FvH4_3g00620

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010647
Physical Location & Seq
Forward (+)
307337 .. 309017
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00620.t1

Sequence Viewer

Length: 1266 bp
ATGGCGGTGTCTGATGAGAGTAATAGATATTGCCTTTATGCATGCATGTCGTTGCTTCTAGGGTTTGGCCTCTTTGCAGATTGCTCGAGTTCGGTCGTTGATGACCCAGCAGGGCAAATTGGTTCCATAGGCAACAACTGCTCTGAAAGAGAAACCACGAGGAAGCTGCTGCTCCAAGTCCTCCGACCCTCATGTCGTAAAACCGGAAATTCCATCGATGACTGCTGGCGTTGTGATCCCAACTGGTTCAACAACCGAAAGCGTCTCGCGGACTGCGCCATTGGTTTTGGCAAAGACGCCAAGGGCGGCCGCGATGGACACATTTACGTTGTCACCGACTCGAGTGACAACGATGTTGTAAACCCTAGACCAAGCACTCTCCGCTACGCTGTCATCCAGACCGAGCCTCTCTGGATCATATTCGCCAGGGACATGCAGATCACACTGAAGCAGGAGCTGATCATGAATAGCTTCAAGACCATTGACGGGCGCGGAGTCAAAGTTGAAATTGCTTACGGAGGGCAAATAACGGTTCAATATGTCACAAACATTATAATCCATGGTCTGCACATCCACCACTGCAAAGAGTTACGCAATGCAATGGTGAGAGACTCCCCATCTCATTATGGATGGAGAGCAAAATCTGATGGCGATGGCATCTCCATCTTCGGAGCGAAACACATATGGATCGATCACAATTCACTCTCAAACTGTACCGACGGCCTTGTGGATGCCGTGGAGGCCTCAACTGCAATAACCATTTCCAACAATCTATTTATGGACCACGATAAGGTGATGCTGTTAGGGGCCCAAGATGGTTATACTCAAGACAAGGTGATGCAAGTGACTATTGTTTACAACCGTTTCGGGCCGGGAGTAGAGCAGAGAATGCCTCATGCTAGGTACGGCAATTTCCATATAGTGAACAATGACTACGCTAACGGGTGGAAGATATATGCCGTTGGGGGATCTTCAAACCCTACCATCAACAGCGAAGGCAACAGATACGTTGCCGACCCGGTAAAGTATCCCTATGCGAAGGAGGTTACCCAGAGAATGAAGGCGAGCGAATCAGAGTGGAAGAAATGGAATTGGAGGTCGAAGGGAGATCAAGTCTTGAATGGTGCTTACTTCATTCCGTCCGGAGATGCTAGTCCGGCAGCCTATGTCCAGGCCTCTAGCGCCTTACCCAAGCCAGCTTCCATGGTTTTAGCCATGACGGCTAATGCTGGTGCACTTACTTGCCTCAGAGGTCATGTTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

422

Amino Acids

46.57

Weight (kDa)

7.92

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 152 - 337 1.1e-19 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AasI GACNNNNNNGTC 1 cut(s) 192
AccII CGCG 3 cut(s) 269, 312, 492
AccIII TCCGGA 1 cut(s) 1142
AciI CCGC 6 cut(s) 5, 269, 306, 310, 382, 492
AclWI GGATC 4 cut(s) 230, 422, 695, 976
AcoI YGGCCR 1 cut(s) 307
AcsI RAATTY 1 cut(s) 208
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 2 cut(s) 715, 905
AfiI CCNNNNNNNGG 2 cut(s) 486, 790
AgsI TTSAA 6 cut(s) 250, 475, 506, 536, 975, 1120
AjnI CCWGG 2 cut(s) 425, 1170
AluBI AGCT 4 cut(s) 166, 457, 471, 1199
AluI AGCT 4 cut(s) 166, 457, 471, 1199
Alw21I GWGCWC 1 cut(s) 1237
Alw26I GTCTC 2 cut(s) 269, 603
Alw44I GTGCAC 1 cut(s) 1233
AlwI GGATC 4 cut(s) 230, 422, 695, 976
AlwNI CAGNNNCTG 1 cut(s) 457
Ama87I CYCGRG 2 cut(s) 85, 340
Aor13HI TCCGGA 1 cut(s) 1142
AoxI GGCC 7 cut(s) 67, 307, 721, 741, 807, 869, 1173
ApaI GGGCCC 1 cut(s) 811
ApaLI GTGCAC 1 cut(s) 1233
ApeKI GCWGC 3 cut(s) 166, 169, 1160
ApoI RAATTY 1 cut(s) 208
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 3 cut(s) 278, 492, 1184
AspS9I GGNCC 4 cut(s) 781, 807, 808, 869
AsuC2I CCSGG 2 cut(s) 873, 1019
AsuHPI GGTGA 4 cut(s) 325, 616, 805, 847
AvaI CYCGRG 2 cut(s) 85, 340
AvaII GGWCC 1 cut(s) 781
BaeGI GKGCMC 2 cut(s) 811, 1237
BanII GRGCYC 1 cut(s) 811
BauI CACGAG 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 1237
BbvI GCAGC 3 cut(s) 153, 156, 1172
BccI CCATC 9 cut(s) 221, 308, 624, 625, 641, 647, 671, 809, 992
BceAI ACGGC 5 cut(s) 719, 736, 922, 944, 1236
BcgI CGANNNNNNTGC 6 cut(s) 30, 64, 66, 100, 148, 182
BciT130I CCWGG 2 cut(s) 427, 1172
BciVI GTATCC 1 cut(s) 1038
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 2 cut(s) 873, 1019
BcoDI GTCTC 2 cut(s) 269, 603
BfaI CTAG 5 cut(s) 59, 366, 900, 1152, 1179
BfoI RGCGCY 1 cut(s) 1185
BfuI GTATCC 1 cut(s) 1038
BglI GCCNNNNNGGC 2 cut(s) 740, 1220
BisI GCNGC 5 cut(s) 167, 170, 307, 310, 1161
BlsI GCNGC 5 cut(s) 168, 171, 308, 311, 1162
Bme1390I CCNGG 4 cut(s) 427, 873, 1019, 1172
Bme18I GGWCC 1 cut(s) 781
BmeT110I CYCGRG 2 cut(s) 85, 340
BmgT120I GGNCC 4 cut(s) 781, 807, 808, 869
BmiI GGNNCC 3 cut(s) 124, 808, 809
BmrFI CCNGG 4 cut(s) 427, 873, 1019, 1172
BmsI GCATC 5 cut(s) 666, 721, 786, 828, 1138
BplI GAGNNNNNCTC 2 cut(s) 877, 909
BpuEI CTTGAG 1 cut(s) 810
BpuMI CCSGG 2 cut(s) 873, 1019
Bsa29I ATCGAT 2 cut(s) 216, 690
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 5 cut(s) 300, 426, 559, 735, 1203
BsaWI WCCGGW 2 cut(s) 203, 1142
BsaXI ACNNNNNCTCC 4 cut(s) 486, 516, 1098, 1128
Bsc4I CCNNNNNNNGG 2 cut(s) 486, 790
Bse1I ACTGG 1 cut(s) 248
Bse3DI GCAATG 2 cut(s) 601, 606
BseAI TCCGGA 1 cut(s) 1142
BseBI CCWGG 2 cut(s) 427, 1172
BseCI ATCGAT 2 cut(s) 216, 690
BseDI CCNNGG 5 cut(s) 300, 426, 559, 735, 1203
BseGI GGATG 4 cut(s) 393, 570, 635, 736
BseLI CCNNNNNNNGG 2 cut(s) 486, 790
BseMI GCAATG 2 cut(s) 601, 606
BseMII CTCAG 1 cut(s) 1261
BseNI ACTGG 1 cut(s) 248
BseSI GKGCMC 2 cut(s) 811, 1237
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 3 cut(s) 153, 156, 1172
BseYI CCCAGC 1 cut(s) 106
BsgI GTGCAG 1 cut(s) 551
Bsh1236I CGCG 3 cut(s) 269, 312, 492
Bsh1285I CGRYCG 2 cut(s) 96, 310
BshFI GGCC 7 cut(s) 69, 309, 723, 743, 809, 871, 1175
BshVI ATCGAT 2 cut(s) 216, 690
BsiEI CGRYCG 2 cut(s) 96, 310
BsiHKAI GWGCWC 1 cut(s) 1237
BsiHKCI CYCGRG 2 cut(s) 85, 340
BsiSI CCGG 5 cut(s) 204, 872, 1019, 1143, 1157
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 2 cut(s) 486, 790
BsmAI GTCTC 2 cut(s) 269, 603
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 443
BsmI GAATGC 1 cut(s) 894
BsnI GGCC 7 cut(s) 69, 309, 723, 743, 809, 871, 1175
BsoBI CYCGRG 2 cut(s) 85, 340
Bsp120I GGGCCC 1 cut(s) 807
Bsp1286I GDGCHC 2 cut(s) 811, 1237
Bsp13I TCCGGA 1 cut(s) 1142
Bsp143I GATC 8 cut(s) 235, 414, 438, 459, 687, 691, 968, 1108
Bsp19I CCATGG 2 cut(s) 559, 1203
BspACI CCGC 6 cut(s) 5, 269, 306, 310, 382, 492
BspANI GGCC 7 cut(s) 69, 309, 723, 743, 809, 871, 1175
BspCNI CTCAG 1 cut(s) 1260
BspDI ATCGAT 2 cut(s) 216, 690
BspEI TCCGGA 1 cut(s) 1142
BspFNI CGCG 3 cut(s) 269, 312, 492
BspHI TCATGA 1 cut(s) 462
BspLI GGNNCC 3 cut(s) 124, 808, 809
BspPI GGATC 4 cut(s) 230, 422, 695, 976
BsrDI GCAATG 2 cut(s) 601, 606
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 5 cut(s) 300, 426, 559, 735, 1203
BssMI GATC 8 cut(s) 235, 414, 438, 459, 687, 691, 968, 1108
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 157
BssT1I CCWWGG 3 cut(s) 300, 559, 1203
Bst2BI CACGAG 1 cut(s) 157
Bst2UI CCWGG 2 cut(s) 427, 1172
Bst4CI ACNGT 3 cut(s) 532, 713, 863
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 2 cut(s) 138, 889
BstC8I GCNNGC 4 cut(s) 43, 227, 1066, 1197
BstDEI CTNAG 1 cut(s) 1247
BstDSI CCRYGG 3 cut(s) 559, 735, 1203
BstEII GGTNACC 1 cut(s) 1045
BstF5I GGATG 4 cut(s) 393, 570, 635, 736
BstFNI CGCG 3 cut(s) 269, 312, 492
BstH2I RGCGCY 1 cut(s) 1185
BstHHI GCGC 3 cut(s) 278, 492, 1184
BstKTI GATC 8 cut(s) 238, 417, 441, 462, 690, 694, 971, 1111
BstMAI GTCTC 2 cut(s) 269, 603
BstMBI GATC 8 cut(s) 235, 414, 438, 459, 687, 691, 968, 1108
BstMCI CGRYCG 2 cut(s) 96, 310
BstMWI GCNNNNNNNGC 9 cut(s) 138, 275, 381, 740, 749, 889, 1157, 1181, 1220
BstNI CCWGG 2 cut(s) 427, 1172
BstNSI RCATGY 3 cut(s) 45, 49, 436
BstPI GGTNACC 1 cut(s) 1045
BstSCI CCNGG 4 cut(s) 425, 871, 1017, 1170
BstSLI GKGCMC 2 cut(s) 811, 1237
BstUI CGCG 3 cut(s) 269, 312, 492
BstV1I GCAGC 3 cut(s) 153, 156, 1172
BstX2I RGATCY 1 cut(s) 968
BstYI RGATCY 1 cut(s) 968
BstZI CGGCCG 1 cut(s) 307
Bsu15I ATCGAT 2 cut(s) 216, 690
BsuI GTATCC 1 cut(s) 1038
BsuRI GGCC 7 cut(s) 69, 309, 723, 743, 809, 871, 1175
BsuTUI ATCGAT 2 cut(s) 216, 690
BtgI CCRYGG 3 cut(s) 559, 735, 1203
BtgZI GCGATG 2 cut(s) 327, 666
BtsCI GGATG 4 cut(s) 393, 570, 635, 736
BtsI GCAGTG 1 cut(s) 577
BtsIMutI CAGTG 2 cut(s) 443, 577
Cac8I GCNNGC 4 cut(s) 43, 227, 1066, 1197
CaiI CAGNNNCTG 1 cut(s) 457
CciI TCATGA 1 cut(s) 462
CciNI GCGGCCGC 1 cut(s) 307
CfoI GCGC 3 cut(s) 278, 492, 1184
Cfr13I GGNCC 4 cut(s) 781, 807, 808, 869
ClaI ATCGAT 2 cut(s) 216, 690
CseI GACGC 2 cut(s) 251, 305
Csp6I GTAC 2 cut(s) 714, 904
CviQI GTAC 2 cut(s) 714, 904
DdeI CTNAG 1 cut(s) 1247
DpnI GATC 8 cut(s) 237, 416, 440, 461, 689, 693, 970, 1110
DpnII GATC 8 cut(s) 235, 414, 438, 459, 687, 691, 968, 1108
DrdI GACNNNNNNGTC 1 cut(s) 192
DseDI GACNNNNNNGTC 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 307
EagI CGGCCG 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 3 cut(s) 300, 559, 1203
Eco147I AGGCCT 2 cut(s) 743, 1175
Eco24I GRGCYC 1 cut(s) 811
Eco47I GGWCC 1 cut(s) 781
Eco52I CGGCCG 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 467
Eco88I CYCGRG 2 cut(s) 85, 340
Eco91I GGTNACC 1 cut(s) 1045
EcoO109I RGGNCCY 1 cut(s) 807
EcoO65I GGTNACC 1 cut(s) 1045
EcoRII CCWGG 2 cut(s) 425, 1170
EcoT14I CCWWGG 3 cut(s) 300, 559, 1203
EcoT22I ATGCAT 2 cut(s) 43, 47
EcoT38I GRGCYC 1 cut(s) 811
ErhI CCWWGG 3 cut(s) 300, 559, 1203
Esp3I CGTCTC 1 cut(s) 269
FaqI GGGAC 1 cut(s) 443
FauNDI CATATG 1 cut(s) 683
FbaI TGATCA 1 cut(s) 459
Fnu4HI GCNGC 5 cut(s) 167, 170, 307, 310, 1161
FokI GGATG 4 cut(s) 380, 557, 642, 743
FriOI GRGCYC 1 cut(s) 811
Fsp4HI GCNGC 5 cut(s) 167, 170, 307, 310, 1161
FspBI CTAG 5 cut(s) 59, 366, 900, 1152, 1179
GlaI GCGC 3 cut(s) 277, 491, 1183
GluI GCNGC 5 cut(s) 167, 170, 307, 310, 1161
GsaI CCCAGC 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 1185
HaeIII GGCC 7 cut(s) 69, 309, 723, 743, 809, 871, 1175
HapII CCGG 5 cut(s) 204, 872, 1019, 1143, 1157
HgaI GACGC 2 cut(s) 251, 305
HhaI GCGC 3 cut(s) 278, 492, 1184
Hin1I GRCGYC 1 cut(s) 297
Hin6I GCGC 3 cut(s) 276, 490, 1182
HinP1I GCGC 3 cut(s) 276, 490, 1182
HinfI GANTC 4 cut(s) 338, 495, 611, 1070
HpaII CCGG 5 cut(s) 204, 872, 1019, 1143, 1157
HphI GGTGA 4 cut(s) 325, 616, 805, 847
Hpy166II GTNNAC 4 cut(s) 361, 856, 925, 1235
Hpy188I TCNGA 7 cut(s) 13, 145, 185, 646, 671, 1075, 1250
Hpy188III TCNNGA 7 cut(s) 397, 412, 463, 475, 827, 1117, 1143
Hpy8I GTNNAC 4 cut(s) 361, 856, 925, 1235
Hpy99I CGWCG 1 cut(s) 722
HpyAV CCTTC 4 cut(s) 989, 1033, 1054, 1096
HpyCH4III ACNGT 3 cut(s) 532, 713, 863
HpyCH4IV ACGT 2 cut(s) 327, 1008
HpyF10VI GCNNNNNNNGC 9 cut(s) 138, 275, 381, 740, 749, 889, 1157, 1181, 1220
HpyF3I CTNAG 1 cut(s) 1247
HpySE526I ACGT 2 cut(s) 327, 1008
Hsp92I GRCGYC 1 cut(s) 297
HspAI GCGC 3 cut(s) 276, 490, 1182
Kpn2I TCCGGA 1 cut(s) 1142
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 8 cut(s) 235, 414, 438, 459, 687, 691, 968, 1108
LmnI GCTCC 3 cut(s) 177, 454, 671
Lsp1109I GCAGC 3 cut(s) 153, 156, 1172
LweI GCATC 5 cut(s) 666, 721, 786, 828, 1138
MaeI CTAG 5 cut(s) 59, 366, 900, 1152, 1179
MaeII ACGT 2 cut(s) 327, 1008
MaeIII GTNAC 6 cut(s) 331, 344, 541, 588, 844, 1045
MalI GATC 8 cut(s) 237, 416, 440, 461, 689, 693, 970, 1110
MboI GATC 8 cut(s) 235, 414, 438, 459, 687, 691, 968, 1108
MboII GAAGA 4 cut(s) 658, 961, 963, 1093
MflI RGATCY 1 cut(s) 968
MhlI GDGCHC 2 cut(s) 811, 1237
MluCI AATT 6 cut(s) 117, 208, 507, 697, 910, 1090
MlyI GAGTC 3 cut(s) 332, 504, 605
MmeI TCCRAC 2 cut(s) 208, 789
Mph1103I ATGCAT 2 cut(s) 43, 47
MroI TCCGGA 1 cut(s) 1142
MroXI GAANNNNTTC 1 cut(s) 470
MspI CCGG 5 cut(s) 204, 872, 1019, 1143, 1157
MspR9I CCNGG 4 cut(s) 427, 873, 1019, 1172
Mva1269I GAATGC 1 cut(s) 894
MvaI CCWGG 2 cut(s) 427, 1172
MvnI CGCG 3 cut(s) 269, 312, 492
MwoI GCNNNNNNNGC 9 cut(s) 138, 275, 381, 740, 749, 889, 1157, 1181, 1220
NciI CCSGG 2 cut(s) 873, 1019
NcoI CCATGG 2 cut(s) 559, 1203
NdeI CATATG 1 cut(s) 683
NdeII GATC 8 cut(s) 235, 414, 438, 459, 687, 691, 968, 1108
NlaIV GGNNCC 3 cut(s) 124, 808, 809
NmuCI GTSAC 4 cut(s) 331, 344, 541, 844
NotI GCGGCCGC 1 cut(s) 307
NsiI ATGCAT 2 cut(s) 43, 47
NspI RCATGY 3 cut(s) 45, 49, 436
PaeI GCATGC 1 cut(s) 45
PaeR7I CTCGAG 2 cut(s) 85, 340
PagI TCATGA 1 cut(s) 462
PceI AGGCCT 2 cut(s) 743, 1175
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PctI GAATGC 1 cut(s) 894
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 1 cut(s) 1070
PkrI GCNGC 5 cut(s) 168, 171, 308, 311, 1162
PleI GAGTC 3 cut(s) 332, 503, 605
PpsI GAGTC 3 cut(s) 332, 503, 605
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 2 cut(s) 425, 1170
PspEI GGTNACC 1 cut(s) 1045
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 2 cut(s) 425, 1170
PspN4I GGNNCC 3 cut(s) 124, 808, 809
PspOMI GGGCCC 1 cut(s) 807
PspPI GGNCC 4 cut(s) 781, 807, 808, 869
PspXI VCTCGAGB 2 cut(s) 85, 340
PsrI GAACNNNNNNTAC 2 cut(s) 917, 949
PstNI CAGNNNCTG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 968
RsaI GTAC 2 cut(s) 715, 905
RsaNI GTAC 2 cut(s) 714, 904
SatI GCNGC 5 cut(s) 167, 170, 307, 310, 1161
Sau3AI GATC 8 cut(s) 235, 414, 438, 459, 687, 691, 968, 1108
Sau96I GGNCC 4 cut(s) 781, 807, 808, 869
SchI GAGTC 3 cut(s) 332, 504, 605
ScrFI CCNGG 4 cut(s) 427, 873, 1019, 1172
SduI GDGCHC 2 cut(s) 811, 1237
SfaNI GCATC 5 cut(s) 666, 721, 786, 828, 1138
Sfr274I CTCGAG 2 cut(s) 85, 340
SinI GGWCC 1 cut(s) 781
SlaI CTCGAG 2 cut(s) 85, 340
SmlI CTYRAG 3 cut(s) 85, 340, 825
SmoI CTYRAG 3 cut(s) 85, 340, 825
SphI GCATGC 1 cut(s) 45
Sse9I AATT 6 cut(s) 117, 208, 507, 697, 910, 1090
SseBI AGGCCT 2 cut(s) 743, 1175
SsiI CCGC 6 cut(s) 5, 269, 306, 310, 382, 492
SspMI CTAG 5 cut(s) 59, 366, 900, 1152, 1179
StuI AGGCCT 2 cut(s) 743, 1175
StyD4I CCNGG 4 cut(s) 425, 871, 1017, 1170
StyI CCWWGG 3 cut(s) 300, 559, 1203
TaaI ACNGT 3 cut(s) 532, 713, 863
TaiI ACGT 2 cut(s) 330, 1011
TaqI TCGA 5 cut(s) 86, 216, 341, 690, 1100
TaqII GACCGA 2 cut(s) 82, 416
TasI AATT 6 cut(s) 117, 208, 507, 697, 910, 1090
TauI GCSGC 2 cut(s) 309, 312
TfiI GAWTC 1 cut(s) 1070
TscAI CASTG 2 cut(s) 450, 584
TseFI GTSAC 4 cut(s) 331, 344, 541, 844
TseI GCWGC 3 cut(s) 166, 169, 1160
Tsp45I GTSAC 4 cut(s) 331, 344, 541, 844
TspDTI ATGAA 3 cut(s) 479, 1073, 1123
TspGWI ACGGA 2 cut(s) 531, 1128
TspRI CASTG 2 cut(s) 450, 584
VneI GTGCAC 1 cut(s) 1233
VpaK11BI GGWCC 1 cut(s) 781
XapI RAATTY 1 cut(s) 208
XceI RCATGY 3 cut(s) 45, 49, 436
XhoI CTCGAG 2 cut(s) 85, 340
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 5 cut(s) 59, 366, 900, 1152, 1179
Zsp2I ATGCAT 2 cut(s) 43, 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.