Rh6AG400900

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0017257
Physical Location & Seq
Forward (+)
59811047 .. 59845489
34443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG400900.1

Sequence Viewer

Length: 990 bp
ATGGATGGAAGTCCTTTCAATTTGAATTTATTTTTGATCATGCTTGCGGAATTATTATACTCCCTGTGTGGCGGGGCTTGCTCTGCAGATATTTCTATAGCGAGTTATAAAATTCAAAAATTCAAACAGGCTGCCGACCTCAAGAAAAATACTCTTGCAAGTTGTGCTGGTCAAACTCAGAGAGTCGTGACTGTAGCACAGATAAGGAACAATGATGCGCTCCACAAGTTTATTAGCTTAAGAAAAACGGGATCATCGATGCTCGGATCAACGTCTAAGCTCGTGAGACAACCATCCACACTAGCCGTTGAAATAGGAAACAAATTAACTATGATCCATCCATTGAAGGGTGTATTTTGCGGGCATCTTACATGGGAACGTGACACGATTAGATTATGGCGTCAAGTACGTTTGACACGACGATCACTACCACCTTCGAAGGCGCCCCCAATTGAGGCTAAGAAGATCGCTAAGAGGGAAAGACCTTCAAATAAACAAGAGAAACAGATACTTGAAAACAATGTGGCCTGTGGTAATTCAAAGAAGGCAGCTCAAAGGCCGAAACGATTAAAGAGAGCCATGTCTGAGAAATCATCACTTCTGGAAAGAGCTGAGGAACTTGCAGATCAAATGCCAGTAAAATGTCCTCATTTTGTCAAAGTGATGCTTCCATCACATGTTGCTGGAGGCTTCTGGCTGGGTCTTCCTTCGACATTCTGCCACAAACATATAGGAACAAGCGACCAGACGATTATATTGGAAGATGAAACTGGCGATAAGTTCAAAACAACATATCTCGGTCATAAGATGGGACTGAGTGCTGGATGGAGAGGGTTTTCCATTCATCACCATTTGCTTGAGGGAGATGTTTTGGTCTTCCAAAAAGTCACGGCCAATGAATTTAAGGTAAATATATATGTGATTCAGTCCTTCAATTGCTTGTTTAGATCCATGGCTGTATATTGTTTGATTTTGAGAGTGACATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

329

Amino Acids

36.83

Weight (kDa)

9.84

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 218 - 307 6.7e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017257)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41030 FvH4_2g41030
rosa_chinensis RchiOBHm_Chr6g0299531
rosa_laevigata RLG00000011418
rosa_multiflora Rmu_sc0004064.1_g000007
rosa_roxburghii Rroxscaffold_7G00168580
rosa_samantha Rh6AG400900 Rh6AG401000 Rh6BG409500 Rh6CG414900 Rh6DG402000 Rh6DG402100
rosa_wichuraiana Rw6G035090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 108
AccB1I GGYRCC 1 cut(s) 442
AciI CCGC 3 cut(s) 47, 72, 360
AclWI GGATC 4 cut(s) 259, 274, 328, 942
AcoI YGGCCR 1 cut(s) 891
AcsI RAATTY 4 cut(s) 25, 111, 119, 899
AcyI GRCGYC 2 cut(s) 400, 443
AfaI GTAC 1 cut(s) 408
AfiI CCNNNNNNNGG 2 cut(s) 347, 454
AflII CTTAAG 1 cut(s) 238
AflIII ACRYGT 1 cut(s) 676
AluBI AGCT 4 cut(s) 237, 280, 551, 611
AluI AGCT 4 cut(s) 237, 280, 551, 611
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 4 cut(s) 259, 274, 328, 942
AoxI GGCC 3 cut(s) 525, 557, 891
ApeKI GCWGC 2 cut(s) 131, 548
ApoI RAATTY 4 cut(s) 25, 111, 119, 899
AspLEI GCGC 2 cut(s) 220, 445
AsuHPI GGTGA 1 cut(s) 839
AsuII TTCGAA 1 cut(s) 437
BanI GGYRCC 1 cut(s) 442
BauI CACGAG 1 cut(s) 281
BbsI GAAGAC 2 cut(s) 695, 868
BbvCI CCTCAGC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 118, 560
BccI CCATC 5 cut(s) 301, 345, 679, 802, 819
BceAI ACGGC 2 cut(s) 290, 906
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 3 cut(s) 84, 96, 192
BfoI RGCGCY 1 cut(s) 446
BfrI CTTAAG 1 cut(s) 238
BisI GCNGC 2 cut(s) 132, 549
BlsI GCNGC 2 cut(s) 133, 550
BmiI GGNNCC 1 cut(s) 444
BmsI GCATC 4 cut(s) 205, 249, 373, 654
BpiI GAAGAC 2 cut(s) 695, 868
BpmI CTGGAG 1 cut(s) 705
Bpu10I CCTNAGC 1 cut(s) 612
Bpu14I TTCGAA 1 cut(s) 437
BpuEI CTTGAG 2 cut(s) 125, 878
Bsa29I ATCGAT 1 cut(s) 257
BsaHI GRCGYC 2 cut(s) 400, 443
BsaJI CCNNGG 1 cut(s) 951
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 454
Bse1I ACTGG 2 cut(s) 635, 775
BseCI ATCGAT 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 951
BseGI GGATG 4 cut(s) 10, 293, 337, 830
BseLI CCNNNNNNNGG 2 cut(s) 347, 454
BseMII CTCAG 4 cut(s) 191, 576, 603, 806
BseNI ACTGG 2 cut(s) 635, 775
BseXI GCAGC 2 cut(s) 118, 560
BseYI CCCAGC 1 cut(s) 697
BshFI GGCC 3 cut(s) 527, 559, 893
BshNI GGYRCC 1 cut(s) 442
BshVI ATCGAT 1 cut(s) 257
BslFI GGGAC 1 cut(s) 825
BslI CCNNNNNNNGG 2 cut(s) 347, 454
BsmAI GTCTC 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 825
BsnI GGCC 3 cut(s) 527, 559, 893
Bsp119I TTCGAA 1 cut(s) 437
Bsp143I GATC 8 cut(s) 36, 251, 266, 333, 422, 465, 625, 947
Bsp19I CCATGG 1 cut(s) 951
BspACI CCGC 3 cut(s) 47, 72, 360
BspANI GGCC 3 cut(s) 527, 559, 893
BspCNI CTCAG 4 cut(s) 190, 577, 604, 807
BspDI ATCGAT 1 cut(s) 257
BspLI GGNNCC 1 cut(s) 444
BspMAI CTGCAG 1 cut(s) 88
BspPI GGATC 4 cut(s) 259, 274, 328, 942
BspT104I TTCGAA 1 cut(s) 437
BspT107I GGYRCC 1 cut(s) 442
BspTI CTTAAG 1 cut(s) 238
BsrI ACTGG 2 cut(s) 635, 775
BssECI CCNNGG 1 cut(s) 951
BssMI GATC 8 cut(s) 36, 251, 266, 333, 422, 465, 625, 947
BssNI GRCGYC 2 cut(s) 400, 443
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 951
Bst2BI CACGAG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 193
BstACI GRCGYC 2 cut(s) 400, 443
BstAFI CTTAAG 1 cut(s) 238
BstAPI GCANNNNNTGC 1 cut(s) 164
BstBI TTCGAA 1 cut(s) 437
BstC8I GCNNGC 3 cut(s) 45, 79, 362
BstDEI CTNAG 7 cut(s) 177, 276, 459, 471, 585, 612, 815
BstDSI CCRYGG 1 cut(s) 951
BstF5I GGATG 4 cut(s) 10, 293, 337, 830
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 2 cut(s) 220, 445
BstKTI GATC 8 cut(s) 39, 254, 269, 336, 425, 468, 628, 950
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 8 cut(s) 36, 251, 266, 333, 422, 465, 625, 947
BstMWI GCNNNNNNNGC 3 cut(s) 78, 83, 164
BstNSI RCATGY 1 cut(s) 680
BstSFI CTRYAG 3 cut(s) 84, 96, 192
BstV1I GCAGC 2 cut(s) 118, 560
BstV2I GAAGAC 2 cut(s) 695, 868
BstX2I RGATCY 1 cut(s) 947
BstYI RGATCY 1 cut(s) 947
Bsu15I ATCGAT 1 cut(s) 257
BsuRI GGCC 3 cut(s) 527, 559, 893
BsuTUI ATCGAT 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 951
BtsCI GGATG 4 cut(s) 10, 293, 337, 830
Cac8I GCNNGC 3 cut(s) 45, 79, 362
CfoI GCGC 2 cut(s) 220, 445
ClaI ATCGAT 1 cut(s) 257
CseI GACGC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 407
CviAII CATG 5 cut(s) 40, 372, 580, 677, 952
CviQI GTAC 1 cut(s) 407
DdeI CTNAG 7 cut(s) 177, 276, 459, 471, 585, 612, 815
DinI GGCGCC 1 cut(s) 444
DpnI GATC 8 cut(s) 38, 253, 268, 335, 424, 467, 627, 949
DpnII GATC 8 cut(s) 36, 251, 266, 333, 422, 465, 625, 947
EaeI YGGCCR 1 cut(s) 891
Eco130I CCWWGG 1 cut(s) 951
EcoT14I CCWWGG 1 cut(s) 951
EgeI GGCGCC 1 cut(s) 444
EheI GGCGCC 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 951
FaeI CATG 5 cut(s) 43, 375, 583, 680, 955
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FaqI GGGAC 1 cut(s) 825
FatI CATG 5 cut(s) 39, 371, 579, 676, 951
FauI CCCGC 2 cut(s) 65, 353
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 132, 549
FokI GGATG 4 cut(s) 17, 280, 324, 837
Fsp4HI GCNGC 2 cut(s) 132, 549
FspBI CTAG 1 cut(s) 302
GlaI GCGC 2 cut(s) 219, 444
GluI GCNGC 2 cut(s) 132, 549
GsaI CCCAGC 1 cut(s) 701
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 3 cut(s) 527, 559, 893
HgaI GACGC 1 cut(s) 389
HhaI GCGC 2 cut(s) 220, 445
Hin1I GRCGYC 2 cut(s) 400, 443
Hin1II CATG 5 cut(s) 43, 375, 583, 680, 955
Hin6I GCGC 2 cut(s) 218, 443
HinP1I GCGC 2 cut(s) 218, 443
HinfI GANTC 2 cut(s) 183, 922
HphI GGTGA 1 cut(s) 839
Hpy188I TCNGA 3 cut(s) 180, 266, 586
Hpy188III TCNNGA 4 cut(s) 142, 187, 283, 602
Hpy99I CGWCG 1 cut(s) 423
HpyAV CCTTC 7 cut(s) 340, 433, 444, 495, 538, 717, 940
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 3 cut(s) 272, 379, 409
HpyCH4V TGCA 3 cut(s) 86, 158, 623
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 83, 164
HpyF3I CTNAG 7 cut(s) 177, 276, 459, 471, 585, 612, 815
HpySE526I ACGT 3 cut(s) 272, 379, 409
Hsp92I GRCGYC 2 cut(s) 400, 443
Hsp92II CATG 5 cut(s) 43, 375, 583, 680, 955
HspAI GCGC 2 cut(s) 218, 443
KasI GGCGCC 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 8 cut(s) 36, 251, 266, 333, 422, 465, 625, 947
LmnI GCTCC 1 cut(s) 225
Lsp1109I GCAGC 2 cut(s) 118, 560
LweI GCATC 4 cut(s) 205, 249, 373, 654
MaeI CTAG 1 cut(s) 302
MaeII ACGT 3 cut(s) 272, 379, 409
MaeIII GTNAC 4 cut(s) 187, 380, 886, 979
MalI GATC 8 cut(s) 38, 253, 268, 335, 424, 467, 627, 949
MboI GATC 8 cut(s) 36, 251, 266, 333, 422, 465, 625, 947
MboII GAAGA 4 cut(s) 475, 695, 773, 868
MfeI CAATTG 2 cut(s) 450, 934
MflI RGATCY 1 cut(s) 947
Mly113I GGCGCC 1 cut(s) 443
MlyI GAGTC 1 cut(s) 192
MnlI CCTC 8 cut(s) 149, 448, 468, 607, 657, 680, 824, 853
MseI TTAA 4 cut(s) 239, 326, 569, 903
MspCI CTTAAG 1 cut(s) 238
MunI CAATTG 2 cut(s) 450, 934
MwoI GCNNNNNNNGC 3 cut(s) 78, 83, 164
NarI GGCGCC 1 cut(s) 443
NcoI CCATGG 1 cut(s) 951
NdeII GATC 8 cut(s) 36, 251, 266, 333, 422, 465, 625, 947
NlaIII CATG 5 cut(s) 43, 375, 583, 680, 955
NlaIV GGNNCC 1 cut(s) 444
NmuCI GTSAC 4 cut(s) 187, 380, 886, 979
NspI RCATGY 1 cut(s) 680
NspV TTCGAA 1 cut(s) 437
PciI ACATGT 1 cut(s) 676
PcsI WCGNNNNNNNCGW 2 cut(s) 254, 415
PfeI GAWTC 1 cut(s) 922
PkrI GCNGC 2 cut(s) 133, 550
PleI GAGTC 1 cut(s) 191
PluTI GGCGCC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 191
PscI ACATGT 1 cut(s) 676
PsiI TTATAA 1 cut(s) 108
PspFI CCCAGC 1 cut(s) 697
PspN4I GGNNCC 1 cut(s) 444
PstI CTGCAG 1 cut(s) 88
PsuI RGATCY 1 cut(s) 947
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
SaqAI TTAA 4 cut(s) 239, 326, 569, 903
SatI GCNGC 2 cut(s) 132, 549
Sau3AI GATC 8 cut(s) 36, 251, 266, 333, 422, 465, 625, 947
SchI GAGTC 1 cut(s) 192
SfaNI GCATC 4 cut(s) 205, 249, 373, 654
SfcI CTRYAG 3 cut(s) 84, 96, 192
SfoI GGCGCC 1 cut(s) 444
SfuI TTCGAA 1 cut(s) 437
SmlI CTYRAG 3 cut(s) 140, 238, 857
SmoI CTYRAG 3 cut(s) 140, 238, 857
SsiI CCGC 3 cut(s) 47, 72, 360
SspDI GGCGCC 1 cut(s) 442
SspMI CTAG 1 cut(s) 302
StyI CCWWGG 1 cut(s) 951
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 3 cut(s) 275, 382, 412
TaqI TCGA 3 cut(s) 257, 437, 710
TaqII GACCGA 1 cut(s) 788
TfiI GAWTC 1 cut(s) 922
Tru1I TTAA 4 cut(s) 239, 326, 569, 903
Tru9I TTAA 4 cut(s) 239, 326, 569, 903
TseFI GTSAC 4 cut(s) 187, 380, 886, 979
TseI GCWGC 2 cut(s) 131, 548
Tsp45I GTSAC 4 cut(s) 187, 380, 886, 979
TspDTI ATGAA 3 cut(s) 780, 833, 912
Vha464I CTTAAG 1 cut(s) 238
XapI RAATTY 4 cut(s) 25, 111, 119, 899
XceI RCATGY 1 cut(s) 680
XspI CTAG 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.