MD00G1059300.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0010704
Physical Location & Seq
Reverse (-)
11028711 .. 11029106
396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1059300.v1.1.491

Sequence Viewer

Length: 396 bp
ATGTTGATACCCAATTGCTTGTTTCGTGTTGGGGGTTCCGCGGTTTTGCTTTCCAATAAGTCCGTTGATAGGAGGCGGGCTAAGTACAAGCTTGTTCATGTTGTGAGGACCCATCGTGGCGCGAATGATAAAGTTTTCCGTTGTGTTTATCAGGAGCAGGATGACAAGGGGAAGACTGGTGTGTCTTTGTCGAAAGATTTGATGGCAATTGCCGGTGGGGCGCTTAAGACCAACATTGCCACTTTGGGACCTATTGTGCTTCCTATAAGCGAGCAGCTTCTTTTCTTTTCATCACTTGTGGTTAAGAAGCTGTTCAAGTCCAACGTCAAGCCATATATCCCGGATTTCAAGCTGGCATTTGATCATTTTTGCATACACGCTGGAAGGATCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

132

Amino Acids

14.57

Weight (kDa)

9.92

Isoelectric Point (pI)

20.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 1 - 104 3.1e-47 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
AccII CGCG 2 cut(s) 41, 122
AciI CCGC 3 cut(s) 39, 41, 76
AclWI GGATC 1 cut(s) 395
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 69
AflII CTTAAG 1 cut(s) 224
AgsI TTSAA 2 cut(s) 316, 349
AluBI AGCT 4 cut(s) 91, 277, 310, 352
AluI AGCT 4 cut(s) 91, 277, 310, 352
AlwI GGATC 1 cut(s) 395
ApeKI GCWGC 1 cut(s) 274
Asp700I GAANNNNTTC 1 cut(s) 311
AspLEI GCGC 2 cut(s) 122, 223
AspS9I GGNCC 2 cut(s) 108, 248
AsuC2I CCSGG 1 cut(s) 341
AvaII GGWCC 2 cut(s) 108, 248
BbsI GAAGAC 1 cut(s) 179
BbvI GCAGC 1 cut(s) 286
BccI CCATC 2 cut(s) 120, 196
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 1 cut(s) 341
BfoI RGCGCY 1 cut(s) 224
BfrI CTTAAG 1 cut(s) 224
BglI GCCNNNNNGGC 1 cut(s) 218
BisI GCNGC 1 cut(s) 275
BlsI GCNGC 1 cut(s) 276
Bme1390I CCNGG 1 cut(s) 341
Bme18I GGWCC 2 cut(s) 108, 248
BmgT120I GGNCC 2 cut(s) 108, 248
BmiI GGNNCC 3 cut(s) 37, 110, 249
BmrFI CCNGG 1 cut(s) 341
BpiI GAAGAC 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 181
Bse3DI GCAATG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMI GCAATG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 181
BseXI GCAGC 1 cut(s) 286
Bsh1236I CGCG 2 cut(s) 41, 122
BsiSI CCGG 2 cut(s) 213, 341
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 261
Bsp143I GATC 2 cut(s) 361, 387
BspACI CCGC 3 cut(s) 39, 41, 76
BspFNI CGCG 2 cut(s) 41, 122
BspLI GGNNCC 3 cut(s) 37, 110, 249
BspPI GGATC 1 cut(s) 395
BspTI CTTAAG 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 2 cut(s) 361, 387
BstAFI CTTAAG 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 78, 272, 354
BstDEI CTNAG 1 cut(s) 81
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 166
BstFNI CGCG 2 cut(s) 41, 122
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 2 cut(s) 122, 223
BstKTI GATC 2 cut(s) 364, 390
BstMBI GATC 2 cut(s) 361, 387
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 339
BstUI CGCG 2 cut(s) 41, 122
BstV1I GCAGC 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 179
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 166
Cac8I GCNNGC 3 cut(s) 78, 272, 354
CfoI GCGC 2 cut(s) 122, 223
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 108, 248
Cfr42I CCGCGG 1 cut(s) 42
Csp6I GTAC 1 cut(s) 85
CviAII CATG 1 cut(s) 98
CviJI RGCY 6 cut(s) 80, 91, 277, 310, 331, 352
CviKI_1 RGCY 6 cut(s) 80, 91, 277, 310, 331, 352
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 81
DpnI GATC 2 cut(s) 363, 389
DpnII GATC 2 cut(s) 361, 387
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
Eco47I GGWCC 2 cut(s) 108, 248
EcoO109I RGGNCCY 2 cut(s) 108, 248
FaeI CATG 1 cut(s) 101
FaiI YATR 5 cut(s) 99, 266, 334, 336, 374
FaqI GGGAC 1 cut(s) 261
FatI CATG 1 cut(s) 97
FauI CCCGC 1 cut(s) 69
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 1 cut(s) 275
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 275
GlaI GCGC 2 cut(s) 121, 222
GluI GCNGC 1 cut(s) 275
HaeII RGCGCY 1 cut(s) 224
HapII CCGG 2 cut(s) 213, 341
HhaI GCGC 2 cut(s) 122, 223
Hin1II CATG 1 cut(s) 101
Hin6I GCGC 2 cut(s) 120, 221
HinP1I GCGC 2 cut(s) 120, 221
HindIII AAGCTT 1 cut(s) 89
HpaII CCGG 2 cut(s) 213, 341
Hpy188III TCNNGA 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 378
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 1 cut(s) 372
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 1 cut(s) 81
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 1 cut(s) 101
HspAI GCGC 2 cut(s) 120, 221
Ksp22I TGATCA 1 cut(s) 361
KspI CCGCGG 1 cut(s) 42
Kzo9I GATC 2 cut(s) 361, 387
LmnI GCTCC 1 cut(s) 154
LpnPI CCDG 7 cut(s) 137, 143, 162, 226, 338, 354, 366
Lsp1109I GCAGC 1 cut(s) 286
MaeII ACGT 1 cut(s) 324
MalI GATC 2 cut(s) 363, 389
MboI GATC 2 cut(s) 361, 387
MboII GAAGA 1 cut(s) 184
MfeI CAATTG 2 cut(s) 13, 207
MluCI AATT 2 cut(s) 13, 207
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 2 cut(s) 66, 99
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 2 cut(s) 225, 303
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 224
MspI CCGG 2 cut(s) 213, 341
MspR9I CCNGG 1 cut(s) 341
MunI CAATTG 2 cut(s) 13, 207
MvnI CGCG 2 cut(s) 41, 122
MwoI GCNNNNNNNGC 1 cut(s) 218
NciI CCSGG 1 cut(s) 341
NdeII GATC 2 cut(s) 361, 387
NlaIII CATG 1 cut(s) 101
NlaIV GGNNCC 3 cut(s) 37, 110, 249
PdmI GAANNNNTTC 1 cut(s) 311
PfoI TCCNGGA 1 cut(s) 339
PkrI GCNGC 1 cut(s) 276
PpuMI RGGWCCY 2 cut(s) 108, 248
Psp5II RGGWCCY 2 cut(s) 108, 248
PspN4I GGNNCC 3 cut(s) 37, 110, 249
PspPI GGNCC 2 cut(s) 108, 248
PspPPI RGGWCCY 2 cut(s) 108, 248
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SacII CCGCGG 1 cut(s) 42
SaqAI TTAA 2 cut(s) 225, 303
SatI GCNGC 1 cut(s) 275
Sau3AI GATC 2 cut(s) 361, 387
Sau96I GGNCC 2 cut(s) 108, 248
ScrFI CCNGG 1 cut(s) 341
SetI ASST 6 cut(s) 93, 253, 279, 312, 327, 354
Sfr303I CCGCGG 1 cut(s) 42
SgrBI CCGCGG 1 cut(s) 42
SinI GGWCC 2 cut(s) 108, 248
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 2 cut(s) 13, 207
SsiI CCGC 3 cut(s) 39, 41, 76
StyD4I CCNGG 1 cut(s) 339
TaiI ACGT 1 cut(s) 327
TaqI TCGA 2 cut(s) 191, 390
TasI AATT 2 cut(s) 13, 207
TatI WGTACW 1 cut(s) 84
Tru1I TTAA 2 cut(s) 225, 303
Tru9I TTAA 2 cut(s) 225, 303
TseI GCWGC 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 86, 279
TspGWI ACGGA 2 cut(s) 52, 128
Vha464I CTTAAG 1 cut(s) 224
VpaK11BI GGWCC 2 cut(s) 108, 248
XmnI GAANNNNTTC 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.