Rw6G035090

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017257
Physical Location & Seq
Forward (+)
59655498 .. 59661829
6332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035090.1

Sequence Viewer

Length: 1224 bp
ATGGGCCGGCACAAGCCCGACACGATAATAAATGGGTCGGCCAAAGCACGGCACGAAGCACGACTAGGCCCGCTTAAAATGGGTAGGGCCGGGTCGGGCAGGCCCGTTTGCCACCTCTACCCACCACAAGCAGTTGCTACATGCGTGAGCTTTAATCAACCCTTGCCTTGTTTGCCGAAACGATTAAAGAGAGCCATGTCTGAGAAATCATCACTTCTGGAAAGAGCTGAGGAACTTGCAGATCAAATGCCAGTAAAATGTCCTCATTTTGTCAAAGTGATGCTTCCATCACATGTTGCTGGAGGCTTCTGGCTGGGTCTTCCTTCGACATTCTGCCACAAACATATAGGAACAAGCGACCAGACGATTATATTGGAAGATGAAACTGGCGATAAGTTCAAAACAACATATCTCGGTCATAAGATGGGACTGAGTGCTGGATGGAGAGGGTTTTCCATTCATCACCATTTGCTTGAGGGAGATGTTTTGGTCTTCCAAAAAGTCACGGCCAATGAATTTAAGGTTTATATAGTTAAACCAAATGGTTCAAAAAAACTGAAGTTTCCCGGTAAGTTGTCTAAAAATTCAACAGAGAGTGAAGAAGAAGATATGTTGAATTATATTGACAATTATGAAGCCGGTGCAGAGAGCGAAGATGATATTGACCCTGAATTTTTCAAAGGAGCTGGGCTTTCGGAGTCTTCTGTCACTTGTTTTGAAGAAGTGACATGCATCGAGAAGTTCAAGATCATATTCAATGGCTGTAACATCAATTCCCAGGTTCCTAAACTCGTCTTGAAGAAATATTATGAGCTTTGCCGCAGCCAAAACTCATACCTGCATGAAAATCTTGTGGAGTCTCTTAATAGCCAACTGGTTGCTGGACTAATCTCTGAGACGGTCAATATTGCAGATGCAATTAAATTCTGCGAGATTACCACCGAGGAGTCTGAGTTTTCGACGTGGGACAAGACCTTGCAAGCCTCTGAGATGTTAGGCATGAATGTGGGCTTCATGCGCAGCCGAGCGAGCAAGCTTGCAAGCCTTGCGGCAGAATCAAAGACGTACAAAGACGCGAGACTTGCAAGAGAGCAAGAAGAAGCAGAGTTTCGGGTCAGGTTGGCTGACTTTTTGAAAGAAAAAGATAAAGCAGACCGGGAGGGGTCTGAGAAATCAGTGCAGGTTGCTACAAGTTTCAAACAAGTGACAAATGTACCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

407

Amino Acids

45.51

Weight (kDa)

6.65

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 90 - 178 1.2e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017257)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41030 FvH4_2g41030
rosa_chinensis RchiOBHm_Chr6g0299531
rosa_laevigata RLG00000011418
rosa_multiflora Rmu_sc0004064.1_g000007
rosa_roxburghii Rroxscaffold_7G00168580
rosa_samantha Rh6AG400900 Rh6AG401000 Rh6BG409500 Rh6CG414900 Rh6DG402000 Rh6DG402100
rosa_wichuraiana Rw6G035090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1019
Acc36I ACCTGC 2 cut(s) 846, 1171
AccII CGCG 1 cut(s) 1076
AciI CCGC 3 cut(s) 71, 820, 1049
AcoI YGGCCR 2 cut(s) 39, 507
AcsI RAATTY 4 cut(s) 515, 583, 671, 923
AcuI CTGAAG 1 cut(s) 578
AfaI GTAC 2 cut(s) 1067, 1215
AfiI CCNNNNNNNGG 1 cut(s) 48
AflIII ACRYGT 1 cut(s) 292
AjiI CACGTC 1 cut(s) 963
AjnI CCWGG 1 cut(s) 777
AluBI AGCT 5 cut(s) 150, 227, 686, 814, 1036
AluI AGCT 5 cut(s) 150, 227, 686, 814, 1036
Alw26I GTCTC 3 cut(s) 864, 890, 1072
AoxI GGCC 6 cut(s) 4, 39, 67, 87, 101, 507
ApeKI GCWGC 2 cut(s) 822, 1020
ApoI RAATTY 4 cut(s) 515, 583, 671, 923
AspLEI GCGC 1 cut(s) 1020
AspS9I GGNCC 4 cut(s) 4, 68, 87, 102
AsuC2I CCSGG 3 cut(s) 91, 567, 1157
AsuHPI GGTGA 1 cut(s) 455
BaeI ACNNNNGTAYC 1 cut(s) 1197
BbsI GAAGAC 3 cut(s) 311, 484, 693
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 834, 1032
BccI CCATC 3 cut(s) 295, 418, 435
BceAI ACGGC 2 cut(s) 65, 522
BciT130I CCWGG 1 cut(s) 779
BcnI CCSGG 3 cut(s) 91, 567, 1157
BcoDI GTCTC 3 cut(s) 864, 890, 1072
BfaI CTAG 1 cut(s) 65
BfuAI ACCTGC 2 cut(s) 846, 1171
BisI GCNGC 4 cut(s) 820, 823, 1021, 1050
BlsI GCNGC 4 cut(s) 821, 824, 1022, 1051
Bme1390I CCNGG 4 cut(s) 91, 567, 779, 1157
BmgBI CACGTC 1 cut(s) 963
BmgT120I GGNCC 4 cut(s) 4, 68, 87, 102
BmiI GGNNCC 1 cut(s) 783
BmrFI CCNGG 4 cut(s) 91, 567, 779, 1157
BmsI GCATC 3 cut(s) 270, 741, 904
BpiI GAAGAC 3 cut(s) 311, 484, 693
BpmI CTGGAG 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 3 cut(s) 91, 567, 1157
BsaJI CCNNGG 3 cut(s) 777, 942, 1217
BsaXI ACNNNNNCTCC 2 cut(s) 938, 968
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse118I RCCGGY 2 cut(s) 6, 638
Bse1I ACTGG 3 cut(s) 251, 391, 879
BseBI CCWGG 1 cut(s) 779
BseDI CCNNGG 3 cut(s) 777, 942, 1217
BseGI GGATG 1 cut(s) 446
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMII CTCAG 7 cut(s) 192, 219, 422, 885, 942, 978, 1158
BseNI ACTGG 3 cut(s) 251, 391, 879
BseRI GAGGAG 1 cut(s) 959
BseXI GCAGC 2 cut(s) 834, 1032
BseYI CCCAGC 2 cut(s) 313, 686
BsgI GTGCAG 2 cut(s) 663, 1199
Bsh1236I CGCG 1 cut(s) 1076
BshFI GGCC 6 cut(s) 6, 41, 69, 89, 103, 509
BsiSI CCGG 5 cut(s) 7, 90, 567, 639, 1156
BslFI GGGAC 2 cut(s) 441, 980
BslI CCNNNNNNNGG 1 cut(s) 48
BsmAI GTCTC 3 cut(s) 864, 890, 1072
BsmBI CGTCTC 1 cut(s) 890
BsmFI GGGAC 2 cut(s) 441, 980
BsnI GGCC 6 cut(s) 6, 41, 69, 89, 103, 509
Bsp143I GATC 2 cut(s) 241, 747
BspACI CCGC 3 cut(s) 71, 820, 1049
BspANI GGCC 6 cut(s) 6, 41, 69, 89, 103, 509
BspCNI CTCAG 7 cut(s) 193, 220, 423, 886, 943, 979, 1159
BspFNI CGCG 1 cut(s) 1076
BspLI GGNNCC 1 cut(s) 783
BspMI ACCTGC 2 cut(s) 846, 1171
BsrFI RCCGGY 2 cut(s) 6, 638
BsrI ACTGG 3 cut(s) 251, 391, 879
BssAI RCCGGY 2 cut(s) 6, 638
BssECI CCNNGG 3 cut(s) 777, 942, 1217
BssMI GATC 2 cut(s) 241, 747
BssT1I CCWWGG 1 cut(s) 1217
Bst2UI CCWGG 1 cut(s) 779
Bst4CI ACNGT 1 cut(s) 901
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstC8I GCNNGC 8 cut(s) 8, 71, 101, 981, 1030, 1034, 1038, 1042
BstDEI CTNAG 7 cut(s) 201, 228, 431, 894, 951, 987, 1167
BstF5I GGATG 1 cut(s) 446
BstFNI CGCG 1 cut(s) 1076
BstHHI GCGC 1 cut(s) 1020
BstKTI GATC 2 cut(s) 244, 750
BstMAI GTCTC 3 cut(s) 864, 890, 1072
BstMBI GATC 2 cut(s) 241, 747
BstMWI GCNNNNNNNGC 5 cut(s) 172, 1017, 1029, 1046, 1082
BstNI CCWGG 1 cut(s) 779
BstNSI RCATGY 3 cut(s) 144, 296, 732
BstSCI CCNGG 4 cut(s) 89, 565, 777, 1155
BstUI CGCG 1 cut(s) 1076
BstV1I GCAGC 2 cut(s) 834, 1032
BstV2I GAAGAC 3 cut(s) 311, 484, 693
BsuRI GGCC 6 cut(s) 6, 41, 69, 89, 103, 509
BtrI CACGTC 1 cut(s) 963
BtsCI GGATG 1 cut(s) 446
BtsIMutI CAGTG 1 cut(s) 1182
BveI ACCTGC 2 cut(s) 846, 1171
Cac8I GCNNGC 8 cut(s) 8, 71, 101, 981, 1030, 1034, 1038, 1042
CfoI GCGC 1 cut(s) 1020
Cfr10I RCCGGY 2 cut(s) 6, 638
Cfr13I GGNCC 4 cut(s) 4, 68, 87, 102
CseI GACGC 1 cut(s) 1082
Csp6I GTAC 2 cut(s) 1066, 1214
CviAII CATG 7 cut(s) 141, 196, 293, 729, 842, 1000, 1015
CviQI GTAC 2 cut(s) 1066, 1214
DdeI CTNAG 7 cut(s) 201, 228, 431, 894, 951, 987, 1167
DpnI GATC 2 cut(s) 243, 749
DpnII GATC 2 cut(s) 241, 747
EaeI YGGCCR 2 cut(s) 39, 507
Eco130I CCWWGG 1 cut(s) 1217
Eco57I CTGAAG 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 777
EcoT14I CCWWGG 1 cut(s) 1217
EcoT22I ATGCAT 1 cut(s) 734
ErhI CCWWGG 1 cut(s) 1217
Esp3I CGTCTC 1 cut(s) 890
FaeI CATG 7 cut(s) 144, 199, 296, 732, 845, 1003, 1018
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 2 cut(s) 441, 980
FatI CATG 7 cut(s) 140, 195, 292, 728, 841, 999, 1014
FauI CCCGC 1 cut(s) 78
Fnu4HI GCNGC 4 cut(s) 820, 823, 1021, 1050
FokI GGATG 1 cut(s) 453
Fsp4HI GCNGC 4 cut(s) 820, 823, 1021, 1050
FspBI CTAG 1 cut(s) 65
FspI TGCGCA 1 cut(s) 1019
GlaI GCGC 1 cut(s) 1019
GluI GCNGC 4 cut(s) 820, 823, 1021, 1050
GsaI CCCAGC 2 cut(s) 317, 690
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 6 cut(s) 6, 41, 69, 89, 103, 509
HapII CCGG 5 cut(s) 7, 90, 567, 639, 1156
HgaI GACGC 1 cut(s) 1082
HhaI GCGC 1 cut(s) 1020
Hin1II CATG 7 cut(s) 144, 199, 296, 732, 845, 1003, 1018
Hin6I GCGC 1 cut(s) 1018
HinP1I GCGC 1 cut(s) 1018
HindIII AAGCTT 1 cut(s) 1034
HinfI GANTC 4 cut(s) 698, 857, 947, 1055
HpaII CCGG 5 cut(s) 7, 90, 567, 639, 1156
HphI GGTGA 1 cut(s) 455
Hpy188I TCNGA 6 cut(s) 202, 697, 895, 952, 988, 1168
Hpy188III TCNNGA 4 cut(s) 218, 736, 745, 796
Hpy99I CGWCG 1 cut(s) 964
HpyAV CCTTC 1 cut(s) 333
HpyCH4III ACNGT 1 cut(s) 901
HpyCH4IV ACGT 2 cut(s) 962, 1064
HpyF10VI GCNNNNNNNGC 5 cut(s) 172, 1017, 1029, 1046, 1082
HpyF3I CTNAG 7 cut(s) 201, 228, 431, 894, 951, 987, 1167
HpySE526I ACGT 2 cut(s) 962, 1064
Hsp92II CATG 7 cut(s) 144, 199, 296, 732, 845, 1003, 1018
HspAI GCGC 1 cut(s) 1018
KroI GCCGGC 1 cut(s) 6
KroNI GCCGGC 1 cut(s) 8
Kzo9I GATC 2 cut(s) 241, 747
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 2 cut(s) 834, 1032
LweI GCATC 3 cut(s) 270, 741, 904
MaeI CTAG 1 cut(s) 65
MaeII ACGT 2 cut(s) 962, 1064
MaeIII GTNAC 5 cut(s) 502, 706, 724, 764, 1204
MalI GATC 2 cut(s) 243, 749
MboI GATC 2 cut(s) 241, 747
MluCI AATT 8 cut(s) 515, 583, 616, 628, 671, 772, 918, 923
MlyI GAGTC 3 cut(s) 707, 866, 956
MnlI CCTC 9 cut(s) 125, 223, 273, 296, 440, 469, 937, 994, 1153
Mph1103I ATGCAT 1 cut(s) 734
MroNI GCCGGC 1 cut(s) 6
MseI TTAA 7 cut(s) 75, 153, 185, 519, 534, 864, 921
MslI CAYNNNNRTG 1 cut(s) 1004
MspI CCGG 5 cut(s) 7, 90, 567, 639, 1156
MspR9I CCNGG 4 cut(s) 91, 567, 779, 1157
MvaI CCWGG 1 cut(s) 779
MvnI CGCG 1 cut(s) 1076
MwoI GCNNNNNNNGC 5 cut(s) 172, 1017, 1029, 1046, 1082
NaeI GCCGGC 1 cut(s) 8
NciI CCSGG 3 cut(s) 91, 567, 1157
NdeII GATC 2 cut(s) 241, 747
NgoMIV GCCGGC 1 cut(s) 6
NlaIII CATG 7 cut(s) 144, 199, 296, 732, 845, 1003, 1018
NlaIV GGNNCC 1 cut(s) 783
NmeAIII GCCGAG 1 cut(s) 1049
NmuCI GTSAC 4 cut(s) 502, 706, 724, 1204
NsbI TGCGCA 1 cut(s) 1019
NsiI ATGCAT 1 cut(s) 734
NspI RCATGY 3 cut(s) 144, 296, 732
PciI ACATGT 1 cut(s) 292
PdiI GCCGGC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 1055
PkrI GCNGC 4 cut(s) 821, 824, 1022, 1051
PleI GAGTC 3 cut(s) 706, 865, 955
PpsI GAGTC 3 cut(s) 706, 865, 955
PscI ACATGT 1 cut(s) 292
Psp6I CCWGG 1 cut(s) 777
PspFI CCCAGC 2 cut(s) 313, 686
PspGI CCWGG 1 cut(s) 777
PspN4I GGNNCC 1 cut(s) 783
PspPI GGNCC 4 cut(s) 4, 68, 87, 102
RsaI GTAC 2 cut(s) 1067, 1215
RsaNI GTAC 2 cut(s) 1066, 1214
RseI CAYNNNNRTG 1 cut(s) 1004
SaqAI TTAA 7 cut(s) 75, 153, 185, 519, 534, 864, 921
SatI GCNGC 4 cut(s) 820, 823, 1021, 1050
Sau3AI GATC 2 cut(s) 241, 747
Sau96I GGNCC 4 cut(s) 4, 68, 87, 102
SchI GAGTC 3 cut(s) 707, 866, 956
ScrFI CCNGG 4 cut(s) 91, 567, 779, 1157
SfaNI GCATC 3 cut(s) 270, 741, 904
SmiMI CAYNNNNRTG 1 cut(s) 1004
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 8 cut(s) 515, 583, 616, 628, 671, 772, 918, 923
SsiI CCGC 3 cut(s) 71, 820, 1049
SspI AATATT 2 cut(s) 806, 907
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 4 cut(s) 89, 565, 777, 1155
StyI CCWWGG 1 cut(s) 1217
TaaI ACNGT 1 cut(s) 901
TaiI ACGT 2 cut(s) 965, 1067
TaqI TCGA 3 cut(s) 326, 735, 959
TaqII GACCGA 1 cut(s) 404
TasI AATT 8 cut(s) 515, 583, 616, 628, 671, 772, 918, 923
TauI GCSGC 2 cut(s) 822, 1052
TfiI GAWTC 1 cut(s) 1055
Tru1I TTAA 7 cut(s) 75, 153, 185, 519, 534, 864, 921
Tru9I TTAA 7 cut(s) 75, 153, 185, 519, 534, 864, 921
TscAI CASTG 1 cut(s) 1182
TseFI GTSAC 4 cut(s) 502, 706, 724, 1204
TseI GCWGC 2 cut(s) 822, 1020
Tsp45I GTSAC 4 cut(s) 502, 706, 724, 1204
TspDTI ATGAA 7 cut(s) 396, 449, 528, 648, 858, 1003, 1016
TspRI CASTG 1 cut(s) 1182
XapI RAATTY 4 cut(s) 515, 583, 671, 923
XceI RCATGY 3 cut(s) 144, 296, 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 878
XspI CTAG 1 cut(s) 65
Zsp2I ATGCAT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.