RchiOBHm_Chr6g0300501

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012447
Physical Location & Seq
Reverse (-)
61331294 .. 61333062
1769 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26987

Sequence Viewer

Length: 1050 bp
ATGGCGCTCAGAAAAGCGCTAGCCAAGCGTTCACTCGACGCCGTCAGAGTGACGTCACTGACACCATTCTCCGTGGTCGTTCTAGGGCACCAAACCATTGTTCCACCTAATGCAGCCCAGGCAAACTTCCACAGGGAGTATCTCACCTCATCGGAGCCCGCCGAGAAAGGCTTTTTCCGGCGATTCTTCCACCACTCGGCCACCAGGATCCCGGAGTTCCTGTCCCTGCCTGTCGGAGAGAAGCTCCGTGAAAAGATCAAAGGTGTGAACAGGAACGGAGATCGGCTCCGGCTCGCCGGACTCAGCCCTCCACCGCCGGATCCGGCCACCGCTGAAGAGTCGTTTTACGGAATCTCCGTCAGCGATGTGCGAAAGATTATGAGGCTGTCGCAGGTGGAGAAGCTGAAGGCGAAGCTGAGAGAGGTTCCGGAGAGCTCCGTTTCGTATTCGGAGTTCCGCCGGATCTGCGTGGAAGGTTGCGAGAGCGAAGAGCAAGCAGCTGAGTTTGCGAAGCTTCTCGATGAGTCCGGCAACGTCATCGTTCTCGGAAATGTTGTGTTTCTCCGGCCCGAGCAGGTTGCAAAATCAATGGAGAGCATAATTTGTCAAACCATGGGCCTGCCAAACGATCCAAGGAGGAGAGAACTGGACCAGCTAGAGAAGCAAAAGAAGGTAATAGACCAAAGGGCCAGGGCCTTGGTGCAGGGTGAACTCTACGGTGGGCTGGGTTTTATACTACTCCAGACACTTGGTGCCATAAGGCTCACCTTTTGGGAGCTAAGTTGGGATGTGATGGAGCCCATTTGCTTCTTTGTGACCTCCATCTACTTTGCTATGGGCTACGGCTTTTTCCTCAGGACATCCACAGAGCCCACTTTCCAAGGGTTCTTCCAACGCCGTTTTAAGACCAAGCAGCAACGGCTCATGGAGATCTATAACTTTGATGTTCGAAAGTACAACCAGCTCCGGAAAGTGTTTTATCCGAATTCCGATCAACTGGCGTCTTTAGATTATGTAGAAACAACCCTTGTTGGTGCTTTACATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04218 ko04621 map04020 map04218 map04621
Pfam Domains
Protein Families
MCU

Protein Analysis

349

Amino Acids

39.89

Weight (kDa)

9.34

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGD-D PF06050 80 - 225 1e-05 2-hydroxyglutaryl-CoA dehydratase, D-component
MCU PF04678 163 - 322 7.9e-48 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42610
fragaria_vesca FvH4_2g40260
malus_domestica MD00G1134100.v1.1 MD08G1036100.v1.1 MD15G1032500.v1.1
prunus_persica Prupe.1G383500_v2.0.a1
pyrus_communis pycom08g02890 pycom15g02820
rosa_chinensis RchiOBHm_Chr6g0300501
rosa_laevigata RLG00000011332
rosa_multiflora Rmu_co8175312.1_g000001 Rmu_sc0001476.1_g000012
rosa_roxburghii Rroxscaffold_7G00167730
rosa_rugosa Rorug06G0296500
rosa_samantha Rh6AG409100 Rh6BG416400 Rh6CG424000 Rh6DG409600
rosa_wichuraiana Rw6G035800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 382
AatII GACGTC 1 cut(s) 56
Acc36I ACCTGC 2 cut(s) 382, 565
AccB1I GGYRCC 2 cut(s) 87, 752
AccIII TCCGGA 2 cut(s) 427, 966
AciI CCGC 4 cut(s) 159, 314, 330, 457
AclWI GGATC 6 cut(s) 202, 215, 314, 327, 470, 623
AcoI YGGCCR 2 cut(s) 198, 324
AcsI RAATTY 1 cut(s) 985
AcuI CTGAAG 2 cut(s) 354, 425
AcyI GRCGYC 3 cut(s) 39, 53, 1001
AdeI CACNNNGTG 1 cut(s) 752
AfaI GTAC 1 cut(s) 956
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 1 cut(s) 196
AjnI CCWGG 3 cut(s) 117, 203, 689
AjuI GAANNNNNNNTTGG 2 cut(s) 84, 116
AluBI AGCT 9 cut(s) 244, 403, 415, 435, 500, 514, 655, 778, 964
AluI AGCT 9 cut(s) 244, 403, 415, 435, 500, 514, 655, 778, 964
Alw21I GWGCWC 1 cut(s) 437
AlwI GGATC 6 cut(s) 202, 215, 314, 327, 470, 623
Ama87I CYCGRG 1 cut(s) 569
Aor13HI TCCGGA 2 cut(s) 427, 966
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 6 cut(s) 198, 324, 566, 616, 687, 693
ApeKI GCWGC 3 cut(s) 113, 497, 913
ApoI RAATTY 1 cut(s) 985
AspLEI GCGC 2 cut(s) 7, 19
AspS9I GGNCC 5 cut(s) 567, 616, 649, 687, 693
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 3 cut(s) 136, 719, 757
AsuII TTCGAA 1 cut(s) 949
AsuNHI GCTAGC 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 1 cut(s) 649
AxyI CCTNAGG 1 cut(s) 854
BaeGI GKGCMC 1 cut(s) 90
BamHI GGATCC 2 cut(s) 207, 319
BanI GGYRCC 2 cut(s) 87, 752
BanII GRGCYC 4 cut(s) 159, 437, 801, 873
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 3 cut(s) 125, 509, 925
BccI CCATC 2 cut(s) 787, 830
BceAI ACGGC 4 cut(s) 26, 859, 882, 935
BcgI CGANNNNNNTGC 4 cut(s) 520, 554, 560, 594
BciT130I CCWGG 3 cut(s) 119, 205, 691
BcnI CCSGG 1 cut(s) 212
BfaI CTAG 3 cut(s) 20, 83, 656
BfoI RGCGCY 2 cut(s) 8, 20
BfuAI ACCTGC 2 cut(s) 382, 565
BglII AGATCT 1 cut(s) 930
BisI GCNGC 3 cut(s) 114, 498, 914
BlsI GCNGC 3 cut(s) 115, 499, 915
Bme1390I CCNGG 4 cut(s) 119, 205, 212, 691
Bme18I GGWCC 1 cut(s) 649
BmeT110I CYCGRG 1 cut(s) 569
BmgT120I GGNCC 5 cut(s) 567, 616, 649, 687, 693
BmiI GGNNCC 8 cut(s) 89, 156, 209, 287, 321, 426, 754, 798
BmrFI CCNGG 4 cut(s) 119, 205, 212, 691
BmtI GCTAGC 1 cut(s) 23
BplI GAGNNNNNCTC 4 cut(s) 228, 260, 270, 302
BpmI CTGGAG 1 cut(s) 725
Bpu14I TTCGAA 1 cut(s) 949
BpuMI CCSGG 1 cut(s) 212
Bsa29I ATCGAT 1 cut(s) 1045
BsaHI GRCGYC 3 cut(s) 39, 53, 1001
BsaJI CCNNGG 7 cut(s) 72, 117, 612, 632, 690, 696, 880
BsaWI WCCGGW 2 cut(s) 427, 966
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 2 cut(s) 651, 1002
Bse21I CCTNAGG 1 cut(s) 854
BseAI TCCGGA 2 cut(s) 427, 966
BseBI CCWGG 3 cut(s) 119, 205, 691
BseCI ATCGAT 1 cut(s) 1045
BseDI CCNNGG 7 cut(s) 72, 117, 612, 632, 690, 696, 880
BseGI GGATG 2 cut(s) 793, 860
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 5 cut(s) 22, 316, 407, 492, 868
BseNI ACTGG 2 cut(s) 651, 1002
BseRI GAGGAG 1 cut(s) 652
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 3 cut(s) 125, 509, 925
BseYI CCCAGC 1 cut(s) 724
BsgI GTGCAG 1 cut(s) 722
BshFI GGCC 6 cut(s) 200, 326, 568, 618, 689, 695
BshNI GGYRCC 2 cut(s) 87, 752
BshVI ATCGAT 1 cut(s) 1045
BsiHKAI GWGCWC 1 cut(s) 437
BsiHKCI CYCGRG 1 cut(s) 569
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 208
BsnI GGCC 6 cut(s) 200, 326, 568, 618, 689, 695
BsoBI CYCGRG 1 cut(s) 569
Bsp119I TTCGAA 1 cut(s) 949
Bsp1286I GDGCHC 5 cut(s) 90, 159, 437, 801, 873
Bsp13I TCCGGA 2 cut(s) 427, 966
Bsp143I GATC 8 cut(s) 207, 255, 280, 319, 462, 628, 930, 991
Bsp19I CCATGG 1 cut(s) 612
BspACI CCGC 4 cut(s) 159, 314, 330, 457
BspANI GGCC 6 cut(s) 200, 326, 568, 618, 689, 695
BspCNI CTCAG 5 cut(s) 21, 315, 408, 493, 867
BspDI ATCGAT 1 cut(s) 1045
BspEI TCCGGA 2 cut(s) 427, 966
BspLI GGNNCC 8 cut(s) 89, 156, 209, 287, 321, 426, 754, 798
BspMI ACCTGC 2 cut(s) 382, 565
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 6 cut(s) 202, 215, 314, 327, 470, 623
BspQI GCTCTTC 1 cut(s) 483
BspT104I TTCGAA 1 cut(s) 949
BspT107I GGYRCC 2 cut(s) 87, 752
BsrI ACTGG 2 cut(s) 651, 1002
BssECI CCNNGG 7 cut(s) 72, 117, 612, 632, 690, 696, 880
BssMI GATC 8 cut(s) 207, 255, 280, 319, 462, 628, 930, 991
BssNI GRCGYC 3 cut(s) 39, 53, 1001
BssT1I CCWWGG 4 cut(s) 612, 632, 696, 880
Bst2UI CCWGG 3 cut(s) 119, 205, 691
Bst4CI ACNGT 1 cut(s) 719
Bst6I CTCTTC 2 cut(s) 330, 483
BstACI GRCGYC 3 cut(s) 39, 53, 1001
BstBI TTCGAA 1 cut(s) 949
BstC8I GCNNGC 5 cut(s) 21, 159, 294, 495, 620
BstDEI CTNAG 6 cut(s) 8, 302, 416, 501, 779, 854
BstDSI CCRYGG 2 cut(s) 72, 612
BstF5I GGATG 2 cut(s) 793, 860
BstH2I RGCGCY 2 cut(s) 8, 20
BstHHI GCGC 2 cut(s) 7, 19
BstKTI GATC 8 cut(s) 210, 258, 283, 322, 465, 631, 933, 994
BstMBI GATC 8 cut(s) 207, 255, 280, 319, 462, 628, 930, 991
BstMWI GCNNNNNNNGC 6 cut(s) 25, 119, 465, 506, 661, 919
BstNI CCWGG 3 cut(s) 119, 205, 691
BstSCI CCNGG 4 cut(s) 117, 203, 210, 689
BstSLI GKGCMC 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 125, 509, 925
BstX2I RGATCY 4 cut(s) 207, 319, 462, 930
BstXI CCANNNNNNTGG 2 cut(s) 697, 749
BstYI RGATCY 4 cut(s) 207, 319, 462, 930
Bsu15I ATCGAT 1 cut(s) 1045
Bsu36I CCTNAGG 1 cut(s) 854
BsuRI GGCC 6 cut(s) 200, 326, 568, 618, 689, 695
BsuTUI ATCGAT 1 cut(s) 1045
BtgI CCRYGG 2 cut(s) 72, 612
BtgZI GCGATG 1 cut(s) 378
BtsCI GGATG 2 cut(s) 793, 860
BtsIMutI CAGTG 1 cut(s) 56
BveI ACCTGC 2 cut(s) 382, 565
Cac8I GCNNGC 5 cut(s) 21, 159, 294, 495, 620
CfoI GCGC 2 cut(s) 7, 19
Cfr13I GGNCC 5 cut(s) 567, 616, 649, 687, 693
ClaI ATCGAT 1 cut(s) 1045
CseI GACGC 2 cut(s) 47, 990
Csp6I GTAC 1 cut(s) 955
CviAII CATG 2 cut(s) 613, 925
CviQI GTAC 1 cut(s) 955
DdeI CTNAG 6 cut(s) 8, 302, 416, 501, 779, 854
DpnI GATC 8 cut(s) 209, 257, 282, 321, 464, 630, 932, 993
DpnII GATC 8 cut(s) 207, 255, 280, 319, 462, 628, 930, 991
DraIII CACNNNGTG 1 cut(s) 752
EaeI YGGCCR 2 cut(s) 198, 324
Eam1104I CTCTTC 2 cut(s) 330, 483
EarI CTCTTC 2 cut(s) 330, 483
EciI GGCGGA 1 cut(s) 446
Ecl136II GAGCTC 1 cut(s) 435
Eco130I CCWWGG 4 cut(s) 612, 632, 696, 880
Eco24I GRGCYC 4 cut(s) 159, 437, 801, 873
Eco47I GGWCC 1 cut(s) 649
Eco47III AGCGCT 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 435
Eco57I CTGAAG 2 cut(s) 354, 425
Eco81I CCTNAGG 1 cut(s) 854
Eco88I CYCGRG 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 435
EcoO109I RGGNCCY 1 cut(s) 693
EcoRI GAATTC 1 cut(s) 985
EcoRII CCWGG 3 cut(s) 117, 203, 689
EcoT14I CCWWGG 4 cut(s) 612, 632, 696, 880
EcoT38I GRGCYC 4 cut(s) 159, 437, 801, 873
ErhI CCWWGG 4 cut(s) 612, 632, 696, 880
FaeI CATG 2 cut(s) 616, 928
FaiI YATR 9 cut(s) 380, 599, 614, 734, 758, 836, 926, 936, 1014
FaqI GGGAC 1 cut(s) 208
FatI CATG 2 cut(s) 612, 924
FauI CCCGC 1 cut(s) 166
Fnu4HI GCNGC 3 cut(s) 114, 498, 914
FokI GGATG 2 cut(s) 800, 847
FriOI GRGCYC 4 cut(s) 159, 437, 801, 873
Fsp4HI GCNGC 3 cut(s) 114, 498, 914
FspBI CTAG 3 cut(s) 20, 83, 656
GlaI GCGC 2 cut(s) 6, 18
GluI GCNGC 3 cut(s) 114, 498, 914
GsaI CCCAGC 1 cut(s) 728
GsuI CTGGAG 1 cut(s) 725
HaeII RGCGCY 2 cut(s) 8, 20
HaeIII GGCC 6 cut(s) 200, 326, 568, 618, 689, 695
HgaI GACGC 2 cut(s) 47, 990
HhaI GCGC 2 cut(s) 7, 19
Hin1I GRCGYC 3 cut(s) 39, 53, 1001
Hin1II CATG 2 cut(s) 616, 928
Hin6I GCGC 2 cut(s) 5, 17
HinP1I GCGC 2 cut(s) 5, 17
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 5 cut(s) 183, 300, 338, 351, 524
HphI GGTGA 3 cut(s) 136, 719, 757
Hpy166II GTNNAC 3 cut(s) 32, 268, 710
Hpy188I TCNGA 8 cut(s) 11, 47, 154, 236, 451, 548, 984, 991
Hpy188III TCNNGA 5 cut(s) 428, 518, 742, 856, 967
Hpy8I GTNNAC 3 cut(s) 32, 268, 710
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 400, 467, 664
HpyCH4III ACNGT 1 cut(s) 719
HpyCH4IV ACGT 2 cut(s) 53, 534
HpyCH4V TGCA 3 cut(s) 113, 581, 703
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 119, 465, 506, 661, 919
HpyF3I CTNAG 6 cut(s) 8, 302, 416, 501, 779, 854
HpySE526I ACGT 2 cut(s) 53, 534
Hsp92I GRCGYC 3 cut(s) 39, 53, 1001
Hsp92II CATG 2 cut(s) 616, 928
HspAI GCGC 2 cut(s) 5, 17
Kpn2I TCCGGA 2 cut(s) 427, 966
Kzo9I GATC 8 cut(s) 207, 255, 280, 319, 462, 628, 930, 991
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 7 cut(s) 154, 249, 291, 440, 775, 796, 969
Lsp1109I GCAGC 3 cut(s) 125, 509, 925
MaeI CTAG 3 cut(s) 20, 83, 656
MaeII ACGT 2 cut(s) 53, 534
MaeIII GTNAC 3 cut(s) 49, 54, 814
MalI GATC 8 cut(s) 209, 257, 282, 321, 464, 630, 932, 993
MboI GATC 8 cut(s) 207, 255, 280, 319, 462, 628, 930, 991
MboII GAAGA 4 cut(s) 178, 347, 500, 880
MflI RGATCY 4 cut(s) 207, 319, 462, 930
MhlI GDGCHC 5 cut(s) 90, 159, 437, 801, 873
MluCI AATT 2 cut(s) 600, 985
MlyI GAGTC 3 cut(s) 294, 347, 533
MmeI TCCRAC 2 cut(s) 214, 916
MnlI CCTC 7 cut(s) 157, 318, 375, 415, 630, 829, 863
MroI TCCGGA 2 cut(s) 427, 966
MseI TTAA 1 cut(s) 903
MspA1I CMGCKG 2 cut(s) 332, 500
MspR9I CCNGG 4 cut(s) 119, 205, 212, 691
MvaI CCWGG 3 cut(s) 119, 205, 691
MwoI GCNNNNNNNGC 6 cut(s) 25, 119, 465, 506, 661, 919
NciI CCSGG 1 cut(s) 212
NcoI CCATGG 1 cut(s) 612
NdeII GATC 8 cut(s) 207, 255, 280, 319, 462, 628, 930, 991
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 2 cut(s) 616, 928
NlaIV GGNNCC 8 cut(s) 89, 156, 209, 287, 321, 426, 754, 798
NmeAIII GCCGAG 2 cut(s) 176, 187
NmuCI GTSAC 3 cut(s) 49, 54, 814
NspV TTCGAA 1 cut(s) 949
PaqCI CACCTGC 1 cut(s) 382
PciSI GCTCTTC 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PfeI GAWTC 2 cut(s) 183, 351
PflFI GACNNNGTC 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 3 cut(s) 115, 499, 915
PleI GAGTC 3 cut(s) 294, 346, 532
PpsI GAGTC 3 cut(s) 294, 346, 532
Psp124BI GAGCTC 1 cut(s) 437
Psp6I CCWGG 3 cut(s) 117, 203, 689
PspFI CCCAGC 1 cut(s) 724
PspGI CCWGG 3 cut(s) 117, 203, 689
PspN4I GGNNCC 8 cut(s) 89, 156, 209, 287, 321, 426, 754, 798
PspPI GGNCC 5 cut(s) 567, 616, 649, 687, 693
PsrI GAACNNNNNNTAC 2 cut(s) 437, 469
PsuI RGATCY 4 cut(s) 207, 319, 462, 930
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 1 cut(s) 956
RsaNI GTAC 1 cut(s) 955
SacI GAGCTC 1 cut(s) 437
SapI GCTCTTC 1 cut(s) 483
SaqAI TTAA 1 cut(s) 903
SatI GCNGC 3 cut(s) 114, 498, 914
Sau3AI GATC 8 cut(s) 207, 255, 280, 319, 462, 628, 930, 991
Sau96I GGNCC 5 cut(s) 567, 616, 649, 687, 693
SchI GAGTC 3 cut(s) 294, 347, 533
ScrFI CCNGG 4 cut(s) 119, 205, 212, 691
SduI GDGCHC 5 cut(s) 90, 159, 437, 801, 873
SfuI TTCGAA 1 cut(s) 949
SinI GGWCC 1 cut(s) 649
Sse9I AATT 2 cut(s) 600, 985
SsiI CCGC 4 cut(s) 159, 314, 330, 457
SspMI CTAG 3 cut(s) 20, 83, 656
SstI GAGCTC 1 cut(s) 437
StyD4I CCNGG 4 cut(s) 117, 203, 210, 689
StyI CCWWGG 4 cut(s) 612, 632, 696, 880
TaaI ACNGT 1 cut(s) 719
TaiI ACGT 2 cut(s) 56, 537
TaqI TCGA 4 cut(s) 36, 519, 949, 1045
TasI AATT 2 cut(s) 600, 985
TatI WGTACW 1 cut(s) 954
TfiI GAWTC 2 cut(s) 183, 351
Tru1I TTAA 1 cut(s) 903
Tru9I TTAA 1 cut(s) 903
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 3 cut(s) 49, 54, 814
TseI GCWGC 3 cut(s) 113, 497, 913
Tsp45I GTSAC 3 cut(s) 49, 54, 814
TspGWI ACGGA 6 cut(s) 61, 236, 291, 346, 363, 427
TspRI CASTG 1 cut(s) 63
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 649
XapI RAATTY 1 cut(s) 985
XspI CTAG 3 cut(s) 20, 83, 656
ZraI GACGTC 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.