Rroxscaffold_7G00168580

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017257
Physical Location & Seq
Reverse (-)
9434169 .. 9436522
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00168580.1

Sequence Viewer

Length: 1191 bp
ATGGCAAAACAGAGCTCCAAGGTGGCCTGTGGTAATTCAAAGAAGGCAGCTCAAAGGCCGAAACGATTAAAGAGAGCCGTATCTGAGAAATCATCACTTCTGGAAAGAGCTGAGGAACTTGCAGATCAAATGCCAGTAAAATGTCCTCATTTTGTCAAAGTGATGCTTCCATCACATGTTGCTGGAGGCTTCTGGCTGGGTCTTCCTTCGACATTCTGCCACAAACATATAGGAACAAGCGACCAGAAGATTATATTGGAAGATGAAACTGGCGATAAGTTCGAAACAACATATCTCGGTCATAAGATGGGACTAAGTGCTGGATGGAGAGGGTTTTCCATTCATCACCATTTGCTTGAGGGAGATGTTTTAGTCTTCCAAAAGTCAGGGCTCATGAATTTAAGGGTTTATATAGTTAAACCAAATGGTTCAAAAAAACTGAAGTTTCCCGATCACGCAGAGGACTCTCAAATGGCATCCGTTACTACATGTAGTATTCCAACAATATCTGATCTTTCTGAAGATATATTGTACATTGACAATTATGTCGGAAGTACAGAGAGTGAAGAAGATATGTTCAATTATATTGACAATTATGAAGCCGGTGCAGAGAGTAATGAAGATGATATTGACCCTGAATTTTTCAAAGGAGCTGGGCTTTCGGAGTCTTCTGTCACTTCTTTTAAAGAAGTGACATGCATCGAGAAGTTCAAGATCATATTCAATGGCTGTAACATCAATTCCCAGGTTCCTAAACTCGTCCTGAAGAAATATTATGAGCTTTGCCGCGGCCAAAACTCATACCTGCATGAAAATCTTGTGGAGTCTCTTAACAGACAACTGGTTGCTGGACTAATCTCTGAGACGGTCAATATTGCAGATGCAATTAAATTCTGCAAGATTACCACCGAGGAGTCTGAGTTTTCGACGTGGGACAAGACCTTGCAAGCCTCTGAGATGTTAGGCATGAGTGTGGGCTTCATGCGCAGCCGAGCGAGCAAGCTTGCAAGCCTTGCGGCAGAATCAAAGACGTACAAAGACGCGAGACTTGCAAGAGAGCAAGAAGAAGCAGAGTTCCGGGTCAGGTTGGCTGACTTTTTGAAAGAAAAAGATAAAGCAGATCGGGAGGGGTCTGAGAAATCAGTGCAGGTTGCTACAAGTTTCAGACAAGTGACAAATGTACCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

396

Amino Acids

44.38

Weight (kDa)

6.0

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 51 - 138 8.8e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017257)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g41030 FvH4_2g41030
rosa_chinensis RchiOBHm_Chr6g0299531
rosa_laevigata RLG00000011418
rosa_multiflora Rmu_sc0004064.1_g000007
rosa_roxburghii Rroxscaffold_7G00168580
rosa_samantha Rh6AG400900 Rh6AG401000 Rh6BG409500 Rh6CG414900 Rh6DG402000 Rh6DG402100
rosa_wichuraiana Rw6G035090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 545
Acc16I TGCGCA 1 cut(s) 986
Acc36I ACCTGC 2 cut(s) 813, 1138
AccII CGCG 2 cut(s) 789, 1043
AciI CCGC 3 cut(s) 787, 789, 1016
AcoI YGGCCR 1 cut(s) 790
AcsI RAATTY 3 cut(s) 397, 638, 890
AcuI CTGAAG 3 cut(s) 461, 540, 785
AfaI GTAC 4 cut(s) 533, 556, 1034, 1182
AflIII ACRYGT 2 cut(s) 175, 488
AgsI TTSAA 7 cut(s) 39, 432, 580, 646, 712, 724, 1102
AjiI CACGTC 1 cut(s) 930
AjnI CCWGG 1 cut(s) 744
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AluBI AGCT 6 cut(s) 15, 50, 110, 653, 781, 1003
AluI AGCT 6 cut(s) 15, 50, 110, 653, 781, 1003
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 3 cut(s) 831, 857, 1039
AoxI GGCC 3 cut(s) 24, 56, 790
ApeKI GCWGC 2 cut(s) 47, 987
ApoI RAATTY 3 cut(s) 397, 638, 890
AspLEI GCGC 1 cut(s) 987
AsuC2I CCSGG 1 cut(s) 1079
AsuHPI GGTGA 1 cut(s) 338
AsuII TTCGAA 1 cut(s) 282
BaeI ACNNNNGTAYC 1 cut(s) 1164
BanII GRGCYC 2 cut(s) 17, 393
BbsI GAAGAC 3 cut(s) 194, 367, 660
Bbv12I GWGCWC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 59, 999
BccI CCATC 3 cut(s) 178, 301, 318
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 746
BcnI CCSGG 1 cut(s) 1079
BcoDI GTCTC 3 cut(s) 831, 857, 1039
BfuAI ACCTGC 2 cut(s) 813, 1138
BisI GCNGC 5 cut(s) 48, 787, 790, 988, 1017
BlsI GCNGC 5 cut(s) 49, 788, 791, 989, 1018
Bme1390I CCNGG 2 cut(s) 746, 1079
BmgBI CACGTC 1 cut(s) 930
BmiI GGNNCC 1 cut(s) 750
BmrFI CCNGG 2 cut(s) 746, 1079
BmsI GCATC 4 cut(s) 153, 485, 708, 871
BpiI GAAGAC 3 cut(s) 194, 367, 660
BpmI CTGGAG 1 cut(s) 204
Bpu10I CCTNAGC 1 cut(s) 111
Bpu14I TTCGAA 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 377
BpuMI CCSGG 1 cut(s) 1079
BsaJI CCNNGG 5 cut(s) 18, 744, 787, 909, 1184
BsaXI ACNNNNNCTCC 2 cut(s) 905, 935
Bse118I RCCGGY 1 cut(s) 602
Bse1I ACTGG 3 cut(s) 134, 274, 846
BseBI CCWGG 1 cut(s) 746
BseDI CCNNGG 5 cut(s) 18, 744, 787, 909, 1184
BseGI GGATG 2 cut(s) 329, 476
BseMII CTCAG 6 cut(s) 75, 102, 852, 909, 945, 1125
BseNI ACTGG 3 cut(s) 134, 274, 846
BseRI GAGGAG 1 cut(s) 926
BseXI GCAGC 2 cut(s) 59, 999
BseYI CCCAGC 2 cut(s) 196, 653
BsgI GTGCAG 2 cut(s) 627, 1166
Bsh1236I CGCG 2 cut(s) 789, 1043
BshFI GGCC 3 cut(s) 26, 58, 792
BsiHKAI GWGCWC 1 cut(s) 17
BsiSI CCGG 2 cut(s) 603, 1078
BslFI GGGAC 2 cut(s) 324, 947
BsmAI GTCTC 3 cut(s) 831, 857, 1039
BsmBI CGTCTC 1 cut(s) 857
BsmFI GGGAC 2 cut(s) 324, 947
BsnI GGCC 3 cut(s) 26, 58, 792
Bsp119I TTCGAA 1 cut(s) 282
Bsp1286I GDGCHC 2 cut(s) 17, 393
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 5 cut(s) 124, 451, 511, 714, 1120
BspACI CCGC 3 cut(s) 787, 789, 1016
BspANI GGCC 3 cut(s) 26, 58, 792
BspCNI CTCAG 6 cut(s) 76, 103, 853, 910, 946, 1126
BspFNI CGCG 2 cut(s) 789, 1043
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 1 cut(s) 750
BspMI ACCTGC 2 cut(s) 813, 1138
BspT104I TTCGAA 1 cut(s) 282
BsrFI RCCGGY 1 cut(s) 602
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 3 cut(s) 134, 274, 846
BssAI RCCGGY 1 cut(s) 602
BssECI CCNNGG 5 cut(s) 18, 744, 787, 909, 1184
BssMI GATC 5 cut(s) 124, 451, 511, 714, 1120
BssT1I CCWWGG 2 cut(s) 18, 1184
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 1 cut(s) 868
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 531
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 5 cut(s) 948, 997, 1001, 1005, 1009
BstDEI CTNAG 7 cut(s) 84, 111, 314, 861, 918, 954, 1134
BstDSI CCRYGG 1 cut(s) 787
BstF5I GGATG 2 cut(s) 329, 476
BstFNI CGCG 2 cut(s) 789, 1043
BstHHI GCGC 1 cut(s) 987
BstKTI GATC 5 cut(s) 127, 454, 514, 717, 1123
BstMAI GTCTC 3 cut(s) 831, 857, 1039
BstMBI GATC 5 cut(s) 124, 451, 511, 714, 1120
BstMWI GCNNNNNNNGC 4 cut(s) 984, 996, 1013, 1049
BstNI CCWGG 1 cut(s) 746
BstNSI RCATGY 3 cut(s) 179, 492, 699
BstSCI CCNGG 2 cut(s) 744, 1077
BstUI CGCG 2 cut(s) 789, 1043
BstV1I GCAGC 2 cut(s) 59, 999
BstV2I GAAGAC 3 cut(s) 194, 367, 660
BsuRI GGCC 3 cut(s) 26, 58, 792
BtgI CCRYGG 1 cut(s) 787
BtrI CACGTC 1 cut(s) 930
BtsCI GGATG 2 cut(s) 329, 476
BtsIMutI CAGTG 1 cut(s) 1149
BveI ACCTGC 2 cut(s) 813, 1138
Cac8I GCNNGC 5 cut(s) 948, 997, 1001, 1005, 1009
CciI TCATGA 1 cut(s) 393
CfoI GCGC 1 cut(s) 987
Cfr10I RCCGGY 1 cut(s) 602
Cfr42I CCGCGG 1 cut(s) 790
CseI GACGC 1 cut(s) 1049
Csp6I GTAC 4 cut(s) 532, 555, 1033, 1181
CviAII CATG 7 cut(s) 176, 394, 489, 696, 809, 967, 982
CviQI GTAC 4 cut(s) 532, 555, 1033, 1181
DdeI CTNAG 7 cut(s) 84, 111, 314, 861, 918, 954, 1134
DpnI GATC 5 cut(s) 126, 453, 513, 716, 1122
DpnII GATC 5 cut(s) 124, 451, 511, 714, 1120
DraI TTTAAA 1 cut(s) 685
DrdI GACNNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 545
EaeI YGGCCR 1 cut(s) 790
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 2 cut(s) 18, 1184
Eco24I GRGCYC 2 cut(s) 17, 393
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 3 cut(s) 461, 540, 785
EcoICRI GAGCTC 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 744
EcoT14I CCWWGG 2 cut(s) 18, 1184
EcoT22I ATGCAT 1 cut(s) 701
EcoT38I GRGCYC 2 cut(s) 17, 393
ErhI CCWWGG 2 cut(s) 18, 1184
Esp3I CGTCTC 1 cut(s) 857
FaeI CATG 7 cut(s) 179, 397, 492, 699, 812, 970, 985
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 2 cut(s) 324, 947
FatI CATG 7 cut(s) 175, 393, 488, 695, 808, 966, 981
Fnu4HI GCNGC 5 cut(s) 48, 787, 790, 988, 1017
FokI GGATG 2 cut(s) 336, 463
FriOI GRGCYC 2 cut(s) 17, 393
Fsp4HI GCNGC 5 cut(s) 48, 787, 790, 988, 1017
FspI TGCGCA 1 cut(s) 986
GlaI GCGC 1 cut(s) 986
GluI GCNGC 5 cut(s) 48, 787, 790, 988, 1017
GsaI CCCAGC 2 cut(s) 200, 657
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 3 cut(s) 26, 58, 792
HapII CCGG 2 cut(s) 603, 1078
HgaI GACGC 1 cut(s) 1049
HhaI GCGC 1 cut(s) 987
Hin1II CATG 7 cut(s) 179, 397, 492, 699, 812, 970, 985
Hin6I GCGC 1 cut(s) 985
HinP1I GCGC 1 cut(s) 985
HindIII AAGCTT 1 cut(s) 1001
HinfI GANTC 5 cut(s) 464, 665, 824, 914, 1022
HpaII CCGG 2 cut(s) 603, 1078
HphI GGTGA 1 cut(s) 338
Hpy188III TCNNGA 7 cut(s) 101, 394, 449, 703, 712, 763, 1124
Hpy99I CGWCG 1 cut(s) 931
HpyAV CCTTC 2 cut(s) 37, 216
HpyCH4III ACNGT 1 cut(s) 868
HpyCH4IV ACGT 2 cut(s) 929, 1031
HpyF10VI GCNNNNNNNGC 4 cut(s) 984, 996, 1013, 1049
HpyF3I CTNAG 7 cut(s) 84, 111, 314, 861, 918, 954, 1134
HpySE526I ACGT 2 cut(s) 929, 1031
Hsp92II CATG 7 cut(s) 179, 397, 492, 699, 812, 970, 985
HspAI GCGC 1 cut(s) 985
KspI CCGCGG 1 cut(s) 790
Kzo9I GATC 5 cut(s) 124, 451, 511, 714, 1120
LmnI GCTCC 2 cut(s) 20, 650
Lsp1109I GCAGC 2 cut(s) 59, 999
LweI GCATC 4 cut(s) 153, 485, 708, 871
MaeII ACGT 2 cut(s) 929, 1031
MaeIII GTNAC 5 cut(s) 481, 673, 691, 731, 1171
MalI GATC 5 cut(s) 126, 453, 513, 716, 1122
MboI GATC 5 cut(s) 124, 451, 511, 714, 1120
MhlI GDGCHC 2 cut(s) 17, 393
MluCI AATT 9 cut(s) 34, 397, 541, 580, 592, 638, 739, 885, 890
MlyI GAGTC 4 cut(s) 458, 674, 833, 923
MmeI TCCRAC 2 cut(s) 524, 529
MnlI CCTC 9 cut(s) 106, 156, 179, 323, 352, 454, 904, 961, 1120
Mph1103I ATGCAT 1 cut(s) 701
MseI TTAA 6 cut(s) 68, 401, 417, 684, 831, 888
MslI CAYNNNNRTG 1 cut(s) 971
MspA1I CMGCKG 1 cut(s) 789
MspI CCGG 2 cut(s) 603, 1078
MspR9I CCNGG 2 cut(s) 746, 1079
MvaI CCWGG 1 cut(s) 746
MvnI CGCG 2 cut(s) 789, 1043
MwoI GCNNNNNNNGC 4 cut(s) 984, 996, 1013, 1049
NciI CCSGG 1 cut(s) 1079
NdeII GATC 5 cut(s) 124, 451, 511, 714, 1120
NlaIII CATG 7 cut(s) 179, 397, 492, 699, 812, 970, 985
NlaIV GGNNCC 1 cut(s) 750
NmeAIII GCCGAG 1 cut(s) 1016
NmuCI GTSAC 3 cut(s) 673, 691, 1171
NsbI TGCGCA 1 cut(s) 986
NsiI ATGCAT 1 cut(s) 701
NspI RCATGY 3 cut(s) 179, 492, 699
NspV TTCGAA 1 cut(s) 282
PagI TCATGA 1 cut(s) 393
PciI ACATGT 2 cut(s) 175, 488
PfeI GAWTC 1 cut(s) 1022
PkrI GCNGC 5 cut(s) 49, 788, 791, 989, 1018
PleI GAGTC 4 cut(s) 458, 673, 832, 922
PpsI GAGTC 4 cut(s) 458, 673, 832, 922
PscI ACATGT 2 cut(s) 175, 488
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 744
PspFI CCCAGC 2 cut(s) 196, 653
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 1 cut(s) 750
RsaI GTAC 4 cut(s) 533, 556, 1034, 1182
RsaNI GTAC 4 cut(s) 532, 555, 1033, 1181
RseI CAYNNNNRTG 1 cut(s) 971
SacI GAGCTC 1 cut(s) 17
SacII CCGCGG 1 cut(s) 790
SaqAI TTAA 6 cut(s) 68, 401, 417, 684, 831, 888
SatI GCNGC 5 cut(s) 48, 787, 790, 988, 1017
Sau3AI GATC 5 cut(s) 124, 451, 511, 714, 1120
SchI GAGTC 4 cut(s) 458, 674, 833, 923
ScrFI CCNGG 2 cut(s) 746, 1079
SduI GDGCHC 2 cut(s) 17, 393
SfaNI GCATC 4 cut(s) 153, 485, 708, 871
Sfr303I CCGCGG 1 cut(s) 790
SfuI TTCGAA 1 cut(s) 282
SgrBI CCGCGG 1 cut(s) 790
SmiMI CAYNNNNRTG 1 cut(s) 971
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 9 cut(s) 34, 397, 541, 580, 592, 638, 739, 885, 890
SsiI CCGC 3 cut(s) 787, 789, 1016
SspI AATATT 2 cut(s) 773, 874
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 744, 1077
StyI CCWWGG 2 cut(s) 18, 1184
TaaI ACNGT 1 cut(s) 868
TaiI ACGT 2 cut(s) 932, 1034
TaqI TCGA 4 cut(s) 209, 282, 702, 926
TaqII GACCGA 1 cut(s) 287
TasI AATT 9 cut(s) 34, 397, 541, 580, 592, 638, 739, 885, 890
TatI WGTACW 2 cut(s) 531, 554
TauI GCSGC 3 cut(s) 789, 792, 1019
TfiI GAWTC 1 cut(s) 1022
Tru1I TTAA 6 cut(s) 68, 401, 417, 684, 831, 888
Tru9I TTAA 6 cut(s) 68, 401, 417, 684, 831, 888
TscAI CASTG 1 cut(s) 1149
TseFI GTSAC 3 cut(s) 673, 691, 1171
TseI GCWGC 2 cut(s) 47, 987
Tsp45I GTSAC 3 cut(s) 673, 691, 1171
TspDTI ATGAA 7 cut(s) 279, 332, 410, 612, 633, 825, 970
TspGWI ACGGA 1 cut(s) 469
TspRI CASTG 1 cut(s) 1149
XapI RAATTY 3 cut(s) 397, 638, 890
XceI RCATGY 3 cut(s) 179, 492, 699
Zsp2I ATGCAT 1 cut(s) 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.