Rmu_co8353889.1_g000001

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8353889.1
OG0010647
Physical Location & Seq
Forward (+)
29 .. 1053
1025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8353889.1_g000001.1.cds

Sequence Viewer

Length: 1025 bp
atggcagtgtttgagtgcattaagagaatgatttatatgtgtatattggttctcttcctgttcggcttcattgccgaaacacgaattgccccaacaacagtcgatgacgaccccgaggaagttgcttccatggtctccttgagcatccaaaacagcatcgaaaaaaagctcaggcaactccagggatcatcctcattatcatgtgctaccgggaattccatcgacgactgttggcgatgtgactccaattggtacaacaatcggaagcgcctcgcggattgcgctattggtttcggcaggaacacaattggaggccgcgacgggcgcttctacatcgtcactgactctagtgacgacgatgccgtcaaccttaaagtcgggacccttcgccacgctgtaatacaatccgagcctctatggattgtgttccagcgtgacatggtgatccagctcaagcaggagcttatcatgaatagctataaaaccatcgacggccgcggagccaatgttcacattgcaaatgaagcatgcattacaatccaaaacgtcacgaacatcataatacatggcctccacatccatgattgcaagcctacggggaatgccatggtgaggagctcaccgtcacactttggttggagaggaatggcagacggtgatgctgtgtcgattttcggctccagccacgtgtggattgatcacaactcactctccaactgcgccgacggcctcgtggatgttgtcatggcctcaactgcagttacaatctctaacaattacttctcgcaccataatgatgtcatgttgtttggtcacagcgactcttatactggagacaaggacatgcaagtgacggttgcttacaaccactttggggaaggactgatgcaaaggttgccgcattgtaggcacggttattgtcatgtggtgaacaatgactacagccacggttgggggatatacgccatcggtggaggtgcaaaccctaccatcaacagtgaaggcaaccgatttgttgctgatct
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

342

Amino Acids

37.86

Weight (kDa)

5.84

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 362
AccII CGCG 3 cut(s) 275, 318, 498
AciI CCGC 5 cut(s) 275, 316, 496, 498, 899
AclWI GGATC 2 cut(s) 193, 439
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 1 cut(s) 214
AcvI CACGTG 1 cut(s) 688
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 3 cut(s) 612, 874, 952
AflIII ACRYGT 1 cut(s) 687
AjnI CCWGG 1 cut(s) 180
AloI GAACNNNNNNTCC 2 cut(s) 492, 524
AluBI AGCT 5 cut(s) 169, 451, 463, 477, 618
AluI AGCT 5 cut(s) 169, 451, 463, 477, 618
Alw21I GWGCWC 1 cut(s) 620
Alw26I GTCTC 2 cut(s) 139, 828
AlwI GGATC 2 cut(s) 193, 439
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 5 cut(s) 313, 493, 568, 727, 747
ApoI RAATTY 1 cut(s) 214
AspLEI GCGC 4 cut(s) 270, 284, 327, 722
AspS9I GGNCC 1 cut(s) 381
AsuC2I CCSGG 1 cut(s) 211
AsuHPI GGTGA 5 cut(s) 454, 612, 622, 668, 940
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 1 cut(s) 381
BanII GRGCYC 1 cut(s) 620
BauI CACGAG 1 cut(s) 731
BbrPI CACGTG 1 cut(s) 688
Bbv12I GWGCWC 1 cut(s) 620
BccI CCATC 4 cut(s) 227, 494, 974, 998
BceAI ACGGC 3 cut(s) 347, 508, 742
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 1 cut(s) 182
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 2 cut(s) 139, 828
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 2 cut(s) 756, 940
BfoI RGCGCY 2 cut(s) 271, 328
BisI GCNGC 3 cut(s) 316, 496, 899
BlsI GCNGC 3 cut(s) 317, 497, 900
Bme1390I CCNGG 2 cut(s) 182, 211
Bme18I GGWCC 1 cut(s) 381
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 381
BmiI GGNNCC 4 cut(s) 382, 383, 502, 679
BmrFI CCNGG 2 cut(s) 182, 211
BmsI GCATC 5 cut(s) 153, 165, 349, 649, 876
BpmI CTGGAG 3 cut(s) 164, 664, 852
Bpu10I CCTNAGC 1 cut(s) 170
BpuEI CTTGAG 2 cut(s) 160, 437
BpuMI CCSGG 1 cut(s) 211
BsaAI YACGTR 1 cut(s) 688
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 6 cut(s) 114, 129, 181, 496, 606, 946
BsaXI ACNNNNNCTCC 6 cut(s) 492, 522, 555, 585, 695, 725
Bsc4I CCNNNNNNNGG 3 cut(s) 612, 874, 952
Bse1I ACTGG 1 cut(s) 835
Bse3DI GCAATG 2 cut(s) 69, 513
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 6 cut(s) 114, 129, 181, 496, 606, 946
BseGI GGATG 4 cut(s) 144, 188, 576, 742
BseLI CCNNNNNNNGG 3 cut(s) 612, 874, 952
BseMI GCAATG 2 cut(s) 69, 513
BseMII CTCAG 1 cut(s) 184
BseNI ACTGG 1 cut(s) 835
BseRI GAGGAG 1 cut(s) 628
BseX3I CGGCCG 1 cut(s) 493
Bsh1236I CGCG 3 cut(s) 275, 318, 498
Bsh1285I CGRYCG 1 cut(s) 496
BshFI GGCC 5 cut(s) 315, 495, 570, 729, 749
BsiEI CGRYCG 1 cut(s) 496
BsiHKAI GWGCWC 1 cut(s) 620
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 3 cut(s) 612, 874, 952
BsmAI GTCTC 2 cut(s) 139, 828
BsmFI GGGAC 1 cut(s) 394
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 5 cut(s) 315, 495, 570, 729, 749
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 620
Bsp143I GATC 4 cut(s) 185, 444, 697, 1021
Bsp19I CCATGG 2 cut(s) 129, 606
BspACI CCGC 5 cut(s) 275, 316, 496, 498, 899
BspANI GGCC 5 cut(s) 315, 495, 570, 729, 749
BspCNI CTCAG 1 cut(s) 183
BspFNI CGCG 3 cut(s) 275, 318, 498
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 4 cut(s) 382, 383, 502, 679
BspMAI CTGCAG 1 cut(s) 760
BspPI GGATC 2 cut(s) 193, 439
BspTNI GGTCTC 1 cut(s) 139
BsrDI GCAATG 2 cut(s) 69, 513
BsrI ACTGG 1 cut(s) 835
BssECI CCNNGG 6 cut(s) 114, 129, 181, 496, 606, 946
BssMI GATC 4 cut(s) 185, 444, 697, 1021
BssSI CACGAG 1 cut(s) 731
BssT1I CCWWGG 2 cut(s) 129, 606
Bst2BI CACGAG 1 cut(s) 731
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 8 cut(s) 100, 230, 624, 656, 856, 914, 950, 998
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 895
BstBAI YACGTR 1 cut(s) 688
BstC8I GCNNGC 2 cut(s) 529, 590
BstDEI CTNAG 1 cut(s) 170
BstDSI CCRYGG 4 cut(s) 129, 496, 606, 946
BstF5I GGATG 4 cut(s) 144, 188, 576, 742
BstFNI CGCG 3 cut(s) 275, 318, 498
BstH2I RGCGCY 2 cut(s) 271, 328
BstHHI GCGC 4 cut(s) 270, 284, 327, 722
BstKTI GATC 4 cut(s) 188, 447, 700, 1024
BstMAI GTCTC 2 cut(s) 139, 828
BstMBI GATC 4 cut(s) 185, 444, 697, 1021
BstMCI CGRYCG 1 cut(s) 496
BstMWI GCNNNNNNNGC 7 cut(s) 281, 324, 524, 726, 755, 895, 907
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 2 cut(s) 531, 847
BstSCI CCNGG 2 cut(s) 180, 209
BstSFI CTRYAG 2 cut(s) 756, 940
BstUI CGCG 3 cut(s) 275, 318, 498
BstZI CGGCCG 1 cut(s) 493
BsuRI GGCC 5 cut(s) 315, 495, 570, 729, 749
BtgI CCRYGG 4 cut(s) 129, 496, 606, 946
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 4 cut(s) 144, 188, 576, 742
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 3 cut(s) 12, 339, 1003
Cac8I GCNNGC 2 cut(s) 529, 590
CciI TCATGA 1 cut(s) 468
CfoI GCGC 4 cut(s) 270, 284, 327, 722
Cfr13I GGNCC 1 cut(s) 381
Cfr42I CCGCGG 1 cut(s) 499
Csp6I GTAC 1 cut(s) 253
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 1 cut(s) 170
DpnI GATC 4 cut(s) 187, 446, 699, 1023
DpnII GATC 4 cut(s) 185, 444, 697, 1021
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 1 cut(s) 493
EagI CGGCCG 1 cut(s) 493
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 618
EclXI CGGCCG 1 cut(s) 493
Eco130I CCWWGG 2 cut(s) 129, 606
Eco24I GRGCYC 1 cut(s) 620
Eco31I GGTCTC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 381
Eco52I CGGCCG 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 618
Eco72I CACGTG 1 cut(s) 688
Eco88I CYCGRG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 381
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 180
EcoT14I CCWWGG 2 cut(s) 129, 606
EcoT22I ATGCAT 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 620
ErhI CCWWGG 2 cut(s) 129, 606
FaqI GGGAC 1 cut(s) 394
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 3 cut(s) 316, 496, 899
FokI GGATG 4 cut(s) 131, 175, 563, 749
FriOI GRGCYC 1 cut(s) 620
Fsp4HI GCNGC 3 cut(s) 316, 496, 899
FspBI CTAG 1 cut(s) 348
GlaI GCGC 4 cut(s) 269, 283, 326, 721
GluI GCNGC 3 cut(s) 316, 496, 899
GsuI CTGGAG 3 cut(s) 164, 664, 852
HaeII RGCGCY 2 cut(s) 271, 328
HaeIII GGCC 5 cut(s) 315, 495, 570, 729, 749
HapII CCGG 1 cut(s) 210
HhaI GCGC 4 cut(s) 270, 284, 327, 722
Hin6I GCGC 4 cut(s) 268, 282, 325, 720
HinP1I GCGC 4 cut(s) 268, 282, 325, 720
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 3 cut(s) 242, 344, 821
HpaII CCGG 1 cut(s) 210
HphI GGTGA 5 cut(s) 454, 612, 622, 668, 940
Hpy166II GTNNAC 3 cut(s) 367, 511, 931
Hpy188I TCNGA 2 cut(s) 264, 409
Hpy188III TCNNGA 3 cut(s) 379, 469, 550
Hpy8I GTNNAC 3 cut(s) 367, 511, 931
Hpy99I CGWCG 5 cut(s) 227, 323, 359, 494, 728
HpyAV CCTTC 3 cut(s) 395, 872, 995
HpyCH4III ACNGT 8 cut(s) 100, 230, 624, 656, 856, 914, 950, 998
HpyCH4IV ACGT 2 cut(s) 546, 687
HpyCH4V TGCA 8 cut(s) 18, 518, 531, 588, 758, 847, 889, 980
HpyF10VI GCNNNNNNNGC 7 cut(s) 281, 324, 524, 726, 755, 895, 907
HpyF3I CTNAG 1 cut(s) 170
HpySE526I ACGT 2 cut(s) 546, 687
HspAI GCGC 4 cut(s) 268, 282, 325, 720
KflI GGGWCCC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 697
KspI CCGCGG 1 cut(s) 499
Kzo9I GATC 4 cut(s) 185, 444, 697, 1021
LmnI GCTCC 4 cut(s) 460, 500, 615, 683
LweI GCATC 5 cut(s) 153, 165, 349, 649, 876
MaeI CTAG 1 cut(s) 348
MaeII ACGT 2 cut(s) 546, 687
MaeIII GTNAC 9 cut(s) 239, 337, 350, 434, 547, 624, 760, 812, 850
MalI GATC 4 cut(s) 187, 446, 699, 1023
MboI GATC 4 cut(s) 185, 444, 697, 1021
MboII GAAGA 1 cut(s) 46
MfeI CAATTG 2 cut(s) 247, 306
MhlI GDGCHC 1 cut(s) 620
MluCI AATT 5 cut(s) 84, 214, 247, 306, 775
MlyI GAGTC 3 cut(s) 236, 338, 815
MmeI TCCRAC 2 cut(s) 617, 738
Mph1103I ATGCAT 1 cut(s) 533
MseI TTAA 2 cut(s) 21, 372
MslI CAYNNNNRTG 5 cut(s) 199, 579, 792, 795, 848
MspA1I CMGCKG 1 cut(s) 498
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 182, 211
MunI CAATTG 2 cut(s) 247, 306
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 1 cut(s) 182
MvnI CGCG 3 cut(s) 275, 318, 498
MwoI GCNNNNNNNGC 7 cut(s) 281, 324, 524, 726, 755, 895, 907
NciI CCSGG 1 cut(s) 211
NcoI CCATGG 2 cut(s) 129, 606
NdeII GATC 4 cut(s) 185, 444, 697, 1021
NlaIV GGNNCC 4 cut(s) 382, 383, 502, 679
NmuCI GTSAC 8 cut(s) 239, 337, 350, 434, 547, 624, 812, 850
NsiI ATGCAT 1 cut(s) 533
NspI RCATGY 2 cut(s) 531, 847
PaeI GCATGC 1 cut(s) 531
PagI TCATGA 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 1 cut(s) 607
PkrI GCNGC 3 cut(s) 317, 497, 900
PleI GAGTC 3 cut(s) 236, 338, 815
PmaCI CACGTG 1 cut(s) 688
PmlI CACGTG 1 cut(s) 688
PpsI GAGTC 3 cut(s) 236, 338, 815
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 620
Psp5II RGGWCCY 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 180
PspCI CACGTG 1 cut(s) 688
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 4 cut(s) 382, 383, 502, 679
PspPI GGNCC 1 cut(s) 381
PspPPI RGGWCCY 1 cut(s) 381
PsrI GAACNNNNNNTAC 2 cut(s) 923, 955
PstI CTGCAG 1 cut(s) 760
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
RseI CAYNNNNRTG 5 cut(s) 199, 579, 792, 795, 848
SacI GAGCTC 1 cut(s) 620
SacII CCGCGG 1 cut(s) 499
SaqAI TTAA 2 cut(s) 21, 372
SatI GCNGC 3 cut(s) 316, 496, 899
Sau3AI GATC 4 cut(s) 185, 444, 697, 1021
Sau96I GGNCC 1 cut(s) 381
SchI GAGTC 3 cut(s) 236, 338, 815
ScrFI CCNGG 2 cut(s) 182, 211
SduI GDGCHC 1 cut(s) 620
SfaNI GCATC 5 cut(s) 153, 165, 349, 649, 876
SfcI CTRYAG 2 cut(s) 756, 940
Sfr303I CCGCGG 1 cut(s) 499
SgrBI CCGCGG 1 cut(s) 499
SinI GGWCC 1 cut(s) 381
SmiMI CAYNNNNRTG 5 cut(s) 199, 579, 792, 795, 848
SmlI CTYRAG 2 cut(s) 139, 452
SmoI CTYRAG 2 cut(s) 139, 452
SphI GCATGC 1 cut(s) 531
Sse9I AATT 5 cut(s) 84, 214, 247, 306, 775
SsiI CCGC 5 cut(s) 275, 316, 496, 498, 899
SspMI CTAG 1 cut(s) 348
SstI GAGCTC 1 cut(s) 620
StyD4I CCNGG 2 cut(s) 180, 209
StyI CCWWGG 2 cut(s) 129, 606
TaaI ACNGT 8 cut(s) 100, 230, 624, 656, 856, 914, 950, 998
TaiI ACGT 2 cut(s) 549, 690
TaqI TCGA 5 cut(s) 102, 159, 222, 489, 668
TasI AATT 5 cut(s) 84, 214, 247, 306, 775
TauI GCSGC 3 cut(s) 318, 498, 901
Tru1I TTAA 2 cut(s) 21, 372
Tru9I TTAA 2 cut(s) 21, 372
TscAI CASTG 3 cut(s) 12, 346, 1003
TseFI GTSAC 8 cut(s) 239, 337, 350, 434, 547, 624, 812, 850
Tsp45I GTSAC 8 cut(s) 239, 337, 350, 434, 547, 624, 812, 850
TspDTI ATGAA 3 cut(s) 58, 485, 537
TspRI CASTG 3 cut(s) 12, 346, 1003
VpaK11BI GGWCC 1 cut(s) 381
XapI RAATTY 1 cut(s) 214
XceI RCATGY 2 cut(s) 531, 847
XspI CTAG 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.