Rroxscaffold_7G00167730

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012447
Physical Location & Seq
Forward (+)
8779552 .. 8781672
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167730.1

Sequence Viewer

Length: 1047 bp
ATGGCGCTCAGAAAAGCGCTAGCCAAGCGTTCACTCGACGCCGTCAGGGTGACGTCACTGACACCATTCTCCGTGGTCGTTCTAGGGCACCAAACCATTGTTCCACCTAATGCAGCCCAGGCAAACTTCCATAGGGAGTATCTCACCTCATCGGAGCCCGCCGAGGGCTTTTTCCGCCGATTCTTCCACCACTCCGCCACCAGGATCCCGGAGTTCCTCTCCCTGCCTGTCGGAGAGAAGCTCCGTGAAAAGATCAAAGGTGTAAACAGGAACGGAGATCGGCTCCAGCTCGCCGGACTCAGCCCTCCTCCGCCGGATCCGGCCACCGCTGACGAGTTGTTTTACGGAATCTCCGTCAGCGATGTGCGGAAGATTATGAGGCTGTCGCAGGTGGAGAAGCTGAAGGCGAAGCTGAGAGAGGTTCCGGAGAGCTCAGTTTCGTATTCGGAGTTCCGCCGGATCTGCGTGGAAGGTTGCGAGAGCGAAGAGCAAGCAGCTGAGTTTGCGAAGCTTCTCGATGAGTCCGGCAACGTCATCGTTCTCGGAAATGTTGTGTTTCTCCGGCCCGAGCAGGTTGCAAAATCAATGGAGAGCATAATTTCTGAAACCATGGGCCTGCCAAACGATCCAAGGAGGAGAGAACTGGACCAGCTAGAGAAGCAAAAGAAGGTAATAGACCAAAGGGCCAGGGCCTTAGTGCAAGGTGAACTCTACGGTGGGCTGGGTTTTATACTACTCCAGACACTTGGTGCCATAAGGCTCACCTTTTGGGAGCTAAGTTGGGATGTGATGGAGCCCATTTGCTTCTTTGTGACCTCCATCTACTTTGCTATGGGCTACGGCTTTTTCCTCAGGACATCCACAGAGCCCACTTTCCAAGGGTTCTTCCAACGCCGTTTTAAGACCAAGCAGCAACGGCTCATGGAGATCTATAACTTTGATGTTCGAAAGTACAACCAGCTCCGGAAAGTGTTCTATCCGAATTCCGATCAACTGGCGTCGTTAGATTATGTAGAAACAACCCTTGTTGGTGCTTTACATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04218 ko04621 map04020 map04218 map04621
Pfam Domains
Protein Families
MCU

Protein Analysis

348

Amino Acids

39.73

Weight (kDa)

9.12

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 162 - 321 5.9e-48 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42610
fragaria_vesca FvH4_2g40260
malus_domestica MD00G1134100.v1.1 MD08G1036100.v1.1 MD15G1032500.v1.1
prunus_persica Prupe.1G383500_v2.0.a1
pyrus_communis pycom08g02890 pycom15g02820
rosa_chinensis RchiOBHm_Chr6g0300501
rosa_laevigata RLG00000011332
rosa_multiflora Rmu_co8175312.1_g000001 Rmu_sc0001476.1_g000012
rosa_roxburghii Rroxscaffold_7G00167730
rosa_rugosa Rorug06G0296500
rosa_samantha Rh6AG409100 Rh6BG416400 Rh6CG424000 Rh6DG409600
rosa_wichuraiana Rw6G035800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 379
AatII GACGTC 1 cut(s) 56
Acc36I ACCTGC 2 cut(s) 379, 562
AccB1I GGYRCC 2 cut(s) 87, 749
AccIII TCCGGA 2 cut(s) 424, 963
AciI CCGC 7 cut(s) 159, 175, 195, 311, 327, 367, 454
AclWI GGATC 6 cut(s) 199, 212, 311, 324, 467, 620
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 982
AcuI CTGAAG 1 cut(s) 422
AcyI GRCGYC 3 cut(s) 39, 53, 998
AdeI CACNNNGTG 1 cut(s) 749
AfaI GTAC 1 cut(s) 953
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 2 cut(s) 164, 201
AjnI CCWGG 3 cut(s) 117, 200, 686
AjuI GAANNNNNNNTTGG 2 cut(s) 84, 116
Alw21I GWGCWC 1 cut(s) 434
AlwI GGATC 6 cut(s) 199, 212, 311, 324, 467, 620
Ama87I CYCGRG 1 cut(s) 566
Aor13HI TCCGGA 2 cut(s) 424, 963
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 5 cut(s) 321, 563, 613, 684, 690
ApeKI GCWGC 3 cut(s) 113, 494, 910
ApoI RAATTY 1 cut(s) 982
Asp700I GAANNNNTTC 1 cut(s) 971
AspLEI GCGC 2 cut(s) 7, 19
AspS9I GGNCC 5 cut(s) 564, 613, 646, 684, 690
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 4 cut(s) 61, 136, 716, 754
AsuII TTCGAA 1 cut(s) 946
AsuNHI GCTAGC 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 566
AvaII GGWCC 1 cut(s) 646
AxyI CCTNAGG 1 cut(s) 851
BaeGI GKGCMC 1 cut(s) 90
BamHI GGATCC 2 cut(s) 204, 316
BanI GGYRCC 2 cut(s) 87, 749
BanII GRGCYC 4 cut(s) 159, 434, 798, 870
Bbv12I GWGCWC 1 cut(s) 434
BbvI GCAGC 3 cut(s) 125, 506, 922
BccI CCATC 2 cut(s) 784, 827
BceAI ACGGC 4 cut(s) 26, 856, 879, 932
BcgI CGANNNNNNTGC 4 cut(s) 517, 551, 557, 591
BciT130I CCWGG 3 cut(s) 119, 202, 688
BcnI CCSGG 1 cut(s) 209
BfaI CTAG 3 cut(s) 20, 83, 653
BfoI RGCGCY 2 cut(s) 8, 20
BfuAI ACCTGC 2 cut(s) 379, 562
BglII AGATCT 1 cut(s) 927
BisI GCNGC 3 cut(s) 114, 495, 911
BlsI GCNGC 3 cut(s) 115, 496, 912
Bme1390I CCNGG 4 cut(s) 119, 202, 209, 688
Bme18I GGWCC 1 cut(s) 646
BmeT110I CYCGRG 1 cut(s) 566
BmgT120I GGNCC 5 cut(s) 564, 613, 646, 684, 690
BmiI GGNNCC 8 cut(s) 89, 156, 206, 284, 318, 423, 751, 795
BmrFI CCNGG 4 cut(s) 119, 202, 209, 688
BmtI GCTAGC 1 cut(s) 23
BplI GAGNNNNNCTC 6 cut(s) 203, 235, 225, 257, 267, 299
BpmI CTGGAG 2 cut(s) 269, 722
Bpu14I TTCGAA 1 cut(s) 946
BpuMI CCSGG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 1042
BsaHI GRCGYC 3 cut(s) 39, 53, 998
BsaJI CCNNGG 7 cut(s) 72, 117, 162, 609, 629, 687, 877
BsaWI WCCGGW 2 cut(s) 424, 963
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 201
Bse1I ACTGG 2 cut(s) 648, 999
Bse21I CCTNAGG 1 cut(s) 851
BseAI TCCGGA 2 cut(s) 424, 963
BseBI CCWGG 3 cut(s) 119, 202, 688
BseCI ATCGAT 1 cut(s) 1042
BseDI CCNNGG 7 cut(s) 72, 117, 162, 609, 629, 687, 877
BseGI GGATG 2 cut(s) 790, 857
BseLI CCNNNNNNNGG 2 cut(s) 164, 201
BseMII CTCAG 6 cut(s) 22, 313, 404, 447, 489, 865
BseNI ACTGG 2 cut(s) 648, 999
BseRI GAGGAG 2 cut(s) 297, 649
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 3 cut(s) 125, 506, 922
BseYI CCCAGC 1 cut(s) 721
BshFI GGCC 5 cut(s) 323, 565, 615, 686, 692
BshNI GGYRCC 2 cut(s) 87, 749
BshVI ATCGAT 1 cut(s) 1042
BsiHKAI GWGCWC 1 cut(s) 434
BsiHKCI CYCGRG 1 cut(s) 566
BsiSI CCGG 9 cut(s) 209, 294, 314, 320, 425, 457, 525, 562, 964
BslI CCNNNNNNNGG 2 cut(s) 164, 201
BsnI GGCC 5 cut(s) 323, 565, 615, 686, 692
BsoBI CYCGRG 1 cut(s) 566
Bsp119I TTCGAA 1 cut(s) 946
Bsp1286I GDGCHC 5 cut(s) 90, 159, 434, 798, 870
Bsp13I TCCGGA 2 cut(s) 424, 963
Bsp143I GATC 8 cut(s) 204, 252, 277, 316, 459, 625, 927, 988
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 7 cut(s) 159, 175, 195, 311, 327, 367, 454
BspANI GGCC 5 cut(s) 323, 565, 615, 686, 692
BspCNI CTCAG 6 cut(s) 21, 312, 405, 446, 490, 864
BspDI ATCGAT 1 cut(s) 1042
BspEI TCCGGA 2 cut(s) 424, 963
BspLI GGNNCC 8 cut(s) 89, 156, 206, 284, 318, 423, 751, 795
BspMI ACCTGC 2 cut(s) 379, 562
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 6 cut(s) 199, 212, 311, 324, 467, 620
BspQI GCTCTTC 1 cut(s) 480
BspT104I TTCGAA 1 cut(s) 946
BspT107I GGYRCC 2 cut(s) 87, 749
BsrI ACTGG 2 cut(s) 648, 999
BssECI CCNNGG 7 cut(s) 72, 117, 162, 609, 629, 687, 877
BssMI GATC 8 cut(s) 204, 252, 277, 316, 459, 625, 927, 988
BssNI GRCGYC 3 cut(s) 39, 53, 998
BssT1I CCWWGG 3 cut(s) 609, 629, 877
Bst2UI CCWGG 3 cut(s) 119, 202, 688
Bst4CI ACNGT 1 cut(s) 716
Bst6I CTCTTC 1 cut(s) 480
BstACI GRCGYC 3 cut(s) 39, 53, 998
BstBI TTCGAA 1 cut(s) 946
BstC8I GCNNGC 5 cut(s) 21, 159, 291, 492, 617
BstDEI CTNAG 8 cut(s) 8, 299, 413, 433, 498, 694, 776, 851
BstDSI CCRYGG 2 cut(s) 72, 609
BstF5I GGATG 2 cut(s) 790, 857
BstH2I RGCGCY 2 cut(s) 8, 20
BstHHI GCGC 2 cut(s) 7, 19
BstKTI GATC 8 cut(s) 207, 255, 280, 319, 462, 628, 930, 991
BstMBI GATC 8 cut(s) 204, 252, 277, 316, 459, 625, 927, 988
BstMWI GCNNNNNNNGC 7 cut(s) 25, 119, 174, 462, 503, 658, 916
BstNI CCWGG 3 cut(s) 119, 202, 688
BstSCI CCNGG 4 cut(s) 117, 200, 207, 686
BstSLI GKGCMC 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 125, 506, 922
BstX2I RGATCY 4 cut(s) 204, 316, 459, 927
BstXI CCANNNNNNTGG 1 cut(s) 746
BstYI RGATCY 4 cut(s) 204, 316, 459, 927
Bsu15I ATCGAT 1 cut(s) 1042
Bsu36I CCTNAGG 1 cut(s) 851
BsuRI GGCC 5 cut(s) 323, 565, 615, 686, 692
BsuTUI ATCGAT 1 cut(s) 1042
BtgI CCRYGG 2 cut(s) 72, 609
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 790, 857
BtsIMutI CAGTG 1 cut(s) 56
BveI ACCTGC 2 cut(s) 379, 562
Cac8I GCNNGC 5 cut(s) 21, 159, 291, 492, 617
CfoI GCGC 2 cut(s) 7, 19
Cfr13I GGNCC 5 cut(s) 564, 613, 646, 684, 690
ClaI ATCGAT 1 cut(s) 1042
CseI GACGC 2 cut(s) 47, 987
Csp6I GTAC 1 cut(s) 952
CviAII CATG 2 cut(s) 610, 922
CviQI GTAC 1 cut(s) 952
DdeI CTNAG 8 cut(s) 8, 299, 413, 433, 498, 694, 776, 851
DpnI GATC 8 cut(s) 206, 254, 279, 318, 461, 627, 929, 990
DpnII GATC 8 cut(s) 204, 252, 277, 316, 459, 625, 927, 988
DraIII CACNNNGTG 1 cut(s) 749
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 480
EciI GGCGGA 4 cut(s) 164, 184, 300, 443
Ecl136II GAGCTC 1 cut(s) 432
Eco130I CCWWGG 3 cut(s) 609, 629, 877
Eco24I GRGCYC 4 cut(s) 159, 434, 798, 870
Eco47I GGWCC 1 cut(s) 646
Eco47III AGCGCT 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 432
Eco57I CTGAAG 1 cut(s) 422
Eco81I CCTNAGG 1 cut(s) 851
Eco88I CYCGRG 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 432
EcoO109I RGGNCCY 1 cut(s) 690
EcoRI GAATTC 1 cut(s) 982
EcoRII CCWGG 3 cut(s) 117, 200, 686
EcoT14I CCWWGG 3 cut(s) 609, 629, 877
EcoT38I GRGCYC 4 cut(s) 159, 434, 798, 870
ErhI CCWWGG 3 cut(s) 609, 629, 877
FaeI CATG 2 cut(s) 613, 925
FatI CATG 2 cut(s) 609, 921
FauI CCCGC 1 cut(s) 166
Fnu4HI GCNGC 3 cut(s) 114, 495, 911
FokI GGATG 2 cut(s) 797, 844
FriOI GRGCYC 4 cut(s) 159, 434, 798, 870
Fsp4HI GCNGC 3 cut(s) 114, 495, 911
FspBI CTAG 3 cut(s) 20, 83, 653
GlaI GCGC 2 cut(s) 6, 18
GluI GCNGC 3 cut(s) 114, 495, 911
GsaI CCCAGC 1 cut(s) 725
GsuI CTGGAG 2 cut(s) 269, 722
HaeII RGCGCY 2 cut(s) 8, 20
HaeIII GGCC 5 cut(s) 323, 565, 615, 686, 692
HapII CCGG 9 cut(s) 209, 294, 314, 320, 425, 457, 525, 562, 964
HgaI GACGC 2 cut(s) 47, 987
HhaI GCGC 2 cut(s) 7, 19
Hin1I GRCGYC 3 cut(s) 39, 53, 998
Hin1II CATG 2 cut(s) 613, 925
Hin6I GCGC 2 cut(s) 5, 17
HinP1I GCGC 2 cut(s) 5, 17
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 4 cut(s) 180, 297, 348, 521
HpaII CCGG 9 cut(s) 209, 294, 314, 320, 425, 457, 525, 562, 964
HphI GGTGA 4 cut(s) 61, 136, 716, 754
Hpy166II GTNNAC 3 cut(s) 32, 265, 707
Hpy188I TCNGA 8 cut(s) 11, 154, 233, 448, 545, 604, 981, 988
Hpy188III TCNNGA 5 cut(s) 425, 515, 739, 853, 964
Hpy8I GTNNAC 3 cut(s) 32, 265, 707
Hpy99I CGWCG 2 cut(s) 41, 1003
HpyAV CCTTC 3 cut(s) 397, 464, 661
HpyCH4III ACNGT 1 cut(s) 716
HpyCH4IV ACGT 2 cut(s) 53, 531
HpyCH4V TGCA 3 cut(s) 113, 578, 700
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 119, 174, 462, 503, 658, 916
HpyF3I CTNAG 8 cut(s) 8, 299, 413, 433, 498, 694, 776, 851
HpySE526I ACGT 2 cut(s) 53, 531
Hsp92I GRCGYC 3 cut(s) 39, 53, 998
Hsp92II CATG 2 cut(s) 613, 925
HspAI GCGC 2 cut(s) 5, 17
Kpn2I TCCGGA 2 cut(s) 424, 963
Kzo9I GATC 8 cut(s) 204, 252, 277, 316, 459, 625, 927, 988
LguI GCTCTTC 1 cut(s) 480
LmnI GCTCC 6 cut(s) 154, 246, 288, 772, 793, 966
Lsp1109I GCAGC 3 cut(s) 125, 506, 922
MaeI CTAG 3 cut(s) 20, 83, 653
MaeII ACGT 2 cut(s) 53, 531
MaeIII GTNAC 3 cut(s) 49, 54, 811
MalI GATC 8 cut(s) 206, 254, 279, 318, 461, 627, 929, 990
MboI GATC 8 cut(s) 204, 252, 277, 316, 459, 625, 927, 988
MboII GAAGA 4 cut(s) 175, 382, 497, 877
MflI RGATCY 4 cut(s) 204, 316, 459, 927
MhlI GDGCHC 5 cut(s) 90, 159, 434, 798, 870
MluCI AATT 2 cut(s) 597, 982
MlyI GAGTC 2 cut(s) 291, 530
MmeI TCCRAC 2 cut(s) 211, 913
MroI TCCGGA 2 cut(s) 424, 963
MroXI GAANNNNTTC 1 cut(s) 971
MseI TTAA 1 cut(s) 900
MspA1I CMGCKG 2 cut(s) 329, 497
MspI CCGG 9 cut(s) 209, 294, 314, 320, 425, 457, 525, 562, 964
MspR9I CCNGG 4 cut(s) 119, 202, 209, 688
MvaI CCWGG 3 cut(s) 119, 202, 688
MwoI GCNNNNNNNGC 7 cut(s) 25, 119, 174, 462, 503, 658, 916
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 609
NdeII GATC 8 cut(s) 204, 252, 277, 316, 459, 625, 927, 988
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 2 cut(s) 613, 925
NlaIV GGNNCC 8 cut(s) 89, 156, 206, 284, 318, 423, 751, 795
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 3 cut(s) 49, 54, 811
NspV TTCGAA 1 cut(s) 946
PaqCI CACCTGC 1 cut(s) 379
PciSI GCTCTTC 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 971
PfeI GAWTC 2 cut(s) 180, 348
PflFI GACNNNGTC 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 207
PkrI GCNGC 3 cut(s) 115, 496, 912
PleI GAGTC 2 cut(s) 291, 529
PpsI GAGTC 2 cut(s) 291, 529
Psp124BI GAGCTC 1 cut(s) 434
Psp6I CCWGG 3 cut(s) 117, 200, 686
PspFI CCCAGC 1 cut(s) 721
PspGI CCWGG 3 cut(s) 117, 200, 686
PspN4I GGNNCC 8 cut(s) 89, 156, 206, 284, 318, 423, 751, 795
PspPI GGNCC 5 cut(s) 564, 613, 646, 684, 690
PsrI GAACNNNNNNTAC 2 cut(s) 434, 466
PsuI RGATCY 4 cut(s) 204, 316, 459, 927
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 1 cut(s) 953
RsaNI GTAC 1 cut(s) 952
SacI GAGCTC 1 cut(s) 434
SapI GCTCTTC 1 cut(s) 480
SaqAI TTAA 1 cut(s) 900
SatI GCNGC 3 cut(s) 114, 495, 911
Sau3AI GATC 8 cut(s) 204, 252, 277, 316, 459, 625, 927, 988
Sau96I GGNCC 5 cut(s) 564, 613, 646, 684, 690
SchI GAGTC 2 cut(s) 291, 530
ScrFI CCNGG 4 cut(s) 119, 202, 209, 688
SduI GDGCHC 5 cut(s) 90, 159, 434, 798, 870
SfuI TTCGAA 1 cut(s) 946
SinI GGWCC 1 cut(s) 646
Sse9I AATT 2 cut(s) 597, 982
SsiI CCGC 7 cut(s) 159, 175, 195, 311, 327, 367, 454
SspMI CTAG 3 cut(s) 20, 83, 653
SstI GAGCTC 1 cut(s) 434
StyD4I CCNGG 4 cut(s) 117, 200, 207, 686
StyI CCWWGG 3 cut(s) 609, 629, 877
TaaI ACNGT 1 cut(s) 716
TaiI ACGT 2 cut(s) 56, 534
TaqI TCGA 4 cut(s) 36, 516, 946, 1042
TasI AATT 2 cut(s) 597, 982
TatI WGTACW 1 cut(s) 951
TfiI GAWTC 2 cut(s) 180, 348
Tru1I TTAA 1 cut(s) 900
Tru9I TTAA 1 cut(s) 900
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 3 cut(s) 49, 54, 811
TseI GCWGC 3 cut(s) 113, 494, 910
Tsp45I GTSAC 3 cut(s) 49, 54, 811
TspGWI ACGGA 5 cut(s) 61, 233, 288, 343, 360
TspRI CASTG 1 cut(s) 63
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 646
XapI RAATTY 1 cut(s) 982
XmnI GAANNNNTTC 1 cut(s) 971
XspI CTAG 3 cut(s) 20, 83, 653
ZraI GACGTC 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.