Rh6BG409500

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0017257
Physical Location & Seq
Forward (+)
63536978 .. 63539705
2728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG409500.1

Sequence Viewer

Length: 1158 bp
ATGCCAATTGATGATTCTAAATTTTCTCTAAAAAACAAGAAGGTTAAATCAAATGAAAGACTCATTATATTTATCATTAAGGAACGTGACACGATTAGATTATGGCGTCAAGTACGTTTGACACGACGATCACTACCACCTTCGAAGGCGCCCCCAATTGAGGGTCTTCCTTCGACATTCTGCCACAAACATATAGGAACAAGCGACCAGACGATTATATTGGAAGATGAAACTGGCGATAAGTTCAAAACAACATATCTCGGTCATAAGATGGGACTAAGTGCTGGATGGAGAGGGTTTTCCATTCATCACCATTTGCTTGAGGGAGATGTTTTAGTCTTCCAAAAAGTCAGGGCTCATGAATTTAAGGTTTATATAGTTAAACCAAATGGTTCAAAAAAACTGAAGTTTCCCGGCGATCACGCAGAGGACTCTCAAATGGCATCCGTTACTACATGTAGTATTCCAACAACATGTGATCTTTCTGAAGATATATTGTACATTGACAATTATGTCGGAGGTACAGAGAGTGAAGAAGATATGTTCAATTATATTGACAATTATGAAGCCGGTGCAGAGAGTGAAGATGATATTGACCCTGAAATTTTCAAAGGAGCTGGGCTTTCGGAGTCTTCTGTCACTTGTTTTAAAGAAGTGACATGCATCGAGAAGTTCAAGATCATATTCAATGGCTGTAACATCAATTCCCAGGTTCCTAAACTCGTCCTGAAGAAATATTATGAGCTTTGCCGCGGCCAAAACTCATACCTGCATGAAAATCTTGTGGAGTCTCTTAACAGCCAGCTGGTTGCTGGACTAATCTCTGAGACGGTCAATATTGCAGATGCAATTAAATTCTGCAAGATTACCACCGAGGAGTCTGAGTTTTCGAAGTGGGACAAGACCTTGCAAGCCTCTGAGATGTTAGGCATGAATGTGGGCTTCATGCGCAGCCGAGCGAGCAAGCTTGCAAGCCTTGCGGCAGAATCAAAGACGTACAAAGATGCGAGACTTGCAAGAGAGCAAGAAGAAGCAGAGTTTCGGGTCAGGTTGGCTGACTTTTTGAAAGAAAAAGATAAAGCAGATCGGGAGGGGTCTGAGAAATCAGTGCAGGTTGCTGCAAGTTTCAAACAAGTACTGACAAATGTACCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

385

Amino Acids

43.55

Weight (kDa)

6.06

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 56 - 127 8.1e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017257)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41030 FvH4_2g41030
rosa_chinensis RchiOBHm_Chr6g0299531
rosa_laevigata RLG00000011418
rosa_multiflora Rmu_sc0004064.1_g000007
rosa_roxburghii Rroxscaffold_7G00168580
rosa_samantha Rh6AG400900 Rh6AG401000 Rh6BG409500 Rh6CG414900 Rh6DG402000 Rh6DG402100
rosa_wichuraiana Rw6G035090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 512
Acc16I TGCGCA 1 cut(s) 950
Acc36I ACCTGC 2 cut(s) 777, 1102
AccB1I GGYRCC 1 cut(s) 148
AccII CGCG 1 cut(s) 753
AciI CCGC 3 cut(s) 751, 753, 980
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 4 cut(s) 20, 362, 603, 854
AcuI CTGAAG 3 cut(s) 425, 507, 749
AcyI GRCGYC 2 cut(s) 106, 149
AfaI GTAC 6 cut(s) 114, 500, 523, 998, 1137, 1149
AfiI CCNNNNNNNGG 2 cut(s) 160, 161
AflIII ACRYGT 2 cut(s) 455, 473
AgsI TTSAA 8 cut(s) 247, 396, 547, 610, 676, 688, 1066, 1129
AjnI CCWGG 1 cut(s) 708
AjuI GAANNNNNNNTTGG 1 cut(s) 30
AluBI AGCT 4 cut(s) 617, 745, 805, 967
AluI AGCT 4 cut(s) 617, 745, 805, 967
Alw26I GTCTC 3 cut(s) 795, 821, 1003
AoxI GGCC 1 cut(s) 754
ApeKI GCWGC 2 cut(s) 951, 1118
ApoI RAATTY 4 cut(s) 20, 362, 603, 854
AspLEI GCGC 2 cut(s) 151, 951
AsuC2I CCSGG 1 cut(s) 414
AsuHPI GGTGA 1 cut(s) 302
AsuII TTCGAA 2 cut(s) 143, 890
BaeI ACNNNNGTAYC 1 cut(s) 1131
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 358
BbsI GAAGAC 3 cut(s) 158, 331, 624
BbvI GCAGC 2 cut(s) 963, 1105
BccI CCATC 2 cut(s) 265, 282
BciT130I CCWGG 1 cut(s) 710
BcnI CCSGG 1 cut(s) 414
BcoDI GTCTC 3 cut(s) 795, 821, 1003
BfoI RGCGCY 1 cut(s) 152
BfuAI ACCTGC 2 cut(s) 777, 1102
BisI GCNGC 5 cut(s) 751, 754, 952, 981, 1119
BlsI GCNGC 5 cut(s) 752, 755, 953, 982, 1120
BmcAI AGTACT 1 cut(s) 1137
Bme1390I CCNGG 2 cut(s) 414, 710
BmiI GGNNCC 2 cut(s) 150, 714
BmrFI CCNGG 2 cut(s) 414, 710
BmsI GCATC 4 cut(s) 452, 672, 835, 994
BpiI GAAGAC 3 cut(s) 158, 331, 624
Bpu14I TTCGAA 2 cut(s) 143, 890
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 414
BsaHI GRCGYC 2 cut(s) 106, 149
BsaJI CCNNGG 4 cut(s) 708, 751, 873, 1151
BsaXI ACNNNNNCTCC 2 cut(s) 869, 899
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 161
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 1 cut(s) 238
BseBI CCWGG 1 cut(s) 710
BseDI CCNNGG 4 cut(s) 708, 751, 873, 1151
BseGI GGATG 2 cut(s) 293, 443
BseLI CCNNNNNNNGG 2 cut(s) 160, 161
BseMII CTCAG 4 cut(s) 816, 873, 909, 1089
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 1 cut(s) 890
BseXI GCAGC 2 cut(s) 963, 1105
BseYI CCCAGC 1 cut(s) 617
BsgI GTGCAG 2 cut(s) 594, 1130
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 1 cut(s) 756
BshNI GGYRCC 1 cut(s) 148
BsiSI CCGG 2 cut(s) 414, 570
BslFI GGGAC 2 cut(s) 288, 911
BslI CCNNNNNNNGG 2 cut(s) 160, 161
BsmAI GTCTC 3 cut(s) 795, 821, 1003
BsmBI CGTCTC 1 cut(s) 821
BsmFI GGGAC 2 cut(s) 288, 911
BsnI GGCC 1 cut(s) 756
Bsp119I TTCGAA 2 cut(s) 143, 890
Bsp1286I GDGCHC 1 cut(s) 358
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 5 cut(s) 128, 418, 478, 678, 1084
BspACI CCGC 3 cut(s) 751, 753, 980
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 4 cut(s) 817, 874, 910, 1090
BspFNI CGCG 1 cut(s) 753
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 2 cut(s) 150, 714
BspMI ACCTGC 2 cut(s) 777, 1102
BspT104I TTCGAA 2 cut(s) 143, 890
BspT107I GGYRCC 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 569
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 1 cut(s) 238
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 4 cut(s) 708, 751, 873, 1151
BssMI GATC 5 cut(s) 128, 418, 478, 678, 1084
BssNI GRCGYC 2 cut(s) 106, 149
BssT1I CCWWGG 1 cut(s) 1151
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 1 cut(s) 832
BstACI GRCGYC 2 cut(s) 106, 149
BstAPI GCANNNNNTGC 1 cut(s) 977
BstAUI TGTACA 1 cut(s) 498
BstBI TTCGAA 2 cut(s) 143, 890
BstC8I GCNNGC 6 cut(s) 803, 912, 961, 965, 969, 973
BstDEI CTNAG 5 cut(s) 278, 825, 882, 918, 1098
BstDSI CCRYGG 1 cut(s) 751
BstF5I GGATG 2 cut(s) 293, 443
BstFNI CGCG 1 cut(s) 753
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 2 cut(s) 151, 951
BstKTI GATC 5 cut(s) 131, 421, 481, 681, 1087
BstMAI GTCTC 3 cut(s) 795, 821, 1003
BstMBI GATC 5 cut(s) 128, 418, 478, 678, 1084
BstMWI GCNNNNNNNGC 4 cut(s) 948, 960, 977, 1013
BstNI CCWGG 1 cut(s) 710
BstNSI RCATGY 3 cut(s) 459, 477, 663
BstSCI CCNGG 2 cut(s) 412, 708
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 2 cut(s) 963, 1105
BstV2I GAAGAC 3 cut(s) 158, 331, 624
BsuRI GGCC 1 cut(s) 756
BtgI CCRYGG 1 cut(s) 751
BtsCI GGATG 2 cut(s) 293, 443
BtsIMutI CAGTG 1 cut(s) 1113
BveI ACCTGC 2 cut(s) 777, 1102
Cac8I GCNNGC 6 cut(s) 803, 912, 961, 965, 969, 973
CciI TCATGA 1 cut(s) 358
CfoI GCGC 2 cut(s) 151, 951
Cfr10I RCCGGY 1 cut(s) 569
Cfr42I CCGCGG 1 cut(s) 754
CseI GACGC 1 cut(s) 95
Csp6I GTAC 6 cut(s) 113, 499, 522, 997, 1136, 1148
CviAII CATG 7 cut(s) 359, 456, 474, 660, 773, 931, 946
CviQI GTAC 6 cut(s) 113, 499, 522, 997, 1136, 1148
DdeI CTNAG 5 cut(s) 278, 825, 882, 918, 1098
DinI GGCGCC 1 cut(s) 150
DpnI GATC 5 cut(s) 130, 420, 480, 680, 1086
DpnII GATC 5 cut(s) 128, 418, 478, 678, 1084
DraI TTTAAA 1 cut(s) 649
DrdI GACNNNNNNGTC 1 cut(s) 512
DseDI GACNNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 754
Eco130I CCWWGG 1 cut(s) 1151
Eco24I GRGCYC 1 cut(s) 358
Eco57I CTGAAG 3 cut(s) 425, 507, 749
EcoRII CCWGG 1 cut(s) 708
EcoT14I CCWWGG 1 cut(s) 1151
EcoT22I ATGCAT 1 cut(s) 665
EcoT38I GRGCYC 1 cut(s) 358
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 1151
Esp3I CGTCTC 1 cut(s) 821
FaeI CATG 7 cut(s) 362, 459, 477, 663, 776, 934, 949
FaqI GGGAC 2 cut(s) 288, 911
FatI CATG 7 cut(s) 358, 455, 473, 659, 772, 930, 945
Fnu4HI GCNGC 5 cut(s) 751, 754, 952, 981, 1119
FokI GGATG 2 cut(s) 300, 430
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 5 cut(s) 751, 754, 952, 981, 1119
FspI TGCGCA 1 cut(s) 950
GlaI GCGC 2 cut(s) 150, 950
GluI GCNGC 5 cut(s) 751, 754, 952, 981, 1119
GsaI CCCAGC 1 cut(s) 621
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 1 cut(s) 756
HapII CCGG 2 cut(s) 414, 570
HgaI GACGC 1 cut(s) 95
HhaI GCGC 2 cut(s) 151, 951
Hin1I GRCGYC 2 cut(s) 106, 149
Hin1II CATG 7 cut(s) 362, 459, 477, 663, 776, 934, 949
Hin6I GCGC 2 cut(s) 149, 949
HinP1I GCGC 2 cut(s) 149, 949
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 7 cut(s) 14, 60, 431, 629, 788, 878, 986
HpaII CCGG 2 cut(s) 414, 570
HphI GGTGA 1 cut(s) 302
Hpy188I TCNGA 7 cut(s) 487, 518, 628, 826, 883, 919, 1099
Hpy188III TCNNGA 5 cut(s) 359, 667, 676, 727, 1088
Hpy99I CGWCG 1 cut(s) 129
HpyAV CCTTC 4 cut(s) 34, 139, 150, 180
HpyCH4III ACNGT 1 cut(s) 832
HpyCH4IV ACGT 3 cut(s) 85, 115, 995
HpyF10VI GCNNNNNNNGC 4 cut(s) 948, 960, 977, 1013
HpyF3I CTNAG 5 cut(s) 278, 825, 882, 918, 1098
HpySE526I ACGT 3 cut(s) 85, 115, 995
Hsp92I GRCGYC 2 cut(s) 106, 149
Hsp92II CATG 7 cut(s) 362, 459, 477, 663, 776, 934, 949
HspAI GCGC 2 cut(s) 149, 949
KasI GGCGCC 1 cut(s) 148
KspI CCGCGG 1 cut(s) 754
Kzo9I GATC 5 cut(s) 128, 418, 478, 678, 1084
LmnI GCTCC 1 cut(s) 614
Lsp1109I GCAGC 2 cut(s) 963, 1105
LweI GCATC 4 cut(s) 452, 672, 835, 994
MaeII ACGT 3 cut(s) 85, 115, 995
MaeIII GTNAC 5 cut(s) 86, 448, 637, 655, 695
MalI GATC 5 cut(s) 130, 420, 480, 680, 1086
MboI GATC 5 cut(s) 128, 418, 478, 678, 1084
MfeI CAATTG 2 cut(s) 6, 156
MhlI GDGCHC 1 cut(s) 358
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 5 cut(s) 54, 425, 638, 797, 887
MmeI TCCRAC 2 cut(s) 491, 496
MnlI CCTC 8 cut(s) 154, 287, 316, 421, 512, 868, 925, 1084
Mph1103I ATGCAT 1 cut(s) 665
MseI TTAA 7 cut(s) 45, 78, 366, 381, 648, 795, 852
MslI CAYNNNNRTG 1 cut(s) 935
MspA1I CMGCKG 2 cut(s) 753, 805
MspI CCGG 2 cut(s) 414, 570
MspR9I CCNGG 2 cut(s) 414, 710
MunI CAATTG 2 cut(s) 6, 156
MvaI CCWGG 1 cut(s) 710
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 4 cut(s) 948, 960, 977, 1013
NarI GGCGCC 1 cut(s) 149
NciI CCSGG 1 cut(s) 414
NdeII GATC 5 cut(s) 128, 418, 478, 678, 1084
NlaIII CATG 7 cut(s) 362, 459, 477, 663, 776, 934, 949
NlaIV GGNNCC 2 cut(s) 150, 714
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 3 cut(s) 86, 637, 655
NsbI TGCGCA 1 cut(s) 950
NsiI ATGCAT 1 cut(s) 665
NspI RCATGY 3 cut(s) 459, 477, 663
NspV TTCGAA 2 cut(s) 143, 890
PagI TCATGA 1 cut(s) 358
PciI ACATGT 2 cut(s) 455, 473
PcsI WCGNNNNNNNCGW 1 cut(s) 121
PfeI GAWTC 2 cut(s) 14, 986
PkrI GCNGC 5 cut(s) 752, 755, 953, 982, 1120
PleI GAGTC 5 cut(s) 54, 425, 637, 796, 886
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 5 cut(s) 54, 425, 637, 796, 886
PscI ACATGT 2 cut(s) 455, 473
Psp6I CCWGG 1 cut(s) 708
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 2 cut(s) 150, 714
PvuII CAGCTG 1 cut(s) 805
RsaI GTAC 6 cut(s) 114, 500, 523, 998, 1137, 1149
RsaNI GTAC 6 cut(s) 113, 499, 522, 997, 1136, 1148
RseI CAYNNNNRTG 1 cut(s) 935
SacII CCGCGG 1 cut(s) 754
SaqAI TTAA 7 cut(s) 45, 78, 366, 381, 648, 795, 852
SatI GCNGC 5 cut(s) 751, 754, 952, 981, 1119
Sau3AI GATC 5 cut(s) 128, 418, 478, 678, 1084
ScaI AGTACT 1 cut(s) 1137
SchI GAGTC 5 cut(s) 54, 425, 638, 797, 887
ScrFI CCNGG 2 cut(s) 414, 710
SduI GDGCHC 1 cut(s) 358
SfaNI GCATC 4 cut(s) 452, 672, 835, 994
SfoI GGCGCC 1 cut(s) 150
Sfr303I CCGCGG 1 cut(s) 754
SfuI TTCGAA 2 cut(s) 143, 890
SgrBI CCGCGG 1 cut(s) 754
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SsiI CCGC 3 cut(s) 751, 753, 980
SspDI GGCGCC 1 cut(s) 148
SspI AATATT 2 cut(s) 737, 838
StyD4I CCNGG 2 cut(s) 412, 708
StyI CCWWGG 1 cut(s) 1151
TaaI ACNGT 1 cut(s) 832
TaiI ACGT 3 cut(s) 88, 118, 998
TaqI TCGA 4 cut(s) 143, 173, 666, 890
TaqII GACCGA 1 cut(s) 251
TatI WGTACW 2 cut(s) 498, 1135
TauI GCSGC 3 cut(s) 753, 756, 983
TfiI GAWTC 2 cut(s) 14, 986
Tru1I TTAA 7 cut(s) 45, 78, 366, 381, 648, 795, 852
Tru9I TTAA 7 cut(s) 45, 78, 366, 381, 648, 795, 852
TscAI CASTG 1 cut(s) 1113
TseFI GTSAC 3 cut(s) 86, 637, 655
TseI GCWGC 2 cut(s) 951, 1118
Tsp45I GTSAC 3 cut(s) 86, 637, 655
TspDTI ATGAA 8 cut(s) 69, 243, 296, 375, 579, 789, 934, 947
TspGWI ACGGA 1 cut(s) 436
TspRI CASTG 1 cut(s) 1113
XapI RAATTY 4 cut(s) 20, 362, 603, 854
XceI RCATGY 3 cut(s) 459, 477, 663
XcmI CCANNNNNNNNNTGG 1 cut(s) 809
ZrmI AGTACT 1 cut(s) 1137
Zsp2I ATGCAT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.