Rroxscaffold_172G00445500

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000172
OG0010704
Physical Location & Seq
Reverse (-)
42151 .. 43710
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_172G00445500.1

Sequence Viewer

Length: 1560 bp
ATGGACCAAGGCGGCCCAGCCCACGTCGCCGCCACGGCCGGCGGCGGAGGAGGAGGAGGTCAGGTCGGGGTTCGGATCCACCAAGCCCGAAGGCTGCCGGATTTCCTCCAGAGTGTGAATCTCAAGTACGTGAAATTAGGCTACCATTATCTCATCTCCAATCTCTTGACCTTGTGCTTCATCCCCTTAATCATCGTCACCTTAATCGAGGCCTCCCAAATGGACCTGCACGACCTCCGCCACCTCTGGCTCCACCTCCAGTACAACCTCGTCAGCGTCATCATCTGCTCCGCCGTCCTCGTCTTCGGGTTGACCGTTTACATCATGACCCGGCCCAGGCCCATTTACCTGGTCGATTACTCCTGCTACCGCCCTCCCGACCACCTCAAGGCCCCCTACCAGCGCTTCATGGACCACTCCCGCCTCACCAACGACTTCGACGAGTCCTCGCTCGAGTTCCAGCGCAAGATTCTGGAGCGCTCCGGCCTCGGAGAAGAGACCTACGTCCCCGAAGCGATGCACCACATCCCTCCCCGGCCGTCCATGGCGGCGGCGAGGGAAGAGGCCGAGCAGGTTATGTATGGCGCCCTGGATAATCTGTTTGCAAGTACCAATGTGAAGCCCAAGGATATTGGGATTCTTGTTGTCAACTGCAGCTTGTTTAACCCGACGCCATCGCTGTCGGCGATGATTGTGAATAAGTATAGGCTGAGGGGGAACATTAGGAGCTTCAATTTGGGAGGGATGGGTTGCAGTGCTGGGGTTATAGCTGTTGATCTTGCTAAGGACTTGTTGCAGGTTCATAGGAATACTTATGCTGTTGTGGTCAGTACTGAGAACATTACTCAGAATTGGTATTTTGGGAATAAGAAGTCTATGTTGATACCCAATTGCTTGTTTCGAGTTGGGGGCTCGGCGGTTTTGCTGTCCAATAAGTCTGTGGATAGGAGGAGGGCCAAGTACAGGCTTGTTCATGTTGTGAGGACTCATCGCGGTGCGGATGACAAGGCGTTTCGGTGTGTTTATCAGGAGCAGGATGATAAGGGGAAGACTGGTGTTTCTTTGTCCAAAGATTTGATGGCCATTGCCGGTGGGGCGCTCAAGACTAACATAACTACTTTGGGACCTATTGTGCTTCCGATAAGCGAGCAGCTGCTTTTCTTTTCTTCTCTTGTGGTTAAGAAGCTGTTGAATGGGGATGTCAAGCCGTATATCCCGGATTTCAAGCTGGCATTTGATCATTTCTGCATACATGCTGGGGGAAGGGCTGTGATTGATGAGCTGGAGAAGAACCTGCAGTTGTTGCCAATACATGTGGAGGCTTCTCGGATGACTCTACACCGGTTTGGGAATACTTCCTCGAGCTCCATTTGGTATGAGTTGGCCTATACAGAGGCCAAAGGGAGGATGCGCAAGGGCAACCGCGTCTGGCAGATTGCCTTTGGTAGTGGTTTCAAATGTAACAGTGCAGTCTGGGAGGCTCTGCGGAATGTGAAGCCATCTCATAATGGTCCTTGGGAGGACAGCATTGACAAGTACCCCGTCAAAGTAGTTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

519

Amino Acids

57.96

Weight (kDa)

9.3

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 107 - 396 1.1e-145 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 413 - 493 3.4e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 415 - 486 1.1e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1412
Acc36I ACCTGC 4 cut(s) 234, 562, 787, 1302
AccB1I GGYRCC 1 cut(s) 584
AccII CGCG 2 cut(s) 993, 1425
AclWI GGATC 2 cut(s) 70, 83
AcoI YGGCCR 3 cut(s) 36, 536, 1080
AcyI GRCGYC 2 cut(s) 585, 671
AfaI GTAC 6 cut(s) 128, 263, 610, 832, 962, 1538
AfeI AGCGCT 2 cut(s) 404, 479
AfiI CCNNNNNNNGG 4 cut(s) 336, 388, 963, 1404
AflIII ACRYGT 1 cut(s) 1312
AgeI ACCGGT 1 cut(s) 1341
AgsI TTSAA 4 cut(s) 733, 1192, 1225, 1456
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 3 cut(s) 335, 348, 588
AluBI AGCT 8 cut(s) 657, 729, 770, 1153, 1186, 1228, 1282, 1365
AluI AGCT 8 cut(s) 657, 729, 770, 1153, 1186, 1228, 1282, 1365
Alw21I GWGCWC 1 cut(s) 1367
Alw26I GTCTC 1 cut(s) 491
AlwI GGATC 2 cut(s) 70, 83
Ama87I CYCGRG 2 cut(s) 452, 1360
Aor51HI AGCGCT 2 cut(s) 404, 479
ApeKI GCWGC 4 cut(s) 94, 654, 1150, 1153
AsiGI ACCGGT 1 cut(s) 1341
AspLEI GCGC 6 cut(s) 405, 465, 480, 587, 1099, 1413
AsuC2I CCSGG 3 cut(s) 331, 535, 1217
AsuHPI GGTGA 2 cut(s) 190, 418
AvaI CYCGRG 2 cut(s) 452, 1360
AvaII GGWCC 5 cut(s) 4, 223, 412, 1124, 1511
BalI TGGCCA 1 cut(s) 1082
BamHI GGATCC 1 cut(s) 75
BanI GGYRCC 1 cut(s) 584
BanII GRGCYC 2 cut(s) 914, 1367
BbsI GAAGAC 2 cut(s) 295, 1055
Bbv12I GWGCWC 1 cut(s) 1367
BbvCI CCTCAGC 1 cut(s) 710
BbvI GCAGC 4 cut(s) 81, 666, 1140, 1162
BccI CCATC 4 cut(s) 682, 739, 1072, 1507
BceAI ACGGC 4 cut(s) 51, 278, 523, 1192
BciT130I CCWGG 3 cut(s) 337, 350, 590
BclI TGATCA 1 cut(s) 1237
BcnI CCSGG 3 cut(s) 331, 535, 1217
BcoDI GTCTC 1 cut(s) 491
BfmI CTRYAG 2 cut(s) 652, 1295
BfoI RGCGCY 4 cut(s) 406, 481, 588, 1100
BfuAI ACCTGC 4 cut(s) 234, 562, 787, 1302
BglI GCCNNNNNGGC 2 cut(s) 35, 1094
BisI GCNGC 9 cut(s) 13, 30, 43, 95, 549, 552, 655, 1151, 1154
BlsI GCNGC 9 cut(s) 14, 31, 44, 96, 550, 553, 656, 1152, 1155
BmcAI AGTACT 1 cut(s) 832
Bme1390I CCNGG 6 cut(s) 331, 337, 350, 535, 590, 1217
Bme18I GGWCC 5 cut(s) 4, 223, 412, 1124, 1511
BmeT110I CYCGRG 2 cut(s) 452, 1360
BmgBI CACGTC 1 cut(s) 25
BmiI GGNNCC 5 cut(s) 77, 251, 393, 586, 1125
BmrFI CCNGG 6 cut(s) 331, 337, 350, 535, 590, 1217
BmsI GCATC 2 cut(s) 507, 1398
BoxI GACNNNNGTC 1 cut(s) 503
BpiI GAAGAC 2 cut(s) 295, 1055
BpmI CTGGAG 4 cut(s) 92, 242, 494, 1304
Bpu10I CCTNAGC 2 cut(s) 710, 783
BpuEI CTTGAG 3 cut(s) 107, 371, 1085
BpuMI CCSGG 3 cut(s) 331, 535, 1217
BsaAI YACGTR 1 cut(s) 130
BsaHI GRCGYC 2 cut(s) 585, 671
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 9 cut(s) 7, 33, 335, 487, 533, 543, 588, 624, 1514
BsaWI WCCGGW 1 cut(s) 1341
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 4 cut(s) 336, 388, 963, 1404
Bse118I RCCGGY 3 cut(s) 38, 1088, 1341
Bse1I ACTGG 2 cut(s) 259, 1057
Bse3DI GCAATG 1 cut(s) 1083
BseBI CCWGG 3 cut(s) 337, 350, 590
BseDI CCNNGG 9 cut(s) 7, 33, 335, 487, 533, 543, 588, 624, 1514
BseGI GGATG 8 cut(s) 180, 525, 750, 1006, 1042, 1204, 1335, 1413
BseLI CCNNNNNNNGG 4 cut(s) 336, 388, 963, 1404
BseMI GCAATG 1 cut(s) 1083
BseMII CTCAG 3 cut(s) 701, 825, 860
BseNI ACTGG 2 cut(s) 259, 1057
BseRI GAGGAG 4 cut(s) 63, 66, 69, 964
BseX3I CGGCCG 2 cut(s) 36, 536
BseXI GCAGC 4 cut(s) 81, 666, 1140, 1162
BseYI CCCAGC 3 cut(s) 16, 758, 1256
BsgI GTGCAG 2 cut(s) 212, 1488
Bsh1236I CGCG 2 cut(s) 993, 1425
Bsh1285I CGRYCG 2 cut(s) 39, 539
BshNI GGYRCC 1 cut(s) 584
BshTI ACCGGT 1 cut(s) 1341
BsiEI CGRYCG 2 cut(s) 39, 539
BsiHKAI GWGCWC 1 cut(s) 1367
BsiHKCI CYCGRG 2 cut(s) 452, 1360
BsiSI CCGG 8 cut(s) 39, 98, 331, 483, 535, 1089, 1217, 1342
BslFI GGGAC 2 cut(s) 491, 1137
BslI CCNNNNNNNGG 4 cut(s) 336, 388, 963, 1404
BsmAI GTCTC 1 cut(s) 491
BsmFI GGGAC 2 cut(s) 491, 1137
Bso31I GGTCTC 1 cut(s) 491
BsoBI CYCGRG 2 cut(s) 452, 1360
Bsp1286I GDGCHC 2 cut(s) 914, 1367
Bsp143I GATC 3 cut(s) 75, 775, 1237
Bsp19I CCATGG 1 cut(s) 543
BspCNI CTCAG 3 cut(s) 702, 826, 859
BspFNI CGCG 2 cut(s) 993, 1425
BspHI TCATGA 1 cut(s) 324
BspLI GGNNCC 5 cut(s) 77, 251, 393, 586, 1125
BspMAI CTGCAG 2 cut(s) 656, 1299
BspMI ACCTGC 4 cut(s) 234, 562, 787, 1302
BspPI GGATC 2 cut(s) 70, 83
BspT107I GGYRCC 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 1083
BsrFI RCCGGY 3 cut(s) 38, 1088, 1341
BsrI ACTGG 2 cut(s) 259, 1057
BssAI RCCGGY 3 cut(s) 38, 1088, 1341
BssECI CCNNGG 9 cut(s) 7, 33, 335, 487, 533, 543, 588, 624, 1514
BssMI GATC 3 cut(s) 75, 775, 1237
BssNI GRCGYC 2 cut(s) 585, 671
BssT1I CCWWGG 4 cut(s) 7, 543, 624, 1514
Bst2UI CCWGG 3 cut(s) 337, 350, 590
Bst4CI ACNGT 2 cut(s) 316, 1466
Bst6I CTCTTC 2 cut(s) 489, 555
BstACI GRCGYC 2 cut(s) 585, 671
BstAPI GCANNNNNTGC 1 cut(s) 1303
BstBAI YACGTR 1 cut(s) 130
BstC8I GCNNGC 3 cut(s) 40, 1148, 1230
BstDEI CTNAG 4 cut(s) 710, 783, 834, 846
BstDSI CCRYGG 2 cut(s) 33, 543
BstF5I GGATG 8 cut(s) 180, 525, 750, 1006, 1042, 1204, 1335, 1413
BstFNI CGCG 2 cut(s) 993, 1425
BstH2I RGCGCY 4 cut(s) 406, 481, 588, 1100
BstHHI GCGC 6 cut(s) 405, 465, 480, 587, 1099, 1413
BstKTI GATC 3 cut(s) 78, 778, 1240
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 3 cut(s) 75, 775, 1237
BstMCI CGRYCG 2 cut(s) 39, 539
BstMWI GCNNNNNNNGC 4 cut(s) 26, 35, 1094, 1303
BstNI CCWGG 3 cut(s) 337, 350, 590
BstNSI RCATGY 2 cut(s) 1256, 1316
BstPAI GACNNNNGTC 1 cut(s) 503
BstSCI CCNGG 6 cut(s) 329, 335, 348, 533, 588, 1215
BstSFI CTRYAG 2 cut(s) 652, 1295
BstUI CGCG 2 cut(s) 993, 1425
BstV1I GCAGC 4 cut(s) 81, 666, 1140, 1162
BstV2I GAAGAC 2 cut(s) 295, 1055
BstX2I RGATCY 1 cut(s) 75
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 1 cut(s) 75
BstZI CGGCCG 2 cut(s) 36, 536
BtgI CCRYGG 2 cut(s) 33, 543
BtgZI GCGATG 4 cut(s) 530, 660, 701, 974
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 8 cut(s) 180, 525, 750, 1006, 1042, 1204, 1335, 1413
BtsI GCAGTG 1 cut(s) 760
BtsIMutI CAGTG 2 cut(s) 760, 1471
BveI ACCTGC 4 cut(s) 234, 562, 787, 1302
Cac8I GCNNGC 3 cut(s) 40, 1148, 1230
CciI TCATGA 1 cut(s) 324
CfoI GCGC 6 cut(s) 405, 465, 480, 587, 1099, 1413
Cfr10I RCCGGY 3 cut(s) 38, 1088, 1341
CseI GACGC 3 cut(s) 265, 679, 1414
CsiI ACCWGGT 1 cut(s) 348
Csp6I GTAC 6 cut(s) 127, 262, 609, 831, 961, 1537
CspAI ACCGGT 1 cut(s) 1341
CspCI CAANNNNNGTGG 2 cut(s) 1296, 1331
CviAII CATG 6 cut(s) 325, 409, 544, 974, 1253, 1313
CviQI GTAC 6 cut(s) 127, 262, 609, 831, 961, 1537
DdeI CTNAG 4 cut(s) 710, 783, 834, 846
DinI GGCGCC 1 cut(s) 586
DpnI GATC 3 cut(s) 77, 777, 1239
DpnII GATC 3 cut(s) 75, 775, 1237
EaeI YGGCCR 3 cut(s) 36, 536, 1080
EagI CGGCCG 2 cut(s) 36, 536
Eam1104I CTCTTC 2 cut(s) 489, 555
EarI CTCTTC 2 cut(s) 489, 555
EciI GGCGGA 3 cut(s) 60, 227, 280
Ecl136II GAGCTC 1 cut(s) 1365
EclXI CGGCCG 2 cut(s) 36, 536
Eco130I CCWWGG 4 cut(s) 7, 543, 624, 1514
Eco147I AGGCCT 1 cut(s) 212
Eco24I GRGCYC 2 cut(s) 914, 1367
Eco31I GGTCTC 1 cut(s) 491
Eco47I GGWCC 5 cut(s) 4, 223, 412, 1124, 1511
Eco47III AGCGCT 2 cut(s) 404, 479
Eco52I CGGCCG 2 cut(s) 36, 536
Eco53kI GAGCTC 1 cut(s) 1365
Eco88I CYCGRG 2 cut(s) 452, 1360
EcoICRI GAGCTC 1 cut(s) 1365
EcoO109I RGGNCCY 2 cut(s) 391, 1124
EcoRII CCWGG 3 cut(s) 335, 348, 588
EcoT14I CCWWGG 4 cut(s) 7, 543, 624, 1514
EcoT38I GRGCYC 2 cut(s) 914, 1367
EgeI GGCGCC 1 cut(s) 586
EheI GGCGCC 1 cut(s) 586
ErhI CCWWGG 4 cut(s) 7, 543, 624, 1514
FaeI CATG 6 cut(s) 328, 412, 547, 977, 1256, 1316
FaqI GGGAC 2 cut(s) 491, 1137
FatI CATG 6 cut(s) 324, 408, 543, 973, 1252, 1312
FauI CCCGC 1 cut(s) 428
FbaI TGATCA 1 cut(s) 1237
Fnu4HI GCNGC 9 cut(s) 13, 30, 43, 95, 549, 552, 655, 1151, 1154
FokI GGATG 8 cut(s) 167, 512, 757, 1013, 1049, 1211, 1342, 1420
FriOI GRGCYC 2 cut(s) 914, 1367
Fsp4HI GCNGC 9 cut(s) 13, 30, 43, 95, 549, 552, 655, 1151, 1154
FspI TGCGCA 1 cut(s) 1412
GlaI GCGC 6 cut(s) 404, 464, 479, 586, 1098, 1412
GluI GCNGC 9 cut(s) 13, 30, 43, 95, 549, 552, 655, 1151, 1154
GsaI CCCAGC 3 cut(s) 20, 762, 1260
GsuI CTGGAG 4 cut(s) 92, 242, 494, 1304
HaeII RGCGCY 4 cut(s) 406, 481, 588, 1100
HapII CCGG 8 cut(s) 39, 98, 331, 483, 535, 1089, 1217, 1342
HgaI GACGC 3 cut(s) 265, 679, 1414
HhaI GCGC 6 cut(s) 405, 465, 480, 587, 1099, 1413
Hin1I GRCGYC 2 cut(s) 585, 671
Hin1II CATG 6 cut(s) 328, 412, 547, 977, 1256, 1316
Hin6I GCGC 6 cut(s) 403, 463, 478, 585, 1097, 1411
HinP1I GCGC 6 cut(s) 403, 463, 478, 585, 1097, 1411
HincII GTYRAC 2 cut(s) 312, 649
HindII GTYRAC 2 cut(s) 312, 649
HinfI GANTC 6 cut(s) 118, 443, 469, 637, 985, 1333
HpaII CCGG 8 cut(s) 39, 98, 331, 483, 535, 1089, 1217, 1342
HphI GGTGA 2 cut(s) 190, 418
Hpy166II GTNNAC 3 cut(s) 312, 319, 649
Hpy188I TCNGA 5 cut(s) 75, 491, 849, 1140, 1329
Hpy188III TCNNGA 7 cut(s) 109, 166, 325, 377, 473, 1028, 1102
Hpy8I GTNNAC 3 cut(s) 312, 319, 649
Hpy99I CGWCG 3 cut(s) 29, 443, 673
HpyAV CCTTC 2 cut(s) 84, 1257
HpyCH4III ACNGT 2 cut(s) 316, 1466
HpyCH4IV ACGT 3 cut(s) 24, 129, 504
HpyCH4V TGCA 9 cut(s) 229, 520, 605, 654, 753, 796, 1248, 1297, 1469
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 35, 1094, 1303
HpyF3I CTNAG 4 cut(s) 710, 783, 834, 846
HpySE526I ACGT 3 cut(s) 24, 129, 504
Hsp92I GRCGYC 2 cut(s) 585, 671
Hsp92II CATG 6 cut(s) 328, 412, 547, 977, 1256, 1316
HspAI GCGC 6 cut(s) 403, 463, 478, 585, 1097, 1411
KasI GGCGCC 1 cut(s) 584
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 1237
Kzo9I GATC 3 cut(s) 75, 775, 1237
LmnI GCTCC 7 cut(s) 255, 293, 475, 485, 726, 1030, 1370
Lsp1109I GCAGC 4 cut(s) 81, 666, 1140, 1162
LweI GCATC 2 cut(s) 507, 1398
MabI ACCWGGT 1 cut(s) 348
MaeII ACGT 3 cut(s) 24, 129, 504
MaeIII GTNAC 2 cut(s) 196, 1460
MalI GATC 3 cut(s) 77, 777, 1239
MboI GATC 3 cut(s) 75, 775, 1237
MboII GAAGA 6 cut(s) 295, 506, 572, 1060, 1158, 1300
MfeI CAATTG 1 cut(s) 889
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 2 cut(s) 914, 1367
MlsI TGGCCA 1 cut(s) 1082
MluCI AATT 4 cut(s) 134, 733, 850, 889
MluNI TGGCCA 1 cut(s) 1082
Mly113I GGCGCC 1 cut(s) 585
MlyI GAGTC 3 cut(s) 452, 979, 1327
Mox20I TGGCCA 1 cut(s) 1082
MroNI GCCGGC 1 cut(s) 38
MscI TGGCCA 1 cut(s) 1082
MseI TTAA 4 cut(s) 188, 203, 663, 1179
MslI CAYNNNNRTG 1 cut(s) 993
Msp20I TGGCCA 1 cut(s) 1082
MspA1I CMGCKG 1 cut(s) 1153
MspI CCGG 8 cut(s) 39, 98, 331, 483, 535, 1089, 1217, 1342
MspR9I CCNGG 6 cut(s) 331, 337, 350, 535, 590, 1217
MunI CAATTG 1 cut(s) 889
MvaI CCWGG 3 cut(s) 337, 350, 590
MvnI CGCG 2 cut(s) 993, 1425
MwoI GCNNNNNNNGC 4 cut(s) 26, 35, 1094, 1303
NaeI GCCGGC 1 cut(s) 40
NarI GGCGCC 1 cut(s) 585
NciI CCSGG 3 cut(s) 331, 535, 1217
NcoI CCATGG 1 cut(s) 543
NdeII GATC 3 cut(s) 75, 775, 1237
NgoMIV GCCGGC 1 cut(s) 38
NlaIII CATG 6 cut(s) 328, 412, 547, 977, 1256, 1316
NlaIV GGNNCC 5 cut(s) 77, 251, 393, 586, 1125
NmeAIII GCCGAG 2 cut(s) 592, 893
NmuCI GTSAC 1 cut(s) 196
NsbI TGCGCA 1 cut(s) 1412
NspI RCATGY 2 cut(s) 1256, 1316
PaeR7I CTCGAG 2 cut(s) 452, 1360
PagI TCATGA 1 cut(s) 324
PceI AGGCCT 1 cut(s) 212
PciI ACATGT 1 cut(s) 1312
PcsI WCGNNNNNNNCGW 3 cut(s) 312, 438, 683
PdiI GCCGGC 1 cut(s) 40
PfeI GAWTC 3 cut(s) 118, 469, 637
PfoI TCCNGGA 1 cut(s) 1215
PinAI ACCGGT 1 cut(s) 1341
PkrI GCNGC 9 cut(s) 14, 31, 44, 96, 550, 553, 656, 1152, 1155
PleI GAGTC 3 cut(s) 451, 979, 1327
PluTI GGCGCC 1 cut(s) 588
PpsI GAGTC 3 cut(s) 451, 979, 1327
Ppu21I YACGTR 1 cut(s) 130
PpuMI RGGWCCY 1 cut(s) 1124
PscI ACATGT 1 cut(s) 1312
PshAI GACNNNNGTC 1 cut(s) 503
Psp124BI GAGCTC 1 cut(s) 1367
Psp5II RGGWCCY 1 cut(s) 1124
Psp6I CCWGG 3 cut(s) 335, 348, 588
PspFI CCCAGC 3 cut(s) 16, 758, 1256
PspGI CCWGG 3 cut(s) 335, 348, 588
PspN4I GGNNCC 5 cut(s) 77, 251, 393, 586, 1125
PspPPI RGGWCCY 1 cut(s) 1124
PspXI VCTCGAGB 2 cut(s) 452, 1360
PstI CTGCAG 2 cut(s) 656, 1299
PsuI RGATCY 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 1153
RsaI GTAC 6 cut(s) 128, 263, 610, 832, 962, 1538
RsaNI GTAC 6 cut(s) 127, 262, 609, 831, 961, 1537
RseI CAYNNNNRTG 1 cut(s) 993
SacI GAGCTC 1 cut(s) 1367
SaqAI TTAA 4 cut(s) 188, 203, 663, 1179
SatI GCNGC 9 cut(s) 13, 30, 43, 95, 549, 552, 655, 1151, 1154
Sau3AI GATC 3 cut(s) 75, 775, 1237
ScaI AGTACT 1 cut(s) 832
SchI GAGTC 3 cut(s) 452, 979, 1327
ScrFI CCNGG 6 cut(s) 331, 337, 350, 535, 590, 1217
SduI GDGCHC 2 cut(s) 914, 1367
SexAI ACCWGGT 1 cut(s) 348
SfaNI GCATC 2 cut(s) 507, 1398
SfcI CTRYAG 2 cut(s) 652, 1295
SfoI GGCGCC 1 cut(s) 586
Sfr274I CTCGAG 2 cut(s) 452, 1360
SinI GGWCC 5 cut(s) 4, 223, 412, 1124, 1511
SlaI CTCGAG 2 cut(s) 452, 1360
SmiMI CAYNNNNRTG 1 cut(s) 993
SmlI CTYRAG 5 cut(s) 122, 386, 452, 1100, 1360
SmoI CTYRAG 5 cut(s) 122, 386, 452, 1100, 1360
Sse9I AATT 4 cut(s) 134, 733, 850, 889
SseBI AGGCCT 1 cut(s) 212
SspDI GGCGCC 1 cut(s) 584
SstI GAGCTC 1 cut(s) 1367
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 6 cut(s) 329, 335, 348, 533, 588, 1215
StyI CCWWGG 4 cut(s) 7, 543, 624, 1514
TaaI ACNGT 2 cut(s) 316, 1466
TaiI ACGT 3 cut(s) 27, 132, 507
TaqI TCGA 6 cut(s) 207, 354, 438, 453, 901, 1361
TasI AATT 4 cut(s) 134, 733, 850, 889
TatI WGTACW 3 cut(s) 261, 830, 960
TauI GCSGC 5 cut(s) 15, 32, 45, 551, 554
TfiI GAWTC 3 cut(s) 118, 469, 637
Tru1I TTAA 4 cut(s) 188, 203, 663, 1179
Tru9I TTAA 4 cut(s) 188, 203, 663, 1179
TscAI CASTG 2 cut(s) 760, 1471
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 4 cut(s) 94, 654, 1150, 1153
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 4 cut(s) 169, 397, 791, 962
TspRI CASTG 2 cut(s) 760, 1471
VpaK11BI GGWCC 5 cut(s) 4, 223, 412, 1124, 1511
XceI RCATGY 2 cut(s) 1256, 1316
XcmI CCANNNNNNNNNTGG 2 cut(s) 937, 1075
XhoI CTCGAG 2 cut(s) 452, 1360
ZrmI AGTACT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.