MD05G1361800.v1.1

Sister chromatid cohesion protein PDS5 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009376
Physical Location & Seq
Forward (+)
47702964 .. 47716168
13205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1361800.v1.1.491

Sequence Viewer

Length: 5013 bp
ATGGCCCAAAAGCTCGAGCAGCTGCTCAAGGAGGTGGGATCGAAGCTTGAGACCCCTCATTCTTCCAAAGACGCTCTCGTCAAGCTCTTAAAGCAAGCTGCATCATGTCTTTCGGACTTGGATCAAGCACCTCCAGCGTCTATGTTGGAGTCAATGCAGCCTTTGCTTAATGCAATTGTCAAGCCAGAACTGTTGAAGCATCAAGATAGAGACGTCAAGCTTCTAGTTGCAACTTGTATTTGTGAGATAACTCGGATTACTGCACCTGAAGCTCCTTATAGTGATGAAGTGCTAAAGGATATATTTCACTTGATTGTGGGAACTTTTAGTGGATTGAAAGATACAAGTGGTCCATCATTTGGTAGAAGAGTACTCATTTTGGAGACTCTTGCCAAATATAGGTCATGTGTTGTAATGTTGGACCTCGAATGTGATGATCTGGTGAATGAAATGTTCAGTACATTTTTTGCAGTTGCCAGAGATGATCACCAAGAAACTGTTTTGTCATCGATGCAAACAATCATGGTTGTGCTCTTAGAAGAGAGCGAGGAATTGCGAGATGATCTTTTGTTTATTGTATTGTCTATATTAGGTCGTAATAGAAGTGATATCACTGTGGCTGCTAGGAGGCTTGCCATGAATGTTATAGAACAGTGCGCAGGAAAGCTTGAATCTGGCATTAAACAATTTCTTATATCATCAATGTCAGGAGACAACAAGTCTGAGAATCATCAGATTGATTACCATGAAGTCATCTATGATGTATATCGTTCTGCTCCCCAAATCTTATCAGGAGTTGTCCCATACCTAACAGGAGAGTTACTGACTGATCAGCTAGAAACTCGTTTAAAAGCCGTTAGTTTAGTTGGAGACCTATTCTCTCTGCCAGGCTCTACCATCTTTGAAGCCTTTCAGCCAATCTTTTCAGAGTTCTTGAAGAGGTTGACTGATAGAGTTGTGGAGGTTCGCATGTTGGTCCTCCAACATGTCAAGAGCTGTATGCTGTCAAATCCTTTCAGAGCTGAAGCTCCCGAAATAATTTCTGCCCTCTGTGACCGGCTATTGGACTTTGAAGAAACAGTACGAAAGCAAGTTGTTGCTGTAGTTTATGATGTGGCATGTCATGCCCTGAGTTCCATTCCGCTAGAAACTATAAAACTGGTTGCAGAGCGCCTTCGAGATAAATCTCTACTTGTTAAAAAGTATACCATGGAGAGGTTAGCTGAGATATTCAGGGTTTATTGCACAATGTGCTCTGATGGTCCACTCCTATCCAGTGATTTTGATTGGATTCCTGGGAAGATTCTGAGATGTTTTTATGACAAAGATTTCAGATCTGATACAATTGAAAGTGTTCTCTGTGAGTCCCTGTTCCCAACTAACTTTTCAGTAAAAGATAAAGTTAAACATTGGGTGAGAGTCTTCTCAAGATTTGATAAAGTTGAGGTTAAGGCTCTTGAGAAGATTCTGGAGCAGAAGCAGAGGTTACAACAAGAGATGCAGAAGTATCTGGCACTTAAGCAGATGCATCAGGATGGTGATGCTCCTGAGGTCCAAAAAAAAATTCTCTATTGCTTCCGTATAATGTCACGGTTGTTTGCTGATCCAGTTAAGGCTGAAGAGAGTTTTCAATTTCTTGATCAACTGAAAGACGTTAACATCTGGAAGATCTTAACAAATCTTGTTGATCCAAACACTGGCTCCCAGCAAGCTTGCACCTTACGGGATGATTTGCTTAGGATTCTAGGTGAAAAACACCGACTATATGATTTTCTTAGCACTTTCTCAGTGAAGTGTTCTTATCTACTTTTCAACAAAGAGCATGTAAAGGAAATTCTTCTGGAGGTTCTTGTACACAAATCTACCGCAGATATGAAATATGTGCAATCTTGCATGAATATTTTGGTGATTCTTGCACGCTTTAGTCCATTACTACTCAGTGGGACAGGAGAAGAACTAGTAAATTTGCTTAAAGACGATGATGAAACCATAAAAGAAGGGGTTTTGAATATTTTAGCAAAGGCTGGTGGTACCATCCGGGAGCATCTTGCAGTGTCATCAAGTTCCATAGACCTTGTATTGGAGAGGTTGTGTTTGGAGGGCAGTCGAAGACAGGCAAAGTATGCTGTACATGCCCTCGCAGCGATAACAAAGGATGATGGACTTAAATCACTCTCTGTTCTATACAAGAGGCTTGTGGATATGCTGGAGGAGAGGACACATTTGCCTGCTGTACTACAATCTCTGGGGTGTATAGCTCAGACTGCAATGCCAGTTTTTGAAACTAGAGAGAAGGAAATTGAAAAGTTTATACTTGAAAATATCCTAAAGTGCGATAATAAATCAGGTGACTATAAAATTGCATCCTGGGATGACAAAAGTGATCTTTGTTTGCTAAAGATATATGGGATAAAGACATTAGTGAAAAGTTATTTGCCTGTCAAAGATGCTCATGTTCGCTCTGGCATTGATGGTCTGCTGGAAATCTTAAGAAACACACTTTCTTGTGGAGAAATATCAAAAGACATAGAAACAAGTTCAGTTGACAAGGCGCATTTGAGGCTTGCTTCTGCAAAGGCGATTCTTCGTCTGTCCAAGCACTGGAATCATAAGATACCGGTTGATATCTTCTACTTAACCTTGAAGACTTCAGAGATTAGTTTCCCTCAAGCTAAGAAAATTTTCCTGAACAAAGTTCATCAATACATAAAGGACCGGCTTTTAGATGGAAAATACGCTTGTGCATTCTTTTTTAACACATCTGGATCAAAGTCAGTGGAGTTTCAAGAGGAGAAACAGAATCTCGCAGACATTATTCAAATGTATCACCAAACAAAGGGGCGGCATCTCTCCATGCAGCAATCTGATGCCAATTCATTGACTGCTTATCCGGAGTACATCCTCCCATACTTGGTTCATGCCCTTGCACATCATTCTTGCCCTAATATTGATGAATGCAAGGATGTGAAAGCATTTGAGCCGATATACCGGCAATTGCACTTATTTCTGTCTATGTTGGTGCATAGAGATGATGATGTCAAGTCAGAATCTAGTAGCAACATTGAAAAGGAGGATATTTCTGCTATAATCTCTATCTTTCAAAGTATCAAGTGCTCTGAAGATATATATGATGCAACAAAGTCAAAGAATTCACATGGTATTTGTGACCTTGGGCTGTCAGTTACCAAGCGCTTATCCCCAAAGGAGTATGATTTACAAGGATTGCCTGCATCAGTTCCTCTACCTTCTATGTTGTACAAACAGTATGACAAGAAAGAAGGAGATGACTCTATGGCCAGTGAAGGGAAAACATGGTTGGCCGATGACTCTGCGGTGGCTCACTTTGAATCTCTTAAGTTAGAGTCTAGTGAGACGGGACTTTCAGAGATTGCTGAAGATGAGCTTATGAAAGATGGTGAAAGAGATGGAGGTGAAGTGCCTTTAGGAAGAATTATAAAGCGCTTAAGATCTCAAAATTCCAAGGCTAAAAAGGCAAAAAAGAACAAGGCATCATCAGCTGATGCAGAGAATGCTGAAAATAGTGTTGATATATTGAAAATGGTCAGGGATATAAATTTGGATAATTTGGAAAAGCCTTCTAAGTTTGAGTCAAGCAACGGTCATGAAAATCTTCCAAGCAAGAAATCAAGAATGGATCTAAAGCACAAAAAGGAAAACAAAAGAAAATCTACTGAGGCAACGTCTGTCCCCATTCCTAAACGTAGGAGGTCATCATCCGCTCATGGTGCTTTCAGATCACCTAGAAGTACTTCAAAGTCTCCTCTAAGTGCTTCAGTAGACGATGTAGGCAATAGAAAACTGGTTCAAGGCACCGAGTCAGATTTGTTGGTTTCATGCTTCCGGAAGAATGCTACCTCCTCGTCACAACGTAAAGGCAGAGCCTCTGACCATGGTCTTAATGATGAGACAAATGAAGTTGGAGAAGCCAGTGACCGTGAAGAGCCTAATGTGCTGGAGGCTGACAAGGATGACCTAAATAGTGATTTCAACTCTCCTGCAGGATCCATCAAGAAGCGGAAAAGAAAGAGCATTTCCGGTTTGGCAAAGTGCAAATTCAAGGAAAGTGGAAAAGATGTCGAAGACTTGATTGGTTGCAGAATAAAAGTTTGGTGGCCAATGGATAAGCGATTTTATGAAGGCACGGTGAAGTCGTTTGATACGTTAAAGAGGAAGCATGTGATACTGTATGAAGATGGAGATGTAGAAGTACTTCGGTTAGAAAAGGAACGGTGGGAGCTCATTGACAAAGGTCGCAAGCCCACAAAGAAGTTAAATTCGTCAAATAAATCCCCCTCAAAAGAAGTGTCCCCTGGTCAAAAGAATAAGCCCACAGGTGGTTCGCGAGAGAACAAAAAACCAATTAAAACAGTTAAAAGGAAAAGAACACCAAAGAAAAACTCGGCCAGGAAGAAGGGCGTATCAAAAAGAAATCATTGGGGAAGTAGGGACAAAGAGAGTTCTGAAGTATCGAATGATGAGCCTGCCCTTACATCTAAAATTGATGAAATCGACTCTGGTAGTTCAGAAGAAGAGAAAGCTGAAAAAGTGGACGAGAACGTGACAGATGAGGGGGAATCTGACACGGAAGTGAAGTTTGTTTCTGAAGGAAAAGGGTTGAAAGATGCGGAAGAAAGTCCAAATAATACCGAGGAATCTGATGAAGAGAATCCTGGTTCGGAAGGGATCCCTGCTGAAGACATGGACAGTATTCCACAAGATGCTGAAAATGGAACTGAGGAGATGCAACACTCAGAAGAGAAAGAAGCAGATGAACTAAGTGGAGGGTCAAGAGGGGTTAATGAGGAAGATCCGTCAGATTCTGAAGGGACTCAAGATAAAGATGATATTTCTGGTAGTCCCTTGAAGCAGGAGAAATCCCATGTAGAGCCTTCAAGTCTTTCTGATGCCGGAGATGACGAACTTTCTGATGATGAGCCTCTCGGCAAGTGGACGCAACGAGTAGTGAAAAAAGGTTCAAAGCGAGTGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1671

Amino Acids

187.81

Weight (kDa)

5.94

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 24 - 1087 7.5e-260 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3455
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 658
Acc65I GGTACC 1 cut(s) 2030
AccB1I GGYRCC 2 cut(s) 2030, 3830
AccB7I CCANNNNNTGG 3 cut(s) 359, 1697, 2601
AccBSI CCGCTC 1 cut(s) 3740
AccI GTMKAC 2 cut(s) 1205, 3798
AccII CGCG 1 cut(s) 4363
AccIII TCCGGA 2 cut(s) 2890, 3861
AciI CCGC 7 cut(s) 1142, 1866, 2842, 3332, 3738, 4036, 4646
AcoI YGGCCR 4 cut(s) 3294, 3318, 4133, 4422
AcsI RAATTY 9 cut(s) 1563, 1833, 1963, 2679, 3148, 3475, 3574, 4073, 4294
AcyI GRCGYC 1 cut(s) 213
AdeI CACNNNGTG 1 cut(s) 1251
AfeI AGCGCT 2 cut(s) 3191, 3461
AflII CTTAAG 4 cut(s) 1517, 2488, 3353, 3463
AflIII ACRYGT 1 cut(s) 985
AgeI ACCGGT 1 cut(s) 2617
AhdI GACNNNNNGTC 1 cut(s) 718
AhlI ACTAGT 1 cut(s) 1957
AjnI CCWGG 6 cut(s) 886, 1294, 2366, 4330, 4424, 4690
AleI CACNNNNGTG 1 cut(s) 4607
Alw21I GWGCWC 4 cut(s) 534, 1256, 3116, 4260
Alw26I GTCTC 8 cut(s) 44, 204, 377, 705, 864, 3365, 3783, 3920
AlwNI CAGNNNCTG 1 cut(s) 4841
Ama87I CYCGRG 1 cut(s) 14
Aor13HI TCCGGA 2 cut(s) 2890, 3861
Aor51HI AGCGCT 2 cut(s) 3191, 3461
AoxI GGCC 5 cut(s) 3, 3294, 3318, 4133, 4422
ApeKI GCWGC 7 cut(s) 19, 22, 98, 157, 620, 2141, 2857
ApoI RAATTY 9 cut(s) 1563, 1833, 1963, 2679, 3148, 3475, 3574, 4073, 4294
ArsI GACNNNNNNTTYG 2 cut(s) 4310, 4342
AsiGI ACCGGT 1 cut(s) 2617
Asp700I GAANNNNTTC 7 cut(s) 909, 1353, 1836, 2681, 3630, 3769, 4230
Asp718I GGTACC 1 cut(s) 2030
AspLEI GCGC 5 cut(s) 659, 1173, 2554, 3192, 3462
AspS9I GGNCC 7 cut(s) 4, 350, 421, 976, 1262, 1552, 2713
AsuC2I CCSGG 1 cut(s) 2039
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 6 cut(s) 350, 421, 976, 1262, 1552, 2713
AxyI CCTNAGG 1 cut(s) 1548
BalI TGGCCA 2 cut(s) 3296, 4135
BamHI GGATCC 2 cut(s) 4022, 4704
BanI GGYRCC 2 cut(s) 2030, 3830
BanII GRGCYC 1 cut(s) 4260
BarI GAAGNNNNNNTAC 4 cut(s) 1065, 1097, 4215, 4247
BbsI GAAGAC 5 cut(s) 1414, 2116, 2651, 4107, 4722
Bbv12I GWGCWC 4 cut(s) 534, 1256, 3116, 4260
BbvI GCAGC 7 cut(s) 9, 31, 85, 169, 607, 2153, 2869
BceAI ACGGC 1 cut(s) 839
BcgI CGANNNNNNTGC 2 cut(s) 3311, 3345
BciT130I CCWGG 6 cut(s) 888, 1296, 2368, 4332, 4426, 4692
BclI TGATCA 3 cut(s) 484, 829, 1639
BcnI CCSGG 1 cut(s) 2039
BcoDI GTCTC 8 cut(s) 44, 204, 377, 705, 864, 3365, 3783, 3920
BcuI ACTAGT 1 cut(s) 1957
BfmI CTRYAG 2 cut(s) 1101, 4017
BfoI RGCGCY 3 cut(s) 1174, 3193, 3463
BfrI CTTAAG 4 cut(s) 1517, 2488, 3353, 3463
BglII AGATCT 3 cut(s) 1334, 1668, 3467
BisI GCNGC 8 cut(s) 20, 23, 99, 158, 621, 2142, 2843, 2858
BlsI GCNGC 8 cut(s) 21, 24, 100, 159, 622, 2143, 2844, 2859
BmcAI AGTACT 3 cut(s) 372, 3769, 4230
Bme1390I CCNGG 7 cut(s) 888, 1296, 2039, 2368, 4332, 4426, 4692
Bme18I GGWCC 6 cut(s) 350, 421, 976, 1262, 1552, 2713
BmeRI GACNNNNNGTC 1 cut(s) 718
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 7 cut(s) 4, 350, 421, 976, 1262, 1552, 2713
BmiI GGNNCC 5 cut(s) 1702, 2032, 3832, 4024, 4706
BmrFI CCNGG 7 cut(s) 888, 1296, 2039, 2368, 4332, 4426, 4692
BoxI GACNNNNGTC 2 cut(s) 3912, 4269
BpiI GAAGAC 5 cut(s) 1414, 2116, 2651, 4107, 4722
BpmI CTGGAG 5 cut(s) 117, 1490, 1862, 2228, 3995
Bpu10I CCTNAGC 1 cut(s) 1736
BpuEI CTTGAG 6 cut(s) 11, 68, 1411, 1478, 2652, 4836
BpuMI CCSGG 1 cut(s) 2039
Bsa29I ATCGAT 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 765
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 44, 864
BsaJI CCNNGG 8 cut(s) 1209, 1295, 2367, 3169, 3480, 3910, 4330, 4668
BsaWI WCCGGW 4 cut(s) 2617, 2890, 3861, 4055
BsaXI ACNNNNNCTCC 6 cut(s) 1685, 1715, 2075, 2105, 3420, 3450
Bse118I RCCGGY 4 cut(s) 1056, 2617, 2715, 2988
Bse1I ACTGG 9 cut(s) 1164, 1275, 1607, 1702, 2273, 2606, 3297, 3825, 3948
Bse21I CCTNAGG 1 cut(s) 1548
Bse3DI GCAATG 1 cut(s) 2274
Bse8I GATNNNNATC 1 cut(s) 765
BseAI TCCGGA 2 cut(s) 2890, 3861
BseBI CCWGG 6 cut(s) 888, 1296, 2368, 4332, 4426, 4692
BseCI ATCGAT 1 cut(s) 509
BseDI CCNNGG 8 cut(s) 1209, 1295, 2367, 3169, 3480, 3910, 4330, 4668
BseJI GATNNNNATC 1 cut(s) 765
BseMI GCAATG 1 cut(s) 2274
BseNI ACTGG 9 cut(s) 1164, 1275, 1607, 1702, 2273, 2606, 3297, 3825, 3948
BseRI GAGGAG 5 cut(s) 2225, 2804, 3771, 3868, 4772
BseXI GCAGC 7 cut(s) 9, 31, 85, 169, 607, 2153, 2869
BseYI CCCAGC 1 cut(s) 1704
BsgI GTGCAG 1 cut(s) 246
Bsh1236I CGCG 1 cut(s) 4363
BshFI GGCC 5 cut(s) 5, 3296, 3320, 4135, 4424
BshNI GGYRCC 2 cut(s) 2030, 3830
BshTI ACCGGT 1 cut(s) 2617
BshVI ATCGAT 1 cut(s) 509
BsiHKAI GWGCWC 4 cut(s) 534, 1256, 3116, 4260
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 9 cut(s) 1057, 2038, 2618, 2716, 2891, 2989, 3862, 4056, 4929
BslFI GGGAC 9 cut(s) 785, 1351, 1957, 3390, 3692, 4312, 4481, 4861, 4863
BsmAI GTCTC 8 cut(s) 44, 204, 377, 705, 864, 3365, 3783, 3920
BsmBI CGTCTC 2 cut(s) 204, 3365
BsmFI GGGAC 9 cut(s) 785, 1351, 1957, 3390, 3692, 4312, 4481, 4861, 4863
BsmI GAATGC 4 cut(s) 2744, 2960, 3535, 3874
BsnI GGCC 5 cut(s) 5, 3296, 3320, 4135, 4424
Bso31I GGTCTC 2 cut(s) 44, 864
BsoBI CYCGRG 1 cut(s) 14
Bsp1286I GDGCHC 4 cut(s) 534, 1256, 3116, 4260
Bsp13I TCCGGA 2 cut(s) 2890, 3861
Bsp1407I TGTACA 3 cut(s) 1852, 2128, 3255
Bsp19I CCATGG 2 cut(s) 1209, 3910
Bsp68I TCGCGA 1 cut(s) 4363
BspACI CCGC 7 cut(s) 1142, 1866, 2842, 3332, 3738, 4036, 4646
BspANI GGCC 5 cut(s) 5, 3296, 3320, 4135, 4424
BspDI ATCGAT 1 cut(s) 509
BspEI TCCGGA 2 cut(s) 2890, 3861
BspFNI CGCG 1 cut(s) 4363
BspHI TCATGA 1 cut(s) 3622
BspLI GGNNCC 5 cut(s) 1702, 2032, 3832, 4024, 4706
BspMAI CTGCAG 1 cut(s) 4021
BspQI GCTCTTC 1 cut(s) 3954
BspT107I GGYRCC 2 cut(s) 2030, 3830
BspTI CTTAAG 4 cut(s) 1517, 2488, 3353, 3463
BspTNI GGTCTC 2 cut(s) 44, 864
BsrBI CCGCTC 1 cut(s) 3740
BsrDI GCAATG 1 cut(s) 2274
BsrFI RCCGGY 4 cut(s) 1056, 2617, 2715, 2988
BsrGI TGTACA 3 cut(s) 1852, 2128, 3255
BsrI ACTGG 9 cut(s) 1164, 1275, 1607, 1702, 2273, 2606, 3297, 3825, 3948
BssAI RCCGGY 4 cut(s) 1056, 2617, 2715, 2988
BssECI CCNNGG 8 cut(s) 1209, 1295, 2367, 3169, 3480, 3910, 4330, 4668
BssNAI GTATAC 1 cut(s) 1206
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 4 cut(s) 1209, 3169, 3480, 3910
Bst1107I GTATAC 1 cut(s) 1206
Bst2UI CCWGG 6 cut(s) 888, 1296, 2368, 4332, 4426, 4692
Bst6I CTCTTC 8 cut(s) 361, 534, 932, 1614, 3954, 4545, 4677, 4770
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 4 cut(s) 1517, 2488, 3353, 3463
BstAPI GCANNNNNTGC 5 cut(s) 163, 1124, 1251, 2123, 3530
BstAUI TGTACA 3 cut(s) 1852, 2128, 3255
BstDSI CCRYGG 2 cut(s) 1209, 3910
BstFNI CGCG 1 cut(s) 4363
BstH2I RGCGCY 3 cut(s) 1174, 3193, 3463
BstHHI GCGC 5 cut(s) 659, 1173, 2554, 3192, 3462
BstMAI GTCTC 8 cut(s) 44, 204, 377, 705, 864, 3365, 3783, 3920
BstNI CCWGG 6 cut(s) 888, 1296, 2368, 4332, 4426, 4692
BstNSI RCATGY 6 cut(s) 973, 989, 1122, 1826, 2135, 4199
BstPAI GACNNNNGTC 2 cut(s) 3912, 4269
BstSCI CCNGG 7 cut(s) 886, 1294, 2037, 2366, 4330, 4424, 4690
BstSFI CTRYAG 2 cut(s) 1101, 4017
BstUI CGCG 1 cut(s) 4363
BstV1I GCAGC 7 cut(s) 9, 31, 85, 169, 607, 2153, 2869
BstV2I GAAGAC 5 cut(s) 1414, 2116, 2651, 4107, 4722
BstX2I RGATCY 7 cut(s) 1334, 1668, 3467, 3655, 4022, 4704, 4828
BstYI RGATCY 7 cut(s) 1334, 1668, 3467, 3655, 4022, 4704, 4828
BstZ17I GTATAC 1 cut(s) 1206
Bsu15I ATCGAT 1 cut(s) 509
Bsu36I CCTNAGG 1 cut(s) 1548
BsuRI GGCC 5 cut(s) 5, 3296, 3320, 4135, 4424
BsuTUI ATCGAT 1 cut(s) 509
BtgI CCRYGG 2 cut(s) 1209, 3910
BtsI GCAGTG 1 cut(s) 2058
BtuMI TCGCGA 1 cut(s) 4363
CaiI CAGNNNCTG 1 cut(s) 4841
CciI TCATGA 1 cut(s) 3622
CfoI GCGC 5 cut(s) 659, 1173, 2554, 3192, 3462
Cfr10I RCCGGY 4 cut(s) 1056, 2617, 2715, 2988
Cfr13I GGNCC 7 cut(s) 4, 350, 421, 976, 1262, 1552, 2713
ClaI ATCGAT 1 cut(s) 509
CseI GACGC 3 cut(s) 80, 126, 4981
CspAI ACCGGT 1 cut(s) 2617
CspCI CAANNNNNGTGG 4 cut(s) 2757, 2792, 4066, 4101
DraI TTTAAA 1 cut(s) 849
DraIII CACNNNGTG 1 cut(s) 1251
DrdI GACNNNNNNGTC 1 cut(s) 77
DriI GACNNNNNGTC 1 cut(s) 718
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 4 cut(s) 3294, 3318, 4133, 4422
Eam1104I CTCTTC 8 cut(s) 361, 534, 932, 1614, 3954, 4545, 4677, 4770
Eam1105I GACNNNNNGTC 1 cut(s) 718
EarI CTCTTC 8 cut(s) 361, 534, 932, 1614, 3954, 4545, 4677, 4770
Ecl136II GAGCTC 1 cut(s) 4258
Eco130I CCWWGG 4 cut(s) 1209, 3169, 3480, 3910
Eco24I GRGCYC 1 cut(s) 4260
Eco31I GGTCTC 2 cut(s) 44, 864
Eco32I GATATC 2 cut(s) 610, 2626
Eco47I GGWCC 6 cut(s) 350, 421, 976, 1262, 1552, 2713
Eco47III AGCGCT 2 cut(s) 3191, 3461
Eco53kI GAGCTC 1 cut(s) 4258
Eco81I CCTNAGG 1 cut(s) 1548
Eco88I CYCGRG 1 cut(s) 14
EcoICRI GAGCTC 1 cut(s) 4258
EcoRI GAATTC 1 cut(s) 3148
EcoRII CCWGG 6 cut(s) 886, 1294, 2366, 4330, 4424, 4690
EcoRV GATATC 2 cut(s) 610, 2626
EcoT14I CCWWGG 4 cut(s) 1209, 3169, 3480, 3910
EcoT22I ATGCAT 1 cut(s) 1530
EcoT38I GRGCYC 1 cut(s) 4260
ErhI CCWWGG 4 cut(s) 1209, 3169, 3480, 3910
Esp3I CGTCTC 2 cut(s) 204, 3365
FalI AAGNNNNNCTT 2 cut(s) 3387, 3419
FaqI GGGAC 9 cut(s) 785, 1351, 1957, 3390, 3692, 4312, 4481, 4861, 4863
FbaI TGATCA 3 cut(s) 484, 829, 1639
FblI GTMKAC 2 cut(s) 1205, 3798
Fnu4HI GCNGC 8 cut(s) 20, 23, 99, 158, 621, 2142, 2843, 2858
FriOI GRGCYC 1 cut(s) 4260
Fsp4HI GCNGC 8 cut(s) 20, 23, 99, 158, 621, 2142, 2843, 2858
FspI TGCGCA 1 cut(s) 658
GlaI GCGC 5 cut(s) 658, 1172, 2553, 3191, 3461
GluI GCNGC 8 cut(s) 20, 23, 99, 158, 621, 2142, 2843, 2858
GsaI CCCAGC 1 cut(s) 1708
GsuI CTGGAG 5 cut(s) 117, 1490, 1862, 2228, 3995
HaeII RGCGCY 3 cut(s) 1174, 3193, 3463
HaeIII GGCC 5 cut(s) 5, 3296, 3320, 4135, 4424
HapII CCGG 9 cut(s) 1057, 2038, 2618, 2716, 2891, 2989, 3862, 4056, 4929
HgaI GACGC 3 cut(s) 80, 126, 4981
HhaI GCGC 5 cut(s) 659, 1173, 2554, 3192, 3462
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 5 cut(s) 657, 1171, 2552, 3190, 3460
HinP1I GCGC 5 cut(s) 657, 1171, 2552, 3190, 3460
HincII GTYRAC 3 cut(s) 945, 1657, 2545
HindII GTYRAC 3 cut(s) 945, 1657, 2545
HindIII AAGCTT 4 cut(s) 44, 218, 665, 1710
HpaI GTTAAC 1 cut(s) 1657
HpaII CCGG 9 cut(s) 1057, 2038, 2618, 2716, 2891, 2989, 3862, 4056, 4929
Hpy166II GTNNAC 9 cut(s) 945, 1206, 1265, 1657, 1855, 2545, 3799, 4570, 4971
Hpy8I GTNNAC 9 cut(s) 945, 1206, 1265, 1657, 1855, 2545, 3799, 4570, 4971
HpyCH4IV ACGT 7 cut(s) 213, 1653, 3701, 3721, 3889, 4181, 4578
HpySE526I ACGT 7 cut(s) 213, 1653, 3701, 3721, 3889, 4181, 4578
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 5 cut(s) 657, 1171, 2552, 3190, 3460
Kpn2I TCCGGA 2 cut(s) 2890, 3861
KpnI GGTACC 1 cut(s) 2034
Ksp22I TGATCA 3 cut(s) 484, 829, 1639
KspAI GTTAAC 1 cut(s) 1657
LguI GCTCTTC 1 cut(s) 3954
LmnI GCTCC 8 cut(s) 277, 781, 1033, 1471, 1549, 1706, 2041, 4255
Lsp1109I GCAGC 7 cut(s) 9, 31, 85, 169, 607, 2153, 2869
MaeII ACGT 7 cut(s) 213, 1653, 3701, 3721, 3889, 4181, 4578
MbiI CCGCTC 1 cut(s) 3740
MfeI CAATTG 3 cut(s) 174, 1344, 2993
MflI RGATCY 7 cut(s) 1334, 1668, 3467, 3655, 4022, 4704, 4828
MhlI GDGCHC 4 cut(s) 534, 1256, 3116, 4260
MlsI TGGCCA 2 cut(s) 3296, 4135
MluNI TGGCCA 2 cut(s) 3296, 4135
MmeI TCCRAC 5 cut(s) 126, 399, 847, 1006, 3919
Mox20I TGGCCA 2 cut(s) 3296, 4135
Mph1103I ATGCAT 1 cut(s) 1530
MroI TCCGGA 2 cut(s) 2890, 3861
MroXI GAANNNNTTC 7 cut(s) 909, 1353, 1836, 2681, 3630, 3769, 4230
MscI TGGCCA 2 cut(s) 3296, 4135
MslI CAYNNNNRTG 4 cut(s) 527, 1533, 3027, 4607
Msp20I TGGCCA 2 cut(s) 3296, 4135
MspA1I CMGCKG 2 cut(s) 22, 3518
MspCI CTTAAG 4 cut(s) 1517, 2488, 3353, 3463
MspI CCGG 9 cut(s) 1057, 2038, 2618, 2716, 2891, 2989, 3862, 4056, 4929
MspR9I CCNGG 7 cut(s) 888, 1296, 2039, 2368, 4332, 4426, 4692
MunI CAATTG 3 cut(s) 174, 1344, 2993
Mva1269I GAATGC 4 cut(s) 2744, 2960, 3535, 3874
MvaI CCWGG 6 cut(s) 888, 1296, 2368, 4332, 4426, 4692
MvnI CGCG 1 cut(s) 4363
NciI CCSGG 1 cut(s) 2039
NcoI CCATGG 2 cut(s) 1209, 3910
NlaIV GGNNCC 5 cut(s) 1702, 2032, 3832, 4024, 4706
NmeAIII GCCGAG 2 cut(s) 4400, 4941
NmuCI GTSAC 7 cut(s) 1052, 1587, 2348, 3164, 3882, 3950, 4579
NruI TCGCGA 1 cut(s) 4363
NsbI TGCGCA 1 cut(s) 658
NsiI ATGCAT 1 cut(s) 1530
NspI RCATGY 6 cut(s) 973, 989, 1122, 1826, 2135, 4199
OliI CACNNNNGTG 1 cut(s) 4607
PaeR7I CTCGAG 1 cut(s) 14
PagI TCATGA 1 cut(s) 3622
PciI ACATGT 1 cut(s) 985
PciSI GCTCTTC 1 cut(s) 3954
PcsI WCGNNNNNNNCGW 2 cut(s) 4169, 4178
PctI GAATGC 4 cut(s) 2744, 2960, 3535, 3874
PdmI GAANNNNTTC 7 cut(s) 909, 1353, 1836, 2681, 3630, 3769, 4230
PflMI CCANNNNNTGG 3 cut(s) 359, 1697, 2601
PfoI TCCNGGA 1 cut(s) 2037
PinAI ACCGGT 1 cut(s) 2617
PkrI GCNGC 8 cut(s) 21, 24, 100, 159, 622, 2143, 2844, 2859
PscI ACATGT 1 cut(s) 985
PshAI GACNNNNGTC 2 cut(s) 3912, 4269
PsiI TTATAA 1 cut(s) 3455
Psp124BI GAGCTC 1 cut(s) 4260
Psp6I CCWGG 6 cut(s) 886, 1294, 2366, 4330, 4424, 4690
PspFI CCCAGC 1 cut(s) 1704
PspGI CCWGG 6 cut(s) 886, 1294, 2366, 4330, 4424, 4690
PspN4I GGNNCC 5 cut(s) 1702, 2032, 3832, 4024, 4706
PspPI GGNCC 7 cut(s) 4, 350, 421, 976, 1262, 1552, 2713
PspXI VCTCGAGB 1 cut(s) 14
PstI CTGCAG 1 cut(s) 4021
PstNI CAGNNNCTG 1 cut(s) 4841
PsuI RGATCY 7 cut(s) 1334, 1668, 3467, 3655, 4022, 4704, 4828
PvuII CAGCTG 2 cut(s) 22, 3518
RruI TCGCGA 1 cut(s) 4363
RseI CAYNNNNRTG 4 cut(s) 527, 1533, 3027, 4607
SacI GAGCTC 1 cut(s) 4260
SapI GCTCTTC 1 cut(s) 3954
SatI GCNGC 8 cut(s) 20, 23, 99, 158, 621, 2142, 2843, 2858
Sau96I GGNCC 7 cut(s) 4, 350, 421, 976, 1262, 1552, 2713
SbfI CCTGCAGG 1 cut(s) 4021
ScaI AGTACT 3 cut(s) 372, 3769, 4230
ScrFI CCNGG 7 cut(s) 888, 1296, 2039, 2368, 4332, 4426, 4692
SdaI CCTGCAGG 1 cut(s) 4021
SduI GDGCHC 4 cut(s) 534, 1256, 3116, 4260
SfcI CTRYAG 2 cut(s) 1101, 4017
Sfr274I CTCGAG 1 cut(s) 14
SinI GGWCC 6 cut(s) 350, 421, 976, 1262, 1552, 2713
SlaI CTCGAG 1 cut(s) 14
SmiMI CAYNNNNRTG 4 cut(s) 527, 1533, 3027, 4607
SpeI ACTAGT 1 cut(s) 1957
Sse8387I CCTGCAGG 1 cut(s) 4021
SsiI CCGC 7 cut(s) 1142, 1866, 2842, 3332, 3738, 4036, 4646
SspI AATATT 3 cut(s) 1900, 2011, 2947
SstI GAGCTC 1 cut(s) 4260
StyD4I CCNGG 7 cut(s) 886, 1294, 2037, 2366, 4330, 4424, 4690
StyI CCWWGG 4 cut(s) 1209, 3169, 3480, 3910
TaiI ACGT 7 cut(s) 216, 1656, 3704, 3724, 3892, 4184, 4581
TaqI TCGA 9 cut(s) 15, 41, 426, 509, 1177, 2107, 4098, 4490, 4530
TatI WGTACW 9 cut(s) 370, 458, 1852, 2128, 2233, 2895, 3255, 3767, 4228
TauI GCSGC 1 cut(s) 2845
TseFI GTSAC 7 cut(s) 1052, 1587, 2348, 3164, 3882, 3950, 4579
TseI GCWGC 7 cut(s) 19, 22, 98, 157, 620, 2141, 2857
Tsp45I GTSAC 7 cut(s) 1052, 1587, 2348, 3164, 3882, 3950, 4579
TspGWI ACGGA 3 cut(s) 1568, 4619, 4821
Van91I CCANNNNNTGG 3 cut(s) 359, 1697, 2601
Vha464I CTTAAG 4 cut(s) 1517, 2488, 3353, 3463
VpaK11BI GGWCC 6 cut(s) 350, 421, 976, 1262, 1552, 2713
XapI RAATTY 9 cut(s) 1563, 1833, 1963, 2679, 3148, 3475, 3574, 4073, 4294
XceI RCATGY 6 cut(s) 973, 989, 1122, 1826, 2135, 4199
XhoI CTCGAG 1 cut(s) 14
XmiI GTMKAC 2 cut(s) 1205, 3798
XmnI GAANNNNTTC 7 cut(s) 909, 1353, 1836, 2681, 3630, 3769, 4230
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 3 cut(s) 372, 3769, 4230
Zsp2I ATGCAT 1 cut(s) 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.