MD10G1339300.v1.1

Sister chromatid cohesion protein PDS5 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009376
Physical Location & Seq
Forward (+)
41501149 .. 41513769
12621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1339300.v1.1.491

Sequence Viewer

Length: 5040 bp
ATGACAGCGCCTGCCCTCCCCCACGAAAAGATGGCCCAAAAGCTCGAGCAGCAGCTCAAGGAGGTGGGATCGAAGCTCGAGACCCATCATTCTTCCAAAGACGCTCTCGTCAAGCTCTTAAAGCAAGCTGCATCATGTCTTTCGGACTTGGATCAATCACCTCCAGCGTCTATGTTGGAGTCAATGCAGCCTTTACTTAATGCAATTGTCAAGCCAGAGCTGTTGAAGCATCAAGATAGAGACGTCAAGCTTCTAGTTGCAACCTGTATTTGTGAGATAACTCGAATTACTGCACCTGAAGCTCCTTATAGTGATGAAGTGCTAAAGGACATATTTCACTTGATCGTGGGAACTTTTAGTGGATTGAAAGATACGAGTGGTCCATCATTTGGTAGAAGAGTACTCATTTTGGAGACTCTTGCCAAATATAGGTCATGTGTTGTAATGTTAGATCTTGAATGTGATGATCTGGTGAATGAAATGTTCAGTACATTTTTTGCGGTTGCCAGAGATGATCACCAAGAAACTGTTTTCTCATCGATGCAAACAATTATGATTGTGCTCTTAGAAGAGAGCGAGGAATTGCGAGATGATCTTTTGTTTATTGTATTGTCTATATTAGGTCGTAATAGAAGTGATATCACTGTGGCTGCTAGGAGGCTTGCCATGAATGTTATAGAACAGTGCGCAGGAAAGCTTGAATCTGGCATTAAACAGTTTCTTATATCATCAATGTCAGGAGACAACAAGTCTGAGAATCATCAGATTGATTACCATGAAGTCATCTATGATGTATATCGATGTGCTCCCCAAATCTTATCAGGAATTGTCCCATACCTAACAGGAGAGTTACTGACTGATCAGCTAGAAACTCGTTTAAAAGCGGTTAGTTTAGTTGGAGACCTATTTTCTCTGCCAGGCTCTACCATCTCCAAAACCTTTCAGCCAATCTTTTCGGAGTTCTTGAAGAGGTTGACCGATAGAGTTGTGGAGGTTCGCATGTTGGTCCTCCAACATGTCAAGAGCTGTATGCTGTCAAATCCTTTCAGAGCTGAAGCTCCCAAAATAATTTCTGCCCTCTGTGACCGGCTATTGGACTTTGAAGAAAAAGTACGGAAGCAAGTTGTTGCTGTAGTTTATGATGTGGCATGTCATGCCCTGAATTCAATTCCACTAGAAACTATAAAATTGGTTGCAGAGCGCCTTCGAGATAAATCTCTACTTGTTAAAACGTATACCATGGAGAGGCTAGCTGAGATATTCAGGGTTTATTGCGCAAAGTGCTCTGATGATCCACTCCTATCCAGTGATTTTGATTGGATTCCTGGGAAGATTCTGAGATGTTTTTATGACAAAGATTTCAGATCGGATACAATTGAAAATGTTCTGTGTGAGTCCCTGTTCCCTCCAAATTTTTCCCTCAAAGATAAAGTTAAACATTGGGTCAGAGTCTTCTCAGGATTTGATAAAGTTGAGGTGAAGGCTCTTGAGAAGATTCTAGAGCAGAAACAAAGGTTACAACAAGAGATGCAGAAGTATCTGGCACTTAAGCAGATGCATCAGGATGGTGATGCTCCTGAGGTCCAAAAAAAAATTCTCTACTGTTTTCGTGTAATGTCACGGTTGTTTGCTGACCCTGTTAAGGCTGAAGAGGGTTTTCAATTTCTTGATCAACTGAAAGACGTTAACATCTGGAAGATTTTAACAAATCTTATTGATCCAAACACTGGCTCCCAGCAAGCTTGCACTTTACGGGATGATTTGCTTAGGATTCTGGGTGAAAAACACCAACTGTATGAATTTCTTAGCACTCTCTCAGTGAAGTGTTCTTATCTACTTTTCAACAAAGAGCATGTGAAGGAAATTCTTCTGGAGATTCTTGTACACAAATCTACTGCAGATATGAAATATGTGCAATCTTGCATGAATATTTTGGCGATTCTTGCACGCTTTAGTCCATTATTACTCAGTGGGACAGGAGAAGAACTAGCAAATTTGCTTAACGACGATGATGAAACAATAAAAGAAGGGGTTCTGAATATTTTAGCTAGGGCTGGTGGTATCATCCGGGAGCATCTTGCAGTGTCATCAAGTTCCATAGACCTTGTATTGGAAAGGTTGTGCTTGGAGGGCAGTCGAAGACAGGCAAAGTATGCTGTACATGCCCTTGCAGCGATAACAAAGGACGATGGACTTAAATCACTCTCTGTTTTATACAAGAGGCTTGTGGATATGCTGGAGGAGAAGACACATTTGCCTACTGTACTACAATCTTTGGGGTGTATAGCTCAGACTGCAATGCCAGTTTTTGAAACTAGAGAGAAGGAAATTGAAGAGTTTATAGTTGAAAAGATCCTAAAGTGCAATAATAAATCAGGCGACAATAAAAATGCATCTTGGGATGACAAAAGTGAGCTTTGTTTGCTAAAGATATACGGGATAAAGACATTAGTGAAAAGTTATTTGCCTGTCAAAGATGCTCATGTTCGCTCTGGCATTGATGGTCTGCTGGAAATCTTAAGAAACACACTTTCTTGTGGAGAAATATCAAAAGACATAGAAACAAGTTCAGTTGACAAGGCGAATTTGAGGCTTGCTTCTGCAAAGGCAGTTCTTCGTCTGTCCAAGCACTGGAATCATAAGATACCTGTTGATATCTTCTACTTAACCTTGAAGACTTCGGAGATAAGTTTCCCTCAAGCTAAGAAATTTTTCCTGGACAAAGTTCATCAATACATAAAGGACCGGCTTTTAGATGGAAAATATGCTTGTGCATTCTTTTTTAACACATCTGGATCAAAGTCAGCAGAGTTTCAAGAGGAGAAACAGAATCTCGCAGACATTATTCAAATGTATCACCAAACAAAGACACGGCATCTCTCCGTGCAGCAATCTGATGCTAATTCATTGACTGCTTATCCGGAGTACATCCTCCCATACTTAGTTCATGCCCTTGCACATCATTCTTGTCCTAATATTGATGAATGCAAGGATGTGAAAGCATTTGAGCCGATATACCGGCAATTGCACTTATTTCTGTCTATGTTGGTGCATAGAGATGATGATGTCAAGTCAGAATCTAGTAGCAACGTTGAAAAGGAGGACATTTCTGCTATAATCTCTATCTTTCAAAGTATCAAGTGCTCTGAAGATATATATGATGCAACAAAGTCAAAGAATTCACATGGTATTTGTGACCTTGGGCTGTCAGTTACCAAACTCTTATTCCCAAAGGAGAATGATTTACAAGGATTGCCAGCATCAATTCCTCTGCCTTCAATGTTGTACAAACCATATGAGAAGAAAGAAGGAGATGACTCTGTGGCCAGCGAAGGGCAAACATGGTTGGCAGATGACAGTGTGCTGACTCACTTTGAATCTCTTAAGTTAGAGTCTAGTGAAACGGGACTTTCAGAGATTGCTGAAGATGAGCTTCTGAAAGATGGTGAAAGAGATGGAGGTGAAGTGCCTTTAGGAAAAATTATAAAGCGCTTAAGATCTCAAAATTCAAAGGCTAAAAGGGCGAAAAAGAACAAAGCATCATCAACGGATGCAGAGAATGCTGAAAATAGTGTTGATATATTGAAAATGGTCAGGGACATAAATTTGGATAATTTGGAAAAACCTTCTAAGTTTGAGTCAAGCAATGGTCATGAAAATCTTCCAAGCAAGAAATCAAGAATGGATCTAAAGCACGAAAAGGAAAACAAAAGAAAATGTACTGATGCAACATCTGTCCCCGTTCCTAAACGTAGGAGGTCGTCATCCGCTCACAGTGCTTTCAGATCACCTAGAAGCACTTCCAAGTCTCCTCTAAGTGCTTCACTAGACGATGTAGACAATAGAAAACTGTTTCAAGGCACCGAGTCAGATTTGTTGGTTTCATGCTTCCGGAAGAATGCTACCTCTTCGTCACAACGTAAAGGCAGAGCCTCTGACCGTGGTCATAATGATGAGGCAAATGAAGTTGGAGAAGCCAGTGACCGTGAGGAGCCTAATGTACTGAAGGCTGACAAGGATGACCCAAATAGTGATTTCAACTCTCCTGCAGGATCCATCAAGAAGCGGAAAAGAAAGAGTATTGCCGGTTTGGCAAAGTGCAAATTCAAGGAAAGTGGAAAAGATGTTGAGGACTTGATTGGTTGCAGAATAAAAGTTTGGTGGCCAATGGATAAGCGATTTTATGAAGGCACAGTGAAGTCATTTGATACGTTAAAGAGGAAGCATGTGATACTATATGAAGATGGAGATGTAGAAGTACTTCGGTTAGAAAAGGAACGGTGGGAGCTCATTGACAAAGGTCGCAAGCCCACAAAGAAGTTAAATTCGTCAAAGAAATCCCCCTCAAAAGAAGTGTCCCCTGGTCAAAAGACTAAGCGTGCAGGTGGTTCGCGTGAGAACAAAAAACCAATTAAAATAGTTAAAAGGAAAAGAACACCAAAGAAAAACTCGGGCGGGAAGAAGGGCGTATCAAAAAGAAATCATTGGGGAAGTAGGGACAAAGATAGTTCTGAAGTTTCAAATGATGAGCCCACCCTTACATCTAAAGTTGATGAAATGGACTCAGGTAGTTCAGAAGAGAATGCTGAAAAAGTGGATGAGAACGTGACAGATGAGGGGGAATCTGACGTGGAAGTGAAATCTGTTTCTGAAGGAAAACAGTTAAAAGATGCAGAAGAAAGCCCACATCATACCGAGGAATCTGCTGAAGAGAATCCTGGTTCCGAAGGGAGCCCTGCTGAAGACATGGACACTATCCCACAAGATGCAGAAAATGGAAATGAGGAGATGCAACACTCAGAAGAGAAAGAAGCAGATGAACTAAGTGGAGGGTCAAGAGAGGTTAACGAGGAAGATCCGTCAGATTCTGAAGGGATTCAAGAAAAAGATGATATTTCTGGCAGTCCCTTGAAACAGGAGAAATCCCATGTAGAGGCTTCAAGTCTTTCTGATGCCGGGGATGACGAACTTTCTGATGATGAGCCTCTTAGCAAGTGGACGCAACGAGTAGTGAAAAAAGGTTCGAAGCCAGTGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1680

Amino Acids

188.83

Weight (kDa)

5.93

Isoelectric Point (pI)

48.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 34 - 1097 8e-261 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3485
AarI CACCTGC 1 cut(s) 4373
AasI GACNNNNNNGTC 1 cut(s) 107
AatII GACGTC 1 cut(s) 246
Acc16I TGCGCA 2 cut(s) 688, 1276
Acc36I ACCTGC 1 cut(s) 4373
AccB1I GGYRCC 1 cut(s) 3860
AccB7I CCANNNNNTGG 3 cut(s) 389, 1727, 2631
AccBSI CCGCTC 1 cut(s) 3770
AccI GTMKAC 2 cut(s) 1235, 3837
AccII CGCG 1 cut(s) 4393
AccIII TCCGGA 2 cut(s) 2920, 3891
AciI CCGC 5 cut(s) 500, 884, 3768, 4066, 4455
AclI AACGTT 1 cut(s) 3090
AcoI YGGCCR 2 cut(s) 3324, 4163
AcyI GRCGYC 1 cut(s) 243
AfeI AGCGCT 1 cut(s) 3491
AflII CTTAAG 4 cut(s) 1547, 2518, 3383, 3493
AflIII ACRYGT 1 cut(s) 1015
AhdI GACNNNNNGTC 1 cut(s) 748
AjiI CACGTC 1 cut(s) 4630
AjnI CCWGG 5 cut(s) 916, 1324, 2715, 4360, 4717
AjuI GAANNNNNNNTTGG 6 cut(s) 1578, 1610, 3209, 3241, 4141, 4173
Alw21I GWGCWC 5 cut(s) 564, 808, 1286, 3146, 4290
Alw26I GTCTC 6 cut(s) 74, 234, 407, 735, 894, 3813
AlwNI CAGNNNCTG 2 cut(s) 11, 4868
Ama87I CYCGRG 3 cut(s) 44, 77, 4450
Aor13HI TCCGGA 2 cut(s) 2920, 3891
Aor51HI AGCGCT 1 cut(s) 3491
AoxI GGCC 3 cut(s) 33, 3324, 4163
ApeKI GCWGC 7 cut(s) 49, 52, 128, 187, 650, 2171, 2887
ArsI GACNNNNNNTTYG 2 cut(s) 4340, 4372
Asp700I GAANNNNTTC 8 cut(s) 1383, 1866, 2031, 2711, 3660, 3799, 4260, 4875
AspLEI GCGC 5 cut(s) 10, 689, 1203, 1277, 3492
AspS9I GGNCC 5 cut(s) 34, 380, 1006, 1582, 2743
AsuC2I CCSGG 2 cut(s) 2069, 4957
AsuII TTCGAA 1 cut(s) 5024
AsuNHI GCTAGC 1 cut(s) 1249
AvaI CYCGRG 3 cut(s) 44, 77, 4450
AvaII GGWCC 4 cut(s) 380, 1006, 1582, 2743
AxyI CCTNAGG 1 cut(s) 1578
BalI TGGCCA 2 cut(s) 3326, 4165
BamHI GGATCC 1 cut(s) 4052
BanI GGYRCC 1 cut(s) 3860
BanII GRGCYC 3 cut(s) 4290, 4533, 4736
BarI GAAGNNNNNNTAC 4 cut(s) 1095, 1127, 4245, 4277
BbsI GAAGAC 5 cut(s) 1444, 2146, 2252, 2681, 4749
Bbv12I GWGCWC 5 cut(s) 564, 808, 1286, 3146, 4290
BbvI GCAGC 7 cut(s) 61, 64, 115, 199, 637, 2183, 2899
BceAI ACGGC 1 cut(s) 2888
BcgI CGANNNNNNTGC 5 cut(s) 4371, 4405, 4685, 4719, 5014
BciT130I CCWGG 5 cut(s) 918, 1326, 2717, 4362, 4719
BciVI GTATCC 1 cut(s) 1363
BclI TGATCA 3 cut(s) 514, 859, 1669
BcnI CCSGG 2 cut(s) 2069, 4957
BcoDI GTCTC 6 cut(s) 74, 234, 407, 735, 894, 3813
BfmI CTRYAG 3 cut(s) 1131, 1896, 4047
BfoI RGCGCY 3 cut(s) 11, 1204, 3493
BfrI CTTAAG 4 cut(s) 1547, 2518, 3383, 3493
BfuAI ACCTGC 1 cut(s) 4373
BfuI GTATCC 1 cut(s) 1363
BglI GCCNNNNNGGC 1 cut(s) 4091
BglII AGATCT 2 cut(s) 451, 3497
BisI GCNGC 7 cut(s) 50, 53, 129, 188, 651, 2172, 2888
BlsI GCNGC 7 cut(s) 51, 54, 130, 189, 652, 2173, 2889
BmcAI AGTACT 2 cut(s) 402, 4260
Bme1390I CCNGG 7 cut(s) 918, 1326, 2069, 2717, 4362, 4719, 4957
Bme18I GGWCC 4 cut(s) 380, 1006, 1582, 2743
BmeRI GACNNNNNGTC 1 cut(s) 748
BmeT110I CYCGRG 3 cut(s) 44, 77, 4450
BmgBI CACGTC 1 cut(s) 4630
BmgT120I GGNCC 5 cut(s) 34, 380, 1006, 1582, 2743
BmiI GGNNCC 6 cut(s) 1732, 3862, 3993, 4054, 4723, 4733
BmrFI CCNGG 7 cut(s) 918, 1326, 2069, 2717, 4362, 4719, 4957
BmtI GCTAGC 1 cut(s) 1253
BoxI GACNNNNGTC 2 cut(s) 3942, 4299
BpiI GAAGAC 5 cut(s) 1444, 2146, 2252, 2681, 4749
BpmI CTGGAG 3 cut(s) 147, 1892, 2258
Bpu10I CCTNAGC 1 cut(s) 1766
Bpu14I TTCGAA 1 cut(s) 5024
BpuEI CTTGAG 3 cut(s) 41, 1508, 2682
BpuMI CCSGG 2 cut(s) 2069, 4957
Bsa29I ATCGAT 2 cut(s) 539, 799
BsaBI GATNNNNATC 1 cut(s) 795
BsaHI GRCGYC 1 cut(s) 243
BsaI GGTCTC 2 cut(s) 74, 894
BsaJI CCNNGG 7 cut(s) 1239, 1325, 3199, 3940, 4360, 4695, 4956
BsaWI WCCGGW 2 cut(s) 2920, 3891
BsaXI ACNNNNNCTCC 4 cut(s) 1715, 1745, 3450, 3480
Bse118I RCCGGY 4 cut(s) 1086, 2745, 3018, 4085
Bse1I ACTGG 6 cut(s) 1305, 1732, 2303, 2636, 3978, 5030
Bse21I CCTNAGG 1 cut(s) 1578
Bse3DI GCAATG 2 cut(s) 2304, 3652
Bse8I GATNNNNATC 1 cut(s) 795
BseAI TCCGGA 2 cut(s) 2920, 3891
BseBI CCWGG 5 cut(s) 918, 1326, 2717, 4362, 4719
BseCI ATCGAT 2 cut(s) 539, 799
BseDI CCNNGG 7 cut(s) 1239, 1325, 3199, 3940, 4360, 4695, 4956
BseJI GATNNNNATC 1 cut(s) 795
BseMI GCAATG 2 cut(s) 2304, 3652
BseNI ACTGG 6 cut(s) 1305, 1732, 2303, 2636, 3978, 5030
BseRI GAGGAG 5 cut(s) 2255, 2834, 3801, 4004, 4799
BseXI GCAGC 7 cut(s) 61, 64, 115, 199, 637, 2183, 2899
BseYI CCCAGC 1 cut(s) 1734
BsgI GTGCAG 3 cut(s) 276, 2906, 4401
Bsh1236I CGCG 1 cut(s) 4393
BshFI GGCC 3 cut(s) 35, 3326, 4165
BshNI GGYRCC 1 cut(s) 3860
BshVI ATCGAT 2 cut(s) 539, 799
BsiHKAI GWGCWC 5 cut(s) 564, 808, 1286, 3146, 4290
BsiHKCI CYCGRG 3 cut(s) 44, 77, 4450
BsiSI CCGG 8 cut(s) 1087, 2068, 2746, 2921, 3019, 3892, 4086, 4956
BslFI GGGAC 9 cut(s) 815, 1381, 1987, 3420, 3611, 3722, 4342, 4511, 4890
BsmAI GTCTC 6 cut(s) 74, 234, 407, 735, 894, 3813
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 9 cut(s) 815, 1381, 1987, 3420, 3611, 3722, 4342, 4511, 4890
BsmI GAATGC 5 cut(s) 2774, 2990, 3565, 3904, 4588
BsnI GGCC 3 cut(s) 35, 3326, 4165
Bso31I GGTCTC 2 cut(s) 74, 894
BsoBI CYCGRG 3 cut(s) 44, 77, 4450
Bsp119I TTCGAA 1 cut(s) 5024
Bsp1286I GDGCHC 7 cut(s) 564, 808, 1286, 3146, 4290, 4533, 4736
Bsp13I TCCGGA 2 cut(s) 2920, 3891
Bsp1407I TGTACA 3 cut(s) 1882, 2158, 3285
Bsp19I CCATGG 1 cut(s) 1239
BspACI CCGC 5 cut(s) 500, 884, 3768, 4066, 4455
BspANI GGCC 3 cut(s) 35, 3326, 4165
BspDI ATCGAT 2 cut(s) 539, 799
BspEI TCCGGA 2 cut(s) 2920, 3891
BspFNI CGCG 1 cut(s) 4393
BspHI TCATGA 1 cut(s) 3652
BspLI GGNNCC 6 cut(s) 1732, 3862, 3993, 4054, 4723, 4733
BspMAI CTGCAG 2 cut(s) 1900, 4051
BspMI ACCTGC 1 cut(s) 4373
BspOI GCTAGC 1 cut(s) 1253
BspT104I TTCGAA 1 cut(s) 5024
BspT107I GGYRCC 1 cut(s) 3860
BspTI CTTAAG 4 cut(s) 1547, 2518, 3383, 3493
BspTNI GGTCTC 2 cut(s) 74, 894
BsrBI CCGCTC 1 cut(s) 3770
BsrDI GCAATG 2 cut(s) 2304, 3652
BsrFI RCCGGY 4 cut(s) 1086, 2745, 3018, 4085
BsrGI TGTACA 3 cut(s) 1882, 2158, 3285
BsrI ACTGG 6 cut(s) 1305, 1732, 2303, 2636, 3978, 5030
BssAI RCCGGY 4 cut(s) 1086, 2745, 3018, 4085
BssECI CCNNGG 7 cut(s) 1239, 1325, 3199, 3940, 4360, 4695, 4956
BssNAI GTATAC 1 cut(s) 1236
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 2 cut(s) 1239, 3199
Bst1107I GTATAC 1 cut(s) 1236
Bst2UI CCWGG 5 cut(s) 918, 1326, 2717, 4362, 4719
Bst6I CTCTTC 9 cut(s) 391, 564, 962, 1644, 2328, 3913, 4572, 4704, 4797
BstACI GRCGYC 1 cut(s) 243
BstAFI CTTAAG 4 cut(s) 1547, 2518, 3383, 3493
BstAPI GCANNNNNTGC 3 cut(s) 1154, 2153, 3560
BstAUI TGTACA 3 cut(s) 1882, 2158, 3285
BstBI TTCGAA 1 cut(s) 5024
BstDSI CCRYGG 2 cut(s) 1239, 3940
BstFNI CGCG 1 cut(s) 4393
BstH2I RGCGCY 3 cut(s) 11, 1204, 3493
BstHHI GCGC 5 cut(s) 10, 689, 1203, 1277, 3492
BstMAI GTCTC 6 cut(s) 74, 234, 407, 735, 894, 3813
BstNI CCWGG 5 cut(s) 918, 1326, 2717, 4362, 4719
BstNSI RCATGY 6 cut(s) 1003, 1019, 1152, 1856, 2165, 4229
BstPAI GACNNNNGTC 2 cut(s) 3942, 4299
BstSCI CCNGG 7 cut(s) 916, 1324, 2067, 2715, 4360, 4717, 4955
BstSFI CTRYAG 3 cut(s) 1131, 1896, 4047
BstUI CGCG 1 cut(s) 4393
BstV1I GCAGC 7 cut(s) 61, 64, 115, 199, 637, 2183, 2899
BstV2I GAAGAC 5 cut(s) 1444, 2146, 2252, 2681, 4749
BstX2I RGATCY 6 cut(s) 451, 2352, 3497, 3685, 4052, 4855
BstYI RGATCY 6 cut(s) 451, 2352, 3497, 3685, 4052, 4855
BstZ17I GTATAC 1 cut(s) 1236
Bsu15I ATCGAT 2 cut(s) 539, 799
Bsu36I CCTNAGG 1 cut(s) 1578
BsuI GTATCC 1 cut(s) 1363
BsuRI GGCC 3 cut(s) 35, 3326, 4165
BsuTUI ATCGAT 2 cut(s) 539, 799
BtgI CCRYGG 2 cut(s) 1239, 3940
BtrI CACGTC 1 cut(s) 4630
BtsI GCAGTG 1 cut(s) 2088
BveI ACCTGC 1 cut(s) 4373
CaiI CAGNNNCTG 2 cut(s) 11, 4868
CciI TCATGA 1 cut(s) 3652
CfoI GCGC 5 cut(s) 10, 689, 1203, 1277, 3492
Cfr10I RCCGGY 4 cut(s) 1086, 2745, 3018, 4085
Cfr13I GGNCC 5 cut(s) 34, 380, 1006, 1582, 2743
ClaI ATCGAT 2 cut(s) 539, 799
CseI GACGC 3 cut(s) 110, 156, 5008
CspCI CAANNNNNGTGG 2 cut(s) 4096, 4131
DraI TTTAAA 1 cut(s) 879
DrdI GACNNNNNNGTC 1 cut(s) 107
DriI GACNNNNNGTC 1 cut(s) 748
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 2 cut(s) 3324, 4163
Eam1104I CTCTTC 9 cut(s) 391, 564, 962, 1644, 2328, 3913, 4572, 4704, 4797
Eam1105I GACNNNNNGTC 1 cut(s) 748
EarI CTCTTC 9 cut(s) 391, 564, 962, 1644, 2328, 3913, 4572, 4704, 4797
Ecl136II GAGCTC 1 cut(s) 4288
Eco130I CCWWGG 2 cut(s) 1239, 3199
Eco24I GRGCYC 3 cut(s) 4290, 4533, 4736
Eco31I GGTCTC 2 cut(s) 74, 894
Eco32I GATATC 2 cut(s) 640, 2656
Eco47I GGWCC 4 cut(s) 380, 1006, 1582, 2743
Eco47III AGCGCT 1 cut(s) 3491
Eco53kI GAGCTC 1 cut(s) 4288
Eco81I CCTNAGG 1 cut(s) 1578
Eco88I CYCGRG 3 cut(s) 44, 77, 4450
EcoICRI GAGCTC 1 cut(s) 4288
EcoRI GAATTC 2 cut(s) 1162, 3178
EcoRII CCWGG 5 cut(s) 916, 1324, 2715, 4360, 4717
EcoRV GATATC 2 cut(s) 640, 2656
EcoT14I CCWWGG 2 cut(s) 1239, 3199
EcoT22I ATGCAT 2 cut(s) 1560, 2395
EcoT38I GRGCYC 3 cut(s) 4290, 4533, 4736
ErhI CCWWGG 2 cut(s) 1239, 3199
Esp3I CGTCTC 1 cut(s) 234
FalI AAGNNNNNCTT 2 cut(s) 3417, 3449
FaqI GGGAC 9 cut(s) 815, 1381, 1987, 3420, 3611, 3722, 4342, 4511, 4890
FauI CCCGC 1 cut(s) 4448
FauNDI CATATG 1 cut(s) 3295
FbaI TGATCA 3 cut(s) 514, 859, 1669
FblI GTMKAC 2 cut(s) 1235, 3837
Fnu4HI GCNGC 7 cut(s) 50, 53, 129, 188, 651, 2172, 2888
FriOI GRGCYC 3 cut(s) 4290, 4533, 4736
Fsp4HI GCNGC 7 cut(s) 50, 53, 129, 188, 651, 2172, 2888
FspI TGCGCA 2 cut(s) 688, 1276
GlaI GCGC 5 cut(s) 9, 688, 1202, 1276, 3491
GluI GCNGC 7 cut(s) 50, 53, 129, 188, 651, 2172, 2888
GsaI CCCAGC 1 cut(s) 1738
GsuI CTGGAG 3 cut(s) 147, 1892, 2258
HaeII RGCGCY 3 cut(s) 11, 1204, 3493
HaeIII GGCC 3 cut(s) 35, 3326, 4165
HapII CCGG 8 cut(s) 1087, 2068, 2746, 2921, 3019, 3892, 4086, 4956
HgaI GACGC 3 cut(s) 110, 156, 5008
HhaI GCGC 5 cut(s) 10, 689, 1203, 1277, 3492
Hin1I GRCGYC 1 cut(s) 243
Hin6I GCGC 5 cut(s) 8, 687, 1201, 1275, 3490
HinP1I GCGC 5 cut(s) 8, 687, 1201, 1275, 3490
HincII GTYRAC 4 cut(s) 975, 1687, 2575, 4846
HindII GTYRAC 4 cut(s) 975, 1687, 2575, 4846
HindIII AAGCTT 3 cut(s) 248, 695, 1740
HpaI GTTAAC 2 cut(s) 1687, 4846
HpaII CCGG 8 cut(s) 1087, 2068, 2746, 2921, 3019, 3892, 4086, 4956
Hpy166II GTNNAC 8 cut(s) 975, 1236, 1687, 1885, 2575, 3838, 4846, 4998
Hpy8I GTNNAC 8 cut(s) 975, 1236, 1687, 1885, 2575, 3838, 4846, 4998
Hpy99I CGWCG 1 cut(s) 2009
HpyCH4IV ACGT 9 cut(s) 243, 1232, 1683, 3090, 3751, 3919, 4211, 4605, 4629
HpySE526I ACGT 9 cut(s) 243, 1232, 1683, 3090, 3751, 3919, 4211, 4605, 4629
Hsp92I GRCGYC 1 cut(s) 243
HspAI GCGC 5 cut(s) 8, 687, 1201, 1275, 3490
Kpn2I TCCGGA 2 cut(s) 2920, 3891
Ksp22I TGATCA 3 cut(s) 514, 859, 1669
KspAI GTTAAC 2 cut(s) 1687, 4846
LmnI GCTCC 9 cut(s) 307, 811, 1063, 1579, 1736, 2071, 3991, 4285, 4731
Lsp1109I GCAGC 7 cut(s) 61, 64, 115, 199, 637, 2183, 2899
MaeII ACGT 9 cut(s) 243, 1232, 1683, 3090, 3751, 3919, 4211, 4605, 4629
MaeIII GTNAC 9 cut(s) 849, 1082, 1515, 1617, 3194, 3211, 3912, 3980, 4606
MbiI CCGCTC 1 cut(s) 3770
MfeI CAATTG 3 cut(s) 204, 1374, 3023
MflI RGATCY 6 cut(s) 451, 2352, 3497, 3685, 4052, 4855
MhlI GDGCHC 7 cut(s) 564, 808, 1286, 3146, 4290, 4533, 4736
MlsI TGGCCA 2 cut(s) 3326, 4165
MluNI TGGCCA 2 cut(s) 3326, 4165
MmeI TCCRAC 4 cut(s) 156, 877, 1036, 3949
Mox20I TGGCCA 2 cut(s) 3326, 4165
Mph1103I ATGCAT 2 cut(s) 1560, 2395
MroI TCCGGA 2 cut(s) 2920, 3891
MroXI GAANNNNTTC 8 cut(s) 1383, 1866, 2031, 2711, 3660, 3799, 4260, 4875
MscI TGGCCA 2 cut(s) 3326, 4165
MslI CAYNNNNRTG 3 cut(s) 1563, 3057, 3951
Msp20I TGGCCA 2 cut(s) 3326, 4165
MspCI CTTAAG 4 cut(s) 1547, 2518, 3383, 3493
MspI CCGG 8 cut(s) 1087, 2068, 2746, 2921, 3019, 3892, 4086, 4956
MspR9I CCNGG 7 cut(s) 918, 1326, 2069, 2717, 4362, 4719, 4957
MunI CAATTG 3 cut(s) 204, 1374, 3023
Mva1269I GAATGC 5 cut(s) 2774, 2990, 3565, 3904, 4588
MvaI CCWGG 5 cut(s) 918, 1326, 2717, 4362, 4719
MvnI CGCG 1 cut(s) 4393
NciI CCSGG 2 cut(s) 2069, 4957
NcoI CCATGG 1 cut(s) 1239
NdeI CATATG 1 cut(s) 3295
NheI GCTAGC 1 cut(s) 1249
NlaIV GGNNCC 6 cut(s) 1732, 3862, 3993, 4054, 4723, 4733
NmuCI GTSAC 6 cut(s) 1082, 1617, 3194, 3912, 3980, 4606
NsbI TGCGCA 2 cut(s) 688, 1276
NsiI ATGCAT 2 cut(s) 1560, 2395
NspI RCATGY 6 cut(s) 1003, 1019, 1152, 1856, 2165, 4229
NspV TTCGAA 1 cut(s) 5024
PaeR7I CTCGAG 2 cut(s) 44, 77
PagI TCATGA 1 cut(s) 3652
PaqCI CACCTGC 1 cut(s) 4373
PciI ACATGT 1 cut(s) 1015
PctI GAATGC 5 cut(s) 2774, 2990, 3565, 3904, 4588
PdmI GAANNNNTTC 8 cut(s) 1383, 1866, 2031, 2711, 3660, 3799, 4260, 4875
PflMI CCANNNNNTGG 3 cut(s) 389, 1727, 2631
PfoI TCCNGGA 2 cut(s) 2067, 2715
PkrI GCNGC 7 cut(s) 51, 54, 130, 189, 652, 2173, 2889
PscI ACATGT 1 cut(s) 1015
PshAI GACNNNNGTC 2 cut(s) 3942, 4299
PsiI TTATAA 1 cut(s) 3485
Psp124BI GAGCTC 1 cut(s) 4290
Psp1406I AACGTT 1 cut(s) 3090
Psp6I CCWGG 5 cut(s) 916, 1324, 2715, 4360, 4717
PspFI CCCAGC 1 cut(s) 1734
PspGI CCWGG 5 cut(s) 916, 1324, 2715, 4360, 4717
PspN4I GGNNCC 6 cut(s) 1732, 3862, 3993, 4054, 4723, 4733
PspPI GGNCC 5 cut(s) 34, 380, 1006, 1582, 2743
PspXI VCTCGAGB 1 cut(s) 44
PstI CTGCAG 2 cut(s) 1900, 4051
PstNI CAGNNNCTG 2 cut(s) 11, 4868
PsuI RGATCY 6 cut(s) 451, 2352, 3497, 3685, 4052, 4855
RseI CAYNNNNRTG 3 cut(s) 1563, 3057, 3951
SacI GAGCTC 1 cut(s) 4290
SatI GCNGC 7 cut(s) 50, 53, 129, 188, 651, 2172, 2888
Sau96I GGNCC 5 cut(s) 34, 380, 1006, 1582, 2743
SbfI CCTGCAGG 1 cut(s) 4051
ScaI AGTACT 2 cut(s) 402, 4260
ScrFI CCNGG 7 cut(s) 918, 1326, 2069, 2717, 4362, 4719, 4957
SdaI CCTGCAGG 1 cut(s) 4051
SduI GDGCHC 7 cut(s) 564, 808, 1286, 3146, 4290, 4533, 4736
SfcI CTRYAG 3 cut(s) 1131, 1896, 4047
Sfr274I CTCGAG 2 cut(s) 44, 77
SfuI TTCGAA 1 cut(s) 5024
SinI GGWCC 4 cut(s) 380, 1006, 1582, 2743
SlaI CTCGAG 2 cut(s) 44, 77
SmiMI CAYNNNNRTG 3 cut(s) 1563, 3057, 3951
SmlI CTYRAG 9 cut(s) 44, 56, 77, 1487, 1547, 2518, 2697, 3383, 3493
SmoI CTYRAG 9 cut(s) 44, 56, 77, 1487, 1547, 2518, 2697, 3383, 3493
Sse8387I CCTGCAGG 1 cut(s) 4051
SsiI CCGC 5 cut(s) 500, 884, 3768, 4066, 4455
SspI AATATT 3 cut(s) 1930, 2041, 2977
SstI GAGCTC 1 cut(s) 4290
StyD4I CCNGG 7 cut(s) 916, 1324, 2067, 2715, 4360, 4717, 4955
StyI CCWWGG 2 cut(s) 1239, 3199
TaiI ACGT 9 cut(s) 246, 1235, 1686, 3093, 3754, 3922, 4214, 4608, 4632
TaqI TCGA 9 cut(s) 45, 71, 78, 283, 539, 799, 1207, 2137, 5024
TaqII GACCGA 1 cut(s) 992
TseFI GTSAC 6 cut(s) 1082, 1617, 3194, 3912, 3980, 4606
TseI GCWGC 7 cut(s) 49, 52, 128, 187, 650, 2171, 2887
Tsp45I GTSAC 6 cut(s) 1082, 1617, 3194, 3912, 3980, 4606
TspGWI ACGGA 4 cut(s) 1129, 2872, 3563, 4848
Van91I CCANNNNNTGG 3 cut(s) 389, 1727, 2631
Vha464I CTTAAG 4 cut(s) 1547, 2518, 3383, 3493
VpaK11BI GGWCC 4 cut(s) 380, 1006, 1582, 2743
XbaI TCTAGA 1 cut(s) 1498
XceI RCATGY 6 cut(s) 1003, 1019, 1152, 1856, 2165, 4229
XhoI CTCGAG 2 cut(s) 44, 77
XmiI GTMKAC 2 cut(s) 1235, 3837
XmnI GAANNNNTTC 8 cut(s) 1383, 1866, 2031, 2711, 3660, 3799, 4260, 4875
ZraI GACGTC 1 cut(s) 244
ZrmI AGTACT 2 cut(s) 402, 4260
Zsp2I ATGCAT 2 cut(s) 1560, 2395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.