Rorug06G0296500

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012447
Physical Location & Seq
Forward (+)
44955347 .. 44959208
3862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0296500.1

Sequence Viewer

Length: 3375 bp
ATGAAAGCAGTTCAGGAGACGCCTCTGCGTCTCCTCCTCCTCCATATTTTCTTGCTCTTTTTGCTTCCATTGGAGGCCACGTCATCACAAAGAATACAAGCTGAAGCTCTCCTAAAATGGAAGAACAGCTTTACTTCTTCACCACCTTCCCTGAATTCATGGTCGCTCACAAACCTAAACAACCTGTGCAACTGGTCTGCCATTGTCTGCAACCACAATACCAAAACAGTTTCTGAAATTCACCTTGCAAACTTCAACATCACCGCAGCACTAACACAGTTCAACTTCAACAAGTTTTTCGATCTCACCCATTTCAACGTCAGTGGCAACAATTTCTCAGGGCCTATACCATCTGCCATTGGCAACCTCACCAAGCTCACAACCTTGGATTTGGGAAACAATACCTTGGAACGAGAAGTGCCTGCGGAAATGGGCAAGTTAACGGAGCTTCATTTTCTAAGTCTCAACAACAACTATCTCAGTGGTACCATTCCCTATCGGCTGGACAATCTCAAAAAGGTACAATATTTGCTTCTTGGAGGTAACCACTTAGACACTCCTGACTGGTCTAAATTTTCAGGCTTTCCTTTGTTGAGCTACCTTGATCTTTATGGGAACAATTTAGATTCAGAGTTCCCAGAATTTATATTTGAATGTAGGAACTTGACCTTCCTGGATTTATTTGGAAATCTTTTGACTGGCCAAATACCAAAATTGGTAGTTACCAACCTGGTCAAGCTTGAATCTCTCAATCTCACCGATAACCGATTCCAAGGGCCATTCCCATATAACTTTCCCAACATAAAGCACCTTTATTTGGAATCGAACAACTTTAGTGGTTCAATTCCTGAAGATATTGGTTTGATATCTGGTCTTCAATTTATTAGCCTTTATAACAATTCCCTTGAAGGCAAAATCCCATCCTCTTTAGGCCAAATCAGGGAGCTTCAGCACCTAGACCTTGGATGGAATTCCTTGAACTCTTCAATCCCTTCTGAGCTTGGTCTTTGTACCAACCTAACTTATTTGTCCTTGGCTATGAATAAACTCAGTGGGAAAATTCCAGCAGAAATTGGCCTGTTGACAAAACTTGATCTCCTCCTGTTGGGCGGAAACAATTTATCTGGATCAATTCCCTCAGAGATAGGAAACTTGAAAGTTTTGAGAAAATTGAACCTTTGGGGAAACCAGCTCTCAGGGCCGATTCCTATGAAATTATGGAACCTCACAAATCTTCAGAAGCTTTCTTTAGGAAACAATAGCTTCAATGGGAATATTCCACCAGAAATTGGCCTGTTGACAGAACTTGATATCCTCCGGCTGTTCAAAAACAATTTCTCTGGATCAATTCCCTCAGAGATAGGAAACTTGAAAGTTTTGAGAGAGTTGGACCTTTCGTGGAACCAGTTCACAGGGCCGATTCCTATGAAATTCTGGAACCTCACAAATCTTCAAAACATGGGCCTTTACAACAACAACCTTTCCGGCCCAATCCACCCAGAGATTGGAAACATGAGTTCCATGGTTGACTTTCATGTCAGCGTAAACCGACTTGAAGGGGAGTTGCCAAAAACTATTTCTCACCTCAGAAACCTAGAGGGGTTTTCCGTTTTCACTAACAGATTTTGGGGGACCATTCCCAGTGATTTCGGGAAGTATAGTCCAAACTTGACACTTATTCAGTTGGCATGGAACAGCTTCTCTGGAGAATTGCCGCCCGAGTTGTGTAGTGGATTTGCTTTGGAGAAGTTGATAGTGAACTCGAACAACTTCACAGGGCCATTGCCTGAGTGCTTGAGAAATTGTTCTGCATTAACACATGTCAGGCTTGAAGTAAACCAATTCACCGGAAACAATACCAATGCATTTGGTATTCATCCCCGTCTTGTATTAATTCATCTTAGTCATAACAAGTTCGTTGGTCAACTCTCGCCACAATGGGGAGAATGCAAAAACATCACGGATATGCAAATGGACGGAAATAGAATTTCTGGTCAAATCCCACCTGAGCTTGGGCAGTTAAGACAATTGCAGAAGCTAAACCTAGACTCTAATGAATTCAGTGGGGAATTTCCAGTTGCCATTGGGAATCTAAGCTTGTTGTTCAATCTCAGTCTCAGCAGGAACTACTTGACAGGAGTTATCCCTCGGTTTCTTGGCAAATTGACAAGGCTTGAGTTTCTTGATTTGTCTGATAATAATTTTACAGGCGTATTACCAGTCGACCCTGTAACGTTTGAGAGGTTAACAAGCTTGAACATAAGCCACAACAAATTATCAGGTGGAATACCAGAGGAGATTGGTAATCTACACCTGCAGTACTTGTTGGACCTAAGCAGCAATACATTCTCGGGACCACTACCATTAAACCTTGCCAAGCTTATAATGCTGGAGATTCTTAATGTCTCGCATAACCAACTCTCCGGGAATATCCCATCAACATTTTCCAGAATGGTTAGTCTCGTCGGCATTGATTTTTCTTACAACAACTTGACAGGTCCAATCCCAACTGGTGGCATTTTCCAAAAGAAGCTTCCGAATACTTTCATTGGAAATAGTGGTTTGTGTGGTGAAACAGAGGGACTCACTCCATGCACTTCGGGTCCCAAAAAGTCCAACAAGAAAATTCTTGTTGGCGTCCTTTTTTCGATTTGTGGTTTATTGCTGATGGTGACCATATCTGCTGTAATCCTTAAGTTCTGCAAGAAATCAAAGCCTGGAGAAATCGATCATCCTAAGATGTCTAAAAGTTTTGATTCAAGTATTTGGGGAAGACATGGTAAACTTACATTTTCTGAAATCATCACTGCCACTGAGAACTTTGATGAAAAGTACCTCATTGGAAGAGGAGGTTTTGGAAGTGTCTACAAGGCTGTGTTGGACAGGGGCAGAGTCGTTGCAGTTAAGAAATTGAATATGTCAGTAGATTCTAGTGACGACATTCCAGAATCGAATCGCAAGAGTTTTGAGAATGAGATAAGAACCTTGACAGAAGTGATGCACAGAAATGTAATAAAACTTTATGGGTTCTGTTCCTGGAAGGGGTGCTTGTACTTGGTGTATGAATATGCAGAGAGAGGCAGTTTGAAAATGGTATTGCATGGAGTGGAGGGCGAAGCAGAAGTACTTGGCTGGGACACAAGGGTGGAAATTGTGCAAGGAATAGCTCATGCTATTTCTCACTTGCATAATGACTGCTCTCACCCAATCGTTCACCGCGACATAACTTTGAGCAACATATTACTAGATCGGGATTTCGTGCCACGCCTATCAGACTTTGGAACAGCAAGACTATTGAGGTCAAATTCCTCAAACTGGACTGCTGTAGCAGGGACTTATGGCTACATGGCTCCAGCTTTGGGGTGGTGGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04218 ko04621 map04020 map04218 map04621
Pfam Domains
Protein Families
MCU

Protein Analysis

1124

Amino Acids

124.95

Weight (kDa)

6.85

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 71 6.8e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 97 - 178 3.3e-07 Leucine-rich repeat region
LRR_8 PF13855 173 - 232 5.9e-07 Leucine rich repeat
LRR_14 PF23598 268 - 367 1.9e-06 Leucine-rich repeat region
LRR_14 PF23598 333 - 442 2.3e-09 Leucine-rich repeat region
LRR_14 PF23598 448 - 639 1.1e-07 Leucine-rich repeat region
LRR_8 PF13855 675 - 735 9.5e-08 Leucine rich repeat
Pkinase PF00069 943 - 1118 1.1e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 946 - 1104 1.9e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42610
fragaria_vesca FvH4_2g40260
malus_domestica MD00G1134100.v1.1 MD08G1036100.v1.1 MD15G1032500.v1.1
prunus_persica Prupe.1G383500_v2.0.a1
pyrus_communis pycom08g02890 pycom15g02820
rosa_chinensis RchiOBHm_Chr6g0300501
rosa_laevigata RLG00000011332
rosa_multiflora Rmu_co8175312.1_g000001 Rmu_sc0001476.1_g000012
rosa_roxburghii Rroxscaffold_7G00167730
rosa_rugosa Rorug06G0296500
rosa_samantha Rh6AG409100 Rh6BG416400 Rh6CG424000 Rh6DG409600
rosa_wichuraiana Rw6G035800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 894, 2384
AarI CACCTGC 1 cut(s) 2322
AasI GACNNNNNNGTC 1 cut(s) 1535
Acc36I ACCTGC 1 cut(s) 2322
Acc65I GGTACC 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 485
AccB7I CCANNNNNTGG 3 cut(s) 1289, 1505, 2513
AccI GTMKAC 2 cut(s) 2223, 2865
AccII CGCG 1 cut(s) 3219
AciI CCGC 5 cut(s) 264, 425, 1110, 1715, 3217
AclI AACGTT 1 cut(s) 2234
AclWI GGATC 2 cut(s) 1135, 1351
AcoI YGGCCR 1 cut(s) 700
AcuI CTGAAG 4 cut(s) 123, 870, 932, 1220
AcyI GRCGYC 2 cut(s) 20, 2637
AfaI GTAC 7 cut(s) 487, 522, 1012, 2321, 2834, 3053, 3126
AflII CTTAAG 1 cut(s) 2693
AflIII ACRYGT 1 cut(s) 1819
AjiI CACGTC 1 cut(s) 81
AjnI CCWGG 4 cut(s) 672, 729, 2716, 3035
AjuI GAANNNNNNNTTGG 4 cut(s) 1561, 1593, 2517, 2549
AleI CACNNNNGTG 1 cut(s) 3143
AloI GAACNNNNNNTCC 2 cut(s) 1296, 1328
Alw26I GTCTC 6 cut(s) 11, 35, 467, 2120, 2410, 2465
AlwI GGATC 2 cut(s) 1135, 1351
AlwNI CAGNNNCTG 1 cut(s) 233
Ama87I CYCGRG 2 cut(s) 1718, 2350
ApeKI GCWGC 2 cut(s) 266, 2337
AseI ATTAAT 1 cut(s) 1892
Asp700I GAANNNNTTC 5 cut(s) 1406, 1697, 1769, 1804, 2543
Asp718I GGTACC 1 cut(s) 485
AsuC2I CCSGG 1 cut(s) 2425
AvaI CYCGRG 2 cut(s) 1718, 2350
AvaII GGWCC 6 cut(s) 1390, 1632, 2329, 2354, 2498, 2603
BalI TGGCCA 1 cut(s) 702
BanI GGYRCC 1 cut(s) 485
BarI GAAGNNNNNNTAC 2 cut(s) 3108, 3140
BbsI GAAGAC 2 cut(s) 866, 2779
BbvI GCAGC 2 cut(s) 278, 2349
BccI CCATC 5 cut(s) 358, 928, 960, 2443, 2662
BciT130I CCWGG 4 cut(s) 674, 731, 2718, 3037
BcnI CCSGG 1 cut(s) 2425
BcoDI GTCTC 6 cut(s) 11, 35, 467, 2120, 2410, 2465
BfaI CTAG 5 cut(s) 956, 1595, 2045, 2931, 3245
BfmI CTRYAG 2 cut(s) 2315, 3324
BfrI CTTAAG 1 cut(s) 2693
BfuAI ACCTGC 1 cut(s) 2322
BisI GCNGC 3 cut(s) 267, 1715, 2338
BlsI GCNGC 3 cut(s) 268, 1716, 2339
BmcAI AGTACT 2 cut(s) 2321, 3126
Bme1390I CCNGG 5 cut(s) 674, 731, 2425, 2718, 3037
Bme18I GGWCC 6 cut(s) 1390, 1632, 2329, 2354, 2498, 2603
BmeT110I CYCGRG 2 cut(s) 1718, 2350
BmgBI CACGTC 1 cut(s) 81
BmiI GGNNCC 9 cut(s) 487, 1223, 1403, 1439, 1633, 2355, 2604, 2605, 3351
BmrFI CCNGG 5 cut(s) 674, 731, 2425, 2718, 3037
BmrI ACTGGG 1 cut(s) 1635
BmsI GCATC 1 cut(s) 2988
BmuI ACTGGG 1 cut(s) 1635
BpiI GAAGAC 2 cut(s) 866, 2779
BpmI CTGGAG 4 cut(s) 1725, 2411, 2739, 3336
Bpu10I CCTNAGC 2 cut(s) 2007, 2333
BpuEI CTTGAG 2 cut(s) 1816, 2195
BpuMI CCSGG 1 cut(s) 2425
Bsa29I ATCGAT 1 cut(s) 2727
BsaHI GRCGYC 2 cut(s) 20, 2637
BsaJI CCNNGG 7 cut(s) 384, 405, 772, 961, 1032, 1521, 2147
BsaWI WCCGGW 1 cut(s) 1847
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 1080, 1110, 2405, 2435
Bse1I ACTGG 9 cut(s) 197, 569, 703, 1405, 1641, 2075, 2219, 2515, 3320
Bse3DI GCAATG 1 cut(s) 1781
BseBI CCWGG 4 cut(s) 674, 731, 2718, 3037
BseCI ATCGAT 1 cut(s) 2727
BseDI CCNNGG 7 cut(s) 384, 405, 772, 961, 1032, 1521, 2147
BseGI GGATG 4 cut(s) 920, 971, 1876, 2731
BseMI GCAATG 1 cut(s) 1781
BseNI ACTGG 9 cut(s) 197, 569, 703, 1405, 1641, 2075, 2219, 2515, 3320
BseRI GAGGAG 6 cut(s) 23, 26, 29, 1088, 2309, 2862
BseXI GCAGC 2 cut(s) 278, 2349
BseYI CCCAGC 1 cut(s) 3132
Bsh1236I CGCG 1 cut(s) 3219
BshNI GGYRCC 1 cut(s) 485
BshVI ATCGAT 1 cut(s) 2727
BsiHKCI CYCGRG 2 cut(s) 1718, 2350
BsiSI CCGG 4 cut(s) 1318, 1485, 1848, 2424
BslFI GGGAC 6 cut(s) 1645, 2367, 2589, 2595, 3149, 3346
BsmAI GTCTC 6 cut(s) 11, 35, 467, 2120, 2410, 2465
BsmBI CGTCTC 2 cut(s) 11, 35
BsmFI GGGAC 6 cut(s) 1645, 2367, 2589, 2595, 3149, 3346
BsmI GAATGC 1 cut(s) 1952
BsoBI CYCGRG 2 cut(s) 1718, 2350
Bsp143I GATC 7 cut(s) 301, 604, 1093, 1127, 1343, 2728, 3247
Bsp19I CCATGG 1 cut(s) 1521
BspACI CCGC 5 cut(s) 264, 425, 1110, 1715, 3217
BspDI ATCGAT 1 cut(s) 2727
BspFNI CGCG 1 cut(s) 3219
BspLI GGNNCC 9 cut(s) 487, 1223, 1403, 1439, 1633, 2355, 2604, 2605, 3351
BspMAI CTGCAG 1 cut(s) 2319
BspMI ACCTGC 1 cut(s) 2322
BspPI GGATC 2 cut(s) 1135, 1351
BspT107I GGYRCC 1 cut(s) 485
BspTI CTTAAG 1 cut(s) 2693
BsrDI GCAATG 1 cut(s) 1781
BsrI ACTGG 9 cut(s) 197, 569, 703, 1405, 1641, 2075, 2219, 2515, 3320
BssECI CCNNGG 7 cut(s) 384, 405, 772, 961, 1032, 1521, 2147
BssMI GATC 7 cut(s) 301, 604, 1093, 1127, 1343, 2728, 3247
BssNI GRCGYC 2 cut(s) 20, 2637
BssT1I CCWWGG 6 cut(s) 384, 405, 772, 961, 1032, 1521
Bst2UI CCWGG 4 cut(s) 674, 731, 2718, 3037
Bst4CI ACNGT 2 cut(s) 229, 279
Bst6I CTCTTC 2 cut(s) 988, 2839
BstACI GRCGYC 2 cut(s) 20, 2637
BstAFI CTTAAG 1 cut(s) 2693
BstC8I GCNNGC 1 cut(s) 423
BstDSI CCRYGG 1 cut(s) 1521
BstEII GGTNACC 2 cut(s) 542, 2671
BstF5I GGATG 4 cut(s) 920, 971, 1876, 2731
BstFNI CGCG 1 cut(s) 3219
BstKTI GATC 7 cut(s) 304, 607, 1096, 1130, 1346, 2731, 3250
BstMAI GTCTC 6 cut(s) 11, 35, 467, 2120, 2410, 2465
BstMBI GATC 7 cut(s) 301, 604, 1093, 1127, 1343, 2728, 3247
BstMWI GCNNNNNNNGC 3 cut(s) 61, 1198, 2386
BstNI CCWGG 4 cut(s) 674, 731, 2718, 3037
BstNSI RCATGY 1 cut(s) 1823
BstPI GGTNACC 2 cut(s) 542, 2671
BstSCI CCNGG 5 cut(s) 672, 729, 2423, 2716, 3035
BstSFI CTRYAG 2 cut(s) 2315, 3324
BstUI CGCG 1 cut(s) 3219
BstV1I GCAGC 2 cut(s) 278, 2349
BstV2I GAAGAC 2 cut(s) 866, 2779
Bsu15I ATCGAT 1 cut(s) 2727
BsuTUI ATCGAT 1 cut(s) 2727
BtgI CCRYGG 1 cut(s) 1521
BtrI CACGTC 1 cut(s) 81
BtsCI GGATG 4 cut(s) 920, 971, 1876, 2731
BtsI GCAGTG 1 cut(s) 2805
BtsIMutI CAGTG 7 cut(s) 328, 487, 1057, 1648, 2068, 2805, 2811
BveI ACCTGC 1 cut(s) 2322
Cac8I GCNNGC 1 cut(s) 423
CaiI CAGNNNCTG 1 cut(s) 233
ClaI ATCGAT 1 cut(s) 2727
CseI GACGC 3 cut(s) 17, 28, 2626
CsiI ACCWGGT 1 cut(s) 729
Csp6I GTAC 7 cut(s) 486, 521, 1011, 2320, 2833, 3052, 3125
CspCI CAANNNNNGTGG 4 cut(s) 304, 339, 817, 852
CviQI GTAC 7 cut(s) 486, 521, 1011, 2320, 2833, 3052, 3125
DpnI GATC 7 cut(s) 303, 606, 1095, 1129, 1345, 2730, 3249
DpnII GATC 7 cut(s) 301, 604, 1093, 1127, 1343, 2728, 3247
DrdI GACNNNNNNGTC 1 cut(s) 1535
DseDI GACNNNNNNGTC 1 cut(s) 1535
EaeI YGGCCR 1 cut(s) 700
Eam1104I CTCTTC 2 cut(s) 988, 2839
EarI CTCTTC 2 cut(s) 988, 2839
EciI GGCGGA 1 cut(s) 1125
Eco130I CCWWGG 6 cut(s) 384, 405, 772, 961, 1032, 1521
Eco32I GATATC 2 cut(s) 867, 1312
Eco47I GGWCC 6 cut(s) 1390, 1632, 2329, 2354, 2498, 2603
Eco57I CTGAAG 4 cut(s) 123, 870, 932, 1220
Eco88I CYCGRG 2 cut(s) 1718, 2350
Eco91I GGTNACC 2 cut(s) 542, 2671
EcoO109I RGGNCCY 2 cut(s) 341, 2603
EcoO65I GGTNACC 2 cut(s) 542, 2671
EcoRI GAATTC 3 cut(s) 154, 970, 2057
EcoRII CCWGG 4 cut(s) 672, 729, 2716, 3035
EcoRV GATATC 2 cut(s) 867, 1312
EcoT14I CCWWGG 6 cut(s) 384, 405, 772, 961, 1032, 1521
EcoT22I ATGCAT 1 cut(s) 1867
ErhI CCWWGG 6 cut(s) 384, 405, 772, 961, 1032, 1521
Esp3I CGTCTC 2 cut(s) 11, 35
FalI AAGNNNNNCTT 4 cut(s) 113, 145, 3032, 3064
FaqI GGGAC 6 cut(s) 1645, 2367, 2589, 2595, 3149, 3346
FblI GTMKAC 2 cut(s) 2223, 2865
Fnu4HI GCNGC 3 cut(s) 267, 1715, 2338
FokI GGATG 4 cut(s) 907, 978, 1863, 2718
Fsp4HI GCNGC 3 cut(s) 267, 1715, 2338
FspBI CTAG 5 cut(s) 956, 1595, 2045, 2931, 3245
GluI GCNGC 3 cut(s) 267, 1715, 2338
GsaI CCCAGC 1 cut(s) 3136
GsuI CTGGAG 4 cut(s) 1725, 2411, 2739, 3336
HapII CCGG 4 cut(s) 1318, 1485, 1848, 2424
HgaI GACGC 3 cut(s) 17, 28, 2626
Hin1I GRCGYC 2 cut(s) 20, 2637
HincII GTYRAC 7 cut(s) 441, 1083, 1299, 1528, 1925, 2224, 2247
HindII GTYRAC 7 cut(s) 441, 1083, 1299, 1528, 1925, 2224, 2247
HindIII AAGCTT 6 cut(s) 737, 1241, 2095, 2251, 2378, 2531
HpaI GTTAAC 2 cut(s) 441, 2247
HpaII CCGG 4 cut(s) 1318, 1485, 1848, 2424
Hpy99I CGWCG 1 cut(s) 2468
HpyAV CCTTC 6 cut(s) 156, 679, 902, 1002, 1550, 3034
HpyCH4III ACNGT 2 cut(s) 229, 279
HpyCH4IV ACGT 3 cut(s) 80, 318, 2234
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 1198, 2386
HpySE526I ACGT 3 cut(s) 80, 318, 2234
Hsp92I GRCGYC 2 cut(s) 20, 2637
KflI GGGWCCC 1 cut(s) 2603
KpnI GGTACC 1 cut(s) 489
KspAI GTTAAC 2 cut(s) 441, 2247
Kzo9I GATC 7 cut(s) 301, 604, 1093, 1127, 1343, 2728, 3247
LmnI GCTCC 3 cut(s) 445, 943, 3355
Lsp1109I GCAGC 2 cut(s) 278, 2349
LweI GCATC 1 cut(s) 2988
MabI ACCWGGT 1 cut(s) 729
MaeI CTAG 5 cut(s) 956, 1595, 2045, 2931, 3245
MaeII ACGT 3 cut(s) 80, 318, 2234
MaeIII GTNAC 5 cut(s) 542, 721, 2230, 2671, 2933
MalI GATC 7 cut(s) 303, 606, 1095, 1129, 1345, 2730, 3249
MboI GATC 7 cut(s) 301, 604, 1093, 1127, 1343, 2728, 3247
MboII GAAGA 9 cut(s) 129, 133, 863, 866, 975, 1226, 1442, 2784, 2856
MfeI CAATTG 1 cut(s) 2027
MlsI TGGCCA 1 cut(s) 702
MluNI TGGCCA 1 cut(s) 702
MlyI GAGTC 3 cut(s) 2042, 2577, 2901
MmeI TCCRAC 4 cut(s) 1368, 2307, 2640, 2859
Mox20I TGGCCA 1 cut(s) 702
Mph1103I ATGCAT 1 cut(s) 1867
MroXI GAANNNNTTC 5 cut(s) 1406, 1697, 1769, 1804, 2543
MscI TGGCCA 1 cut(s) 702
MseI TTAA 9 cut(s) 440, 1814, 1892, 2021, 2246, 2366, 2400, 2694, 2904
MslI CAYNNNNRTG 3 cut(s) 1964, 3006, 3143
Msp20I TGGCCA 1 cut(s) 702
MspCI CTTAAG 1 cut(s) 2693
MspI CCGG 4 cut(s) 1318, 1485, 1848, 2424
MspR9I CCNGG 5 cut(s) 674, 731, 2425, 2718, 3037
MunI CAATTG 1 cut(s) 2027
Mva1269I GAATGC 1 cut(s) 1952
MvaI CCWGG 4 cut(s) 674, 731, 2718, 3037
MvnI CGCG 1 cut(s) 3219
MwoI GCNNNNNNNGC 3 cut(s) 61, 1198, 2386
NciI CCSGG 1 cut(s) 2425
NcoI CCATGG 1 cut(s) 1521
NdeII GATC 7 cut(s) 301, 604, 1093, 1127, 1343, 2728, 3247
NlaIV GGNNCC 9 cut(s) 487, 1223, 1403, 1439, 1633, 2355, 2604, 2605, 3351
NmuCI GTSAC 2 cut(s) 2671, 2933
NsiI ATGCAT 1 cut(s) 1867
NspI RCATGY 1 cut(s) 1823
OliI CACNNNNGTG 1 cut(s) 3143
PaqCI CACCTGC 1 cut(s) 2322
PciI ACATGT 1 cut(s) 1819
PcsI WCGNNNNNNNCGW 1 cut(s) 3216
PctI GAATGC 1 cut(s) 1952
PdmI GAANNNNTTC 5 cut(s) 1406, 1697, 1769, 1804, 2543
PflMI CCANNNNNTGG 3 cut(s) 1289, 1505, 2513
PfoI TCCNGGA 3 cut(s) 672, 2423, 3035
PkrI GCNGC 3 cut(s) 268, 1716, 2339
PleI GAGTC 3 cut(s) 2042, 2577, 2900
PpsI GAGTC 3 cut(s) 2042, 2577, 2900
PpuMI RGGWCCY 1 cut(s) 2603
PscI ACATGT 1 cut(s) 1819
PshBI ATTAAT 1 cut(s) 1892
PsiI TTATAA 2 cut(s) 894, 2384
Psp1406I AACGTT 1 cut(s) 2234
Psp5II RGGWCCY 1 cut(s) 2603
Psp6I CCWGG 4 cut(s) 672, 729, 2716, 3035
PspEI GGTNACC 2 cut(s) 542, 2671
PspFI CCCAGC 1 cut(s) 3132
PspGI CCWGG 4 cut(s) 672, 729, 2716, 3035
PspN4I GGNNCC 9 cut(s) 487, 1223, 1403, 1439, 1633, 2355, 2604, 2605, 3351
PspPPI RGGWCCY 1 cut(s) 2603
PstI CTGCAG 1 cut(s) 2319
PstNI CAGNNNCTG 1 cut(s) 233
RsaI GTAC 7 cut(s) 487, 522, 1012, 2321, 2834, 3053, 3126
RsaNI GTAC 7 cut(s) 486, 521, 1011, 2320, 2833, 3052, 3125
RseI CAYNNNNRTG 3 cut(s) 1964, 3006, 3143
SalI GTCGAC 1 cut(s) 2222
SaqAI TTAA 9 cut(s) 440, 1814, 1892, 2021, 2246, 2366, 2400, 2694, 2904
SatI GCNGC 3 cut(s) 267, 1715, 2338
Sau3AI GATC 7 cut(s) 301, 604, 1093, 1127, 1343, 2728, 3247
ScaI AGTACT 2 cut(s) 2321, 3126
SchI GAGTC 3 cut(s) 2042, 2577, 2901
ScrFI CCNGG 5 cut(s) 674, 731, 2425, 2718, 3037
SexAI ACCWGGT 1 cut(s) 729
SfaNI GCATC 1 cut(s) 2988
SfcI CTRYAG 2 cut(s) 2315, 3324
SinI GGWCC 6 cut(s) 1390, 1632, 2329, 2354, 2498, 2603
SmiMI CAYNNNNRTG 3 cut(s) 1964, 3006, 3143
SmlI CTYRAG 3 cut(s) 1795, 2174, 2693
SmoI CTYRAG 3 cut(s) 1795, 2174, 2693
SsiI CCGC 5 cut(s) 264, 425, 1110, 1715, 3217
SspI AATATT 2 cut(s) 527, 1276
SspMI CTAG 5 cut(s) 956, 1595, 2045, 2931, 3245
StyD4I CCNGG 5 cut(s) 672, 729, 2423, 2716, 3035
StyI CCWWGG 6 cut(s) 384, 405, 772, 961, 1032, 1521
TaaI ACNGT 2 cut(s) 229, 279
TaiI ACGT 3 cut(s) 83, 321, 2237
TaqI TCGA 7 cut(s) 300, 824, 1763, 2223, 2648, 2727, 2951
TatI WGTACW 3 cut(s) 2319, 3051, 3124
TauI GCSGC 1 cut(s) 1717
Tru1I TTAA 9 cut(s) 440, 1814, 1892, 2021, 2246, 2366, 2400, 2694, 2904
Tru9I TTAA 9 cut(s) 440, 1814, 1892, 2021, 2246, 2366, 2400, 2694, 2904
TscAI CASTG 7 cut(s) 328, 487, 1057, 1648, 2068, 2812, 2818
TseFI GTSAC 2 cut(s) 2671, 2933
TseI GCWGC 2 cut(s) 266, 2337
Tsp45I GTSAC 2 cut(s) 2671, 2933
TspGWI ACGGA 4 cut(s) 458, 1597, 1976, 1992
TspRI CASTG 7 cut(s) 328, 487, 1057, 1648, 2068, 2812, 2818
Van91I CCANNNNNTGG 3 cut(s) 1289, 1505, 2513
Vha464I CTTAAG 1 cut(s) 2693
VpaK11BI GGWCC 6 cut(s) 1390, 1632, 2329, 2354, 2498, 2603
VspI ATTAAT 1 cut(s) 1892
XceI RCATGY 1 cut(s) 1823
XcmI CCANNNNNNNNNTGG 2 cut(s) 1502, 3360
XmiI GTMKAC 2 cut(s) 2223, 2865
XmnI GAANNNNTTC 5 cut(s) 1406, 1697, 1769, 1804, 2543
XspI CTAG 5 cut(s) 956, 1595, 2045, 2931, 3245
ZrmI AGTACT 2 cut(s) 2321, 3126
Zsp2I ATGCAT 1 cut(s) 1867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.