RchiOBHm_Chr5g0000831

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010647
Physical Location & Seq
Forward (+)
457498 .. 458760
1263 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28234

Sequence Viewer

Length: 1263 bp
ATGGTGGTGTCTGAGAGTAATAGATATTGCCTTTATGCATGCATGTGGACGTCGTTGCTTCTAGTGTTTGGTTTCGTTGAAAATTGCTCGAGTTCTGCCGTTGATGACCCAGACCAAATTGGTTCCATGGTGGATATGAGTATCCGCAACAACGGCACTGAAAGAGAAACCATGAGGAAGCAGCAAGCCTCATGTAGAACCGGCAATCCCATCGATGACTGTTGGCGTTGTGATCTCAACTGGTTCAACAACCGAAAACGTCTCGCGGACTGCGCTATTGGTTTTGGCAAAAATGCCAAGGGCGGCCGCGATGGACGCTTTTACGTTGTCTCTGACTCGAGTGACAACGATGCTGTAAACCCTAGACCAGGCACTCTCCGCCACGCTGTCATTCAGATCGAGCCTCTGTGGATCGTCTTCGCCAGGGACATGTTGATCACACTGAAGCAGGAGCTGATCATGAATAGCTTCAAGACCATTGACGGCCGCGGAGTCAACGTTCAAATTGCTTACGGAGCAGGCATAACGATCCAATATGTCACAAACATTATAATCCATGGTGTGCATATCCACCACTGCAAGCCCACTGGCAATGCAATGGTGCGAAGCTCCCCATCTCACTTTGGACGGAGAGGAATGGCTGATGGCGATGCCATCTCCATCTTTGGATCTAAAAACATATGGATCGATCATAATTCGCTCTCCAACTGTTACGATGGCCTTGTAGATGTCGTTGAGGCCTCAACTGCTGTCACCGTCTCTAACAATCTGTTTACGGACCACGACAAGGTGATGCTGTTAGGGCACAATGATGCTTATACTCAAGACAAGGTGATGCAAGTGACCGTTGCTTACAACCGTTTCGGACAGGGACTCGGGCAGAGAATGCCGCATTGTAGGCACGGTAGTTTCCACGTCCTGAACAATGACTACCCTAACGGGTGGGGGGTATATGCCATTGGTGGAGCTGCAAACCCTACCATCAACAGCCAAGGTAACAGATTCTATGCTGACGCAGTTAAGCATCCCTATACGAATCAGGTTACCAAGAGAATTTCGGGAAGCGAGTCAGAGTGGAAGAGATGGAATTGGCGGTCGTCGGGAGATGTTTTCTTGAATGGTGCTTACTTCGTTCCGTCTGGAGATGCTAGTCGGGCAGCCTATGTCAAGGCCTCGAGCACCTTACCCAAGCCAGCTTCCATGGTTGAAGCCATAACGGCTGATGCCGGTGTACTTTCTTGCCGCAGAGACCATGTGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

420

Amino Acids

46.45

Weight (kDa)

8.21

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 151 - 355 8.7e-22 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 551
AatII GACGTC 1 cut(s) 53
AccII CGCG 3 cut(s) 266, 309, 489
AclI AACGTT 1 cut(s) 498
AclWI GGATC 4 cut(s) 419, 523, 676, 692
AcoI YGGCCR 2 cut(s) 304, 484
AcsI RAATTY 1 cut(s) 1053
AcuI CTGAAG 1 cut(s) 464
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 1 cut(s) 1233
AfiI CCNNNNNNNGG 2 cut(s) 368, 787
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 6 cut(s) 80, 247, 472, 503, 1117, 1208
AjiI CACGTC 1 cut(s) 916
AjnI CCWGG 2 cut(s) 367, 422
AloI GAACNNNNNNTCC 4 cut(s) 190, 222, 483, 515
AluBI AGCT 5 cut(s) 454, 468, 609, 968, 1196
AluI AGCT 5 cut(s) 454, 468, 609, 968, 1196
Alw21I GWGCWC 1 cut(s) 1181
Alw26I GTCTC 4 cut(s) 266, 334, 763, 1242
AlwI GGATC 4 cut(s) 419, 523, 676, 692
AlwNI CAGNNNCTG 1 cut(s) 454
Ama87I CYCGRG 4 cut(s) 88, 337, 875, 1174
AoxI GGCC 5 cut(s) 304, 484, 718, 738, 1170
ApeKI GCWGC 3 cut(s) 181, 968, 1157
ApoI RAATTY 1 cut(s) 1053
Asp700I GAANNNNTTC 1 cut(s) 467
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 778
AsuHPI GGTGA 3 cut(s) 745, 802, 844
AvaI CYCGRG 4 cut(s) 88, 337, 875, 1174
AvaII GGWCC 1 cut(s) 778
BaeGI GKGCMC 1 cut(s) 807
BbsI GAAGAC 1 cut(s) 409
Bbv12I GWGCWC 1 cut(s) 1181
BbvI GCAGC 3 cut(s) 193, 955, 1169
BccI CCATC 9 cut(s) 218, 305, 622, 638, 662, 668, 710, 989, 1077
BceAI ACGGC 4 cut(s) 83, 169, 499, 1233
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 2 cut(s) 369, 424
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 2 cut(s) 435, 456
BcoDI GTCTC 4 cut(s) 266, 334, 763, 1242
BfaI CTAG 3 cut(s) 62, 363, 1149
BfuI GTATCC 1 cut(s) 152
BglI GCCNNNNNGGC 1 cut(s) 1217
BisI GCNGC 8 cut(s) 182, 304, 307, 487, 890, 969, 1158, 1243
BlsI GCNGC 8 cut(s) 183, 305, 308, 488, 891, 970, 1159, 1244
Bme1390I CCNGG 2 cut(s) 369, 424
Bme18I GGWCC 1 cut(s) 778
BmeT110I CYCGRG 4 cut(s) 88, 337, 875, 1174
BmgBI CACGTC 1 cut(s) 916
BmgT120I GGNCC 1 cut(s) 778
BmiI GGNNCC 1 cut(s) 124
BmrFI CCNGG 2 cut(s) 369, 424
BmsI GCATC 8 cut(s) 340, 640, 783, 802, 825, 1033, 1135, 1213
BpiI GAAGAC 1 cut(s) 409
BpmI CTGGAG 1 cut(s) 1161
BpuEI CTTGAG 1 cut(s) 807
Bsa29I ATCGAT 2 cut(s) 213, 687
BsaHI GRCGYC 1 cut(s) 50
BsaI GGTCTC 1 cut(s) 1242
BsaJI CCNNGG 7 cut(s) 126, 297, 423, 487, 556, 991, 1200
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 368, 787
Bse118I RCCGGY 2 cut(s) 200, 1226
Bse1I ACTGG 2 cut(s) 245, 592
Bse3DI GCAATG 2 cut(s) 598, 603
BseBI CCWGG 2 cut(s) 369, 424
BseCI ATCGAT 2 cut(s) 213, 687
BseDI CCNNGG 7 cut(s) 126, 297, 423, 487, 556, 991, 1200
BseGI GGATG 1 cut(s) 1024
BseLI CCNNNNNNNGG 2 cut(s) 368, 787
BseMI GCAATG 2 cut(s) 598, 603
BseNI ACTGG 2 cut(s) 245, 592
BseSI GKGCMC 1 cut(s) 807
BseX3I CGGCCG 2 cut(s) 304, 484
BseXI GCAGC 3 cut(s) 193, 955, 1169
Bsh1236I CGCG 3 cut(s) 266, 309, 489
Bsh1285I CGRYCG 3 cut(s) 307, 487, 1097
BshFI GGCC 5 cut(s) 306, 486, 720, 740, 1172
BshVI ATCGAT 2 cut(s) 213, 687
BsiEI CGRYCG 3 cut(s) 307, 487, 1097
BsiHKAI GWGCWC 1 cut(s) 1181
BsiHKCI CYCGRG 4 cut(s) 88, 337, 875, 1174
BsiSI CCGG 2 cut(s) 201, 1227
BslFI GGGAC 2 cut(s) 440, 885
BslI CCNNNNNNNGG 2 cut(s) 368, 787
BsmAI GTCTC 4 cut(s) 266, 334, 763, 1242
BsmBI CGTCTC 2 cut(s) 266, 763
BsmFI GGGAC 2 cut(s) 440, 885
BsmI GAATGC 1 cut(s) 891
BsnI GGCC 5 cut(s) 306, 486, 720, 740, 1172
Bso31I GGTCTC 1 cut(s) 1242
BsoBI CYCGRG 4 cut(s) 88, 337, 875, 1174
Bsp1286I GDGCHC 2 cut(s) 807, 1181
Bsp143I GATC 9 cut(s) 232, 396, 411, 435, 456, 528, 668, 684, 688
Bsp19I CCATGG 3 cut(s) 126, 556, 1200
BspANI GGCC 5 cut(s) 306, 486, 720, 740, 1172
BspCNI CTCAG 1 cut(s) 4
BspDI ATCGAT 2 cut(s) 213, 687
BspFNI CGCG 3 cut(s) 266, 309, 489
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 1 cut(s) 124
BspPI GGATC 4 cut(s) 419, 523, 676, 692
BspTNI GGTCTC 1 cut(s) 1242
BsrDI GCAATG 2 cut(s) 598, 603
BsrFI RCCGGY 2 cut(s) 200, 1226
BsrI ACTGG 2 cut(s) 245, 592
BssAI RCCGGY 2 cut(s) 200, 1226
BssECI CCNNGG 7 cut(s) 126, 297, 423, 487, 556, 991, 1200
BssMI GATC 9 cut(s) 232, 396, 411, 435, 456, 528, 668, 684, 688
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 5 cut(s) 126, 297, 556, 991, 1200
Bst2UI CCWGG 2 cut(s) 369, 424
Bst4CI ACNGT 6 cut(s) 221, 710, 757, 847, 860, 905
Bst6I CTCTTC 1 cut(s) 1073
BstACI GRCGYC 1 cut(s) 50
BstAPI GCANNNNNTGC 1 cut(s) 886
BstC8I GCNNGC 5 cut(s) 40, 186, 520, 581, 1194
BstDEI CTNAG 1 cut(s) 12
BstDSI CCRYGG 4 cut(s) 126, 487, 556, 1200
BstEII GGTNACC 1 cut(s) 1042
BstENI CCTNNNNNAGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 1024
BstFNI CGCG 3 cut(s) 266, 309, 489
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 9 cut(s) 235, 399, 414, 438, 459, 531, 671, 687, 691
BstMAI GTCTC 4 cut(s) 266, 334, 763, 1242
BstMBI GATC 9 cut(s) 232, 396, 411, 435, 456, 528, 668, 684, 688
BstMCI CGRYCG 3 cut(s) 307, 487, 1097
BstNI CCWGG 2 cut(s) 369, 424
BstNSI RCATGY 3 cut(s) 42, 46, 433
BstPI GGTNACC 1 cut(s) 1042
BstSCI CCNGG 2 cut(s) 367, 422
BstSLI GKGCMC 1 cut(s) 807
BstUI CGCG 3 cut(s) 266, 309, 489
BstV1I GCAGC 3 cut(s) 193, 955, 1169
BstV2I GAAGAC 1 cut(s) 409
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
BstZI CGGCCG 2 cut(s) 304, 484
Bsu15I ATCGAT 2 cut(s) 213, 687
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 5 cut(s) 306, 486, 720, 740, 1172
BsuTUI ATCGAT 2 cut(s) 213, 687
BtgI CCRYGG 4 cut(s) 126, 487, 556, 1200
BtgZI GCGATG 2 cut(s) 324, 663
BtrI CACGTC 1 cut(s) 916
BtsCI GGATG 1 cut(s) 1024
BtsI GCAGTG 1 cut(s) 574
BtsIMutI CAGTG 4 cut(s) 156, 440, 574, 585
Cac8I GCNNGC 5 cut(s) 40, 186, 520, 581, 1194
CaiI CAGNNNCTG 1 cut(s) 454
CciI TCATGA 1 cut(s) 459
CciNI GCGGCCGC 1 cut(s) 304
CfoI GCGC 1 cut(s) 275
Cfr10I RCCGGY 2 cut(s) 200, 1226
Cfr13I GGNCC 1 cut(s) 778
Cfr42I CCGCGG 1 cut(s) 490
ClaI ATCGAT 2 cut(s) 213, 687
CseI GACGC 2 cut(s) 324, 1022
Csp6I GTAC 1 cut(s) 1232
CviQI GTAC 1 cut(s) 1232
DdeI CTNAG 1 cut(s) 12
DpnI GATC 9 cut(s) 234, 398, 413, 437, 458, 530, 670, 686, 690
DpnII GATC 9 cut(s) 232, 396, 411, 435, 456, 528, 668, 684, 688
EaeI YGGCCR 2 cut(s) 304, 484
EagI CGGCCG 2 cut(s) 304, 484
Eam1104I CTCTTC 1 cut(s) 1073
EarI CTCTTC 1 cut(s) 1073
EciI GGCGGA 1 cut(s) 368
EclXI CGGCCG 2 cut(s) 304, 484
Eco130I CCWWGG 5 cut(s) 126, 297, 556, 991, 1200
Eco147I AGGCCT 2 cut(s) 740, 1172
Eco31I GGTCTC 1 cut(s) 1242
Eco47I GGWCC 1 cut(s) 778
Eco52I CGGCCG 2 cut(s) 304, 484
Eco57I CTGAAG 1 cut(s) 464
Eco88I CYCGRG 4 cut(s) 88, 337, 875, 1174
Eco91I GGTNACC 1 cut(s) 1042
EcoNI CCTNNNNNAGG 1 cut(s) 366
EcoO65I GGTNACC 1 cut(s) 1042
EcoRII CCWGG 2 cut(s) 367, 422
EcoT14I CCWWGG 5 cut(s) 126, 297, 556, 991, 1200
EcoT22I ATGCAT 2 cut(s) 40, 44
ErhI CCWWGG 5 cut(s) 126, 297, 556, 991, 1200
Esp3I CGTCTC 2 cut(s) 266, 763
FaqI GGGAC 2 cut(s) 440, 885
FauNDI CATATG 1 cut(s) 680
FbaI TGATCA 2 cut(s) 435, 456
Fnu4HI GCNGC 8 cut(s) 182, 304, 307, 487, 890, 969, 1158, 1243
FokI GGATG 1 cut(s) 1011
Fsp4HI GCNGC 8 cut(s) 182, 304, 307, 487, 890, 969, 1158, 1243
FspBI CTAG 3 cut(s) 62, 363, 1149
GlaI GCGC 1 cut(s) 274
GluI GCNGC 8 cut(s) 182, 304, 307, 487, 890, 969, 1158, 1243
GsuI CTGGAG 1 cut(s) 1161
HaeIII GGCC 5 cut(s) 306, 486, 720, 740, 1172
HapII CCGG 2 cut(s) 201, 1227
HgaI GACGC 2 cut(s) 324, 1022
HhaI GCGC 1 cut(s) 275
Hin1I GRCGYC 1 cut(s) 50
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 6 cut(s) 335, 492, 873, 1002, 1036, 1067
HpaII CCGG 2 cut(s) 201, 1227
HphI GGTGA 3 cut(s) 745, 802, 844
Hpy166II GTNNAC 5 cut(s) 48, 358, 496, 774, 1232
Hpy188I TCNGA 5 cut(s) 13, 334, 396, 866, 1072
Hpy188III TCNNGA 8 cut(s) 460, 472, 824, 919, 1059, 1101, 1114, 1140
Hpy8I GTNNAC 5 cut(s) 48, 358, 496, 774, 1232
Hpy99I CGWCG 2 cut(s) 55, 1102
HpyCH4III ACNGT 6 cut(s) 221, 710, 757, 847, 860, 905
HpyCH4IV ACGT 5 cut(s) 50, 259, 324, 498, 915
HpyCH4V TGCA 7 cut(s) 38, 42, 565, 579, 596, 838, 971
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 5 cut(s) 50, 259, 324, 498, 915
Hsp92I GRCGYC 1 cut(s) 50
HspAI GCGC 1 cut(s) 273
Ksp22I TGATCA 2 cut(s) 435, 456
KspI CCGCGG 1 cut(s) 490
Kzo9I GATC 9 cut(s) 232, 396, 411, 435, 456, 528, 668, 684, 688
LmnI GCTCC 4 cut(s) 451, 515, 614, 965
Lsp1109I GCAGC 3 cut(s) 193, 955, 1169
LweI GCATC 8 cut(s) 340, 640, 783, 802, 825, 1033, 1135, 1213
MaeI CTAG 3 cut(s) 62, 363, 1149
MaeII ACGT 5 cut(s) 50, 259, 324, 498, 915
MaeIII GTNAC 7 cut(s) 341, 538, 710, 751, 841, 995, 1042
MalI GATC 9 cut(s) 234, 398, 413, 437, 458, 530, 670, 686, 690
MboI GATC 9 cut(s) 232, 396, 411, 435, 456, 528, 668, 684, 688
MboII GAAGA 2 cut(s) 409, 1090
MflI RGATCY 1 cut(s) 668
MhlI GDGCHC 2 cut(s) 807, 1181
MluCI AATT 6 cut(s) 82, 117, 504, 694, 1053, 1087
MlyI GAGTC 4 cut(s) 329, 501, 867, 1076
MmeI TCCRAC 1 cut(s) 729
MnlI CCTC 7 cut(s) 168, 199, 414, 626, 730, 751, 1183
Mph1103I ATGCAT 2 cut(s) 40, 44
MroXI GAANNNNTTC 1 cut(s) 467
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 2 cut(s) 43, 810
MspA1I CMGCKG 1 cut(s) 489
MspI CCGG 2 cut(s) 201, 1227
MspR9I CCNGG 2 cut(s) 369, 424
Mva1269I GAATGC 1 cut(s) 891
MvaI CCWGG 2 cut(s) 369, 424
MvnI CGCG 3 cut(s) 266, 309, 489
NcoI CCATGG 3 cut(s) 126, 556, 1200
NdeI CATATG 1 cut(s) 680
NdeII GATC 9 cut(s) 232, 396, 411, 435, 456, 528, 668, 684, 688
NlaIV GGNNCC 1 cut(s) 124
NmuCI GTSAC 4 cut(s) 341, 538, 751, 841
NotI GCGGCCGC 1 cut(s) 304
NsiI ATGCAT 2 cut(s) 40, 44
NspI RCATGY 3 cut(s) 42, 46, 433
PaeI GCATGC 1 cut(s) 42
PaeR7I CTCGAG 3 cut(s) 88, 337, 1174
PagI TCATGA 1 cut(s) 459
PceI AGGCCT 2 cut(s) 740, 1172
PciI ACATGT 1 cut(s) 429
PctI GAATGC 1 cut(s) 891
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 1002, 1036
PkrI GCNGC 8 cut(s) 183, 305, 308, 488, 891, 970, 1159, 1244
PleI GAGTC 4 cut(s) 329, 500, 867, 1075
PpsI GAGTC 4 cut(s) 329, 500, 867, 1075
PscI ACATGT 1 cut(s) 429
PsiI TTATAA 1 cut(s) 551
Psp1406I AACGTT 1 cut(s) 498
Psp6I CCWGG 2 cut(s) 367, 422
PspEI GGTNACC 1 cut(s) 1042
PspGI CCWGG 2 cut(s) 367, 422
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 1 cut(s) 778
PspXI VCTCGAGB 3 cut(s) 88, 337, 1174
PsrI GAACNNNNNNTAC 2 cut(s) 914, 946
PstNI CAGNNNCTG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 1 cut(s) 1233
RsaNI GTAC 1 cut(s) 1232
RseI CAYNNNNRTG 2 cut(s) 43, 810
SacII CCGCGG 1 cut(s) 490
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 8 cut(s) 182, 304, 307, 487, 890, 969, 1158, 1243
Sau3AI GATC 9 cut(s) 232, 396, 411, 435, 456, 528, 668, 684, 688
Sau96I GGNCC 1 cut(s) 778
SchI GAGTC 4 cut(s) 329, 501, 867, 1076
ScrFI CCNGG 2 cut(s) 369, 424
SduI GDGCHC 2 cut(s) 807, 1181
SfaNI GCATC 8 cut(s) 340, 640, 783, 802, 825, 1033, 1135, 1213
Sfr274I CTCGAG 3 cut(s) 88, 337, 1174
Sfr303I CCGCGG 1 cut(s) 490
SgrBI CCGCGG 1 cut(s) 490
SinI GGWCC 1 cut(s) 778
SlaI CTCGAG 3 cut(s) 88, 337, 1174
SmiMI CAYNNNNRTG 2 cut(s) 43, 810
SmlI CTYRAG 4 cut(s) 88, 337, 822, 1174
SmoI CTYRAG 4 cut(s) 88, 337, 822, 1174
SphI GCATGC 1 cut(s) 42
Sse9I AATT 6 cut(s) 82, 117, 504, 694, 1053, 1087
SseBI AGGCCT 2 cut(s) 740, 1172
SspMI CTAG 3 cut(s) 62, 363, 1149
StuI AGGCCT 2 cut(s) 740, 1172
StyD4I CCNGG 2 cut(s) 367, 422
StyI CCWWGG 5 cut(s) 126, 297, 556, 991, 1200
TaaI ACNGT 6 cut(s) 221, 710, 757, 847, 860, 905
TaiI ACGT 5 cut(s) 53, 262, 327, 501, 918
TaqI TCGA 6 cut(s) 89, 213, 338, 399, 687, 1175
TasI AATT 6 cut(s) 82, 117, 504, 694, 1053, 1087
TatI WGTACW 1 cut(s) 1231
TauI GCSGC 5 cut(s) 306, 309, 489, 892, 1245
TfiI GAWTC 2 cut(s) 1002, 1036
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 4 cut(s) 163, 447, 581, 592
TseFI GTSAC 4 cut(s) 341, 538, 751, 841
TseI GCWGC 3 cut(s) 181, 968, 1157
Tsp45I GTSAC 4 cut(s) 341, 538, 751, 841
TspDTI ATGAA 1 cut(s) 476
TspGWI ACGGA 4 cut(s) 528, 643, 791, 1125
TspRI CASTG 4 cut(s) 163, 447, 581, 592
VpaK11BI GGWCC 1 cut(s) 778
XagI CCTNNNNNAGG 1 cut(s) 366
XapI RAATTY 1 cut(s) 1053
XceI RCATGY 3 cut(s) 42, 46, 433
XhoI CTCGAG 3 cut(s) 88, 337, 1174
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 3 cut(s) 62, 363, 1149
ZraI GACGTC 1 cut(s) 51
Zsp2I ATGCAT 2 cut(s) 40, 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.