FvH4_2g41030

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017257
Physical Location & Seq
Reverse (-)
29116588 .. 29119084
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g41030.t2

Sequence Viewer

Length: 1281 bp
ATGTATATATACATCACCTCGCTCGCTCTCTATGCTCATACACCCTTCAAACTTTATTCGCTGCAGATCAGCGAGAAACCAAGTAAACAGAAAGACGGTCTTCATTTTCCTCTATTCAGAGATTCAGAGACTACGACATCAATGGCAAAGAAAAGCTCCAAGGCTAAGATCGCTAAGTGGGGGATACTCTCAAAAAAACAAGAGAATAAACAGATACACAAAGTGGCCTGTGGAAGTTCAAAGAAGGCAGCTCGAAGGCCGGAACGATTAAAGCCATCCCCATCTCAGAAATCATTACTTCTGGAAAAAGCAAATGACATTGCCGATCAACTGCCATTGAAATATCCTAATTTTGTAAAGGTTATGCTTCCCTCCCATGTCTCTGGAGGCTTCTGGCTGGGTCTTCCAGCCAGATTCTGCCAAAAACATATGGAGTCGAGCGACCAGGATATTACCTTAGAAGATGAAGATGGAGAAAAATTCACAACCAAGTATCTCGCTGGAAAGATGGGGCTGAGTGCTGGATGGAGAGGGTTTTCCTTGAAACACCGTCTGGTTCAGGGATACGTATTAGTCTTTCACAAAGTGGAGACTCAAAAGTTCAAGGTTTATATATGTAAACCAAACACTACAAAACAGGTGAAGTTCGCTGCTGATGTATCTCAAACGGCAGTAAACGACACTCAAATGGCGGTCGACACTCAAACCACAGCCTTCAATAGTCCAACGTCATCGGAACTTTCTGAAGATTTATTGTACATTATGGGAGATCATGATGAGGGCAGTACAGAGGGTGGAGATGTATCCAGTTATATTGACGGTTTTGAAGATAACAGTGATTGTGTTCTTTTGGAAAGACCTGGGCTTCCGGAGTCTGCAGCCACTCCTTTCAAAGAAGTGACATGCTTTGATACGTTTAAGGTGATGTTCAATGGCCGGAACATTAATTCCCAGATTCCCAAAGTCGTCTTGAAGAAGTATTATGAACTCTGCAGCAGTCAAAGCTCGCACCTGCATCAACATGTTGTGGAGTCTCTTAACAGCCATCTGGTTGTTGGACTGATAACTGAGACGGTTAACATTGCAGATGCAGTTAAGTCGTGCAAGATTACTACCAAGGAGTGTGAGTTTCAGACATGGGAAAAGACCTTGGAAGCCTCTGAGATCTTAGGCATGAATGTCGGCTTCCTGCGCATCCGAGTGAGGAGGCTTGCGAGTCTTGCAGCAGAGTCACTGCAGGTTGCTGCAAGTTTCAAAAAAGTGACAAATGTGCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

427

Amino Acids

47.66

Weight (kDa)

8.86

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 71 - 161 9.5e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017257)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41030 FvH4_2g41030
rosa_chinensis RchiOBHm_Chr6g0299531
rosa_laevigata RLG00000011418
rosa_multiflora Rmu_sc0004064.1_g000007
rosa_roxburghii Rroxscaffold_7G00168580
rosa_samantha Rh6AG400900 Rh6AG401000 Rh6BG409500 Rh6CG414900 Rh6DG402000 Rh6DG402100
rosa_wichuraiana Rw6G035090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1020
Acc16I TGCGCA 1 cut(s) 1193
Acc36I ACCTGC 2 cut(s) 1020, 1228
AccI GTMKAC 1 cut(s) 696
AccIII TCCGGA 1 cut(s) 868
AciI CCGC 1 cut(s) 692
AcoI YGGCCR 1 cut(s) 934
AcsI RAATTY 1 cut(s) 479
AcuI CTGAAG 1 cut(s) 765
AdeI CACNNNGTG 2 cut(s) 223, 586
AfaI GTAC 2 cut(s) 758, 787
AflIII ACRYGT 1 cut(s) 1021
AjnI CCWGG 2 cut(s) 444, 859
AloI GAACNNNNNNTCC 2 cut(s) 932, 964
AluBI AGCT 3 cut(s) 156, 251, 1005
AluI AGCT 3 cut(s) 156, 251, 1005
Alw26I GTCTC 5 cut(s) 122, 385, 584, 1038, 1064
AlwNI CAGNNNCTG 1 cut(s) 417
Aor13HI TCCGGA 1 cut(s) 868
AoxI GGCC 3 cut(s) 225, 257, 934
ApeKI GCWGC 7 cut(s) 61, 248, 650, 878, 993, 1223, 1244
ApoI RAATTY 1 cut(s) 479
AseI ATTAAT 1 cut(s) 945
AspLEI GCGC 1 cut(s) 1194
AsuHPI GGTGA 3 cut(s) 7, 652, 934
BbsI GAAGAC 2 cut(s) 92, 395
BbvI GCAGC 7 cut(s) 48, 260, 637, 890, 1005, 1231, 1235
BccI CCATC 6 cut(s) 283, 289, 464, 502, 519, 1053
BceAI ACGGC 1 cut(s) 684
BcgI CGANNNNNNTGC 4 cut(s) 1080, 1114, 1162, 1196
BciT130I CCWGG 2 cut(s) 446, 861
BciVI GTATCC 3 cut(s) 177, 557, 814
BcoDI GTCTC 5 cut(s) 122, 385, 584, 1038, 1064
BfmI CTRYAG 4 cut(s) 62, 876, 991, 1235
BfuAI ACCTGC 2 cut(s) 1020, 1228
BfuI GTATCC 3 cut(s) 177, 557, 814
BglII AGATCT 1 cut(s) 1164
BisI GCNGC 7 cut(s) 62, 249, 651, 879, 994, 1224, 1245
BlsI GCNGC 7 cut(s) 63, 250, 652, 880, 995, 1225, 1246
Bme1390I CCNGG 2 cut(s) 446, 861
BmrFI CCNGG 2 cut(s) 446, 861
BmsI GCATC 3 cut(s) 1024, 1078, 1203
BpiI GAAGAC 2 cut(s) 92, 395
BpmI CTGGAG 1 cut(s) 405
BsaAI YACGTR 1 cut(s) 568
BsaJI CCNNGG 5 cut(s) 159, 860, 1116, 1149, 1274
BsaWI WCCGGW 1 cut(s) 868
BsaXI ACNNNNNCTCC 2 cut(s) 1112, 1142
Bse1I ACTGG 1 cut(s) 807
Bse3DI GCAATG 2 cut(s) 318, 1080
BseAI TCCGGA 1 cut(s) 868
BseBI CCWGG 2 cut(s) 446, 861
BseDI CCNNGG 5 cut(s) 159, 860, 1116, 1149, 1274
BseGI GGATG 3 cut(s) 275, 530, 1194
BseMI GCAATG 2 cut(s) 318, 1080
BseMII CTCAG 4 cut(s) 299, 506, 1059, 1152
BseNI ACTGG 1 cut(s) 807
BseRI GAGGAG 1 cut(s) 1219
BseXI GCAGC 7 cut(s) 48, 260, 637, 890, 1005, 1231, 1235
BseYI CCCAGC 1 cut(s) 397
Bsh1285I CGRYCG 1 cut(s) 696
BshFI GGCC 3 cut(s) 227, 259, 936
BsiEI CGRYCG 1 cut(s) 696
BsiSI CCGG 3 cut(s) 260, 869, 937
BsmAI GTCTC 5 cut(s) 122, 385, 584, 1038, 1064
BsmBI CGTCTC 1 cut(s) 1064
BsnI GGCC 3 cut(s) 227, 259, 936
Bsp13I TCCGGA 1 cut(s) 868
Bsp1407I TGTACA 1 cut(s) 756
Bsp143I GATC 5 cut(s) 66, 168, 325, 769, 1164
BspACI CCGC 1 cut(s) 692
BspANI GGCC 3 cut(s) 227, 259, 936
BspCNI CTCAG 4 cut(s) 298, 507, 1060, 1153
BspEI TCCGGA 1 cut(s) 868
BspHI TCATGA 1 cut(s) 772
BspMAI CTGCAG 4 cut(s) 66, 880, 995, 1239
BspMI ACCTGC 2 cut(s) 1020, 1228
BsrDI GCAATG 2 cut(s) 318, 1080
BsrGI TGTACA 1 cut(s) 756
BsrI ACTGG 1 cut(s) 807
BssECI CCNNGG 5 cut(s) 159, 860, 1116, 1149, 1274
BssMI GATC 5 cut(s) 66, 168, 325, 769, 1164
BssT1I CCWWGG 4 cut(s) 159, 1116, 1149, 1274
Bst2UI CCWGG 2 cut(s) 446, 861
Bst4CI ACNGT 5 cut(s) 98, 551, 821, 836, 1075
BstAUI TGTACA 1 cut(s) 756
BstBAI YACGTR 1 cut(s) 568
BstC8I GCNNGC 3 cut(s) 24, 1007, 1212
BstDEI CTNAG 8 cut(s) 165, 174, 285, 457, 515, 1068, 1161, 1168
BstF5I GGATG 3 cut(s) 275, 530, 1194
BstHHI GCGC 1 cut(s) 1194
BstKTI GATC 5 cut(s) 69, 171, 328, 772, 1167
BstMAI GTCTC 5 cut(s) 122, 385, 584, 1038, 1064
BstMBI GATC 5 cut(s) 66, 168, 325, 769, 1164
BstMCI CGRYCG 1 cut(s) 696
BstMWI GCNNNNNNNGC 5 cut(s) 32, 170, 1002, 1191, 1220
BstNI CCWGG 2 cut(s) 446, 861
BstNSI RCATGY 2 cut(s) 906, 1025
BstSCI CCNGG 2 cut(s) 444, 859
BstSFI CTRYAG 4 cut(s) 62, 876, 991, 1235
BstSNI TACGTA 1 cut(s) 568
BstV1I GCAGC 7 cut(s) 48, 260, 637, 890, 1005, 1231, 1235
BstV2I GAAGAC 2 cut(s) 92, 395
BstX2I RGATCY 1 cut(s) 1164
BstXI CCANNNNNNTGG 1 cut(s) 383
BstYI RGATCY 1 cut(s) 1164
BsuI GTATCC 3 cut(s) 177, 557, 814
BsuRI GGCC 3 cut(s) 227, 259, 936
BtsCI GGATG 3 cut(s) 275, 530, 1194
BtsI GCAGTG 1 cut(s) 1232
BtsIMutI CAGTG 2 cut(s) 841, 1232
BveI ACCTGC 2 cut(s) 1020, 1228
Cac8I GCNNGC 3 cut(s) 24, 1007, 1212
CaiI CAGNNNCTG 1 cut(s) 417
CciI TCATGA 1 cut(s) 772
CfoI GCGC 1 cut(s) 1194
Csp6I GTAC 2 cut(s) 757, 786
CviAII CATG 6 cut(s) 377, 773, 903, 1022, 1137, 1174
CviQI GTAC 2 cut(s) 757, 786
DdeI CTNAG 8 cut(s) 165, 174, 285, 457, 515, 1068, 1161, 1168
DpnI GATC 5 cut(s) 68, 170, 327, 771, 1166
DpnII GATC 5 cut(s) 66, 168, 325, 769, 1164
DraIII CACNNNGTG 2 cut(s) 223, 586
EaeI YGGCCR 1 cut(s) 934
Eco105I TACGTA 1 cut(s) 568
Eco130I CCWWGG 4 cut(s) 159, 1116, 1149, 1274
Eco57I CTGAAG 1 cut(s) 765
EcoRII CCWGG 2 cut(s) 444, 859
EcoT14I CCWWGG 4 cut(s) 159, 1116, 1149, 1274
ErhI CCWWGG 4 cut(s) 159, 1116, 1149, 1274
Esp3I CGTCTC 1 cut(s) 1064
FaeI CATG 6 cut(s) 380, 776, 906, 1025, 1140, 1177
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FatI CATG 6 cut(s) 376, 772, 902, 1021, 1136, 1173
FauNDI CATATG 1 cut(s) 429
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 7 cut(s) 62, 249, 651, 879, 994, 1224, 1245
FokI GGATG 3 cut(s) 262, 537, 1181
Fsp4HI GCNGC 7 cut(s) 62, 249, 651, 879, 994, 1224, 1245
FspI TGCGCA 1 cut(s) 1193
GlaI GCGC 1 cut(s) 1193
GluI GCNGC 7 cut(s) 62, 249, 651, 879, 994, 1224, 1245
GsaI CCCAGC 1 cut(s) 401
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 3 cut(s) 227, 259, 936
HapII CCGG 3 cut(s) 260, 869, 937
HhaI GCGC 1 cut(s) 1194
Hin1II CATG 6 cut(s) 380, 776, 906, 1025, 1140, 1177
Hin6I GCGC 1 cut(s) 1192
HinP1I GCGC 1 cut(s) 1192
HincII GTYRAC 2 cut(s) 697, 1078
HindII GTYRAC 2 cut(s) 697, 1078
HinfI GANTC 9 cut(s) 122, 414, 434, 592, 872, 955, 1031, 1216, 1229
HpaI GTTAAC 1 cut(s) 1078
HpaII CCGG 3 cut(s) 260, 869, 937
HphI GGTGA 3 cut(s) 7, 652, 934
Hpy166II GTNNAC 5 cut(s) 86, 620, 676, 697, 1078
Hpy188I TCNGA 8 cut(s) 119, 127, 288, 736, 745, 1134, 1162, 1199
Hpy188III TCNNGA 5 cut(s) 302, 384, 773, 869, 970
Hpy8I GTNNAC 5 cut(s) 86, 620, 676, 697, 1078
HpyAV CCTTC 4 cut(s) 55, 238, 249, 724
HpyCH4III ACNGT 5 cut(s) 98, 551, 821, 836, 1075
HpyCH4IV ACGT 3 cut(s) 567, 728, 914
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 170, 1002, 1191, 1220
HpyF3I CTNAG 8 cut(s) 165, 174, 285, 457, 515, 1068, 1161, 1168
HpySE526I ACGT 3 cut(s) 567, 728, 914
Hsp92II CATG 6 cut(s) 380, 776, 906, 1025, 1140, 1177
HspAI GCGC 1 cut(s) 1192
Kpn2I TCCGGA 1 cut(s) 868
KspAI GTTAAC 1 cut(s) 1078
Kzo9I GATC 5 cut(s) 66, 168, 325, 769, 1164
LmnI GCTCC 1 cut(s) 161
Lsp1109I GCAGC 7 cut(s) 48, 260, 637, 890, 1005, 1231, 1235
LweI GCATC 3 cut(s) 1024, 1078, 1203
MaeII ACGT 3 cut(s) 567, 728, 914
MaeIII GTNAC 3 cut(s) 898, 1230, 1261
MalI GATC 5 cut(s) 68, 170, 327, 771, 1166
MboI GATC 5 cut(s) 66, 168, 325, 769, 1164
MboII GAAGA 7 cut(s) 92, 395, 473, 479, 758, 839, 985
MflI RGATCY 1 cut(s) 1164
MluCI AATT 3 cut(s) 349, 479, 946
MlyI GAGTC 6 cut(s) 443, 586, 881, 1040, 1225, 1238
MmeI TCCRAC 2 cut(s) 749, 1036
MroI TCCGGA 1 cut(s) 868
MseI TTAA 6 cut(s) 269, 918, 945, 1038, 1077, 1095
MslI CAYNNNNRTG 3 cut(s) 686, 1020, 1199
MspI CCGG 3 cut(s) 260, 869, 937
MspR9I CCNGG 2 cut(s) 446, 861
MvaI CCWGG 2 cut(s) 446, 861
MwoI GCNNNNNNNGC 5 cut(s) 32, 170, 1002, 1191, 1220
NdeI CATATG 1 cut(s) 429
NdeII GATC 5 cut(s) 66, 168, 325, 769, 1164
NlaIII CATG 6 cut(s) 380, 776, 906, 1025, 1140, 1177
NmuCI GTSAC 3 cut(s) 898, 1230, 1261
NsbI TGCGCA 1 cut(s) 1193
NspI RCATGY 2 cut(s) 906, 1025
PagI TCATGA 1 cut(s) 772
PaqCI CACCTGC 1 cut(s) 1020
PciI ACATGT 1 cut(s) 1021
PfeI GAWTC 3 cut(s) 122, 414, 955
PkrI GCNGC 7 cut(s) 63, 250, 652, 880, 995, 1225, 1246
PleI GAGTC 6 cut(s) 442, 586, 880, 1039, 1224, 1237
PpsI GAGTC 6 cut(s) 442, 586, 880, 1039, 1224, 1237
Ppu21I YACGTR 1 cut(s) 568
PscI ACATGT 1 cut(s) 1021
PshBI ATTAAT 1 cut(s) 945
Psp6I CCWGG 2 cut(s) 444, 859
PspFI CCCAGC 1 cut(s) 397
PspGI CCWGG 2 cut(s) 444, 859
PstI CTGCAG 4 cut(s) 66, 880, 995, 1239
PstNI CAGNNNCTG 1 cut(s) 417
PsuI RGATCY 1 cut(s) 1164
RsaI GTAC 2 cut(s) 758, 787
RsaNI GTAC 2 cut(s) 757, 786
RseI CAYNNNNRTG 3 cut(s) 686, 1020, 1199
SalI GTCGAC 1 cut(s) 695
SaqAI TTAA 6 cut(s) 269, 918, 945, 1038, 1077, 1095
SatI GCNGC 7 cut(s) 62, 249, 651, 879, 994, 1224, 1245
Sau3AI GATC 5 cut(s) 66, 168, 325, 769, 1164
SchI GAGTC 6 cut(s) 443, 586, 881, 1040, 1225, 1238
ScrFI CCNGG 2 cut(s) 446, 861
SfaNI GCATC 3 cut(s) 1024, 1078, 1203
SfcI CTRYAG 4 cut(s) 62, 876, 991, 1235
SmiMI CAYNNNNRTG 3 cut(s) 686, 1020, 1199
SnaBI TACGTA 1 cut(s) 568
Sse9I AATT 3 cut(s) 349, 479, 946
SsiI CCGC 1 cut(s) 692
StyD4I CCNGG 2 cut(s) 444, 859
StyI CCWWGG 4 cut(s) 159, 1116, 1149, 1274
TaaI ACNGT 5 cut(s) 98, 551, 821, 836, 1075
TaiI ACGT 3 cut(s) 570, 731, 917
TaqI TCGA 3 cut(s) 253, 437, 696
TasI AATT 3 cut(s) 349, 479, 946
TatI WGTACW 2 cut(s) 756, 785
TfiI GAWTC 3 cut(s) 122, 414, 955
Tru1I TTAA 6 cut(s) 269, 918, 945, 1038, 1077, 1095
Tru9I TTAA 6 cut(s) 269, 918, 945, 1038, 1077, 1095
TscAI CASTG 2 cut(s) 841, 1239
TseFI GTSAC 3 cut(s) 898, 1230, 1261
TseI GCWGC 7 cut(s) 61, 248, 650, 878, 993, 1223, 1244
Tsp45I GTSAC 3 cut(s) 898, 1230, 1261
TspDTI ATGAA 4 cut(s) 92, 480, 999, 1190
TspRI CASTG 2 cut(s) 841, 1239
VspI ATTAAT 1 cut(s) 945
XapI RAATTY 1 cut(s) 479
XceI RCATGY 2 cut(s) 906, 1025
XcmI CCANNNNNNNNNTGG 1 cut(s) 1052
XmiI GTMKAC 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.