MD14G1093000.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010704
Physical Location & Seq
Reverse (-)
13857698 .. 13858645
948 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1093000.v1.1.491

Sequence Viewer

Length: 948 bp
ATGACCCGACCCAGACCCGTCTACTTGGTCGATTACGCCTGCTACCGCCCGCCCGATCACCTCAAGGCACCGTACCAACGCTTCATGGAGCACTCCCGCCTCACGCAGGACTTCGACGACTCGTCGCTCGAGTTCCAGCGTAAGATTCTCGAGCGCTCCGGCCTCGGCGAGGAGACTTATGTCCCGGAGGTGATGCATTACATCCCTCCCCGGCCGTCGATGGCCGCCGCCAGGGAGGAGGCGGAGCAGGTGATGTACGGTGCTTTGGATAATCTATTTGCCAATACCCATGTGAAGCCAAAGGATATTGGGATTCTCGTTGTGAATTGCAGCTTGTTTAATCCGACGCCGTCGCTTTCCGCCATGATTGTTAATAAGTACAAATTGAGGGGTAATATTAGGAGTTTTAACTTGGGGGGAATGGGTTGTAGTGCTGGGGTTATAGCTGTTGATCTTGCCAAAGACTTGTTGCAAATCCATCGGAACACTTATGCTGTTGTTGTTAGTACTGAGAACATTACTCAGAATTGGTATTTTGGGAATAAGAAGTCTATGTTGATACCCAATTGCTTGTTTCGTGTTGGGGGTTCCGCGGTTTTGCTTTCCAATAAGTCCGTTGATAGGAGGCGGGCTAAGTACAAGCTTGTTCATGTTGTGAGGACCCATCGTGGCGCGAATGATAAAGTTTTCCGTTGTGTTTATCAGGAGCAGGATGACAAGGGGAATACTGGTGTGTCTTTGTCGAAAGATTTGATGGCAATTGCCGGTGGGGCGCTTAAGACCAACATTGCCACTTTGGGACCTATTGTGCTTCCTATAAGCGAGCAGCTTCTTTTCTTTTCATCACTTGTGGTTAAGAAGCTGTTCAAGTCTAACGTCAAGCCATATATCCCGGATTTCAAGCTGGCATTTGATCATTTTTGCATACACGCCGGAAGGATCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

316

Amino Acids

35.43

Weight (kDa)

9.39

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 1 - 288 2.3e-145 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 238
Acc36I ACCTGC 1 cut(s) 238
AccB1I GGYRCC 1 cut(s) 67
AccI GTMKAC 1 cut(s) 21
AccII CGCG 2 cut(s) 593, 674
AclWI GGATC 1 cut(s) 947
AcoI YGGCCR 2 cut(s) 212, 222
AcyI GRCGYC 1 cut(s) 347
AfaI GTAC 5 cut(s) 74, 257, 380, 508, 638
AfeI AGCGCT 1 cut(s) 155
AfiI CCNNNNNNNGG 4 cut(s) 106, 169, 231, 621
AflII CTTAAG 1 cut(s) 776
AgsI TTSAA 2 cut(s) 868, 901
AhdI GACNNNNNGTC 1 cut(s) 121
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 6 cut(s) 333, 446, 643, 829, 862, 904
AluI AGCT 6 cut(s) 333, 446, 643, 829, 862, 904
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 1 cut(s) 167
AlwI GGATC 1 cut(s) 947
Ama87I CYCGRG 2 cut(s) 128, 149
Aor51HI AGCGCT 1 cut(s) 155
AoxI GGCC 3 cut(s) 160, 212, 222
ApeKI GCWGC 2 cut(s) 330, 826
Asp700I GAANNNNTTC 1 cut(s) 863
AspLEI GCGC 3 cut(s) 156, 674, 775
AspS9I GGNCC 2 cut(s) 660, 800
AsuC2I CCSGG 3 cut(s) 185, 211, 893
AsuHPI GGTGA 3 cut(s) 50, 202, 262
AvaI CYCGRG 2 cut(s) 128, 149
AvaII GGWCC 2 cut(s) 660, 800
BanI GGYRCC 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 65, 97
Bbv12I GWGCWC 1 cut(s) 93
BbvI GCAGC 2 cut(s) 342, 838
BccI CCATC 4 cut(s) 214, 486, 672, 748
BceAI ACGGC 2 cut(s) 199, 334
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BciT130I CCWGG 1 cut(s) 232
BclI TGATCA 1 cut(s) 913
BcnI CCSGG 3 cut(s) 185, 211, 893
BcoDI GTCTC 1 cut(s) 167
BfoI RGCGCY 2 cut(s) 157, 776
BfrI CTTAAG 1 cut(s) 776
BfuAI ACCTGC 1 cut(s) 238
BglI GCCNNNNNGGC 1 cut(s) 770
BisI GCNGC 4 cut(s) 225, 228, 331, 827
BlsI GCNGC 4 cut(s) 226, 229, 332, 828
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 4 cut(s) 185, 211, 232, 893
Bme18I GGWCC 2 cut(s) 660, 800
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 2 cut(s) 128, 149
BmgT120I GGNCC 2 cut(s) 660, 800
BmiI GGNNCC 4 cut(s) 69, 589, 662, 801
BmrFI CCNGG 4 cut(s) 185, 211, 232, 893
BmsI GCATC 1 cut(s) 183
BoxI GACNNNNGTC 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 3 cut(s) 185, 211, 893
BsaHI GRCGYC 1 cut(s) 347
BsaJI CCNNGG 4 cut(s) 163, 209, 231, 591
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 169, 231, 621
Bse118I RCCGGY 1 cut(s) 764
Bse1I ACTGG 1 cut(s) 733
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 4 cut(s) 163, 209, 231, 591
BseGI GGATG 2 cut(s) 201, 718
BseLI CCNNNNNNNGG 4 cut(s) 106, 169, 231, 621
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 2 cut(s) 501, 536
BseNI ACTGG 1 cut(s) 733
BseRI GAGGAG 2 cut(s) 185, 251
BseX3I CGGCCG 1 cut(s) 212
BseXI GCAGC 2 cut(s) 342, 838
BseYI CCCAGC 1 cut(s) 434
Bsh1236I CGCG 2 cut(s) 593, 674
Bsh1285I CGRYCG 1 cut(s) 215
BshFI GGCC 3 cut(s) 162, 214, 224
BshNI GGYRCC 1 cut(s) 67
BsiEI CGRYCG 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 93
BsiHKCI CYCGRG 2 cut(s) 128, 149
BsiSI CCGG 6 cut(s) 159, 185, 211, 765, 893, 933
BslFI GGGAC 2 cut(s) 167, 813
BslI CCNNNNNNNGG 4 cut(s) 106, 169, 231, 621
BsmAI GTCTC 1 cut(s) 167
BsmFI GGGAC 2 cut(s) 167, 813
BsnI GGCC 3 cut(s) 162, 214, 224
BsoBI CYCGRG 2 cut(s) 128, 149
Bsp1286I GDGCHC 1 cut(s) 93
Bsp143I GATC 4 cut(s) 55, 451, 913, 939
BspANI GGCC 3 cut(s) 162, 214, 224
BspCNI CTCAG 2 cut(s) 502, 535
BspFNI CGCG 2 cut(s) 593, 674
BspLI GGNNCC 4 cut(s) 69, 589, 662, 801
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 1 cut(s) 947
BspT107I GGYRCC 1 cut(s) 67
BspTI CTTAAG 1 cut(s) 776
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 1 cut(s) 764
BsrI ACTGG 1 cut(s) 733
BssAI RCCGGY 1 cut(s) 764
BssECI CCNNGG 4 cut(s) 163, 209, 231, 591
BssMI GATC 4 cut(s) 55, 451, 913, 939
BssNI GRCGYC 1 cut(s) 347
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 2 cut(s) 72, 260
BstACI GRCGYC 1 cut(s) 347
BstAFI CTTAAG 1 cut(s) 776
BstC8I GCNNGC 5 cut(s) 40, 50, 630, 824, 906
BstDEI CTNAG 3 cut(s) 510, 522, 633
BstDSI CCRYGG 1 cut(s) 591
BstENI CCTNNNNNAGG 2 cut(s) 104, 167
BstF5I GGATG 2 cut(s) 201, 718
BstFNI CGCG 2 cut(s) 593, 674
BstH2I RGCGCY 2 cut(s) 157, 776
BstHHI GCGC 3 cut(s) 156, 674, 775
BstKTI GATC 4 cut(s) 58, 454, 916, 942
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 4 cut(s) 55, 451, 913, 939
BstMCI CGRYCG 1 cut(s) 215
BstMWI GCNNNNNNNGC 1 cut(s) 770
BstNI CCWGG 1 cut(s) 232
BstPAI GACNNNNGTC 1 cut(s) 179
BstSCI CCNGG 4 cut(s) 183, 209, 230, 891
BstUI CGCG 2 cut(s) 593, 674
BstV1I GCAGC 2 cut(s) 342, 838
BstZI CGGCCG 1 cut(s) 212
BsuRI GGCC 3 cut(s) 162, 214, 224
BtgI CCRYGG 1 cut(s) 591
BtsCI GGATG 2 cut(s) 201, 718
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 5 cut(s) 40, 50, 630, 824, 906
CfoI GCGC 3 cut(s) 156, 674, 775
Cfr10I RCCGGY 1 cut(s) 764
Cfr13I GGNCC 2 cut(s) 660, 800
Cfr42I CCGCGG 1 cut(s) 594
CseI GACGC 1 cut(s) 355
Csp6I GTAC 5 cut(s) 73, 256, 379, 507, 637
CviAII CATG 4 cut(s) 85, 290, 364, 650
CviQI GTAC 5 cut(s) 73, 256, 379, 507, 637
DdeI CTNAG 3 cut(s) 510, 522, 633
DpnI GATC 4 cut(s) 57, 453, 915, 941
DpnII GATC 4 cut(s) 55, 451, 913, 939
DriI GACNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 2 cut(s) 212, 222
EagI CGGCCG 1 cut(s) 212
Eam1105I GACNNNNNGTC 1 cut(s) 121
EciI GGCGGA 2 cut(s) 257, 349
EclXI CGGCCG 1 cut(s) 212
Eco47I GGWCC 2 cut(s) 660, 800
Eco47III AGCGCT 1 cut(s) 155
Eco52I CGGCCG 1 cut(s) 212
Eco88I CYCGRG 2 cut(s) 128, 149
EcoNI CCTNNNNNAGG 2 cut(s) 104, 167
EcoO109I RGGNCCY 2 cut(s) 660, 800
EcoRII CCWGG 1 cut(s) 230
EcoT22I ATGCAT 1 cut(s) 198
FaeI CATG 4 cut(s) 88, 293, 367, 653
FaqI GGGAC 2 cut(s) 167, 813
FatI CATG 4 cut(s) 84, 289, 363, 649
FauI CCCGC 3 cut(s) 57, 104, 621
FbaI TGATCA 1 cut(s) 913
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 4 cut(s) 225, 228, 331, 827
FokI GGATG 2 cut(s) 188, 725
Fsp4HI GCNGC 4 cut(s) 225, 228, 331, 827
GlaI GCGC 3 cut(s) 155, 673, 774
GluI GCNGC 4 cut(s) 225, 228, 331, 827
GsaI CCCAGC 1 cut(s) 438
HaeII RGCGCY 2 cut(s) 157, 776
HaeIII GGCC 3 cut(s) 162, 214, 224
HapII CCGG 6 cut(s) 159, 185, 211, 765, 893, 933
HgaI GACGC 1 cut(s) 355
HhaI GCGC 3 cut(s) 156, 674, 775
Hin1I GRCGYC 1 cut(s) 347
Hin1II CATG 4 cut(s) 88, 293, 367, 653
Hin6I GCGC 3 cut(s) 154, 672, 773
HinP1I GCGC 3 cut(s) 154, 672, 773
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 3 cut(s) 119, 145, 313
HpaII CCGG 6 cut(s) 159, 185, 211, 765, 893, 933
HphI GGTGA 3 cut(s) 50, 202, 262
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 3 cut(s) 345, 483, 525
Hpy188III TCNNGA 2 cut(s) 149, 704
Hpy8I GTNNAC 1 cut(s) 22
Hpy99I CGWCG 5 cut(s) 119, 127, 220, 349, 355
HpyAV CCTTC 1 cut(s) 930
HpyCH4III ACNGT 2 cut(s) 72, 260
HpyCH4IV ACGT 1 cut(s) 876
HpyCH4V TGCA 4 cut(s) 196, 330, 472, 924
HpyF10VI GCNNNNNNNGC 1 cut(s) 770
HpyF3I CTNAG 3 cut(s) 510, 522, 633
HpySE526I ACGT 1 cut(s) 876
Hsp92I GRCGYC 1 cut(s) 347
Hsp92II CATG 4 cut(s) 88, 293, 367, 653
HspAI GCGC 3 cut(s) 154, 672, 773
Ksp22I TGATCA 1 cut(s) 913
KspI CCGCGG 1 cut(s) 594
Kzo9I GATC 4 cut(s) 55, 451, 913, 939
LmnI GCTCC 4 cut(s) 88, 161, 244, 706
Lsp1109I GCAGC 2 cut(s) 342, 838
LweI GCATC 1 cut(s) 183
MaeII ACGT 1 cut(s) 876
MalI GATC 4 cut(s) 57, 453, 915, 941
MboI GATC 4 cut(s) 55, 451, 913, 939
MfeI CAATTG 2 cut(s) 565, 759
MhlI GDGCHC 1 cut(s) 93
MluCI AATT 5 cut(s) 325, 383, 526, 565, 759
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 368
Mph1103I ATGCAT 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 863
MseI TTAA 5 cut(s) 339, 372, 408, 777, 855
MspA1I CMGCKG 1 cut(s) 593
MspCI CTTAAG 1 cut(s) 776
MspI CCGG 6 cut(s) 159, 185, 211, 765, 893, 933
MspR9I CCNGG 4 cut(s) 185, 211, 232, 893
MunI CAATTG 2 cut(s) 565, 759
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 2 cut(s) 593, 674
MwoI GCNNNNNNNGC 1 cut(s) 770
NciI CCSGG 3 cut(s) 185, 211, 893
NdeII GATC 4 cut(s) 55, 451, 913, 939
NlaIII CATG 4 cut(s) 88, 293, 367, 653
NlaIV GGNNCC 4 cut(s) 69, 589, 662, 801
NmeAIII GCCGAG 1 cut(s) 144
NsiI ATGCAT 1 cut(s) 198
PaeR7I CTCGAG 2 cut(s) 128, 149
PaqCI CACCTGC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 863
PfeI GAWTC 2 cut(s) 145, 313
PflFI GACNNNGTC 1 cut(s) 349
PfoI TCCNGGA 2 cut(s) 183, 891
PkrI GCNGC 4 cut(s) 226, 229, 332, 828
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PpuMI RGGWCCY 2 cut(s) 660, 800
PshAI GACNNNNGTC 1 cut(s) 179
Psp5II RGGWCCY 2 cut(s) 660, 800
Psp6I CCWGG 1 cut(s) 230
PspFI CCCAGC 1 cut(s) 434
PspGI CCWGG 1 cut(s) 230
PspN4I GGNNCC 4 cut(s) 69, 589, 662, 801
PspPI GGNCC 2 cut(s) 660, 800
PspPPI RGGWCCY 2 cut(s) 660, 800
PspXI VCTCGAGB 1 cut(s) 128
PsyI GACNNNGTC 1 cut(s) 349
RsaI GTAC 5 cut(s) 74, 257, 380, 508, 638
RsaNI GTAC 5 cut(s) 73, 256, 379, 507, 637
SacII CCGCGG 1 cut(s) 594
SaqAI TTAA 5 cut(s) 339, 372, 408, 777, 855
SatI GCNGC 4 cut(s) 225, 228, 331, 827
Sau3AI GATC 4 cut(s) 55, 451, 913, 939
Sau96I GGNCC 2 cut(s) 660, 800
ScaI AGTACT 1 cut(s) 508
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 4 cut(s) 185, 211, 232, 893
SduI GDGCHC 1 cut(s) 93
SfaNI GCATC 1 cut(s) 183
Sfr274I CTCGAG 2 cut(s) 128, 149
Sfr303I CCGCGG 1 cut(s) 594
SgrBI CCGCGG 1 cut(s) 594
SinI GGWCC 2 cut(s) 660, 800
SlaI CTCGAG 2 cut(s) 128, 149
SmlI CTYRAG 4 cut(s) 62, 128, 149, 776
SmoI CTYRAG 4 cut(s) 62, 128, 149, 776
Sse9I AATT 5 cut(s) 325, 383, 526, 565, 759
SspI AATATT 1 cut(s) 397
StyD4I CCNGG 4 cut(s) 183, 209, 230, 891
TaaI ACNGT 2 cut(s) 72, 260
TaiI ACGT 1 cut(s) 879
TaqI TCGA 7 cut(s) 30, 114, 129, 150, 218, 743, 942
TasI AATT 5 cut(s) 325, 383, 526, 565, 759
TatI WGTACW 3 cut(s) 378, 506, 636
TauI GCSGC 2 cut(s) 227, 230
TfiI GAWTC 2 cut(s) 145, 313
Tru1I TTAA 5 cut(s) 339, 372, 408, 777, 855
Tru9I TTAA 5 cut(s) 339, 372, 408, 777, 855
TseI GCWGC 2 cut(s) 330, 826
TspDTI ATGAA 3 cut(s) 73, 638, 831
TspGWI ACGGA 2 cut(s) 604, 680
Tth111I GACNNNGTC 1 cut(s) 349
Vha464I CTTAAG 1 cut(s) 776
VpaK11BI GGWCC 2 cut(s) 660, 800
XagI CCTNNNNNAGG 2 cut(s) 104, 167
XhoI CTCGAG 2 cut(s) 128, 149
XmiI GTMKAC 1 cut(s) 21
XmnI GAANNNNTTC 1 cut(s) 863
ZrmI AGTACT 1 cut(s) 508
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.