pycom15g02250

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010704
Physical Location & Seq
Reverse (-)
1339537 .. 1341087
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g02250.1

Sequence Viewer

Length: 1551 bp
ATGGACCCAGGCGGCGCAACCGATGTCACCACCGCGCCGGGAGGAGACGGTCAGGTCGGGGTCCAGATCCACCAAAACCGACGGCTTCCCGATTTCCTCCAGAGCGTTAATCTCAAGTACGTGAAATTAGGGTACCATTACTTGATCTCTAATCTCTTAACCCTCTGCTTCATCCCGTTGATTATCGTAACCCTAATCGAAGCCTCCCAAATGGACATTTACGACATTCAACAACTCTGGCTCCACCTCCAGTACAATCTCGTCTCCGTCATCATCTGCTCCGCCGTCCTCGTCTTCGGGTTGACCGTCTACATCATGACCCGACCCAGACCCGTTTACTTGGTCGATTACGCCTGCTACCGCCCGCCCGATCACCTCAAGGCCCCTTACCAGCGCTTCATGGAGCACTCCCGCCTCACCCAGGACTTCGACGACTCGTCGCTAGAGTTCCAGCGCAAGATTCTCGAGCGGTCCGGCCTCGGCGAGGAGACTTATGTCCCGGAGGCGATGCATTACATCCCTCCCCGGCCTTCGATGGCCGCCGCCAGGGATGAGGCGGAGCAGGTGATGTATGGTGCTTTGGACAATCTGTTCGCCAATACCCATGTGAAGCCGAAGGACATTGGGATTCTCGTTGTGAATTGCAGCTTGTTTAATCCGACGCCGTCGCTTTCCGCCATGATTGTTAATAAATACAAATTGAGGGGTAATATTAGGAGTTTCAATTTGGGGGGAATGGGTTGCAGTGCTGGGGTCATAGCTGTTGATCTTGCTAAGGACTTGTTGCAAATCCATAGGAACACTTATGCGGTTGTTGTTAGCACTGAGAACATTACTCAGAATTGGTATTTTGGGAATAAGAAGTCTATGTTGATACCCAATTGCTTGTTTCGGGTTGGGGGTTCTGCAGTTTTGCTTTCCAACAAGTCCGTGGATAGGAGACGGGCTAAGTACAAGCTTGTTCATGTCGTGAGGACACATCGTGGCGCGGATGATAAAGCTTTTCGTTGTGTTTACCAGGAGCAGGATGATAAGGGGAAGACTGGTGTGTCCTTGTCCAAAGATTTGATGGCAATTGCCGGTGGGACGCTTAAGACCAACATTACCACTTTGGGACCTATTGTGCTTCCGATAAGCGAGCAGCTTCTTTTCTTTTCTTCAATTGTGGTTAACAAGCTGTTCAAGTCGAATGTCAAGCCTTATATCCCGGATTTCAAGCTGGCGTTTGATCATTTTTGCATACATGCCGGGGGAAGGGCTGTGATTGATGAGCTGGAGAAGAACCTGCAGTTGCTGCCTATACATGTGGAGGCTTCTCGGATGACTCTACACCGGTTTGGGAATACTTCGTCGAGCTCCATTTGGTATGAACTGGCGTATATGGAGGCCAAGGGGAGGATGCGCAAGGGCAACCGTGTCTGGCAGATTGCCTTTGGAAGTGGTTTTAAATGTAACAGTGCGGTCTGGGAGTCGCTTCGGAATGTGAAGCCATCTCGTAATGGTCCTTGGGAAGACTGCATTGACAAGTATCCTGTGAAAGTAGTTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

517

Amino Acids

58.03

Weight (kDa)

9.13

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 104 - 392 1.3e-145 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 410 - 490 1.8e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 412 - 488 1.1e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 553
AasI GACNNNNNNGTC 2 cut(s) 53, 1048
Acc16I TGCGCA 1 cut(s) 1403
Acc36I ACCTGC 2 cut(s) 553, 1293
Acc65I GGTACC 1 cut(s) 132
AccB1I GGYRCC 1 cut(s) 132
AccBSI CCGCTC 1 cut(s) 469
AccI GTMKAC 1 cut(s) 309
AccII CGCG 2 cut(s) 35, 989
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 537
AcyI GRCGYC 1 cut(s) 662
AdeI CACNNNGTG 1 cut(s) 983
AfaI GTAC 4 cut(s) 119, 134, 254, 953
AfeI AGCGCT 1 cut(s) 395
AfiI CCNNNNNNNGG 7 cut(s) 421, 484, 546, 936, 1024, 1254, 1395
AflII CTTAAG 1 cut(s) 1091
AflIII ACRYGT 1 cut(s) 1303
AgeI ACCGGT 1 cut(s) 1332
AgsI TTSAA 5 cut(s) 230, 724, 1161, 1183, 1216
AhdI GACNNNNNGTC 1 cut(s) 436
AjnI CCWGG 4 cut(s) 7, 420, 545, 1017
AloI GAACNNNNNNTCC 2 cut(s) 575, 607
AluBI AGCT 9 cut(s) 648, 761, 958, 1001, 1144, 1177, 1219, 1273, 1356
AluI AGCT 9 cut(s) 648, 761, 958, 1001, 1144, 1177, 1219, 1273, 1356
Alw21I GWGCWC 2 cut(s) 408, 1358
Alw26I GTCTC 4 cut(s) 39, 268, 482, 934
AlwI GGATC 1 cut(s) 61
AlwNI CAGNNNCTG 1 cut(s) 1294
Ama87I CYCGRG 1 cut(s) 464
Aor51HI AGCGCT 1 cut(s) 395
AoxI GGCC 5 cut(s) 381, 475, 527, 537, 1386
ApeKI GCWGC 3 cut(s) 645, 1141, 1294
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AsiGI ACCGGT 1 cut(s) 1332
Asp718I GGTACC 1 cut(s) 132
AspLEI GCGC 6 cut(s) 17, 37, 396, 456, 989, 1404
AspS9I GGNCC 6 cut(s) 4, 61, 382, 471, 1115, 1502
AsuC2I CCSGG 5 cut(s) 39, 500, 526, 1208, 1249
AsuHPI GGTGA 4 cut(s) 19, 365, 409, 577
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 5 cut(s) 4, 61, 471, 1115, 1502
BanI GGYRCC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 1358
BbsI GAAGAC 3 cut(s) 286, 1046, 1518
Bbv12I GWGCWC 2 cut(s) 408, 1358
BbvI GCAGC 3 cut(s) 657, 1153, 1281
BccI CCATC 3 cut(s) 529, 1063, 1498
BceAI ACGGC 3 cut(s) 98, 269, 649
BcgI CGANNNNNNTGC 2 cut(s) 445, 479
BciT130I CCWGG 4 cut(s) 9, 422, 547, 1019
BciVI GTATCC 1 cut(s) 1539
BclI TGATCA 1 cut(s) 1228
BcnI CCSGG 5 cut(s) 39, 500, 526, 1208, 1249
BcoDI GTCTC 4 cut(s) 39, 268, 482, 934
BfaI CTAG 2 cut(s) 443, 1549
BfmI CTRYAG 2 cut(s) 906, 1286
BfoI RGCGCY 1 cut(s) 397
BfrI CTTAAG 1 cut(s) 1091
BfuAI ACCTGC 2 cut(s) 553, 1293
BfuI GTATCC 1 cut(s) 1539
BisI GCNGC 6 cut(s) 13, 540, 543, 646, 1142, 1295
BlsI GCNGC 6 cut(s) 14, 541, 544, 647, 1143, 1296
Bme1390I CCNGG 9 cut(s) 9, 39, 422, 500, 526, 547, 1019, 1208, 1249
Bme18I GGWCC 5 cut(s) 4, 61, 471, 1115, 1502
BmeRI GACNNNNNGTC 1 cut(s) 436
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 6 cut(s) 4, 61, 382, 471, 1115, 1502
BmiI GGNNCC 6 cut(s) 6, 62, 134, 242, 384, 1116
BmrFI CCNGG 9 cut(s) 9, 39, 422, 500, 526, 547, 1019, 1208, 1249
BmsI GCATC 2 cut(s) 498, 1389
BoxI GACNNNNGTC 1 cut(s) 494
BpiI GAAGAC 3 cut(s) 286, 1046, 1518
BpmI CTGGAG 3 cut(s) 83, 233, 1295
Bpu10I CCTNAGC 1 cut(s) 774
BpuEI CTTGAG 2 cut(s) 98, 362
BpuMI CCSGG 5 cut(s) 39, 500, 526, 1208, 1249
BsaAI YACGTR 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 662
BsaJI CCNNGG 9 cut(s) 7, 420, 478, 524, 546, 930, 1248, 1389, 1505
BsaWI WCCGGW 1 cut(s) 1332
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 7 cut(s) 421, 484, 546, 936, 1024, 1254, 1395
Bse118I RCCGGY 2 cut(s) 1079, 1332
Bse1I ACTGG 3 cut(s) 250, 1048, 1377
BseBI CCWGG 4 cut(s) 9, 422, 547, 1019
BseDI CCNNGG 9 cut(s) 7, 420, 478, 524, 546, 930, 1248, 1389, 1505
BseGI GGATG 7 cut(s) 171, 516, 556, 997, 1033, 1326, 1404
BseLI CCNNNNNNNGG 7 cut(s) 421, 484, 546, 936, 1024, 1254, 1395
BseMII CTCAG 2 cut(s) 816, 851
BseNI ACTGG 3 cut(s) 250, 1048, 1377
BseRI GAGGAG 2 cut(s) 57, 500
BseXI GCAGC 3 cut(s) 657, 1153, 1281
BseYI CCCAGC 1 cut(s) 749
Bsh1236I CGCG 2 cut(s) 35, 989
BshFI GGCC 5 cut(s) 383, 477, 529, 539, 1388
BshNI GGYRCC 1 cut(s) 132
BshTI ACCGGT 1 cut(s) 1332
BsiHKAI GWGCWC 2 cut(s) 408, 1358
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 8 cut(s) 38, 474, 500, 526, 1080, 1208, 1248, 1333
BslFI GGGAC 3 cut(s) 482, 1099, 1128
BslI CCNNNNNNNGG 7 cut(s) 421, 484, 546, 936, 1024, 1254, 1395
BsmAI GTCTC 4 cut(s) 39, 268, 482, 934
BsmBI CGTCTC 3 cut(s) 39, 268, 934
BsmFI GGGAC 3 cut(s) 482, 1099, 1128
BsnI GGCC 5 cut(s) 383, 477, 529, 539, 1388
BsoBI CYCGRG 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 408, 1358
Bsp143I GATC 5 cut(s) 66, 144, 370, 766, 1228
BspANI GGCC 5 cut(s) 383, 477, 529, 539, 1388
BspCNI CTCAG 2 cut(s) 817, 850
BspFNI CGCG 2 cut(s) 35, 989
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 6 cut(s) 6, 62, 134, 242, 384, 1116
BspMAI CTGCAG 2 cut(s) 910, 1290
BspMI ACCTGC 2 cut(s) 553, 1293
BspPI GGATC 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 132
BspTI CTTAAG 1 cut(s) 1091
BsrBI CCGCTC 1 cut(s) 469
BsrFI RCCGGY 2 cut(s) 1079, 1332
BsrI ACTGG 3 cut(s) 250, 1048, 1377
BssAI RCCGGY 2 cut(s) 1079, 1332
BssECI CCNNGG 9 cut(s) 7, 420, 478, 524, 546, 930, 1248, 1389, 1505
BssMI GATC 5 cut(s) 66, 144, 370, 766, 1228
BssNI GRCGYC 1 cut(s) 662
BssT1I CCWWGG 2 cut(s) 1389, 1505
Bst2UI CCWGG 4 cut(s) 9, 422, 547, 1019
Bst4CI ACNGT 4 cut(s) 50, 307, 1415, 1457
BstACI GRCGYC 1 cut(s) 662
BstAFI CTTAAG 1 cut(s) 1091
BstAPI GCANNNNNTGC 1 cut(s) 1294
BstBAI YACGTR 1 cut(s) 121
BstC8I GCNNGC 4 cut(s) 355, 365, 1139, 1221
BstDEI CTNAG 4 cut(s) 774, 825, 837, 948
BstDSI CCRYGG 1 cut(s) 930
BstENI CCTNNNNNAGG 2 cut(s) 419, 482
BstF5I GGATG 7 cut(s) 171, 516, 556, 997, 1033, 1326, 1404
BstFNI CGCG 2 cut(s) 35, 989
BstH2I RGCGCY 1 cut(s) 397
BstHHI GCGC 6 cut(s) 17, 37, 396, 456, 989, 1404
BstKTI GATC 5 cut(s) 69, 147, 373, 769, 1231
BstMAI GTCTC 4 cut(s) 39, 268, 482, 934
BstMBI GATC 5 cut(s) 66, 144, 370, 766, 1228
BstMWI GCNNNNNNNGC 1 cut(s) 1294
BstNI CCWGG 4 cut(s) 9, 422, 547, 1019
BstNSI RCATGY 2 cut(s) 1247, 1307
BstPAI GACNNNNGTC 1 cut(s) 494
BstSCI CCNGG 9 cut(s) 7, 37, 420, 498, 524, 545, 1017, 1206, 1247
BstSFI CTRYAG 2 cut(s) 906, 1286
BstUI CGCG 2 cut(s) 35, 989
BstV1I GCAGC 3 cut(s) 657, 1153, 1281
BstV2I GAAGAC 3 cut(s) 286, 1046, 1518
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuI GTATCC 1 cut(s) 1539
BsuRI GGCC 5 cut(s) 383, 477, 529, 539, 1388
BtgI CCRYGG 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 521
BtsCI GGATG 7 cut(s) 171, 516, 556, 997, 1033, 1326, 1404
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 3 cut(s) 751, 822, 1462
BveI ACCTGC 2 cut(s) 553, 1293
Cac8I GCNNGC 4 cut(s) 355, 365, 1139, 1221
CaiI CAGNNNCTG 1 cut(s) 1294
CciI TCATGA 1 cut(s) 315
CfoI GCGC 6 cut(s) 17, 37, 396, 456, 989, 1404
Cfr10I RCCGGY 2 cut(s) 1079, 1332
Cfr13I GGNCC 6 cut(s) 4, 61, 382, 471, 1115, 1502
CpoI CGGWCCG 1 cut(s) 471
CseI GACGC 2 cut(s) 670, 1096
Csp6I GTAC 4 cut(s) 118, 133, 253, 952
CspAI ACCGGT 1 cut(s) 1332
CspI CGGWCCG 1 cut(s) 471
CviAII CATG 7 cut(s) 316, 400, 605, 679, 965, 1244, 1304
CviQI GTAC 4 cut(s) 118, 133, 253, 952
DdeI CTNAG 4 cut(s) 774, 825, 837, 948
DpnI GATC 5 cut(s) 68, 146, 372, 768, 1230
DpnII GATC 5 cut(s) 66, 144, 370, 766, 1228
DraI TTTAAA 1 cut(s) 1447
DraIII CACNNNGTG 1 cut(s) 983
DrdI GACNNNNNNGTC 2 cut(s) 53, 1048
DriI GACNNNNNGTC 1 cut(s) 436
DseDI GACNNNNNNGTC 2 cut(s) 53, 1048
EaeI YGGCCR 1 cut(s) 537
Eam1105I GACNNNNNGTC 1 cut(s) 436
EciI GGCGGA 3 cut(s) 271, 572, 664
Ecl136II GAGCTC 1 cut(s) 1356
Eco130I CCWWGG 2 cut(s) 1389, 1505
Eco24I GRGCYC 1 cut(s) 1358
Eco47I GGWCC 5 cut(s) 4, 61, 471, 1115, 1502
Eco47III AGCGCT 1 cut(s) 395
Eco53kI GAGCTC 1 cut(s) 1356
Eco88I CYCGRG 1 cut(s) 464
EcoICRI GAGCTC 1 cut(s) 1356
EcoNI CCTNNNNNAGG 2 cut(s) 419, 482
EcoO109I RGGNCCY 2 cut(s) 382, 1115
EcoRII CCWGG 4 cut(s) 7, 420, 545, 1017
EcoT14I CCWWGG 2 cut(s) 1389, 1505
EcoT22I ATGCAT 1 cut(s) 513
EcoT38I GRGCYC 1 cut(s) 1358
ErhI CCWWGG 2 cut(s) 1389, 1505
Esp3I CGTCTC 3 cut(s) 39, 268, 934
FaeI CATG 7 cut(s) 319, 403, 608, 682, 968, 1247, 1307
FaqI GGGAC 3 cut(s) 482, 1099, 1128
FatI CATG 7 cut(s) 315, 399, 604, 678, 964, 1243, 1303
FauI CCCGC 2 cut(s) 372, 419
FbaI TGATCA 1 cut(s) 1228
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 6 cut(s) 13, 540, 543, 646, 1142, 1295
FokI GGATG 7 cut(s) 158, 503, 563, 1004, 1040, 1333, 1411
FriOI GRGCYC 1 cut(s) 1358
Fsp4HI GCNGC 6 cut(s) 13, 540, 543, 646, 1142, 1295
FspBI CTAG 2 cut(s) 443, 1549
FspI TGCGCA 1 cut(s) 1403
GlaI GCGC 6 cut(s) 16, 36, 395, 455, 988, 1403
GluI GCNGC 6 cut(s) 13, 540, 543, 646, 1142, 1295
GsaI CCCAGC 1 cut(s) 753
GsuI CTGGAG 3 cut(s) 83, 233, 1295
HaeII RGCGCY 1 cut(s) 397
HaeIII GGCC 5 cut(s) 383, 477, 529, 539, 1388
HapII CCGG 8 cut(s) 38, 474, 500, 526, 1080, 1208, 1248, 1333
HgaI GACGC 2 cut(s) 670, 1096
HhaI GCGC 6 cut(s) 17, 37, 396, 456, 989, 1404
Hin1I GRCGYC 1 cut(s) 662
Hin1II CATG 7 cut(s) 319, 403, 608, 682, 968, 1247, 1307
Hin6I GCGC 6 cut(s) 15, 35, 394, 454, 987, 1402
HinP1I GCGC 6 cut(s) 15, 35, 394, 454, 987, 1402
HincII GTYRAC 2 cut(s) 303, 1171
HindII GTYRAC 2 cut(s) 303, 1171
HindIII AAGCTT 2 cut(s) 956, 999
HinfI GANTC 5 cut(s) 434, 460, 628, 1324, 1469
HpaI GTTAAC 1 cut(s) 1171
HpaII CCGG 8 cut(s) 38, 474, 500, 526, 1080, 1208, 1248, 1333
HphI GGTGA 4 cut(s) 19, 365, 409, 577
Hpy166II GTNNAC 5 cut(s) 303, 310, 337, 1015, 1171
Hpy188I TCNGA 5 cut(s) 660, 840, 1131, 1320, 1479
Hpy188III TCNNGA 6 cut(s) 64, 89, 100, 316, 464, 970
Hpy8I GTNNAC 5 cut(s) 303, 310, 337, 1015, 1171
Hpy99I CGWCG 6 cut(s) 84, 434, 442, 664, 670, 1354
HpyAV CCTTC 3 cut(s) 540, 610, 1248
HpyCH4III ACNGT 4 cut(s) 50, 307, 1415, 1457
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 8 cut(s) 511, 645, 744, 787, 908, 1239, 1288, 1518
HpyF10VI GCNNNNNNNGC 1 cut(s) 1294
HpyF3I CTNAG 4 cut(s) 774, 825, 837, 948
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 662
Hsp92II CATG 7 cut(s) 319, 403, 608, 682, 968, 1247, 1307
HspAI GCGC 6 cut(s) 15, 35, 394, 454, 987, 1402
KpnI GGTACC 1 cut(s) 136
Ksp22I TGATCA 1 cut(s) 1228
KspAI GTTAAC 1 cut(s) 1171
Kzo9I GATC 5 cut(s) 66, 144, 370, 766, 1228
LmnI GCTCC 6 cut(s) 246, 284, 403, 559, 1021, 1361
Lsp1109I GCAGC 3 cut(s) 657, 1153, 1281
LweI GCATC 2 cut(s) 498, 1389
MaeI CTAG 2 cut(s) 443, 1549
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 3 cut(s) 25, 187, 1451
MalI GATC 5 cut(s) 68, 146, 372, 768, 1230
MbiI CCGCTC 1 cut(s) 469
MboI GATC 5 cut(s) 66, 144, 370, 766, 1228
MboII GAAGA 5 cut(s) 286, 1051, 1149, 1291, 1523
MfeI CAATTG 3 cut(s) 880, 1074, 1161
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 2 cut(s) 408, 1358
MluCI AATT 8 cut(s) 125, 640, 698, 724, 841, 880, 1074, 1161
MlyI GAGTC 3 cut(s) 428, 1318, 1478
MmeI TCCRAC 2 cut(s) 683, 945
Mph1103I ATGCAT 1 cut(s) 513
MseI TTAA 7 cut(s) 108, 158, 654, 687, 1092, 1170, 1446
MspCI CTTAAG 1 cut(s) 1091
MspI CCGG 8 cut(s) 38, 474, 500, 526, 1080, 1208, 1248, 1333
MspR9I CCNGG 9 cut(s) 9, 39, 422, 500, 526, 547, 1019, 1208, 1249
MunI CAATTG 3 cut(s) 880, 1074, 1161
MvaI CCWGG 4 cut(s) 9, 422, 547, 1019
MvnI CGCG 2 cut(s) 35, 989
MwoI GCNNNNNNNGC 1 cut(s) 1294
NciI CCSGG 5 cut(s) 39, 500, 526, 1208, 1249
NdeII GATC 5 cut(s) 66, 144, 370, 766, 1228
NlaIII CATG 7 cut(s) 319, 403, 608, 682, 968, 1247, 1307
NlaIV GGNNCC 6 cut(s) 6, 62, 134, 242, 384, 1116
NmeAIII GCCGAG 1 cut(s) 459
NmuCI GTSAC 1 cut(s) 25
NsbI TGCGCA 1 cut(s) 1403
NsiI ATGCAT 1 cut(s) 513
NspI RCATGY 2 cut(s) 1247, 1307
PaeR7I CTCGAG 1 cut(s) 464
PagI TCATGA 1 cut(s) 315
PaqCI CACCTGC 1 cut(s) 553
PciI ACATGT 1 cut(s) 1303
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 2 cut(s) 460, 628
PflFI GACNNNGTC 1 cut(s) 664
PfoI TCCNGGA 2 cut(s) 498, 1206
PinAI ACCGGT 1 cut(s) 1332
PkrI GCNGC 6 cut(s) 14, 541, 544, 647, 1143, 1296
PleI GAGTC 3 cut(s) 428, 1318, 1477
PpsI GAGTC 3 cut(s) 428, 1318, 1477
Ppu21I YACGTR 1 cut(s) 121
PpuMI RGGWCCY 1 cut(s) 1115
PscI ACATGT 1 cut(s) 1303
PshAI GACNNNNGTC 1 cut(s) 494
Psp124BI GAGCTC 1 cut(s) 1358
Psp5II RGGWCCY 1 cut(s) 1115
Psp6I CCWGG 4 cut(s) 7, 420, 545, 1017
PspFI CCCAGC 1 cut(s) 749
PspGI CCWGG 4 cut(s) 7, 420, 545, 1017
PspN4I GGNNCC 6 cut(s) 6, 62, 134, 242, 384, 1116
PspPI GGNCC 6 cut(s) 4, 61, 382, 471, 1115, 1502
PspPPI RGGWCCY 1 cut(s) 1115
PstI CTGCAG 2 cut(s) 910, 1290
PstNI CAGNNNCTG 1 cut(s) 1294
PsuI RGATCY 1 cut(s) 66
PsyI GACNNNGTC 1 cut(s) 664
RsaI GTAC 4 cut(s) 119, 134, 254, 953
RsaNI GTAC 4 cut(s) 118, 133, 253, 952
Rsr2I CGGWCCG 1 cut(s) 471
RsrII CGGWCCG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 1358
SaqAI TTAA 7 cut(s) 108, 158, 654, 687, 1092, 1170, 1446
SatI GCNGC 6 cut(s) 13, 540, 543, 646, 1142, 1295
Sau3AI GATC 5 cut(s) 66, 144, 370, 766, 1228
Sau96I GGNCC 6 cut(s) 4, 61, 382, 471, 1115, 1502
SchI GAGTC 3 cut(s) 428, 1318, 1478
ScrFI CCNGG 9 cut(s) 9, 39, 422, 500, 526, 547, 1019, 1208, 1249
SduI GDGCHC 2 cut(s) 408, 1358
SfaNI GCATC 2 cut(s) 498, 1389
SfcI CTRYAG 2 cut(s) 906, 1286
Sfr274I CTCGAG 1 cut(s) 464
SinI GGWCC 5 cut(s) 4, 61, 471, 1115, 1502
SlaI CTCGAG 1 cut(s) 464
SmlI CTYRAG 4 cut(s) 113, 377, 464, 1091
SmoI CTYRAG 4 cut(s) 113, 377, 464, 1091
Sse9I AATT 8 cut(s) 125, 640, 698, 724, 841, 880, 1074, 1161
SspI AATATT 1 cut(s) 712
SspMI CTAG 2 cut(s) 443, 1549
SstI GAGCTC 1 cut(s) 1358
StyD4I CCNGG 9 cut(s) 7, 37, 420, 498, 524, 545, 1017, 1206, 1247
StyI CCWWGG 2 cut(s) 1389, 1505
TaaI ACNGT 4 cut(s) 50, 307, 1415, 1457
TaiI ACGT 1 cut(s) 123
TaqI TCGA 7 cut(s) 198, 345, 429, 465, 533, 1187, 1352
TasI AATT 8 cut(s) 125, 640, 698, 724, 841, 880, 1074, 1161
TatI WGTACW 2 cut(s) 252, 951
TauI GCSGC 3 cut(s) 15, 542, 545
TfiI GAWTC 2 cut(s) 460, 628
Tru1I TTAA 7 cut(s) 108, 158, 654, 687, 1092, 1170, 1446
Tru9I TTAA 7 cut(s) 108, 158, 654, 687, 1092, 1170, 1446
TscAI CASTG 3 cut(s) 751, 829, 1462
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 3 cut(s) 645, 1141, 1294
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 4 cut(s) 160, 388, 953, 1383
TspGWI ACGGA 2 cut(s) 256, 919
TspRI CASTG 3 cut(s) 751, 829, 1462
Tth111I GACNNNGTC 1 cut(s) 664
Vha464I CTTAAG 1 cut(s) 1091
VpaK11BI GGWCC 5 cut(s) 4, 61, 471, 1115, 1502
XagI CCTNNNNNAGG 2 cut(s) 419, 482
XceI RCATGY 2 cut(s) 1247, 1307
XcmI CCANNNNNNNNNTGG 2 cut(s) 928, 1066
XhoI CTCGAG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 2 cut(s) 443, 1549
Zsp2I ATGCAT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.