FvH4_2g40260

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012447
Physical Location & Seq
Forward (+)
28721201 .. 28723190
1990 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40260.t1

Sequence Viewer

Length: 1056 bp
ATGGCGCTCAGAAAAGCGCTAGCCAAGCGCTCATTTGAAGCCGTGAGAGTGACGACGTCTCTGACGCCATTGTCCGTCGTCGTTCCGGACCACCAAACCATCGTCCCACCTAATGCAGCGCAGGTGAACTTCCACAGGGAGTATCTCACCTCATCGGAACCCTCCGAGAAAGGCTTTTTCCGGCGATTCTTGCACCACTCCGCCACCAGAATCCCTGAGTTATTCTCCCTCCCCGTCGGAGAGAAGCTCCGGGAAAAGATCAATGGCTTGAACGTGAACATGCACAGGAAGGAAGATCGGCTCCGGCTCGAAGGGCTCAGCCCTCCGCCGGCCGCCGGGGACGAGTCGTTTTACGGAGTATCTGTGAACGATGTGAGGAAGATCATGAGGCTGTCTCAGGTGCAGAAGCTGAAGGCGAGGCTGAGAGAGGTTCCGGAGAGCTCCGTTTCGTATTCGGAGTTTCGCCGGATCTGCGTGGAAGGCTGCGAGAGCGTGGAGCAAGCGGCTGAGTTCGCGAAGCTTCTAGATGATTCCGGCAACGTCATCGTTCTCGGAAATGTTGTGTTTCTCCAGCCCGAGCAGGTTGCAAAATCAATAGAGAGCATAATCTCTCAAACCATGAGCCTGCCAAACGATCCAAGGAGAAGAGAGCTGGAGCAGCTAGAGAAGCAAAAGGTGATAATTGATCAAAAGGCCAGGGCCTTGGTGCGAGGTGAACTTTACGGTGGGCTGGGTTTTATGTTACTTCAGACACTTGGGGCCATAAGGCTAACCTTTTGGGAGCTAAGTTGGGATGTGATGGAGCCCATTTGCTTCTTTGTGACCTCCATCCACTTTGCTCTGGGCTACGGCTTCTTCCTCAGGACATACACTGAGCCCACCTTCCAAGGGTTCTTCCAACGCCGTTTCAAGACCAAGCAGCAAAAGCTCATGGAGGTCTACAACTTTGATGTTCAAAAGTACAACCAGCTCCGGAAAGTGTTTTATCCGAATTCCGGTAAACCAGTGTCGACGTTTGATCATGTAGAGACAACCCTTGTGGGTGATGTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04218 ko04621 map04020 map04218 map04621
Pfam Domains
Protein Families
MCU

Protein Analysis

352

Amino Acids

40.19

Weight (kDa)

9.22

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 166 - 324 7.3e-48 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42610
fragaria_vesca FvH4_2g40260
malus_domestica MD00G1134100.v1.1 MD08G1036100.v1.1 MD15G1032500.v1.1
prunus_persica Prupe.1G383500_v2.0.a1
pyrus_communis pycom08g02890 pycom15g02820
rosa_chinensis RchiOBHm_Chr6g0300501
rosa_laevigata RLG00000011332
rosa_multiflora Rmu_co8175312.1_g000001 Rmu_sc0001476.1_g000012
rosa_roxburghii Rroxscaffold_7G00167730
rosa_rugosa Rorug06G0296500
rosa_samantha Rh6AG409100 Rh6BG416400 Rh6CG424000 Rh6DG409600
rosa_wichuraiana Rw6G035800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 112
AasI GACNNNNNNGTC 1 cut(s) 70
AatII GACGTC 1 cut(s) 59
Acc36I ACCTGC 2 cut(s) 112, 571
AccI GTMKAC 2 cut(s) 939, 1010
AccII CGCG 1 cut(s) 515
AccIII TCCGGA 3 cut(s) 85, 433, 972
AciI CCGC 4 cut(s) 201, 326, 333, 503
AclWI GGATC 2 cut(s) 476, 629
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 991
AcuI CTGAAG 2 cut(s) 431, 731
AcyI GRCGYC 2 cut(s) 56, 65
AfaI GTAC 2 cut(s) 962, 1050
AfeI AGCGCT 2 cut(s) 18, 29
AfiI CCNNNNNNNGG 4 cut(s) 328, 335, 888, 995
AgsI TTSAA 4 cut(s) 38, 271, 910, 956
AjnI CCWGG 1 cut(s) 695
AluBI AGCT 9 cut(s) 247, 409, 441, 520, 652, 661, 784, 928, 970
AluI AGCT 9 cut(s) 247, 409, 441, 520, 652, 661, 784, 928, 970
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 3 cut(s) 63, 399, 1022
AlwI GGATC 2 cut(s) 476, 629
AlwNI CAGNNNCTG 1 cut(s) 409
Ama87I CYCGRG 1 cut(s) 575
Aor13HI TCCGGA 3 cut(s) 85, 433, 972
Aor51HI AGCGCT 2 cut(s) 18, 29
AoxI GGCC 4 cut(s) 330, 693, 699, 759
ApeKI GCWGC 4 cut(s) 116, 483, 658, 919
ApoI RAATTY 1 cut(s) 991
AspLEI GCGC 4 cut(s) 7, 19, 30, 121
AspS9I GGNCC 3 cut(s) 88, 699, 759
AsuC2I CCSGG 2 cut(s) 251, 337
AsuHPI GGTGA 5 cut(s) 136, 139, 688, 725, 1055
AsuNHI GCTAGC 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 575
AvaII GGWCC 1 cut(s) 88
AxyI CCTNAGG 1 cut(s) 860
BanII GRGCYC 4 cut(s) 318, 443, 807, 879
Bbv12I GWGCWC 1 cut(s) 443
BbvI GCAGC 4 cut(s) 128, 470, 670, 931
BccI CCATC 3 cut(s) 107, 793, 836
BceAI ACGGC 3 cut(s) 26, 865, 888
BcgI CGANNNNNNTGC 4 cut(s) 526, 560, 566, 600
BciT130I CCWGG 1 cut(s) 697
BclI TGATCA 2 cut(s) 685, 1018
BcnI CCSGG 2 cut(s) 251, 337
BcoDI GTCTC 3 cut(s) 63, 399, 1022
BfaI CTAG 3 cut(s) 20, 524, 662
BfoI RGCGCY 3 cut(s) 8, 20, 31
BfuAI ACCTGC 2 cut(s) 112, 571
BisI GCNGC 6 cut(s) 117, 333, 484, 504, 659, 920
BlpI GCTNAGC 1 cut(s) 317
BlsI GCNGC 6 cut(s) 118, 334, 485, 505, 660, 921
Bme1390I CCNGG 3 cut(s) 251, 337, 697
Bme18I GGWCC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 575
BmgT120I GGNCC 3 cut(s) 88, 699, 759
BmiI GGNNCC 5 cut(s) 159, 302, 432, 760, 804
BmrFI CCNGG 3 cut(s) 251, 337, 697
BmtI GCTAGC 1 cut(s) 23
BplI GAGNNNNNCTC 6 cut(s) 209, 241, 231, 263, 379, 411
BpmI CTGGAG 2 cut(s) 554, 674
Bpu1102I GCTNAGC 1 cut(s) 317
BpuMI CCSGG 2 cut(s) 251, 337
BsaHI GRCGYC 2 cut(s) 56, 65
BsaJI CCNNGG 5 cut(s) 336, 638, 696, 702, 886
BsaWI WCCGGW 4 cut(s) 85, 433, 972, 995
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 4 cut(s) 328, 335, 888, 995
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 1004
Bse21I CCTNAGG 1 cut(s) 860
BseAI TCCGGA 3 cut(s) 85, 433, 972
BseBI CCWGG 1 cut(s) 697
BseDI CCNNGG 5 cut(s) 336, 638, 696, 702, 886
BseGI GGATG 2 cut(s) 799, 828
BseLI CCNNNNNNNGG 4 cut(s) 328, 335, 888, 995
BseMII CTCAG 8 cut(s) 22, 207, 331, 410, 413, 498, 864, 874
BseNI ACTGG 1 cut(s) 1004
BseX3I CGGCCG 1 cut(s) 330
BseXI GCAGC 4 cut(s) 128, 470, 670, 931
BseYI CCCAGC 1 cut(s) 730
BsgI GTGCAG 1 cut(s) 422
Bsh1236I CGCG 1 cut(s) 515
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 4 cut(s) 332, 695, 701, 761
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 443
BsiHKCI CYCGRG 1 cut(s) 575
BslFI GGGAC 2 cut(s) 89, 353
BslI CCNNNNNNNGG 4 cut(s) 328, 335, 888, 995
BsmAI GTCTC 3 cut(s) 63, 399, 1022
BsmBI CGTCTC 1 cut(s) 63
BsmFI GGGAC 2 cut(s) 89, 353
BsnI GGCC 4 cut(s) 332, 695, 701, 761
BsoBI CYCGRG 1 cut(s) 575
Bsp1286I GDGCHC 4 cut(s) 318, 443, 807, 879
Bsp13I TCCGGA 3 cut(s) 85, 433, 972
Bsp1407I TGTACA 1 cut(s) 1048
Bsp143I GATC 7 cut(s) 258, 295, 381, 468, 634, 685, 1018
Bsp1720I GCTNAGC 1 cut(s) 317
Bsp68I TCGCGA 1 cut(s) 515
BspACI CCGC 4 cut(s) 201, 326, 333, 503
BspANI GGCC 4 cut(s) 332, 695, 701, 761
BspCNI CTCAG 8 cut(s) 21, 208, 330, 409, 414, 499, 865, 873
BspEI TCCGGA 3 cut(s) 85, 433, 972
BspFNI CGCG 1 cut(s) 515
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 5 cut(s) 159, 302, 432, 760, 804
BspMI ACCTGC 2 cut(s) 112, 571
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 2 cut(s) 476, 629
BsrFI RCCGGY 1 cut(s) 328
BsrGI TGTACA 1 cut(s) 1048
BsrI ACTGG 1 cut(s) 1004
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 5 cut(s) 336, 638, 696, 702, 886
BssMI GATC 7 cut(s) 258, 295, 381, 468, 634, 685, 1018
BssNI GRCGYC 2 cut(s) 56, 65
BssT1I CCWWGG 3 cut(s) 638, 702, 886
Bst2UI CCWGG 1 cut(s) 697
Bst4CI ACNGT 1 cut(s) 725
Bst6I CTCTTC 1 cut(s) 640
BstACI GRCGYC 2 cut(s) 56, 65
BstAUI TGTACA 1 cut(s) 1048
BstC8I GCNNGC 4 cut(s) 21, 330, 501, 626
BstDEI CTNAG 9 cut(s) 8, 216, 317, 396, 422, 507, 785, 860, 873
BstF5I GGATG 2 cut(s) 799, 828
BstFNI CGCG 1 cut(s) 515
BstH2I RGCGCY 3 cut(s) 8, 20, 31
BstHHI GCGC 4 cut(s) 7, 19, 30, 121
BstKTI GATC 7 cut(s) 261, 298, 384, 471, 637, 688, 1021
BstMAI GTCTC 3 cut(s) 63, 399, 1022
BstMBI GATC 7 cut(s) 258, 295, 381, 468, 634, 685, 1018
BstMCI CGRYCG 1 cut(s) 333
BstNI CCWGG 1 cut(s) 697
BstNSI RCATGY 1 cut(s) 283
BstSCI CCNGG 3 cut(s) 249, 335, 695
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 4 cut(s) 128, 470, 670, 931
BstX2I RGATCY 1 cut(s) 468
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 1 cut(s) 468
BstZI CGGCCG 1 cut(s) 330
Bsu36I CCTNAGG 1 cut(s) 860
BsuRI GGCC 4 cut(s) 332, 695, 701, 761
BtsCI GGATG 2 cut(s) 799, 828
BtsIMutI CAGTG 2 cut(s) 870, 1011
BtuMI TCGCGA 1 cut(s) 515
BveI ACCTGC 2 cut(s) 112, 571
Cac8I GCNNGC 4 cut(s) 21, 330, 501, 626
CaiI CAGNNNCTG 1 cut(s) 409
CciI TCATGA 1 cut(s) 384
CfoI GCGC 4 cut(s) 7, 19, 30, 121
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 88, 699, 759
CseI GACGC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 961, 1049
CspCI CAANNNNNGTGG 2 cut(s) 1020, 1055
CviAII CATG 5 cut(s) 280, 385, 619, 931, 1022
CviQI GTAC 2 cut(s) 961, 1049
DdeI CTNAG 9 cut(s) 8, 216, 317, 396, 422, 507, 785, 860, 873
DpnI GATC 7 cut(s) 260, 297, 383, 470, 636, 687, 1020
DpnII GATC 7 cut(s) 258, 295, 381, 468, 634, 685, 1018
DrdI GACNNNNNNGTC 1 cut(s) 70
DseDI GACNNNNNNGTC 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 330
EagI CGGCCG 1 cut(s) 330
Eam1104I CTCTTC 1 cut(s) 640
EarI CTCTTC 1 cut(s) 640
EciI GGCGGA 2 cut(s) 190, 315
Ecl136II GAGCTC 1 cut(s) 441
EclXI CGGCCG 1 cut(s) 330
Eco130I CCWWGG 3 cut(s) 638, 702, 886
Eco24I GRGCYC 4 cut(s) 318, 443, 807, 879
Eco47I GGWCC 1 cut(s) 88
Eco47III AGCGCT 2 cut(s) 18, 29
Eco52I CGGCCG 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 441
Eco57I CTGAAG 2 cut(s) 431, 731
Eco81I CCTNAGG 1 cut(s) 860
Eco88I CYCGRG 1 cut(s) 575
EcoICRI GAGCTC 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 699
EcoRI GAATTC 1 cut(s) 991
EcoRII CCWGG 1 cut(s) 695
EcoT14I CCWWGG 3 cut(s) 638, 702, 886
EcoT38I GRGCYC 4 cut(s) 318, 443, 807, 879
ErhI CCWWGG 3 cut(s) 638, 702, 886
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 5 cut(s) 283, 388, 622, 934, 1025
FaiI YATR 9 cut(s) 281, 386, 605, 620, 740, 764, 868, 932, 1023
FaqI GGGAC 2 cut(s) 89, 353
FatI CATG 5 cut(s) 279, 384, 618, 930, 1021
FbaI TGATCA 2 cut(s) 685, 1018
FblI GTMKAC 2 cut(s) 939, 1010
Fnu4HI GCNGC 6 cut(s) 117, 333, 484, 504, 659, 920
FokI GGATG 2 cut(s) 806, 815
FriOI GRGCYC 4 cut(s) 318, 443, 807, 879
Fsp4HI GCNGC 6 cut(s) 117, 333, 484, 504, 659, 920
FspBI CTAG 3 cut(s) 20, 524, 662
GlaI GCGC 4 cut(s) 6, 18, 29, 120
GluI GCNGC 6 cut(s) 117, 333, 484, 504, 659, 920
GsaI CCCAGC 1 cut(s) 734
GsuI CTGGAG 2 cut(s) 554, 674
HaeII RGCGCY 3 cut(s) 8, 20, 31
HaeIII GGCC 4 cut(s) 332, 695, 701, 761
HgaI GACGC 1 cut(s) 73
HhaI GCGC 4 cut(s) 7, 19, 30, 121
Hin1I GRCGYC 2 cut(s) 56, 65
Hin1II CATG 5 cut(s) 283, 388, 622, 934, 1025
Hin6I GCGC 4 cut(s) 5, 17, 28, 119
HinP1I GCGC 4 cut(s) 5, 17, 28, 119
HincII GTYRAC 1 cut(s) 1011
HindII GTYRAC 1 cut(s) 1011
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 4 cut(s) 186, 210, 344, 530
HphI GGTGA 5 cut(s) 136, 139, 688, 725, 1055
Hpy166II GTNNAC 7 cut(s) 127, 277, 367, 716, 940, 1001, 1011
Hpy188I TCNGA 9 cut(s) 11, 63, 157, 166, 239, 457, 554, 750, 990
Hpy188III TCNNGA 8 cut(s) 86, 385, 434, 514, 524, 862, 910, 973
Hpy8I GTNNAC 7 cut(s) 127, 277, 367, 716, 940, 1001, 1011
Hpy99I CGWCG 5 cut(s) 58, 80, 83, 239, 1015
HpyAV CCTTC 5 cut(s) 283, 305, 406, 473, 892
HpyCH4III ACNGT 1 cut(s) 725
HpyCH4IV ACGT 4 cut(s) 56, 273, 540, 1013
HpyCH4V TGCA 5 cut(s) 116, 193, 283, 403, 587
HpyF3I CTNAG 9 cut(s) 8, 216, 317, 396, 422, 507, 785, 860, 873
HpySE526I ACGT 4 cut(s) 56, 273, 540, 1013
Hsp92I GRCGYC 2 cut(s) 56, 65
Hsp92II CATG 5 cut(s) 283, 388, 622, 934, 1025
HspAI GCGC 4 cut(s) 5, 17, 28, 119
Kpn2I TCCGGA 3 cut(s) 85, 433, 972
KroI GCCGGC 1 cut(s) 328
KroNI GCCGGC 1 cut(s) 330
Ksp22I TGATCA 2 cut(s) 685, 1018
Kzo9I GATC 7 cut(s) 258, 295, 381, 468, 634, 685, 1018
LmnI GCTCC 8 cut(s) 252, 306, 446, 496, 655, 781, 802, 975
Lsp1109I GCAGC 4 cut(s) 128, 470, 670, 931
MaeI CTAG 3 cut(s) 20, 524, 662
MaeII ACGT 4 cut(s) 56, 273, 540, 1013
MaeIII GTNAC 3 cut(s) 49, 741, 820
MalI GATC 7 cut(s) 260, 297, 383, 470, 636, 687, 1020
MboI GATC 7 cut(s) 258, 295, 381, 468, 634, 685, 1018
MboII GAAGA 5 cut(s) 305, 391, 657, 847, 886
MflI RGATCY 1 cut(s) 468
MhlI GDGCHC 4 cut(s) 318, 443, 807, 879
MluCI AATT 2 cut(s) 681, 991
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 2 cut(s) 217, 922
MroI TCCGGA 3 cut(s) 85, 433, 972
MroNI GCCGGC 1 cut(s) 328
MspR9I CCNGG 3 cut(s) 251, 337, 697
MvaI CCWGG 1 cut(s) 697
MvnI CGCG 1 cut(s) 515
NaeI GCCGGC 1 cut(s) 330
NciI CCSGG 2 cut(s) 251, 337
NdeII GATC 7 cut(s) 258, 295, 381, 468, 634, 685, 1018
NgoMIV GCCGGC 1 cut(s) 328
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 5 cut(s) 283, 388, 622, 934, 1025
NlaIV GGNNCC 5 cut(s) 159, 302, 432, 760, 804
NmuCI GTSAC 2 cut(s) 49, 820
NruI TCGCGA 1 cut(s) 515
NspI RCATGY 1 cut(s) 283
PagI TCATGA 1 cut(s) 384
PaqCI CACCTGC 1 cut(s) 112
PdiI GCCGGC 1 cut(s) 330
PfeI GAWTC 3 cut(s) 186, 210, 530
PflFI GACNNNGTC 1 cut(s) 55
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 6 cut(s) 118, 334, 485, 505, 660, 921
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
Psp124BI GAGCTC 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 695
PspFI CCCAGC 1 cut(s) 730
PspGI CCWGG 1 cut(s) 695
PspN4I GGNNCC 5 cut(s) 159, 302, 432, 760, 804
PspPI GGNCC 3 cut(s) 88, 699, 759
PstNI CAGNNNCTG 1 cut(s) 409
PsuI RGATCY 1 cut(s) 468
PsyI GACNNNGTC 1 cut(s) 55
RruI TCGCGA 1 cut(s) 515
RsaI GTAC 2 cut(s) 962, 1050
RsaNI GTAC 2 cut(s) 961, 1049
SacI GAGCTC 1 cut(s) 443
SalI GTCGAC 1 cut(s) 1009
SatI GCNGC 6 cut(s) 117, 333, 484, 504, 659, 920
Sau3AI GATC 7 cut(s) 258, 295, 381, 468, 634, 685, 1018
Sau96I GGNCC 3 cut(s) 88, 699, 759
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 3 cut(s) 251, 337, 697
SduI GDGCHC 4 cut(s) 318, 443, 807, 879
SinI GGWCC 1 cut(s) 88
Sse9I AATT 2 cut(s) 681, 991
SsiI CCGC 4 cut(s) 201, 326, 333, 503
SspMI CTAG 3 cut(s) 20, 524, 662
SstI GAGCTC 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 249, 335, 695
StyI CCWWGG 3 cut(s) 638, 702, 886
TaaI ACNGT 1 cut(s) 725
TaiI ACGT 4 cut(s) 59, 276, 543, 1016
TaqI TCGA 2 cut(s) 309, 1010
TasI AATT 2 cut(s) 681, 991
TatI WGTACW 2 cut(s) 960, 1048
TauI GCSGC 2 cut(s) 335, 506
TfiI GAWTC 3 cut(s) 186, 210, 530
TscAI CASTG 2 cut(s) 877, 1011
TseFI GTSAC 2 cut(s) 49, 820
TseI GCWGC 4 cut(s) 116, 483, 658, 919
Tsp45I GTSAC 2 cut(s) 49, 820
TspGWI ACGGA 3 cut(s) 64, 369, 433
TspRI CASTG 2 cut(s) 877, 1011
Tth111I GACNNNGTC 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 88
XapI RAATTY 1 cut(s) 991
XbaI TCTAGA 1 cut(s) 523
XceI RCATGY 1 cut(s) 283
XmiI GTMKAC 2 cut(s) 939, 1010
XspI CTAG 3 cut(s) 20, 524, 662
ZraI GACGTC 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.