MD00G1059400.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0010704
Physical Location & Seq
Reverse (-)
11029185 .. 11029682
498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1059400.v1.1.491

Sequence Viewer

Length: 498 bp
ATGTTCGGGTTGATCGTCTACATCATGACCCGACCCAGACCCGTCTACTTGGTCGATTACGCCTGCTACCGCCCGCCCGATCACCTCAAGGCACCGTACCAGCGCTTCATGGAGCACTCCCGCCTCACGCAGGACTTCGACGACTCGTCGCTCGAGTTCCAGCGCAAGATTCTCGAGCACTCCGGCCTCGGCGAGGAGACTTATGTCCCAGAGGCGATGCATTACATCCCTCCCCGGCCGTCGATGGCCGCCGCCAGGGAGGAGGCGGAGCAGGTGATGTACGGTGCTCTGGATAATCTGTTTGCCAATACCCATGTGAAGCCAAAGGACATTGGGATTCTCGTTGTGAATTGCAGCTTATTTAATCTGACGCAGTCGCTTTCCGCCATGATTGTTAATAAGTACAAATTGAGGGGTAATATTAGAAGTTTTAACTTAGGGGGAATGGGTTGTAGTGCTGGGGTTATAGCTGTTGATCTTGCCAAAGACTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

166

Amino Acids

18.65

Weight (kDa)

6.51

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 7 - 165 9.5e-72 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 262
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 91
AccI GTMKAC 2 cut(s) 18, 45
AciI CCGC 7 cut(s) 70, 74, 121, 249, 252, 266, 384
AcoI YGGCCR 2 cut(s) 236, 246
AfaI GTAC 3 cut(s) 98, 281, 404
AfeI AGCGCT 1 cut(s) 104
AfiI CCNNNNNNNGG 3 cut(s) 130, 193, 255
AhdI GACNNNNNGTC 1 cut(s) 145
AjnI CCWGG 1 cut(s) 254
AluBI AGCT 2 cut(s) 357, 470
AluI AGCT 2 cut(s) 357, 470
Alw21I GWGCWC 3 cut(s) 117, 180, 289
Alw26I GTCTC 1 cut(s) 191
Ama87I CYCGRG 2 cut(s) 152, 173
Aor51HI AGCGCT 1 cut(s) 104
AoxI GGCC 3 cut(s) 184, 236, 246
ApeKI GCWGC 1 cut(s) 354
AspLEI GCGC 2 cut(s) 105, 165
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 74, 286
AvaI CYCGRG 2 cut(s) 152, 173
BanI GGYRCC 1 cut(s) 91
BarI GAAGNNNNNNTAC 2 cut(s) 89, 121
Bbv12I GWGCWC 3 cut(s) 117, 180, 289
BbvI GCAGC 1 cut(s) 366
BccI CCATC 1 cut(s) 238
BceAI ACGGC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 154, 188
BciT130I CCWGG 1 cut(s) 256
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 106
BfuAI ACCTGC 1 cut(s) 262
BisI GCNGC 3 cut(s) 249, 252, 355
BlsI GCNGC 3 cut(s) 250, 253, 356
Bme1390I CCNGG 2 cut(s) 235, 256
BmeRI GACNNNNNGTC 1 cut(s) 145
BmeT110I CYCGRG 2 cut(s) 152, 173
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 2 cut(s) 235, 256
BmsI GCATC 1 cut(s) 207
BoxI GACNNNNGTC 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 235
BsaJI CCNNGG 3 cut(s) 187, 233, 255
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 193, 255
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 3 cut(s) 187, 233, 255
BseGI GGATG 1 cut(s) 225
BseLI CCNNNNNNNGG 3 cut(s) 130, 193, 255
BseRI GAGGAG 2 cut(s) 209, 275
BseX3I CGGCCG 1 cut(s) 236
BseXI GCAGC 1 cut(s) 366
BseYI CCCAGC 1 cut(s) 458
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 3 cut(s) 186, 238, 248
BshNI GGYRCC 1 cut(s) 91
BsiEI CGRYCG 1 cut(s) 239
BsiHKAI GWGCWC 3 cut(s) 117, 180, 289
BsiHKCI CYCGRG 2 cut(s) 152, 173
BsiSI CCGG 2 cut(s) 183, 235
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 3 cut(s) 130, 193, 255
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 191
BsnI GGCC 3 cut(s) 186, 238, 248
BsoBI CYCGRG 2 cut(s) 152, 173
Bsp1286I GDGCHC 3 cut(s) 117, 180, 289
Bsp143I GATC 3 cut(s) 12, 79, 475
BspACI CCGC 7 cut(s) 70, 74, 121, 249, 252, 266, 384
BspANI GGCC 3 cut(s) 186, 238, 248
BspHI TCATGA 1 cut(s) 24
BspLI GGNNCC 1 cut(s) 93
BspMI ACCTGC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 91
BssECI CCNNGG 3 cut(s) 187, 233, 255
BssMI GATC 3 cut(s) 12, 79, 475
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 2 cut(s) 96, 284
BstC8I GCNNGC 2 cut(s) 64, 74
BstDEI CTNAG 1 cut(s) 436
BstENI CCTNNNNNAGG 2 cut(s) 128, 191
BstF5I GGATG 1 cut(s) 225
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 2 cut(s) 105, 165
BstKTI GATC 3 cut(s) 15, 82, 478
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 3 cut(s) 12, 79, 475
BstMCI CGRYCG 1 cut(s) 239
BstNI CCWGG 1 cut(s) 256
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 2 cut(s) 233, 254
BstV1I GCAGC 1 cut(s) 366
BstZI CGGCCG 1 cut(s) 236
BsuRI GGCC 3 cut(s) 186, 238, 248
BtgZI GCGATG 1 cut(s) 230
BtsCI GGATG 1 cut(s) 225
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 2 cut(s) 64, 74
CciI TCATGA 1 cut(s) 24
CfoI GCGC 2 cut(s) 105, 165
CseI GACGC 1 cut(s) 379
Csp6I GTAC 3 cut(s) 97, 280, 403
CviAII CATG 4 cut(s) 25, 109, 314, 388
CviJI RGCY 6 cut(s) 186, 238, 248, 322, 357, 470
CviKI_1 RGCY 6 cut(s) 186, 238, 248, 322, 357, 470
CviQI GTAC 3 cut(s) 97, 280, 403
DdeI CTNAG 1 cut(s) 436
DpnI GATC 3 cut(s) 14, 81, 477
DpnII GATC 3 cut(s) 12, 79, 475
DriI GACNNNNNGTC 1 cut(s) 145
EaeI YGGCCR 2 cut(s) 236, 246
EagI CGGCCG 1 cut(s) 236
Eam1105I GACNNNNNGTC 1 cut(s) 145
EciI GGCGGA 2 cut(s) 281, 373
EclXI CGGCCG 1 cut(s) 236
Eco47III AGCGCT 1 cut(s) 104
Eco52I CGGCCG 1 cut(s) 236
Eco88I CYCGRG 2 cut(s) 152, 173
EcoNI CCTNNNNNAGG 2 cut(s) 128, 191
EcoRII CCWGG 1 cut(s) 254
EcoT22I ATGCAT 1 cut(s) 222
FaeI CATG 4 cut(s) 28, 112, 317, 391
FaiI YATR 6 cut(s) 26, 110, 204, 315, 389, 467
FaqI GGGAC 1 cut(s) 191
FatI CATG 4 cut(s) 24, 108, 313, 387
FauI CCCGC 2 cut(s) 81, 128
FblI GTMKAC 2 cut(s) 18, 45
Fnu4HI GCNGC 3 cut(s) 249, 252, 355
FokI GGATG 1 cut(s) 212
Fsp4HI GCNGC 3 cut(s) 249, 252, 355
GlaI GCGC 2 cut(s) 104, 164
GluI GCNGC 3 cut(s) 249, 252, 355
GsaI CCCAGC 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 106
HaeIII GGCC 3 cut(s) 186, 238, 248
HapII CCGG 2 cut(s) 183, 235
HgaI GACGC 1 cut(s) 379
HhaI GCGC 2 cut(s) 105, 165
Hin1II CATG 4 cut(s) 28, 112, 317, 391
Hin6I GCGC 2 cut(s) 103, 163
HinP1I GCGC 2 cut(s) 103, 163
HinfI GANTC 3 cut(s) 143, 169, 337
HpaII CCGG 2 cut(s) 183, 235
HphI GGTGA 2 cut(s) 74, 286
Hpy166II GTNNAC 2 cut(s) 19, 46
Hpy188I TCNGA 1 cut(s) 369
Hpy188III TCNNGA 3 cut(s) 25, 173, 290
Hpy8I GTNNAC 2 cut(s) 19, 46
Hpy99I CGWCG 3 cut(s) 143, 151, 244
HpyCH4III ACNGT 2 cut(s) 96, 284
HpyCH4V TGCA 2 cut(s) 220, 354
HpyF3I CTNAG 1 cut(s) 436
Hsp92II CATG 4 cut(s) 28, 112, 317, 391
HspAI GCGC 2 cut(s) 103, 163
Kzo9I GATC 3 cut(s) 12, 79, 475
LmnI GCTCC 2 cut(s) 112, 268
Lsp1109I GCAGC 1 cut(s) 366
LweI GCATC 1 cut(s) 207
MalI GATC 3 cut(s) 14, 81, 477
MboI GATC 3 cut(s) 12, 79, 475
MhlI GDGCHC 3 cut(s) 117, 180, 289
MluCI AATT 2 cut(s) 349, 407
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 9 cut(s) 95, 134, 187, 197, 205, 240, 253, 256, 405
Mph1103I ATGCAT 1 cut(s) 222
MseI TTAA 3 cut(s) 363, 396, 432
MspI CCGG 2 cut(s) 183, 235
MspR9I CCNGG 2 cut(s) 235, 256
MvaI CCWGG 1 cut(s) 256
NciI CCSGG 1 cut(s) 235
NdeII GATC 3 cut(s) 12, 79, 475
NlaIII CATG 4 cut(s) 28, 112, 317, 391
NlaIV GGNNCC 1 cut(s) 93
NmeAIII GCCGAG 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 222
PaeR7I CTCGAG 2 cut(s) 152, 173
PagI TCATGA 1 cut(s) 24
PaqCI CACCTGC 1 cut(s) 262
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PfeI GAWTC 2 cut(s) 169, 337
PflFI GACNNNGTC 1 cut(s) 373
PkrI GCNGC 3 cut(s) 250, 253, 356
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PshAI GACNNNNGTC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 458
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 93
PspXI VCTCGAGB 1 cut(s) 152
PsyI GACNNNGTC 1 cut(s) 373
RsaI GTAC 3 cut(s) 98, 281, 404
RsaNI GTAC 3 cut(s) 97, 280, 403
SaqAI TTAA 3 cut(s) 363, 396, 432
SatI GCNGC 3 cut(s) 249, 252, 355
Sau3AI GATC 3 cut(s) 12, 79, 475
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 235, 256
SduI GDGCHC 3 cut(s) 117, 180, 289
SetI ASST 4 cut(s) 87, 276, 359, 472
SfaNI GCATC 1 cut(s) 207
Sfr274I CTCGAG 2 cut(s) 152, 173
SlaI CTCGAG 2 cut(s) 152, 173
SmlI CTYRAG 3 cut(s) 86, 152, 173
SmoI CTYRAG 3 cut(s) 86, 152, 173
Sse9I AATT 2 cut(s) 349, 407
SsiI CCGC 7 cut(s) 70, 74, 121, 249, 252, 266, 384
SspI AATATT 1 cut(s) 421
StyD4I CCNGG 2 cut(s) 233, 254
TaaI ACNGT 2 cut(s) 96, 284
TaqI TCGA 5 cut(s) 54, 138, 153, 174, 242
TasI AATT 2 cut(s) 349, 407
TatI WGTACW 1 cut(s) 402
TauI GCSGC 2 cut(s) 251, 254
TfiI GAWTC 2 cut(s) 169, 337
Tru1I TTAA 3 cut(s) 363, 396, 432
Tru9I TTAA 3 cut(s) 363, 396, 432
TseI GCWGC 1 cut(s) 354
TspDTI ATGAA 1 cut(s) 97
Tth111I GACNNNGTC 1 cut(s) 373
XagI CCTNNNNNAGG 2 cut(s) 128, 191
XhoI CTCGAG 2 cut(s) 152, 173
XmiI GTMKAC 2 cut(s) 18, 45
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.