MD15G1032500.v1.1

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012447
Physical Location & Seq
Forward (+)
2016403 .. 2017927
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1032500.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGCGCTCCGTCAAGCGCTTGCTAAGCGCCCATTTGGCGCCACCAGAGTGTCCCTAACGCCACCATCGTCGGTCATTACGGATCACCAAACCATCGTACCCCCGAATGCCGCCCAGGCGAGTTTCCACAGGGAGTATCTGACCTCGACGGACACACCCCGGAAAGGATTTTTCCGGCGATTTCTCCACAGACAGTCGGCAGCCAAGCTCCCGGAATTCCTGACCGTTCCGGTCGGGGAGAAACTCCGGGAAAAGCTGAGAGGCATCAACATTAGCGCTGATCGGCTCCGTCTCGGCGGTATCAACCATCCACCGCCAGATCGGGCCCCCAACGCCGAGCTTCCGTACGGAATCTCGGTCAGCGACGCTAAGAAGATTCTGAGGCTTTCTCAGGTGGAGAAGCTGAAGGCAAAGCTGAGGGAGATTCCTGAGAGTTCCATTTCGCGATCGGAGTTCGTTCGGATCTGCGTCGAAGGCTGCGGGAGCGAAGAGCAAGGTGCTGAGTTCGCGAAGATGATGGACGAGTCCGGAAACGTCATCATTTTGGGAAACGCGGTGTTTCTCCGACCGGAGCAGGTGGCAAAATCAGTGGAGAGCATAATCTCACAAACCATGCCCACCCCGAACGATCCAAGAAGAAAAGAGCTGCAGCAGATGGAAACTCAAAAGATGGTAATTGACCAAAAAGCCAGGGCCCTGGTCCGTGGGGAGCTCTACTGTGGGCTGGGCTTTTTGTTATTTCAAACGATCGGGGCCATGAGGCTCACTTTCTGGGAGCTGAGCTGGGATGTCATGGAGCCCATTTGCTTCTTCGTCTCGTCCATCGACTTCGCCTTGGGCTACGCCTTTTTCCTCCGGACATCGACGGAGCCCACCTTCAAAGGGTTCTTCCACCGTCGCTTCAAGGCCAAGCAACGGCGGCTCATGGAGGCTCATAAGTTCGATGTCGAAAAGTACAACCAGCTCCGAAAAGTGTTTTATCCGAATTCGGATCATCCGGCGTCGTTTCATCACGAAGAAGGGATGGTTATGGGATCATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04218 ko04621 map04020 map04218 map04621
Pfam Domains
Protein Families
MCU

Protein Analysis

348

Amino Acids

39.41

Weight (kDa)

9.6

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 163 - 322 9.6e-51 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42610
fragaria_vesca FvH4_2g40260
malus_domestica MD00G1134100.v1.1 MD08G1036100.v1.1 MD15G1032500.v1.1
prunus_persica Prupe.1G383500_v2.0.a1
pyrus_communis pycom08g02890 pycom15g02820
rosa_chinensis RchiOBHm_Chr6g0300501
rosa_laevigata RLG00000011332
rosa_multiflora Rmu_co8175312.1_g000001 Rmu_sc0001476.1_g000012
rosa_roxburghii Rroxscaffold_7G00167730
rosa_rugosa Rorug06G0296500
rosa_samantha Rh6AG409100 Rh6BG416400 Rh6CG424000 Rh6DG409600
rosa_wichuraiana Rw6G035800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 565
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 38
AccII CGCG 3 cut(s) 445, 509, 554
AccIII TCCGGA 2 cut(s) 527, 855
AciI CCGC 6 cut(s) 111, 297, 314, 480, 554, 919
AclWI GGATC 5 cut(s) 90, 470, 623, 999, 1042
AcsI RAATTY 2 cut(s) 215, 985
AcuI CTGAAG 1 cut(s) 425
AcyI GRCGYC 2 cut(s) 39, 1001
AfaI GTAC 3 cut(s) 99, 347, 956
AfeI AGCGCT 2 cut(s) 18, 277
AfiI CCNNNNNNNGG 3 cut(s) 164, 882, 915
AgsI TTSAA 3 cut(s) 743, 880, 904
AjnI CCWGG 3 cut(s) 114, 689, 696
AleI CACNNNNGTG 1 cut(s) 47
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 2 cut(s) 296, 820
AlwI GGATC 5 cut(s) 90, 470, 623, 999, 1042
Aor13HI TCCGGA 2 cut(s) 527, 855
Aor51HI AGCGCT 2 cut(s) 18, 277
AoxI GGCC 4 cut(s) 324, 693, 753, 906
ApaI GGGCCC 2 cut(s) 328, 697
ApeKI GCWGC 4 cut(s) 200, 477, 646, 649
ApoI RAATTY 2 cut(s) 215, 985
AspLEI GCGC 5 cut(s) 7, 19, 30, 41, 278
AspS9I GGNCC 6 cut(s) 324, 325, 693, 694, 700, 753
AsuC2I CCSGG 3 cut(s) 160, 212, 248
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 700
BaeGI GKGCMC 2 cut(s) 328, 697
BaeI ACNNNNGTAYC 2 cut(s) 81, 114
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 5 cut(s) 328, 697, 714, 801, 873
Bbv12I GWGCWC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 416
BbvI GCAGC 4 cut(s) 212, 464, 633, 661
BccI CCATC 8 cut(s) 73, 101, 315, 511, 649, 664, 830, 1018
BceAI ACGGC 1 cut(s) 932
BciT130I CCWGG 3 cut(s) 116, 691, 698
BcnI CCSGG 3 cut(s) 160, 212, 248
BcoDI GTCTC 2 cut(s) 296, 820
BfmI CTRYAG 1 cut(s) 647
BfoI RGCGCY 5 cut(s) 8, 20, 31, 42, 279
BfuAI ACCTGC 1 cut(s) 565
BglI GCCNNNNNGGC 2 cut(s) 36, 116
BisI GCNGC 6 cut(s) 111, 201, 478, 647, 650, 920
BlpI GCTNAGC 2 cut(s) 24, 779
BlsI GCNGC 6 cut(s) 112, 202, 479, 648, 651, 921
Bme1390I CCNGG 6 cut(s) 116, 160, 212, 248, 691, 698
Bme18I GGWCC 1 cut(s) 700
BmgT120I GGNCC 6 cut(s) 324, 325, 693, 694, 700, 753
BmiI GGNNCC 8 cut(s) 40, 287, 326, 327, 695, 754, 798, 870
BmrFI CCNGG 6 cut(s) 116, 160, 212, 248, 691, 698
BmsI GCATC 1 cut(s) 273
BplI GAGNNNNNCTC 2 cut(s) 373, 405
Bpu10I CCTNAGC 1 cut(s) 416
Bpu1102I GCTNAGC 2 cut(s) 24, 779
BpuMI CCSGG 3 cut(s) 160, 212, 248
BsaHI GRCGYC 2 cut(s) 39, 1001
BsaJI CCNNGG 6 cut(s) 114, 158, 690, 696, 703, 834
BsaWI WCCGGW 4 cut(s) 229, 527, 568, 855
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 882, 915
BseAI TCCGGA 2 cut(s) 527, 855
BseBI CCWGG 3 cut(s) 116, 691, 698
BseDI CCNNGG 6 cut(s) 114, 158, 690, 696, 703, 834
BseGI GGATG 4 cut(s) 307, 793, 994, 1029
BseLI CCNNNNNNNGG 3 cut(s) 164, 882, 915
BseMII CTCAG 7 cut(s) 248, 371, 404, 407, 420, 492, 770
BseSI GKGCMC 2 cut(s) 328, 697
BseXI GCAGC 4 cut(s) 212, 464, 633, 661
BseYI CCCAGC 2 cut(s) 724, 783
Bsh1236I CGCG 3 cut(s) 445, 509, 554
Bsh1285I CGRYCG 4 cut(s) 234, 449, 569, 750
BshFI GGCC 4 cut(s) 326, 695, 755, 908
BshNI GGYRCC 1 cut(s) 38
BsiEI CGRYCG 4 cut(s) 234, 449, 569, 750
BsiHKAI GWGCWC 1 cut(s) 714
BsiSI CCGG 9 cut(s) 160, 175, 212, 230, 247, 528, 569, 856, 998
BsiWI CGTACG 1 cut(s) 345
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 3 cut(s) 164, 882, 915
BsmAI GTCTC 2 cut(s) 296, 820
BsmBI CGTCTC 2 cut(s) 296, 820
BsmFI GGGAC 1 cut(s) 37
BsmI GAATGC 1 cut(s) 112
BsnI GGCC 4 cut(s) 326, 695, 755, 908
Bsp120I GGGCCC 2 cut(s) 324, 693
Bsp1286I GDGCHC 5 cut(s) 328, 697, 714, 801, 873
Bsp13I TCCGGA 2 cut(s) 527, 855
Bsp143I GATC 9 cut(s) 82, 280, 319, 446, 462, 628, 747, 991, 1034
Bsp1720I GCTNAGC 2 cut(s) 24, 779
Bsp68I TCGCGA 2 cut(s) 445, 509
BspACI CCGC 6 cut(s) 111, 297, 314, 480, 554, 919
BspANI GGCC 4 cut(s) 326, 695, 755, 908
BspCNI CTCAG 7 cut(s) 249, 372, 403, 408, 421, 493, 771
BspEI TCCGGA 2 cut(s) 527, 855
BspFNI CGCG 3 cut(s) 445, 509, 554
BspLI GGNNCC 8 cut(s) 40, 287, 326, 327, 695, 754, 798, 870
BspMAI CTGCAG 1 cut(s) 651
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 5 cut(s) 90, 470, 623, 999, 1042
BspQI GCTCTTC 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 38
BssECI CCNNGG 6 cut(s) 114, 158, 690, 696, 703, 834
BssMI GATC 9 cut(s) 82, 280, 319, 446, 462, 628, 747, 991, 1034
BssNI GRCGYC 2 cut(s) 39, 1001
BssT1I CCWWGG 1 cut(s) 834
Bst2UI CCWGG 3 cut(s) 116, 691, 698
Bst4CI ACNGT 4 cut(s) 195, 226, 719, 896
Bst6I CTCTTC 1 cut(s) 483
BstACI GRCGYC 2 cut(s) 39, 1001
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 9 cut(s) 24, 257, 369, 380, 390, 416, 429, 501, 779
BstDSI CCRYGG 1 cut(s) 703
BstF5I GGATG 4 cut(s) 307, 793, 994, 1029
BstFNI CGCG 3 cut(s) 445, 509, 554
BstH2I RGCGCY 5 cut(s) 8, 20, 31, 42, 279
BstHHI GCGC 5 cut(s) 7, 19, 30, 41, 278
BstKTI GATC 9 cut(s) 85, 283, 322, 449, 465, 631, 750, 994, 1037
BstMAI GTCTC 2 cut(s) 296, 820
BstMBI GATC 9 cut(s) 82, 280, 319, 446, 462, 628, 747, 991, 1034
BstMCI CGRYCG 4 cut(s) 234, 449, 569, 750
BstMWI GCNNNNNNNGC 8 cut(s) 25, 36, 116, 332, 474, 483, 506, 919
BstNI CCWGG 3 cut(s) 116, 691, 698
BstSCI CCNGG 6 cut(s) 114, 158, 210, 246, 689, 696
BstSFI CTRYAG 1 cut(s) 647
BstSLI GKGCMC 2 cut(s) 328, 697
BstUI CGCG 3 cut(s) 445, 509, 554
BstV1I GCAGC 4 cut(s) 212, 464, 633, 661
BstX2I RGATCY 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 697
BstYI RGATCY 1 cut(s) 462
BsuRI GGCC 4 cut(s) 326, 695, 755, 908
BtgI CCRYGG 1 cut(s) 703
BtsCI GGATG 4 cut(s) 307, 793, 994, 1029
BtsIMutI CAGTG 1 cut(s) 594
BtuMI TCGCGA 2 cut(s) 445, 509
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 1 cut(s) 21
CfoI GCGC 5 cut(s) 7, 19, 30, 41, 278
Cfr13I GGNCC 6 cut(s) 324, 325, 693, 694, 700, 753
CseI GACGC 3 cut(s) 374, 457, 990
Csp6I GTAC 3 cut(s) 98, 346, 955
CspCI CAANNNNNGTGG 2 cut(s) 570, 605
CviAII CATG 4 cut(s) 613, 757, 793, 925
CviQI GTAC 3 cut(s) 98, 346, 955
DdeI CTNAG 9 cut(s) 24, 257, 369, 380, 390, 416, 429, 501, 779
DinI GGCGCC 1 cut(s) 40
DpnI GATC 9 cut(s) 84, 282, 321, 448, 464, 630, 749, 993, 1036
DpnII GATC 9 cut(s) 82, 280, 319, 446, 462, 628, 747, 991, 1034
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 1 cut(s) 834
Eco24I GRGCYC 5 cut(s) 328, 697, 714, 801, 873
Eco47I GGWCC 1 cut(s) 700
Eco47III AGCGCT 2 cut(s) 18, 277
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 1 cut(s) 425
EcoICRI GAGCTC 1 cut(s) 712
EcoO109I RGGNCCY 3 cut(s) 325, 693, 694
EcoRI GAATTC 2 cut(s) 215, 985
EcoRII CCWGG 3 cut(s) 114, 689, 696
EcoT14I CCWWGG 1 cut(s) 834
EcoT38I GRGCYC 5 cut(s) 328, 697, 714, 801, 873
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 834
Esp3I CGTCTC 2 cut(s) 296, 820
FaeI CATG 4 cut(s) 616, 760, 796, 928
FaiI YATR 7 cut(s) 599, 614, 758, 794, 926, 936, 1031
FaqI GGGAC 1 cut(s) 37
FatI CATG 4 cut(s) 612, 756, 792, 924
FauI CCCGC 1 cut(s) 473
Fnu4HI GCNGC 6 cut(s) 111, 201, 478, 647, 650, 920
FokI GGATG 4 cut(s) 294, 800, 981, 1036
FriOI GRGCYC 5 cut(s) 328, 697, 714, 801, 873
Fsp4HI GCNGC 6 cut(s) 111, 201, 478, 647, 650, 920
GlaI GCGC 5 cut(s) 6, 18, 29, 40, 277
GluI GCNGC 6 cut(s) 111, 201, 478, 647, 650, 920
GsaI CCCAGC 2 cut(s) 728, 787
HaeII RGCGCY 5 cut(s) 8, 20, 31, 42, 279
HaeIII GGCC 4 cut(s) 326, 695, 755, 908
HapII CCGG 9 cut(s) 160, 175, 212, 230, 247, 528, 569, 856, 998
HgaI GACGC 3 cut(s) 374, 457, 990
HhaI GCGC 5 cut(s) 7, 19, 30, 41, 278
Hin1I GRCGYC 2 cut(s) 39, 1001
Hin1II CATG 4 cut(s) 616, 760, 796, 928
Hin6I GCGC 5 cut(s) 5, 17, 28, 39, 276
HinP1I GCGC 5 cut(s) 5, 17, 28, 39, 276
HinfI GANTC 4 cut(s) 351, 376, 424, 524
HpaII CCGG 9 cut(s) 160, 175, 212, 230, 247, 528, 569, 856, 998
HphI GGTGA 1 cut(s) 77
Hpy188I TCNGA 8 cut(s) 141, 381, 451, 462, 566, 968, 984, 991
Hpy188III TCNNGA 7 cut(s) 220, 428, 444, 508, 528, 856, 1013
Hpy99I CGWCG 7 cut(s) 73, 151, 368, 473, 868, 900, 1006
HpyAV CCTTC 4 cut(s) 400, 467, 886, 1013
HpyCH4III ACNGT 4 cut(s) 195, 226, 719, 896
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 1 cut(s) 649
HpyF10VI GCNNNNNNNGC 8 cut(s) 25, 36, 116, 332, 474, 483, 506, 919
HpyF3I CTNAG 9 cut(s) 24, 257, 369, 380, 390, 416, 429, 501, 779
HpySE526I ACGT 1 cut(s) 534
Hsp92I GRCGYC 2 cut(s) 39, 1001
Hsp92II CATG 4 cut(s) 616, 760, 796, 928
HspAI GCGC 5 cut(s) 5, 17, 28, 39, 276
KasI GGCGCC 1 cut(s) 38
Kpn2I TCCGGA 2 cut(s) 527, 855
Kzo9I GATC 9 cut(s) 82, 280, 319, 446, 462, 628, 747, 991, 1034
LguI GCTCTTC 1 cut(s) 483
Lsp1109I GCAGC 4 cut(s) 212, 464, 633, 661
LweI GCATC 1 cut(s) 273
MaeII ACGT 1 cut(s) 534
MalI GATC 9 cut(s) 84, 282, 321, 448, 464, 630, 749, 993, 1036
MboI GATC 9 cut(s) 82, 280, 319, 446, 462, 628, 747, 991, 1034
MboII GAAGA 7 cut(s) 385, 500, 523, 648, 802, 880, 1028
MflI RGATCY 1 cut(s) 462
MhlI GDGCHC 5 cut(s) 328, 697, 714, 801, 873
MluCI AATT 3 cut(s) 215, 675, 985
Mly113I GGCGCC 1 cut(s) 39
MlyI GAGTC 1 cut(s) 533
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 7 cut(s) 154, 254, 375, 411, 753, 863, 922
MroI TCCGGA 2 cut(s) 527, 855
MslI CAYNNNNRTG 1 cut(s) 47
MspI CCGG 9 cut(s) 160, 175, 212, 230, 247, 528, 569, 856, 998
MspR9I CCNGG 6 cut(s) 116, 160, 212, 248, 691, 698
Mva1269I GAATGC 1 cut(s) 112
MvaI CCWGG 3 cut(s) 116, 691, 698
MvnI CGCG 3 cut(s) 445, 509, 554
MwoI GCNNNNNNNGC 8 cut(s) 25, 36, 116, 332, 474, 483, 506, 919
NarI GGCGCC 1 cut(s) 39
NciI CCSGG 3 cut(s) 160, 212, 248
NdeII GATC 9 cut(s) 82, 280, 319, 446, 462, 628, 747, 991, 1034
NlaIII CATG 4 cut(s) 616, 760, 796, 928
NlaIV GGNNCC 8 cut(s) 40, 287, 326, 327, 695, 754, 798, 870
NmeAIII GCCGAG 2 cut(s) 273, 361
NruI TCGCGA 2 cut(s) 445, 509
OliI CACNNNNGTG 1 cut(s) 47
PaqCI CACCTGC 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PctI GAATGC 1 cut(s) 112
PfeI GAWTC 3 cut(s) 351, 376, 424
Pfl23II CGTACG 1 cut(s) 345
PfoI TCCNGGA 2 cut(s) 210, 246
PkrI GCNGC 6 cut(s) 112, 202, 479, 648, 651, 921
Ple19I CGATCG 2 cut(s) 449, 750
PleI GAGTC 1 cut(s) 532
PluTI GGCGCC 1 cut(s) 42
PpsI GAGTC 1 cut(s) 532
Psp124BI GAGCTC 1 cut(s) 714
Psp6I CCWGG 3 cut(s) 114, 689, 696
PspFI CCCAGC 2 cut(s) 724, 783
PspGI CCWGG 3 cut(s) 114, 689, 696
PspLI CGTACG 1 cut(s) 345
PspN4I GGNNCC 8 cut(s) 40, 287, 326, 327, 695, 754, 798, 870
PspOMI GGGCCC 2 cut(s) 324, 693
PspPI GGNCC 6 cut(s) 324, 325, 693, 694, 700, 753
PstI CTGCAG 1 cut(s) 651
PsuI RGATCY 1 cut(s) 462
PvuI CGATCG 2 cut(s) 449, 750
RruI TCGCGA 2 cut(s) 445, 509
RsaI GTAC 3 cut(s) 99, 347, 956
RsaNI GTAC 3 cut(s) 98, 346, 955
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 483
SatI GCNGC 6 cut(s) 111, 201, 478, 647, 650, 920
Sau3AI GATC 9 cut(s) 82, 280, 319, 446, 462, 628, 747, 991, 1034
Sau96I GGNCC 6 cut(s) 324, 325, 693, 694, 700, 753
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 6 cut(s) 116, 160, 212, 248, 691, 698
SduI GDGCHC 5 cut(s) 328, 697, 714, 801, 873
SfaNI GCATC 1 cut(s) 273
SfcI CTRYAG 1 cut(s) 647
SfoI GGCGCC 1 cut(s) 40
SinI GGWCC 1 cut(s) 700
SmiMI CAYNNNNRTG 1 cut(s) 47
Sse9I AATT 3 cut(s) 215, 675, 985
SsiI CCGC 6 cut(s) 111, 297, 314, 480, 554, 919
SspDI GGCGCC 1 cut(s) 38
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 6 cut(s) 114, 158, 210, 246, 689, 696
StyI CCWWGG 1 cut(s) 834
TaaI ACNGT 4 cut(s) 195, 226, 719, 896
TaiI ACGT 1 cut(s) 537
TaqI TCGA 6 cut(s) 146, 471, 825, 863, 942, 948
TaqII GACCGA 2 cut(s) 61, 346
TasI AATT 3 cut(s) 215, 675, 985
TatI WGTACW 1 cut(s) 954
TauI GCSGC 2 cut(s) 113, 922
TfiI GAWTC 3 cut(s) 351, 376, 424
TscAI CASTG 1 cut(s) 594
TseI GCWGC 4 cut(s) 200, 477, 646, 649
TspDTI ATGAA 1 cut(s) 998
TspGWI ACGGA 7 cut(s) 95, 164, 278, 333, 363, 692, 881
TspRI CASTG 1 cut(s) 594
VpaK11BI GGWCC 1 cut(s) 700
XapI RAATTY 2 cut(s) 215, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.