RLG00000025755

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010647
Physical Location & Seq
Reverse (-)
48090397 .. 48091689
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025755

Sequence Viewer

Length: 1293 bp
ATGGCAGTGTTTGAGTGCGTTAAGAGAATGATTTATATGTGTATATTGGTTCTCTTCCTGTTCGGCTTCATTGCCGAAACACGAACTGCCCCAACAACAGTCGATGACGACCCCGAGGAAGTTGCTTCCATGGTCTCCTTGAGCATCCAAAACAGCATCGAAAAAAAGCTCAGGCAACTCCAGGGATCATCCTCATTATCATGTGCTACCGGGAATTCCATCGACGACTGTTGGCGATGTGACTCCAATTGGTACAACAATCGGAAGCGCCTCGCGGACTGCGCTATTGGTTTCGGCAGGAACACAATTGGAGGCCGCGACGGGCGCTTCTACATCGTCACTGACTCTAGTGACGACGATGCTGTCAACCTTAAAGCCGGGACCCTTCGCCACGCTATAATACAATCCGAGCCTCTATGGATTGTGTTCCAGCGTGACATGGTGATCCAGCTCAAGCAGGAGCTTATCATGAATAGCTATAAAACCATCGACGGCCGCGGAGCCAATGTTCACATTGCAAATGAAGCATGCATTACAATCCAAAACGTCACGAACATCATAATACATGGCCTCCACATCCATGACTGCAAGCCTACAGGGAATGCCATGGTGAGGAGCTCACCGTCACACTTTGGTTGGAGAGGAATGGCAGACGGTGATGCTGTGTCGATTTTCGGCTCCAGTAACGTGTGGATTGATCACAACTCGCTCTCTAACTGCGCCGACGGCCTCGTGGATGTTGTCATGGCCTCAACTGCAGTTACAATCTCTAATAATTACTTCTCGCACCACAATGATGTCTTGTTGTTTGGTCACAGCGACTCTTATACTGGAGACAAGGACATGCAAGTGACGGTTGCTTACAACCACTTTGGGGAAGGATTGATGCAAAGATTGCCGCATTGTAGGCACGGCTATTGTCATGTGGTGAACAATGACTACAGCCACGGTTGGGGGATATACGCCATCGGTGGAGGTGCAAACCCTACCATCAACAGCGAAGGCAACCGATTTGTTGCTGATCTGAAAAGGTACCCGGATTCGAGTGAGGTTACCAAGAGGACTTCGGGAGACAAGTCGGAGTGGAAGAACTGGAATTGGATCTCTCAGGGAGATTTGTTCTTGAATGGTGCCTATTTTGTTCCGTCTGGAGCAACCGGAGCTAACTCGGAGGCCTATGCTAAGGCCTCGAGCTATGCAAAAGCTAAGAAATCTTCCATGGTTGGAGCCATAACTTCTGCTGCTGGTGCACTCACCTGCCGCAAAGACCATATGTGCACTAGTAACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

431

Amino Acids

47.19

Weight (kDa)

6.34

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 152 - 358 7.2e-22 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1265
AasI GACNNNNNNGTC 1 cut(s) 362
Acc36I ACCTGC 1 cut(s) 1265
Acc65I GGTACC 1 cut(s) 1032
AccB1I GGYRCC 2 cut(s) 1032, 1130
AccII CGCG 3 cut(s) 275, 318, 498
AciI CCGC 6 cut(s) 275, 316, 496, 498, 899, 1261
AclWI GGATC 3 cut(s) 193, 439, 1109
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 1 cut(s) 214
AfaI GTAC 2 cut(s) 254, 1034
AfiI CCNNNNNNNGG 3 cut(s) 612, 874, 952
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 1 cut(s) 1126
AhlI ACTAGT 1 cut(s) 1280
AjnI CCWGG 1 cut(s) 180
AloI GAACNNNNNNTCC 2 cut(s) 492, 524
AluBI AGCT 9 cut(s) 169, 451, 463, 477, 618, 1163, 1194, 1205, 1290
AluI AGCT 9 cut(s) 169, 451, 463, 477, 618, 1163, 1194, 1205, 1290
Alw21I GWGCWC 3 cut(s) 620, 1252, 1280
Alw26I GTCTC 3 cut(s) 139, 828, 1065
Alw44I GTGCAC 2 cut(s) 1248, 1276
AlwI GGATC 3 cut(s) 193, 439, 1109
Ama87I CYCGRG 2 cut(s) 113, 1189
AoxI GGCC 7 cut(s) 313, 493, 568, 727, 747, 1173, 1185
ApaLI GTGCAC 2 cut(s) 1248, 1276
ApeKI GCWGC 1 cut(s) 1241
ApoI RAATTY 1 cut(s) 214
Asp718I GGTACC 1 cut(s) 1032
AspLEI GCGC 4 cut(s) 270, 284, 327, 722
AspS9I GGNCC 1 cut(s) 381
AsuC2I CCSGG 3 cut(s) 211, 379, 1037
AsuHPI GGTGA 6 cut(s) 454, 612, 622, 668, 940, 1246
AvaI CYCGRG 2 cut(s) 113, 1189
AvaII GGWCC 1 cut(s) 381
BaeGI GKGCMC 2 cut(s) 1252, 1280
BanI GGYRCC 2 cut(s) 1032, 1130
BanII GRGCYC 1 cut(s) 620
BauI CACGAG 1 cut(s) 731
Bbv12I GWGCWC 3 cut(s) 620, 1252, 1280
BbvI GCAGC 1 cut(s) 1228
BccI CCATC 4 cut(s) 227, 494, 974, 998
BceAI ACGGC 3 cut(s) 508, 742, 928
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 1 cut(s) 182
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 3 cut(s) 211, 379, 1037
BcoDI GTCTC 3 cut(s) 139, 828, 1065
BcuI ACTAGT 1 cut(s) 1280
BfaI CTAG 3 cut(s) 348, 1281, 1291
BfmI CTRYAG 3 cut(s) 594, 756, 940
BfoI RGCGCY 2 cut(s) 271, 328
BfuAI ACCTGC 1 cut(s) 1265
BisI GCNGC 5 cut(s) 316, 496, 899, 1242, 1261
BlsI GCNGC 5 cut(s) 317, 497, 900, 1243, 1262
Bme1390I CCNGG 4 cut(s) 182, 211, 379, 1037
Bme18I GGWCC 1 cut(s) 381
BmeT110I CYCGRG 2 cut(s) 113, 1189
BmgT120I GGNCC 1 cut(s) 381
BmiI GGNNCC 7 cut(s) 382, 383, 502, 679, 1034, 1132, 1228
BmrFI CCNGG 4 cut(s) 182, 211, 379, 1037
BmsI GCATC 5 cut(s) 153, 165, 349, 649, 876
BpmI CTGGAG 4 cut(s) 164, 664, 852, 1170
Bpu10I CCTNAGC 2 cut(s) 170, 1182
BpuEI CTTGAG 2 cut(s) 160, 437
BpuMI CCSGG 3 cut(s) 211, 379, 1037
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 7 cut(s) 114, 129, 181, 496, 606, 946, 1218
BsaWI WCCGGW 1 cut(s) 1157
BsaXI ACNNNNNCTCC 4 cut(s) 492, 522, 555, 585
Bsc4I CCNNNNNNNGG 3 cut(s) 612, 874, 952
Bse1I ACTGG 3 cut(s) 681, 835, 1097
Bse3DI GCAATG 2 cut(s) 69, 513
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 7 cut(s) 114, 129, 181, 496, 606, 946, 1218
BseGI GGATG 4 cut(s) 144, 188, 576, 742
BseLI CCNNNNNNNGG 3 cut(s) 612, 874, 952
BseMI GCAATG 2 cut(s) 69, 513
BseMII CTCAG 2 cut(s) 184, 1121
BseNI ACTGG 3 cut(s) 681, 835, 1097
BseRI GAGGAG 1 cut(s) 628
BseSI GKGCMC 2 cut(s) 1252, 1280
BseX3I CGGCCG 1 cut(s) 493
BseXI GCAGC 1 cut(s) 1228
Bsh1236I CGCG 3 cut(s) 275, 318, 498
Bsh1285I CGRYCG 1 cut(s) 496
BshFI GGCC 7 cut(s) 315, 495, 570, 729, 749, 1175, 1187
BshNI GGYRCC 2 cut(s) 1032, 1130
BsiEI CGRYCG 1 cut(s) 496
BsiHKAI GWGCWC 3 cut(s) 620, 1252, 1280
BsiHKCI CYCGRG 2 cut(s) 113, 1189
BsiSI CCGG 4 cut(s) 210, 378, 1037, 1158
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 3 cut(s) 612, 874, 952
BsmAI GTCTC 3 cut(s) 139, 828, 1065
BsmFI GGGAC 1 cut(s) 394
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 7 cut(s) 315, 495, 570, 729, 749, 1175, 1187
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 2 cut(s) 113, 1189
Bsp1286I GDGCHC 3 cut(s) 620, 1252, 1280
Bsp143I GATC 5 cut(s) 185, 444, 697, 1021, 1101
Bsp19I CCATGG 3 cut(s) 129, 606, 1218
BspACI CCGC 6 cut(s) 275, 316, 496, 498, 899, 1261
BspANI GGCC 7 cut(s) 315, 495, 570, 729, 749, 1175, 1187
BspCNI CTCAG 2 cut(s) 183, 1120
BspFNI CGCG 3 cut(s) 275, 318, 498
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 7 cut(s) 382, 383, 502, 679, 1034, 1132, 1228
BspMAI CTGCAG 1 cut(s) 760
BspMI ACCTGC 1 cut(s) 1265
BspPI GGATC 3 cut(s) 193, 439, 1109
BspT107I GGYRCC 2 cut(s) 1032, 1130
BspTNI GGTCTC 1 cut(s) 139
BsrDI GCAATG 2 cut(s) 69, 513
BsrI ACTGG 3 cut(s) 681, 835, 1097
BssECI CCNNGG 7 cut(s) 114, 129, 181, 496, 606, 946, 1218
BssMI GATC 5 cut(s) 185, 444, 697, 1021, 1101
BssSI CACGAG 1 cut(s) 731
BssT1I CCWWGG 3 cut(s) 129, 606, 1218
Bst2BI CACGAG 1 cut(s) 731
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 6 cut(s) 100, 230, 624, 656, 856, 950
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 895
BstC8I GCNNGC 2 cut(s) 529, 590
BstDEI CTNAG 4 cut(s) 170, 1107, 1182, 1206
BstDSI CCRYGG 5 cut(s) 129, 496, 606, 946, 1218
BstEII GGTNACC 1 cut(s) 1051
BstF5I GGATG 4 cut(s) 144, 188, 576, 742
BstFNI CGCG 3 cut(s) 275, 318, 498
BstH2I RGCGCY 2 cut(s) 271, 328
BstHHI GCGC 4 cut(s) 270, 284, 327, 722
BstKTI GATC 5 cut(s) 188, 447, 700, 1024, 1104
BstMAI GTCTC 3 cut(s) 139, 828, 1065
BstMBI GATC 5 cut(s) 185, 444, 697, 1021, 1101
BstMCI CGRYCG 1 cut(s) 496
BstMWI GCNNNNNNNGC 9 cut(s) 281, 324, 524, 726, 755, 895, 907, 1160, 1247
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 2 cut(s) 531, 847
BstPI GGTNACC 1 cut(s) 1051
BstSCI CCNGG 4 cut(s) 180, 209, 377, 1035
BstSFI CTRYAG 3 cut(s) 594, 756, 940
BstSLI GKGCMC 2 cut(s) 1252, 1280
BstUI CGCG 3 cut(s) 275, 318, 498
BstV1I GCAGC 1 cut(s) 1228
BstX2I RGATCY 1 cut(s) 1101
BstYI RGATCY 1 cut(s) 1101
BstZI CGGCCG 1 cut(s) 493
BsuRI GGCC 7 cut(s) 315, 495, 570, 729, 749, 1175, 1187
BtgI CCRYGG 5 cut(s) 129, 496, 606, 946, 1218
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 4 cut(s) 144, 188, 576, 742
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 2 cut(s) 12, 339
BveI ACCTGC 1 cut(s) 1265
Cac8I GCNNGC 2 cut(s) 529, 590
CciI TCATGA 1 cut(s) 468
CfoI GCGC 4 cut(s) 270, 284, 327, 722
Cfr13I GGNCC 1 cut(s) 381
Cfr42I CCGCGG 1 cut(s) 499
Csp6I GTAC 2 cut(s) 253, 1033
CviQI GTAC 2 cut(s) 253, 1033
DdeI CTNAG 4 cut(s) 170, 1107, 1182, 1206
DpnI GATC 5 cut(s) 187, 446, 699, 1023, 1103
DpnII GATC 5 cut(s) 185, 444, 697, 1021, 1101
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 1 cut(s) 493
EagI CGGCCG 1 cut(s) 493
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 618
EclXI CGGCCG 1 cut(s) 493
Eco130I CCWWGG 3 cut(s) 129, 606, 1218
Eco147I AGGCCT 2 cut(s) 1175, 1187
Eco24I GRGCYC 1 cut(s) 620
Eco31I GGTCTC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 381
Eco52I CGGCCG 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 618
Eco88I CYCGRG 2 cut(s) 113, 1189
Eco91I GGTNACC 1 cut(s) 1051
EcoICRI GAGCTC 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 381
EcoO65I GGTNACC 1 cut(s) 1051
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 180
EcoT14I CCWWGG 3 cut(s) 129, 606, 1218
EcoT22I ATGCAT 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 620
ErhI CCWWGG 3 cut(s) 129, 606, 1218
FaqI GGGAC 1 cut(s) 394
FauNDI CATATG 1 cut(s) 1272
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 5 cut(s) 316, 496, 899, 1242, 1261
FokI GGATG 4 cut(s) 131, 175, 563, 749
FriOI GRGCYC 1 cut(s) 620
Fsp4HI GCNGC 5 cut(s) 316, 496, 899, 1242, 1261
FspBI CTAG 3 cut(s) 348, 1281, 1291
GlaI GCGC 4 cut(s) 269, 283, 326, 721
GluI GCNGC 5 cut(s) 316, 496, 899, 1242, 1261
GsuI CTGGAG 4 cut(s) 164, 664, 852, 1170
HaeII RGCGCY 2 cut(s) 271, 328
HaeIII GGCC 7 cut(s) 315, 495, 570, 729, 749, 1175, 1187
HapII CCGG 4 cut(s) 210, 378, 1037, 1158
HhaI GCGC 4 cut(s) 270, 284, 327, 722
Hin6I GCGC 4 cut(s) 268, 282, 325, 720
HinP1I GCGC 4 cut(s) 268, 282, 325, 720
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 4 cut(s) 242, 344, 821, 1040
HpaII CCGG 4 cut(s) 210, 378, 1037, 1158
HphI GGTGA 6 cut(s) 454, 612, 622, 668, 940, 1246
Hpy166II GTNNAC 5 cut(s) 367, 511, 931, 1250, 1278
Hpy188I TCNGA 5 cut(s) 264, 409, 1026, 1081, 1171
Hpy188III TCNNGA 5 cut(s) 469, 550, 1068, 1123, 1149
Hpy8I GTNNAC 5 cut(s) 367, 511, 931, 1250, 1278
Hpy99I CGWCG 5 cut(s) 227, 323, 359, 494, 728
HpyAV CCTTC 3 cut(s) 395, 872, 995
HpyCH4III ACNGT 6 cut(s) 100, 230, 624, 656, 856, 950
HpyCH4IV ACGT 2 cut(s) 546, 687
HpyF10VI GCNNNNNNNGC 9 cut(s) 281, 324, 524, 726, 755, 895, 907, 1160, 1247
HpyF3I CTNAG 4 cut(s) 170, 1107, 1182, 1206
HpySE526I ACGT 2 cut(s) 546, 687
HspAI GCGC 4 cut(s) 268, 282, 325, 720
KflI GGGWCCC 1 cut(s) 381
KpnI GGTACC 1 cut(s) 1036
Ksp22I TGATCA 1 cut(s) 697
KspI CCGCGG 1 cut(s) 499
Kzo9I GATC 5 cut(s) 185, 444, 697, 1021, 1101
LmnI GCTCC 7 cut(s) 460, 500, 615, 683, 1151, 1160, 1226
Lsp1109I GCAGC 1 cut(s) 1228
LweI GCATC 5 cut(s) 153, 165, 349, 649, 876
MaeI CTAG 3 cut(s) 348, 1281, 1291
MaeII ACGT 2 cut(s) 546, 687
MalI GATC 5 cut(s) 187, 446, 699, 1023, 1103
MboI GATC 5 cut(s) 185, 444, 697, 1021, 1101
MboII GAAGA 3 cut(s) 46, 1099, 1206
MfeI CAATTG 2 cut(s) 247, 306
MflI RGATCY 1 cut(s) 1101
MhlI GDGCHC 3 cut(s) 620, 1252, 1280
MluCI AATT 5 cut(s) 214, 247, 306, 775, 1096
MlyI GAGTC 3 cut(s) 236, 338, 815
MmeI TCCRAC 3 cut(s) 617, 1059, 1204
Mph1103I ATGCAT 1 cut(s) 533
MseI TTAA 2 cut(s) 21, 372
MslI CAYNNNNRTG 5 cut(s) 199, 579, 792, 795, 848
MspA1I CMGCKG 1 cut(s) 498
MspI CCGG 4 cut(s) 210, 378, 1037, 1158
MspR9I CCNGG 4 cut(s) 182, 211, 379, 1037
MunI CAATTG 2 cut(s) 247, 306
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 1 cut(s) 182
MvnI CGCG 3 cut(s) 275, 318, 498
MwoI GCNNNNNNNGC 9 cut(s) 281, 324, 524, 726, 755, 895, 907, 1160, 1247
NciI CCSGG 3 cut(s) 211, 379, 1037
NcoI CCATGG 3 cut(s) 129, 606, 1218
NdeI CATATG 1 cut(s) 1272
NdeII GATC 5 cut(s) 185, 444, 697, 1021, 1101
NlaIV GGNNCC 7 cut(s) 382, 383, 502, 679, 1034, 1132, 1228
NmuCI GTSAC 8 cut(s) 239, 337, 350, 434, 547, 624, 812, 850
NsiI ATGCAT 1 cut(s) 533
NspI RCATGY 2 cut(s) 531, 847
PaeI GCATGC 1 cut(s) 531
PaeR7I CTCGAG 1 cut(s) 1189
PagI TCATGA 1 cut(s) 468
PaqCI CACCTGC 1 cut(s) 1265
PceI AGGCCT 2 cut(s) 1175, 1187
PctI GAATGC 1 cut(s) 607
PfeI GAWTC 1 cut(s) 1040
PkrI GCNGC 5 cut(s) 317, 497, 900, 1243, 1262
PleI GAGTC 3 cut(s) 236, 338, 815
PpsI GAGTC 3 cut(s) 236, 338, 815
PpuMI RGGWCCY 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 620
Psp5II RGGWCCY 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 180
PspEI GGTNACC 1 cut(s) 1051
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 7 cut(s) 382, 383, 502, 679, 1034, 1132, 1228
PspPI GGNCC 1 cut(s) 381
PspPPI RGGWCCY 1 cut(s) 381
PspXI VCTCGAGB 1 cut(s) 1189
PsrI GAACNNNNNNTAC 2 cut(s) 923, 955
PstI CTGCAG 1 cut(s) 760
PsuI RGATCY 1 cut(s) 1101
RsaI GTAC 2 cut(s) 254, 1034
RsaNI GTAC 2 cut(s) 253, 1033
RseI CAYNNNNRTG 5 cut(s) 199, 579, 792, 795, 848
SacI GAGCTC 1 cut(s) 620
SacII CCGCGG 1 cut(s) 499
SaqAI TTAA 2 cut(s) 21, 372
SatI GCNGC 5 cut(s) 316, 496, 899, 1242, 1261
Sau3AI GATC 5 cut(s) 185, 444, 697, 1021, 1101
Sau96I GGNCC 1 cut(s) 381
SchI GAGTC 3 cut(s) 236, 338, 815
ScrFI CCNGG 4 cut(s) 182, 211, 379, 1037
SduI GDGCHC 3 cut(s) 620, 1252, 1280
SfaNI GCATC 5 cut(s) 153, 165, 349, 649, 876
SfcI CTRYAG 3 cut(s) 594, 756, 940
Sfr274I CTCGAG 1 cut(s) 1189
Sfr303I CCGCGG 1 cut(s) 499
SgrBI CCGCGG 1 cut(s) 499
SinI GGWCC 1 cut(s) 381
SlaI CTCGAG 1 cut(s) 1189
SmiMI CAYNNNNRTG 5 cut(s) 199, 579, 792, 795, 848
SmlI CTYRAG 3 cut(s) 139, 452, 1189
SmoI CTYRAG 3 cut(s) 139, 452, 1189
SpeI ACTAGT 1 cut(s) 1280
SphI GCATGC 1 cut(s) 531
Sse9I AATT 5 cut(s) 214, 247, 306, 775, 1096
SseBI AGGCCT 2 cut(s) 1175, 1187
SsiI CCGC 6 cut(s) 275, 316, 496, 498, 899, 1261
SspMI CTAG 3 cut(s) 348, 1281, 1291
SstI GAGCTC 1 cut(s) 620
StuI AGGCCT 2 cut(s) 1175, 1187
StyD4I CCNGG 4 cut(s) 180, 209, 377, 1035
StyI CCWWGG 3 cut(s) 129, 606, 1218
TaaI ACNGT 6 cut(s) 100, 230, 624, 656, 856, 950
TaiI ACGT 2 cut(s) 549, 690
TaqI TCGA 7 cut(s) 102, 159, 222, 489, 668, 1043, 1190
TasI AATT 5 cut(s) 214, 247, 306, 775, 1096
TauI GCSGC 4 cut(s) 318, 498, 901, 1263
TfiI GAWTC 1 cut(s) 1040
Tru1I TTAA 2 cut(s) 21, 372
Tru9I TTAA 2 cut(s) 21, 372
TscAI CASTG 2 cut(s) 12, 346
TseFI GTSAC 8 cut(s) 239, 337, 350, 434, 547, 624, 812, 850
TseI GCWGC 1 cut(s) 1241
Tsp45I GTSAC 8 cut(s) 239, 337, 350, 434, 547, 624, 812, 850
TspDTI ATGAA 3 cut(s) 58, 485, 537
TspGWI ACGGA 1 cut(s) 1134
TspRI CASTG 2 cut(s) 12, 346
VneI GTGCAC 2 cut(s) 1248, 1276
VpaK11BI GGWCC 1 cut(s) 381
XapI RAATTY 1 cut(s) 214
XceI RCATGY 2 cut(s) 531, 847
XhoI CTCGAG 1 cut(s) 1189
XspI CTAG 3 cut(s) 348, 1281, 1291
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.