FvH4_6g02350

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010647
Physical Location & Seq
Forward (+)
1359132 .. 1360736
1605 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02350.t1

Sequence Viewer

Length: 1284 bp
ATGGCGGTGTTCGAGTGGAGCAAGAGAATGATTTGTTCGTGTGTATTGGCTCTCTTCGTGTTCAGCTTCATTGCCGAAACACGAACTGCACCAACAGCACTCGATGACCCCGAGGAAGTTGCTTCCATGGTCTCCATTATCATCCGAAACAGCACTGAAAGAAAGCTCATGCAACTCCGGGGGTCACCCTCATCATGTGTAACCGGGAATGCCATCGACGACTGTTGGCGATGTGACACCAACTGGTTAAACAATCGCAAGCGCCTCGCGGCCTGCGCCATTGGTTTCGGCAGGAACGCAATCGGCGGCCGCGACGGGCAGTTCTACGTTGTCACTGACCCTAGTGACAATGATGCCGTCAACCCTAGAGCTGGAACTATTCGTCATGCTGTAATACAAACCGAGCCCTTATGGATTGTGTTCCAGCGTGACATGGTGATCCAGCTCAAGCAGGAGCTCATTATGAACAGCTATAAGACCATTGACGGCCGAGGAGCCAATGTTCACATTGCGAATGGAGCATGCATTACGATCCAAAACATCAAGCACATCATAATACATGGCCTCCATATCCATGGCTGCAGGCCTACGGGGAATGCCTTGGTGAGGAGCACACCGTCACACTTTGGTTGGAGAGGAATGGCAGATGGTGACGCCGTGTCGATTTTCGGGTCCAGGGACGTGTGGATTGATCACAACTCGCTATCCAAGTGTGCCGACGGCCTCGTGGATGTTGTCATGGCCTCAACTGCAGTCACAATCTCGAACAATGACTTATGGCACCACAATGAGGTCATGTTGTTTGGTCACAGCAACTCTTATAAGGGAGACAAGGTCATGCAAGTGACTGTTGCTTACAATCACTTTGGGGAAGGACTACGGCAAAGGATGCCACATTGTAGGCAAGGTTATTGTCATGTGGTGAACAATGACTACAGCAGTGGCTGGGGAATATATGCCATCGGCGGAAGTGCAAACCCTACCATCAATAGCGAAGGCAACCGATTTGTGGCTGATCCGAAAAGGTACCCTTATTCCAATCAGGTTACCAAGAGGACATCAGGTGACAAGTCGGAGTGGAAGAACTGGAATTGGAGGTCTAAAGGAGATTTGTTCTTGAATGGTGCCTACTTTGTTGAGTCTGGGGCAACCGGAGCTAACTCGGAGGCCTATGCTAAGGCCTCGAGCGATGTCAAAGCTCAGAAATCATCCAAAGTCAGAGCCATAACTTCTGCTGCCGGCGCACTCGTCTGCCGGAGAAACCGTATGTGCACTAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

428

Amino Acids

47.22

Weight (kDa)

9.13

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 150 - 357 1.1e-18 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 822
Acc65I GGTACC 1 cut(s) 1026
AccB1I GGYRCC 3 cut(s) 780, 1026, 1124
AccII CGCG 2 cut(s) 269, 312
AciI CCGC 5 cut(s) 5, 269, 306, 310, 966
AclWI GGATC 3 cut(s) 433, 526, 1010
AcoI YGGCCR 2 cut(s) 307, 487
AcyI GRCGYC 1 cut(s) 654
AfaI GTAC 1 cut(s) 1028
AfiI CCNNNNNNNGG 4 cut(s) 371, 606, 790, 1009
AflIII ACRYGT 1 cut(s) 681
AgsI TTSAA 1 cut(s) 1120
AhdI GACNNNNNGTC 1 cut(s) 658
AjiI CACGTC 1 cut(s) 682
AjnI CCWGG 1 cut(s) 674
AloI GAACNNNNNNTCC 2 cut(s) 486, 518
AluBI AGCT 8 cut(s) 66, 166, 371, 445, 457, 471, 1157, 1199
AluI AGCT 8 cut(s) 66, 166, 371, 445, 457, 471, 1157, 1199
Alw21I GWGCWC 3 cut(s) 459, 614, 1274
Alw26I GTCTC 2 cut(s) 136, 822
Alw44I GTGCAC 1 cut(s) 1270
AlwI GGATC 3 cut(s) 433, 526, 1010
AlwNI CAGNNNCTG 1 cut(s) 945
Ama87I CYCGRG 2 cut(s) 110, 1183
AoxI GGCC 9 cut(s) 270, 307, 487, 562, 584, 721, 741, 1167, 1179
ApaLI GTGCAC 1 cut(s) 1270
ApeKI GCWGC 2 cut(s) 579, 1235
Asp718I GGTACC 1 cut(s) 1026
AspLEI GCGC 3 cut(s) 264, 278, 1244
AspS9I GGNCC 1 cut(s) 672
AsuC2I CCSGG 2 cut(s) 179, 205
AsuHPI GGTGA 6 cut(s) 177, 448, 616, 662, 934, 1076
AvaI CYCGRG 2 cut(s) 110, 1183
AvaII GGWCC 1 cut(s) 672
BaeGI GKGCMC 1 cut(s) 1274
BanI GGYRCC 3 cut(s) 780, 1026, 1124
BanII GRGCYC 2 cut(s) 408, 459
BauI CACGAG 1 cut(s) 725
Bbv12I GWGCWC 3 cut(s) 459, 614, 1274
BbvI GCAGC 2 cut(s) 566, 1222
BccI CCATC 4 cut(s) 221, 641, 968, 992
BceAI ACGGC 5 cut(s) 341, 502, 641, 736, 896
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 247, 281
BciT130I CCWGG 1 cut(s) 676
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 2 cut(s) 179, 205
BcoDI GTCTC 2 cut(s) 136, 822
BfaI CTAG 3 cut(s) 342, 366, 1275
BfmI CTRYAG 3 cut(s) 580, 750, 934
BfoI RGCGCY 1 cut(s) 265
BisI GCNGC 5 cut(s) 270, 307, 310, 580, 1236
BlsI GCNGC 5 cut(s) 271, 308, 311, 581, 1237
Bme1390I CCNGG 3 cut(s) 179, 205, 676
Bme18I GGWCC 1 cut(s) 672
BmeRI GACNNNNNGTC 1 cut(s) 658
BmeT110I CYCGRG 2 cut(s) 110, 1183
BmgBI CACGTC 1 cut(s) 682
BmgT120I GGNCC 1 cut(s) 672
BmiI GGNNCC 5 cut(s) 496, 673, 782, 1028, 1126
BmrFI CCNGG 3 cut(s) 179, 205, 676
BmsI GCATC 2 cut(s) 343, 879
Bpu10I CCTNAGC 1 cut(s) 1176
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 2 cut(s) 179, 205
BsaHI GRCGYC 1 cut(s) 654
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 7 cut(s) 111, 126, 178, 490, 574, 600, 675
BsaWI WCCGGW 1 cut(s) 1151
BsaXI ACNNNNNCTCC 8 cut(s) 486, 516, 549, 579, 819, 849, 1249, 1279
Bsc4I CCNNNNNNNGG 4 cut(s) 371, 606, 790, 1009
Bse118I RCCGGY 1 cut(s) 1238
Bse1I ACTGG 2 cut(s) 248, 1091
Bse3DI GCAATG 2 cut(s) 69, 507
BseBI CCWGG 1 cut(s) 676
BseDI CCNNGG 7 cut(s) 111, 126, 178, 490, 574, 600, 675
BseGI GGATG 4 cut(s) 141, 736, 894, 1208
BseLI CCNNNNNNNGG 4 cut(s) 371, 606, 790, 1009
BseMI GCAATG 2 cut(s) 69, 507
BseMII CTCAG 1 cut(s) 1214
BseNI ACTGG 2 cut(s) 248, 1091
BseRI GAGGAG 2 cut(s) 507, 622
BseSI GKGCMC 1 cut(s) 1274
BseX3I CGGCCG 2 cut(s) 307, 487
BseXI GCAGC 2 cut(s) 566, 1222
BseYI CCCAGC 1 cut(s) 945
BsgI GTGCAG 1 cut(s) 72
Bsh1236I CGCG 2 cut(s) 269, 312
Bsh1285I CGRYCG 2 cut(s) 310, 490
BshFI GGCC 9 cut(s) 272, 309, 489, 564, 586, 723, 743, 1169, 1181
BshNI GGYRCC 3 cut(s) 780, 1026, 1124
BsiEI CGRYCG 2 cut(s) 310, 490
BsiHKAI GWGCWC 3 cut(s) 459, 614, 1274
BsiHKCI CYCGRG 2 cut(s) 110, 1183
BsiSI CCGG 5 cut(s) 178, 204, 1152, 1239, 1255
BslFI GGGAC 1 cut(s) 692
BslI CCNNNNNNNGG 4 cut(s) 371, 606, 790, 1009
BsmAI GTCTC 2 cut(s) 136, 822
BsmFI GGGAC 1 cut(s) 692
BsmI GAATGC 2 cut(s) 214, 601
BsnI GGCC 9 cut(s) 272, 309, 489, 564, 586, 723, 743, 1169, 1181
Bso31I GGTCTC 1 cut(s) 136
BsoBI CYCGRG 2 cut(s) 110, 1183
Bsp1286I GDGCHC 4 cut(s) 408, 459, 614, 1274
Bsp143I GATC 4 cut(s) 438, 531, 691, 1015
Bsp19I CCATGG 2 cut(s) 126, 574
BspACI CCGC 5 cut(s) 5, 269, 306, 310, 966
BspANI GGCC 9 cut(s) 272, 309, 489, 564, 586, 723, 743, 1169, 1181
BspCNI CTCAG 1 cut(s) 1213
BspFNI CGCG 2 cut(s) 269, 312
BspLI GGNNCC 5 cut(s) 496, 673, 782, 1028, 1126
BspMAI CTGCAG 2 cut(s) 584, 754
BspPI GGATC 3 cut(s) 433, 526, 1010
BspT107I GGYRCC 3 cut(s) 780, 1026, 1124
BspTNI GGTCTC 1 cut(s) 136
BsrDI GCAATG 2 cut(s) 69, 507
BsrFI RCCGGY 1 cut(s) 1238
BsrI ACTGG 2 cut(s) 248, 1091
BssAI RCCGGY 1 cut(s) 1238
BssECI CCNNGG 7 cut(s) 111, 126, 178, 490, 574, 600, 675
BssMI GATC 4 cut(s) 438, 531, 691, 1015
BssNI GRCGYC 1 cut(s) 654
BssSI CACGAG 1 cut(s) 725
BssT1I CCWWGG 3 cut(s) 126, 574, 600
Bst2BI CACGAG 1 cut(s) 725
Bst2UI CCWGG 1 cut(s) 676
Bst4CI ACNGT 4 cut(s) 224, 618, 850, 1265
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 1 cut(s) 654
BstAPI GCANNNNNTGC 1 cut(s) 889
BstC8I GCNNGC 5 cut(s) 260, 274, 523, 584, 1240
BstDEI CTNAG 2 cut(s) 1176, 1200
BstDSI CCRYGG 2 cut(s) 126, 574
BstEII GGTNACC 2 cut(s) 183, 1045
BstENI CCTNNNNNAGG 1 cut(s) 604
BstF5I GGATG 4 cut(s) 141, 736, 894, 1208
BstFNI CGCG 2 cut(s) 269, 312
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 3 cut(s) 264, 278, 1244
BstKTI GATC 4 cut(s) 441, 534, 694, 1018
BstMAI GTCTC 2 cut(s) 136, 822
BstMBI GATC 4 cut(s) 438, 531, 691, 1015
BstMCI CGRYCG 2 cut(s) 310, 490
BstMWI GCNNNNNNNGC 7 cut(s) 95, 275, 518, 749, 889, 1154, 1241
BstNI CCWGG 1 cut(s) 676
BstNSI RCATGY 1 cut(s) 525
BstPI GGTNACC 2 cut(s) 183, 1045
BstSCI CCNGG 3 cut(s) 177, 203, 674
BstSFI CTRYAG 3 cut(s) 580, 750, 934
BstSLI GKGCMC 1 cut(s) 1274
BstUI CGCG 2 cut(s) 269, 312
BstV1I GCAGC 2 cut(s) 566, 1222
BstXI CCANNNNNNTGG 1 cut(s) 575
BstZI CGGCCG 2 cut(s) 307, 487
BsuRI GGCC 9 cut(s) 272, 309, 489, 564, 586, 723, 743, 1169, 1181
BtgI CCRYGG 2 cut(s) 126, 574
BtgZI GCGATG 2 cut(s) 244, 1203
BtrI CACGTC 1 cut(s) 682
BtsCI GGATG 4 cut(s) 141, 736, 894, 1208
BtsI GCAGTG 1 cut(s) 946
BtsIMutI CAGTG 3 cut(s) 153, 333, 946
Cac8I GCNNGC 5 cut(s) 260, 274, 523, 584, 1240
CaiI CAGNNNCTG 1 cut(s) 945
CciNI GCGGCCGC 1 cut(s) 307
CfoI GCGC 3 cut(s) 264, 278, 1244
Cfr10I RCCGGY 1 cut(s) 1238
Cfr13I GGNCC 1 cut(s) 672
CseI GACGC 1 cut(s) 662
Csp6I GTAC 1 cut(s) 1027
CviQI GTAC 1 cut(s) 1027
DdeI CTNAG 2 cut(s) 1176, 1200
DpnI GATC 4 cut(s) 440, 533, 693, 1017
DpnII GATC 4 cut(s) 438, 531, 691, 1015
DriI GACNNNNNGTC 1 cut(s) 658
EaeI YGGCCR 2 cut(s) 307, 487
EagI CGGCCG 2 cut(s) 307, 487
Eam1104I CTCTTC 1 cut(s) 59
Eam1105I GACNNNNNGTC 1 cut(s) 658
EarI CTCTTC 1 cut(s) 59
EciI GGCGGA 1 cut(s) 981
Ecl136II GAGCTC 1 cut(s) 457
EclXI CGGCCG 2 cut(s) 307, 487
Eco130I CCWWGG 3 cut(s) 126, 574, 600
Eco147I AGGCCT 3 cut(s) 586, 1169, 1181
Eco24I GRGCYC 2 cut(s) 408, 459
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 672
Eco52I CGGCCG 2 cut(s) 307, 487
Eco53kI GAGCTC 1 cut(s) 457
Eco88I CYCGRG 2 cut(s) 110, 1183
Eco91I GGTNACC 2 cut(s) 183, 1045
EcoICRI GAGCTC 1 cut(s) 457
EcoNI CCTNNNNNAGG 1 cut(s) 604
EcoO65I GGTNACC 2 cut(s) 183, 1045
EcoRII CCWGG 1 cut(s) 674
EcoT14I CCWWGG 3 cut(s) 126, 574, 600
EcoT22I ATGCAT 1 cut(s) 527
EcoT38I GRGCYC 2 cut(s) 408, 459
ErhI CCWWGG 3 cut(s) 126, 574, 600
FalI AAGNNNNNCTT 2 cut(s) 1015, 1047
FaqI GGGAC 1 cut(s) 692
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 5 cut(s) 270, 307, 310, 580, 1236
FokI GGATG 4 cut(s) 128, 743, 901, 1195
FriOI GRGCYC 2 cut(s) 408, 459
Fsp4HI GCNGC 5 cut(s) 270, 307, 310, 580, 1236
FspBI CTAG 3 cut(s) 342, 366, 1275
GlaI GCGC 3 cut(s) 263, 277, 1243
GluI GCNGC 5 cut(s) 270, 307, 310, 580, 1236
GsaI CCCAGC 1 cut(s) 949
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 9 cut(s) 272, 309, 489, 564, 586, 723, 743, 1169, 1181
HapII CCGG 5 cut(s) 178, 204, 1152, 1239, 1255
HgaI GACGC 1 cut(s) 662
HhaI GCGC 3 cut(s) 264, 278, 1244
Hin1I GRCGYC 1 cut(s) 654
Hin6I GCGC 3 cut(s) 262, 276, 1242
HinP1I GCGC 3 cut(s) 262, 276, 1242
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 1 cut(s) 1139
HpaII CCGG 5 cut(s) 178, 204, 1152, 1239, 1255
HphI GGTGA 6 cut(s) 177, 448, 616, 662, 934, 1076
Hpy166II GTNNAC 4 cut(s) 361, 505, 925, 1272
Hpy188I TCNGA 6 cut(s) 146, 1020, 1075, 1165, 1203, 1220
Hpy188III TCNNGA 2 cut(s) 763, 1117
Hpy8I GTNNAC 4 cut(s) 361, 505, 925, 1272
Hpy99I CGWCG 3 cut(s) 221, 317, 722
HpyAV CCTTC 2 cut(s) 866, 989
HpyCH4III ACNGT 4 cut(s) 224, 618, 850, 1265
HpyCH4IV ACGT 2 cut(s) 327, 681
HpyCH4V TGCA 8 cut(s) 89, 172, 525, 582, 752, 841, 974, 1272
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 275, 518, 749, 889, 1154, 1241
HpyF3I CTNAG 2 cut(s) 1176, 1200
HpySE526I ACGT 2 cut(s) 327, 681
Hsp92I GRCGYC 1 cut(s) 654
HspAI GCGC 3 cut(s) 262, 276, 1242
KpnI GGTACC 1 cut(s) 1030
KroI GCCGGC 1 cut(s) 1238
KroNI GCCGGC 1 cut(s) 1240
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 4 cut(s) 438, 531, 691, 1015
LmnI GCTCC 6 cut(s) 18, 454, 494, 518, 609, 1154
Lsp1109I GCAGC 2 cut(s) 566, 1222
LweI GCATC 2 cut(s) 343, 879
MaeI CTAG 3 cut(s) 342, 366, 1275
MaeII ACGT 2 cut(s) 327, 681
MalI GATC 4 cut(s) 440, 533, 693, 1017
MboI GATC 4 cut(s) 438, 531, 691, 1015
MboII GAAGA 2 cut(s) 46, 1093
MhlI GDGCHC 4 cut(s) 408, 459, 614, 1274
MluCI AATT 1 cut(s) 1090
MlyI GAGTC 1 cut(s) 1148
MmeI TCCRAC 2 cut(s) 611, 1053
Mph1103I ATGCAT 1 cut(s) 527
MroNI GCCGGC 1 cut(s) 1238
MseI TTAA 1 cut(s) 248
MslI CAYNNNNRTG 3 cut(s) 573, 786, 842
MspI CCGG 5 cut(s) 178, 204, 1152, 1239, 1255
MspR9I CCNGG 3 cut(s) 179, 205, 676
Mva1269I GAATGC 2 cut(s) 214, 601
MvaI CCWGG 1 cut(s) 676
MvnI CGCG 2 cut(s) 269, 312
MwoI GCNNNNNNNGC 7 cut(s) 95, 275, 518, 749, 889, 1154, 1241
NaeI GCCGGC 1 cut(s) 1240
NciI CCSGG 2 cut(s) 179, 205
NcoI CCATGG 2 cut(s) 126, 574
NdeII GATC 4 cut(s) 438, 531, 691, 1015
NgoMIV GCCGGC 1 cut(s) 1238
NlaIV GGNNCC 5 cut(s) 496, 673, 782, 1028, 1126
NmeAIII GCCGAG 1 cut(s) 515
NotI GCGGCCGC 1 cut(s) 307
NsiI ATGCAT 1 cut(s) 527
NspI RCATGY 1 cut(s) 525
PaeI GCATGC 1 cut(s) 525
PaeR7I CTCGAG 1 cut(s) 1183
PceI AGGCCT 3 cut(s) 586, 1169, 1181
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 309
PctI GAATGC 2 cut(s) 214, 601
PdiI GCCGGC 1 cut(s) 1240
PflFI GACNNNGTC 1 cut(s) 833
PkrI GCNGC 5 cut(s) 271, 308, 311, 581, 1237
PleI GAGTC 1 cut(s) 1147
PpsI GAGTC 1 cut(s) 1147
PsiI TTATAA 1 cut(s) 822
Psp124BI GAGCTC 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 674
PspEI GGTNACC 2 cut(s) 183, 1045
PspFI CCCAGC 1 cut(s) 945
PspGI CCWGG 1 cut(s) 674
PspN4I GGNNCC 5 cut(s) 496, 673, 782, 1028, 1126
PspPI GGNCC 1 cut(s) 672
PspXI VCTCGAGB 1 cut(s) 1183
PsrI GAACNNNNNNTAC 2 cut(s) 917, 949
PstI CTGCAG 2 cut(s) 584, 754
PstNI CAGNNNCTG 1 cut(s) 945
PsyI GACNNNGTC 1 cut(s) 833
RsaI GTAC 1 cut(s) 1028
RsaNI GTAC 1 cut(s) 1027
RseI CAYNNNNRTG 3 cut(s) 573, 786, 842
SacI GAGCTC 1 cut(s) 459
SaqAI TTAA 1 cut(s) 248
SatI GCNGC 5 cut(s) 270, 307, 310, 580, 1236
Sau3AI GATC 4 cut(s) 438, 531, 691, 1015
Sau96I GGNCC 1 cut(s) 672
SchI GAGTC 1 cut(s) 1148
ScrFI CCNGG 3 cut(s) 179, 205, 676
SduI GDGCHC 4 cut(s) 408, 459, 614, 1274
SfaNI GCATC 2 cut(s) 343, 879
SfcI CTRYAG 3 cut(s) 580, 750, 934
Sfr274I CTCGAG 1 cut(s) 1183
SinI GGWCC 1 cut(s) 672
SlaI CTCGAG 1 cut(s) 1183
SmiMI CAYNNNNRTG 3 cut(s) 573, 786, 842
SmlI CTYRAG 2 cut(s) 446, 1183
SmoI CTYRAG 2 cut(s) 446, 1183
SphI GCATGC 1 cut(s) 525
Sse9I AATT 1 cut(s) 1090
SseBI AGGCCT 3 cut(s) 586, 1169, 1181
SsiI CCGC 5 cut(s) 5, 269, 306, 310, 966
SspMI CTAG 3 cut(s) 342, 366, 1275
SstI GAGCTC 1 cut(s) 459
StuI AGGCCT 3 cut(s) 586, 1169, 1181
StyD4I CCNGG 3 cut(s) 177, 203, 674
StyI CCWWGG 3 cut(s) 126, 574, 600
TaaI ACNGT 4 cut(s) 224, 618, 850, 1265
TaiI ACGT 2 cut(s) 330, 684
TaqI TCGA 6 cut(s) 12, 102, 216, 662, 764, 1184
TasI AATT 1 cut(s) 1090
TauI GCSGC 3 cut(s) 272, 309, 312
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TscAI CASTG 3 cut(s) 160, 340, 946
TseI GCWGC 2 cut(s) 579, 1235
TspDTI ATGAA 2 cut(s) 58, 479
TspRI CASTG 3 cut(s) 160, 340, 946
Tth111I GACNNNGTC 1 cut(s) 833
VneI GTGCAC 1 cut(s) 1270
VpaK11BI GGWCC 1 cut(s) 672
XagI CCTNNNNNAGG 1 cut(s) 604
XceI RCATGY 1 cut(s) 525
XhoI CTCGAG 1 cut(s) 1183
XspI CTAG 3 cut(s) 342, 366, 1275
Zsp2I ATGCAT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.