MD00G1134100.v1.1

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012447
Physical Location & Seq
Reverse (-)
29034374 .. 29034982
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1134100.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGCGCTCCGTCAAGCGCTTGCTAAGCGCGCATTTGGCGCCACCAGAGTGGCCCTAGCGCCACCGTCGTCGGTTATGGCAGTTCACCAAAACATCGTGCCTCCGAATGCCGCCCAGGTGAATTTCCACAGGGAGTATCTGACCTCGATGGACACCCCCCGGAAAGGATTTTTCCGCCGATTCCTCCACCGCCAGTCGGCATCGAAGCTCCCGGAATTCCTGACCGTTCCGGTTGGGGAGAAGCTCCGGGAAAAGCTGAGAGGCATCAACATTAGCGGTGATCAGCTCCGTCTCGGTGGTCTCAGCCCTCCGCCTCCAGATCGGGACCCCACCGCCAAGCTTCCGTTAGGAATCTCGGTCCCCAACGCGAAGAAGATTCTGAGGCTTTCTCAGGTGGAGAAGCTGAAGGCGAAGCTGAGGGAGATTCCGGAAACTTCCATTTCGCGATCGGAGTTCGTTCGGATCTGCAGCGAAGGATGCGAGAGCGAAGAGCAAGGTGCTGAGTTCGCGAAGATGATGGACGAGTCCGTAAACGTTATCGTTTTGGGAAACGTAGTGTTTCTCCGGCCGGAGCAGGTAACTTCTTCTTCTTTCCCGCTTTTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04218 ko04621 map04020 map04218 map04621
Pfam Domains
Protein Families
MCU

Protein Analysis

203

Amino Acids

22.41

Weight (kDa)

9.94

Isoelectric Point (pI)

56.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42610
fragaria_vesca FvH4_2g40260
malus_domestica MD00G1134100.v1.1 MD08G1036100.v1.1 MD15G1032500.v1.1
prunus_persica Prupe.1G383500_v2.0.a1
pyrus_communis pycom08g02890 pycom15g02820
rosa_chinensis RchiOBHm_Chr6g0300501
rosa_laevigata RLG00000011332
rosa_multiflora Rmu_co8175312.1_g000001 Rmu_sc0001476.1_g000012
rosa_roxburghii Rroxscaffold_7G00167730
rosa_rugosa Rorug06G0296500
rosa_samantha Rh6AG409100 Rh6BG416400 Rh6CG424000 Rh6DG409600
rosa_wichuraiana Rw6G035800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 38
AccII CGCG 4 cut(s) 30, 368, 445, 509
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 7 cut(s) 111, 175, 190, 276, 311, 333, 596
AclI AACGTT 1 cut(s) 534
AclWI GGATC 1 cut(s) 470
AcoI YGGCCR 1 cut(s) 566
AcsI RAATTY 2 cut(s) 121, 215
AcuI CTGAAG 1 cut(s) 425
AcyI GRCGYC 1 cut(s) 39
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 2 cut(s) 164, 196
AjnI CCWGG 1 cut(s) 114
AleI CACNNNNGTG 1 cut(s) 47
AluBI AGCT 7 cut(s) 208, 244, 256, 286, 340, 403, 415
AluI AGCT 7 cut(s) 208, 244, 256, 286, 340, 403, 415
Alw26I GTCTC 2 cut(s) 296, 305
AlwI GGATC 1 cut(s) 470
Aor13HI TCCGGA 1 cut(s) 427
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 2 cut(s) 51, 566
ApeKI GCWGC 1 cut(s) 468
ApoI RAATTY 2 cut(s) 121, 215
AspLEI GCGC 6 cut(s) 7, 19, 30, 32, 41, 61
AspS9I GGNCC 3 cut(s) 52, 325, 358
AsuC2I CCSGG 3 cut(s) 160, 212, 248
AsuHPI GGTGA 3 cut(s) 77, 130, 290
AvaII GGWCC 2 cut(s) 325, 358
BanI GGYRCC 1 cut(s) 38
BbvCI CCTCAGC 1 cut(s) 416
BbvI GCAGC 1 cut(s) 480
BccI CCATC 2 cut(s) 142, 511
BciT130I CCWGG 1 cut(s) 116
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 3 cut(s) 160, 212, 248
BcoDI GTCTC 2 cut(s) 296, 305
BfaI CTAG 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 466
BfoI RGCGCY 4 cut(s) 8, 20, 42, 62
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 2 cut(s) 111, 469
BlpI GCTNAGC 1 cut(s) 24
BlsI GCNGC 2 cut(s) 112, 470
Bme1390I CCNGG 4 cut(s) 116, 160, 212, 248
Bme18I GGWCC 2 cut(s) 325, 358
BmgT120I GGNCC 3 cut(s) 52, 325, 358
BmiI GGNNCC 4 cut(s) 40, 326, 327, 360
BmrFI CCNGG 4 cut(s) 116, 160, 212, 248
BmsI GCATC 3 cut(s) 209, 273, 467
BplI GAGNNNNNCTC 2 cut(s) 373, 405
BpmI CTGGAG 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 416
Bpu1102I GCTNAGC 1 cut(s) 24
BpuMI CCSGG 3 cut(s) 160, 212, 248
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 114, 158
BsaWI WCCGGW 2 cut(s) 229, 427
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 196
Bse1I ACTGG 1 cut(s) 193
BseAI TCCGGA 1 cut(s) 427
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 2 cut(s) 114, 158
BseGI GGATG 1 cut(s) 482
BseLI CCNNNNNNNGG 2 cut(s) 164, 196
BseMII CTCAG 6 cut(s) 248, 316, 371, 404, 407, 492
BseNI ACTGG 1 cut(s) 193
BsePI GCGCGC 1 cut(s) 28
BseX3I CGGCCG 1 cut(s) 566
BseXI GCAGC 1 cut(s) 480
Bsh1236I CGCG 4 cut(s) 30, 368, 445, 509
Bsh1285I CGRYCG 2 cut(s) 449, 569
BshFI GGCC 2 cut(s) 53, 568
BshNI GGYRCC 1 cut(s) 38
BsiEI CGRYCG 2 cut(s) 449, 569
BsiSI CCGG 7 cut(s) 160, 212, 230, 247, 428, 565, 569
BslFI GGGAC 2 cut(s) 338, 344
BslI CCNNNNNNNGG 2 cut(s) 164, 196
BsmAI GTCTC 2 cut(s) 296, 305
BsmBI CGTCTC 1 cut(s) 296
BsmFI GGGAC 2 cut(s) 338, 344
BsmI GAATGC 1 cut(s) 112
BsnI GGCC 2 cut(s) 53, 568
Bso31I GGTCTC 1 cut(s) 305
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 4 cut(s) 280, 319, 446, 462
Bsp1720I GCTNAGC 1 cut(s) 24
Bsp68I TCGCGA 2 cut(s) 445, 509
BspACI CCGC 7 cut(s) 111, 175, 190, 276, 311, 333, 596
BspANI GGCC 2 cut(s) 53, 568
BspCNI CTCAG 6 cut(s) 249, 315, 372, 403, 408, 493
BspEI TCCGGA 1 cut(s) 427
BspFNI CGCG 4 cut(s) 30, 368, 445, 509
BspLI GGNNCC 4 cut(s) 40, 326, 327, 360
BspMAI CTGCAG 1 cut(s) 470
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 1 cut(s) 470
BspQI GCTCTTC 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 305
BsrI ACTGG 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 114, 158
BssHII GCGCGC 1 cut(s) 28
BssMI GATC 4 cut(s) 280, 319, 446, 462
BssNI GRCGYC 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 66, 226
Bst6I CTCTTC 1 cut(s) 483
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 21, 30
BstDEI CTNAG 7 cut(s) 24, 257, 302, 380, 390, 416, 501
BstF5I GGATG 1 cut(s) 482
BstFNI CGCG 4 cut(s) 30, 368, 445, 509
BstH2I RGCGCY 4 cut(s) 8, 20, 42, 62
BstHHI GCGC 6 cut(s) 7, 19, 30, 32, 41, 61
BstKTI GATC 4 cut(s) 283, 322, 449, 465
BstMAI GTCTC 2 cut(s) 296, 305
BstMBI GATC 4 cut(s) 280, 319, 446, 462
BstMCI CGRYCG 2 cut(s) 449, 569
BstMWI GCNNNNNNNGC 6 cut(s) 25, 29, 36, 38, 477, 506
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 4 cut(s) 114, 158, 210, 246
BstSFI CTRYAG 1 cut(s) 466
BstUI CGCG 4 cut(s) 30, 368, 445, 509
BstV1I GCAGC 1 cut(s) 480
BstX2I RGATCY 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 1 cut(s) 462
BstZI CGGCCG 1 cut(s) 566
BsuRI GGCC 2 cut(s) 53, 568
BtsCI GGATG 1 cut(s) 482
BtuMI TCGCGA 2 cut(s) 445, 509
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 2 cut(s) 21, 30
CfoI GCGC 6 cut(s) 7, 19, 30, 32, 41, 61
Cfr13I GGNCC 3 cut(s) 52, 325, 358
DdeI CTNAG 7 cut(s) 24, 257, 302, 380, 390, 416, 501
DinI GGCGCC 1 cut(s) 40
DpnI GATC 4 cut(s) 282, 321, 448, 464
DpnII GATC 4 cut(s) 280, 319, 446, 462
EaeI YGGCCR 1 cut(s) 566
EagI CGGCCG 1 cut(s) 566
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
EciI GGCGGA 2 cut(s) 164, 300
EclXI CGGCCG 1 cut(s) 566
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 2 cut(s) 325, 358
Eco47III AGCGCT 1 cut(s) 18
Eco52I CGGCCG 1 cut(s) 566
Eco57I CTGAAG 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 114
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
Esp3I CGTCTC 1 cut(s) 296
FaiI YATR 2 cut(s) 77, 605
FaqI GGGAC 2 cut(s) 338, 344
FauI CCCGC 1 cut(s) 603
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 2 cut(s) 111, 469
FokI GGATG 1 cut(s) 489
Fsp4HI GCNGC 2 cut(s) 111, 469
FspBI CTAG 1 cut(s) 56
GlaI GCGC 6 cut(s) 6, 18, 29, 31, 40, 60
GluI GCNGC 2 cut(s) 111, 469
GsuI CTGGAG 1 cut(s) 300
HaeII RGCGCY 4 cut(s) 8, 20, 42, 62
HaeIII GGCC 2 cut(s) 53, 568
HapII CCGG 7 cut(s) 160, 212, 230, 247, 428, 565, 569
HhaI GCGC 6 cut(s) 7, 19, 30, 32, 41, 61
Hin1I GRCGYC 1 cut(s) 39
Hin6I GCGC 6 cut(s) 5, 17, 28, 30, 39, 59
HinP1I GCGC 6 cut(s) 5, 17, 28, 30, 39, 59
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 5 cut(s) 180, 351, 376, 424, 524
HpaII CCGG 7 cut(s) 160, 212, 230, 247, 428, 565, 569
HphI GGTGA 3 cut(s) 77, 130, 290
Hpy166II GTNNAC 2 cut(s) 85, 532
Hpy188I TCNGA 5 cut(s) 105, 141, 381, 451, 462
Hpy188III TCNNGA 6 cut(s) 220, 317, 323, 428, 444, 508
Hpy8I GTNNAC 2 cut(s) 85, 532
Hpy99I CGWCG 2 cut(s) 70, 73
HpyAV CCTTC 2 cut(s) 400, 467
HpyCH4III ACNGT 2 cut(s) 66, 226
HpyCH4IV ACGT 2 cut(s) 534, 552
HpyCH4V TGCA 1 cut(s) 468
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 29, 36, 38, 477, 506
HpyF3I CTNAG 7 cut(s) 24, 257, 302, 380, 390, 416, 501
HpySE526I ACGT 2 cut(s) 534, 552
Hsp92I GRCGYC 1 cut(s) 39
HspAI GCGC 6 cut(s) 5, 17, 28, 30, 39, 59
KasI GGCGCC 1 cut(s) 38
KflI GGGWCCC 1 cut(s) 325
Kpn2I TCCGGA 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 4 cut(s) 280, 319, 446, 462
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 5 cut(s) 12, 213, 249, 291, 571
Lsp1109I GCAGC 1 cut(s) 480
LweI GCATC 3 cut(s) 209, 273, 467
MaeI CTAG 1 cut(s) 56
MaeII ACGT 2 cut(s) 534, 552
MaeIII GTNAC 1 cut(s) 577
MalI GATC 4 cut(s) 282, 321, 448, 464
MboI GATC 4 cut(s) 280, 319, 446, 462
MboII GAAGA 6 cut(s) 382, 385, 500, 523, 576, 579
MflI RGATCY 1 cut(s) 462
MluCI AATT 2 cut(s) 121, 215
Mly113I GGCGCC 1 cut(s) 39
MlyI GAGTC 1 cut(s) 533
MnlI CCTC 8 cut(s) 111, 154, 194, 254, 318, 324, 375, 411
MroI TCCGGA 1 cut(s) 427
MslI CAYNNNNRTG 1 cut(s) 47
MspI CCGG 7 cut(s) 160, 212, 230, 247, 428, 565, 569
MspR9I CCNGG 4 cut(s) 116, 160, 212, 248
Mva1269I GAATGC 1 cut(s) 112
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 4 cut(s) 30, 368, 445, 509
MwoI GCNNNNNNNGC 6 cut(s) 25, 29, 36, 38, 477, 506
NarI GGCGCC 1 cut(s) 39
NciI CCSGG 3 cut(s) 160, 212, 248
NdeII GATC 4 cut(s) 280, 319, 446, 462
NlaIV GGNNCC 4 cut(s) 40, 326, 327, 360
NruI TCGCGA 2 cut(s) 445, 509
OliI CACNNNNGTG 1 cut(s) 47
PauI GCGCGC 1 cut(s) 28
PciSI GCTCTTC 1 cut(s) 483
PctI GAATGC 1 cut(s) 112
PfeI GAWTC 4 cut(s) 180, 351, 376, 424
PfoI TCCNGGA 2 cut(s) 210, 246
PkrI GCNGC 2 cut(s) 112, 470
Ple19I CGATCG 1 cut(s) 449
PleI GAGTC 1 cut(s) 532
PluTI GGCGCC 1 cut(s) 42
PpsI GAGTC 1 cut(s) 532
PpuMI RGGWCCY 1 cut(s) 325
Psp1406I AACGTT 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 4 cut(s) 40, 326, 327, 360
PspPI GGNCC 3 cut(s) 52, 325, 358
PspPPI RGGWCCY 1 cut(s) 325
PstI CTGCAG 1 cut(s) 470
PsuI RGATCY 1 cut(s) 462
PteI GCGCGC 1 cut(s) 28
PvuI CGATCG 1 cut(s) 449
RruI TCGCGA 2 cut(s) 445, 509
RseI CAYNNNNRTG 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 483
SatI GCNGC 2 cut(s) 111, 469
Sau3AI GATC 4 cut(s) 280, 319, 446, 462
Sau96I GGNCC 3 cut(s) 52, 325, 358
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 4 cut(s) 116, 160, 212, 248
SfaNI GCATC 3 cut(s) 209, 273, 467
SfcI CTRYAG 1 cut(s) 466
SfoI GGCGCC 1 cut(s) 40
SinI GGWCC 2 cut(s) 325, 358
SmiMI CAYNNNNRTG 1 cut(s) 47
Sse9I AATT 2 cut(s) 121, 215
SsiI CCGC 7 cut(s) 111, 175, 190, 276, 311, 333, 596
SspDI GGCGCC 1 cut(s) 38
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 4 cut(s) 114, 158, 210, 246
TaaI ACNGT 2 cut(s) 66, 226
TaiI ACGT 2 cut(s) 537, 555
TaqI TCGA 2 cut(s) 146, 203
TaqII GACCGA 1 cut(s) 346
TasI AATT 2 cut(s) 121, 215
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 4 cut(s) 180, 351, 376, 424
TseI GCWGC 1 cut(s) 468
TspGWI ACGGA 3 cut(s) 278, 333, 517
VpaK11BI GGWCC 2 cut(s) 325, 358
XapI RAATTY 2 cut(s) 121, 215
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.