RLG00000011418

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017257
Physical Location & Seq
Reverse (-)
9295515 .. 9297890
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011418

Sequence Viewer

Length: 1011 bp
ATGGCAAAACAGAGCTCCAAGGGTCTTCCTTCGACATTCTGCCACAAACATATAGGAACAAGCGACCAGACGATTATATTGGAAGATGAAACTGGCGATAAGTTCAAAACAACATTTCTCGGTCATAAGATGGGACTGAGTGCTGGATGGAGAGGGTTTTCCATTCATCACCATTTGCTTGAGGGAGATGTTTTAGTCTTCCAAAAAGTCAGGGCTCATGAATTTAAGGTTTATATAGTTAAACCAAATGGTTCAAAAAAACTGAAGTTTCCCGATCACACAGAGGACTCTCAAATGGCATCCGTTACTACATGTAGTATTCCAACAATATCTGATCTTTCTGAAGATATATTGTACATTGACAATTATGTCGGAAGTACAGAGAGTGAAGAAGATATGTTCAATTATATTGACAATTATGAAGGCGGTGCAGAGAGTGAAGATGATATTGATCCTGAATTTTTCAAAGGAGCTCGGCTTTCGGAGTCTTCTGTCACTTATTTTAAAGAAGTGACATGCATCGAGAAGTTCAAGATCATATTCAATGGCTGTAACATCAATTCCCAGGTTCCTAAACTCGCCCTGAAGAAATATTATGAGCTTTGCCGCGGCCAAAACTCATACCTGCATGAAAATCTTGTGGAGTCTCTTAACAGCCAACTGGTTGCTGGACTGATCTCTGAGACGGTCAATATTGCAGATGCAATTAAATTCTGCAAGATTACCACCGAGGAGTCTGAGTTTTCGACGTGGGACAAGACCTTGCAAGCCTGTGAGATGTTAGGCATGAATGTGGGCTTCATGCGGAGCCGAGCGAGCAAGCTTGCAAGCCTTGCGGCAGAATCAAAGACGTACAAAGACGCGAGACTTGCAAGAGAGCAAGAAGAAGCAGAGTTTCGGGTCAGGTTGGCTGACTTTTTGAAAGAAAAAGATGAAGCAGATCGGGAGGGGTCTGAGAAATCAGTGCAGGTTGCTACTAGTTTCAAACAAGTGACAAATGTACCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

337

Amino Acids

37.91

Weight (kDa)

5.27

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 6 - 80 9.6e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017257)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41030 FvH4_2g41030
rosa_chinensis RchiOBHm_Chr6g0299531
rosa_laevigata RLG00000011418
rosa_multiflora Rmu_sc0004064.1_g000007
rosa_roxburghii Rroxscaffold_7G00168580
rosa_samantha Rh6AG400900 Rh6AG401000 Rh6BG409500 Rh6CG414900 Rh6DG402000 Rh6DG402100
rosa_wichuraiana Rw6G035090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
Acc36I ACCTGC 2 cut(s) 633, 958
AccII CGCG 2 cut(s) 609, 863
AciI CCGC 5 cut(s) 426, 607, 609, 805, 836
AclWI GGATC 1 cut(s) 446
AcoI YGGCCR 1 cut(s) 610
AcsI RAATTY 3 cut(s) 221, 458, 710
AcuI CTGAAG 3 cut(s) 284, 363, 605
AfaI GTAC 4 cut(s) 356, 379, 854, 1002
AflIII ACRYGT 1 cut(s) 311
AgsI TTSAA 8 cut(s) 106, 255, 403, 466, 532, 544, 922, 985
AhlI ACTAGT 1 cut(s) 977
AjiI CACGTC 1 cut(s) 750
AjnI CCWGG 1 cut(s) 564
AluBI AGCT 4 cut(s) 15, 473, 601, 823
AluI AGCT 4 cut(s) 15, 473, 601, 823
Alw21I GWGCWC 2 cut(s) 17, 475
Alw26I GTCTC 3 cut(s) 651, 677, 859
AlwI GGATC 1 cut(s) 446
AoxI GGCC 1 cut(s) 610
ApoI RAATTY 3 cut(s) 221, 458, 710
AsuHPI GGTGA 1 cut(s) 161
BaeI ACNNNNGTAYC 1 cut(s) 984
BanII GRGCYC 3 cut(s) 17, 217, 475
BbsI GAAGAC 3 cut(s) 17, 190, 480
Bbv12I GWGCWC 2 cut(s) 17, 475
BccI CCATC 2 cut(s) 124, 141
BciT130I CCWGG 1 cut(s) 566
BcoDI GTCTC 3 cut(s) 651, 677, 859
BcuI ACTAGT 1 cut(s) 977
BfaI CTAG 1 cut(s) 978
BfuAI ACCTGC 2 cut(s) 633, 958
BisI GCNGC 3 cut(s) 607, 610, 837
BlsI GCNGC 3 cut(s) 608, 611, 838
Bme1390I CCNGG 1 cut(s) 566
BmgBI CACGTC 1 cut(s) 750
BmiI GGNNCC 2 cut(s) 570, 809
BmrFI CCNGG 1 cut(s) 566
BmsI GCATC 3 cut(s) 308, 528, 691
BpiI GAAGAC 3 cut(s) 17, 190, 480
BpuEI CTTGAG 1 cut(s) 200
BsaBI GATNNNNATC 1 cut(s) 450
BsaJI CCNNGG 5 cut(s) 18, 564, 607, 729, 1004
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bse1I ACTGG 2 cut(s) 97, 666
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 1 cut(s) 566
BseDI CCNNGG 5 cut(s) 18, 564, 607, 729, 1004
BseGI GGATG 2 cut(s) 152, 299
BseJI GATNNNNATC 1 cut(s) 450
BseMII CTCAG 4 cut(s) 128, 672, 729, 945
BseNI ACTGG 2 cut(s) 97, 666
BseRI GAGGAG 1 cut(s) 746
BsgI GTGCAG 2 cut(s) 450, 986
Bsh1236I CGCG 2 cut(s) 609, 863
BshFI GGCC 1 cut(s) 612
BsiHKAI GWGCWC 2 cut(s) 17, 475
BslFI GGGAC 2 cut(s) 147, 767
BsmAI GTCTC 3 cut(s) 651, 677, 859
BsmBI CGTCTC 1 cut(s) 677
BsmFI GGGAC 2 cut(s) 147, 767
BsnI GGCC 1 cut(s) 612
Bsp1286I GDGCHC 3 cut(s) 17, 217, 475
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 6 cut(s) 274, 334, 451, 534, 675, 940
BspACI CCGC 5 cut(s) 426, 607, 609, 805, 836
BspANI GGCC 1 cut(s) 612
BspCNI CTCAG 4 cut(s) 129, 673, 730, 946
BspFNI CGCG 2 cut(s) 609, 863
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 2 cut(s) 570, 809
BspMI ACCTGC 2 cut(s) 633, 958
BspPI GGATC 1 cut(s) 446
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 2 cut(s) 97, 666
BssECI CCNNGG 5 cut(s) 18, 564, 607, 729, 1004
BssMI GATC 6 cut(s) 274, 334, 451, 534, 675, 940
BssT1I CCWWGG 2 cut(s) 18, 1004
Bst2UI CCWGG 1 cut(s) 566
Bst4CI ACNGT 1 cut(s) 688
BstAPI GCANNNNNTGC 1 cut(s) 833
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 5 cut(s) 768, 817, 821, 825, 829
BstDEI CTNAG 4 cut(s) 137, 681, 738, 954
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 2 cut(s) 152, 299
BstFNI CGCG 2 cut(s) 609, 863
BstKTI GATC 6 cut(s) 277, 337, 454, 537, 678, 943
BstMAI GTCTC 3 cut(s) 651, 677, 859
BstMBI GATC 6 cut(s) 274, 334, 451, 534, 675, 940
BstMWI GCNNNNNNNGC 3 cut(s) 816, 833, 869
BstNI CCWGG 1 cut(s) 566
BstNSI RCATGY 2 cut(s) 315, 519
BstSCI CCNGG 1 cut(s) 564
BstUI CGCG 2 cut(s) 609, 863
BstV2I GAAGAC 3 cut(s) 17, 190, 480
BsuRI GGCC 1 cut(s) 612
BtgI CCRYGG 1 cut(s) 607
BtrI CACGTC 1 cut(s) 750
BtsCI GGATG 2 cut(s) 152, 299
BtsIMutI CAGTG 1 cut(s) 969
BveI ACCTGC 2 cut(s) 633, 958
Cac8I GCNNGC 5 cut(s) 768, 817, 821, 825, 829
CciI TCATGA 1 cut(s) 217
Cfr42I CCGCGG 1 cut(s) 610
CseI GACGC 1 cut(s) 869
Csp6I GTAC 4 cut(s) 355, 378, 853, 1001
CviAII CATG 6 cut(s) 218, 312, 516, 629, 787, 802
CviQI GTAC 4 cut(s) 355, 378, 853, 1001
DdeI CTNAG 4 cut(s) 137, 681, 738, 954
DpnI GATC 6 cut(s) 276, 336, 453, 536, 677, 942
DpnII GATC 6 cut(s) 274, 334, 451, 534, 675, 940
DraI TTTAAA 1 cut(s) 505
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 610
Ecl136II GAGCTC 2 cut(s) 15, 473
Eco130I CCWWGG 2 cut(s) 18, 1004
Eco24I GRGCYC 3 cut(s) 17, 217, 475
Eco53kI GAGCTC 2 cut(s) 15, 473
Eco57I CTGAAG 3 cut(s) 284, 363, 605
EcoICRI GAGCTC 2 cut(s) 15, 473
EcoRII CCWGG 1 cut(s) 564
EcoT14I CCWWGG 2 cut(s) 18, 1004
EcoT22I ATGCAT 1 cut(s) 521
EcoT38I GRGCYC 3 cut(s) 17, 217, 475
ErhI CCWWGG 2 cut(s) 18, 1004
Esp3I CGTCTC 1 cut(s) 677
FaeI CATG 6 cut(s) 221, 315, 519, 632, 790, 805
FaqI GGGAC 2 cut(s) 147, 767
FatI CATG 6 cut(s) 217, 311, 515, 628, 786, 801
Fnu4HI GCNGC 3 cut(s) 607, 610, 837
FokI GGATG 2 cut(s) 159, 286
FriOI GRGCYC 3 cut(s) 17, 217, 475
Fsp4HI GCNGC 3 cut(s) 607, 610, 837
FspBI CTAG 1 cut(s) 978
GluI GCNGC 3 cut(s) 607, 610, 837
HaeIII GGCC 1 cut(s) 612
HgaI GACGC 1 cut(s) 869
Hin1II CATG 6 cut(s) 221, 315, 519, 632, 790, 805
HindIII AAGCTT 1 cut(s) 821
HinfI GANTC 5 cut(s) 287, 485, 644, 734, 842
HphI GGTGA 1 cut(s) 161
Hpy188I TCNGA 7 cut(s) 334, 343, 374, 484, 682, 739, 955
Hpy188III TCNNGA 6 cut(s) 218, 272, 455, 523, 532, 944
Hpy99I CGWCG 1 cut(s) 751
HpyAV CCTTC 2 cut(s) 39, 416
HpyCH4III ACNGT 1 cut(s) 688
HpyCH4IV ACGT 2 cut(s) 749, 851
HpyF10VI GCNNNNNNNGC 3 cut(s) 816, 833, 869
HpyF3I CTNAG 4 cut(s) 137, 681, 738, 954
HpySE526I ACGT 2 cut(s) 749, 851
Hsp92II CATG 6 cut(s) 221, 315, 519, 632, 790, 805
KspI CCGCGG 1 cut(s) 610
Kzo9I GATC 6 cut(s) 274, 334, 451, 534, 675, 940
LmnI GCTCC 3 cut(s) 20, 470, 807
LweI GCATC 3 cut(s) 308, 528, 691
MaeI CTAG 1 cut(s) 978
MaeII ACGT 2 cut(s) 749, 851
MaeIII GTNAC 5 cut(s) 304, 493, 511, 551, 991
MalI GATC 6 cut(s) 276, 336, 453, 536, 677, 942
MboI GATC 6 cut(s) 274, 334, 451, 534, 675, 940
MhlI GDGCHC 3 cut(s) 17, 217, 475
MluCI AATT 8 cut(s) 221, 364, 403, 415, 458, 559, 705, 710
MlyI GAGTC 4 cut(s) 281, 494, 653, 743
MmeI TCCRAC 2 cut(s) 347, 352
MnlI CCTC 5 cut(s) 146, 175, 277, 724, 940
Mph1103I ATGCAT 1 cut(s) 521
MseI TTAA 5 cut(s) 225, 240, 504, 651, 708
MslI CAYNNNNRTG 1 cut(s) 791
MspA1I CMGCKG 1 cut(s) 609
MspR9I CCNGG 1 cut(s) 566
MvaI CCWGG 1 cut(s) 566
MvnI CGCG 2 cut(s) 609, 863
MwoI GCNNNNNNNGC 3 cut(s) 816, 833, 869
NdeII GATC 6 cut(s) 274, 334, 451, 534, 675, 940
NlaIII CATG 6 cut(s) 221, 315, 519, 632, 790, 805
NlaIV GGNNCC 2 cut(s) 570, 809
NmeAIII GCCGAG 2 cut(s) 454, 836
NmuCI GTSAC 3 cut(s) 493, 511, 991
NsiI ATGCAT 1 cut(s) 521
NspI RCATGY 2 cut(s) 315, 519
PagI TCATGA 1 cut(s) 217
PciI ACATGT 1 cut(s) 311
PfeI GAWTC 1 cut(s) 842
PkrI GCNGC 3 cut(s) 608, 611, 838
PleI GAGTC 4 cut(s) 281, 493, 652, 742
PpsI GAGTC 4 cut(s) 281, 493, 652, 742
PscI ACATGT 1 cut(s) 311
Psp124BI GAGCTC 2 cut(s) 17, 475
Psp6I CCWGG 1 cut(s) 564
PspGI CCWGG 1 cut(s) 564
PspN4I GGNNCC 2 cut(s) 570, 809
RsaI GTAC 4 cut(s) 356, 379, 854, 1002
RsaNI GTAC 4 cut(s) 355, 378, 853, 1001
RseI CAYNNNNRTG 1 cut(s) 791
SacI GAGCTC 2 cut(s) 17, 475
SacII CCGCGG 1 cut(s) 610
SaqAI TTAA 5 cut(s) 225, 240, 504, 651, 708
SatI GCNGC 3 cut(s) 607, 610, 837
Sau3AI GATC 6 cut(s) 274, 334, 451, 534, 675, 940
SchI GAGTC 4 cut(s) 281, 494, 653, 743
ScrFI CCNGG 1 cut(s) 566
SduI GDGCHC 3 cut(s) 17, 217, 475
SfaNI GCATC 3 cut(s) 308, 528, 691
Sfr303I CCGCGG 1 cut(s) 610
SgrBI CCGCGG 1 cut(s) 610
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SpeI ACTAGT 1 cut(s) 977
Sse9I AATT 8 cut(s) 221, 364, 403, 415, 458, 559, 705, 710
SsiI CCGC 5 cut(s) 426, 607, 609, 805, 836
SspI AATATT 2 cut(s) 593, 694
SspMI CTAG 1 cut(s) 978
SstI GAGCTC 2 cut(s) 17, 475
StyD4I CCNGG 1 cut(s) 564
StyI CCWWGG 2 cut(s) 18, 1004
TaaI ACNGT 1 cut(s) 688
TaiI ACGT 2 cut(s) 752, 854
TaqI TCGA 3 cut(s) 32, 522, 746
TaqII GACCGA 1 cut(s) 110
TasI AATT 8 cut(s) 221, 364, 403, 415, 458, 559, 705, 710
TatI WGTACW 2 cut(s) 354, 377
TauI GCSGC 3 cut(s) 609, 612, 839
TfiI GAWTC 1 cut(s) 842
Tru1I TTAA 5 cut(s) 225, 240, 504, 651, 708
Tru9I TTAA 5 cut(s) 225, 240, 504, 651, 708
TscAI CASTG 1 cut(s) 969
TseFI GTSAC 3 cut(s) 493, 511, 991
Tsp45I GTSAC 3 cut(s) 493, 511, 991
TspDTI ATGAA 8 cut(s) 102, 155, 234, 435, 645, 790, 803, 948
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 1 cut(s) 969
XapI RAATTY 3 cut(s) 221, 458, 710
XceI RCATGY 2 cut(s) 315, 519
XcmI CCANNNNNNNNNTGG 1 cut(s) 665
XspI CTAG 1 cut(s) 978
Zsp2I ATGCAT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.