Rw5G000400

Sister chromatid cohesion protein PDS5 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0009376
Physical Location & Seq
Reverse (-)
325364 .. 337677
12314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000400.1

Sequence Viewer

Length: 5016 bp
ATGGCCCAGAAGGTTGAGCAGCAGCTCAAGGAGGTGGGATCCAAGCTCGAGACCCCTCATTCGTCCAAGGACGCTCTCGTCAAGCTCCTCAAGCAAGCTCAGTTATGTCTTTCTGAGTTGGATCAGTCACCTCCAGCATCTACCTTGGAGTCAATGCAGCCTTTTCTTAATGCAATAGTCAAGCCAGAACTGTTGAAGCATCAAGATAGAGATGTCAAGCTTCTAGTAGCAACATGTATTTGTGAGATAACCCGGATAACTGCACCAGAAGCTCCTTATAGTGATGATGTGCTAAAGGATGTTTTTCACCTGATTGTGGGCACTTTTAGTGGATTGAAAGATACAAGTGGTCCATCATTTGGTAGGAGAGTTGTCATACTGGAGACTCTTGCAAAGTATAGGTCATGTGTTGTAATGCTGGACCTCGAATGTGATGATCTGGTGACTGAAATGTTCAGTACATTTTTTGCTGTTGCCAGAGATGATCACCAAGAAACTGTACTGTCATCAATGCAAACAATCATGATTGTTCTTATAGAAGAGAGCGAGGATTTGCAAGAGGACCTTTTATTTGTCATCTTGTCTGTATTAGGTCGTAAGAGAAGTGATATCAATGTGGCTGCTAGGAGACTTGCTATGAATGTTATAGAACAGTGCGCAGGAAAGCTTGAATCCGGTATCAAACAGTTCCTTATATCATCAATGTCAGGCGACAACAAGTCAACCAGTCATCAAATTGATTACCATGAAGTCATCTATGATGTATATCGTTGTGCCCCCCAAATTGTGTCAGCAGTTGTCCCTTACCTAACTGGAGAGCTACTGACTGATCAGCTCGACACTCGTTTAAAAGCAGTTAACTTAGTTGGAGACTTATTTTCACTGCCGGGCTCTACCATTTCAGAACCATTTCAGCCAATTTTTTCAGAATTTTTGAAGAGGTTGACTGATAGAGTTGTTGAGGTTCGAATGTCAGTCCTTGAACATGTCAAGAGCTGTATGATGTCAAATCCTTTCAGAGCTGAAGCTCCCGAAATAATCTCTGCCCTCTGTGACCGGCTGTTGGACTTCGAAGAGAAAGTTCGGAAGCAAGTTGTTGCTGTAATTTATGATGTGGCATGTCATGACCTAAATTCCATTCCGCTAGAAACTGTACAACTTGTTGCCGAGCGCCTTCGAGACAAATCTGTACTTGTTAAAAAGTATACCATGGAGAGGCTGGCTGAGATATACAGGGTTTATTGTGCAAAGTGCTCTGATGGCTCGACCATTTCCAGTGAATTTGAGTGGATTCCTGGGAAGATTCTGAGATGTTTTTATGACAAAGATTTCAGATCTGATACAATTGAAAATGTTCTTTGTGAATCTCTATTCCCAACTGATTTCTCTACCAAAGATAAAGTTAAACATTGGGTAAGGGTCTTCTCAGTATTTGATAAAGTTGAGGTGAAGGCACTTGAGAAGATACTGGAGCAGAAGCAAAGGTTACTACAAGAGATGCAGAAGTATATGTCACTTAGGCAGGTGCATCAGGATGGTGATGCTCCTGAGATCCAAAAAAAGATTCAGTTTTGTTTTCGTATAATGGCACGCTCGTTTGCTGACCCTGCAAAGGCTGAGGAAAATTTTCAGTTTCTTGATCAATTGAAGGATGCTAACATCTGGAAGATTCTAACGAACCTTGTTGATCCAAACACTAGCTTCCATCAAGCCCGAACTTTACGGGATGAATTGCTTAAAATTTTGGGCGAAAAACACCGACTATATGACTTTCTTAGTACTCTCTCCGTGAAATGTTCTTATCTACTTTTCAACAAGGAGCATGTGAAGGAAATTCTTCTGGAGGTTGCCATGCACAGATCTACTGCAGATATACAATATAAGCTATCTTGTATGAATATTTTGGTGATTCTTGCTCGCTTCAGTCCATTGCTACTTAGTGGAACAGAAGAGGAACTGGTAAATTTTCTCAAAGATGATGATGAAGCTATAAAAGAAGGAGTTTTGAATGTTTTAGCAAAGGCTGGTGGTACCATCCGGGAAAATCTTGCAGTGTTATCAAGTTCGATAGACCTTATATTGGAGAGGTTGTGCTTGGAGGGCAGTAGAAGACAGGCAAAATATGCTGTACATGCCTTGGCGGCTATAACAAAGGATGATGGGCTTAAATCACTTTCTGTTTTGTACAAGAGGCTTGTGGATATGCTCGAGGAGAAGACACATTTGCCTGCTGTACTACAATCTTTGGGGTGTATAGCTGAGACTGCAATGCCAGTCTTCGAAACTAGAGAAAGTGAAATTGAAAAGTTTATAATAGAGAAGATCCTAAAACGCAATGATAAACCAGGTGATAATAAGAAGGCAACTTGGGATGACAAAAGTGAGCTTTGTTCCTTAAAGATATATGGAATTAAGACCTTGGTGAAAAGCTACTTGCCTGTCAAAGATGCTCAGGTTCGCCCCGGCATTGATGGTCTACTGGAAATATTAAGAAATACACTTTCTTGTGGAGAAATATCCAAAGACATAGAATCAAGTTCAATTGACAAGGCCCATTTAAGGCTTGCTTCTGCAAAGGCCGTTCTCCGTCTATCAAAGCACTGGAATCATAAGATACCTGTTGATGTCGTCCACTTAACCTTGAAGGTTTCAGAGATTAGCTTCCCTCAAGCAAGGAAACTATTCCTTACCAAAGTTCATCAATACATAAAGGACCGGCTTTTGGATGCAAAATATGCTTGTGCATTCTTTTTCAACATATTTGGATCAAAGTTGGCAGAATTTCAAGAGGAGAAACAAAATCTAGCTGACATTATTCAAATGTATCACCAAACAAAGGCTCGACATCTCTCTATTCAATCTGATGCTAATTCCTTGACTGCATATCCGGAGTACATCCTCCCTTACTTGGTTCATGCGCTTGCCCATCATTGTTGTCCTAATATTGATGAATCCAAGGATGATGTCAAAGCTTTTGAACTGATATACAGGCAATTGCACTTAATTCTGTCTATGTTGCTACATAGAGATGAAGATGTCAAGTCGGAATCAGGTAGCAACATTGAAAAGGAGGACCTTTCTGCTATAGTCTCTATCTTTCACAGTATCAAGACCTCTGAAGATATATATGATGCAGTGAAGTCAAAGAATTCACATGCTATTTGTGACCTTGGGTTGTCAATTACCAAGCGCTTAGCCCCAAAGGAAACTGATTTACAAGTGTTGACTGTGTCAGTTCCTTTGCCTTCTATGCTATATAAACCGTATGAGAAAAAGGAAGGAGATGATTCAGTGGCTATTGAAGCACAAACGTGGTTGGCTGATGACAGTGTGCTGACTCACTTTGAATCACTTAAGTTAGACACTATTGAAACGGGAATTTCCATAATTGCTGAGGATGAAGTTCTGATAGATGGTGAAAAAGATGGAAATGAAGTGCCTTTGGGAAAAATTATAAAGCACTTGAAGTCTCAGAAGATCAAGGCTAAAAAGGTGAAAAAGAACAAGGTTTCATCTGCTAATCCAGAGAAGGCTGAGAATGATGTTGATATCTTGAATATGGTCCGGGAGATAAATTTGGATAACTTGGGAGAATCAAGTAAGTTTGAATCAAGTAATGGTCATGAAAATCTGCCCGGCAAGAAATCAAGAATAGATACAACGCACCAAAAGGGTAACAAAAGAAAAGCTAGTGATGGAGCATCTGTTCCAGTACCTAAACGGAGGAGGTCATCCTCCACTCATAGTGCTATCAAATCTCCCAGAAGTACATCAAAGTCTCCTTTAAGCGCTTCACTTGATGATTCACACAATAGAAAATTGGGTGAAAGCATGGAGTCAGCTTTGTTGGTATCGTGCATCCGGAAGAATGCTACCTCCTCATCAAAACGTAAAGGCAGAGCTTCAGATCCTGGTCTTCATGATGAGGAAAATGAAGTAGGAGAAGACAGTGACCGTGACGAGCCCAATGTGCTTGAGACTGGCAAGAAGGACCCAAATAATGAGTACCAGTCTCCCACAAGACCCATCAAGAAGCCGAAAAGAAAAAGCATGTCAGGATCTGCAAAGTCCAAATTCAAGGAAGGTGGAAAAGATATCGAAGACTTGATTGGCTGCAGAATAAAAGTGTGGTGGCCTATGGATAGGAAATTTTATGAAGGGACGGTGAAGTCTTATGATACACTAAAGAGAAAGCATGTGGTACTTTATGCGGATGGTGATGTAGAAGTGCTTCGGCTAGAAAAGGAACGGTGGGAGCTCGTTGACAATGGTCGCAAGCCCACAAAGAAGTCAAATTCTTCAAAGAAATCCCCTTCAAAAGAAGTGACACCTGGTCAAAAAAGTAAAAGTATGGGTAGTTCTAGTAAGAGCAAGAAATCCACTAAAACAGTTAAGAAGAAAAGAACACCGAGTAAAAACTTGGACGGGAAAAAGGGTGGATCAAAGAGAAAACAGTGGGGAAGTAGGGAAAGAGAGAGTTCTGATGTGTCGAATATTGAGCCTAATTTGGTCTCTAAAGTGGATGAAATGAACTCAGGTAGTTCAGGAGGTGCTGAAAGAAAGGATGTGAATGTGTCAGAGGAGGTGGACTCTGACAAGGAAGTGAAGTCAGTTTCCAAAGGAAATCTGCTGGAAGAGGCAGAAAGTCCAAATCCAAATATTGAGGACTCTGATGAAGAGATGCCTGATTCTGAAGTGAGGCCTGCTGGAGATATGGACAGCATCGGACAAGATACTGAAAATACAGCTGACAAGAAGCTTCCTTCAGAAGAGAAACAAACAGATGAATTAAATAGATCATCCTCGAGAGAGGAGAATGAGGAAGAGTCGTCAGATTCTGAAGGGAGTAAAGAAAAAAATGATGTCCATGGTGACCGTACAAAACTGAAGAAACCCCATGTACCTGTGGAACCCTCAAGCCCTTCTGATGCTGGGGATCTTGAACTTTCCGATGATGAACCCCTTAGCAATTTTACACTCCGTGTAGGGAAAAGAGCTTCAAAGAGAGTACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1671

Amino Acids

187.94

Weight (kDa)

6.35

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 24 - 1087 3.4e-267 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2312, 3455
AarI CACCTGC 1 cut(s) 1513
AasI GACNNNNNNGTC 2 cut(s) 77, 4168
Acc16I TGCGCA 1 cut(s) 658
Acc36I ACCTGC 1 cut(s) 1513
Acc65I GGTACC 1 cut(s) 2030
AccB1I GGYRCC 1 cut(s) 2030
AccB7I CCANNNNNTGG 1 cut(s) 359
AccI GTMKAC 2 cut(s) 1205, 2476
AccIII TCCGGA 2 cut(s) 2887, 3861
AciI CCGC 3 cut(s) 1142, 2141, 4211
AcuI CTGAAG 8 cut(s) 1044, 1906, 3138, 3888, 4713, 4749, 4860, 4907
AdeI CACNNNGTG 1 cut(s) 4982
AfeI AGCGCT 2 cut(s) 3191, 3790
AflII CTTAAG 1 cut(s) 3353
AflIII ACRYGT 2 cut(s) 233, 985
AhdI GACNNNNNGTC 2 cut(s) 718, 4332
AjnI CCWGG 4 cut(s) 1294, 2344, 3910, 4330
AjuI GAANNNNNNNTTGG 4 cut(s) 1548, 1580, 4092, 4124
AleI CACNNNNGTG 1 cut(s) 3310
AloI GAACNNNNNNTCC 2 cut(s) 3690, 3722
Alw21I GWGCWC 2 cut(s) 1256, 4260
AlwNI CAGNNNCTG 3 cut(s) 3911, 4061, 4838
Ama87I CYCGRG 3 cut(s) 47, 2207, 4804
Aor13HI TCCGGA 2 cut(s) 2887, 3861
Aor51HI AGCGCT 2 cut(s) 3191, 3790
AoxI GGCC 5 cut(s) 3, 2550, 2577, 4133, 4700
ApeKI GCWGC 5 cut(s) 19, 22, 157, 620, 4113
ArsI GACNNNNNNTTYG 6 cut(s) 43, 75, 386, 418, 554, 586
Asp700I GAANNNNTTC 6 cut(s) 909, 1353, 1626, 1836, 2681, 4230
Asp718I GGTACC 1 cut(s) 2030
AspLEI GCGC 5 cut(s) 659, 1173, 2920, 3192, 3791
AspS9I GGNCC 9 cut(s) 4, 350, 421, 562, 2551, 2713, 3073, 3562, 3991
AsuC2I CCSGG 6 cut(s) 253, 888, 2039, 2463, 3566, 3636
AsuII TTCGAA 3 cut(s) 967, 1071, 2280
AvaI CYCGRG 3 cut(s) 47, 2207, 4804
AvaII GGWCC 7 cut(s) 350, 421, 562, 2713, 3073, 3562, 3991
BaeGI GKGCMC 2 cut(s) 323, 778
BamHI GGATCC 1 cut(s) 38
BanI GGYRCC 1 cut(s) 2030
BanII GRGCYC 3 cut(s) 893, 3966, 4260
BarI GAAGNNNNNNTAC 2 cut(s) 4215, 4247
BbsI GAAGAC 7 cut(s) 1414, 2116, 2222, 2269, 3908, 3951, 4107
Bbv12I GWGCWC 2 cut(s) 1256, 4260
BbvCI CCTCAGC 2 cut(s) 1617, 3393
BbvI GCAGC 5 cut(s) 31, 34, 169, 607, 4100
BceAI ACGGC 1 cut(s) 2564
BciT130I CCWGG 4 cut(s) 1296, 2346, 3912, 4332
BclI TGATCA 3 cut(s) 484, 829, 1639
BcnI CCSGG 6 cut(s) 253, 888, 2039, 2463, 3566, 3636
BfaI CTAG 9 cut(s) 224, 624, 1145, 1698, 2286, 2804, 3690, 4238, 4362
BfmI CTRYAG 3 cut(s) 1866, 3084, 4114
BfoI RGCGCY 3 cut(s) 1174, 3193, 3792
BfrI CTTAAG 1 cut(s) 3353
BfuAI ACCTGC 1 cut(s) 1513
BglI GCCNNNNNGGC 1 cut(s) 2141
BglII AGATCT 2 cut(s) 1334, 1859
BisI GCNGC 6 cut(s) 20, 23, 158, 621, 2142, 4114
BlpI GCTNAGC 1 cut(s) 3193
BlsI GCNGC 6 cut(s) 21, 24, 159, 622, 2143, 4115
BmcAI AGTACT 1 cut(s) 1780
Bme18I GGWCC 7 cut(s) 350, 421, 562, 2713, 3073, 3562, 3991
BmeRI GACNNNNNGTC 2 cut(s) 718, 4332
BmeT110I CYCGRG 3 cut(s) 47, 2207, 4804
BmgT120I GGNCC 9 cut(s) 4, 350, 421, 562, 2551, 2713, 3073, 3562, 3991
BmiI GGNNCC 4 cut(s) 40, 2032, 3993, 4911
BoxI GACNNNNGTC 1 cut(s) 4269
BpiI GAAGAC 7 cut(s) 1414, 2116, 2222, 2269, 3908, 3951, 4107
BplI GAGNNNNNCTC 2 cut(s) 4574, 4606
BpmI CTGGAG 6 cut(s) 117, 401, 834, 1490, 1862, 4728
Bpu10I CCTNAGC 4 cut(s) 1617, 2451, 3393, 4964
Bpu1102I GCTNAGC 1 cut(s) 3193
Bpu14I TTCGAA 3 cut(s) 967, 1071, 2280
BpuEI CTTGAG 6 cut(s) 11, 74, 1478, 2652, 3995, 4900
BpuMI CCSGG 6 cut(s) 253, 888, 2039, 2463, 3566, 3636
BsaBI GATNNNNATC 1 cut(s) 765
BsaI GGTCTC 2 cut(s) 44, 4516
BsaWI WCCGGW 3 cut(s) 674, 2887, 3861
BsaXI ACNNNNNCTCC 4 cut(s) 2075, 2105, 3690, 3720
Bse118I RCCGGY 2 cut(s) 1056, 2715
Bse3DI GCAATG 3 cut(s) 1928, 2274, 2341
Bse8I GATNNNNATC 1 cut(s) 765
BseAI TCCGGA 2 cut(s) 2887, 3861
BseBI CCWGG 4 cut(s) 1296, 2346, 3912, 4332
BseJI GATNNNNATC 1 cut(s) 765
BseMI GCAATG 3 cut(s) 1928, 2274, 2341
BseRI GAGGAG 7 cut(s) 77, 2225, 2804, 3739, 3868, 4595, 4826
BseSI GKGCMC 2 cut(s) 323, 778
BseXI GCAGC 5 cut(s) 31, 34, 169, 607, 4100
BseYI CCCAGC 1 cut(s) 4931
BsgI GTGCAG 1 cut(s) 246
BshFI GGCC 5 cut(s) 5, 2552, 2579, 4135, 4702
BshNI GGYRCC 1 cut(s) 2030
BsiHKAI GWGCWC 2 cut(s) 1256, 4260
BsiHKCI CYCGRG 3 cut(s) 47, 2207, 4804
BslFI GGGAC 2 cut(s) 785, 4174
BsmFI GGGAC 2 cut(s) 785, 4174
BsmI GAATGC 2 cut(s) 2744, 3874
BsnI GGCC 5 cut(s) 5, 2552, 2579, 4135, 4702
Bso31I GGTCTC 2 cut(s) 44, 4516
BsoBI CYCGRG 3 cut(s) 47, 2207, 4804
Bsp119I TTCGAA 3 cut(s) 967, 1071, 2280
Bsp1286I GDGCHC 6 cut(s) 323, 778, 893, 1256, 3966, 4260
Bsp13I TCCGGA 2 cut(s) 2887, 3861
Bsp1407I TGTACA 3 cut(s) 1153, 2128, 2184
Bsp1720I GCTNAGC 1 cut(s) 3193
Bsp19I CCATGG 2 cut(s) 1209, 4867
BspACI CCGC 3 cut(s) 1142, 2141, 4211
BspANI GGCC 5 cut(s) 5, 2552, 2579, 4135, 4702
BspEI TCCGGA 2 cut(s) 2887, 3861
BspHI TCATGA 4 cut(s) 522, 1123, 3622, 3919
BspLI GGNNCC 4 cut(s) 40, 2032, 3993, 4911
BspMAI CTGCAG 2 cut(s) 1870, 4118
BspMI ACCTGC 1 cut(s) 1513
BspT104I TTCGAA 3 cut(s) 967, 1071, 2280
BspT107I GGYRCC 1 cut(s) 2030
BspTI CTTAAG 1 cut(s) 3353
BspTNI GGTCTC 2 cut(s) 44, 4516
BsrDI GCAATG 3 cut(s) 1928, 2274, 2341
BsrFI RCCGGY 2 cut(s) 1056, 2715
BsrGI TGTACA 3 cut(s) 1153, 2128, 2184
BssAI RCCGGY 2 cut(s) 1056, 2715
BssNAI GTATAC 1 cut(s) 1206
BssT1I CCWWGG 8 cut(s) 66, 144, 1209, 2136, 2418, 2955, 3169, 4867
Bst1107I GTATAC 1 cut(s) 1206
Bst2UI CCWGG 4 cut(s) 1296, 2346, 3912, 4332
Bst6I CTCTTC 8 cut(s) 534, 932, 1068, 1944, 4629, 4671, 4764, 4818
BstAFI CTTAAG 1 cut(s) 3353
BstAPI GCANNNNNTGC 2 cut(s) 2123, 2735
BstAUI TGTACA 3 cut(s) 1153, 2128, 2184
BstBI TTCGAA 3 cut(s) 967, 1071, 2280
BstC8I GCNNGC 9 cut(s) 96, 1221, 1591, 1918, 2229, 2565, 2922, 4277, 4704
BstDSI CCRYGG 2 cut(s) 1209, 4867
BstEII GGTNACC 1 cut(s) 4871
BstH2I RGCGCY 3 cut(s) 1174, 3193, 3792
BstHHI GCGC 5 cut(s) 659, 1173, 2920, 3192, 3791
BstNI CCWGG 4 cut(s) 1296, 2346, 3912, 4332
BstNSI RCATGY 8 cut(s) 237, 989, 1122, 1826, 2135, 3158, 4054, 4199
BstPAI GACNNNNGTC 1 cut(s) 4269
BstPI GGTNACC 1 cut(s) 4871
BstSFI CTRYAG 3 cut(s) 1866, 3084, 4114
BstSLI GKGCMC 2 cut(s) 323, 778
BstV1I GCAGC 5 cut(s) 31, 34, 169, 607, 4100
BstV2I GAAGAC 7 cut(s) 1414, 2116, 2222, 2269, 3908, 3951, 4107
BstX2I RGATCY 8 cut(s) 38, 1334, 1551, 1859, 2322, 3907, 4058, 4936
BstYI RGATCY 8 cut(s) 38, 1334, 1551, 1859, 2322, 3907, 4058, 4936
BstZ17I GTATAC 1 cut(s) 1206
BsuRI GGCC 5 cut(s) 5, 2552, 2579, 4135, 4702
BtgI CCRYGG 2 cut(s) 1209, 4867
BtsI GCAGTG 3 cut(s) 881, 2058, 3141
BveI ACCTGC 1 cut(s) 1513
Cac8I GCNNGC 9 cut(s) 96, 1221, 1591, 1918, 2229, 2565, 2922, 4277, 4704
CaiI CAGNNNCTG 3 cut(s) 3911, 4061, 4838
CciI TCATGA 4 cut(s) 522, 1123, 3622, 3919
CfoI GCGC 5 cut(s) 659, 1173, 2920, 3192, 3791
Cfr10I RCCGGY 2 cut(s) 1056, 2715
Cfr13I GGNCC 9 cut(s) 4, 350, 421, 562, 2551, 2713, 3073, 3562, 3991
CseI GACGC 1 cut(s) 80
CsiI ACCWGGT 2 cut(s) 2344, 4330
CspCI CAANNNNNGTGG 2 cut(s) 4066, 4101
DraI TTTAAA 1 cut(s) 849
DraIII CACNNNGTG 1 cut(s) 4982
DrdI GACNNNNNNGTC 2 cut(s) 77, 4168
DriI GACNNNNNGTC 2 cut(s) 718, 4332
DseDI GACNNNNNNGTC 2 cut(s) 77, 4168
Eam1104I CTCTTC 8 cut(s) 534, 932, 1068, 1944, 4629, 4671, 4764, 4818
Eam1105I GACNNNNNGTC 2 cut(s) 718, 4332
EarI CTCTTC 8 cut(s) 534, 932, 1068, 1944, 4629, 4671, 4764, 4818
Ecl136II GAGCTC 1 cut(s) 4258
Eco130I CCWWGG 8 cut(s) 66, 144, 1209, 2136, 2418, 2955, 3169, 4867
Eco147I AGGCCT 1 cut(s) 4702
Eco24I GRGCYC 3 cut(s) 893, 3966, 4260
Eco31I GGTCTC 2 cut(s) 44, 4516
Eco32I GATATC 3 cut(s) 610, 3550, 4096
Eco47I GGWCC 7 cut(s) 350, 421, 562, 2713, 3073, 3562, 3991
Eco47III AGCGCT 2 cut(s) 3191, 3790
Eco53kI GAGCTC 1 cut(s) 4258
Eco57I CTGAAG 8 cut(s) 1044, 1906, 3138, 3888, 4713, 4749, 4860, 4907
Eco88I CYCGRG 3 cut(s) 47, 2207, 4804
Eco91I GGTNACC 1 cut(s) 4871
EcoICRI GAGCTC 1 cut(s) 4258
EcoO109I RGGNCCY 3 cut(s) 562, 3073, 3991
EcoO65I GGTNACC 1 cut(s) 4871
EcoRI GAATTC 1 cut(s) 3148
EcoRII CCWGG 4 cut(s) 1294, 2344, 3910, 4330
EcoRV GATATC 3 cut(s) 610, 3550, 4096
EcoT14I CCWWGG 8 cut(s) 66, 144, 1209, 2136, 2418, 2955, 3169, 4867
EcoT38I GRGCYC 3 cut(s) 893, 3966, 4260
ErhI CCWWGG 8 cut(s) 66, 144, 1209, 2136, 2418, 2955, 3169, 4867
FalI AAGNNNNNCTT 4 cut(s) 549, 581, 2551, 2583
FaqI GGGAC 2 cut(s) 785, 4174
FbaI TGATCA 3 cut(s) 484, 829, 1639
FblI GTMKAC 2 cut(s) 1205, 2476
Fnu4HI GCNGC 6 cut(s) 20, 23, 158, 621, 2142, 4114
FriOI GRGCYC 3 cut(s) 893, 3966, 4260
Fsp4HI GCNGC 6 cut(s) 20, 23, 158, 621, 2142, 4114
FspBI CTAG 9 cut(s) 224, 624, 1145, 1698, 2286, 2804, 3690, 4238, 4362
FspI TGCGCA 1 cut(s) 658
GlaI GCGC 5 cut(s) 658, 1172, 2919, 3191, 3790
GluI GCNGC 6 cut(s) 20, 23, 158, 621, 2142, 4114
GsaI CCCAGC 1 cut(s) 4935
GsuI CTGGAG 6 cut(s) 117, 401, 834, 1490, 1862, 4728
HaeII RGCGCY 3 cut(s) 1174, 3193, 3792
HaeIII GGCC 5 cut(s) 5, 2552, 2579, 4135, 4702
HgaI GACGC 1 cut(s) 80
HhaI GCGC 5 cut(s) 659, 1173, 2920, 3192, 3791
Hin6I GCGC 5 cut(s) 657, 1171, 2918, 3190, 3789
HinP1I GCGC 5 cut(s) 657, 1171, 2918, 3190, 3789
HincII GTYRAC 5 cut(s) 723, 859, 945, 3225, 4264
HindII GTYRAC 5 cut(s) 723, 859, 945, 3225, 4264
HindIII AAGCTT 4 cut(s) 218, 665, 2970, 4757
HpaI GTTAAC 1 cut(s) 859
Hpy166II GTNNAC 9 cut(s) 723, 859, 945, 1206, 2477, 2632, 3225, 4264, 4588
Hpy8I GTNNAC 9 cut(s) 723, 859, 945, 1206, 2477, 2632, 3225, 4264, 4588
HpyCH4IV ACGT 2 cut(s) 3311, 3889
HpySE526I ACGT 2 cut(s) 3311, 3889
HspAI GCGC 5 cut(s) 657, 1171, 2918, 3190, 3789
Kpn2I TCCGGA 2 cut(s) 2887, 3861
KpnI GGTACC 1 cut(s) 2034
Ksp22I TGATCA 3 cut(s) 484, 829, 1639
KspAI GTTAAC 1 cut(s) 859
LmnI GCTCC 8 cut(s) 90, 277, 1033, 1471, 1549, 1819, 3698, 4255
Lsp1109I GCAGC 5 cut(s) 31, 34, 169, 607, 4100
MabI ACCWGGT 2 cut(s) 2344, 4330
MaeI CTAG 9 cut(s) 224, 624, 1145, 1698, 2286, 2804, 3690, 4238, 4362
MaeII ACGT 2 cut(s) 3311, 3889
MfeI CAATTG 4 cut(s) 1344, 1643, 2541, 2993
MflI RGATCY 8 cut(s) 38, 1334, 1551, 1859, 2322, 3907, 4058, 4936
MhlI GDGCHC 6 cut(s) 323, 778, 893, 1256, 3966, 4260
MlyI GAGTC 7 cut(s) 158, 379, 3331, 3845, 4583, 4661, 4835
MmeI TCCRAC 4 cut(s) 99, 847, 1044, 3024
MroI TCCGGA 2 cut(s) 2887, 3861
MroXI GAANNNNTTC 6 cut(s) 909, 1353, 1626, 1836, 2681, 4230
MslI CAYNNNNRTG 3 cut(s) 1533, 3027, 3310
MspA1I CMGCKG 1 cut(s) 4748
MspCI CTTAAG 1 cut(s) 3353
MunI CAATTG 4 cut(s) 1344, 1643, 2541, 2993
Mva1269I GAATGC 2 cut(s) 2744, 3874
MvaI CCWGG 4 cut(s) 1296, 2346, 3912, 4332
NciI CCSGG 6 cut(s) 253, 888, 2039, 2463, 3566, 3636
NcoI CCATGG 2 cut(s) 1209, 4867
NlaIV GGNNCC 4 cut(s) 40, 2032, 3993, 4911
NmeAIII GCCGAG 1 cut(s) 1192
NmuCI GTSAC 9 cut(s) 126, 442, 1052, 1512, 3164, 3950, 3956, 4324, 4871
NsbI TGCGCA 1 cut(s) 658
NspI RCATGY 8 cut(s) 237, 989, 1122, 1826, 2135, 3158, 4054, 4199
NspV TTCGAA 3 cut(s) 967, 1071, 2280
OliI CACNNNNGTG 1 cut(s) 3310
PaeR7I CTCGAG 3 cut(s) 47, 2207, 4804
PagI TCATGA 4 cut(s) 522, 1123, 3622, 3919
PaqCI CACCTGC 1 cut(s) 1513
PceI AGGCCT 1 cut(s) 4702
PciI ACATGT 2 cut(s) 233, 985
PctI GAATGC 2 cut(s) 2744, 3874
PdmI GAANNNNTTC 6 cut(s) 909, 1353, 1626, 1836, 2681, 4230
PflFI GACNNNGTC 1 cut(s) 3229
PflMI CCANNNNNTGG 1 cut(s) 359
PfoI TCCNGGA 2 cut(s) 2037, 3564
PkrI GCNGC 6 cut(s) 21, 24, 159, 622, 2143, 4115
PleI GAGTC 7 cut(s) 157, 379, 3331, 3844, 4583, 4661, 4834
PpsI GAGTC 7 cut(s) 157, 379, 3331, 3844, 4583, 4661, 4834
PpuMI RGGWCCY 3 cut(s) 562, 3073, 3991
PscI ACATGT 2 cut(s) 233, 985
PshAI GACNNNNGTC 1 cut(s) 4269
PsiI TTATAA 2 cut(s) 2312, 3455
Psp124BI GAGCTC 1 cut(s) 4260
Psp5II RGGWCCY 3 cut(s) 562, 3073, 3991
Psp6I CCWGG 4 cut(s) 1294, 2344, 3910, 4330
PspEI GGTNACC 1 cut(s) 4871
PspFI CCCAGC 1 cut(s) 4931
PspGI CCWGG 4 cut(s) 1294, 2344, 3910, 4330
PspN4I GGNNCC 4 cut(s) 40, 2032, 3993, 4911
PspPI GGNCC 9 cut(s) 4, 350, 421, 562, 2551, 2713, 3073, 3562, 3991
PspPPI RGGWCCY 3 cut(s) 562, 3073, 3991
PspXI VCTCGAGB 1 cut(s) 2207
PsrI GAACNNNNNNTAC 2 cut(s) 4342, 4374
PstI CTGCAG 2 cut(s) 1870, 4118
PstNI CAGNNNCTG 3 cut(s) 3911, 4061, 4838
PsuI RGATCY 8 cut(s) 38, 1334, 1551, 1859, 2322, 3907, 4058, 4936
PsyI GACNNNGTC 1 cut(s) 3229
PvuII CAGCTG 1 cut(s) 4748
RseI CAYNNNNRTG 3 cut(s) 1533, 3027, 3310
SacI GAGCTC 1 cut(s) 4260
SatI GCNGC 6 cut(s) 20, 23, 158, 621, 2142, 4114
Sau96I GGNCC 9 cut(s) 4, 350, 421, 562, 2551, 2713, 3073, 3562, 3991
ScaI AGTACT 1 cut(s) 1780
SchI GAGTC 7 cut(s) 158, 379, 3331, 3845, 4583, 4661, 4835
SduI GDGCHC 6 cut(s) 323, 778, 893, 1256, 3966, 4260
SexAI ACCWGGT 2 cut(s) 2344, 4330
SfcI CTRYAG 3 cut(s) 1866, 3084, 4114
Sfr274I CTCGAG 3 cut(s) 47, 2207, 4804
SfuI TTCGAA 3 cut(s) 967, 1071, 2280
SinI GGWCC 7 cut(s) 350, 421, 562, 2713, 3073, 3562, 3991
SlaI CTCGAG 3 cut(s) 47, 2207, 4804
SmiMI CAYNNNNRTG 3 cut(s) 1533, 3027, 3310
SseBI AGGCCT 1 cut(s) 4702
SsiI CCGC 3 cut(s) 1142, 2141, 4211
SspI AATATT 5 cut(s) 1900, 2487, 2944, 4495, 4660
SspMI CTAG 9 cut(s) 224, 624, 1145, 1698, 2286, 2804, 3690, 4238, 4362
SstI GAGCTC 1 cut(s) 4260
StuI AGGCCT 1 cut(s) 4702
StyI CCWWGG 8 cut(s) 66, 144, 1209, 2136, 2418, 2955, 3169, 4867
TaiI ACGT 2 cut(s) 3314, 3892
TauI GCSGC 1 cut(s) 2144
TseFI GTSAC 9 cut(s) 126, 442, 1052, 1512, 3164, 3950, 3956, 4324, 4871
TseI GCWGC 5 cut(s) 19, 22, 157, 620, 4113
Tsp45I GTSAC 9 cut(s) 126, 442, 1052, 1512, 3164, 3950, 3956, 4324, 4871
TspGWI ACGGA 4 cut(s) 1777, 2576, 3736, 4970
Tth111I GACNNNGTC 1 cut(s) 3229
Van91I CCANNNNNTGG 1 cut(s) 359
Vha464I CTTAAG 1 cut(s) 3353
VpaK11BI GGWCC 7 cut(s) 350, 421, 562, 2713, 3073, 3562, 3991
XceI RCATGY 8 cut(s) 237, 989, 1122, 1826, 2135, 3158, 4054, 4199
XcmI CCANNNNNNNNNTGG 1 cut(s) 1216
XhoI CTCGAG 3 cut(s) 47, 2207, 4804
XmiI GTMKAC 2 cut(s) 1205, 2476
XmnI GAANNNNTTC 6 cut(s) 909, 1353, 1626, 1836, 2681, 4230
XspI CTAG 9 cut(s) 224, 624, 1145, 1698, 2286, 2804, 3690, 4238, 4362
ZrmI AGTACT 1 cut(s) 1780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.