Rh6DG402100

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0017257
Physical Location & Seq
Forward (+)
59078246 .. 59078962
717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG402100.1

Sequence Viewer

Length: 717 bp
ATGGCATCCGTTACTACATGTAGTATTCCAACAACATGTGATCTTTCTGAAGATATATTGTACATTGACAATTATGTCGGAGGTACAGAGAGTGAAGAAGATATGTTCAATTATATTGACAATTATGAAGCCGGTGCAGAGAGTGAAGATGATATTGTCCCTGAAATTTTCAAAGGAGCTGGGCTTTCGGAGTCTTCTGTCACTTGTTTTAAAGAAGTGACATGCATCGAGAAGTTCAAGATCATATTCAATGGCTGTAACATCAATTCCCAGGTTCCTAAACTCGTCCTGAAGAAATATTATGAGCTTTGCCGCGGCCAAAACTCATACCTGCATGAAAATCTTGTGGAGTCTCTTAACAGCCAGCTGGTTGCTGGACTAATCTCTGAGACGGTCAATATTGCAGATGCAATTAAATTCTGCAAGATTACCACCGAGGAGTCTGAGTTTTCGAAGTGGGACAAGACCTTGCAAGCCTCTGAGATGTTAGGCATGAATGTGGGCTTCATGCGCAGCCGAGCGAGCAAGCTTGCAAGCCTTGCGGCAGAATCAAAGACGTACAAAGACGCGAGACTTGCAAGAGAGCAAGAAGAAGCAGAGTTTCGGGTCAGGTTGGCTGACTTTTTGAAAGAAAAAGATAAAGCAGATCGGGAGGGGTCTGAGAAATCAGTGCAGGTTGCTGCAAGTTTCAAACAAGTGACAAATGTACCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

238

Amino Acids

26.65

Weight (kDa)

4.71

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017257)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41030 FvH4_2g41030
rosa_chinensis RchiOBHm_Chr6g0299531
rosa_laevigata RLG00000011418
rosa_multiflora Rmu_sc0004064.1_g000007
rosa_roxburghii Rroxscaffold_7G00168580
rosa_samantha Rh6AG400900 Rh6AG401000 Rh6BG409500 Rh6CG414900 Rh6DG402000 Rh6DG402100
rosa_wichuraiana Rw6G035090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
Acc16I TGCGCA 1 cut(s) 512
Acc36I ACCTGC 2 cut(s) 339, 664
AccII CGCG 2 cut(s) 315, 569
AciI CCGC 3 cut(s) 313, 315, 542
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 2 cut(s) 165, 416
AcuI CTGAAG 2 cut(s) 69, 311
AfaI GTAC 4 cut(s) 62, 85, 560, 708
AflIII ACRYGT 2 cut(s) 17, 35
AgsI TTSAA 6 cut(s) 109, 172, 238, 250, 628, 691
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 4 cut(s) 179, 307, 367, 529
AluI AGCT 4 cut(s) 179, 307, 367, 529
Alw26I GTCTC 3 cut(s) 357, 383, 565
AoxI GGCC 1 cut(s) 316
ApeKI GCWGC 2 cut(s) 513, 680
ApoI RAATTY 2 cut(s) 165, 416
AspLEI GCGC 1 cut(s) 513
AsuII TTCGAA 1 cut(s) 452
BaeI ACNNNNGTAYC 1 cut(s) 690
BbsI GAAGAC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 525, 667
BciT130I CCWGG 1 cut(s) 272
BcoDI GTCTC 3 cut(s) 357, 383, 565
BfuAI ACCTGC 2 cut(s) 339, 664
BisI GCNGC 5 cut(s) 313, 316, 514, 543, 681
BlsI GCNGC 5 cut(s) 314, 317, 515, 544, 682
Bme1390I CCNGG 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 3 cut(s) 14, 234, 397
BpiI GAAGAC 1 cut(s) 186
Bpu14I TTCGAA 1 cut(s) 452
BsaJI CCNNGG 4 cut(s) 270, 313, 435, 710
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bse118I RCCGGY 1 cut(s) 131
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 4 cut(s) 270, 313, 435, 710
BseGI GGATG 1 cut(s) 5
BseMII CTCAG 4 cut(s) 378, 435, 471, 651
BseRI GAGGAG 1 cut(s) 452
BseXI GCAGC 2 cut(s) 525, 667
BseYI CCCAGC 1 cut(s) 179
BsgI GTGCAG 2 cut(s) 156, 692
Bsh1236I CGCG 2 cut(s) 315, 569
BshFI GGCC 1 cut(s) 318
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 2 cut(s) 143, 473
BsmAI GTCTC 3 cut(s) 357, 383, 565
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 143, 473
BsnI GGCC 1 cut(s) 318
Bsp119I TTCGAA 1 cut(s) 452
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 3 cut(s) 40, 240, 646
BspACI CCGC 3 cut(s) 313, 315, 542
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 4 cut(s) 379, 436, 472, 652
BspFNI CGCG 2 cut(s) 315, 569
BspLI GGNNCC 1 cut(s) 276
BspMI ACCTGC 2 cut(s) 339, 664
BspT104I TTCGAA 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 60
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 4 cut(s) 270, 313, 435, 710
BssMI GATC 3 cut(s) 40, 240, 646
BssT1I CCWWGG 1 cut(s) 710
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 394
BstAPI GCANNNNNTGC 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 6 cut(s) 365, 474, 523, 527, 531, 535
BstDEI CTNAG 4 cut(s) 387, 444, 480, 660
BstDSI CCRYGG 1 cut(s) 313
BstF5I GGATG 1 cut(s) 5
BstFNI CGCG 2 cut(s) 315, 569
BstHHI GCGC 1 cut(s) 513
BstKTI GATC 3 cut(s) 43, 243, 649
BstMAI GTCTC 3 cut(s) 357, 383, 565
BstMBI GATC 3 cut(s) 40, 240, 646
BstMWI GCNNNNNNNGC 4 cut(s) 510, 522, 539, 575
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 3 cut(s) 21, 39, 225
BstSCI CCNGG 1 cut(s) 270
BstUI CGCG 2 cut(s) 315, 569
BstV1I GCAGC 2 cut(s) 525, 667
BstV2I GAAGAC 1 cut(s) 186
BsuRI GGCC 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 313
BtsCI GGATG 1 cut(s) 5
BtsIMutI CAGTG 1 cut(s) 675
BveI ACCTGC 2 cut(s) 339, 664
Cac8I GCNNGC 6 cut(s) 365, 474, 523, 527, 531, 535
CfoI GCGC 1 cut(s) 513
Cfr10I RCCGGY 1 cut(s) 131
Cfr42I CCGCGG 1 cut(s) 316
CseI GACGC 1 cut(s) 575
Csp6I GTAC 4 cut(s) 61, 84, 559, 707
CviAII CATG 6 cut(s) 18, 36, 222, 335, 493, 508
CviQI GTAC 4 cut(s) 61, 84, 559, 707
DdeI CTNAG 4 cut(s) 387, 444, 480, 660
DpnI GATC 3 cut(s) 42, 242, 648
DpnII GATC 3 cut(s) 40, 240, 646
DraI TTTAAA 1 cut(s) 211
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 710
Eco57I CTGAAG 2 cut(s) 69, 311
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 710
EcoT22I ATGCAT 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 710
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 6 cut(s) 21, 39, 225, 338, 496, 511
FaqI GGGAC 2 cut(s) 143, 473
FatI CATG 6 cut(s) 17, 35, 221, 334, 492, 507
Fnu4HI GCNGC 5 cut(s) 313, 316, 514, 543, 681
Fsp4HI GCNGC 5 cut(s) 313, 316, 514, 543, 681
FspI TGCGCA 1 cut(s) 512
GlaI GCGC 1 cut(s) 512
GluI GCNGC 5 cut(s) 313, 316, 514, 543, 681
GsaI CCCAGC 1 cut(s) 183
HaeIII GGCC 1 cut(s) 318
HapII CCGG 1 cut(s) 132
HgaI GACGC 1 cut(s) 575
HhaI GCGC 1 cut(s) 513
Hin1II CATG 6 cut(s) 21, 39, 225, 338, 496, 511
Hin6I GCGC 1 cut(s) 511
HinP1I GCGC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 4 cut(s) 191, 350, 440, 548
HpaII CCGG 1 cut(s) 132
Hpy188I TCNGA 7 cut(s) 49, 80, 190, 388, 445, 481, 661
Hpy188III TCNNGA 4 cut(s) 229, 238, 289, 650
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 557
HpyF10VI GCNNNNNNNGC 4 cut(s) 510, 522, 539, 575
HpyF3I CTNAG 4 cut(s) 387, 444, 480, 660
HpySE526I ACGT 1 cut(s) 557
Hsp92II CATG 6 cut(s) 21, 39, 225, 338, 496, 511
HspAI GCGC 1 cut(s) 511
KspI CCGCGG 1 cut(s) 316
Kzo9I GATC 3 cut(s) 40, 240, 646
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 2 cut(s) 525, 667
LweI GCATC 3 cut(s) 14, 234, 397
MaeII ACGT 1 cut(s) 557
MaeIII GTNAC 5 cut(s) 10, 199, 217, 257, 697
MalI GATC 3 cut(s) 42, 242, 648
MboI GATC 3 cut(s) 40, 240, 646
MboII GAAGA 7 cut(s) 62, 107, 110, 158, 186, 304, 602
MluCI AATT 7 cut(s) 70, 109, 121, 165, 265, 411, 416
MlyI GAGTC 3 cut(s) 200, 359, 449
MmeI TCCRAC 2 cut(s) 53, 58
MnlI CCTC 4 cut(s) 74, 430, 487, 646
Mph1103I ATGCAT 1 cut(s) 227
MseI TTAA 3 cut(s) 210, 357, 414
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 2 cut(s) 315, 367
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 1 cut(s) 272
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 2 cut(s) 315, 569
MwoI GCNNNNNNNGC 4 cut(s) 510, 522, 539, 575
NdeII GATC 3 cut(s) 40, 240, 646
NlaIII CATG 6 cut(s) 21, 39, 225, 338, 496, 511
NlaIV GGNNCC 1 cut(s) 276
NmeAIII GCCGAG 1 cut(s) 542
NmuCI GTSAC 3 cut(s) 199, 217, 697
NsbI TGCGCA 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 227
NspI RCATGY 3 cut(s) 21, 39, 225
NspV TTCGAA 1 cut(s) 452
PciI ACATGT 2 cut(s) 17, 35
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 5 cut(s) 314, 317, 515, 544, 682
PleI GAGTC 3 cut(s) 199, 358, 448
PpsI GAGTC 3 cut(s) 199, 358, 448
PscI ACATGT 2 cut(s) 17, 35
Psp6I CCWGG 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 276
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 4 cut(s) 62, 85, 560, 708
RsaNI GTAC 4 cut(s) 61, 84, 559, 707
RseI CAYNNNNRTG 1 cut(s) 497
SacII CCGCGG 1 cut(s) 316
SaqAI TTAA 3 cut(s) 210, 357, 414
SatI GCNGC 5 cut(s) 313, 316, 514, 543, 681
Sau3AI GATC 3 cut(s) 40, 240, 646
SchI GAGTC 3 cut(s) 200, 359, 449
ScrFI CCNGG 1 cut(s) 272
SfaNI GCATC 3 cut(s) 14, 234, 397
Sfr303I CCGCGG 1 cut(s) 316
SfuI TTCGAA 1 cut(s) 452
SgrBI CCGCGG 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 497
Sse9I AATT 7 cut(s) 70, 109, 121, 165, 265, 411, 416
SsiI CCGC 3 cut(s) 313, 315, 542
SspI AATATT 2 cut(s) 299, 400
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 560
TaqI TCGA 2 cut(s) 228, 452
TasI AATT 7 cut(s) 70, 109, 121, 165, 265, 411, 416
TatI WGTACW 1 cut(s) 60
TauI GCSGC 3 cut(s) 315, 318, 545
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 3 cut(s) 210, 357, 414
Tru9I TTAA 3 cut(s) 210, 357, 414
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 3 cut(s) 199, 217, 697
TseI GCWGC 2 cut(s) 513, 680
Tsp45I GTSAC 3 cut(s) 199, 217, 697
TspDTI ATGAA 4 cut(s) 141, 351, 496, 509
TspRI CASTG 1 cut(s) 675
XapI RAATTY 2 cut(s) 165, 416
XceI RCATGY 3 cut(s) 21, 39, 225
XcmI CCANNNNNNNNNTGG 1 cut(s) 371
Zsp2I ATGCAT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.