MD08G1036100.v1.1

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012447
Physical Location & Seq
Forward (+)
2639240 .. 2639797
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1036100.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGCAGTTCACCAAAACATCGTGCCTCCGAATGCCGCCCAGGTGAATTTCCACAGGGAGTATCTGACCTCGATGGACACCCCCCGGAAAGGATTTTTCCGCCGATTCCTCCACCGCCAGTCGGCATCGAAGCTCCCGGAATTCCTGACCGTTCCGGTTGGGGAGAAGCTCCGGGAAAAGCTGAGAGGCATCAACATTAGCGGTGATCAGCTCCGTCTCGGTGGTCTCAGCCCTCCGCCTCCAGATCGGGACCCCACCGCCAAGCTTCCGTTAGGAATCTCGGTCCCCAACGCGAAGAAGATTCTGAGGCTTTCTCAGGTGGAGAAGCTGAAGGCGAAGCTGAGGGAGATTCCGGAAACTTCCATTTCGCGATCGGAGTTCGTTCGGATCTGCAGCGAAGGATGCGAGAGCGAAGAGCAAGGTGCTGAGTTCGCGAAGATGATGGACGAGTCCGTAAACGTTATCGTTTTGGGAAACGTAGTGTTTCTCCGGCCGGAGCAGGTAACTTCTTCTTCTTTCCCGCTTTTATGTAGATTCTGTTTCTGTTGTTGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04218 ko04621 map04020 map04218 map04621
Pfam Domains
Protein Families
MCU

Protein Analysis

186

Amino Acids

20.75

Weight (kDa)

9.06

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42610
fragaria_vesca FvH4_2g40260
malus_domestica MD00G1134100.v1.1 MD08G1036100.v1.1 MD15G1032500.v1.1
prunus_persica Prupe.1G383500_v2.0.a1
pyrus_communis pycom08g02890 pycom15g02820
rosa_chinensis RchiOBHm_Chr6g0300501
rosa_laevigata RLG00000011332
rosa_multiflora Rmu_co8175312.1_g000001 Rmu_sc0001476.1_g000012
rosa_roxburghii Rroxscaffold_7G00167730
rosa_rugosa Rorug06G0296500
rosa_samantha Rh6AG409100 Rh6BG416400 Rh6CG424000 Rh6DG409600
rosa_wichuraiana Rw6G035800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 490
AccII CGCG 3 cut(s) 293, 370, 434
AccIII TCCGGA 1 cut(s) 352
AciI CCGC 7 cut(s) 36, 100, 115, 201, 236, 258, 521
AclI AACGTT 1 cut(s) 459
AclWI GGATC 1 cut(s) 395
AcoI YGGCCR 1 cut(s) 491
AcsI RAATTY 2 cut(s) 46, 140
AcuI CTGAAG 1 cut(s) 350
AfiI CCNNNNNNNGG 2 cut(s) 89, 121
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 7 cut(s) 133, 169, 181, 211, 265, 328, 340
AluI AGCT 7 cut(s) 133, 169, 181, 211, 265, 328, 340
Alw26I GTCTC 2 cut(s) 221, 230
AlwI GGATC 1 cut(s) 395
Aor13HI TCCGGA 1 cut(s) 352
AoxI GGCC 1 cut(s) 491
ApeKI GCWGC 1 cut(s) 393
ApoI RAATTY 2 cut(s) 46, 140
AspS9I GGNCC 2 cut(s) 250, 283
AsuC2I CCSGG 3 cut(s) 85, 137, 173
AsuHPI GGTGA 2 cut(s) 55, 215
AvaII GGWCC 2 cut(s) 250, 283
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 1 cut(s) 405
BccI CCATC 2 cut(s) 67, 436
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 205
BcnI CCSGG 3 cut(s) 85, 137, 173
BcoDI GTCTC 2 cut(s) 221, 230
BfmI CTRYAG 1 cut(s) 391
BfuAI ACCTGC 1 cut(s) 490
BisI GCNGC 2 cut(s) 36, 394
BlsI GCNGC 2 cut(s) 37, 395
Bme1390I CCNGG 4 cut(s) 41, 85, 137, 173
Bme18I GGWCC 2 cut(s) 250, 283
BmgT120I GGNCC 2 cut(s) 250, 283
BmiI GGNNCC 3 cut(s) 251, 252, 285
BmrFI CCNGG 4 cut(s) 41, 85, 137, 173
BmsI GCATC 3 cut(s) 134, 198, 392
BplI GAGNNNNNCTC 2 cut(s) 298, 330
BpmI CTGGAG 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 341
BpuMI CCSGG 3 cut(s) 85, 137, 173
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 39, 83
BsaWI WCCGGW 2 cut(s) 154, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 121
Bse1I ACTGG 1 cut(s) 118
BseAI TCCGGA 1 cut(s) 352
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 39, 83
BseGI GGATG 1 cut(s) 407
BseLI CCNNNNNNNGG 2 cut(s) 89, 121
BseMII CTCAG 6 cut(s) 173, 241, 296, 329, 332, 417
BseNI ACTGG 1 cut(s) 118
BseX3I CGGCCG 1 cut(s) 491
BseXI GCAGC 1 cut(s) 405
Bsh1236I CGCG 3 cut(s) 293, 370, 434
Bsh1285I CGRYCG 2 cut(s) 374, 494
BshFI GGCC 1 cut(s) 493
BsiEI CGRYCG 2 cut(s) 374, 494
BsiSI CCGG 7 cut(s) 85, 137, 155, 172, 353, 490, 494
BslFI GGGAC 2 cut(s) 263, 269
BslI CCNNNNNNNGG 2 cut(s) 89, 121
BsmAI GTCTC 2 cut(s) 221, 230
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 2 cut(s) 263, 269
BsmI GAATGC 1 cut(s) 37
BsnI GGCC 1 cut(s) 493
Bso31I GGTCTC 1 cut(s) 230
Bsp13I TCCGGA 1 cut(s) 352
Bsp143I GATC 4 cut(s) 205, 244, 371, 387
Bsp68I TCGCGA 2 cut(s) 370, 434
BspACI CCGC 7 cut(s) 36, 100, 115, 201, 236, 258, 521
BspANI GGCC 1 cut(s) 493
BspCNI CTCAG 6 cut(s) 174, 240, 297, 328, 333, 418
BspEI TCCGGA 1 cut(s) 352
BspFNI CGCG 3 cut(s) 293, 370, 434
BspLI GGNNCC 3 cut(s) 251, 252, 285
BspMAI CTGCAG 1 cut(s) 395
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 1 cut(s) 395
BspQI GCTCTTC 1 cut(s) 408
BspTNI GGTCTC 1 cut(s) 230
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 39, 83
BssMI GATC 4 cut(s) 205, 244, 371, 387
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 151
Bst6I CTCTTC 1 cut(s) 408
BstDEI CTNAG 6 cut(s) 182, 227, 305, 315, 341, 426
BstF5I GGATG 1 cut(s) 407
BstFNI CGCG 3 cut(s) 293, 370, 434
BstKTI GATC 4 cut(s) 208, 247, 374, 390
BstMAI GTCTC 2 cut(s) 221, 230
BstMBI GATC 4 cut(s) 205, 244, 371, 387
BstMCI CGRYCG 2 cut(s) 374, 494
BstMWI GCNNNNNNNGC 2 cut(s) 402, 431
BstNI CCWGG 1 cut(s) 41
BstSCI CCNGG 4 cut(s) 39, 83, 135, 171
BstSFI CTRYAG 1 cut(s) 391
BstUI CGCG 3 cut(s) 293, 370, 434
BstV1I GCAGC 1 cut(s) 405
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
BstZI CGGCCG 1 cut(s) 491
BsuRI GGCC 1 cut(s) 493
BtsCI GGATG 1 cut(s) 407
BtuMI TCGCGA 2 cut(s) 370, 434
BveI ACCTGC 1 cut(s) 490
Cfr13I GGNCC 2 cut(s) 250, 283
DdeI CTNAG 6 cut(s) 182, 227, 305, 315, 341, 426
DpnI GATC 4 cut(s) 207, 246, 373, 389
DpnII GATC 4 cut(s) 205, 244, 371, 387
EaeI YGGCCR 1 cut(s) 491
EagI CGGCCG 1 cut(s) 491
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 2 cut(s) 89, 225
EclXI CGGCCG 1 cut(s) 491
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 250, 283
Eco52I CGGCCG 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 250
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 39
Esp3I CGTCTC 1 cut(s) 221
FaiI YATR 1 cut(s) 529
FaqI GGGAC 2 cut(s) 263, 269
FauI CCCGC 1 cut(s) 528
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 2 cut(s) 36, 394
FokI GGATG 1 cut(s) 414
Fsp4HI GCNGC 2 cut(s) 36, 394
GluI GCNGC 2 cut(s) 36, 394
GsuI CTGGAG 1 cut(s) 225
HaeIII GGCC 1 cut(s) 493
HapII CCGG 7 cut(s) 85, 137, 155, 172, 353, 490, 494
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 6 cut(s) 105, 276, 301, 349, 449, 534
HpaII CCGG 7 cut(s) 85, 137, 155, 172, 353, 490, 494
HphI GGTGA 2 cut(s) 55, 215
Hpy166II GTNNAC 2 cut(s) 10, 457
Hpy188I TCNGA 5 cut(s) 30, 66, 306, 376, 387
Hpy188III TCNNGA 6 cut(s) 145, 242, 248, 353, 369, 433
Hpy8I GTNNAC 2 cut(s) 10, 457
HpyAV CCTTC 2 cut(s) 325, 392
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 2 cut(s) 459, 477
HpyCH4V TGCA 1 cut(s) 393
HpyF10VI GCNNNNNNNGC 2 cut(s) 402, 431
HpyF3I CTNAG 6 cut(s) 182, 227, 305, 315, 341, 426
HpySE526I ACGT 2 cut(s) 459, 477
KflI GGGWCCC 1 cut(s) 250
Kpn2I TCCGGA 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 4 cut(s) 205, 244, 371, 387
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 4 cut(s) 138, 174, 216, 496
Lsp1109I GCAGC 1 cut(s) 405
LweI GCATC 3 cut(s) 134, 198, 392
MaeII ACGT 2 cut(s) 459, 477
MaeIII GTNAC 1 cut(s) 502
MalI GATC 4 cut(s) 207, 246, 373, 389
MboI GATC 4 cut(s) 205, 244, 371, 387
MboII GAAGA 6 cut(s) 307, 310, 425, 448, 501, 504
MflI RGATCY 1 cut(s) 387
MluCI AATT 2 cut(s) 46, 140
MlyI GAGTC 1 cut(s) 458
MnlI CCTC 8 cut(s) 36, 79, 119, 179, 243, 249, 300, 336
MroI TCCGGA 1 cut(s) 352
MspI CCGG 7 cut(s) 85, 137, 155, 172, 353, 490, 494
MspR9I CCNGG 4 cut(s) 41, 85, 137, 173
Mva1269I GAATGC 1 cut(s) 37
MvaI CCWGG 1 cut(s) 41
MvnI CGCG 3 cut(s) 293, 370, 434
MwoI GCNNNNNNNGC 2 cut(s) 402, 431
NciI CCSGG 3 cut(s) 85, 137, 173
NdeII GATC 4 cut(s) 205, 244, 371, 387
NlaIV GGNNCC 3 cut(s) 251, 252, 285
NruI TCGCGA 2 cut(s) 370, 434
PciSI GCTCTTC 1 cut(s) 408
PctI GAATGC 1 cut(s) 37
PfeI GAWTC 5 cut(s) 105, 276, 301, 349, 534
PfoI TCCNGGA 2 cut(s) 135, 171
PkrI GCNGC 2 cut(s) 37, 395
Ple19I CGATCG 1 cut(s) 374
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 250
Psp1406I AACGTT 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 3 cut(s) 251, 252, 285
PspPI GGNCC 2 cut(s) 250, 283
PspPPI RGGWCCY 1 cut(s) 250
PstI CTGCAG 1 cut(s) 395
PsuI RGATCY 1 cut(s) 387
PvuI CGATCG 1 cut(s) 374
RruI TCGCGA 2 cut(s) 370, 434
SapI GCTCTTC 1 cut(s) 408
SatI GCNGC 2 cut(s) 36, 394
Sau3AI GATC 4 cut(s) 205, 244, 371, 387
Sau96I GGNCC 2 cut(s) 250, 283
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 4 cut(s) 41, 85, 137, 173
SfaNI GCATC 3 cut(s) 134, 198, 392
SfcI CTRYAG 1 cut(s) 391
SinI GGWCC 2 cut(s) 250, 283
Sse9I AATT 2 cut(s) 46, 140
SsiI CCGC 7 cut(s) 36, 100, 115, 201, 236, 258, 521
StyD4I CCNGG 4 cut(s) 39, 83, 135, 171
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 2 cut(s) 462, 480
TaqI TCGA 2 cut(s) 71, 128
TaqII GACCGA 1 cut(s) 271
TasI AATT 2 cut(s) 46, 140
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 5 cut(s) 105, 276, 301, 349, 534
TseI GCWGC 1 cut(s) 393
TspGWI ACGGA 3 cut(s) 203, 258, 442
VpaK11BI GGWCC 2 cut(s) 250, 283
XapI RAATTY 2 cut(s) 46, 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.