPI GGATC 2 cut(s) 1393, 1406
BspT107I GGYRCC 2 cut(s) 132, 382
BspTI CTTAAG 1 cut(s) 1091
BsrFI RCCGGY 2 cut(s) 1079, 1332
BsrI ACTGG 2 cut(s) 250, 1048
BssAI RCCGGY 2 cut(s) 1079, 1332
BssECI CCNNGG 8 cut(s) 7, 478, 524, 546, 774, 906, 1389, 1505
BssHII GCGCGC 1 cut(s) 35
BssMI GATC 5 cut(s) 144, 370, 766, 1228, 1398
BssNAI GTATAC 1 cut(s) 1546
BssNI GRCGYC 1 cut(s) 662
BssT1I CCWWGG 3 cut(s) 774, 1389, 1505
Bst1107I GTATAC 1 cut(s) 1546
Bst2UI CCWGG 2 cut(s) 9, 547
Bst4CI ACNGT 4 cut(s) 307, 387, 575, 1457
BstACI GRCGYC 1 cut(s) 662
BstAFI CTTAAG 1 cut(s) 1091
BstAPI GCANNNNNTGC 1 cut(s) 1294
BstBAI YACGTR 1 cut(s) 121
BstC8I GCNNGC 6 cut(s) 37, 355, 365, 945, 1139, 1221
BstDEI CTNAG 3 cut(s) 825, 837, 948
BstDSI CCRYGG 1 cut(s) 906
BstENI CCTNNNNNAGG 2 cut(s) 419, 482
BstF5I GGATG 3 cut(s) 516, 997, 1033
BstFNI CGCG 6 cut(s) 35, 37, 39, 677, 908, 989
BstH2I RGCGCY 3 cut(s) 397, 472, 1091
BstHHI GCGC 6 cut(s) 37, 39, 396, 471, 989, 1090
BstKTI GATC 5 cut(s) 147, 373, 769, 1231, 1401
BstMAI GTCTC 2 cut(s) 268, 482
BstMBI GATC 5 cut(s) 144, 370, 766, 1228, 1398
BstMCI CGRYCG 1 cut(s) 530
BstMWI GCNNNNNNNGC 3 cut(s) 45, 1085, 1294
BstNI CCWGG 2 cut(s) 9, 547
BstNSI RCATGY 1 cut(s) 1307
BstPAI GACNNNNGTC 1 cut(s) 494
BstSCI CCNGG 5 cut(s) 7, 498, 524, 545, 1206
BstSFI CTRYAG 1 cut(s) 1286
BstUI CGCG 6 cut(s) 35, 37, 39, 677, 908, 989
BstV1I GCAGC 4 cut(s) 228, 657, 1153, 1281
BstV2I GAAGAC 3 cut(s) 286, 1046, 1518
BstX2I RGATCY 1 cut(s) 1398
BstYI RGATCY 1 cut(s) 1398
BstZ17I GTATAC 1 cut(s) 1546
BstZI CGGCCG 1 cut(s) 527
BsuI GTATCC 1 cut(s) 1539
BsuRI GGCC 5 cut(s) 477, 529, 539, 1313, 1388
BtgI CCRYGG 1 cut(s) 906
BtgZI GCGATG 2 cut(s) 521, 692
BtsCI GGATG 3 cut(s) 516, 997, 1033
BtsIMutI CAGTG 1 cut(s) 1462
BveI ACCTGC 2 cut(s) 553, 1293
Cac8I GCNNGC 6 cut(s) 37, 355, 365, 945, 1139, 1221
CaiI CAGNNNCTG 1 cut(s) 1294
CciI TCATGA 1 cut(s) 315
CfoI GCGC 6 cut(s) 37, 39, 396, 471, 989, 1090
Cfr10I RCCGGY 2 cut(s) 1079, 1332
Cfr13I GGNCC 5 cut(s) 4, 61, 975, 1115, 1502
Cfr42I CCGCGG 1 cut(s) 909
CseI GACGC 1 cut(s) 670
Csp6I GTAC 8 cut(s) 118, 133, 253, 388, 571, 694, 822, 952
CspAI ACCGGT 1 cut(s) 1332
CviAII CATG 5 cut(s) 316, 400, 605, 965, 1304
CviQI GTAC 8 cut(s) 118, 133, 253, 388, 571, 694, 822, 952
DdeI CTNAG 3 cut(s) 825, 837, 948
DpnI GATC 5 cut(s) 146, 372, 768, 1230, 1400
DpnII GATC 5 cut(s) 144, 370, 766, 1228, 1398
DraI TTTAAA 1 cut(s) 1447
DrdI GACNNNNNNGTC 1 cut(s) 1048
DriI GACNNNNNGTC 1 cut(s) 436
DseDI GACNNNNNNGTC 1 cut(s) 1048
EaeI YGGCCR 2 cut(s) 527, 537
EagI CGGCCG 1 cut(s) 527
Eam1105I GACNNNNNGTC 1 cut(s) 436
EciI GGCGGA 4 cut(s) 27, 57, 271, 572
Ecl136II GAGCTC 1 cut(s) 1356
EclXI CGGCCG 1 cut(s) 527
Eco130I CCWWGG 3 cut(s) 774, 1389, 1505
Eco147I AGGCCT 1 cut(s) 1313
Eco24I GRGCYC 1 cut(s) 1358
Eco47I GGWCC 5 cut(s) 4, 61, 975, 1115, 1502
Eco47III AGCGCT 2 cut(s) 395, 470
Eco52I CGGCCG 1 cut(s) 527
Eco53kI GAGCTC 1 cut(s) 1356
Eco88I CYCGRG 2 cut(s) 443, 464
EcoICRI GAGCTC 1 cut(s) 1356
EcoNI CCTNNNNNAGG 2 cut(s) 419, 482
EcoO109I RGGNCCY 2 cut(s) 975, 1115
EcoRII CCWGG 2 cut(s) 7, 545
EcoT14I CCWWGG 3 cut(s) 774, 1389, 1505
EcoT22I ATGCAT 1 cut(s) 513
EcoT38I GRGCYC 1 cut(s) 1358
ErhI CCWWGG 3 cut(s) 774, 1389, 1505
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 5 cut(s) 319, 403, 608, 968, 1307
FaqI GGGAC 2 cut(s) 482, 1128
FatI CATG 5 cut(s) 315, 399, 604, 964, 1303
FauI CCCGC 3 cut(s) 372, 419, 936
FauNDI CATATG 1 cut(s) 1556
FbaI TGATCA 1 cut(s) 1228
FblI GTMKAC 2 cut(s) 309, 1545
Fnu4HI GCNGC 7 cut(s) 40, 242, 540, 543, 646, 1142, 1295
FokI GGATG 3 cut(s) 503, 1004, 1040
FriOI GRGCYC 1 cut(s) 1358
Fsp4HI GCNGC 7 cut(s) 40, 242, 540, 543, 646, 1142, 1295
FspBI CTAG 1 cut(s) 1597
GlaI GCGC 6 cut(s) 36, 38, 395, 470, 988, 1089
GluI GCNGC 7 cut(s) 40, 242, 540, 543, 646, 1142, 1295
GsaI CCCAGC 1 cut(s) 753
GsuI CTGGAG 3 cut(s) 83, 233, 1295
HaeII RGCGCY 3 cut(s) 397, 472, 1091
HaeIII GGCC 5 cut(s) 477, 529, 539, 1313, 1388
HapII CCGG 9 cut(s) 64, 474, 500, 526, 796, 1080, 1208, 1248, 1333
HgaI GACGC 1 cut(s) 670
HhaI GCGC 6 cut(s) 37, 39, 396, 471, 989, 1090
Hin1I GRCGYC 1 cut(s) 662
Hin1II CATG 5 cut(s) 319, 403, 608, 968, 1307
Hin6I GCGC 6 cut(s) 35, 37, 394, 469, 987, 1088
HinP1I GCGC 6 cut(s) 35, 37, 394, 469, 987, 1088
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HindIII AAGCTT 2 cut(s) 956, 999
HinfI GANTC 5 cut(s) 67, 434, 460, 628, 1324
HpaII CCGG 9 cut(s) 64, 474, 500, 526, 796, 1080, 1208, 1248, 1333
HphI GGTGA 4 cut(s) 62, 62, 365, 577
Hpy166II GTNNAC 5 cut(s) 303, 310, 337, 1546, 1576
Hpy188I TCNGA 3 cut(s) 660, 840, 1479
Hpy188III TCNNGA 7 cut(s) 64, 100, 316, 464, 581, 1019, 1317
Hpy8I GTNNAC 5 cut(s) 303, 310, 337, 1546, 1576
Hpy99I CGWCG 7 cut(s) 84, 434, 442, 535, 664, 670, 1354
HpyAV CCTTC 1 cut(s) 1248
HpyCH4III ACNGT 4 cut(s) 307, 387, 575, 1457
HpyCH4IV ACGT 2 cut(s) 120, 1191
HpyCH4V TGCA 8 cut(s) 244, 511, 645, 787, 1239, 1288, 1518, 1554
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 1085, 1294
HpyF3I CTNAG 3 cut(s) 825, 837, 948
HpySE526I ACGT 2 cut(s) 120, 1191
Hsp92I GRCGYC 1 cut(s) 662
Hsp92II CATG 5 cut(s) 319, 403, 608, 968, 1307
HspAI GCGC 6 cut(s) 35, 37, 394, 469, 987, 1088
Kpn2I TCCGGA 1 cut(s) 63
KpnI GGTACC 1 cut(s) 136
Ksp22I TGATCA 1 cut(s) 1228
KspI CCGCGG 1 cut(s) 909
Kzo9I GATC 5 cut(s) 144, 370, 766, 1228, 1398
LmnI GCTCC 9 cut(s) 14, 90, 284, 403, 476, 559, 1021, 1361, 1592
Lsp1109I GCAGC 4 cut(s) 228, 657, 1153, 1281
LweI GCATC 1 cut(s) 498
MaeI CTAG 1 cut(s) 1597
MaeII ACGT 2 cut(s) 120, 1191
MaeIII GTNAC 2 cut(s) 187, 1451
MalI GATC 5 cut(s) 146, 372, 768, 1230, 1400
MauBI CGCGCGCG 1 cut(s) 35
MboI GATC 5 cut(s) 144, 370, 766, 1228, 1398
MboII GAAGA 4 cut(s) 286, 1051, 1291, 1523
MfeI CAATTG 2 cut(s) 880, 1074
MflI RGATCY 1 cut(s) 1398
MhlI GDGCHC 3 cut(s) 408, 580, 1358
MluCI AATT 8 cut(s) 125, 640, 698, 724, 841, 880, 1074, 1370
MlyI GAGTC 2 cut(s) 428, 1318
MmeI TCCRAC 2 cut(s) 683, 1212
Mph1103I ATGCAT 1 cut(s) 513
MroI TCCGGA 1 cut(s) 63
MroXI GAANNNNTTC 1 cut(s) 1178
MseI TTAA 7 cut(s) 108, 158, 654, 687, 1092, 1170, 1446
MspA1I CMGCKG 1 cut(s) 908
MspCI CTTAAG 1 cut(s) 1091
MspI CCGG 9 cut(s) 64, 474, 500, 526, 796, 1080, 1208, 1248, 1333
MspR9I CCNGG 5 cut(s) 9, 500, 526, 547, 1208
MunI CAATTG 2 cut(s) 880, 1074
MvaI CCWGG 2 cut(s) 9, 547
MvnI CGCG 6 cut(s) 35, 37, 39, 677, 908, 989
MwoI GCNNNNNNNGC 3 cut(s) 45, 1085, 1294
NciI CCSGG 3 cut(s) 500, 526, 1208
NdeI CATATG 1 cut(s) 1556
NdeII GATC 5 cut(s) 144, 370, 766, 1228, 1398
NlaIII CATG 5 cut(s) 319, 403, 608, 968, 1307
NlaIV GGNNCC 9 cut(s) 6, 62, 86, 134, 384, 904, 977, 1116, 1400
NmeAIII GCCGAG 1 cut(s) 459
NsiI ATGCAT 1 cut(s) 513
NspI RCATGY 1 cut(s) 1307
PaeR7I CTCGAG 2 cut(s) 443, 464
PagI TCATGA 1 cut(s) 315
PaqCI CACCTGC 1 cut(s) 553
PauI GCGCGC 1 cut(s) 35
PceI AGGCCT 1 cut(s) 1313
PciI ACATGT 1 cut(s) 1303
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 674
PdmI GAANNNNTTC 1 cut(s) 1178
PfeI GAWTC 3 cut(s) 67, 460, 628
PflFI GACNNNGTC 1 cut(s) 664
PfoI TCCNGGA 2 cut(s) 498, 1206
PinAI ACCGGT 1 cut(s) 1332
PkrI GCNGC 7 cut(s) 41, 243, 541, 544, 647, 1143, 1296
PleI GAGTC 2 cut(s) 428, 1318
PpsI GAGTC 2 cut(s) 428, 1318
Ppu21I YACGTR 1 cut(s) 121
PpuMI RGGWCCY 2 cut(s) 975, 1115
PscI ACATGT 1 cut(s) 1303
PshAI GACNNNNGTC 1 cut(s) 494
Psp124BI GAGCTC 1 cut(s) 1358
Psp5II RGGWCCY 2 cut(s) 975, 1115
Psp6I CCWGG 2 cut(s) 7, 545
PspFI CCCAGC 1 cut(s) 749
PspGI CCWGG 2 cut(s) 7, 545
PspN4I GGNNCC 9 cut(s) 6, 62, 86, 134, 384, 904, 977, 1116, 1400
PspPI GGNCC 5 cut(s) 4, 61, 975, 1115, 1502
PspPPI RGGWCCY 2 cut(s) 975, 1115
PspXI VCTCGAGB 1 cut(s) 443
PstI CTGCAG 1 cut(s) 1290
PstNI CAGNNNCTG 1 cut(s) 1294
PsuI RGATCY 1 cut(s) 1398
PsyI GACNNNGTC 1 cut(s) 664
PteI GCGCGC 1 cut(s) 35
RsaI GTAC 8 cut(s) 119, 134, 254, 389, 572, 695, 823, 953
RsaNI GTAC 8 cut(s) 118, 133, 253, 388, 571, 694, 822, 952
SacI GAGCTC 1 cut(s) 1358
SacII CCGCGG 1 cut(s) 909
SaqAI TTAA 7 cut(s) 108, 158, 654, 687, 1092, 1170, 1446
SatI GCNGC 7 cut(s) 40, 242, 540, 543, 646, 1142, 1295
Sau3AI GATC 5 cut(s) 144, 370, 766, 1228, 1398
Sau96I GGNCC 5 cut(s) 4, 61, 975, 1115, 1502
ScaI AGTACT 1 cut(s) 823
SchI GAGTC 2 cut(s) 428, 1318
ScrFI CCNGG 5 cut(s) 9, 500, 526, 547, 1208
SduI GDGCHC 3 cut(s) 408, 580, 1358
SfaNI GCATC 1 cut(s) 498
SfcI CTRYAG 1 cut(s) 1286
Sfr274I CTCGAG 2 cut(s) 443, 464
Sfr303I CCGCGG 1 cut(s) 909
SgrBI CCGCGG 1 cut(s) 909
SinI GGWCC 5 cut(s) 4, 61, 975, 1115, 1502
SlaI CTCGAG 2 cut(s) 443, 464
SmlI CTYRAG 5 cut(s) 113, 377, 443, 464, 1091
SmoI CTYRAG 5 cut(s) 113, 377, 443, 464, 1091
Sse9I AATT 8 cut(s) 125, 640, 698, 724, 841, 880, 1074, 1370
SseBI AGGCCT 1 cut(s) 1313
SspI AATATT 1 cut(s) 712
SspMI CTAG 1 cut(s) 1597
SstI GAGCTC 1 cut(s) 1358
StuI AGGCCT 1 cut(s) 1313
StyD4I CCNGG 5 cut(s) 7, 498, 524, 545, 1206
StyI CCWWGG 3 cut(s) 774, 1389, 1505
TaaI ACNGT 4 cut(s) 307, 387, 575, 1457
TaiI ACGT 2 cut(s) 123, 1194
TaqI TCGA 9 cut(s) 198, 345, 429, 444, 465, 533, 1058, 1318, 1352
TasI AATT 8 cut(s) 125, 640, 698, 724, 841, 880, 1074, 1370
TatI WGTACW 4 cut(s) 252, 693, 821, 951
TauI GCSGC 3 cut(s) 42, 542, 545
TfiI GAWTC 3 cut(s) 67, 460, 628
Tru1I TTAA 7 cut(s) 108, 158, 654, 687, 1092, 1170, 1446
Tru9I TTAA 7 cut(s) 108, 158, 654, 687, 1092, 1170, 1446
TscAI CASTG 1 cut(s) 1462
TseI GCWGC 4 cut(s) 241, 645, 1141, 1294
TspDTI ATGAA 4 cut(s) 388, 953, 1146, 1383
TspGWI ACGGA 2 cut(s) 256, 919
TspRI CASTG 1 cut(s) 1462
Tth111I GACNNNGTC 1 cut(s) 664
Vha464I CTTAAG 1 cut(s) 1091
VpaK11BI GGWCC 5 cut(s) 4, 61, 975, 1115, 1502
XagI CCTNNNNNAGG 2 cut(s) 419, 482
XceI RCATGY 1 cut(s) 1307
XhoI CTCGAG 2 cut(s) 443, 464
XmiI GTMKAC 2 cut(s) 309, 1545
XmnI GAANNNNTTC 1 cut(s) 1178
XspI CTAG 1 cut(s) 1597
ZrmI AGTACT 1 cut(s) 823
Zsp2I ATGCAT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.