Rroxscaffold_6G00427880

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010647
Physical Location & Seq
Forward (+)
48443157 .. 48444445
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00427880.1

Sequence Viewer

Length: 1278 bp
ATGGCAGTGTTTGAGTGCATTAAGAGAATGATTTATATGTGCATATTGGTTCTCTTCCTGTTCGGCTTCATTGCCGAAACACGAACTGCCCCAACAACAGTCGATGACGACCCCGAGGAAGTTGCTTCCATGCATCCAAAACAGCGTAAAAAAAAGCTCAGGCAACTCCAGGGATCATCCTCATTATCATGTGCTACTGGGAATTCCATCGACGACTGTTGGCGATGTGACTCCAATTGGTACAACAATCGGAAGCGCCTCGTGGACTGCGCTATTGGTTTCGGCAGGAACACAATTGGAGGCCGCGACGGGCGCTTCTACATCGTCACTGACTCTAGTGACGACGATGCTGTCAACCTTAAAGCCGGGACCCTTCGCCACGCTGCAATACAATCCGAGCCTCTATGGATTGTGTTCCAGCATGACATGGTGATCCAGCTCAAGCAGGAGCTTATCATGAATAGCTATAAAACCATCGACGGCCGCGGAGCCAATGTTCACATTGCAAATGAAGCAGGCATTACAATCCAAAACGTCACGAACATCATAATACATGGCCTCCACATCCATGACTGCAAGCCTACGGGGAATGCCATGGTGAGGAGCTCACCGTCACACTTTGGTTGGAGAGGAATGGCAGACGGTGATGCTGTGTCGATTTTCGGCTCCAGCAACGTGTGGATTGATCACAACTCACTCTCCAACTGCGCCGACGGCCTCGTGGATGTTGTCATGGCCTCAACTGCAGTTACAATCTCTAACAATTACTTCTCGCACCATAATGATGTCATGTTGTTTGGTCACAGCGACTCTTATACTGGAGACAAGGACATGCAAGTGACGGTTGCTTACAACCACTTTGGGGAAGGACTGATGCAAAGGTTGCCGCATTGTAGGCACGGTTATTGTCATGTGGTGAACAATGACTACAGCCACGGTTGGAGGATATACGCCATTGGTGGAGGTGCAAACCCTACCATCAACAGCGAAGGCAACCGATTTGTTGCTGATCTGAAAAGGTACCCGGATTCGAGTGAGGTTACCAAGAGGACTTCGGAGGACAAGTTGGAGTGGAAGAACTGGAATTGGATTTCTCAGGGAGATTTGTTCTTGAATGGTGCCTACTTTGTTCCGTCTGGAGAAACCGGAGCTAACTCAGAGGCCTATGCTAAGGCCTCGAGCTATGCAAAAGCTAAGAAATCTTCCATGGTTGGAACCATAACTTCTGCTGCCGGTGCACTCACCTGCCGCAAAGACCATATGTGCACTAGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

425

Amino Acids

47.05

Weight (kDa)

6.64

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 148 - 353 1.9e-22 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1253
AasI GACNNNNNNGTC 1 cut(s) 350
Acc36I ACCTGC 1 cut(s) 1253
Acc65I GGTACC 1 cut(s) 1020
AccB1I GGYRCC 2 cut(s) 1020, 1118
AccII CGCG 2 cut(s) 306, 486
AciI CCGC 5 cut(s) 304, 484, 486, 887, 1249
AclWI GGATC 2 cut(s) 181, 427
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 1 cut(s) 202
AfaI GTAC 2 cut(s) 242, 1022
AfiI CCNNNNNNNGG 2 cut(s) 600, 862
AflIII ACRYGT 1 cut(s) 675
AgsI TTSAA 1 cut(s) 1114
AhlI ACTAGT 1 cut(s) 1268
AjnI CCWGG 1 cut(s) 168
AloI GAACNNNNNNTCC 2 cut(s) 480, 512
AluBI AGCT 8 cut(s) 157, 439, 451, 465, 606, 1151, 1182, 1193
AluI AGCT 8 cut(s) 157, 439, 451, 465, 606, 1151, 1182, 1193
Alw21I GWGCWC 3 cut(s) 608, 1240, 1268
Alw26I GTCTC 1 cut(s) 816
Alw44I GTGCAC 2 cut(s) 1236, 1264
AlwI GGATC 2 cut(s) 181, 427
Ama87I CYCGRG 2 cut(s) 113, 1177
AoxI GGCC 7 cut(s) 301, 481, 556, 715, 735, 1161, 1173
ApaLI GTGCAC 2 cut(s) 1236, 1264
ApeKI GCWGC 2 cut(s) 383, 1229
ApoI RAATTY 1 cut(s) 202
Asp718I GGTACC 1 cut(s) 1020
AspLEI GCGC 4 cut(s) 258, 272, 315, 710
AspS9I GGNCC 1 cut(s) 369
AsuC2I CCSGG 2 cut(s) 367, 1025
AsuHPI GGTGA 6 cut(s) 442, 600, 610, 656, 928, 1234
AvaI CYCGRG 2 cut(s) 113, 1177
AvaII GGWCC 1 cut(s) 369
BaeGI GKGCMC 2 cut(s) 1240, 1268
BanI GGYRCC 2 cut(s) 1020, 1118
BanII GRGCYC 1 cut(s) 608
BauI CACGAG 2 cut(s) 260, 719
Bbv12I GWGCWC 3 cut(s) 608, 1240, 1268
BbvI GCAGC 2 cut(s) 370, 1216
BccI CCATC 3 cut(s) 215, 482, 986
BceAI ACGGC 2 cut(s) 496, 730
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 2 cut(s) 367, 1025
BcoDI GTCTC 1 cut(s) 816
BcuI ACTAGT 1 cut(s) 1268
BfaI CTAG 3 cut(s) 336, 1269, 1276
BfmI CTRYAG 2 cut(s) 744, 928
BfoI RGCGCY 2 cut(s) 259, 316
BfuAI ACCTGC 1 cut(s) 1253
BisI GCNGC 6 cut(s) 304, 384, 484, 887, 1230, 1249
BlsI GCNGC 6 cut(s) 305, 385, 485, 888, 1231, 1250
Bme1390I CCNGG 3 cut(s) 170, 367, 1025
Bme18I GGWCC 1 cut(s) 369
BmeT110I CYCGRG 2 cut(s) 113, 1177
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 7 cut(s) 370, 371, 490, 667, 1022, 1120, 1216
BmrFI CCNGG 3 cut(s) 170, 367, 1025
BmrI ACTGGG 1 cut(s) 207
BmsI GCATC 4 cut(s) 142, 337, 637, 864
BmuI ACTGGG 1 cut(s) 207
BpmI CTGGAG 4 cut(s) 152, 652, 840, 1158
Bpu10I CCTNAGC 2 cut(s) 158, 1170
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 2 cut(s) 367, 1025
BsaJI CCNNGG 6 cut(s) 114, 169, 484, 594, 934, 1206
BsaWI WCCGGW 1 cut(s) 1145
BsaXI ACNNNNNCTCC 8 cut(s) 480, 510, 543, 573, 683, 713, 1049, 1079
Bsc4I CCNNNNNNNGG 2 cut(s) 600, 862
Bse118I RCCGGY 1 cut(s) 1232
Bse1I ACTGG 3 cut(s) 202, 823, 1085
Bse3DI GCAATG 2 cut(s) 69, 501
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 6 cut(s) 114, 169, 484, 594, 934, 1206
BseGI GGATG 4 cut(s) 133, 176, 564, 730
BseLI CCNNNNNNNGG 2 cut(s) 600, 862
BseMI GCAATG 2 cut(s) 69, 501
BseMII CTCAG 3 cut(s) 172, 1109, 1170
BseNI ACTGG 3 cut(s) 202, 823, 1085
BseRI GAGGAG 1 cut(s) 616
BseSI GKGCMC 2 cut(s) 1240, 1268
BseX3I CGGCCG 1 cut(s) 481
BseXI GCAGC 2 cut(s) 370, 1216
Bsh1236I CGCG 2 cut(s) 306, 486
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 7 cut(s) 303, 483, 558, 717, 737, 1163, 1175
BshNI GGYRCC 2 cut(s) 1020, 1118
BsiEI CGRYCG 1 cut(s) 484
BsiHKAI GWGCWC 3 cut(s) 608, 1240, 1268
BsiHKCI CYCGRG 2 cut(s) 113, 1177
BsiSI CCGG 4 cut(s) 366, 1025, 1146, 1233
BslFI GGGAC 1 cut(s) 382
BslI CCNNNNNNNGG 2 cut(s) 600, 862
BsmAI GTCTC 1 cut(s) 816
BsmFI GGGAC 1 cut(s) 382
BsmI GAATGC 1 cut(s) 595
BsnI GGCC 7 cut(s) 303, 483, 558, 717, 737, 1163, 1175
BsoBI CYCGRG 2 cut(s) 113, 1177
Bsp1286I GDGCHC 3 cut(s) 608, 1240, 1268
Bsp143I GATC 4 cut(s) 173, 432, 685, 1009
Bsp19I CCATGG 2 cut(s) 594, 1206
BspACI CCGC 5 cut(s) 304, 484, 486, 887, 1249
BspANI GGCC 7 cut(s) 303, 483, 558, 717, 737, 1163, 1175
BspCNI CTCAG 3 cut(s) 171, 1108, 1169
BspFNI CGCG 2 cut(s) 306, 486
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 7 cut(s) 370, 371, 490, 667, 1022, 1120, 1216
BspMAI CTGCAG 1 cut(s) 748
BspMI ACCTGC 1 cut(s) 1253
BspPI GGATC 2 cut(s) 181, 427
BspT107I GGYRCC 2 cut(s) 1020, 1118
BsrDI GCAATG 2 cut(s) 69, 501
BsrFI RCCGGY 1 cut(s) 1232
BsrI ACTGG 3 cut(s) 202, 823, 1085
BssAI RCCGGY 1 cut(s) 1232
BssECI CCNNGG 6 cut(s) 114, 169, 484, 594, 934, 1206
BssMI GATC 4 cut(s) 173, 432, 685, 1009
BssSI CACGAG 2 cut(s) 260, 719
BssT1I CCWWGG 2 cut(s) 594, 1206
Bst2BI CACGAG 2 cut(s) 260, 719
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 7 cut(s) 100, 218, 612, 644, 844, 902, 938
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 883
BstC8I GCNNGC 2 cut(s) 517, 578
BstDEI CTNAG 5 cut(s) 158, 1095, 1156, 1170, 1194
BstDSI CCRYGG 4 cut(s) 484, 594, 934, 1206
BstEII GGTNACC 1 cut(s) 1039
BstF5I GGATG 4 cut(s) 133, 176, 564, 730
BstFNI CGCG 2 cut(s) 306, 486
BstH2I RGCGCY 2 cut(s) 259, 316
BstHHI GCGC 4 cut(s) 258, 272, 315, 710
BstKTI GATC 4 cut(s) 176, 435, 688, 1012
BstMAI GTCTC 1 cut(s) 816
BstMBI GATC 4 cut(s) 173, 432, 685, 1009
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 7 cut(s) 312, 512, 714, 743, 883, 895, 1235
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 835
BstPI GGTNACC 1 cut(s) 1039
BstSCI CCNGG 3 cut(s) 168, 365, 1023
BstSFI CTRYAG 2 cut(s) 744, 928
BstSLI GKGCMC 2 cut(s) 1240, 1268
BstUI CGCG 2 cut(s) 306, 486
BstV1I GCAGC 2 cut(s) 370, 1216
BstZI CGGCCG 1 cut(s) 481
BsuRI GGCC 7 cut(s) 303, 483, 558, 717, 737, 1163, 1175
BtgI CCRYGG 4 cut(s) 484, 594, 934, 1206
BtgZI GCGATG 1 cut(s) 238
BtsCI GGATG 4 cut(s) 133, 176, 564, 730
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 2 cut(s) 12, 327
BveI ACCTGC 1 cut(s) 1253
Cac8I GCNNGC 2 cut(s) 517, 578
CciI TCATGA 1 cut(s) 456
CfoI GCGC 4 cut(s) 258, 272, 315, 710
Cfr10I RCCGGY 1 cut(s) 1232
Cfr13I GGNCC 1 cut(s) 369
Cfr42I CCGCGG 1 cut(s) 487
Csp6I GTAC 2 cut(s) 241, 1021
CviQI GTAC 2 cut(s) 241, 1021
DdeI CTNAG 5 cut(s) 158, 1095, 1156, 1170, 1194
DpnI GATC 4 cut(s) 175, 434, 687, 1011
DpnII GATC 4 cut(s) 173, 432, 685, 1009
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 1 cut(s) 481
EagI CGGCCG 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 606
EclXI CGGCCG 1 cut(s) 481
Eco130I CCWWGG 2 cut(s) 594, 1206
Eco147I AGGCCT 2 cut(s) 1163, 1175
Eco24I GRGCYC 1 cut(s) 608
Eco47I GGWCC 1 cut(s) 369
Eco52I CGGCCG 1 cut(s) 481
Eco53kI GAGCTC 1 cut(s) 606
Eco88I CYCGRG 2 cut(s) 113, 1177
Eco91I GGTNACC 1 cut(s) 1039
EcoICRI GAGCTC 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 369
EcoO65I GGTNACC 1 cut(s) 1039
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 2 cut(s) 594, 1206
EcoT22I ATGCAT 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 608
ErhI CCWWGG 2 cut(s) 594, 1206
FaqI GGGAC 1 cut(s) 382
FauNDI CATATG 1 cut(s) 1260
FbaI TGATCA 1 cut(s) 685
Fnu4HI GCNGC 6 cut(s) 304, 384, 484, 887, 1230, 1249
FokI GGATG 4 cut(s) 120, 163, 551, 737
FriOI GRGCYC 1 cut(s) 608
Fsp4HI GCNGC 6 cut(s) 304, 384, 484, 887, 1230, 1249
FspBI CTAG 3 cut(s) 336, 1269, 1276
GlaI GCGC 4 cut(s) 257, 271, 314, 709
GluI GCNGC 6 cut(s) 304, 384, 484, 887, 1230, 1249
GsuI CTGGAG 4 cut(s) 152, 652, 840, 1158
HaeII RGCGCY 2 cut(s) 259, 316
HaeIII GGCC 7 cut(s) 303, 483, 558, 717, 737, 1163, 1175
HapII CCGG 4 cut(s) 366, 1025, 1146, 1233
HhaI GCGC 4 cut(s) 258, 272, 315, 710
Hin6I GCGC 4 cut(s) 256, 270, 313, 708
HinP1I GCGC 4 cut(s) 256, 270, 313, 708
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 4 cut(s) 230, 332, 809, 1028
HpaII CCGG 4 cut(s) 366, 1025, 1146, 1233
HphI GGTGA 6 cut(s) 442, 600, 610, 656, 928, 1234
Hpy166II GTNNAC 6 cut(s) 265, 355, 499, 919, 1238, 1266
Hpy188I TCNGA 5 cut(s) 252, 397, 1014, 1057, 1159
Hpy188III TCNNGA 4 cut(s) 457, 538, 1111, 1137
Hpy8I GTNNAC 6 cut(s) 265, 355, 499, 919, 1238, 1266
Hpy99I CGWCG 5 cut(s) 215, 311, 347, 482, 716
HpyAV CCTTC 3 cut(s) 383, 860, 983
HpyCH4III ACNGT 7 cut(s) 100, 218, 612, 644, 844, 902, 938
HpyCH4IV ACGT 2 cut(s) 534, 675
HpyF10VI GCNNNNNNNGC 7 cut(s) 312, 512, 714, 743, 883, 895, 1235
HpyF3I CTNAG 5 cut(s) 158, 1095, 1156, 1170, 1194
HpySE526I ACGT 2 cut(s) 534, 675
HspAI GCGC 4 cut(s) 256, 270, 313, 708
KflI GGGWCCC 1 cut(s) 369
KpnI GGTACC 1 cut(s) 1024
Ksp22I TGATCA 1 cut(s) 685
KspI CCGCGG 1 cut(s) 487
Kzo9I GATC 4 cut(s) 173, 432, 685, 1009
LmnI GCTCC 5 cut(s) 448, 488, 603, 671, 1148
Lsp1109I GCAGC 2 cut(s) 370, 1216
LweI GCATC 4 cut(s) 142, 337, 637, 864
MaeI CTAG 3 cut(s) 336, 1269, 1276
MaeII ACGT 2 cut(s) 534, 675
MalI GATC 4 cut(s) 175, 434, 687, 1011
MboI GATC 4 cut(s) 173, 432, 685, 1009
MboII GAAGA 3 cut(s) 46, 1087, 1194
MfeI CAATTG 2 cut(s) 235, 294
MhlI GDGCHC 3 cut(s) 608, 1240, 1268
MluCI AATT 5 cut(s) 202, 235, 294, 763, 1084
MlyI GAGTC 3 cut(s) 224, 326, 803
MmeI TCCRAC 5 cut(s) 605, 726, 920, 1047, 1192
Mph1103I ATGCAT 1 cut(s) 135
MseI TTAA 2 cut(s) 21, 360
MslI CAYNNNNRTG 5 cut(s) 187, 567, 780, 783, 836
MspA1I CMGCKG 1 cut(s) 486
MspI CCGG 4 cut(s) 366, 1025, 1146, 1233
MspR9I CCNGG 3 cut(s) 170, 367, 1025
MunI CAATTG 2 cut(s) 235, 294
Mva1269I GAATGC 1 cut(s) 595
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 306, 486
MwoI GCNNNNNNNGC 7 cut(s) 312, 512, 714, 743, 883, 895, 1235
NciI CCSGG 2 cut(s) 367, 1025
NcoI CCATGG 2 cut(s) 594, 1206
NdeI CATATG 1 cut(s) 1260
NdeII GATC 4 cut(s) 173, 432, 685, 1009
NlaIV GGNNCC 7 cut(s) 370, 371, 490, 667, 1022, 1120, 1216
NmuCI GTSAC 7 cut(s) 227, 325, 338, 535, 612, 800, 838
NsiI ATGCAT 1 cut(s) 135
NspI RCATGY 1 cut(s) 835
PaeR7I CTCGAG 1 cut(s) 1177
PagI TCATGA 1 cut(s) 456
PaqCI CACCTGC 1 cut(s) 1253
PceI AGGCCT 2 cut(s) 1163, 1175
PctI GAATGC 1 cut(s) 595
PfeI GAWTC 1 cut(s) 1028
PkrI GCNGC 6 cut(s) 305, 385, 485, 888, 1231, 1250
PleI GAGTC 3 cut(s) 224, 326, 803
PpsI GAGTC 3 cut(s) 224, 326, 803
PpuMI RGGWCCY 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 608
Psp5II RGGWCCY 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 168
PspEI GGTNACC 1 cut(s) 1039
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 7 cut(s) 370, 371, 490, 667, 1022, 1120, 1216
PspPI GGNCC 1 cut(s) 369
PspPPI RGGWCCY 1 cut(s) 369
PspXI VCTCGAGB 1 cut(s) 1177
PsrI GAACNNNNNNTAC 2 cut(s) 911, 943
PstI CTGCAG 1 cut(s) 748
RsaI GTAC 2 cut(s) 242, 1022
RsaNI GTAC 2 cut(s) 241, 1021
RseI CAYNNNNRTG 5 cut(s) 187, 567, 780, 783, 836
SacI GAGCTC 1 cut(s) 608
SacII CCGCGG 1 cut(s) 487
SaqAI TTAA 2 cut(s) 21, 360
SatI GCNGC 6 cut(s) 304, 384, 484, 887, 1230, 1249
Sau3AI GATC 4 cut(s) 173, 432, 685, 1009
Sau96I GGNCC 1 cut(s) 369
SchI GAGTC 3 cut(s) 224, 326, 803
ScrFI CCNGG 3 cut(s) 170, 367, 1025
SduI GDGCHC 3 cut(s) 608, 1240, 1268
SfaNI GCATC 4 cut(s) 142, 337, 637, 864
SfcI CTRYAG 2 cut(s) 744, 928
Sfr274I CTCGAG 1 cut(s) 1177
Sfr303I CCGCGG 1 cut(s) 487
SgrBI CCGCGG 1 cut(s) 487
SinI GGWCC 1 cut(s) 369
SlaI CTCGAG 1 cut(s) 1177
SmiMI CAYNNNNRTG 5 cut(s) 187, 567, 780, 783, 836
SmlI CTYRAG 2 cut(s) 440, 1177
SmoI CTYRAG 2 cut(s) 440, 1177
SpeI ACTAGT 1 cut(s) 1268
Sse9I AATT 5 cut(s) 202, 235, 294, 763, 1084
SseBI AGGCCT 2 cut(s) 1163, 1175
SsiI CCGC 5 cut(s) 304, 484, 486, 887, 1249
SspMI CTAG 3 cut(s) 336, 1269, 1276
SstI GAGCTC 1 cut(s) 608
StuI AGGCCT 2 cut(s) 1163, 1175
StyD4I CCNGG 3 cut(s) 168, 365, 1023
StyI CCWWGG 2 cut(s) 594, 1206
TaaI ACNGT 7 cut(s) 100, 218, 612, 644, 844, 902, 938
TaiI ACGT 2 cut(s) 537, 678
TaqI TCGA 6 cut(s) 102, 210, 477, 656, 1031, 1178
TasI AATT 5 cut(s) 202, 235, 294, 763, 1084
TauI GCSGC 4 cut(s) 306, 486, 889, 1251
TfiI GAWTC 1 cut(s) 1028
Tru1I TTAA 2 cut(s) 21, 360
Tru9I TTAA 2 cut(s) 21, 360
TscAI CASTG 2 cut(s) 12, 334
TseFI GTSAC 7 cut(s) 227, 325, 338, 535, 612, 800, 838
TseI GCWGC 2 cut(s) 383, 1229
Tsp45I GTSAC 7 cut(s) 227, 325, 338, 535, 612, 800, 838
TspDTI ATGAA 3 cut(s) 58, 473, 525
TspGWI ACGGA 1 cut(s) 1122
TspRI CASTG 2 cut(s) 12, 334
VneI GTGCAC 2 cut(s) 1236, 1264
VpaK11BI GGWCC 1 cut(s) 369
XapI RAATTY 1 cut(s) 202
XceI RCATGY 1 cut(s) 835
XhoI CTCGAG 1 cut(s) 1177
XspI CTAG 3 cut(s) 336, 1269, 1276
Zsp2I ATGCAT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.