RLG00000030871

Sister chromatid cohesion protein PDS5 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009376
Physical Location & Seq
Reverse (-)
328742 .. 340302
11561 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030871

Sequence Viewer

Length: 5013 bp
ATGGCCCAGAAGGTTGAGCAGCAGCTCAAGGAGGTGGGATCCAAGCTCGAGACCCCTCATTCGTCCAAAGACGCTCTCGTCAAGCTCCTCAAGCAAGCTCAGTTATGTCTTTCTGAGTTGGATCAGTCACCTCCAGCATCTACCTTGGAGTCAATGCAGCCTTTTCTTAATGCAATAGTCAAGCCAGAACTGTTGAAGCATCAAGATAGAGATGTCAAGCTTCTAGTAGCAACATGTATTTGTGAGATAACCCGGATAACTGCACCAGAAGCTCCTTATAGTGATGATGTGCTAAAGGATGTTTTTCATCTGATTGTGGGCACTTTTAGTGGATTGAAAGATACAAGTGGTCCATCATTTGGTAGGAGAGTTGTCATACTGGAGACTCTTGCAAAGTATAGGTCATGTGTTGTAATGCTGGACCTCGAATGTGATGATCTGGTGACTGAAATGTTCAGTACATTTTTTGCTGTTGCCAGAGATGATCACCAAGAAACTGTACTGTCATCAATGCAAACAATCATGATTGTTCTTATAGAAGAGAGCGAGGATTTGCAAGAGGATCTTTTATTTGTCATCTTGTCTGTGCTAGGTCGTAACAGAAGTGATATCAATGTGGCTGCTAGGAGACTTGCTATGAATGTTATAGAACAGTGTGCAGGAAAGCTTGAGTCCGGTATCAAGCAGTTCCTTATATCATCAATGTCAGGCGACAACAAGTCAACCAGTCATCAAATTGATTACCATGAAGTCATCTATGATGTATATCGTTGTGCCCCCCAAATTGTGTCAGCAGTTGTCCCTTACCTAACTGGAGAGCTACTGACTGATCAGCTGGACACTCGTTTAAAAGCAGTTAACTTAGTTGGAGACTTATTTTCACTGCCGGGCTCTACCATTTCAGAACCATTTCAGCCAATTTTTTCAGAATTTTTGAAGAGGTTGACTGATAGAGTTGTCGAGGTTCGAATGTCAGTCCTTGAACATGTCAAGAGCTGTATGATGTCAAATCCTTTCAGAGCTGAAGCTCCCGAAATAATCTCTGCCCTCTGTGACCGGCTGTTGGACTTCGAAGAGAAAGTTCGGAAGCAAGTTGTTGCTGTAATTTATGATGTGGCATGTCATGACCTCAATTCCATTCCGCTAGAAACTGTACAACTTGTTGCCGAGCGCCTTCGAGACAAATCTGTACTTGTTAAAAAGTATACCATGGAGAGGCTGGCTGAGATGTATAGGGTTTATTGTGCAAAGTGCTCTGATGGCTCGACCATTTCCAGTGAATTTGAGTGGATTCCTGGGAAGATTCTGAGATGTTTTTATGACAAAGATTTCAGATCTGATACAATTGAAAATGTTCTTTGTGAGTCTCTATTCCCAACTGATTTCTCTACCAAAGATAAAGTTAAACATTGGGTAAGGGTCTTCTCAGTATTTGATAAAGTTGAGGTGAAGGCACTTGAGAAGATACTGGAGCAGAAGCAAAGGTTACTACAAGAGATGCAGAAGTATATGTCACTTAGGCAGGTGCATCAGGATGGTGATGCTCCTGAGATCCAAAAAAAGATTCAGTTTTGTTTTCGTATAATGGCACGCTCGTTTGCTGACCCTGCAAAGGCTGAGGAAAATTTTCAGTTTCTTGATCAATTGAAGGATGCTAACATCTGGAAGATTCTAACGAACCTTGTTGATCCAAACACTAGCTTCCATCAAGCCCAAACTTTACGGGATGATTTGCTTAAAATTTTGGGCGAAAAACACCGACTATATGACTTTCTTAGTACTCTCTCCGTGAAATGTTCTTATCTACTTTTCAACAAGGAGCATGTGAAGGAAATTCTTCTGGAGGTTGCCATGCACAGATCTACTGCAGATATACAATATAAGCTATCTTGTATGAATATTTTGGTGATTCTTGCACGGTTCAGTCCATTGCTACTTAGTGGAACAGAAGAGGAACTGGTAAATTTTCTCAAAGATGATGATGAAGCTATAAAAGAAGGAGTTTTGAATGTTTTAGCAAAGGCTGGTGGTACCATCCGGGAAAATCTTGCAGCGTTATCAAGTTCGATAGACCTTATATTGGAGAGGTTGTGCTTGGAGGGCAGTAGAAGACAGGCAAAATATGCTGTACATGCCTTGGCGGCTATAACAAAGGATGATGGGCTTAAATCACTTTCTGTTTTGTACAAGAGGCTTGTGGATATGCTCGAGGAGAAGACACATTTGCCTGCTGTACTACAATCTTTGGGGTGTATAGCTGAGACTGCAATGCCAGTCTTCGAAACTAGAGAAAGTGAAATTGAAAAGTTTATAATAGAGAAGATCCTAAAACACAATGATAAACCAGGTGATAATAAGAAGGCATCTTGGGATGACAAAAGTGAGCTTTGTTCCTTAAAGATATATGGAATTAAGACCTTGGTGAAAAGCTACTTGCCTGTCAAAGATGCTCAGGTTCGGCCCGGCATTGATGGTCTACTGGAAATATTAAGAAACACACTTTCTTGTGGAGAAATATCCAAAGACATAGAATCAAGTTCAATTGACAAGGCCCATTTAAGGCTTGCGTCTGCAAAGGCCGTTCTTCGTCTATCAAAGCACTGGAATCATAAGATACCTGTTGATGTCTTCCACTTAACCTTGAAGGTTTCAGAGATTAGCTTCCCTCAAGCAAGGAAACTATTCCTTAACAAAGTTCATCAATACATAAAGGACCGGCTTTTGGATGCAAAATATGCTTGTGCATTCTTTTTCAACATATTTGGATCAAAGTCGGCAGAATTTCAAGAGGAGAAACAAAATCTAGCTGACATTATTCAAATGTATCACCAAACAAAGGCTCGACATCTCTCTATTCAATCTGATGCTAATTCCTTGACTGCATATCCGGAGTACATCCTCCCTTACTTGGTTCATGCGCTTGCCCATCATTGTTGTCCTAATATTGATGAATCCAAGGATGATGTCAAAGCTTTTGAACTGATATACAGGCAATTGCACTTAATTCTGTCTATGTTGCTACATAGAGATGAAGATGTCAAGTCGGAATCAGGTAGCAACATTGAAAAGGAGGACCTTTCTGCTATAGTCTCTATCTTTCACAGTATCAAGACCTCTGAAGATATATATGATGCAGCGAAGTCAAAGAATTCACATGCTATTTGTGACCTTGGGCTGTCAATTACCAAGCGCTTAGCCCCAAAGGAAACTGATTTACAAGTGTTGACTGCATCAGTTCCTTTGCCTTCTATGCTGTATAAACCGTATGAGAAAAAGGAAGGAGATGATTCAGTGAATATTGAAGCACAAACGTGGTTGGCTGATGACAGTGTGCTGACTCACTTTGAATCACTTAAGTTAGACACTACTGAAACGGGAATTTCCATAAGTGCTGAGGATGAAGTTCTGATAGATGGTGAAAAAGATGGAAATGAAGTGCCTTTGGGAAAAATTATAAAGCACTTGAAGTCTCAGAAGATCAAGGCTAAAAAGGTGAAAAAGAACAAGGTTTCATCTGCTAATCCAGAGAAGGCTGAGAATGATGTTGATATCTTGAATATGGTCCGGGAGATAAATTTGGATAACTTGGGAGAATCAAGTAAGTTTGAATCAAGTAATGGTCATGAAAATCTGCCCGGCAAGAAATCAAGAATAGATACAACGCACCAAAAGGGTAACAAAAGAAAAGCTAGTGATGGAGCATCTGTTCCAGTACCTAAACGGAGGAGGTCATCCTCCACTCATAGTGCTATCAAATCTCCCAGAAGTACATCAAAGTCTCCTTTAAGCGCTTCACTTGATGATTCACACAATAGAAAATTGGGTGAAAGCATGGAGTCAGCTTTGTTGGTATCGTGCATCCGGAAGAATGCTACCTCATCAAAACGTAAAGGCAGAGCTTCAGATCCTGGTCTTCATGATGAGGAAAATGAAGTAGGAGAAGACAGTGATCGTGACGAGCCCAATGTGCTTGAGACTGGCAAGAACGACCCAAATAGTGAGTACCAGTCTCCCACAAGACCCATCAAGAAGCCGAAAAGAAAAAGCATGTCAGGATCTGCAAAGTCCAAATTCAAGGAAGGTGGAAAAGATATCGAAGACTTGATTGGCTGCAGAATAAAAGTGTGGTGGCCTATGGATAGGAAATTTTATGAAGGGACGGTGAAGTCTTATGATACACTAAAGAGAAAGCATGTGGTACTTTATGCGGATGGTGATGTAGAAGTGCTTCGGCTAGAAAAGGAACGGTGGGAGCTCGTTGACAATGGTCGCAAGCCCACAAAGAAGTCAAATTCTTCGAAGAAATCCCCTTCAAAAGAAGTGACACCTGGTCAAAAAAGTAAAAGTATGGGTAGTTCTAGTAAGAGCAAGAAATCCACTAAAACAGTTAAGAAGAAAAGAACACCGAGTAAAAACTTGGACGGGAAAAAGGGTGGATCAAAGAGAAAACAGTGGGGAAGTAGGGAAAGAGAGAGTTCTGATGTGTCGAATATTGAGCCTAATTTGGTCTCTAAAGTGGATGAAATGAACTCAGGTAGTTCAGGAGGTGCTGAAAGAAAGGATGTGAATGTGTCAGATGCGGTGGACTCTGACAAGGAAGTGAAGTCAGTTTCCAAAGGAAATCTGCTGGAAGAGGCAGAAAGTCCAAATCCAAATATTGAGGACTCTGATGAAGAGATGCCTGATTCTGAAGTGAGGCCTGCTGGAGATATGGACAGCATCGGACAAGATACTGAAAATACAGTTGACGAGAAGCTTCCTTCAGAAGAGAAACAAACAGATGAATTAAATAGATCTTCCTCGAGAGAGGATAATGAGGAAGAGTCGTCAGATTCTGAAGGGAGTAAAGAAAAAAATGATGTCCATGGTGACCGTACAAAACTGAAGAAACCCCATGTACCTGTGGAGCCCTCAAGCCCTTCTGATGCTGGGGATCTTGAACTTTCCGATGATGAACCCCTTAGCAATTTTACACTCCGTGTAGGGAAGAGAGCTTCAAAGAGAGTACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1671

Amino Acids

187.61

Weight (kDa)

6.15

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 24 - 1087 1e-267 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2312, 3455
AarI CACCTGC 1 cut(s) 1513
AasI GACNNNNNNGTC 2 cut(s) 77, 4165
Acc36I ACCTGC 1 cut(s) 1513
Acc65I GGTACC 1 cut(s) 2030
AccB1I GGYRCC 1 cut(s) 2030
AccB7I CCANNNNNTGG 1 cut(s) 359
AccI GTMKAC 2 cut(s) 1205, 2476
AccIII TCCGGA 2 cut(s) 2887, 3861
AciI CCGC 4 cut(s) 1142, 2141, 4208, 4580
AcuI CTGAAG 7 cut(s) 1044, 3138, 3885, 4710, 4746, 4857, 4904
AdeI CACNNNGTG 1 cut(s) 4979
AfeI AGCGCT 2 cut(s) 3191, 3790
AfiI CCNNNNNNNGG 9 cut(s) 359, 1063, 1215, 1612, 2080, 2559, 2722, 2836, 2908
AflII CTTAAG 1 cut(s) 3353
AflIII ACRYGT 2 cut(s) 233, 985
AhdI GACNNNNNGTC 2 cut(s) 718, 4329
AjnI CCWGG 4 cut(s) 1294, 2344, 3907, 4327
AjuI GAANNNNNNNTTGG 4 cut(s) 1548, 1580, 4089, 4121
AleI CACNNNNGTG 1 cut(s) 3310
AloI GAACNNNNNNTCC 2 cut(s) 3690, 3722
Alw21I GWGCWC 2 cut(s) 1256, 4257
AlwNI CAGNNNCTG 3 cut(s) 3908, 4058, 4835
Ama87I CYCGRG 3 cut(s) 47, 2207, 4801
Aor13HI TCCGGA 2 cut(s) 2887, 3861
Aor51HI AGCGCT 2 cut(s) 3191, 3790
AoxI GGCC 6 cut(s) 3, 2459, 2550, 2577, 4130, 4697
ApeKI GCWGC 7 cut(s) 19, 22, 157, 620, 2051, 3134, 4110
ArsI GACNNNNNNTTYG 4 cut(s) 43, 75, 386, 418
Asp700I GAANNNNTTC 6 cut(s) 909, 1353, 1626, 1836, 2681, 4227
Asp718I GGTACC 1 cut(s) 2030
AspLEI GCGC 4 cut(s) 1173, 2920, 3192, 3791
AspS9I GGNCC 8 cut(s) 4, 350, 421, 2460, 2551, 2713, 3073, 3562
AsuC2I CCSGG 6 cut(s) 253, 888, 2039, 2463, 3566, 3636
AsuII TTCGAA 4 cut(s) 967, 1071, 2280, 4298
AvaI CYCGRG 3 cut(s) 47, 2207, 4801
AvaII GGWCC 5 cut(s) 350, 421, 2713, 3073, 3562
BaeGI GKGCMC 2 cut(s) 323, 778
BamHI GGATCC 1 cut(s) 38
BanI GGYRCC 1 cut(s) 2030
BanII GRGCYC 4 cut(s) 893, 3963, 4257, 4911
BarI GAAGNNNNNNTAC 2 cut(s) 4212, 4244
BbsI GAAGAC 8 cut(s) 1414, 2116, 2222, 2269, 2620, 3905, 3948, 4104
Bbv12I GWGCWC 2 cut(s) 1256, 4257
BbvCI CCTCAGC 2 cut(s) 1617, 3393
BbvI GCAGC 7 cut(s) 31, 34, 169, 607, 2063, 3146, 4097
BceAI ACGGC 1 cut(s) 2564
BciT130I CCWGG 4 cut(s) 1296, 2346, 3909, 4329
BclI TGATCA 3 cut(s) 484, 829, 1639
BcnI CCSGG 6 cut(s) 253, 888, 2039, 2463, 3566, 3636
BfmI CTRYAG 3 cut(s) 1866, 3084, 4111
BfoI RGCGCY 3 cut(s) 1174, 3193, 3792
BfrI CTTAAG 1 cut(s) 3353
BfuAI ACCTGC 1 cut(s) 1513
BglI GCCNNNNNGGC 1 cut(s) 2141
BglII AGATCT 3 cut(s) 1334, 1859, 4793
BisI GCNGC 8 cut(s) 20, 23, 158, 621, 2052, 2142, 3135, 4111
BlpI GCTNAGC 1 cut(s) 3193
BlsI GCNGC 8 cut(s) 21, 24, 159, 622, 2053, 2143, 3136, 4112
BmcAI AGTACT 1 cut(s) 1780
Bme18I GGWCC 5 cut(s) 350, 421, 2713, 3073, 3562
BmeRI GACNNNNNGTC 2 cut(s) 718, 4329
BmeT110I CYCGRG 3 cut(s) 47, 2207, 4801
BmgT120I GGNCC 8 cut(s) 4, 350, 421, 2460, 2551, 2713, 3073, 3562
BmiI GGNNCC 3 cut(s) 40, 2032, 4908
BoxI GACNNNNGTC 1 cut(s) 4266
BpiI GAAGAC 8 cut(s) 1414, 2116, 2222, 2269, 2620, 3905, 3948, 4104
BpmI CTGGAG 6 cut(s) 117, 401, 834, 1490, 1862, 4725
Bpu10I CCTNAGC 4 cut(s) 1617, 2451, 3393, 4961
Bpu1102I GCTNAGC 1 cut(s) 3193
Bpu14I TTCGAA 4 cut(s) 967, 1071, 2280, 4298
BpuEI CTTGAG 7 cut(s) 11, 74, 689, 1478, 2652, 3992, 4897
BpuMI CCSGG 6 cut(s) 253, 888, 2039, 2463, 3566, 3636
BsaBI GATNNNNATC 1 cut(s) 765
BsaI GGTCTC 2 cut(s) 44, 4513
BsaJI CCNNGG 8 cut(s) 144, 1209, 1295, 2136, 2418, 2955, 3169, 4864
BsaWI WCCGGW 3 cut(s) 674, 2887, 3861
BsaXI ACNNNNNCTCC 4 cut(s) 2075, 2105, 3690, 3720
Bsc4I CCNNNNNNNGG 9 cut(s) 359, 1063, 1215, 1612, 2080, 2559, 2722, 2836, 2908
Bse118I RCCGGY 2 cut(s) 1056, 2715
Bse3DI GCAATG 2 cut(s) 1928, 2274
Bse8I GATNNNNATC 1 cut(s) 765
BseAI TCCGGA 2 cut(s) 2887, 3861
BseBI CCWGG 4 cut(s) 1296, 2346, 3909, 4329
BseDI CCNNGG 8 cut(s) 144, 1209, 1295, 2136, 2418, 2955, 3169, 4864
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 9 cut(s) 359, 1063, 1215, 1612, 2080, 2559, 2722, 2836, 2908
BseMI GCAATG 2 cut(s) 1928, 2274
BseRI GAGGAG 4 cut(s) 77, 2225, 2804, 3739
BseSI GKGCMC 2 cut(s) 323, 778
BseXI GCAGC 7 cut(s) 31, 34, 169, 607, 2063, 3146, 4097
BseYI CCCAGC 1 cut(s) 4928
BsgI GTGCAG 2 cut(s) 246, 678
BshFI GGCC 6 cut(s) 5, 2461, 2552, 2579, 4132, 4699
BshNI GGYRCC 1 cut(s) 2030
BsiHKAI GWGCWC 2 cut(s) 1256, 4257
BsiHKCI CYCGRG 3 cut(s) 47, 2207, 4801
BslFI GGGAC 2 cut(s) 785, 4171
BslI CCNNNNNNNGG 9 cut(s) 359, 1063, 1215, 1612, 2080, 2559, 2722, 2836, 2908
BsmFI GGGAC 2 cut(s) 785, 4171
BsmI GAATGC 2 cut(s) 2744, 3874
BsnI GGCC 6 cut(s) 5, 2461, 2552, 2579, 4132, 4699
Bso31I GGTCTC 2 cut(s) 44, 4513
BsoBI CYCGRG 3 cut(s) 47, 2207, 4801
Bsp119I TTCGAA 4 cut(s) 967, 1071, 2280, 4298
Bsp1286I GDGCHC 7 cut(s) 323, 778, 893, 1256, 3963, 4257, 4911
Bsp13I TCCGGA 2 cut(s) 2887, 3861
Bsp1407I TGTACA 3 cut(s) 1153, 2128, 2184
Bsp1720I GCTNAGC 1 cut(s) 3193
Bsp19I CCATGG 2 cut(s) 1209, 4864
BspACI CCGC 4 cut(s) 1142, 2141, 4208, 4580
BspANI GGCC 6 cut(s) 5, 2461, 2552, 2579, 4132, 4699
BspEI TCCGGA 2 cut(s) 2887, 3861
BspHI TCATGA 4 cut(s) 522, 1123, 3622, 3916
BspLI GGNNCC 3 cut(s) 40, 2032, 4908
BspMAI CTGCAG 2 cut(s) 1870, 4115
BspMI ACCTGC 1 cut(s) 1513
BspT104I TTCGAA 4 cut(s) 967, 1071, 2280, 4298
BspT107I GGYRCC 1 cut(s) 2030
BspTI CTTAAG 1 cut(s) 3353
BspTNI GGTCTC 2 cut(s) 44, 4513
BsrDI GCAATG 2 cut(s) 1928, 2274
BsrFI RCCGGY 2 cut(s) 1056, 2715
BsrGI TGTACA 3 cut(s) 1153, 2128, 2184
BssAI RCCGGY 2 cut(s) 1056, 2715
BssECI CCNNGG 8 cut(s) 144, 1209, 1295, 2136, 2418, 2955, 3169, 4864
BssNAI GTATAC 1 cut(s) 1206
BssT1I CCWWGG 7 cut(s) 144, 1209, 2136, 2418, 2955, 3169, 4864
Bst1107I GTATAC 1 cut(s) 1206
Bst2UI CCWGG 4 cut(s) 1296, 2346, 3909, 4329
Bst6I CTCTTC 9 cut(s) 534, 932, 1068, 1944, 4626, 4668, 4761, 4815, 4982
BstAFI CTTAAG 1 cut(s) 3353
BstAPI GCANNNNNTGC 2 cut(s) 2123, 2735
BstAUI TGTACA 3 cut(s) 1153, 2128, 2184
BstBI TTCGAA 4 cut(s) 967, 1071, 2280, 4298
BstC8I GCNNGC 8 cut(s) 96, 1221, 1591, 2229, 2565, 2922, 4274, 4701
BstDSI CCRYGG 2 cut(s) 1209, 4864
BstEII GGTNACC 1 cut(s) 4868
BstH2I RGCGCY 3 cut(s) 1174, 3193, 3792
BstHHI GCGC 4 cut(s) 1173, 2920, 3192, 3791
BstNI CCWGG 4 cut(s) 1296, 2346, 3909, 4329
BstNSI RCATGY 8 cut(s) 237, 989, 1122, 1826, 2135, 3158, 4051, 4196
BstPAI GACNNNNGTC 1 cut(s) 4266
BstPI GGTNACC 1 cut(s) 4868
BstSFI CTRYAG 3 cut(s) 1866, 3084, 4111
BstSLI GKGCMC 2 cut(s) 323, 778
BstV1I GCAGC 7 cut(s) 31, 34, 169, 607, 2063, 3146, 4097
BstV2I GAAGAC 8 cut(s) 1414, 2116, 2222, 2269, 2620, 3905, 3948, 4104
BstZ17I GTATAC 1 cut(s) 1206
BsuRI GGCC 6 cut(s) 5, 2461, 2552, 2579, 4132, 4699
BtgI CCRYGG 2 cut(s) 1209, 4864
BtsI GCAGTG 1 cut(s) 881
BtsIMutI CAGTG 8 cut(s) 659, 881, 1282, 2599, 3297, 3334, 3952, 4457
BveI ACCTGC 1 cut(s) 1513
Cac8I GCNNGC 8 cut(s) 96, 1221, 1591, 2229, 2565, 2922, 4274, 4701
CaiI CAGNNNCTG 3 cut(s) 3908, 4058, 4835
CciI TCATGA 4 cut(s) 522, 1123, 3622, 3916
CfoI GCGC 4 cut(s) 1173, 2920, 3192, 3791
Cfr10I RCCGGY 2 cut(s) 1056, 2715
Cfr13I GGNCC 8 cut(s) 4, 350, 421, 2460, 2551, 2713, 3073, 3562
CseI GACGC 2 cut(s) 80, 2556
CsiI ACCWGGT 2 cut(s) 2344, 4327
CspCI CAANNNNNGTGG 2 cut(s) 4063, 4098
DraI TTTAAA 1 cut(s) 849
DraIII CACNNNGTG 1 cut(s) 4979
DrdI GACNNNNNNGTC 2 cut(s) 77, 4165
DriI GACNNNNNGTC 2 cut(s) 718, 4329
DseDI GACNNNNNNGTC 2 cut(s) 77, 4165
Eam1104I CTCTTC 9 cut(s) 534, 932, 1068, 1944, 4626, 4668, 4761, 4815, 4982
Eam1105I GACNNNNNGTC 2 cut(s) 718, 4329
EarI CTCTTC 9 cut(s) 534, 932, 1068, 1944, 4626, 4668, 4761, 4815, 4982
Ecl136II GAGCTC 1 cut(s) 4255
Eco130I CCWWGG 7 cut(s) 144, 1209, 2136, 2418, 2955, 3169, 4864
Eco147I AGGCCT 1 cut(s) 4699
Eco24I GRGCYC 4 cut(s) 893, 3963, 4257, 4911
Eco31I GGTCTC 2 cut(s) 44, 4513
Eco32I GATATC 3 cut(s) 610, 3550, 4093
Eco47I GGWCC 5 cut(s) 350, 421, 2713, 3073, 3562
Eco47III AGCGCT 2 cut(s) 3191, 3790
Eco53kI GAGCTC 1 cut(s) 4255
Eco57I CTGAAG 7 cut(s) 1044, 3138, 3885, 4710, 4746, 4857, 4904
Eco88I CYCGRG 3 cut(s) 47, 2207, 4801
Eco91I GGTNACC 1 cut(s) 4868
EcoICRI GAGCTC 1 cut(s) 4255
EcoO109I RGGNCCY 1 cut(s) 3073
EcoO65I GGTNACC 1 cut(s) 4868
EcoRI GAATTC 1 cut(s) 3148
EcoRII CCWGG 4 cut(s) 1294, 2344, 3907, 4327
EcoRV GATATC 3 cut(s) 610, 3550, 4093
EcoT14I CCWWGG 7 cut(s) 144, 1209, 2136, 2418, 2955, 3169, 4864
EcoT38I GRGCYC 4 cut(s) 893, 3963, 4257, 4911
ErhI CCWWGG 7 cut(s) 144, 1209, 2136, 2418, 2955, 3169, 4864
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 2 cut(s) 785, 4171
FbaI TGATCA 3 cut(s) 484, 829, 1639
FblI GTMKAC 2 cut(s) 1205, 2476
Fnu4HI GCNGC 8 cut(s) 20, 23, 158, 621, 2052, 2142, 3135, 4111
FriOI GRGCYC 4 cut(s) 893, 3963, 4257, 4911
Fsp4HI GCNGC 8 cut(s) 20, 23, 158, 621, 2052, 2142, 3135, 4111
GlaI GCGC 4 cut(s) 1172, 2919, 3191, 3790
GluI GCNGC 8 cut(s) 20, 23, 158, 621, 2052, 2142, 3135, 4111
GsaI CCCAGC 1 cut(s) 4932
GsuI CTGGAG 6 cut(s) 117, 401, 834, 1490, 1862, 4725
HaeII RGCGCY 3 cut(s) 1174, 3193, 3792
HaeIII GGCC 6 cut(s) 5, 2461, 2552, 2579, 4132, 4699
HgaI GACGC 2 cut(s) 80, 2556
HhaI GCGC 4 cut(s) 1173, 2920, 3192, 3791
Hin6I GCGC 4 cut(s) 1171, 2918, 3190, 3789
HinP1I GCGC 4 cut(s) 1171, 2918, 3190, 3789
HincII GTYRAC 6 cut(s) 723, 859, 945, 3225, 4261, 4747
HindII GTYRAC 6 cut(s) 723, 859, 945, 3225, 4261, 4747
HindIII AAGCTT 4 cut(s) 218, 665, 2970, 4754
HpaI GTTAAC 1 cut(s) 859
Hpy166II GTNNAC 9 cut(s) 723, 859, 945, 1206, 2477, 3225, 4261, 4585, 4747
Hpy8I GTNNAC 9 cut(s) 723, 859, 945, 1206, 2477, 3225, 4261, 4585, 4747
HpyCH4IV ACGT 2 cut(s) 3311, 3886
HpySE526I ACGT 2 cut(s) 3311, 3886
HspAI GCGC 4 cut(s) 1171, 2918, 3190, 3789
Kpn2I TCCGGA 2 cut(s) 2887, 3861
KpnI GGTACC 1 cut(s) 2034
Ksp22I TGATCA 3 cut(s) 484, 829, 1639
KspAI GTTAAC 1 cut(s) 859
LmnI GCTCC 9 cut(s) 90, 277, 1033, 1471, 1549, 1819, 3698, 4252, 4906
Lsp1109I GCAGC 7 cut(s) 31, 34, 169, 607, 2063, 3146, 4097
MabI ACCWGGT 2 cut(s) 2344, 4327
MaeII ACGT 2 cut(s) 3311, 3886
MfeI CAATTG 4 cut(s) 1344, 1643, 2541, 2993
MhlI GDGCHC 7 cut(s) 323, 778, 893, 1256, 3963, 4257, 4911
MlyI GAGTC 9 cut(s) 158, 379, 680, 1373, 3331, 3845, 4580, 4658, 4832
MmeI TCCRAC 4 cut(s) 99, 847, 1044, 3024
MroI TCCGGA 2 cut(s) 2887, 3861
MroXI GAANNNNTTC 6 cut(s) 909, 1353, 1626, 1836, 2681, 4227
MslI CAYNNNNRTG 3 cut(s) 1533, 3027, 3310
MspA1I CMGCKG 1 cut(s) 835
MspCI CTTAAG 1 cut(s) 3353
MunI CAATTG 4 cut(s) 1344, 1643, 2541, 2993
Mva1269I GAATGC 2 cut(s) 2744, 3874
MvaI CCWGG 4 cut(s) 1296, 2346, 3909, 4329
NciI CCSGG 6 cut(s) 253, 888, 2039, 2463, 3566, 3636
NcoI CCATGG 2 cut(s) 1209, 4864
NlaIV GGNNCC 3 cut(s) 40, 2032, 4908
NmeAIII GCCGAG 1 cut(s) 1192
NmuCI GTSAC 8 cut(s) 126, 442, 1052, 1512, 3164, 3953, 4321, 4868
NspI RCATGY 8 cut(s) 237, 989, 1122, 1826, 2135, 3158, 4051, 4196
NspV TTCGAA 4 cut(s) 967, 1071, 2280, 4298
OliI CACNNNNGTG 1 cut(s) 3310
PaeR7I CTCGAG 3 cut(s) 47, 2207, 4801
PagI TCATGA 4 cut(s) 522, 1123, 3622, 3916
PaqCI CACCTGC 1 cut(s) 1513
PceI AGGCCT 1 cut(s) 4699
PciI ACATGT 2 cut(s) 233, 985
PctI GAATGC 2 cut(s) 2744, 3874
PdmI GAANNNNTTC 6 cut(s) 909, 1353, 1626, 1836, 2681, 4227
PflMI CCANNNNNTGG 1 cut(s) 359
PfoI TCCNGGA 2 cut(s) 2037, 3564
PkrI GCNGC 8 cut(s) 21, 24, 159, 622, 2053, 2143, 3136, 4112
PleI GAGTC 9 cut(s) 157, 379, 679, 1372, 3331, 3844, 4580, 4658, 4831
PpsI GAGTC 9 cut(s) 157, 379, 679, 1372, 3331, 3844, 4580, 4658, 4831
PpuMI RGGWCCY 1 cut(s) 3073
PscI ACATGT 2 cut(s) 233, 985
PshAI GACNNNNGTC 1 cut(s) 4266
PsiI TTATAA 2 cut(s) 2312, 3455
Psp124BI GAGCTC 1 cut(s) 4257
Psp5II RGGWCCY 1 cut(s) 3073
Psp6I CCWGG 4 cut(s) 1294, 2344, 3907, 4327
PspEI GGTNACC 1 cut(s) 4868
PspFI CCCAGC 1 cut(s) 4928
PspGI CCWGG 4 cut(s) 1294, 2344, 3907, 4327
PspN4I GGNNCC 3 cut(s) 40, 2032, 4908
PspPI GGNCC 8 cut(s) 4, 350, 421, 2460, 2551, 2713, 3073, 3562
PspPPI RGGWCCY 1 cut(s) 3073
PspXI VCTCGAGB 1 cut(s) 2207
PsrI GAACNNNNNNTAC 2 cut(s) 4339, 4371
PstI CTGCAG 2 cut(s) 1870, 4115
PstNI CAGNNNCTG 3 cut(s) 3908, 4058, 4835
PvuII CAGCTG 1 cut(s) 835
RseI CAYNNNNRTG 3 cut(s) 1533, 3027, 3310
SacI GAGCTC 1 cut(s) 4257
SatI GCNGC 8 cut(s) 20, 23, 158, 621, 2052, 2142, 3135, 4111
Sau96I GGNCC 8 cut(s) 4, 350, 421, 2460, 2551, 2713, 3073, 3562
ScaI AGTACT 1 cut(s) 1780
SchI GAGTC 9 cut(s) 158, 379, 680, 1373, 3331, 3845, 4580, 4658, 4832
SduI GDGCHC 7 cut(s) 323, 778, 893, 1256, 3963, 4257, 4911
SexAI ACCWGGT 2 cut(s) 2344, 4327
SfcI CTRYAG 3 cut(s) 1866, 3084, 4111
Sfr274I CTCGAG 3 cut(s) 47, 2207, 4801
SfuI TTCGAA 4 cut(s) 967, 1071, 2280, 4298
SinI GGWCC 5 cut(s) 350, 421, 2713, 3073, 3562
SlaI CTCGAG 3 cut(s) 47, 2207, 4801
SmiMI CAYNNNNRTG 3 cut(s) 1533, 3027, 3310
SseBI AGGCCT 1 cut(s) 4699
SsiI CCGC 4 cut(s) 1142, 2141, 4208, 4580
SspI AATATT 6 cut(s) 1900, 2487, 2944, 3298, 4492, 4657
SstI GAGCTC 1 cut(s) 4257
StuI AGGCCT 1 cut(s) 4699
StyI CCWWGG 7 cut(s) 144, 1209, 2136, 2418, 2955, 3169, 4864
TaiI ACGT 2 cut(s) 3314, 3889
TauI GCSGC 1 cut(s) 2144
TscAI CASTG 8 cut(s) 659, 888, 1282, 2606, 3297, 3334, 3952, 4457
TseFI GTSAC 8 cut(s) 126, 442, 1052, 1512, 3164, 3953, 4321, 4868
TseI GCWGC 7 cut(s) 19, 22, 157, 620, 2051, 3134, 4110
Tsp45I GTSAC 8 cut(s) 126, 442, 1052, 1512, 3164, 3953, 4321, 4868
TspGWI ACGGA 3 cut(s) 1777, 3736, 4967
TspRI CASTG 8 cut(s) 659, 888, 1282, 2606, 3297, 3334, 3952, 4457
Van91I CCANNNNNTGG 1 cut(s) 359
Vha464I CTTAAG 1 cut(s) 3353
VpaK11BI GGWCC 5 cut(s) 350, 421, 2713, 3073, 3562
XceI RCATGY 8 cut(s) 237, 989, 1122, 1826, 2135, 3158, 4051, 4196
XcmI CCANNNNNNNNNTGG 1 cut(s) 1216
XhoI CTCGAG 3 cut(s) 47, 2207, 4801
XmiI GTMKAC 2 cut(s) 1205, 2476
XmnI GAANNNNTTC 6 cut(s) 909, 1353, 1626, 1836, 2681, 4227
ZrmI AGTACT 1 cut(s) 1780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.