Rw3G001980

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010647
Physical Location & Seq
Forward (+)
1764057 .. 1765320
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001980.1

Sequence Viewer

Length: 1218 bp
ATGATTTATATGTGTATATTGGTTCTCTTCCTGTTCGGCTTCATTGCCGAAACACGAATTGCCCCAACAACAGTCGATGACGACCCCGAGGAAGTTGCTTCCATGGTCTCCTTGGGCATCCAAAACAGCATCGAAAAAAAGCTCAGGCAACTCCAGGGATCATCCTCATTATCATGTGCTACCGGGAATTCCATCGACGACTGTTGGCGATGTGACTCCAATTGGTACAACAATCGGAAGCGCCTCGCGGATTGCGTTATTGGTTTCGGCAGGAACACAATTGGAGGCCGCGACGGGCGCTTCTACATCGTCACTGACTCTAGTGACGACGATGCCGTCAACCTTAAAGTCGGGACCCTTCGCCACGCTGTAATACAATCCGAGCCTCTATGGATTGTGTTCCAGCGTGACATGGTGATCCAGCTCAAGCAGGAGCTTATCATGAATAGCTATAAAACCATCGACGGCCGCGGAGCCAATGTTCACATTGCAAATGAAGCATGCATTACAATCCAAAACGTCACGAACATCATAATACATGGCCTCCACATCCATGATTGCAAGCCTACGGGGAATGCCATGGTGAGGAGCTCACCGTCACACTTTGGTTGGAGAGGAATGGCAGACGGTGATGCTGTGTCGATTTTCGGCTCCAGCAACGTGTGGATTGATCACAACTCACTCTCCAACTGCGCCGACGGCCTCGTGGATGTTGTCATGGCCTCAACTGCAGTTACAATCTCTAACAATTACTTCTCGCACCATAATGATGTCATGTTGTTTGGTCACAGCGACTCTTATACTGGAGACAAGGACATGCAAGTGACGGTTGCTTACAACCACTTTGGGGAAGGACTGATGCAAAGGTTGCCGCATTGTAGGCACGGTTATTGTCATGTGGTGAACAATGACTACAGCCACGGTTGGGGGATATACGCCATCGGTGGAGGTGCAAACCCTACCATCAACAGTGAAGGCAACCGATTTGTTGCTGATCTAAAAGTTGGAGTGGAAGAACTGGAATTGGATTTCTCAGGAGATTTGTTCTTGAATGGTGCCTACTTTGTTCCGTCTGGAGCGACCGGAGCTAACTCGGAGGCCTATGCTAAGGCCTCGAGCTATGCAAAAGCTAAGAAATCTTCCATGGTTGGAGCCATAACTTCTGCTGCTGGTGCACTCACCTGCCGCAAGACCATATGTGCACTAGTAACTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

405

Amino Acids

44.03

Weight (kDa)

5.8

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 143 - 375 1.7e-22 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1190
AasI GACNNNNNNGTC 1 cut(s) 335
Acc36I ACCTGC 1 cut(s) 1190
AccB1I GGYRCC 1 cut(s) 1055
AccII CGCG 3 cut(s) 248, 291, 471
AciI CCGC 6 cut(s) 248, 289, 469, 471, 872, 1186
AclWI GGATC 2 cut(s) 166, 412
AcoI YGGCCR 1 cut(s) 466
AcsI RAATTY 1 cut(s) 187
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 3 cut(s) 585, 847, 925
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 1 cut(s) 1051
AhlI ACTAGT 1 cut(s) 1204
AjnI CCWGG 1 cut(s) 153
AloI GAACNNNNNNTCC 2 cut(s) 465, 497
AluBI AGCT 9 cut(s) 142, 424, 436, 450, 591, 1088, 1119, 1130, 1215
AluI AGCT 9 cut(s) 142, 424, 436, 450, 591, 1088, 1119, 1130, 1215
Alw21I GWGCWC 3 cut(s) 593, 1177, 1204
Alw26I GTCTC 2 cut(s) 112, 801
Alw44I GTGCAC 2 cut(s) 1173, 1200
AlwI GGATC 2 cut(s) 166, 412
Ama87I CYCGRG 2 cut(s) 86, 1114
AoxI GGCC 7 cut(s) 286, 466, 541, 700, 720, 1098, 1110
ApaLI GTGCAC 2 cut(s) 1173, 1200
ApeKI GCWGC 1 cut(s) 1166
ApoI RAATTY 1 cut(s) 187
AspLEI GCGC 3 cut(s) 243, 300, 695
AspS9I GGNCC 1 cut(s) 354
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 6 cut(s) 427, 585, 595, 641, 913, 1171
AvaI CYCGRG 2 cut(s) 86, 1114
AvaII GGWCC 1 cut(s) 354
BaeGI GKGCMC 2 cut(s) 1177, 1204
BanI GGYRCC 1 cut(s) 1055
BanII GRGCYC 1 cut(s) 593
BauI CACGAG 1 cut(s) 704
Bbv12I GWGCWC 3 cut(s) 593, 1177, 1204
BbvI GCAGC 1 cut(s) 1153
BccI CCATC 4 cut(s) 200, 467, 947, 971
BceAI ACGGC 3 cut(s) 320, 481, 715
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 1 cut(s) 155
BclI TGATCA 1 cut(s) 670
BcnI CCSGG 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 112, 801
BcuI ACTAGT 1 cut(s) 1204
BfaI CTAG 3 cut(s) 321, 1205, 1212
BfmI CTRYAG 2 cut(s) 729, 913
BfoI RGCGCY 2 cut(s) 244, 301
BfuAI ACCTGC 1 cut(s) 1190
BisI GCNGC 5 cut(s) 289, 469, 872, 1167, 1186
BlsI GCNGC 5 cut(s) 290, 470, 873, 1168, 1187
Bme1390I CCNGG 2 cut(s) 155, 184
Bme18I GGWCC 1 cut(s) 354
BmeT110I CYCGRG 2 cut(s) 86, 1114
BmgT120I GGNCC 1 cut(s) 354
BmiI GGNNCC 6 cut(s) 355, 356, 475, 652, 1057, 1153
BmrFI CCNGG 2 cut(s) 155, 184
BmsI GCATC 5 cut(s) 126, 138, 322, 622, 849
BpmI CTGGAG 4 cut(s) 137, 637, 825, 1095
Bpu10I CCTNAGC 2 cut(s) 143, 1107
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 1 cut(s) 184
BsaI GGTCTC 1 cut(s) 112
BsaJI CCNNGG 8 cut(s) 87, 102, 111, 154, 469, 579, 919, 1143
BsaWI WCCGGW 1 cut(s) 1082
BsaXI ACNNNNNCTCC 6 cut(s) 465, 495, 528, 558, 668, 698
Bsc4I CCNNNNNNNGG 3 cut(s) 585, 847, 925
Bse1I ACTGG 2 cut(s) 808, 1023
Bse3DI GCAATG 2 cut(s) 42, 486
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 8 cut(s) 87, 102, 111, 154, 469, 579, 919, 1143
BseGI GGATG 4 cut(s) 117, 161, 549, 715
BseLI CCNNNNNNNGG 3 cut(s) 585, 847, 925
BseMI GCAATG 2 cut(s) 42, 486
BseMII CTCAG 2 cut(s) 157, 1047
BseNI ACTGG 2 cut(s) 808, 1023
BseRI GAGGAG 1 cut(s) 601
BseSI GKGCMC 2 cut(s) 1177, 1204
BseX3I CGGCCG 1 cut(s) 466
BseXI GCAGC 1 cut(s) 1153
Bsh1236I CGCG 3 cut(s) 248, 291, 471
Bsh1285I CGRYCG 2 cut(s) 469, 1083
BshFI GGCC 7 cut(s) 288, 468, 543, 702, 722, 1100, 1112
BshNI GGYRCC 1 cut(s) 1055
BsiEI CGRYCG 2 cut(s) 469, 1083
BsiHKAI GWGCWC 3 cut(s) 593, 1177, 1204
BsiHKCI CYCGRG 2 cut(s) 86, 1114
BsiSI CCGG 2 cut(s) 183, 1083
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 3 cut(s) 585, 847, 925
BsmAI GTCTC 2 cut(s) 112, 801
BsmFI GGGAC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 7 cut(s) 288, 468, 543, 702, 722, 1100, 1112
Bso31I GGTCTC 1 cut(s) 112
BsoBI CYCGRG 2 cut(s) 86, 1114
Bsp1286I GDGCHC 3 cut(s) 593, 1177, 1204
Bsp143I GATC 4 cut(s) 158, 417, 670, 994
Bsp19I CCATGG 3 cut(s) 102, 579, 1143
BspACI CCGC 6 cut(s) 248, 289, 469, 471, 872, 1186
BspANI GGCC 7 cut(s) 288, 468, 543, 702, 722, 1100, 1112
BspCNI CTCAG 2 cut(s) 156, 1046
BspFNI CGCG 3 cut(s) 248, 291, 471
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 6 cut(s) 355, 356, 475, 652, 1057, 1153
BspMAI CTGCAG 1 cut(s) 733
BspMI ACCTGC 1 cut(s) 1190
BspPI GGATC 2 cut(s) 166, 412
BspT107I GGYRCC 1 cut(s) 1055
BspTNI GGTCTC 1 cut(s) 112
BsrDI GCAATG 2 cut(s) 42, 486
BsrI ACTGG 2 cut(s) 808, 1023
BssECI CCNNGG 8 cut(s) 87, 102, 111, 154, 469, 579, 919, 1143
BssMI GATC 4 cut(s) 158, 417, 670, 994
BssSI CACGAG 1 cut(s) 704
BssT1I CCWWGG 4 cut(s) 102, 111, 579, 1143
Bst2BI CACGAG 1 cut(s) 704
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 8 cut(s) 73, 203, 597, 629, 829, 887, 923, 971
Bst6I CTCTTC 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 502, 563
BstDEI CTNAG 4 cut(s) 143, 1033, 1107, 1131
BstDSI CCRYGG 5 cut(s) 102, 469, 579, 919, 1143
BstF5I GGATG 4 cut(s) 117, 161, 549, 715
BstFNI CGCG 3 cut(s) 248, 291, 471
BstH2I RGCGCY 2 cut(s) 244, 301
BstHHI GCGC 3 cut(s) 243, 300, 695
BstKTI GATC 4 cut(s) 161, 420, 673, 997
BstMAI GTCTC 2 cut(s) 112, 801
BstMBI GATC 4 cut(s) 158, 417, 670, 994
BstMCI CGRYCG 2 cut(s) 469, 1083
BstMWI GCNNNNNNNGC 8 cut(s) 297, 497, 699, 728, 868, 880, 1085, 1172
BstNI CCWGG 1 cut(s) 155
BstNSI RCATGY 2 cut(s) 504, 820
BstSCI CCNGG 2 cut(s) 153, 182
BstSFI CTRYAG 2 cut(s) 729, 913
BstSLI GKGCMC 2 cut(s) 1177, 1204
BstUI CGCG 3 cut(s) 248, 291, 471
BstV1I GCAGC 1 cut(s) 1153
BstZI CGGCCG 1 cut(s) 466
BsuRI GGCC 7 cut(s) 288, 468, 543, 702, 722, 1100, 1112
BtgI CCRYGG 5 cut(s) 102, 469, 579, 919, 1143
BtgZI GCGATG 1 cut(s) 223
BtsCI GGATG 4 cut(s) 117, 161, 549, 715
BtsIMutI CAGTG 2 cut(s) 312, 976
BveI ACCTGC 1 cut(s) 1190
Cac8I GCNNGC 2 cut(s) 502, 563
CciI TCATGA 1 cut(s) 441
CfoI GCGC 3 cut(s) 243, 300, 695
Cfr13I GGNCC 1 cut(s) 354
Cfr42I CCGCGG 1 cut(s) 472
Csp6I GTAC 1 cut(s) 226
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 4 cut(s) 143, 1033, 1107, 1131
DpnI GATC 4 cut(s) 160, 419, 672, 996
DpnII GATC 4 cut(s) 158, 417, 670, 994
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 466
EagI CGGCCG 1 cut(s) 466
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 591
EclXI CGGCCG 1 cut(s) 466
Eco130I CCWWGG 4 cut(s) 102, 111, 579, 1143
Eco147I AGGCCT 2 cut(s) 1100, 1112
Eco24I GRGCYC 1 cut(s) 593
Eco31I GGTCTC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 354
Eco52I CGGCCG 1 cut(s) 466
Eco53kI GAGCTC 1 cut(s) 591
Eco88I CYCGRG 2 cut(s) 86, 1114
EcoICRI GAGCTC 1 cut(s) 591
EcoO109I RGGNCCY 1 cut(s) 354
EcoRI GAATTC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 153
EcoT14I CCWWGG 4 cut(s) 102, 111, 579, 1143
EcoT22I ATGCAT 1 cut(s) 506
EcoT38I GRGCYC 1 cut(s) 593
ErhI CCWWGG 4 cut(s) 102, 111, 579, 1143
FaqI GGGAC 1 cut(s) 367
FauNDI CATATG 1 cut(s) 1196
FbaI TGATCA 1 cut(s) 670
Fnu4HI GCNGC 5 cut(s) 289, 469, 872, 1167, 1186
FokI GGATG 4 cut(s) 104, 148, 536, 722
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 5 cut(s) 289, 469, 872, 1167, 1186
FspBI CTAG 3 cut(s) 321, 1205, 1212
GlaI GCGC 3 cut(s) 242, 299, 694
GluI GCNGC 5 cut(s) 289, 469, 872, 1167, 1186
GsuI CTGGAG 4 cut(s) 137, 637, 825, 1095
HaeII RGCGCY 2 cut(s) 244, 301
HaeIII GGCC 7 cut(s) 288, 468, 543, 702, 722, 1100, 1112
HapII CCGG 2 cut(s) 183, 1083
HhaI GCGC 3 cut(s) 243, 300, 695
Hin6I GCGC 3 cut(s) 241, 298, 693
HinP1I GCGC 3 cut(s) 241, 298, 693
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 3 cut(s) 215, 317, 794
HpaII CCGG 2 cut(s) 183, 1083
HphI GGTGA 6 cut(s) 427, 585, 595, 641, 913, 1171
Hpy166II GTNNAC 5 cut(s) 340, 484, 904, 1175, 1202
Hpy188I TCNGA 3 cut(s) 237, 382, 1096
Hpy188III TCNNGA 6 cut(s) 352, 442, 523, 1035, 1048, 1074
Hpy8I GTNNAC 5 cut(s) 340, 484, 904, 1175, 1202
Hpy99I CGWCG 5 cut(s) 200, 296, 332, 467, 701
HpyAV CCTTC 3 cut(s) 368, 845, 968
HpyCH4III ACNGT 8 cut(s) 73, 203, 597, 629, 829, 887, 923, 971
HpyCH4IV ACGT 2 cut(s) 519, 660
HpyF10VI GCNNNNNNNGC 8 cut(s) 297, 497, 699, 728, 868, 880, 1085, 1172
HpyF3I CTNAG 4 cut(s) 143, 1033, 1107, 1131
HpySE526I ACGT 2 cut(s) 519, 660
HspAI GCGC 3 cut(s) 241, 298, 693
KflI GGGWCCC 1 cut(s) 354
Ksp22I TGATCA 1 cut(s) 670
KspI CCGCGG 1 cut(s) 472
Kzo9I GATC 4 cut(s) 158, 417, 670, 994
LmnI GCTCC 7 cut(s) 433, 473, 588, 656, 1076, 1085, 1151
Lsp1109I GCAGC 1 cut(s) 1153
LweI GCATC 5 cut(s) 126, 138, 322, 622, 849
MaeI CTAG 3 cut(s) 321, 1205, 1212
MaeII ACGT 2 cut(s) 519, 660
MalI GATC 4 cut(s) 160, 419, 672, 996
MboI GATC 4 cut(s) 158, 417, 670, 994
MboII GAAGA 3 cut(s) 19, 1025, 1131
MfeI CAATTG 2 cut(s) 220, 279
MhlI GDGCHC 3 cut(s) 593, 1177, 1204
MluCI AATT 6 cut(s) 57, 187, 220, 279, 748, 1022
MlyI GAGTC 3 cut(s) 209, 311, 788
MmeI TCCRAC 4 cut(s) 590, 711, 985, 1129
Mph1103I ATGCAT 1 cut(s) 506
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 5 cut(s) 172, 552, 765, 768, 821
MspA1I CMGCKG 1 cut(s) 471
MspI CCGG 2 cut(s) 183, 1083
MspR9I CCNGG 2 cut(s) 155, 184
MunI CAATTG 2 cut(s) 220, 279
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 3 cut(s) 248, 291, 471
MwoI GCNNNNNNNGC 8 cut(s) 297, 497, 699, 728, 868, 880, 1085, 1172
NciI CCSGG 1 cut(s) 184
NcoI CCATGG 3 cut(s) 102, 579, 1143
NdeI CATATG 1 cut(s) 1196
NdeII GATC 4 cut(s) 158, 417, 670, 994
NlaIV GGNNCC 6 cut(s) 355, 356, 475, 652, 1057, 1153
NmuCI GTSAC 8 cut(s) 212, 310, 323, 407, 520, 597, 785, 823
NsiI ATGCAT 1 cut(s) 506
NspI RCATGY 2 cut(s) 504, 820
PaeI GCATGC 1 cut(s) 504
PaeR7I CTCGAG 1 cut(s) 1114
PagI TCATGA 1 cut(s) 441
PaqCI CACCTGC 1 cut(s) 1190
PceI AGGCCT 2 cut(s) 1100, 1112
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 333
PctI GAATGC 1 cut(s) 580
PkrI GCNGC 5 cut(s) 290, 470, 873, 1168, 1187
PleI GAGTC 3 cut(s) 209, 311, 788
PpsI GAGTC 3 cut(s) 209, 311, 788
PpuMI RGGWCCY 1 cut(s) 354
Psp124BI GAGCTC 1 cut(s) 593
Psp5II RGGWCCY 1 cut(s) 354
Psp6I CCWGG 1 cut(s) 153
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 6 cut(s) 355, 356, 475, 652, 1057, 1153
PspPI GGNCC 1 cut(s) 354
PspPPI RGGWCCY 1 cut(s) 354
PspXI VCTCGAGB 1 cut(s) 1114
PsrI GAACNNNNNNTAC 2 cut(s) 896, 928
PstI CTGCAG 1 cut(s) 733
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 5 cut(s) 172, 552, 765, 768, 821
SacI GAGCTC 1 cut(s) 593
SacII CCGCGG 1 cut(s) 472
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 5 cut(s) 289, 469, 872, 1167, 1186
Sau3AI GATC 4 cut(s) 158, 417, 670, 994
Sau96I GGNCC 1 cut(s) 354
SchI GAGTC 3 cut(s) 209, 311, 788
ScrFI CCNGG 2 cut(s) 155, 184
SduI GDGCHC 3 cut(s) 593, 1177, 1204
SfaNI GCATC 5 cut(s) 126, 138, 322, 622, 849
SfcI CTRYAG 2 cut(s) 729, 913
Sfr274I CTCGAG 1 cut(s) 1114
Sfr303I CCGCGG 1 cut(s) 472
SgrBI CCGCGG 1 cut(s) 472
SinI GGWCC 1 cut(s) 354
SlaI CTCGAG 1 cut(s) 1114
SmiMI CAYNNNNRTG 5 cut(s) 172, 552, 765, 768, 821
SmlI CTYRAG 2 cut(s) 425, 1114
SmoI CTYRAG 2 cut(s) 425, 1114
SpeI ACTAGT 1 cut(s) 1204
SphI GCATGC 1 cut(s) 504
Sse9I AATT 6 cut(s) 57, 187, 220, 279, 748, 1022
SseBI AGGCCT 2 cut(s) 1100, 1112
SsiI CCGC 6 cut(s) 248, 289, 469, 471, 872, 1186
SspMI CTAG 3 cut(s) 321, 1205, 1212
SstI GAGCTC 1 cut(s) 593
StuI AGGCCT 2 cut(s) 1100, 1112
StyD4I CCNGG 2 cut(s) 153, 182
StyI CCWWGG 4 cut(s) 102, 111, 579, 1143
TaaI ACNGT 8 cut(s) 73, 203, 597, 629, 829, 887, 923, 971
TaiI ACGT 2 cut(s) 522, 663
TaqI TCGA 6 cut(s) 75, 132, 195, 462, 641, 1115
TasI AATT 6 cut(s) 57, 187, 220, 279, 748, 1022
TauI GCSGC 4 cut(s) 291, 471, 874, 1188
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 2 cut(s) 319, 976
TseFI GTSAC 8 cut(s) 212, 310, 323, 407, 520, 597, 785, 823
TseI GCWGC 1 cut(s) 1166
Tsp45I GTSAC 8 cut(s) 212, 310, 323, 407, 520, 597, 785, 823
TspDTI ATGAA 3 cut(s) 31, 458, 510
TspGWI ACGGA 1 cut(s) 1059
TspRI CASTG 2 cut(s) 319, 976
VneI GTGCAC 2 cut(s) 1173, 1200
VpaK11BI GGWCC 1 cut(s) 354
XapI RAATTY 1 cut(s) 187
XceI RCATGY 2 cut(s) 504, 820
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XhoI CTCGAG 1 cut(s) 1114
XspI CTAG 3 cut(s) 321, 1205, 1212
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.