FvH4_3g00310

Sister chromatid cohesion protein PDS5 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009376
Physical Location & Seq
Reverse (-)
175818 .. 187384
11567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00310.t1

Sequence Viewer

Length: 5019 bp
ATGGCGTCGCAAAAAGTGGAGCAGCAGCTCAAGGAGGTGGGATCCAAGCTCGACTCGTCTCCTTCCTCCAAAGACGCTCTCGTCAAGCTCCTCAAGCAAGCTGCATCATGTCTTTCTGAGTTGGATCAGTCTCCTCCCGCAACTACCTTGGAGTCAATGCAGCCTTTTCTTAATGCAATAGTCAAACCAGAATTGTTGAAGCATCAAGATAGAGATGTCAAGCTTCTAGTAGCAACATGTATCTGTGAGATAACCCGGATAACTGCACCAGAAGCGCCTTATAGTGATGATGTGCTAAAGGATGTTTTTCGACTGATTGTGGGCACTTTTAGTGGATTGAAAGATACAAGTGGTCCATCATTTGGTAGGAGAGTTGTCATCCTGGAGACTCTTGCAAAATATAGGTCATGTGTTGTAATGTTGGACCTCGAATGTGATGATCTGGTGACTGAAATGTTCAGTACGTTTTTTGCTGTTGCCAGAGATGATCACCATGAATCTGTACTCTCAGCAATGCAGACAATCATGATTGTTCTCTTAGAAGAGAGCGAGGATTTGCAAGACGATCTTCTATTTGTCATCTTGTCTGTGTTAGGTCGGAAGAGAAGTGATATCACTGTGGCTGCTAGGAGACTTGCTATGAATGTTATAGAACAGAGTGCGGGAAAGCTTGAATCGGGTATCAGACAGTTCCTTATTTCATCAATGTCAGGCGACAATAAGTCAACTGATCATCAGATCGACTATCATGAAGTTATCTATGATGTGTATCGTTCTGCTCCCCAAATTGTGTCAGCAGTTGTTCCTTACCTAACTGGAGAGCTACTGACTGATCAGCTGGACACTCGTTTAAAAGCAGTTAACTTGGTGGGAGACTTATTTTCACTGCCGGGCTCTACCATTTCGGAACCATTTCAGCCGATCTTTTCAGAGTTTTTGAAGAGGTTGACTGATAGAGTTGTTGAGGTTCGAATGTCGGTCCTTGAACATGTTAAGAGCTGTATGCTGTCAAATCCTTTCAGAGCTGAAGCTCCCGAAATAATCTCTGCTCTGTGTGACCGGCTGTTGGACTATGAAGAGAAAGTTCGGAAGCAAGTTGTTGCTGTAATTTATGATGTGGCATGTCATGACCTAAATTCTATTCCACTAGAAACAGTAAAACTTGTTGCCGAGCGCCTTCGAGATAAATCTGTACTTGTTAAAAAGTATACCATGGAGAGGCTAGCTGAGATATACAGGGTTTATTGTGCAAAGTGCTCCGATGGCTCAACCATTTCCAGTGAGTTTGAGTGGATTCCTGGGAAGATTCTGAGATGTATTTATGATAAAGATTTCAGATCAGACACAATAGAAAATGTTCTTTGTGAATCTCTATTCCCAACTGAATTCTCTATCAAAGATAAAGTAAAACATTGGGTGAGGGTCTTCTCAGTATTTGATAAAGTTGAGGTGAAGGCACTTGAGAAGATACTGGAGCAGAAGCAAAGGTTACTACAAGAGATGCAGAAGTACATGTCACTTAGGCAGGTGCATCAGGATGGTGATGCTCCTGAGATCCAAAAAAAGATATTGTTTTGTTTTCGCATAATGGCACGCTCGTTTGCTGACCCTGCAAAGGCTGAGGAAAATTTTCAGTTTCTTGATCAATTGAAGGATGCTAACATCTGGAAGATTTTAATGAATCTTGTTGATCCAAACACTAGCTTCCATCAAGCCCGAACTTTACGGGATGAATTGCTTAAAATTTTGGGTGAAAAACACCGACTATATGACTTTCTTAGTACTCTCTCTGTCAAGTGTTCTTATCTACTTTTCAACAAGGAGCATGTGAAGGAAATTCTTCTGGAGGTTGCCATGCATAGATCTACTGCAGATATACAATATAAGCTATCTTGTATGAATATATTAGTGATTCTTGCTCGCTTCAGTCCATTGCTACTCAGTGGAACAGAAGAGGAACTGGTAAATTTTCTCAAAGATGACGATGAAGCTATAAAAGAAGGAGTTTTGAATGTTTTAGCAAAGGCTGGTGGCACCATCCGGGAAAATCTTGCAGCATTATCAAGTTCGATAGACCTTATATTGGAGAGGTTGTGTTTGGAGGGCAGTAGAAGACAGGCAAAATATGCTGTACATGCCTTGGCGGCAATAACAAAGGATGATGGGCTTAAATCACTTTCTGTTTTGTACAAGAGGCTTGTGGATATGCTGGAGGAGAAGACACATTTGCCTGCTGTACTACAATCGCTGGGGTGTATAGCTGAGACTGCAATGCCAGTCTTTGAAACTAGAGAAAGTGAAATTGAAAAGTTTATAACAGAGAAGATCCTAAAAAGCAATGATAAACCAGGTGATAATAAGAAGGCATCTTGGGATGACAAAAGTGAGCTTTGTGCCTTAAAGATATACGGAATCAAGACATTAGTGAAGAGCTACTTGCCTGTAAAAGATGCTCAGGTTCGCCCCGGCATTGATGGTCTGTTGGAAATCTTAAGAAACACACTTTCTTGTGGAGAAATATCCAAAGACATAGAATCAAGTTCAATTGACAAGGCACATTTAAGGCTCGCTTCTGCAAAAGCCGTTCTCCGTCTTTCAAAGCACTGGAATCATAAGATACCTGTTGATGTCTTCCACTTAACCTTGAAGGTTTCAGAGATTAGCTTCCCTCAAGCAAGGAGACTATTCCTTAACAAAGTTCATCAATACATAAAGGACCGCCTTTTGGATGCAAAATACACTTGTGCTTTCTTTTTCAACATGTTTGGATTGAAGTCAGCAGAATTTCAAGAGGAGAAACAGAATTTAGCTGACATTATTCAAATGTATCACCAAACGAAGGCTCGACATCTCTCAATTCAGTCTGATGCTAATTCCTTGACTGCTTATCCGGAGTACATCCTCCCATACTTGGTTCATGTGCTTGCTCATCATTGTTGTCCTAATATTGATGACTCCAAGGATGATGTCAAAGCTTTTGAGCCAATATACAGGCAATTGCACTTATTTCTATCTATGCTGCTACATAAAGATGAAGATGTCAAGTCGGAATCAACTAGCAACATTGAGAAGGAGGACCTTTCTGCTATAGTTTCTATCTTTCAAAGTATCAAGTCCTCAGAAGATATATATGATGTAGTGAAGTCAAAGAATTCACATGCTATTTGTGACCTTGGGCTGTCAATTACCAAACGCTTAGCCCCAAAGGAAATTGATTTACAGGTGTTGACTACATCAGTTCCCTTGCCTTCTATGCTGTATAAACCGTATGAGAAGAAGGAAGGAGATGACTCTGTGGCTAGTGAAGCACAAACGTGGCTGGCTGATGACAGTGTGCTGGCTCACTTTGAATCTCTTAAATTAGATACTACTGAAACGGATATTTCCGTAATTGCTGAGGATGAAGTGCTGATTGATGGCGAAAAAGATGGAAAGGAAGTGCCTTTGGGAAAAATAATAAAGCATTTGAAGTCTCAGAAGAACAAGGCTAAAAAAGAGAACAAAAACAAGGTTTCATCTGCTAATCCAGAGAAGGCTGAGAATGATGTTGATATCTTAAATATGGTGCGGGAGATCAATTTGGATAACTTGGGAGAATCAAGTAAGTTTGAATCAAGTAATGGTCACGAAAATTTGCCAAGCAGGAAATCAAGAACAGATACAAAACACCAAAAGGCTAACAAAAGAAAAACTAGTGATGGAGCATCTGTTGCAGTACCTAAACGGAGGAGGTCATCCACTGCTCATGGTGCTTTCAAATCACCCAGAAGTACTTCAAAGTCTCCTTTAAGTGCCTCACTGGATGATTCACTCAATAGAAAATTGGGTGAAAGCACTGAGTCAGCTTTGTTGGTATCATGCATCCGGAAGAATGCTACCTCCTCATCAAAACGTAAAAGCAGAGGTTCAGATCCTGTTCTTCATGATGAGGAAAATGAAGTTGGAGCAGACAGTGACCATGATGAGCCTGATGTGCTTGAGGCTGGCAAAAATGACCCGAATAGCGGGTACCAGTCTCCCACAGGACCTATCAAGAAGCGGAAAAAAAAGAGCATGTCAGGATCCACAAAGTCCAAATTCAAGGAAGGTGGAAAAGATATCGAAGATTTGATCGGTTGCAGAATAAAAGTTTGGTGGCCTATGGATAAAGCATTTTATGAAGGGACGGTGAAGTCTTATGATACTCTAAAGAGAAAGCATGTGGTACTTTATGCGGATGGTGATGTAGAAGTGCTTCGGCTAGAAAATGAACGGTGGGAGCTCATTGACAATGGCCGCAAGCCCACAAAGAAGTCCAATTCTTCAAAGAAATCCCCTTCAAAAGAAGTGTCGCCTGGTCAAAAAAGTAAAAGTGCGGGTAGTTCTCGTAAGAGCAAGAAATTGACTAAAACAGTCAAGGGGAAAAGAACACCGAGTAAAATATTGGACGGGAAAAGGGGCAGATCGAAGAGAAAACAGTGGGGAAGTAGGGAAAGAGAGAGTTCTGATGTGTCGAATATCGAGCCTAATTTGGTCTCTAAAGTGGATGAAATGAACTCAGGTAGTTCAGGTGGTGCTGAAAGAAAGGATGCGAATGTGTCAGATGAGGTGGACTCTGATAAGGAAGTGAAGTCGGTTTCCAAAGGAAATCTGCTGGAAGGGGCAGATTGTCCAAATCCAAATATTGAGGACTCTGATGAAGAGATGCCTGATTCTGAAGGGAGGCCTGCTAAAGATATGGACAGCATCGGACAAGATGCTCAAAATAATGGTGATGAGAAGCTTCCTTCAGAAGAGACTGAAACAGAGGAAGTAAATCGAGCCTCCTCAAGAGAGGGTAATGAGGAAGACTCGTCAGATTCTGAAGGGAATCAAGAAAAAGATGATGTCCGTGGTGGCCGTACAAAACAGAAGAAACCCCATCTACCTGTAGAGCCCTCAAGTCCTTCTGTAGCTGGGGATCTTGAACTTTCTGACGATGAGCCTCTTGGTAATTTCACACTCCGTGTAGGGAAAAGAGCTTCAAAGAGCGTACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1673

Amino Acids

187.43

Weight (kDa)

6.2

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 25 - 1088 2.5e-265 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2315
AarI CACCTGC 1 cut(s) 1516
AasI GACNNNNNNGTC 2 cut(s) 80, 4171
Acc36I ACCTGC 1 cut(s) 1516
Acc65I GGTACC 1 cut(s) 4008
AccB1I GGYRCC 2 cut(s) 2033, 4008
AccB7I CCANNNNNTGG 1 cut(s) 362
AccI GTMKAC 1 cut(s) 1208
AccIII TCCGGA 2 cut(s) 2890, 3864
AcoI YGGCCR 2 cut(s) 4273, 4876
AcuI CTGAAG 5 cut(s) 1047, 1909, 4716, 4752, 4863
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 4985
AfiI CCNNNNNNNGG 9 cut(s) 362, 1066, 1218, 1615, 2083, 2725, 2839, 2911, 4004
AflII CTTAAG 1 cut(s) 2491
AflIII ACRYGT 4 cut(s) 236, 988, 1512, 2760
AhdI GACNNNNNGTC 1 cut(s) 721
AhlI ACTAGT 1 cut(s) 3692
AjnI CCWGG 4 cut(s) 381, 1297, 2347, 4333
AjuI GAANNNNNNNTTGG 4 cut(s) 3924, 3956, 4114, 4146
AleI CACNNNNGTG 1 cut(s) 3313
Alw21I GWGCWC 2 cut(s) 1259, 4263
AlwNI CAGNNNCTG 2 cut(s) 3914, 4841
Aor13HI TCCGGA 2 cut(s) 2890, 3864
AoxI GGCC 4 cut(s) 4136, 4273, 4703, 4876
ApeKI GCWGC 7 cut(s) 22, 25, 101, 160, 623, 2054, 3019
ArsI GACNNNNNNTTYG 4 cut(s) 389, 421, 4313, 4345
Asp700I GAANNNNTTC 6 cut(s) 912, 1356, 1629, 1839, 3772, 4233
Asp718I GGTACC 1 cut(s) 4008
AspLEI GCGC 2 cut(s) 277, 1176
AspS9I GGNCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
AsuC2I CCSGG 4 cut(s) 256, 891, 2042, 2466
AsuII TTCGAA 1 cut(s) 970
AsuNHI GCTAGC 1 cut(s) 1222
AvaII GGWCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
BaeGI GKGCMC 1 cut(s) 326
BamHI GGATCC 2 cut(s) 41, 4061
BanI GGYRCC 2 cut(s) 2033, 4008
BanII GRGCYC 3 cut(s) 896, 4263, 4917
BarI GAAGNNNNNNTAC 2 cut(s) 4218, 4250
BbsI GAAGAC 5 cut(s) 1417, 2119, 2225, 2623, 4833
Bbv12I GWGCWC 2 cut(s) 1259, 4263
BbvCI CCTCAGC 2 cut(s) 1620, 3396
BbvI GCAGC 7 cut(s) 34, 37, 88, 172, 610, 2066, 3006
BceAI ACGGC 2 cut(s) 2567, 4863
BciT130I CCWGG 4 cut(s) 383, 1299, 2349, 4335
BclI TGATCA 4 cut(s) 487, 730, 832, 1642
BcnI CCSGG 4 cut(s) 256, 891, 2042, 2466
BcuI ACTAGT 1 cut(s) 3692
BfmI CTRYAG 4 cut(s) 1869, 3087, 4908, 4929
BfoI RGCGCY 2 cut(s) 278, 1177
BfrI CTTAAG 1 cut(s) 2491
BfuAI ACCTGC 1 cut(s) 1516
BglI GCCNNNNNGGC 1 cut(s) 2144
BglII AGATCT 1 cut(s) 1862
BisI GCNGC 9 cut(s) 23, 26, 102, 161, 624, 2055, 2145, 3020, 4276
BlpI GCTNAGC 1 cut(s) 3196
BlsI GCNGC 9 cut(s) 24, 27, 103, 162, 625, 2056, 2146, 3021, 4277
BmcAI AGTACT 2 cut(s) 1783, 3772
Bme1390I CCNGG 8 cut(s) 256, 383, 891, 1299, 2042, 2349, 2466, 4335
Bme18I GGWCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
BmeRI GACNNNNNGTC 1 cut(s) 721
BmgT120I GGNCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
BmiI GGNNCC 5 cut(s) 43, 909, 2035, 4010, 4063
BmrFI CCNGG 8 cut(s) 256, 383, 891, 1299, 2042, 2349, 2466, 4335
BmtI GCTAGC 1 cut(s) 1226
BpiI GAAGAC 5 cut(s) 1417, 2119, 2225, 2623, 4833
BplI GAGNNNNNCTC 4 cut(s) 4577, 4609, 4814, 4846
BpmI CTGGAG 5 cut(s) 404, 837, 1493, 1865, 2231
Bpu10I CCTNAGC 3 cut(s) 1620, 2454, 3396
Bpu1102I GCTNAGC 1 cut(s) 3196
Bpu14I TTCGAA 1 cut(s) 970
BpuEI CTTGAG 7 cut(s) 14, 77, 1481, 2655, 3998, 4792, 4903
BpuMI CCSGG 4 cut(s) 256, 891, 2042, 2466
BsaBI GATNNNNATC 1 cut(s) 768
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 4519
BsaJI CCNNGG 8 cut(s) 147, 1212, 1298, 2139, 2464, 2958, 3172, 4870
BsaWI WCCGGW 2 cut(s) 2890, 3864
BsaXI ACNNNNNCTCC 4 cut(s) 2078, 2108, 3693, 3723
Bsc4I CCNNNNNNNGG 9 cut(s) 362, 1066, 1218, 1615, 2083, 2725, 2839, 2911, 4004
Bse118I RCCGGY 1 cut(s) 1059
Bse1I ACTGG 8 cut(s) 820, 1278, 1476, 1965, 2276, 2609, 3804, 4012
Bse3DI GCAATG 4 cut(s) 519, 1931, 2277, 2344
Bse8I GATNNNNATC 1 cut(s) 768
BseAI TCCGGA 2 cut(s) 2890, 3864
BseBI CCWGG 4 cut(s) 383, 1299, 2349, 4335
BseDI CCNNGG 8 cut(s) 147, 1212, 1298, 2139, 2464, 2958, 3172, 4870
BseJI GATNNNNATC 1 cut(s) 768
BseLI CCNNNNNNNGG 9 cut(s) 362, 1066, 1218, 1615, 2083, 2725, 2839, 2911, 4004
BseMI GCAATG 4 cut(s) 519, 1931, 2277, 2344
BseNI ACTGG 8 cut(s) 820, 1278, 1476, 1965, 2276, 2609, 3804, 4012
BseRI GAGGAG 7 cut(s) 80, 123, 2228, 2807, 3742, 3871, 4795
BseSI GKGCMC 1 cut(s) 326
BseXI GCAGC 7 cut(s) 34, 37, 88, 172, 610, 2066, 3006
BseYI CCCAGC 2 cut(s) 2248, 4934
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 4 cut(s) 4138, 4275, 4705, 4878
BshNI GGYRCC 2 cut(s) 2033, 4008
BsiHKAI GWGCWC 2 cut(s) 1259, 4263
BsiSI CCGG 7 cut(s) 256, 890, 1060, 2041, 2466, 2891, 3865
BsiWI CGTACG 1 cut(s) 5011
BslFI GGGAC 1 cut(s) 4177
BslI CCNNNNNNNGG 9 cut(s) 362, 1066, 1218, 1615, 2083, 2725, 2839, 2911, 4004
BsmBI CGTCTC 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 4177
BsmI GAATGC 1 cut(s) 3877
BsnI GGCC 4 cut(s) 4138, 4275, 4705, 4878
Bso31I GGTCTC 1 cut(s) 4519
Bsp119I TTCGAA 1 cut(s) 970
Bsp1286I GDGCHC 5 cut(s) 326, 896, 1259, 4263, 4917
Bsp13I TCCGGA 2 cut(s) 2890, 3864
Bsp1407I TGTACA 2 cut(s) 2131, 2187
Bsp1720I GCTNAGC 1 cut(s) 3196
Bsp19I CCATGG 1 cut(s) 1212
BspANI GGCC 4 cut(s) 4138, 4275, 4705, 4878
BspEI TCCGGA 2 cut(s) 2890, 3864
BspHI TCATGA 4 cut(s) 525, 748, 1126, 3922
BspLI GGNNCC 5 cut(s) 43, 909, 2035, 4010, 4063
BspMAI CTGCAG 1 cut(s) 1873
BspMI ACCTGC 1 cut(s) 1516
BspOI GCTAGC 1 cut(s) 1226
BspQI GCTCTTC 1 cut(s) 2423
BspT104I TTCGAA 1 cut(s) 970
BspT107I GGYRCC 2 cut(s) 2033, 4008
BspTI CTTAAG 1 cut(s) 2491
BspTNI GGTCTC 1 cut(s) 4519
BsrDI GCAATG 4 cut(s) 519, 1931, 2277, 2344
BsrFI RCCGGY 1 cut(s) 1059
BsrGI TGTACA 2 cut(s) 2131, 2187
BsrI ACTGG 8 cut(s) 820, 1278, 1476, 1965, 2276, 2609, 3804, 4012
BssAI RCCGGY 1 cut(s) 1059
BssECI CCNNGG 8 cut(s) 147, 1212, 1298, 2139, 2464, 2958, 3172, 4870
BssNAI GTATAC 1 cut(s) 1209
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 5 cut(s) 147, 1212, 2139, 2958, 3172
Bst1107I GTATAC 1 cut(s) 1209
Bst2UI CCWGG 4 cut(s) 383, 1299, 2349, 4335
Bst6I CTCTTC 9 cut(s) 537, 596, 935, 1071, 1947, 2423, 4442, 4674, 4767
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 2491
BstAPI GCANNNNNTGC 2 cut(s) 2126, 3710
BstAUI TGTACA 2 cut(s) 2131, 2187
BstBI TTCGAA 1 cut(s) 970
BstDSI CCRYGG 2 cut(s) 1212, 4870
BstH2I RGCGCY 2 cut(s) 278, 1177
BstHHI GCGC 2 cut(s) 277, 1176
BstNI CCWGG 4 cut(s) 383, 1299, 2349, 4335
BstSCI CCNGG 8 cut(s) 254, 381, 889, 1297, 2040, 2347, 2464, 4333
BstSFI CTRYAG 4 cut(s) 1869, 3087, 4908, 4929
BstSLI GKGCMC 1 cut(s) 326
BstV1I GCAGC 7 cut(s) 34, 37, 88, 172, 610, 2066, 3006
BstV2I GAAGAC 5 cut(s) 1417, 2119, 2225, 2623, 4833
BstX2I RGATCY 7 cut(s) 41, 1554, 1862, 2325, 3910, 4061, 4939
BstYI RGATCY 7 cut(s) 41, 1554, 1862, 2325, 3910, 4061, 4939
BstZ17I GTATAC 1 cut(s) 1209
BsuRI GGCC 4 cut(s) 4138, 4275, 4705, 4878
BtgI CCRYGG 2 cut(s) 1212, 4870
BtsI GCAGTG 2 cut(s) 884, 3738
BveI ACCTGC 1 cut(s) 1516
CaiI CAGNNNCTG 2 cut(s) 3914, 4841
CciI TCATGA 4 cut(s) 525, 748, 1126, 3922
CfoI GCGC 2 cut(s) 277, 1176
Cfr10I RCCGGY 1 cut(s) 1059
Cfr13I GGNCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
CseI GACGC 1 cut(s) 83
CsiI ACCWGGT 1 cut(s) 2347
CspCI CAANNNNNGTGG 2 cut(s) 4069, 4104
DraI TTTAAA 1 cut(s) 852
DraIII CACNNNGTG 1 cut(s) 4985
DrdI GACNNNNNNGTC 2 cut(s) 80, 4171
DriI GACNNNNNGTC 1 cut(s) 721
DseDI GACNNNNNNGTC 2 cut(s) 80, 4171
EaeI YGGCCR 2 cut(s) 4273, 4876
Eam1104I CTCTTC 9 cut(s) 537, 596, 935, 1071, 1947, 2423, 4442, 4674, 4767
Eam1105I GACNNNNNGTC 1 cut(s) 721
EarI CTCTTC 9 cut(s) 537, 596, 935, 1071, 1947, 2423, 4442, 4674, 4767
Ecl136II GAGCTC 1 cut(s) 4261
Eco130I CCWWGG 5 cut(s) 147, 1212, 2139, 2958, 3172
Eco147I AGGCCT 1 cut(s) 4705
Eco24I GRGCYC 3 cut(s) 896, 4263, 4917
Eco31I GGTCTC 1 cut(s) 4519
Eco32I GATATC 3 cut(s) 613, 3553, 4099
Eco47I GGWCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
Eco53kI GAGCTC 1 cut(s) 4261
Eco57I CTGAAG 5 cut(s) 1047, 1909, 4716, 4752, 4863
EcoICRI GAGCTC 1 cut(s) 4261
EcoO109I RGGNCCY 2 cut(s) 3076, 4025
EcoRI GAATTC 2 cut(s) 1385, 3151
EcoRII CCWGG 4 cut(s) 381, 1297, 2347, 4333
EcoRV GATATC 3 cut(s) 613, 3553, 4099
EcoT14I CCWWGG 5 cut(s) 147, 1212, 2139, 2958, 3172
EcoT22I ATGCAT 2 cut(s) 1860, 3863
EcoT38I GRGCYC 3 cut(s) 896, 4263, 4917
ErhI CCWWGG 5 cut(s) 147, 1212, 2139, 2958, 3172
Esp3I CGTCTC 1 cut(s) 63
FalI AAGNNNNNCTT 6 cut(s) 552, 584, 2420, 2452, 2554, 2586
FaqI GGGAC 1 cut(s) 4177
FauI CCCGC 5 cut(s) 145, 655, 3561, 3998, 4348
FbaI TGATCA 4 cut(s) 487, 730, 832, 1642
FblI GTMKAC 1 cut(s) 1208
Fnu4HI GCNGC 9 cut(s) 23, 26, 102, 161, 624, 2055, 2145, 3020, 4276
FriOI GRGCYC 3 cut(s) 896, 4263, 4917
Fsp4HI GCNGC 9 cut(s) 23, 26, 102, 161, 624, 2055, 2145, 3020, 4276
GlaI GCGC 2 cut(s) 276, 1175
GluI GCNGC 9 cut(s) 23, 26, 102, 161, 624, 2055, 2145, 3020, 4276
GsaI CCCAGC 2 cut(s) 2252, 4938
GsuI CTGGAG 5 cut(s) 404, 837, 1493, 1865, 2231
HaeII RGCGCY 2 cut(s) 278, 1177
HaeIII GGCC 4 cut(s) 4138, 4275, 4705, 4878
HapII CCGG 7 cut(s) 256, 890, 1060, 2041, 2466, 2891, 3865
HgaI GACGC 1 cut(s) 83
HhaI GCGC 2 cut(s) 277, 1176
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 275, 1174
HinP1I GCGC 2 cut(s) 275, 1174
HincII GTYRAC 4 cut(s) 726, 862, 948, 3228
HindII GTYRAC 4 cut(s) 726, 862, 948, 3228
HindIII AAGCTT 4 cut(s) 221, 668, 2973, 4760
HpaI GTTAAC 1 cut(s) 862
HpaII CCGG 7 cut(s) 256, 890, 1060, 2041, 2466, 2891, 3865
Hpy166II GTNNAC 6 cut(s) 726, 862, 948, 1209, 3228, 4591
Hpy8I GTNNAC 6 cut(s) 726, 862, 948, 1209, 3228, 4591
Hpy99I CGWCG 1 cut(s) 10
HpyCH4IV ACGT 3 cut(s) 464, 3314, 3892
HpySE526I ACGT 3 cut(s) 464, 3314, 3892
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 275, 1174
Kpn2I TCCGGA 2 cut(s) 2890, 3864
KpnI GGTACC 1 cut(s) 4012
Ksp22I TGATCA 4 cut(s) 487, 730, 832, 1642
KspAI GTTAAC 1 cut(s) 862
LguI GCTCTTC 1 cut(s) 2423
Lsp1109I GCAGC 7 cut(s) 34, 37, 88, 172, 610, 2066, 3006
MabI ACCWGGT 1 cut(s) 2347
MaeII ACGT 3 cut(s) 464, 3314, 3892
MaeIII GTNAC 7 cut(s) 445, 1055, 1488, 1515, 3167, 3623, 3953
MfeI CAATTG 3 cut(s) 1646, 2544, 2996
MflI RGATCY 7 cut(s) 41, 1554, 1862, 2325, 3910, 4061, 4939
MhlI GDGCHC 5 cut(s) 326, 896, 1259, 4263, 4917
MlyI GAGTC 9 cut(s) 47, 161, 382, 2948, 3284, 3848, 4586, 4664, 4823
MmeI TCCRAC 7 cut(s) 102, 402, 578, 1047, 2463, 3027, 3922
Mph1103I ATGCAT 2 cut(s) 1860, 3863
MroI TCCGGA 2 cut(s) 2890, 3864
MroXI GAANNNNTTC 6 cut(s) 912, 1356, 1629, 1839, 3772, 4233
MslI CAYNNNNRTG 3 cut(s) 1536, 3030, 3313
MspA1I CMGCKG 1 cut(s) 838
MspCI CTTAAG 1 cut(s) 2491
MspI CCGG 7 cut(s) 256, 890, 1060, 2041, 2466, 2891, 3865
MspR9I CCNGG 8 cut(s) 256, 383, 891, 1299, 2042, 2349, 2466, 4335
MunI CAATTG 3 cut(s) 1646, 2544, 2996
Mva1269I GAATGC 1 cut(s) 3877
MvaI CCWGG 4 cut(s) 383, 1299, 2349, 4335
NciI CCSGG 4 cut(s) 256, 891, 2042, 2466
NcoI CCATGG 1 cut(s) 1212
NheI GCTAGC 1 cut(s) 1222
NlaIV GGNNCC 5 cut(s) 43, 909, 2035, 4010, 4063
NmeAIII GCCGAG 1 cut(s) 1195
NmuCI GTSAC 6 cut(s) 445, 1055, 1515, 3167, 3623, 3953
NsiI ATGCAT 2 cut(s) 1860, 3863
NspV TTCGAA 1 cut(s) 970
OliI CACNNNNGTG 1 cut(s) 3313
PagI TCATGA 4 cut(s) 525, 748, 1126, 3922
PaqCI CACCTGC 1 cut(s) 1516
PceI AGGCCT 1 cut(s) 4705
PciI ACATGT 4 cut(s) 236, 988, 1512, 2760
PciSI GCTCTTC 1 cut(s) 2423
PctI GAATGC 1 cut(s) 3877
PdmI GAANNNNTTC 6 cut(s) 912, 1356, 1629, 1839, 3772, 4233
Pfl23II CGTACG 1 cut(s) 5011
PflMI CCANNNNNTGG 1 cut(s) 362
PfoI TCCNGGA 2 cut(s) 381, 2040
PkrI GCNGC 9 cut(s) 24, 27, 103, 162, 625, 2056, 2146, 3021, 4277
PleI GAGTC 9 cut(s) 47, 160, 382, 2948, 3284, 3847, 4586, 4664, 4823
PpsI GAGTC 9 cut(s) 47, 160, 382, 2948, 3284, 3847, 4586, 4664, 4823
PpuMI RGGWCCY 2 cut(s) 3076, 4025
PscI ACATGT 4 cut(s) 236, 988, 1512, 2760
PsiI TTATAA 1 cut(s) 2315
Psp124BI GAGCTC 1 cut(s) 4263
Psp5II RGGWCCY 2 cut(s) 3076, 4025
Psp6I CCWGG 4 cut(s) 381, 1297, 2347, 4333
PspFI CCCAGC 2 cut(s) 2248, 4934
PspGI CCWGG 4 cut(s) 381, 1297, 2347, 4333
PspLI CGTACG 1 cut(s) 5011
PspN4I GGNNCC 5 cut(s) 43, 909, 2035, 4010, 4063
PspPI GGNCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
PspPPI RGGWCCY 2 cut(s) 3076, 4025
PstI CTGCAG 1 cut(s) 1873
PstNI CAGNNNCTG 2 cut(s) 3914, 4841
PsuI RGATCY 7 cut(s) 41, 1554, 1862, 2325, 3910, 4061, 4939
PvuII CAGCTG 1 cut(s) 838
RseI CAYNNNNRTG 3 cut(s) 1536, 3030, 3313
SacI GAGCTC 1 cut(s) 4263
SapI GCTCTTC 1 cut(s) 2423
SatI GCNGC 9 cut(s) 23, 26, 102, 161, 624, 2055, 2145, 3020, 4276
Sau96I GGNCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
ScaI AGTACT 2 cut(s) 1783, 3772
SchI GAGTC 9 cut(s) 47, 161, 382, 2948, 3284, 3848, 4586, 4664, 4823
ScrFI CCNGG 8 cut(s) 256, 383, 891, 1299, 2042, 2349, 2466, 4335
SduI GDGCHC 5 cut(s) 326, 896, 1259, 4263, 4917
SexAI ACCWGGT 1 cut(s) 2347
SfcI CTRYAG 4 cut(s) 1869, 3087, 4908, 4929
SfuI TTCGAA 1 cut(s) 970
SinI GGWCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
SmiMI CAYNNNNRTG 3 cut(s) 1536, 3030, 3313
SmlI CTYRAG 8 cut(s) 29, 92, 1460, 2491, 2670, 3977, 4807, 4918
SmoI CTYRAG 8 cut(s) 29, 92, 1460, 2491, 2670, 3977, 4807, 4918
SpeI ACTAGT 1 cut(s) 3692
SseBI AGGCCT 1 cut(s) 4705
SspI AATATT 3 cut(s) 2947, 4422, 4663
SstI GAGCTC 1 cut(s) 4263
StuI AGGCCT 1 cut(s) 4705
StyD4I CCNGG 8 cut(s) 254, 381, 889, 1297, 2040, 2347, 2464, 4333
StyI CCWWGG 5 cut(s) 147, 1212, 2139, 2958, 3172
TaiI ACGT 3 cut(s) 467, 3317, 3895
TaqII GACCGA 1 cut(s) 967
TatI WGTACW 9 cut(s) 502, 1192, 1509, 1781, 2131, 2187, 2236, 2895, 3770
TauI GCSGC 2 cut(s) 2147, 4278
TseFI GTSAC 6 cut(s) 445, 1055, 1515, 3167, 3623, 3953
TseI GCWGC 7 cut(s) 22, 25, 101, 160, 623, 2054, 3019
Tsp45I GTSAC 6 cut(s) 445, 1055, 1515, 3167, 3623, 3953
TspGWI ACGGA 7 cut(s) 2424, 2579, 3376, 3392, 3739, 4859, 4973
Van91I CCANNNNNTGG 1 cut(s) 362
Vha464I CTTAAG 1 cut(s) 2491
VpaK11BI GGWCC 6 cut(s) 353, 424, 979, 2716, 3076, 4025
XmiI GTMKAC 1 cut(s) 1208
XmnI GAANNNNTTC 6 cut(s) 912, 1356, 1629, 1839, 3772, 4233
ZrmI AGTACT 2 cut(s) 1783, 3772
Zsp2I ATGCAT 2 cut(s) 1860, 3863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.